Pub Date : 2025-10-13DOI: 10.1016/j.jobcr.2025.09.017
Mauli Alkesh Mody , Pritesh B. Ruparelia , Manali Patel , Kosha P. Ruparelia
Background
The present study was conducted to evaluate and compare selective salivary properties and taste perception in subjects with and without oral submucous fibrosis (OSMF), since OSMF affects the properties of saliva and may lead to decline in the quality of life.
Materials and method
Eighty subjects were enrolled in the study and equally divided into two groups based on the presence or absence of OSMF, for estimating, analysing and comparing the salivary flow rate (SFR), pH and taste perception using Modified Schirmer strip, pH strip and taste solutions respectively, in both groups. Chi-square test and unpaired t-test were applied.
Results
Compared to the control group, a statistically significant decrease in SFR was noted among stage 4 OSMF subjects. Alkaline pH was present in OSMF group, regardless of the stage. Taste alterations were present in relation to sweet, salty and sour taste in subjects with OSMF.
Conclusion
There is a marked decrease in SFR, alteration in salivary pH and significant taste changes such as hypogeusia, dysgeusia and ageusia in sweet, salt and sour tastes in subjects with OSMF.
{"title":"Assessment of selective salivary properties and taste perception in subjects with oral submucous fibrosis – A case-control study","authors":"Mauli Alkesh Mody , Pritesh B. Ruparelia , Manali Patel , Kosha P. Ruparelia","doi":"10.1016/j.jobcr.2025.09.017","DOIUrl":"10.1016/j.jobcr.2025.09.017","url":null,"abstract":"<div><h3>Background</h3><div>The present study was conducted to evaluate and compare selective salivary properties and taste perception in subjects with and without oral submucous fibrosis (OSMF), since OSMF affects the properties of saliva and may lead to decline in the quality of life.</div></div><div><h3>Materials and method</h3><div>Eighty subjects were enrolled in the study and equally divided into two groups based on the presence or absence of OSMF, for estimating, analysing and comparing the salivary flow rate (SFR), pH and taste perception using Modified Schirmer strip, pH strip and taste solutions respectively, in both groups. Chi-square test and unpaired <em>t</em>-test were applied.</div></div><div><h3>Results</h3><div>Compared to the control group, a statistically significant decrease in SFR was noted among stage 4 OSMF subjects. Alkaline pH was present in OSMF group, regardless of the stage. Taste alterations were present in relation to sweet, salty and sour taste in subjects with OSMF.</div></div><div><h3>Conclusion</h3><div>There is a marked decrease in SFR, alteration in salivary pH and significant taste changes such as hypogeusia, dysgeusia and ageusia in sweet, salt and sour tastes in subjects with OSMF.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1676-1680"},"PeriodicalIF":0.0,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145325166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-10DOI: 10.1016/j.jobcr.2025.09.029
Preethesh Shetty, Raksha Bhat, Shishir Shetty
Background
DNA extraction from infected dental pulp tissue represents a critical methodological limitation in molecular endodontics, severely constraining pathogen identification and precision therapeutic approaches. Conventional extraction protocols demonstrate systematic failures when applied to inflamed pulp samples containing complex hydroxyapatite-collagen matrices, neutrophil extracellular traps, and inflammatory mediators that compromise nucleic acid integrity and downstream next-generation sequencing applications.
Methods
The present investigation comprehensively validated a thermomechanical extraction protocol combining optimized extended thermal incubation with intensive mechanical disruption cycles specifically designed for infected dental pulp tissues. Performance was systematically evaluated against the current standardised systems using multi-parameter quality assessment, statistical analysis including effect size calculations across 24 infected pulp samples from patients with irreversible pulpitis.
Results
The thermomechanical protocol achieved transformative improvements across all performance metrics: 3.7-fold enhancement in DNA concentration (69.8 ± 10.21 vs. 18.83 ± 12.72 ng/μL, p < 0.01, Cohen's d = 4.2), 18 % improvement in protein purity ratios (A260/A280: 2.23 ± 0.23 vs. 1.89 ± 0.060, p < 0.01), and unprecedented 4–6 fold enhancement in inter-sample reproducibility (coefficient of variation reduction from 67.6 % to 14.6 %). Universal optimal quality classification was achieved (100 % vs. 58.3 % for conventional protocols), enabling reliable endodontic microbiome analysis and pulp genomics applications.
Conclusions
The current thermomechanical approach establishes performance standards for infected dental pulp DNA extraction, providing reliable methodology for endodontic microbiome analysis, pulp-dentin genomics, and precision therapy selection. The superior reproducibility and pathophysiology-specific refinement positions it as essential for advancing molecular endodontics and evidence-based therapeutic decision-making.
