TRMT112 is a member of the transfer RNA (tRNA) methyltransferase family, and its dysregulation in humans is involved in carcinogenesis. This study aimed to investigate the expression and clinical significance of TRMT112 in patients with oral squamous cell carcinoma (OSCC).
Materials and methods
Quantitative real-time PCR (qPCR) and Western blot were used to analyze TRMT112 expression in paired tumor and non-tumor tissues of OSCC. Furthermore, we analyzed TRMT112 expression for clinicopathological features, prognosis, immune infiltration, and immunotherapy response using the TCGA-HNSCC datasets, which primarily include OSCC data through UALCAN, Human Protein Atlas, Kaplan-Meier plots, and TIMER2.0. The oncogenic role and mechanism of TRMT112 were analyzed using a functional enrichment approach.
Results
TRMT112 expression was significantly upregulated in OSCC tissues compared to non-tumor tissues. The upregulated expression of TRMT112 was associated with advanced tumor stages, metastasis, lower immune infiltrating levels, immunotherapy resistance, and worse prognosis. Protein network and functional pathway enrichment analysis revealed that TRMT112 interacts with well-known oncoproteins that play a critical role in oral cancer progression.
Conclusions
Overall, our novel findings revealed that TRMT112 is associated with the oncogenic process of OSCC, which suggests that TRMT112 could serve as a potential prognostic and therapeutic candidate.
trmt112是转移RNA （tRNA）甲基转移酶家族的一员，其在人类中的失调与致癌有关。本研究旨在探讨TRMT112在口腔鳞状细胞癌（OSCC）患者中的表达及临床意义。材料与方法采用实时荧光定量PCR （quantitative real-time PCR, qPCR）和Western blot技术分析TRMT112在OSCC配对肿瘤组织和非肿瘤组织中的表达。此外，我们使用TCGA-HNSCC数据集分析了TRMT112表达与临床病理特征、预后、免疫浸润和免疫治疗反应的关系，该数据集主要包括通过UALCAN、Human Protein Atlas、Kaplan-Meier图和TIMER2.0获得的OSCC数据。利用功能富集法分析了TRMT112的致癌作用和机制。结果与非肿瘤组织相比，strmt112在OSCC组织中的表达明显上调。TRMT112表达上调与肿瘤分期、转移、免疫浸润水平降低、免疫治疗耐药、预后差相关。蛋白网络和功能通路富集分析显示，TRMT112与已知的在口腔癌进展中起关键作用的癌蛋白相互作用。总之，我们的新发现揭示了TRMT112与OSCC的致癌过程有关，这表明TRMT112可能作为潜在的预后和治疗候选基因。
{"title":"High expression of TRMT112 is associated with the development of oral squamous cell carcinoma","authors":"Anitha Pandi , Premkumar Rajendhiran , Vijayashree Priyadharsini Jayaseelan , Paramasivam Arumugam","doi":"10.1016/j.jobcr.2025.12.014","DOIUrl":"10.1016/j.jobcr.2025.12.014","url":null,"abstract":"<div><h3>Background</h3><div>TRMT112 is a member of the transfer RNA (tRNA) methyltransferase family, and its dysregulation in humans is involved in carcinogenesis. This study aimed to investigate the expression and clinical significance of TRMT112 in patients with oral squamous cell carcinoma (OSCC).</div></div><div><h3>Materials and methods</h3><div>Quantitative real-time PCR (qPCR) and Western blot were used to analyze TRMT112 expression in paired tumor and non-tumor tissues of OSCC. Furthermore, we analyzed TRMT112 expression for clinicopathological features, prognosis, immune infiltration, and immunotherapy response using the TCGA-HNSCC datasets, which primarily include OSCC data through UALCAN, Human Protein Atlas, Kaplan-Meier plots, and TIMER2.0. The oncogenic role and mechanism of TRMT112 were analyzed using a functional enrichment approach.</div></div><div><h3>Results</h3><div>TRMT112 expression was significantly upregulated in OSCC tissues compared to non-tumor tissues. The upregulated expression of TRMT112 was associated with advanced tumor stages, metastasis, lower immune infiltrating levels, immunotherapy resistance, and worse prognosis. Protein network and functional pathway enrichment analysis revealed that TRMT112 interacts with well-known oncoproteins that play a critical role in oral cancer progression.</div></div><div><h3>Conclusions</h3><div>Overall, our novel findings revealed that TRMT112 is associated with the oncogenic process of OSCC, which suggests that TRMT112 could serve as a potential prognostic and therapeutic candidate.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 273-278"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adequate polymerization of composite resin restorations is critical for their mechanical performance and long-term clinical success. Salivary contamination during light curing can interfere with polymerization and may influence color stability.
Objective
This in vitro study examined how artificial saliva contamination at specific intervals during light curing affects the DC (degree of conversion) and short-term color change or ΔE (Delta E) of two composite resins—Gradia Direct (microhybrid) and N-Ceram Bulk Fill (nanohybrid).
