Journal of Nutrition最新文献

Background: Till date, no studies have investigated the effects of cardiovascular health on the cardioprotective benefits of fish oil supplementation in people with type 2 diabetes (T2D). The utility of fish oil in reducing the risk of cardiovascular disease (CVD) mortality in people with diabetes remains unclear and inconsistent.

Objective: To investigate the potential modifying effect of cardiovascular health (CVH) level, as assessed using the Life's Essential 8 (LE8) score, on the association between regular fish oil supplement use and CVD mortality in middle-aged and older people with T2D.

Methods: Participants with T2D in the UK Biobank were included. CVH level was categorized by the mean LE8 score (55 points). Multivariable-adjusted Cox models were used to evaluate the longitudinal association between regular use of fish oil supplements and CVD mortality. We performed stratified analysis across different CVH levels, and tested for potential interaction between fish oil supplement use and CVH level.

Results: The analysis included 19,003 participants (mean age 59.9±6.9 years, 36.1% women), of whom 39.6% were regular users of fish oil supplements. During a median follow-up of 13.7 years, regular use of fish oil supplements was significantly associated with a lower risk of CVD mortality among participants with better CVH (i.e., LE8 score ≥55 points; hazard ratio [HR]=0.65, 95% confidence interval [CI] 0.51-0.84, P = 0.001), but not among those with poorer CVH (i.e., LE8 score <55 points; HR=1.02, 95% CI 0.83-1.25, P=0.867). The interaction between the use of fish oil supplements and CVH level on CVD mortality was significant (P=0.018).

Conclusions: Middle-aged and older T2D individuals with a relatively low baseline CVH level may not obtain cardiovascular benefits from fish oil supplements. Our findings highlight the importance of promoting multimodal lifestyle interventions to improve survival outcomes of people with T2D.

Background: The pro-fertility diet is a dietary pattern composed of nutrients and foods most consistently associated with in vitro fertilization (IVF) endpoints in women.

Objective: We examined the potential biological mechanisms underlying the association between adherence to a pro-fertility diet and IVF endpoints using high-resolution metabolomics.

Methods: Among 120 women undergoing an autologous oocyte IVF cycle (2007-2015) in Northeast United States, we collected a serum sample during controlled ovarian stimulation and a follicular fluid sample on the day of oocyte retrieval. Women completed a food frequency questionnaire upon enrollment into the study to examine adherence to the pro-fertility diet pattern. Liquid chromatography with high-resolution mass spectrometry was used for untargeted metabolomics analysis of biospecimens. We identified metabolic features (and enriched biological pathways) associated with the pro-fertility diet and two IVF endpoints, live birth and clinical pregnancy, via a meet-in-the-middle approach.

Results: In the follicular fluid metabolome, vitamin D3 metabolism was associated with adherence to the pro-fertility diet pattern and live birth. Additionally, vitamin D3 metabolism, vitamin B6 metabolism, and bile acid biosynthesis, were associated with both adherence to the pro-fertility diet pattern and clinical pregnancy. In the serum metabolome, only tryptophan metabolism was associated with adherence to the pro-fertility diet pattern and live birth. We confirmed the chemical identity of one metabolite with Level-1 evidence, 4-pyridoxate, which was higher in the serum and follicular fluid among women with stronger adherence to the pro-fertility diet pattern and among women with a live birth.

Conclusions: The beneficial association between adherence to the pro-fertility diet and IVF outcomes may be mediated through vitamin D3 metabolism, vitamin B6 metabolism, and bile acid biosynthesis in the follicular fluid and tryptophan metabolism in the serum. These results provide new insight in the important biological pathways underlying a dietary pattern providing optimal fertility benefits to women.

Background: Retinopathy of prematurity (ROP) is a leading cause of blindness in infants, affecting 32% of hospitalized preterm babies. Oxidative stress, a primary pathogenic factor in ROP, triggers abnormal neovascularization of retinal vessels. Lutein, an antioxidant and the main component of macular pigment, is found in low levels in preterm infants and may help ameliorate ROP. However, its low bioavailability limits its application as a nutritional intervention.

Objective: The aim of the study was to assess the effect of a lutein emulsion stabilized by a food-grade biopolymer on lutein bioavailability in neonatal rats with ROP and examine the effects of both unemulsified lutein and lutein emulsion on the disease.

Methods: Neonatal rats were subcutaneously administered KRN 633 (10 mg/kg BW) on postnatal days 7 and 8 (P7 and P8) to induce ROP. Neonatal rats that did not receive the treatment served as the control. From P9 to P21, both ROP and non-ROP rats were divided into three groups and given daily doses of olive oil, unemulsified lutein (2 mg/kg BW lutein), or lutein emulsion (2 mg/kg BW lutein). On P22, serum and tissues were collected. Lutein concentrations were measured using UPLC, and retinal morphology was assessed using immunohistochemistry.

Results: Rats treated with lutein emulsion had significantly higher serum and tissue lutein concentrations than those receiving unemulsified lutein. Morphological assessments showed that ROP rats had more tortuous arteries, increased capillary density, enlarged vessels, reduced astrocyte density, and decreased neuronal cells. Both unemulsified lutein and lutein emulsion alleviated these abnormalities, with lutein emulsion showing superior efficacy in restoring neuronal cell levels to normal in the peripheral retina.

Conclusions: Lutein, in both unemulsified and emulsified forms, effectively inhibited ROP progression in neonatal rats. The biopolymer-based lutein emulsion showed promise as a delivery system to enhance lutein bioavailability and mitigate ROP in preterm infants.