从感染牙髓组织中提取dna是分子牙髓学中一个关键的方法学限制，严重限制了病原体鉴定和精确治疗方法。传统的提取方法在处理含有复杂羟基磷灰石-胶原基质、中性粒细胞胞外陷阱和炎症介质的炎症牙髓样品时显示出系统性的失败，这些物质会损害核酸的完整性和下游下一代测序的应用。方法本研究综合验证了针对感染牙髓组织设计的热机械提取方案，该方案结合了优化的延长热培养和强化的机械破坏循环。采用多参数质量评估和统计分析，包括对来自不可逆牙髓炎患者的24个感染牙髓样本的效应量计算，对当前标准化系统的性能进行系统评估。结果热机械方案在所有性能指标上都取得了革命性的改善：DNA浓度提高3.7倍（69.8±10.21 vs. 18.83±12.72 ng/μL, p < 0.01, Cohen’s d = 4.2），蛋白质纯度比提高18% (A260/A280: 2.23±0.23 vs. 1.89±0.060,p < 0.01)，样品间可重复性提高4-6倍（变异系数从67.6%降低到14.6%）。实现了普遍的最佳质量分类（100%，而传统方案为58.3%），实现了可靠的牙髓微生物组分析和牙髓基因组学应用。结论热力学方法建立了感染牙髓DNA提取的性能标准，为牙髓微生物组分析、牙髓-牙本质基因组学和精确治疗选择提供了可靠的方法。优越的可重复性和病理生理特异性的细化定位它是必不可少的推进分子牙髓学和循证治疗决策。
{"title":"Thermomechanical DNA extraction from infected dental pulp for next-generation sequencing applications","authors":"Preethesh Shetty, Raksha Bhat, Shishir Shetty","doi":"10.1016/j.jobcr.2025.09.029","DOIUrl":"10.1016/j.jobcr.2025.09.029","url":null,"abstract":"<div><h3>Background</h3><div>DNA extraction from infected dental pulp tissue represents a critical methodological limitation in molecular endodontics, severely constraining pathogen identification and precision therapeutic approaches. Conventional extraction protocols demonstrate systematic failures when applied to inflamed pulp samples containing complex hydroxyapatite-collagen matrices, neutrophil extracellular traps, and inflammatory mediators that compromise nucleic acid integrity and downstream next-generation sequencing applications.</div></div><div><h3>Methods</h3><div>The present investigation comprehensively validated a thermomechanical extraction protocol combining optimized extended thermal incubation with intensive mechanical disruption cycles specifically designed for infected dental pulp tissues. Performance was systematically evaluated against the current standardised systems using multi-parameter quality assessment, statistical analysis including effect size calculations across 24 infected pulp samples from patients with irreversible pulpitis.</div></div><div><h3>Results</h3><div>The thermomechanical protocol achieved transformative improvements across all performance metrics: 3.7-fold enhancement in DNA concentration (69.8 ± 10.21 vs. 18.83 ± 12.72 ng/μL, p < 0.01, Cohen's d = 4.2), 18 % improvement in protein purity ratios (A<sub>260</sub>/A<sub>280</sub>: 2.23 ± 0.23 vs. 1.89 ± 0.060, p < 0.01), and unprecedented 4–6 fold enhancement in inter-sample reproducibility (coefficient of variation reduction from 67.6 % to 14.6 %). Universal optimal quality classification was achieved (100 % vs. 58.3 % for conventional protocols), enabling reliable endodontic microbiome analysis and pulp genomics applications.</div></div><div><h3>Conclusions</h3><div>The current thermomechanical approach establishes performance standards for infected dental pulp DNA extraction, providing reliable methodology for endodontic microbiome analysis, pulp-dentin genomics, and precision therapy selection. The superior reproducibility and pathophysiology-specific refinement positions it as essential for advancing molecular endodontics and evidence-based therapeutic decision-making.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1668-1675"},"PeriodicalIF":0.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To compare and evaluate the clinical efficacy of Hyaluronic Acid and Injectable Platelet Rich Fibrin (i-PRF) in the management of gingival black triangles using clinical and photographic analysis over 6 months.
Material and method
Thirty patients (15 in each group) were evaluated in this comparative study. Patients were divided into two groups: Group(HA) and Group(i-PRF). Injections were administered at baseline, 3 weeks, and 6 weeks. Black triangle height and area were measured at baseline, 1 month, 3 months, and 6 months using a UNC-15 probe and ImageJ software. Data were analyzed using repeated measures ANOVA and Tukey's post hoc test.
Results
Both the HA and i-PRF groups showed significant reductions in black triangle height and area over a 6-month period. HA reduced height by 0.80 mm at 1 month (p = 0.067), 1.47 mm at 3 months (p < 0.001), and 2.00 mm at 6 months (p < 0.001), while i-PRF reduced height by 1.20 mm (p = 0.003), 1.80 mm (p < 0.001), and 2.27 mm (p < 0.001) at the same intervals. Area reductions followed similar patterns. No significant intergroup differences were observed.
Conclusion
HA and i-PRF are effective, minimally invasive, and biocompatible treatments for managing black triangles. HA offers ease of use and patient comfort, while i-PRF is biologically driven and cost-effective. Material selection should be based on clinical conditions and patient preferences.