Methods
One hundred eighty disk specimens (n = 90 per composite) were allocated into nine experimental groups differing in contamination timing and curing duration. DC was determined by FTIR-ATR (Fourier-Transform Infrared- Attenuated Total Reflectance) spectroscopy immediately after curing and following 24-h incubation at 37 °C. Color change (ΔE) was measured with a spectrophotometer using the CIE (International Commission on Illumination) Lab∗ system. Statistical analysis employed one- and two-way ANOVA (Analysis of Variance) with Tukey's post hoc test (α = 0.05).
Results
Early contamination (within the first 5–10 s) produced a significant reduction in DC for both materials (p < 0.001), with N-Ceram showing the greatest loss. Gradia achieved higher DC across most conditions. Post-cure incubation improved DC in all groups, most notably in N-Ceram. All ΔE values remained below the perceptibility threshold of 3.3, with no significant differences among groups (p > 0.05).
Conclusion
Saliva exposure early in light curing markedly reduces polymerization efficiency, although extended curing and post-cure polymerization can partially restore DC. Short-term color stability appears unaffected. Strict field isolation and optimized curing protocols are essential to maximize clinical performance.
{"title":"The influence of salivary contamination during light curing on degree of conversion and color stability of two composite resins","authors":"Marzieh Rohaninasab , Shima Falahat , Golnaz Tayebi , Farzaneh Manouchehri , Farzaneh Sadeghi Mahounak","doi":"10.1016/j.jobcr.2025.12.009","DOIUrl":"10.1016/j.jobcr.2025.12.009","url":null,"abstract":"<div><h3>Background</h3><div>Adequate polymerization of composite resin restorations is critical for their mechanical performance and long-term clinical success. Salivary contamination during light curing can interfere with polymerization and may influence color stability.</div></div><div><h3>Objective</h3><div>This in vitro study examined how artificial saliva contamination at specific intervals during light curing affects the DC (degree of conversion) and short-term color change or ΔE (Delta E) of two composite resins—Gradia Direct (microhybrid) and N-Ceram Bulk Fill (nanohybrid).</div></div><div><h3>Methods</h3><div>One hundred eighty disk specimens (n = 90 per composite) were allocated into nine experimental groups differing in contamination timing and curing duration. DC was determined by FTIR-ATR (Fourier-Transform Infrared- Attenuated Total Reflectance) spectroscopy immediately after curing and following 24-h incubation at 37 °C. Color change (ΔE) was measured with a spectrophotometer using the CIE (International Commission on Illumination) L<em>a</em>b∗ system. Statistical analysis employed one- and two-way ANOVA (Analysis of Variance) with Tukey's post hoc test (α = 0.05).</div></div><div><h3>Results</h3><div>Early contamination (within the first 5–10 s) produced a significant reduction in DC for both materials (p < 0.001), with N-Ceram showing the greatest loss. Gradia achieved higher DC across most conditions. Post-cure incubation improved DC in all groups, most notably in N-Ceram. All ΔE values remained below the perceptibility threshold of 3.3, with no significant differences among groups (p > 0.05).</div></div><div><h3>Conclusion</h3><div>Saliva exposure early in light curing markedly reduces polymerization efficiency, although extended curing and post-cure polymerization can partially restore DC. Short-term color stability appears unaffected. Strict field isolation and optimized curing protocols are essential to maximize clinical performance.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 254-259"},"PeriodicalIF":0.0,"publicationDate":"2025-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-17DOI: 10.1016/j.jobcr.2025.12.010
Vazeeha Afrin Syed, Arvina Rajasekar
Objectives
Coronally advanced flap (CAF) combined with connective tissue graft (CTG) is a gold standard for root coverage but is limited by donor site morbidity. The aim of this study was to compare the clinical, patient-centered, and molecular outcomes of CAF combined with xenogeneic collagen matrix (XCM) versus CTG in the treatment of maxillary gingival recession defects, with a focus on COL1A1 gene expression.
Methods
This randomized controlled trial included 40 patients with Cairo RT1 gingival recession defects, allocated into two groups: CAF + XCM (test; n = 20) and CAF + CTG (control; n = 20). Clinical parameters including probing pocket depth (PPD), clinical attachment level (CAL), width of keratinized tissue (WKT), recession height (RH), recession width (RW), and mean root coverage (MRC) were evaluated at baseline and 6 months. Root sensitivity was assessed at baseline and 6 months, while postoperative pain was recorded at 24 h, 7 days, and 14 days. Surgical duration was measured, and COL1A1 gene expression in gingival crevicular fluid was quantified using real-time quantitative PCR.
Results
Both groups showed significant clinical improvement and upregulation of COL1A1 expression at 6 months (p < 0.05). Intergroup differences in clinical and molecular outcomes were not statistically significant. However, the XCM group had significantly shorter surgical time and lower postoperative pain scores (p < 0.05), indicating improved patient comfort.
