Journal of oral biology and craniofacial research最新文献_第3页

Assessment of cytotoxicity of clear aligners coated with zinc oxide nanoparticles

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-11 DOI: 10.1016/j.jobcr.2025.01.010

Indu Ravi, Vignesh Kailasam

Background

Coating with nanoparticles can have clinical benefits like anti-bacterial activity and therefore an improvement in oral hygiene. However, the toxicity of these coatings is not known. The aim was to assess and compare the viability of human gingival fibroblasts (HGFs) of clear aligners coated with zinc oxide nanoparticles of different sizes.

Methods

Zinc oxide nanoparticles of 30 nm, 60 nm & 90 nm were sputter coated on five aligner samples and were compared with 5 uncoated samples. The thickness of the coating was standardized at 100 nm. The cytotoxicity on HGFs was assessed at day 0, 7 and 14 using assay ([3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]).

Results

The coated aligners exhibited “slight-cytotoxicity” on day 7 and day 14 compared to the uncoated aligners which were categorized as “no cytotoxicity”. Though the cell viability decreased in both the coated and uncoated aligners from day 0 to day 7 to day 14, this decrease was statistically significant for the coated aligners only. (p < 0.05) The greater cytotoxicity observed for the coated aligners was statistically significant only on day 14 (p < 0.001). Within the coated aligners, the 90 nm coating showed the least cytotoxicity, when compared with 60 nm and 30 nm at the time points measured, but this difference was significant only between the 90 nm and 60 nm group on day 14.

Conclusion

The mean decrease in cell viability for the uncoated aligner within the “no cytotoxicity range” while it was within the “slight cytotoxicity range” for the coated aligner. An increase in particle size demonstrated lesser cytotoxicity. ,.Zinc oxide coated aligners should be used with caution after day 7 even though there is only slight toxicity.

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引用次数: 0

Effect of age on the treatment duration and proposed “impacted incisor severity score” of impacted maxillary incisor: A retrospective study

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-11 DOI: 10.1016/j.jobcr.2025.01.024

Umer Mukhtar, Manoj kumar Jaiswal, Rigzen Tamchos, Ashima Goyal, Satinder Pal Singh, Aditi Kapur

Background

Absence of anterior maxillary teeth has a significantly impact on esthetics, speech, mastication, and psychosocial well-being in young patients.

Materials and methods

A retrospective study was conducted from January 2012 to January 2022, the data include children (8–15 years) with maxillary impacted incisors. After initial screening of the digital records, 75 cases with 80 impacted maxillary central incisors were recruited; 9 cases were excluded due to incomplete records.

Results

75 patients were found to have impacted incisors with a prevalence of 0.21 %. Patients were then divided into group 1 (<11years) and group 2 (11–15years) with a mean age of 9.51 ± 1.2 and 13.31 ± 1.74, respectively. Patients who reported at 11–15 years of age were significantly more as compared to <11years (p = 0.014). Among the various etiological factors, physical obstruction or inadequate space was found to be most common. Among dilacerated teeth, 75 % had a history of trauma, and only 35 % of normal impacted central incisors had a history of trauma (p = 0.00139). The mean duration of treatment time was found to be 16.77 ± 8.83months and 21.75 ± 6.08months for impacted incisors with normal morphology and with dilaceration, respectively and the difference was statistically significant (p = 0.042).

Conclusions

Considering the age of the patient, it does not show significant effect on the duration of treatment, but a change in root morphology like dilaceration, significantly increases the duration of treatment. In addition, the proposed objective scoring system “Impacted incisor severity score” could help the clinicians to assess the difficulty and duration of treatment of impacted incisors.