目的比较和评价透明质酸与富血小板纤维蛋白（i-PRF）治疗6个月牙龈黑三角的临床疗效。材料与方法30例患者（每组15例）进行比较研究。患者分为两组：HA组和i-PRF组。在基线、3周和6周进行注射。使用UNC-15探针和ImageJ软件在基线、1个月、3个月和6个月测量黑色三角形的高度和面积。数据分析采用重复测量方差分析和Tukey事后检验。结果在6个月的时间里，HA组和i-PRF组的黑三角高度和面积均有显著降低。HA在1个月降低高度0.80 mm (p = 0.067)，在3个月降低高度1.47 mm (p < 0.001)，在6个月降低高度2.00 mm (p < 0.001)，而i-PRF在相同的间隔降低高度1.20 mm (p = 0.003), 1.80 mm （p < 0.001）和2.27 mm （p < 0.001）。面积的减少也有类似的模式。各组间无显著差异。结论ha和i-PRF治疗黑三角是一种有效、微创、生物相容性好的治疗方法。HA提供了易用性和患者舒适度，而i-PRF是生物驱动的，具有成本效益。材料的选择应根据临床情况和患者的偏好。
{"title":"Comparative evaluation of Hyaluronic acid and injectable platelet-rich fibrin as a non-surgical approach for management of black triangle","authors":"Mansi Pal, Nand Lal, Umesh Pratap Verma, Anjani Kumar Pathak","doi":"10.1016/j.jobcr.2025.09.030","DOIUrl":"10.1016/j.jobcr.2025.09.030","url":null,"abstract":"<div><h3>Aims</h3><div>To compare and evaluate the clinical efficacy of Hyaluronic Acid and Injectable Platelet Rich Fibrin (i-PRF) in the management of gingival black triangles using clinical and photographic analysis over 6 months.</div></div><div><h3>Material and method</h3><div>Thirty patients (15 in each group) were evaluated in this comparative study. Patients were divided into two groups: <u>Group(HA) and Group(i-PRF).</u> Injections were administered at baseline, 3 weeks, and 6 weeks. Black triangle height and area were measured at baseline, 1 month, 3 months, and 6 months using a UNC-15 probe and ImageJ software. Data were analyzed using repeated measures ANOVA and Tukey's post hoc test.</div></div><div><h3>Results</h3><div>Both the HA and i-PRF groups showed significant reductions in black triangle height and area over a 6-month period. HA reduced height by 0.80 mm at 1 month (p = 0.067), 1.47 mm at 3 months (p < 0.001), and 2.00 mm at 6 months (p < 0.001), while i-PRF reduced height by 1.20 mm (p = 0.003), 1.80 mm (p < 0.001), and 2.27 mm (p < 0.001) at the same intervals. Area reductions followed similar patterns. No significant intergroup differences were observed.</div></div><div><h3>Conclusion</h3><div>HA and i-PRF are effective, minimally invasive, and biocompatible treatments for managing black triangles. HA offers ease of use and patient comfort, while i-PRF is biologically driven and cost-effective. Material selection should be based on clinical conditions and patient preferences.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1660-1667"},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-08DOI: 10.1016/j.jobcr.2025.09.027
Turaga Sai Susmitha, Jagadeeswara Rao Sukhabhogi, Dolar Doshi
Background
Sense of Coherence (SOC) is a psychological aspect that could have a significant effect on an individual's perception of aesthetics.
Aim
To assess the influence of Sense of Coherence on self-perceived dental appearance against normative orthodontic treatment need among 12 year old children.
Methods
An observational study was conducted among 268 school children aged 12 years old from in and around Hyderabad city to assess individuals’ Sense of Coherence (SOC-13), self-perceived aesthetics (Oral Aesthetic Subjective Impact Scale – 5 item) and malocclusion using Dental Aesthetic Index. Frequency distributions were used to describe the responses item wise for SOC and OASIS questionnaire. The difference between mean SOC scores between the groups was assessed using ANOVA. Multiple linear regression was performed to assess the affect of gender and OASIS on SOC.
Results
Nearly half of the sample (48.13 %) demonstrated higher Sense of Coherence and 58.96 % of individuals had positive perception of aesthetics. Remarkable percentage (78.73 %) of children had normative orthodontic treatment need. Highest mean SOC score (60.70 ± 9.78) was noted for those without orthodontic treatment need and negative aesthetic perception (p = 0.40). Self perceived dental aesthetics revealed a negative correlation with SOC (r = −0.02; p = 0.72). Multiple linear regression analysis revealed that SOC could be predicted only by gender (p = 0.01) but not self perceived aesthetics (p = 0.27)
Conclusion
In this study, Sense of Coherence did not influence the self perceived aesthetics irrespective of normative need.