Conclusions
XCM offers comparable clinical and molecular outcomes to CTG with the added benefits of reduced surgical time and morbidity, making it a viable, patient-friendly alternative in the management of gingival recession.
{"title":"Comparative analysis of clinical, patient-centered, and COL1A1 gene expression outcomes following coronally advanced flap with xenogeneic collagen matrix versus connective tissue graft in maxillary gingival recession: A parallel-arm, single-blinded randomized controlled clinical trial","authors":"Vazeeha Afrin Syed, Arvina Rajasekar","doi":"10.1016/j.jobcr.2025.12.010","DOIUrl":"10.1016/j.jobcr.2025.12.010","url":null,"abstract":"<div><h3>Objectives</h3><div>Coronally advanced flap (CAF) combined with connective tissue graft (CTG) is a gold standard for root coverage but is limited by donor site morbidity. The aim of this study was to compare the clinical, patient-centered, and molecular outcomes of CAF combined with xenogeneic collagen matrix (XCM) versus CTG in the treatment of maxillary gingival recession defects, with a focus on COL1A1 gene expression.</div></div><div><h3>Methods</h3><div>This randomized controlled trial included 40 patients with Cairo RT1 gingival recession defects, allocated into two groups: CAF + XCM (test; n = 20) and CAF + CTG (control; n = 20). Clinical parameters including probing pocket depth (PPD), clinical attachment level (CAL), width of keratinized tissue (WKT), recession height (RH), recession width (RW), and mean root coverage (MRC) were evaluated at baseline and 6 months. Root sensitivity was assessed at baseline and 6 months, while postoperative pain was recorded at 24 h, 7 days, and 14 days. Surgical duration was measured, and COL1A1 gene expression in gingival crevicular fluid was quantified using real-time quantitative PCR.</div></div><div><h3>Results</h3><div>Both groups showed significant clinical improvement and upregulation of COL1A1 expression at 6 months (p < 0.05). Intergroup differences in clinical and molecular outcomes were not statistically significant. However, the XCM group had significantly shorter surgical time and lower postoperative pain scores (p < 0.05), indicating improved patient comfort.</div></div><div><h3>Conclusions</h3><div>XCM offers comparable clinical and molecular outcomes to CTG with the added benefits of reduced surgical time and morbidity, making it a viable, patient-friendly alternative in the management of gingival recession.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 246-253"},"PeriodicalIF":0.0,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-16DOI: 10.1016/j.jobcr.2025.12.005
Kuldeep Singh, Priya Kumar, Aadithya B. Urs
Background
Multinucleated Giant Cells (MGCs) are akin to immune cells that play a crucial role in the tumor microenvironment. The present study aimed to evaluate the prognostic and clinicopathological significance of MGCs in Oral Squamous Cell Carcinoma (OSCC) through comprehensive analysis of their presence, localization, and correlation with survival outcomes.
Objective
To determine whether the presence and distribution of MGCs in OSCC correlates with tumor stage, histopathological features, and overall survival, thereby assessing their potential prognostic value.
Material and methods
100 proven cases of OSCC were retrieved with complete clinicopathological data. Haematoxylin-eosin sections were reviewed independently by three pathologists and grouped into two, with and without MGCs. The distribution, and average number of MGCs per 5HPF per case were examined. All clinicopathological parameters and prognosis based on Bryne's grading were compared between groups. The overall survival with follow-up period of >36 months was analyzed.
Results
34 cases with MGCs were found, out of which 21 patients are alive. Cases with MGCs had pTNM stage III whereas majority of cases without MGC's had stage IV. Significant correlation was found for lympho-vascular invasion only. Cases with MGC's showed good prognostic score according to Bryne's grading, while without MGC's showed moderate to poor prognostic score. Cases of alveolar mucosa, including gingiva and ridge SCC with MGCs, showed poor survival.
Conclusions
This study highlights the potential role of MGCs in OSCC, suggesting an association with earlier tumor stages and favorable prognostic scores. Further research is needed to assess their prognostic and therapeutic relevance.