{"title":"Effect of age on the treatment duration and proposed “impacted incisor severity score” of impacted maxillary incisor: A retrospective study","authors":"Umer Mukhtar, Manoj kumar Jaiswal, Rigzen Tamchos, Ashima Goyal, Satinder Pal Singh, Aditi Kapur","doi":"10.1016/j.jobcr.2025.01.024","DOIUrl":"10.1016/j.jobcr.2025.01.024","url":null,"abstract":"<div><h3>Background</h3><div>Absence of anterior maxillary teeth has a significantly impact on esthetics, speech, mastication, and psychosocial well-being in young patients.</div></div><div><h3>Materials and methods</h3><div>A retrospective study was conducted from January 2012 to January 2022, the data include children (8–15 years) with maxillary impacted incisors. After initial screening of the digital records, 75 cases with 80 impacted maxillary central incisors were recruited; 9 cases were excluded due to incomplete records.</div></div><div><h3>Results</h3><div>75 patients were found to have impacted incisors with a prevalence of 0.21 %. Patients were then divided into group 1 (<11years) and group 2 (11–15years) with a mean age of 9.51 ± 1.2 and 13.31 ± 1.74, respectively. Patients who reported at 11–15 years of age were significantly more as compared to <11years (p = 0.014). Among the various etiological factors, physical obstruction or inadequate space was found to be most common. Among dilacerated teeth, 75 % had a history of trauma, and only 35 % of normal impacted central incisors had a history of trauma (p = 0.00139). The mean duration of treatment time was found to be 16.77 ± 8.83months and 21.75 ± 6.08months for impacted incisors with normal morphology and with dilaceration, respectively and the difference was statistically significant (p = 0.042).</div></div><div><h3>Conclusions</h3><div>Considering the age of the patient, it does not show significant effect on the duration of treatment, but a change in root morphology like dilaceration, significantly increases the duration of treatment. In addition, the proposed objective scoring system “Impacted incisor severity score” could help the clinicians to assess the difficulty and duration of treatment of impacted incisors.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 297-304"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical efficacy of Methylene Blue mediated antimicrobial Photodynamic Therapy (PDT) using 680 nm Diode Laser in patients undergoing Open Flap Debridement: An Original Research

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-11 DOI: 10.1016/j.jobcr.2025.01.022

Soundarya Singh, Manisha Rout, Mayur Kaushik, Shilpa Sharma, Aprajita Srivastava

Background

Inflammatory periodontal disease caused by dental plaque is characterised by the clinical signs of inflammation and loss of periodontal tissue support. The mechanical removal of this biofilm and adjunctive use of antibacterial disinfectants and antibiotics have been the conventional methods of periodontal therapy. The possibility of the development of resistance to antibiotics by the target organism has led to the development of a new antimicrobial concept with fewer complications. Photodynamic therapy (PDT) involves the use of low-power lasers with appropriate wavelengths to kill microorganisms treated with a photosensitiser drug. PDT could be a useful adjunct to mechanical as well as antibiotics in eliminating periodontal-pathogenic bacteria.

Methods

The patients selected were randomly divided into two groups. In the test group, Open Flap Debridement (OFD) with Methylene Blue-mediated Antimicrobial Photodynamic Therapy (PDT) using 680 nm Diode Laser application was done, and in the control group, OFD alone.

Results

The results of this study showed that diode laser irradiation (680 nm) combined with methylene blue dye reduced pocket probing depth and gingival bleeding by up to 95.85 % in the group treated with PDT with 5 min of irradiation.

Conclusions

The adjunctive use of photodynamic therapy with Diode 680 nm Laser and Methylene Blue Photosensitiser can be beneficial in reducing pocket probing depth, plaque index, and gingival bleeding index. Antimicrobial photodynamic therapy may hold promise as a substitute for currently available chemotherapy in the treatment of periodontal and peri-implant disease and in endodontic therapy.

{"title":"Clinical efficacy of Methylene Blue mediated antimicrobial Photodynamic Therapy (PDT) using 680 nm Diode Laser in patients undergoing Open Flap Debridement: An Original Research","authors":"Soundarya Singh, Manisha Rout, Mayur Kaushik, Shilpa Sharma, Aprajita Srivastava","doi":"10.1016/j.jobcr.2025.01.022","DOIUrl":"10.1016/j.jobcr.2025.01.022","url":null,"abstract":"<div><h3>Background</h3><div>Inflammatory periodontal disease caused by dental plaque is characterised by the clinical signs of inflammation and loss of periodontal tissue support. The mechanical removal of this biofilm and adjunctive use of antibacterial disinfectants and antibiotics have been the conventional methods of periodontal therapy. The possibility of the development of resistance to antibiotics by the target organism has led to the development of a new antimicrobial concept with fewer complications. Photodynamic therapy (PDT) involves the use of low-power lasers with appropriate wavelengths to kill microorganisms treated with a photosensitiser drug. PDT could be a useful adjunct to mechanical as well as antibiotics in eliminating periodontal-pathogenic bacteria.</div></div><div><h3>Methods</h3><div>The patients selected were randomly divided into two groups. In the test group, Open Flap Debridement (OFD) with Methylene Blue-mediated Antimicrobial Photodynamic Therapy (PDT) using 680 nm Diode Laser application was done, and in the control group, OFD alone.</div></div><div><h3>Results</h3><div>The results of this study showed that diode laser irradiation (680 nm) combined with methylene blue dye reduced pocket probing depth and gingival bleeding by up to 95.85 % in the group treated with PDT with 5 min of irradiation.</div></div><div><h3>Conclusions</h3><div>The adjunctive use of photodynamic therapy with Diode 680 nm Laser and Methylene Blue Photosensitiser can be beneficial in reducing pocket probing depth, plaque index, and gingival bleeding index. Antimicrobial photodynamic therapy may hold promise as a substitute for currently available chemotherapy in the treatment of periodontal and peri-implant disease and in endodontic therapy.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 266-270"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyaluronic acid versus amniotic membrane in wound healing and bone regeneration in extraction sockets - A randomized controlled trial