{"title":"Sense of coherence and its relation to self perceived dental appearance and normative orthodontic treatment need among 12-year-old children","authors":"Turaga Sai Susmitha, Jagadeeswara Rao Sukhabhogi, Dolar Doshi","doi":"10.1016/j.jobcr.2025.09.027","DOIUrl":"10.1016/j.jobcr.2025.09.027","url":null,"abstract":"<div><h3>Background</h3><div>Sense of Coherence (SOC) is a psychological aspect that could have a significant effect on an individual's perception of aesthetics.</div></div><div><h3>Aim</h3><div>To assess the influence of Sense of Coherence on self-perceived dental appearance against normative orthodontic treatment need among 12 year old children.</div></div><div><h3>Methods</h3><div>An observational study was conducted among 268 school children aged 12 years old from in and around Hyderabad city to assess individuals’ Sense of Coherence (SOC-13), self-perceived aesthetics (Oral Aesthetic Subjective Impact Scale – 5 item) and malocclusion using Dental Aesthetic Index. Frequency distributions were used to describe the responses item wise for SOC and OASIS questionnaire. The difference between mean SOC scores between the groups was assessed using ANOVA. Multiple linear regression was performed to assess the affect of gender and OASIS on SOC.</div></div><div><h3>Results</h3><div>Nearly half of the sample (48.13 %) demonstrated higher Sense of Coherence and 58.96 % of individuals had positive perception of aesthetics. Remarkable percentage (78.73 %) of children had normative orthodontic treatment need. Highest mean SOC score (60.70 ± 9.78) was noted for those without orthodontic treatment need and negative aesthetic perception (p = 0.40). Self perceived dental aesthetics revealed a negative correlation with SOC (r = −0.02; p = 0.72). Multiple linear regression analysis revealed that SOC could be predicted only by gender (p = 0.01) but not self perceived aesthetics (p = 0.27)</div></div><div><h3>Conclusion</h3><div>In this study, Sense of Coherence did not influence the self perceived aesthetics irrespective of normative need.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1655-1659"},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-04DOI: 10.1016/j.jobcr.2025.09.025
M. Madhumitha , Devika S. Pillai , Pradeep Kumar Yadalam , Prasanthi Sitaraman
Background
Artificial Intelligence (AI) significantly enhances the diagnosis of pericoronal radiolucency by accurately interpreting dental radiographs. Through advanced algorithms, AI can identify early signs of abnormalities near unerupted teeth. This helps clinicians differentiate between benign and malignant conditions, leading to more informed decisions; improved treatment plans, ultimately benefiting patient care and outcomes.
Method
ology: A total of 2500 radiographs were screened of which 1070 radiographs were used in the study. 315 images of pericoronal radiolucency in mandibular third molars and 755 images of the normal mandibular third molars were included. The AI algorithms employed in the study were Logistic regression and Naive Bayes. Accuracy, sensitivity, specificity, precision, recall, F1, AUC-ROC curve were used for performance evaluation.
Results
This study found that Logistic regression model showed slightly higher accuracy than Naive Bayes model in predicting peri coronal radiolucency. In performance prediction for logistic regression model in predicting pericoronal radiolucency in third molars in 315 images, showed a slightly higher rate of prediction of 58.3 %, whereas, Naive Bayes model showed a comparatively lower prediction of pericoronal radiolucency, 52.2 %. During performance evaluation, Logistic regression performed better in CA, F1, and Recall, and Naive Bayes performed better in AUC and Precision model.
Conclusion
The current study demonstrated that Logistic regression have slightly highest accuracy in detecting pericoronal radiolucency in digital orthopantomogram images, which is consistent with the normal radiographic evaluation. Also, the Naive Bayes algorithm showed a fairly considerable performance in the classification of pericoronal radiolucencies.
{"title":"Role of artificial intelligence in diagnosing pericoronal radiolucency","authors":"M. Madhumitha , Devika S. Pillai , Pradeep Kumar Yadalam , Prasanthi Sitaraman","doi":"10.1016/j.jobcr.2025.09.025","DOIUrl":"10.1016/j.jobcr.2025.09.025","url":null,"abstract":"<div><h3>Background</h3><div>Artificial Intelligence (AI) significantly enhances the diagnosis of pericoronal radiolucency by accurately interpreting dental radiographs. Through advanced algorithms, AI can identify early signs of abnormalities near unerupted teeth. This helps clinicians differentiate between benign and malignant conditions, leading to more informed decisions; improved treatment plans, ultimately benefiting patient care and outcomes.</div></div><div><h3>Method</h3><div>ology: A total of 2500 radiographs were screened of which 1070 radiographs were used in the study. 315 images of pericoronal radiolucency in mandibular third molars and 755 images of the normal mandibular third molars were included. The AI algorithms employed in the study were Logistic regression and Naive Bayes. Accuracy, sensitivity, specificity, precision, recall, F1, AUC-ROC curve were used for performance evaluation.</div></div><div><h3>Results</h3><div>This study found that Logistic regression model showed slightly higher accuracy than Naive Bayes model in predicting peri coronal radiolucency. In performance prediction for logistic regression model in predicting pericoronal radiolucency in third molars in 315 images, showed a slightly higher rate of prediction of 58.3 %, whereas, Naive Bayes model showed a comparatively lower prediction of pericoronal radiolucency, 52.2 %. During performance evaluation, Logistic regression performed better in CA, F1, and Recall, and Naive Bayes performed better in AUC and Precision model.</div></div><div><h3>Conclusion</h3><div>The current study demonstrated that Logistic regression have slightly highest accuracy in detecting pericoronal radiolucency in digital orthopantomogram images, which is consistent with the normal radiographic evaluation. Also, the Naive Bayes algorithm showed a fairly considerable performance in the classification of pericoronal radiolucencies.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1648-1654"},"PeriodicalIF":0.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-02DOI: 10.1016/j.jobcr.2025.09.023
Maha El Shahawy
Objective
demonstrate the possible palliative role of curcumin in the prospective molecular and histological alterations in the sublingual glands of diabetic male rats.