{"title":"Prognostic significance of multinucleated giant cells in oral squamous cell carcinoma: A retrospective clinicopathological study","authors":"Kuldeep Singh, Priya Kumar, Aadithya B. Urs","doi":"10.1016/j.jobcr.2025.12.005","DOIUrl":"10.1016/j.jobcr.2025.12.005","url":null,"abstract":"<div><h3>Background</h3><div>Multinucleated Giant Cells (MGCs) are akin to immune cells that play a crucial role in the tumor microenvironment. The present study aimed to evaluate the prognostic and clinicopathological significance of MGCs in Oral Squamous Cell Carcinoma (OSCC) through comprehensive analysis of their presence, localization, and correlation with survival outcomes.</div></div><div><h3>Objective</h3><div>To determine whether the presence and distribution of MGCs in OSCC correlates with tumor stage, histopathological features, and overall survival, thereby assessing their potential prognostic value.</div></div><div><h3>Material and methods</h3><div>100 proven cases of OSCC were retrieved with complete clinicopathological data. Haematoxylin-eosin sections were reviewed independently by three pathologists and grouped into two, with and without MGCs. The distribution, and average number of MGCs per 5HPF per case were examined. All clinicopathological parameters and prognosis based on Bryne's grading were compared between groups. The overall survival with follow-up period of >36 months was analyzed.</div></div><div><h3>Results</h3><div>34 cases with MGCs were found, out of which 21 patients are alive. Cases with MGCs had pTNM stage III whereas majority of cases without MGC's had stage IV. Significant correlation was found for lympho-vascular invasion only. Cases with MGC's showed good prognostic score according to Bryne's grading, while without MGC's showed moderate to poor prognostic score. Cases of alveolar mucosa, including gingiva and ridge SCC with MGCs, showed poor survival.</div></div><div><h3>Conclusions</h3><div>This study highlights the potential role of MGCs in OSCC, suggesting an association with earlier tumor stages and favorable prognostic scores. Further research is needed to assess their prognostic and therapeutic relevance.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 240-245"},"PeriodicalIF":0.0,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The goal of periodontal treatment is to halt disease progression and restore the structure and function of damaged periodontal tissues. Homeodomains—transcription factors prominently expressed during limb bud formation and craniofacial development—are abundantly present in the periosteum, and their expression continues in postnatal descendant cells, where they play a key role in bone homeostasis and fracture healing. These factors regulate stem cell differentiation critical for cementogenesis, osteogenesis, and periodontal ligament (PDL) regeneration, making them attractive targets for periodontal tissue engineering and regenerative therapies. A deeper understanding of homeodomain protein functions could lead to innovative treatments for periodontal disease and alveolar bone defects. In this context, our study investigated the role of homeodomain proteins in periodontal regeneration.
Methods
Periosteum tissue was collected from a fracture site, and protein extracts were prepared. MTT and in vitro wound-healing assays were performed using a PDL cell line co-cultured with homeodomain protein (test group) and PDL cells alone (control group).
Results
The MTT assay revealed that a concentration of 5 μg/mL yielded the highest cell viability. In the wound-healing assay, significant differences between the control and test groups demonstrated that homeodomain expression enhances cell migration and proliferation.
Conclusion
Homeodomain protein expression in the periosteum could serve as a novel biomarker for periodontal regeneration.
{"title":"Evaluating the role of a novel homeodomain protein in periodontal regenerative therapy: An in-vitro study","authors":"Bhavya Shetty , Rohit Prasad , Amrutha Rao , Tanya Singh , Akshatha Raj , Safiya Fatima Khan","doi":"10.1016/j.jobcr.2025.12.004","DOIUrl":"10.1016/j.jobcr.2025.12.004","url":null,"abstract":"<div><h3>Introduction</h3><div>The goal of periodontal treatment is to halt disease progression and restore the structure and function of damaged periodontal tissues. Homeodomains—transcription factors prominently expressed during limb bud formation and craniofacial development—are abundantly present in the periosteum, and their expression continues in postnatal descendant cells, where they play a key role in bone homeostasis and fracture healing. These factors regulate stem cell differentiation critical for cementogenesis, osteogenesis, and periodontal ligament (PDL) regeneration, making them attractive targets for periodontal tissue engineering and regenerative therapies. A deeper understanding of homeodomain protein functions could lead to innovative treatments for periodontal disease and alveolar bone defects. In this context, our study investigated the role of homeodomain proteins in periodontal regeneration.</div></div><div><h3>Methods</h3><div>Periosteum tissue was collected from a fracture site, and protein extracts were prepared. MTT and in vitro wound-healing assays were performed using a PDL cell line co-cultured with homeodomain protein (test group) and PDL cells alone (control group).</div></div><div><h3>Results</h3><div>The MTT assay revealed that a concentration of 5 μg/mL yielded the highest cell viability. In the wound-healing assay, significant differences between the control and test groups demonstrated that homeodomain expression enhances cell migration and proliferation.</div></div><div><h3>Conclusion</h3><div>Homeodomain protein expression in the periosteum could serve as a novel biomarker for periodontal regeneration.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 222-225"},"PeriodicalIF":0.0,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dental hypersensitivity (DH) is a common issue characterized by discomfort and pain when cold, hot, or acidic substances are consumed. DH treatment aims to control or eliminate causative factors and utilize desensitizing agents to occlude open dentinal tubules; however, there is no standardized definitive treatment yet. The increasing use of lasers, especially diode lasers, along with innovative materials such as Biodentin has contributed to advancing treatments. This study aimed to evaluate and compare the efficacy of a diode laser and Biodentin in the treatment of dentinal tubule occlusion.
Materials & methods
In this experimental study, 48 extracted human teeth were randomly divided into four groups: a control group (no treatment), a Biodentine-treated group, a diode laser 660 nm-treated group, and a combination group treated with both laser and Biodentine. After treatment, the samples were examined by scanning electron microscopy (SEM), and the percentage of tubule occlusion was categorized into four levels.