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-11 DOI: 10.1016/j.jobcr.2025.01.027

Abinav Sundar T. , Pratiksha Shetty , Padmaraj Hegde , Shreya S.

Background

Surgical removal of the impacted third molar is a routinely employed procedure in oral surgery, often associated with various complications which affect wound healing. Hyaluronic acid (HA) and freeze-dried (lyophilized) amniotic membrane (AM) have demonstrated the potential to promote wound healing and bone regeneration. These could aid in the healing of the extraction socket post-disimpaction.

Objectives

To assess the extent of wound healing and bone regeneration in extraction sockets of surgically removed mandibular third molars following intra-socket application of 0.2 % HA gel and 2.5 × 2.5 cm of AM.

Material and methods

45 patients were clinically and radiographically evaluated based on the inclusion and exclusion criteria and were randomized by lottery method into three groups – Group 1, control, Group 2, AM, and Group 3, HA. The pain scores were evaluated using the visual analog scale. The extent of facial swelling, trismus and bone regeneration were assessed at three different time intervals.

Results

A significant difference (p<0.05) in the pain score was observed between the control group and the study groups. The extent of facial swelling and trismus observed was of significance within the groups (p<0.05). Group 2 exhibited significantly improved levels of trabecular bone formation at the third post-operative month (p<0.05).

Conclusion

HA and AM could be potentially useful in improving the post-operative sequelae following surgical removal of mandibular third molars in terms of pain, wound healing, and overall bone regeneration.

{"title":"Hyaluronic acid versus amniotic membrane in wound healing and bone regeneration in extraction sockets - A randomized controlled trial","authors":"Abinav Sundar T. , Pratiksha Shetty , Padmaraj Hegde , Shreya S.","doi":"10.1016/j.jobcr.2025.01.027","DOIUrl":"10.1016/j.jobcr.2025.01.027","url":null,"abstract":"<div><h3>Background</h3><div>Surgical removal of the impacted third molar is a routinely employed procedure in oral surgery, often associated with various complications which affect wound healing. Hyaluronic acid (HA) and freeze-dried (lyophilized) amniotic membrane (AM) have demonstrated the potential to promote wound healing and bone regeneration. These could aid in the healing of the extraction socket post-disimpaction.</div></div><div><h3>Objectives</h3><div>To assess the extent of wound healing and bone regeneration in extraction sockets of surgically removed mandibular third molars following intra-socket application of 0.2 % HA gel and 2.5 × 2.5 cm of AM.</div></div><div><h3>Material and methods</h3><div>45 patients were clinically and radiographically evaluated based on the inclusion and exclusion criteria and were randomized by lottery method into three groups – Group 1, control, Group 2, AM, and Group 3, HA. The pain scores were evaluated using the visual analog scale. The extent of facial swelling, trismus and bone regeneration were assessed at three different time intervals.</div></div><div><h3>Results</h3><div>A significant difference (<em>p</em><0.05) in the pain score was observed between the control group and the study groups. The extent of facial swelling and trismus observed was of significance within the groups (<em>p</em><0.05). Group 2 exhibited significantly improved levels of trabecular bone formation at the third post-operative month (<em>p</em><0.05).</div></div><div><h3>Conclusion</h3><div>HA and AM could be potentially useful in improving the post-operative sequelae following surgical removal of mandibular third molars in terms of pain, wound healing, and overall bone regeneration.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 305-309"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative efficacy of LLLT and LED therapy on osteoblast proliferation and osteoclast suppression: An in vitro co-culture study

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-11 DOI: 10.1016/j.jobcr.2025.01.018

Dr Divyabharathi Selvam, Dr Venkat Rengasamy

Background

Dental implants are a reliable solution for tooth loss, but challenges remain regarding osseointegration and complications. Photobiomodulation therapy (PBMT), including Low-Level Laser Therapy (LLLT) and Light Emitting Diode (LED) therapy, has shown potential in enhancing bone healing. However, comparative studies on their effects on osteoblasts and osteoclasts are limited.