Methods
Twenty-one male adult rats were used and randomly assigned into three groups (n = 7). The control group consisted of rats administered a single IP injection of saline. The diabetic group included rats receiving one dose of alloxan (140 mg/kg). The nanocurcumin-treated rat group (NC group) contained diabetic rats administered 200 mg/kg nanocurcumin. After 42 days, the salivary glands were dissected and assessed for Masson trichrome, Hematoxylin and eosin (H&E) staining, expression of c-kit and β-catenin, and histomorphometric analysis.
Results
The sublingual glands of diabetic rats revealed altered histology. In addition, the salivary tissues depicted an apparent change in β-catenin, and c-kit expression. The NC group resumed the normal sublingual gland architecture and c-kit and β-catenin expression.
Conclusion
Curcumin treatment rescued the histological and molecular changes in the sublingual glands of the diabetic rats. The ameliorating effect of curcumin on sublingual tissues of diabetic rats may be due to resuming of expression of β-catenin suggesting restoration of Wnt/β-catenin signaling in the glandular tissues which can maintain the c-kit + glandular stem/progenitor cells.
{"title":"Curcumin mitigates the histopathological and molecular changes in sublingual glands in diabetic male rats","authors":"Maha El Shahawy","doi":"10.1016/j.jobcr.2025.09.023","DOIUrl":"10.1016/j.jobcr.2025.09.023","url":null,"abstract":"<div><h3>Objective</h3><div>demonstrate the possible palliative role of curcumin in the prospective molecular and histological alterations in the sublingual glands of diabetic male rats.</div></div><div><h3>Methods</h3><div>Twenty-one male adult rats were used and randomly assigned into three groups (n = 7). The control group consisted of rats administered a single IP injection of saline. The diabetic group included rats receiving one dose of alloxan (140 mg/kg). The nanocurcumin-treated rat group (NC group) contained diabetic rats administered 200 mg/kg nanocurcumin. After 42 days, the salivary glands were dissected and assessed for Masson trichrome, Hematoxylin and eosin (H&E) staining, expression of c-kit and β-catenin, and histomorphometric analysis.</div></div><div><h3>Results</h3><div>The sublingual glands of diabetic rats revealed altered histology. In addition, the salivary tissues depicted an apparent change in β-catenin, and c-kit expression. The NC group resumed the normal sublingual gland architecture and c-kit and β-catenin expression.</div></div><div><h3>Conclusion</h3><div>Curcumin treatment rescued the histological and molecular changes in the sublingual glands of the diabetic rats. The ameliorating effect of curcumin on sublingual tissues of diabetic rats may be due to resuming of expression of β-catenin suggesting restoration of Wnt/β-catenin signaling in the glandular tissues which can maintain the c-kit + glandular stem/progenitor cells.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1641-1647"},"PeriodicalIF":0.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Healing abutments are subjected to multiple cycles of reuse owing to the limited wear and tear experienced in function. The study undertaken focuses on drawing a comparative evaluation amongst two decontamination protocols for reusing healing abutments.
Materials and method
A total of 85 healing abutments were divided into 3 groups. Group 1 contained 5 unused healing abutments. Group 2 contained 40 used healing abutments which were subjected to ultrasonic cleaning followed by autoclaving. Group 3 also contained 40 used healing abutments which were subjected to decontamination using NaOCl followed by ultrasonic cleaning followed by autoclaving. Healing abutments from all 3 groups were then individually stained with Phloxine B stain for a duration of 1 min and were individually observed under 10X Stereomicroscope for traces of staining which indicated residual contamination.
Statistical analysis
ANOVA test and chi-square test were employed to statistically analyze the 3 groups using SPSS software.
Result
The difference between the 3 groups was statistically significant (p < 0.001) with a chi-Square value of 85 %. Intergroup Comparison of mean rank showed statistically significant difference between the groups (Kruskal Wallis ANOVA, p < 0.001). Groups 1 and 3 did not display any evidence of staining with Phloxine B indicating no residual contamination, whereas all samples belonging to Group 2 displayed biological remnants as evidenced by the presence of staining.