Results
A total of 83.3 % of the samples in the combination group (laser and Biodentine) achieved level 3 occlusion (more than 75 % occlusion), whereas 91.7 % of those in the Biodentine-only group were also in level 3. The percentage of patients in the laser-only group was 33.3 % in Level 2 and 66.7 % in Level 1 occlusion.
Conclusion
In conclusion, there was no significant difference in the occlusion measurement between the combination treatment group and the Biodentine-only group, with both being primarily rated as Level 3 occlusions. Therefore, the use of a diode laser at 660 nm in conjunction with Biodentine has effects similar to those of Biodentine alone.
{"title":"Evaluation and comparison of the efficacy of the diode laser and Biodentine on dentinal tubule occlusion","authors":"Zahra Jalalian , Fatemeh Dibaji , Marzieh Rohaninasab","doi":"10.1016/j.jobcr.2025.11.014","DOIUrl":"10.1016/j.jobcr.2025.11.014","url":null,"abstract":"<div><h3>Background & aim</h3><div>Dental hypersensitivity (DH) is a common issue characterized by discomfort and pain when cold, hot, or acidic substances are consumed. DH treatment aims to control or eliminate causative factors and utilize desensitizing agents to occlude open dentinal tubules; however, there is no standardized definitive treatment yet. The increasing use of lasers, especially diode lasers, along with innovative materials such as Biodentin has contributed to advancing treatments. This study aimed to evaluate and compare the efficacy of a diode laser and Biodentin in the treatment of dentinal tubule occlusion.</div></div><div><h3>Materials & methods</h3><div>In this experimental study, 48 extracted human teeth were randomly divided into four groups: a control group (no treatment), a Biodentine-treated group, a diode laser 660 nm-treated group, and a combination group treated with both laser and Biodentine. After treatment, the samples were examined by scanning electron microscopy (SEM), and the percentage of tubule occlusion was categorized into four levels.</div></div><div><h3>Results</h3><div>A total of 83.3 % of the samples in the combination group (laser and Biodentine) achieved level 3 occlusion (more than 75 % occlusion), whereas 91.7 % of those in the Biodentine-only group were also in level 3. The percentage of patients in the laser-only group was 33.3 % in Level 2 and 66.7 % in Level 1 occlusion.</div></div><div><h3>Conclusion</h3><div>In conclusion, there was no significant difference in the occlusion measurement between the combination treatment group and the Biodentine-only group, with both being primarily rated as Level 3 occlusions. Therefore, the use of a diode laser at 660 nm in conjunction with Biodentine has effects similar to those of Biodentine alone.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 235-239"},"PeriodicalIF":0.0,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oral squamous cell carcinoma (OSCC) has high mortality. Over the decades, there has been not substantial improvement in overall survival which is mostly attributed to lack of effective anticancer agent. Tomatidine, a steroidal alkaloid derived and sourced from unripe green tomatoes, has shown immense anticancer potential. However, its activity in OSCC remains largely uncharacterized. This research aims to evaluate the cytotoxic and pro-apoptotic effects of tomatidine in OSCC.
Method
Target gene for Tomatidine on OSCC treatment was analyzed in the TCGA-HNSC dataset. Two gene were prioritized for network pharmacology to establish their relevance, followed by cheminformatics, drug–target screening, molecular docking, and ADME-T profiling to identify lead compounds. Gene expression and overall survival for target proteins (PI3K/AKT signaling) were examined using GEPIA an cBioportal databases. Functional validation for tomatidine was performed using KB cells via cell viability assay to assess the anticancer effect.
Results
Tomatidine treatment reduced the viability of KB cells in a dosage-dependent manner. The docking simulations showed good binding affinities of tomatidine to PI3K (−8.4 kcal/mol), AKT (−8.8), and PTEN (−10.1), suggesting that tomatidine has a potential ability to disrupt PI3K/Akt signaling and apoptosis.
Conclusion
Tomatidine has potent anticancer effects against OSCC cells inhibiting the PI3K/Akt signaling pathway and promoting apoptosis. These findings highlight the tomatidine utility as a natural anticancer compound.