Aim

To compare the effects of LLLT and LED therapy on osteoblast proliferation and osteoclast suppression in an in vitro co-culture model.

Materials and methods

MG-63 osteoblast-like cells and RAW 264.7 osteoclasts were co-cultured on titanium discs. Discs were treated with LLLT (940 nm, 50 mW) or LED (660 nm, 89 mW) for different durations. Osteoblast proliferation was measured by MTT assay, and osteoclast activity was evaluated using TRAP staining and resorption pit analysis.

Results

LED therapy significantly enhanced osteoblast proliferation and suppressed osteoclast activity more effectively than LLLT. Optimal results were observed with a 6-min LED exposure.

Conclusion

LED therapy shows greater potential than LLLT for promoting osteoblast proliferation and suppressing osteoclast activity, indicating its clinical value for improving bone regeneration and implant integration.

{"title":"Comparative efficacy of LLLT and LED therapy on osteoblast proliferation and osteoclast suppression: An in vitro co-culture study","authors":"Dr Divyabharathi Selvam, Dr Venkat Rengasamy","doi":"10.1016/j.jobcr.2025.01.018","DOIUrl":"10.1016/j.jobcr.2025.01.018","url":null,"abstract":"<div><h3>Background</h3><div>Dental implants are a reliable solution for tooth loss, but challenges remain regarding osseointegration and complications. Photobiomodulation therapy (PBMT), including Low-Level Laser Therapy (LLLT) and Light Emitting Diode (LED) therapy, has shown potential in enhancing bone healing. However, comparative studies on their effects on osteoblasts and osteoclasts are limited.</div></div><div><h3>Aim</h3><div>To compare the effects of LLLT and LED therapy on osteoblast proliferation and osteoclast suppression in an in vitro co-culture model.</div></div><div><h3>Materials and methods</h3><div>MG-63 osteoblast-like cells and RAW 264.7 osteoclasts were co-cultured on titanium discs. Discs were treated with LLLT (940 nm, 50 mW) or LED (660 nm, 89 mW) for different durations. Osteoblast proliferation was measured by MTT assay, and osteoclast activity was evaluated using TRAP staining and resorption pit analysis.</div></div><div><h3>Results</h3><div>LED therapy significantly enhanced osteoblast proliferation and suppressed osteoclast activity more effectively than LLLT. Optimal results were observed with a 6-min LED exposure.</div></div><div><h3>Conclusion</h3><div>LED therapy shows greater potential than LLLT for promoting osteoblast proliferation and suppressing osteoclast activity, indicating its clinical value for improving bone regeneration and implant integration.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 325-330"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNA

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-10 DOI: 10.1016/j.jobcr.2025.01.030

Lina M. Escobar , Zita Bendahan , Deisy Abril , David Díaz-Baez , Adolfo Paternina , Farith González-Martinez , Ma Clara González-Carrera

The quantity and quality of DNA are crucial factors in determining polymorphisms in samples from individuals with and without pathologies such as cleft lip and palate (CL/P). Saliva is increasingly being evaluated as a collection method. This study aimed to compare saliva DNA concentrations from individuals of different ages with and without CL/P.

Saliva samples were collected, DNA was extracted and the quantification was performed by spectrophotometry. Kruskal-Wallis test was used to compare frequencies between groups, and Spearman's rho for correlations.

We obtained saliva DNA from 314 individuals: 107 with CL/P (mean age: 12.7 ± 6.5 years), 103 parents of children with CL/P (mean age: 41.1 ± 9.3 years), 52 individuals without CL/P under 18 years old (controls), and 52 individuals without CL/P over 18 years old (controls). The sample comprised 32.2 % females and 67.8 % males.

Statistically significant differences in DNA concentrations were found between individuals under and over 18 years old (12.38 and 21.3 ng/μl, respectively, p = 0.0001), but no differences were observed between males and females. Individuals with CL/P had lower DNA concentrations (12.45 ng/μl) compared to their parents and controls (21.95 and 21.15 ng/μl, respectively). Our results showed a direct correlation between age and DNA concentration in individuals with CL/P under 18 years old (R = 0.328).