Conclusion
Cleaning of used healing abutments using Sodium hypochlorite followed by ultrasonic cleaning and autoclaving shows greater efficiency in ensuring complete decontamination of used healing abutments as compared to ultrasonic cleaning and autoclaving.
目的修复基牙由于其功能的有限磨损，需要经历多次重复使用。所进行的研究侧重于在重复使用愈合基台的两种去污方案之间进行比较评估。材料与方法85个愈合基台分为3组。组1包含5个未使用的愈合基牙。第2组40个使用过的愈合基牙，超声清洗后进行高压灭菌。第三组40个使用过的愈合基牙，用NaOCl去污，然后超声清洗，然后高压灭菌。3组愈合基牙分别用Phloxine B染色1 min，在10倍体视显微镜下观察是否有残留污染的染色痕迹。统计学分析采用方差分析和卡方检验，采用SPSS软件对3组进行统计学分析。结果3组间差异有统计学意义（p < 0.001），卡方值为85%。组间平均秩比较显示组间差异有统计学意义（Kruskal Wallis ANOVA, p < 0.001）。第1组和第3组没有显示出任何污染残留的证据，而第2组的所有样品都显示出生物残留，这表明存在染色。结论次氯酸钠清洗后超声清洗和高压灭菌比超声清洗和高压灭菌能更有效地保证愈合基牙的完全去污。
{"title":"Comparative evaluation of the efficiency of different decontamination protocols for reusing healing abutments: An in vitro study","authors":"Dhruv Patel, Nayana Shriram Anasane, V.N.V. Madhav","doi":"10.1016/j.jobcr.2025.09.024","DOIUrl":"10.1016/j.jobcr.2025.09.024","url":null,"abstract":"<div><h3>Purpose</h3><div>Healing abutments are subjected to multiple cycles of reuse owing to the limited wear and tear experienced in function. The study undertaken focuses on drawing a comparative evaluation amongst two decontamination protocols for reusing healing abutments.</div></div><div><h3>Materials and method</h3><div>A total of 85 healing abutments were divided into 3 groups. Group 1 contained 5 unused healing abutments. Group 2 contained 40 used healing abutments which were subjected to ultrasonic cleaning followed by autoclaving. Group 3 also contained 40 used healing abutments which were subjected to decontamination using NaOCl followed by ultrasonic cleaning followed by autoclaving. Healing abutments from all 3 groups were then individually stained with Phloxine B stain for a duration of 1 min and were individually observed under 10X Stereomicroscope for traces of staining which indicated residual contamination.</div></div><div><h3>Statistical analysis</h3><div>ANOVA test and chi-square test were employed to statistically analyze the 3 groups using SPSS software.</div></div><div><h3>Result</h3><div>The difference between the 3 groups was statistically significant (p < 0.001) with a chi-Square value of 85 %. Intergroup Comparison of mean rank showed statistically significant difference between the groups (Kruskal Wallis ANOVA, p < 0.001). Groups 1 and 3 did not display any evidence of staining with Phloxine B indicating no residual contamination, whereas all samples belonging to Group 2 displayed biological remnants as evidenced by the presence of staining.</div></div><div><h3>Conclusion</h3><div>Cleaning of used healing abutments using Sodium hypochlorite followed by ultrasonic cleaning and autoclaving shows greater efficiency in ensuring complete decontamination of used healing abutments as compared to ultrasonic cleaning and autoclaving.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1636-1640"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There is an ambiguity in the correct diagnosis of odontogenic cystic lesions in mixed dentition period. So, present study was planned to assess diagnostic discrepancies and their potential impact on treatment strategies in pediatric odontogenic cysts.
Material method
The data of the patients was retrieved from the digital records of patients from January 2014 to January 2024. After screening of the digital records, 61 cases were selected for screening, for demographic details, various clinical characteristics, radiographic investigations (OPG, CBCT etc.). For the calculation of the discrepancy between clinical and histopathological diagnosis of the radicular cyst, dentigerous cyst, and odontogenic kerato-cyst the Discrepancy Index was calculated.
Results
The results revealed that 61 cases of various cystic conditions were identified. Among them, the dentigerous cyst constituted 14.7 % (9cases), radicular cyst constituted 42.6 % (26cases), and Odontogenic kerato-cyst constitutes 27.86 % (17 cases) with the mean age (in years) of reporting 9.55 ± 3.16, 9.00 ± 2.79, and10.06 ± 2.43 respectively. The odontogenic cysts were commonly found in mandibular posterior region. In patients with dentigerous cysts, 44.44 % had a history of extraction of primary teeth, 55.55 % had decayed/pulpectomised teeth. Among them the maximum discrepancy index was observed between dentigerous cysts and Odontogenic kerato-cysts i.e., 50 %, followed by radicular cyst and dentigerous cyst or vice-versa (21.42 %).