{"title":"Tomatidine suppresses PI3K/Akt signaling to induce apoptosis in oral squamous cell carcinoma: An in vitro and molecular docking study","authors":"Sadhana Padmanabhan , Palati Sinduja , Monal Yuwanati , Selvaraj Jayaraman , Senthilmurugan Mullainathan","doi":"10.1016/j.jobcr.2025.12.007","DOIUrl":"10.1016/j.jobcr.2025.12.007","url":null,"abstract":"<div><h3>Introduction</h3><div>Oral squamous cell carcinoma (OSCC) has high mortality. Over the decades, there has been not substantial improvement in overall survival which is mostly attributed to lack of effective anticancer agent. Tomatidine, a steroidal alkaloid derived and sourced from unripe green tomatoes, has shown immense anticancer potential. However, its activity in OSCC remains largely uncharacterized. This research aims to evaluate the cytotoxic and pro-apoptotic effects of tomatidine in OSCC.</div></div><div><h3>Method</h3><div>Target gene for Tomatidine on OSCC treatment was analyzed in the TCGA-HNSC dataset. Two gene were prioritized for network pharmacology to establish their relevance, followed by cheminformatics, drug–target screening, molecular docking, and ADME-T profiling to identify lead compounds. Gene expression and overall survival for target proteins (PI3K/AKT signaling) were examined using GEPIA an cBioportal databases. Functional validation for tomatidine was performed using KB cells via cell viability assay to assess the anticancer effect.</div></div><div><h3>Results</h3><div>Tomatidine treatment reduced the viability of KB cells in a dosage-dependent manner. The docking simulations showed good binding affinities of tomatidine to PI3K (−8.4 kcal/mol), AKT (−8.8), and PTEN (−10.1), suggesting that tomatidine has a potential ability to disrupt PI3K/Akt signaling and apoptosis.</div></div><div><h3>Conclusion</h3><div>Tomatidine has potent anticancer effects against OSCC cells inhibiting the PI3K/Akt signaling pathway and promoting apoptosis. These findings highlight the tomatidine utility as a natural anticancer compound.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 226-234"},"PeriodicalIF":0.0,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Human papillomavirus (HPV) infection has emerged as a significant etiological factor in tongue cancer, particularly among individuals without conventional risk factors such as tobacco or alcohol use. Understanding the molecular mechanisms underlying HPV-induced tongue carcinogenesis is critical for advancing targeted interventions.
Objective
To systematically review existing literature on genetic and epigenetic alterations in HPV-associated tongue cancer, with emphasis on viral oncoprotein interactions, disrupted cellular signalling pathways, and immune evasion mechanisms.
Data sources
Electronic databases searched included PubMed, Scopus, Web of Science, Dentistry and Oral Sciences Source, and AMED.
Methods
A systematic search using relevant MeSH terms was conducted to identify peer-reviewed studies involving human participants. Duplicates were removed, and studies were screened using the Rayyan software based on predefined inclusion and exclusion criteria. Quality assessment was performed using the Mixed Methods Appraisal Tool (MMAT).
Results
The search yielded 3,140 articles, with 27 studies meeting the inclusion criteria after full-text screening and manual search. Quality assessment indicated that 96.3 % of studies (n = 26) had low risk of bias, while one study (3.7 %) had high risk. Key findings showed that HPV E6 and E7 oncoproteins interfere with tumor suppressor pathways (e.g., p53 and Rb), and activate PI3K/Akt, Wnt/β-catenin, and NF-κB signaling. Epigenetic alterations such as promoter hypermethylation, histone modification, and microRNA dysregulation were also implicated. HPV-positive tumours demonstrated immune evasion features.
Conclusion
Despite progress in understanding HPV-related tongue carcinogenesis, further research is needed to explore tissue tropism and identify novel therapeutic targets.
Prospero id
CRD42024593129.
人乳头瘤病毒（HPV）感染已成为舌癌的一个重要病因，特别是在没有吸烟或饮酒等传统危险因素的人群中。了解hpv诱发舌癌的分子机制对于推进有针对性的干预至关重要。目的系统回顾hpv相关舌癌遗传和表观遗传改变的现有文献，重点关注病毒癌蛋白相互作用、细胞信号通路中断和免疫逃避机制。检索的电子数据库包括PubMed、Scopus、Web of Science、Dentistry and Oral Sciences Source和AMED。方法使用相关MeSH术语进行系统检索，以识别涉及人类参与者的同行评议研究。删除重复，使用Rayyan软件根据预定义的纳入和排除标准筛选研究。采用混合方法评价工具（MMAT）进行质量评价。结果共检索到3140篇文献，经全文筛选和人工检索，符合纳入标准的文献有27篇。质量评价显示96.3%的研究（n = 26）具有低偏倚风险，1项研究（3.7%）具有高风险。关键发现显示，HPV E6和E7癌蛋白干扰肿瘤抑制通路（如p53和Rb），激活PI3K/Akt、Wnt/β-catenin和NF-κB信号。表观遗传改变，如启动子超甲基化、组蛋白修饰和microRNA失调也有牵连。hpv阳性肿瘤表现出免疫逃避特征。结论尽管对hpv相关舌癌发生的认识有所进展，但仍需进一步研究其组织趋向性和寻找新的治疗靶点。普洛斯彼罗idCRD42024593129。