{"title":"Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNA","authors":"Lina M. Escobar , Zita Bendahan , Deisy Abril , David Díaz-Baez , Adolfo Paternina , Farith González-Martinez , Ma Clara González-Carrera","doi":"10.1016/j.jobcr.2025.01.030","DOIUrl":"10.1016/j.jobcr.2025.01.030","url":null,"abstract":"<div><div>The quantity and quality of DNA are crucial factors in determining polymorphisms in samples from individuals with and without pathologies such as cleft lip and palate (CL/P). Saliva is increasingly being evaluated as a collection method. This study aimed to compare saliva DNA concentrations from individuals of different ages with and without CL/P.</div><div>Saliva samples were collected, DNA was extracted and the quantification was performed by spectrophotometry. Kruskal-Wallis test was used to compare frequencies between groups, and Spearman's rho for correlations.</div><div>We obtained saliva DNA from 314 individuals: 107 with CL/P (mean age: 12.7 ± 6.5 years), 103 parents of children with CL/P (mean age: 41.1 ± 9.3 years), 52 individuals without CL/P under 18 years old (controls), and 52 individuals without CL/P over 18 years old (controls). The sample comprised 32.2 % females and 67.8 % males.</div><div>Statistically significant differences in DNA concentrations were found between individuals under and over 18 years old (12.38 and 21.3 ng/μl, respectively, p = 0.0001), but no differences were observed between males and females. Individuals with CL/P had lower DNA concentrations (12.45 ng/μl) compared to their parents and controls (21.95 and 21.15 ng/μl, respectively). Our results showed a direct correlation between age and DNA concentration in individuals with CL/P under 18 years old (R = 0.328).</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 246-249"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-10 DOI: 10.1016/j.jobcr.2025.01.026

Lukas Mendes de Abreu , Cintia Rodrigues da Silva , Ana Laura Ferreira Bortoleto , Giovana Barros Nunes , Matheus Martins Gracia , Rafael Akira Tzanno Murayama , Daniel Galera Bernabé , Gisele Zoccal Mingoti

Objectives

This study aimed to investigate oxidative stress markers in the oral mucosal cells of individuals diagnosed with major depressive disorder (MDD).

Methods

A case-control design was used, including twenty patients diagnosed with MDD, based on the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) criteria, and twenty healthy controls. Oral exfoliated cells were collected from all participants. Intracellular levels of reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), caspase-3 and -7 activity, and reduced glutathione (GSH) were measured in Arbitrary Fluorescence Units (AFU).

Results

The MDD group demonstrated significantly elevated intracellular ROS levels (p = 0.0012) and caspase-3 and -7 activity (p = 0.0171) in comparison to the control group. Additionally, a decrease in ΔΨm expression was observed in the oral cells of MDD patients (p = 0.0265), whereas GSH expression levels did not differ significantly between the two groups (p = 0.8908).

Conclusions

The findings indicate heightened oxidative stress in the oral exfoliated cells of individuals with MDD. This study supports the potential use of oral cells as a non-invasive biomarker source for assessing oxidative stress in depressive disorders.

{"title":"Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder","authors":"Lukas Mendes de Abreu , Cintia Rodrigues da Silva , Ana Laura Ferreira Bortoleto , Giovana Barros Nunes , Matheus Martins Gracia , Rafael Akira Tzanno Murayama , Daniel Galera Bernabé , Gisele Zoccal Mingoti","doi":"10.1016/j.jobcr.2025.01.026","DOIUrl":"10.1016/j.jobcr.2025.01.026","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to investigate oxidative stress markers in the oral mucosal cells of individuals diagnosed with major depressive disorder (MDD).</div></div><div><h3>Methods</h3><div>A case-control design was used, including twenty patients diagnosed with MDD, based on the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) criteria, and twenty healthy controls. Oral exfoliated cells were collected from all participants. Intracellular levels of reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), caspase-3 and -7 activity, and reduced glutathione (GSH) were measured in Arbitrary Fluorescence Units (AFU).</div></div><div><h3>Results</h3><div>The MDD group demonstrated significantly elevated intracellular ROS levels (p = 0.0012) and caspase-3 and -7 activity (p = 0.0171) in comparison to the control group. Additionally, a decrease in ΔΨm expression was observed in the oral cells of MDD patients (p = 0.0265), whereas GSH expression levels did not differ significantly between the two groups (p = 0.8908).</div></div><div><h3>Conclusions</h3><div>The findings indicate heightened oxidative stress in the oral exfoliated cells of individuals with MDD. This study supports the potential use of oral cells as a non-invasive biomarker source for assessing oxidative stress in depressive disorders.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 256-261"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the cost-effectiveness of prosthetic rehabilitation for partially edentulous teeth in India: A comparative study of Clasp-Retained RPDs, Thermoplastic Resin RPDs, and Cast Partial Dentures