Conclusion
Despite the difficult diagnosis of dentigerous cyst, radicular cyst and OKCs in mixed dentition, cystic lesions should be examined thoroughly and diagnosed carefully. Misinterpreting a cyst as a tumor, could lead to aggressive surgical intervention when a less invasive approach would suffice.
{"title":"Discrepancy in diagnosis and characteristics of odontogenic cystic lesions in mixed dentition period; a retrospective study","authors":"Umer Mukhtar, Rigzen Tamchos, Manoj kumar Jaiswal, Sadia Iqbal","doi":"10.1016/j.jobcr.2025.09.028","DOIUrl":"10.1016/j.jobcr.2025.09.028","url":null,"abstract":"<div><h3>Background</h3><div>There is an ambiguity in the correct diagnosis of odontogenic cystic lesions in mixed dentition period. So, present study was planned to assess diagnostic discrepancies and their potential impact on treatment strategies in pediatric odontogenic cysts.</div></div><div><h3>Material method</h3><div>The data of the patients was retrieved from the digital records of patients from January 2014 to January 2024. After screening of the digital records, 61 cases were selected for screening, for demographic details, various clinical characteristics, radiographic investigations (OPG, CBCT etc.). For the calculation of the discrepancy between clinical and histopathological diagnosis of the radicular cyst, dentigerous cyst, and odontogenic kerato-cyst the Discrepancy Index was calculated.</div></div><div><h3>Results</h3><div>The results revealed that 61 cases of various cystic conditions were identified. Among them, the dentigerous cyst constituted 14.7 % (9cases), radicular cyst constituted 42.6 % (26cases), and Odontogenic kerato-cyst constitutes 27.86 % (17 cases) with the mean age (in years) of reporting 9.55 ± 3.16, 9.00 ± 2.79, and10.06 ± 2.43 respectively. The odontogenic cysts were commonly found in mandibular posterior region. In patients with dentigerous cysts, 44.44 % had a history of extraction of primary teeth, 55.55 % had decayed/pulpectomised teeth. Among them the maximum discrepancy index was observed between dentigerous cysts and Odontogenic kerato-cysts i.e., 50 %, followed by radicular cyst and dentigerous cyst or vice-versa (21.42 %).</div></div><div><h3>Conclusion</h3><div>Despite the difficult diagnosis of dentigerous cyst, radicular cyst and OKCs in mixed dentition, cystic lesions should be examined thoroughly and diagnosed carefully. Misinterpreting a cyst as a tumor, could lead to aggressive surgical intervention when a less invasive approach would suffice.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1628-1635"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-27DOI: 10.1016/j.jobcr.2025.09.022
Praneeth rednam , Sarath sarathy
Aim
To assess MMP 2 and MMP 9 levels in pulpal blood of human molar teeth with symptomatic irreversible pulpitis in 2 different age groups.
Materials and methods
Patients of two age groups young [18–40] and old [>60] with symptomatic irreversible pulpitis in molars were included in this study. Teeth were isolated using a rubber dam. The rubber dam, clamp, and tooth were decontaminated using a cotton swab drenched in 1 % NaOCl solution. Access cavities were then prepared using a diamond-coated bur in a contrangle handpiece under water-cooling. Subsequently, the caries was excavated using a sterile round bur. Pulpal blood was collected from the pulp chamber by placing the blunt end of a sterile inverted 60 size paper point for 1 min. The paper point was transferred into a sterile microcentrifugation tube and frozen at −20 °C immediately until further processing. The levels of MMP-9 and MMP-2 in these samples were measured using commercially available specific enzyme-linked immunosorbent assay (ELISA) kits. Data was analysed using an independent t-test.
Results
The mean pulpal blood levels of MMP-9 in patients above 60 years (220 ng/ml) were significantly lower than those aged 18–40 years (468 ng/ml) ([p value < 0.05]
The mean pulpal blood levels of MMP-2 in patients above 60 years (134 ng/ml) were significantly lower than in those aged 18–40 years (153 ng/ml [p value < 0.05].
Conclusion
MMP-9 and MMP-2 levels in pulpal blood of cariously exposed teeth with irreversible pulpitis can vary with age.