{"title":"Molecular mechanisms of human papillomavirus-induced tongue carcinogenesis: A systematic review","authors":"Chamathsara Hewa Kodikarage , Menaka Batuwanthudawa , Kalpani Senevirathna , Wasala Mudiyanselage Kalpani Madhushika Ratnayake , Sivasuntharam Induijaa , Yovanthi Anurangi Jayasinghe , Kehinde Kazeem Kanmodi , Bogahawatte Samarakoon Mudiyanselage Samadarani Siriwardena , Ruwan Duminda Jayasinghe","doi":"10.1016/j.jobcr.2025.11.011","DOIUrl":"10.1016/j.jobcr.2025.11.011","url":null,"abstract":"<div><h3>Background</h3><div>Human papillomavirus (HPV) infection has emerged as a significant etiological factor in tongue cancer, particularly among individuals without conventional risk factors such as tobacco or alcohol use. Understanding the molecular mechanisms underlying HPV-induced tongue carcinogenesis is critical for advancing targeted interventions.</div></div><div><h3>Objective</h3><div>To systematically review existing literature on genetic and epigenetic alterations in HPV-associated tongue cancer, with emphasis on viral oncoprotein interactions, disrupted cellular signalling pathways, and immune evasion mechanisms.</div></div><div><h3>Data sources</h3><div>Electronic databases searched included PubMed, Scopus, Web of Science, Dentistry and Oral Sciences Source, and AMED.</div></div><div><h3>Methods</h3><div>A systematic search using relevant MeSH terms was conducted to identify peer-reviewed studies involving human participants. Duplicates were removed, and studies were screened using the Rayyan software based on predefined inclusion and exclusion criteria. Quality assessment was performed using the Mixed Methods Appraisal Tool (MMAT).</div></div><div><h3>Results</h3><div>The search yielded 3,140 articles, with 27 studies meeting the inclusion criteria after full-text screening and manual search. Quality assessment indicated that 96.3 % of studies (n = 26) had low risk of bias, while one study (3.7 %) had high risk. Key findings showed that HPV E6 and E7 oncoproteins interfere with tumor suppressor pathways (e.g., p53 and Rb), and activate PI3K/Akt, Wnt/β-catenin, and NF-κB signaling. Epigenetic alterations such as promoter hypermethylation, histone modification, and microRNA dysregulation were also implicated. HPV-positive tumours demonstrated immune evasion features.</div></div><div><h3>Conclusion</h3><div>Despite progress in understanding HPV-related tongue carcinogenesis, further research is needed to explore tissue tropism and identify novel therapeutic targets.</div></div><div><h3>Prospero id</h3><div>CRD42024593129.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 198-221"},"PeriodicalIF":0.0,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-10DOI: 10.1016/j.jobcr.2025.11.019
Deepavalli Arumuganainar , Pradeep Kumar Yadalam
Background
Chronic inflammation in periodontitis disrupts the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through persistent cytokine activity. IL-1β, TNF-α, and IL-6 are major mediators that inhibit bone-forming pathways. However, the complexity of cytokine–gene interactions remains poorly characterized. This study presents a synthetic transcriptomic modeling framework to predict and interpret inflammatory suppression of stem-cell osteogenesis.
Methods
Time-resolved synthetic gene expression profiles were generated to simulate osteogenic induction under homeostatic, inflammatory, and resolution phases. A curated gene regulatory network (GRN) was incorporated to map cytokine–osteogenesis interactions. Graph autoencoders (GAEs) captured latent topological structure from the expression matrix, while deep neural classifier differentiated inflammatory from control states. The GSE283726 periodontitis transcriptome dataset and iPSC-derived mesenchymal stem cells (iMSCs) were used for validation.
Results
Simulations showed that IL-1β and TNF-α strongly activated NF-κB signaling, suppressing osteogenic genes such as RUNX2 and ALPL. IL-6 exhibited context-dependent regulatory behavior. GAEs clearly separated inflammatory and regenerative modules, identifying IL-6 as a key intermediary. The classifier achieved an AUROC of 0.99 and > 95 % accuracy. Validation with real datasets confirmed overlap in differentially expressed genes and enriched pathways, including Wnt inhibition (DKK1) and inflammatory GO terms.
Conclusion
Biologically informed synthetic transcriptomics combined with graph autoencoding effectively models cytokine-mediated inhibition of PDLSCs. The framework identifies regulatory nodes supported by real data and offers potential for in silico drug testing. Future work will expand cytokine networks, incorporate diverse cell types, and explore transfer learning for regenerative periodontal applications.