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-10 DOI: 10.1016/j.jobcr.2025.01.014

Subhash V , Aswini Kumar K , Vineetha Karuveettil

Background

With rising life expectancy, partial edentulism has increased, particularly in developing nations where economic constraints, limited awareness, and inadequate dental care exacerbate the burden on aging populations. Patients with fewer than 20 teeth often experience a reduction in Oral Health-Related Quality of Life (OHRQoL), emphasizing the need for effective tooth replacement. Removable partial dentures (RPDs) are versatile, cost-effective solutions for partial tooth loss, including metal clasp-retained RPDs (MC-RPDs), thermoplastic resin RPDs (TR-RPDs), and cast partial dentures (CPDs).

Methods

This study evaluates the oral health-related quality of life, patient satisfaction, and cost-effectiveness of these three RPD types. A cross-sectional study was conducted with 42 participants, divided into three groups: MC-RPD, TR-RPD, and CPD, each with 14 patients. The Oral Health Impact Profile (OHIP-14) and Satisfaction Questionnaire (SAT) were administered at baseline and three months post-denture delivery. The study adhered to Consolidated Health Economic Evaluation Reporting Standards.

Results

Results showed significant differences in OHIP scores between the groups, with CPD offering the highest quality of life, followed by TR-RPD, and MC-RPD. Post-treatment, the mean OHIP scores for MC-RPD, TR-RPD, and CPD were 30.57 ± 4.09, 20.71 ± 4.03, and 22.36 ± 2.92, respectively, with CPD showing the greatest improvement. Patient satisfaction was highest for CPD, followed by TR-RPD, and least for MC-RPD. However, satisfaction differences between the groups were not statistically significant after three months.

Conclusion

This study highlights the superior performance of CPDs in enhancing OHRQoL and patient satisfaction, though their higher cost may limit accessibility. These findings provide valuable insights for dental practitioners and patients in selecting the most suitable and cost-effective RPD treatment options.

{"title":"Evaluating the cost-effectiveness of prosthetic rehabilitation for partially edentulous teeth in India: A comparative study of Clasp-Retained RPDs, Thermoplastic Resin RPDs, and Cast Partial Dentures","authors":"Subhash V , Aswini Kumar K , Vineetha Karuveettil","doi":"10.1016/j.jobcr.2025.01.014","DOIUrl":"10.1016/j.jobcr.2025.01.014","url":null,"abstract":"<div><h3>Background</h3><div>With rising life expectancy, partial edentulism has increased, particularly in developing nations where economic constraints, limited awareness, and inadequate dental care exacerbate the burden on aging populations. Patients with fewer than 20 teeth often experience a reduction in Oral Health-Related Quality of Life (OHRQoL), emphasizing the need for effective tooth replacement. Removable partial dentures (RPDs) are versatile, cost-effective solutions for partial tooth loss, including metal clasp-retained RPDs (MC-RPDs), thermoplastic resin RPDs (TR-RPDs), and cast partial dentures (CPDs).</div></div><div><h3>Methods</h3><div>This study evaluates the oral health-related quality of life, patient satisfaction, and cost-effectiveness of these three RPD types. A cross-sectional study was conducted with 42 participants, divided into three groups: MC-RPD, TR-RPD, and CPD, each with 14 patients. The Oral Health Impact Profile (OHIP-14) and Satisfaction Questionnaire (SAT) were administered at baseline and three months post-denture delivery. The study adhered to Consolidated Health Economic Evaluation Reporting Standards.</div></div><div><h3>Results</h3><div>Results showed significant differences in OHIP scores between the groups, with CPD offering the highest quality of life, followed by TR-RPD, and MC-RPD. Post-treatment, the mean OHIP scores for MC-RPD, TR-RPD, and CPD were 30.57 ± 4.09, 20.71 ± 4.03, and 22.36 ± 2.92, respectively, with CPD showing the greatest improvement. Patient satisfaction was highest for CPD, followed by TR-RPD, and least for MC-RPD. However, satisfaction differences between the groups were not statistically significant after three months.</div></div><div><h3>Conclusion</h3><div>This study highlights the superior performance of CPDs in enhancing OHRQoL and patient satisfaction, though their higher cost may limit accessibility. These findings provide valuable insights for dental practitioners and patients in selecting the most suitable and cost-effective RPD treatment options.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 240-245"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finite element stress analysis to evaluate and compare the effect of splinting in periodontally compromised teeth having 55 % bone loss with different materials