{"title":"Evaluation of the role of age in MMP 9 and MMP 2 levels in pulpal blood of teeth with symptomatic irreversible pulpitis","authors":"Praneeth rednam , Sarath sarathy","doi":"10.1016/j.jobcr.2025.09.022","DOIUrl":"10.1016/j.jobcr.2025.09.022","url":null,"abstract":"<div><h3>Aim</h3><div>To assess MMP 2 and MMP 9 levels in pulpal blood of human molar teeth with symptomatic irreversible pulpitis in 2 different age groups.</div></div><div><h3>Materials and methods</h3><div>Patients of two age groups young [18–40] and old [>60] with symptomatic irreversible pulpitis in molars were included in this study. Teeth were isolated using a rubber dam. The rubber dam, clamp, and tooth were decontaminated using a cotton swab drenched in 1 % NaOCl solution. Access cavities were then prepared using a diamond-coated bur in a contrangle handpiece under water-cooling. Subsequently, the caries was excavated using a sterile round bur. Pulpal blood was collected from the pulp chamber by placing the blunt end of a sterile inverted 60 size paper point for 1 min. The paper point was transferred into a sterile microcentrifugation tube and frozen at −20 °C immediately until further processing. The levels of MMP-9 and MMP-2 in these samples were measured using commercially available specific enzyme-linked immunosorbent assay (ELISA) kits. Data was analysed using an independent t-test.</div></div><div><h3>Results</h3><div>The mean pulpal blood levels of MMP-9 in patients above 60 years (220 ng/ml) were significantly lower than those aged 18–40 years (468 ng/ml) ([p value < 0.05]</div><div>The mean pulpal blood levels of MMP-2 in patients above 60 years (134 ng/ml) were significantly lower than in those aged 18–40 years (153 ng/ml [p value < 0.05].</div></div><div><h3>Conclusion</h3><div>MMP-9 and MMP-2 levels in pulpal blood of cariously exposed teeth with irreversible pulpitis can vary with age.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1624-1627"},"PeriodicalIF":0.0,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-24DOI: 10.1016/j.jobcr.2025.09.016
P.S. Tripthi, Rakshita Kumar, Padmaraj Hegde
Introduction
Postoperative pain following third molar extractions is a significant concern, often managed using opioids and NSAIDs, associated with considerable side effects. Lignocaine patches, known for their localized action and minimal systemic effects, have shown promise in managing various pain conditions. This study aimed to evaluate the efficacy of 5 % lignocaine patches in reducing postoperative pain following third molar extractions.
Methodology
A prospective, split-mouth study was conducted on 30 patients aged 18–40 years requiring bilateral extraction of impacted mandibular third molars. Post extraction on one side (test lignocaine patches were applied postoperatively, while post extraction of contralateral side, standard oral analgesics were given. Pain levels were assessed using the Visual Analog Scale (VAS) on the day of extraction, postoperative day (POD) 1 and POD 2. Additional patches or analgesics were provided if pain exceeded VAS thresholds.
Results
The test group reported significantly lower mean VAS scores (1.96 ± 0.72) compared to the control group (2.55 ± 0.35, p = 0.004). Significant reduction in pain was observed in both groups over time, but the test group showed greater pain relief by POD 2 (p = 0.000) and no major adverse effects were reported, only 13.3 % experienced inadequate analgesia, requiring the need of a second patch.
Conclusion
These findings suggest that 5 % lignocaine patches offer an effective alternative to oral analgesics for managing postoperative pain after third molar extractions. Further research, however, is recommended to explore their potential in reducing opioid use and their effectiveness in other minor oral surgical procedures.
{"title":"Assessment of efficacy of lignocaine patch in pain management post third molar extraction: A prospective, split mouth study","authors":"P.S. Tripthi, Rakshita Kumar, Padmaraj Hegde","doi":"10.1016/j.jobcr.2025.09.016","DOIUrl":"10.1016/j.jobcr.2025.09.016","url":null,"abstract":"<div><h3>Introduction</h3><div>Postoperative pain following third molar extractions is a significant concern, often managed using opioids and NSAIDs, associated with considerable side effects. Lignocaine patches, known for their localized action and minimal systemic effects, have shown promise in managing various pain conditions. This study aimed to evaluate the efficacy of 5 % lignocaine patches in reducing postoperative pain following third molar extractions.</div></div><div><h3>Methodology</h3><div>A prospective, split-mouth study was conducted on 30 patients aged 18–40 years requiring bilateral extraction of impacted mandibular third molars. Post extraction on one side (test lignocaine patches were applied postoperatively, while post extraction of contralateral side, standard oral analgesics were given. Pain levels were assessed using the Visual Analog Scale (VAS) on the day of extraction, postoperative day (POD) 1 and POD 2. Additional patches or analgesics were provided if pain exceeded VAS thresholds.</div></div><div><h3>Results</h3><div>The test group reported significantly lower mean VAS scores (1.96 ± 0.72) compared to the control group (2.55 ± 0.35, <em>p</em> = 0.004). Significant reduction in pain was observed in both groups over time, but the test group showed greater pain relief by POD 2 (<em>p</em> = 0.000) and no major adverse effects were reported, only 13.3 % experienced inadequate analgesia, requiring the need of a second patch.</div></div><div><h3>Conclusion</h3><div>These findings suggest that 5 % lignocaine patches offer an effective alternative to oral analgesics for managing postoperative pain after third molar extractions. Further research, however, is recommended to explore their potential in reducing opioid use and their effectiveness in other minor oral surgical procedures.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1601-1606"},"PeriodicalIF":0.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145117705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号