{"title":"Predictive modeling of stem cell suppression by inflammatory cytokine networks: A synthetic transcriptomic approach for periodontal tissue engineering","authors":"Deepavalli Arumuganainar , Pradeep Kumar Yadalam","doi":"10.1016/j.jobcr.2025.11.019","DOIUrl":"10.1016/j.jobcr.2025.11.019","url":null,"abstract":"<div><h3>Background</h3><div>Chronic inflammation in periodontitis disrupts the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through persistent cytokine activity. IL-1β, TNF-α, and IL-6 are major mediators that inhibit bone-forming pathways. However, the complexity of cytokine–gene interactions remains poorly characterized. This study presents a synthetic transcriptomic modeling framework to predict and interpret inflammatory suppression of stem-cell osteogenesis.</div></div><div><h3>Methods</h3><div>Time-resolved synthetic gene expression profiles were generated to simulate osteogenic induction under homeostatic, inflammatory, and resolution phases. A curated gene regulatory network (GRN) was incorporated to map cytokine–osteogenesis interactions. Graph autoencoders (GAEs) captured latent topological structure from the expression matrix, while deep neural classifier differentiated inflammatory from control states. The GSE283726 periodontitis transcriptome dataset and iPSC-derived mesenchymal stem cells (iMSCs) were used for validation.</div></div><div><h3>Results</h3><div>Simulations showed that IL-1β and TNF-α strongly activated NF-κB signaling, suppressing osteogenic genes such as RUNX2 and ALPL. IL-6 exhibited context-dependent regulatory behavior. GAEs clearly separated inflammatory and regenerative modules, identifying IL-6 as a key intermediary. The classifier achieved an AUROC of 0.99 and > 95 % accuracy. Validation with real datasets confirmed overlap in differentially expressed genes and enriched pathways, including Wnt inhibition (DKK1) and inflammatory GO terms.</div></div><div><h3>Conclusion</h3><div>Biologically informed synthetic transcriptomics combined with graph autoencoding effectively models cytokine-mediated inhibition of PDLSCs. The framework identifies regulatory nodes supported by real data and offers potential for in silico drug testing. Future work will expand cytokine networks, incorporate diverse cell types, and explore transfer learning for regenerative periodontal applications.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 180-188"},"PeriodicalIF":0.0,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Non-metric dental traits play a pivotal role in anthropological and forensic investigations. Shovel-shaped incisors, in particular, serve as valuable indicators of population affinity and individual identification. This study aimed to assess the frequency and grading of the non-metric shoveling and double shoveling traits in the Mysuru population using the Arizona State University Dental Anthropology System (ASUDAS) scoring criteria.
Methodology
A cross-sectional descriptive study was conducted in Mysuru, among 382 individuals (190 males and 192 females) aged 18–40 years. Traits were assessed clinically using ASUDAS plaques. Data were recorded and analyzed using descriptive statistics and chi-square tests to determine prevalence and statistical significance across gender, arch, quadrant and tooth type.
Results
Shoveling was highly prevalent in the population (95.5 %), especially in upper central incisors (92.2 %), followed by upper lateral incisors (69.3 %). In contrast, lower anterior teeth exhibited minimal expression (<3 %). Double shoveling was rare, observed in only 1.0 % of individuals. No statistically significant difference in trait prevalence was found between genders or sides (p > 0.05). However, shoveling was significantly more common in the upper arch than the lower (p = 0.000). Grading analysis revealed Grade 1 shoveling as the most common form, particularly in upper central and lateral incisors. Double shoveling was mostly absent, with only trace levels (Grade 1) being noted.
Conclusion
The study highlights the prevalence of shoveling, especially in the maxillary anterior teeth among the Mysuru population. The results support the forensic and anthropological value of these traits in personal identification and population studies.
{"title":"Prevalence and distribution of shoveling and double shoveling non-metric traits in Mysuru population: A cross sectional study","authors":"G.R. Aiswarya , H.S. Sreeshyla , Swathi Kumareswar","doi":"10.1016/j.jobcr.2025.12.006","DOIUrl":"10.1016/j.jobcr.2025.12.006","url":null,"abstract":"<div><h3>Background</h3><div>Non-metric dental traits play a pivotal role in anthropological and forensic investigations. Shovel-shaped incisors, in particular, serve as valuable indicators of population affinity and individual identification. This study aimed to assess the frequency and grading of the non-metric shoveling and double shoveling traits in the Mysuru population using the Arizona State University Dental Anthropology System (ASUDAS) scoring criteria.</div></div><div><h3>Methodology</h3><div>A cross-sectional descriptive study was conducted in Mysuru, among 382 individuals (190 males and 192 females) aged 18–40 years. Traits were assessed clinically using ASUDAS plaques. Data were recorded and analyzed using descriptive statistics and chi-square tests to determine prevalence and statistical significance across gender, arch, quadrant and tooth type<strong>.</strong></div></div><div><h3>Results</h3><div>Shoveling was highly prevalent in the population (95.5 %), especially in upper central incisors (92.2 %), followed by upper lateral incisors (69.3 %). In contrast, lower anterior teeth exhibited minimal expression (<3 %). Double shoveling was rare, observed in only 1.0 % of individuals. No statistically significant difference in trait prevalence was found between genders or sides (p > 0.05). However, shoveling was significantly more common in the upper arch than the lower (p = 0.000). Grading analysis revealed Grade 1 shoveling as the most common form, particularly in upper central and lateral incisors. Double shoveling was mostly absent, with only trace levels (Grade 1) being noted.</div></div><div><h3>Conclusion</h3><div>The study highlights the prevalence of shoveling, especially in the maxillary anterior teeth among the Mysuru population. The results support the forensic and anthropological value of these traits in personal identification and population studies.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 189-197"},"PeriodicalIF":0.0,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号