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-10 DOI: 10.1016/j.jobcr.2025.01.011

Shivangi Joshi, Prakash Talreja, Prajakta Rao, Nilesh Joshi, Vinayak Thorat, Ruhi Mohta

Background

Splinting is commonly employed to stabilize periodontally compromised teeth, but the choice of splint material significantly influences stress distribution within the periodontal ligament (PDL) and surrounding bone. This study aimed to evaluate and compare the stress distribution of four different splint materials—composite, fiber-reinforced composite (FRC), polyetheretherketone (PEEK), and metal—on mandibular anterior teeth with 55 % bone loss using finite element analysis (FEA).

Methodology

Finite element models of mandibular anterior teeth with 55 % bone loss were developed using SOLIDWORKS 2020. Simulations were conducted for non-splinted teeth and for teeth splinted with composite, FRC, PEEK, and metal. Stress analysis was performed in ANSYS software under vertical (100N at 0°) and oblique (100N at 45°) loading conditions. Von Mises stress values in the PDL and cortical bone were recorded and statistically analyzed using MedCalc software to compare the performance of different splint materials.

Results

Non-splinted teeth exhibited the highest stress levels, particularly under oblique loading conditions. Among the splinting materials, FRC showed the most effective reduction in stress across all teeth, especially under vertical loads. Composite and metal wire splints provided moderate stress reduction, with performance varying depending on the load angle. PEEK splints demonstrated good stress reduction under vertical loads but showed increased stress levels under oblique forces. These results underscore the influence of splint material and load direction on stress distribution in periodontally compromised teeth.

Conclusion

The study highlights the critical role of splint material in reducing stress on the PDL of periodontally compromised teeth. FRC splints emerged as the most effective material for minimizing stress under both vertical and oblique loading conditions. Composite and metal wire splints offered moderate efficacy, while PEEK splints were less effective under oblique loads. These findings provide valuable insights for clinicians in selecting optimal splint materials for periodontal stabilization.

{"title":"Finite element stress analysis to evaluate and compare the effect of splinting in periodontally compromised teeth having 55 % bone loss with different materials","authors":"Shivangi Joshi, Prakash Talreja, Prajakta Rao, Nilesh Joshi, Vinayak Thorat, Ruhi Mohta","doi":"10.1016/j.jobcr.2025.01.011","DOIUrl":"10.1016/j.jobcr.2025.01.011","url":null,"abstract":"<div><h3>Background</h3><div>Splinting is commonly employed to stabilize periodontally compromised teeth, but the choice of splint material significantly influences stress distribution within the periodontal ligament (PDL) and surrounding bone. This study aimed to evaluate and compare the stress distribution of four different splint materials—composite, fiber-reinforced composite (FRC), polyetheretherketone (PEEK), and metal—on mandibular anterior teeth with 55 % bone loss using finite element analysis (FEA).</div></div><div><h3>Methodology</h3><div>Finite element models of mandibular anterior teeth with 55 % bone loss were developed using SOLIDWORKS 2020. Simulations were conducted for non-splinted teeth and for teeth splinted with composite, FRC, PEEK, and metal. Stress analysis was performed in ANSYS software under vertical (100N at 0°) and oblique (100N at 45°) loading conditions. Von Mises stress values in the PDL and cortical bone were recorded and statistically analyzed using MedCalc software to compare the performance of different splint materials.</div></div><div><h3>Results</h3><div>Non-splinted teeth exhibited the highest stress levels, particularly under oblique loading conditions. Among the splinting materials, FRC showed the most effective reduction in stress across all teeth, especially under vertical loads. Composite and metal wire splints provided moderate stress reduction, with performance varying depending on the load angle. PEEK splints demonstrated good stress reduction under vertical loads but showed increased stress levels under oblique forces. These results underscore the influence of splint material and load direction on stress distribution in periodontally compromised teeth.</div></div><div><h3>Conclusion</h3><div>The study highlights the critical role of splint material in reducing stress on the PDL of periodontally compromised teeth. FRC splints emerged as the most effective material for minimizing stress under both vertical and oblique loading conditions. Composite and metal wire splints offered moderate efficacy, while PEEK splints were less effective under oblique loads. These findings provide valuable insights for clinicians in selecting optimal splint materials for periodontal stabilization.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 250-255"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of upper airway airflow dynamics in young adults with isolated Robin sequence: An exploratory investigation

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-02-10 DOI: 10.1016/j.jobcr.2025.01.009

Maria Noel Marzano-Rodrigues , Sergio Henrique Kiemle Trindade , Ivy Kiemle Trindade-Suedam

Impaired upper airway dimension in adults with Isolated Robin Sequence (IRS) can alter airflow dynamics, increasing the risk of pharyngeal collapse and the onset of obstructive sleep apnea. This study aimed to characterize the upper airways of six young adults (20.83 ± 6.40 years) with IRS, using computational fluid dynamics. Upper airways of six patients were reconstructed using 3D segmentation, generating unstructured hybrid meshes with ≥4 million tetrahedral elements. Flow simulations at 15 l/min were solved using the realizable k-ε model and the finite volume method. Morphophysiological variables assessed were: total airway volumes, minimal cross-sectional areas, average pressure, velocity magnitude, wall shear stress, turbulent kinetic energy (k) production, and resistance. Airway volume corresponded to 29.32 ± 4.65 cm³ and minimal cross-sectional area was 1.00 ± 0.55 cm². Pressure drop, airway resistance to airflow, velocity of the airflow and turbulent kinetic energy corresponded to 31.341 ± 15.837 Pa, 0.125 ± 0.063 (Pa s/ml), 1.882 ± 0.514 (m/s) and 0.152 ± 0.056 (m2/s2). The total airway volume exhibited a strong negative correlation with airway resistance (−0.899) and the inlet-to-outlet pressure drop (−0.899). The minimal cross-sectional area of the pharynx at the retroglossal level showed a strong negative correlation (−0.912) with the area-weighted average velocity magnitude of the airflow and with k production (−0.924). In conclusion, airway volume reduction and retroglossal obstruction in young adults with IRS are associated with altered fluid flow characteristics, including increased velocity magnitude, pressure drop, resistance, and turbulent kinetic energy production. These changes may increase the effort to breathe and predispose patients to sleep-disordered breathing.

{"title":"Characterization of upper airway airflow dynamics in young adults with isolated Robin sequence: An exploratory investigation","authors":"Maria Noel Marzano-Rodrigues , Sergio Henrique Kiemle Trindade , Ivy Kiemle Trindade-Suedam","doi":"10.1016/j.jobcr.2025.01.009","DOIUrl":"10.1016/j.jobcr.2025.01.009","url":null,"abstract":"<div><div>Impaired upper airway dimension in adults with Isolated Robin Sequence (IRS) can alter airflow dynamics, increasing the risk of pharyngeal collapse and the onset of obstructive sleep apnea. This study aimed to characterize the upper airways of six young adults (20.83 ± 6.40 years) with IRS, using computational fluid dynamics. Upper airways of six patients were reconstructed using 3D segmentation, generating unstructured hybrid meshes with ≥4 million tetrahedral elements. Flow simulations at 15 l/min were solved using the realizable k-ε model and the finite volume method. Morphophysiological variables assessed were: total airway volumes, minimal cross-sectional areas, average pressure, velocity magnitude, wall shear stress, turbulent kinetic energy (k) production, and resistance. Airway volume corresponded to 29.32 ± 4.65 cm³ and minimal cross-sectional area was 1.00 ± 0.55 cm<sup>2</sup>. Pressure drop, airway resistance to airflow, velocity of the airflow and turbulent kinetic energy corresponded to 31.341 ± 15.837 Pa, 0.125 ± 0.063 (Pa s/ml), 1.882 ± 0.514 (m/s) and 0.152 ± 0.056 (m2/s2). The total airway volume exhibited a strong negative correlation with airway resistance (−0.899) and the inlet-to-outlet pressure drop (−0.899). The minimal cross-sectional area of the pharynx at the retroglossal level showed a strong negative correlation (−0.912) with the area-weighted average velocity magnitude of the airflow and with k production (−0.924). In conclusion, airway volume reduction and retroglossal obstruction in young adults with IRS are associated with altered fluid flow characteristics, including increased velocity magnitude, pressure drop, resistance, and turbulent kinetic energy production. These changes may increase the effort to breathe and predispose patients to sleep-disordered breathing.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 2","pages":"Pages 234-239"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0