Probiotics, prebiotics, and postbiotics can significantly influence B-cell-related diseases through their immunomodulatory effects. They enhance the immune system's function, particularly affecting B cells, which originate in the bone marrow and are crucial for antibody production and immune memory. These substances have therapeutic potential in managing allergies, autoimmune diseases, and inflammatory conditions by regulating the gut microbiota, strengthening epithelial barriers, and directly interacting with various components of the innate and adaptive immune systems. The review highlights the critical need for further research into the precise mechanisms through which probiotics, prebiotics, and postbiotics modulate B cells. Gaining this understanding could facilitate the development of more effective treatments for B-cell-related diseases by harnessing the immunomodulatory properties of these dietary components.

Background: Globally, 148 million children under 5 are stunted, with risk factors varying by context. Our 'Impact of Growth Charts and Nutritional Supplements on Child Growth in Zambia' (ZamCharts) trial observed persistently high rates of stunting in all treatment groups after 18-months of intervention with monthly distributions of small-quantity lipid-based nutrient supplements (SQ-LNS) and/or installation of a wall-mounted growth chart in children's homes.

Objectives: We sought to identify determinants of stunting and height-for-age z-score in children 27-36 months who participated in the ZamCharts endline survey (n=1911).

Methods: Multi-level, log-binomial models were used to estimate univariable and multivariable prevalence ratios for predictors of stunting. Multi-level models were also used to predict height-for-age z-score (HAZ) with and without baseline HAZ (assessed at ages 2-11 months). We also conducted a path analysis using covariance analysis of linear structural equations to assess underlying and modifiable risk factors for impaired linear growth.

Results: Significant predictors of stunting in the multivariable model included: low asset ownership; being male; using biomass as cooking fuel; lower maternal height; a mother with ≤ primary education; lower baseline HAZ. and not being randomized to SQ-LNS. Significant predictors of a lower mean HAZ in the full multivariable models included all the same risk factors that predicted stunting, but also living in an urban area, having more than one child <5 years in the household and diarrhea in the previous 2 weeks. The multivariable model explained 48% of variability in endline HAZ; the strongest predictor was baseline HAZ, which explained 29% of endline HAZ variability in the univariable model.

Conclusions: Preventing stunting in Zambia will require investments in early life (pre- and post-natal) determinants of growth trajectory as well as improving complementary feeding practices and addressing risk factors for infectious diseases; SQ-LNS can also improve linear growth and reduce stunting.

Background: Epidemiological evidence linking blood pressure (BP) and body weight-lowering effects with fruit and vegetable consumption mostly relies on self-reported dietary assessment prone to misreport and under- or overestimation of relationships. We characterized objective 24-hr urinary metabolites and a derived metabolite score associated with fruit and vegetable intake and assessed their associations with BP and body mass index (BMI), with validation across cohorts.

Methods: We used untargeted proton nuclear magnetic resonance spectroscopy (¹H NMR) of two timed repeated 24-hr urine collections from free-living participants from the US (n=2,032) and the UK (n=449) of the cross-sectional International Study of Macro-/Micronutrients and Blood Pressure (INTERMAP). We evaluated correlations between fruit and vegetable intake assessed by 24-hr dietary recalls with 7,100 ¹H NMR features, adjusted for confounders and multiple testing. We related identified metabolites and a metabolite score with BP and BMI using extensively adjusted multiple linear regression models.

Results: We characterized eleven ¹H NMR-derived 24-hr urinary metabolites related to fruit and vegetable intake, reproducible across multiple 24-hr urine collections of both cohorts. Proline betaine, citrate, N-methylproline, scyllo-inositol, 2-hydroxy-2-(4-methyl cyclohex-3-en-1-yl) propoxyglucuronide, and proline were associated with fruit intake, specifically with Rutaceae intake, while S-methyl-L-cysteine sulfoxide and S-methyl-L-cysteine sulfoxide metabolite were associated with Brassicacea intake. The metabolite score, explaining 39.8% of fruit and vegetable intake, was inversely associated with systolic BP (-1.65 mmHg; 95% confidence interval (CI): -2.68,-0.62, P<0.002) and BMI (-1.21 kg/m²; 95% CI: -1.62,-0.78, P<0.0001). These associations were to a large extent explained by urinary citrate excretion.

Conclusion: We identified ¹H NMR-derived urinary metabolites associated with fruit and vegetable consumption, consistent and reproducible between urine collections and across populations. A higher fruit and vegetable-related metabolite score showed associations with lower systolic BP and BMI, mainly mediated by citrate, but would need confirmation in further studies.

Study registration: This study was registered at the clinicaltrials.gov registration (https://clinicaltrials.gov/study/NCT00005271?term=NCT00005271&rank=1) as NCT00005271.

Phytochemicals are non-nutritive substances found in plant foods that originally contribute significantly to the flavor and color of the foods. These substances are usually classified as polyphenols, terpenes, sulfur-containing compounds, nitrogen containing compounds and others. A large amount studies from last decades demonstrated that these substances have played an immeasurable role in physiological regulation, health care and disease prevention through the exerting actions of antioxidation, anti-inflammation, anti-ageing, anti-virus, anti-cancer, anti-thrombosis, regulating lipid profiles, cardiovascular protection, neuroprotection, immunity regulation and improving metabolic functions. This paper is intended to review the chemistry and biochemistry of phytochemicals as related to classification, chemical structure, their occurrence and biosynthesis in plants, and the biological activities and implications in human health and various diseases. The discussions are focused on important and most recent advances in the above aspects. Furthermore, some future research directions of phytochemicals are set forth in the aspects of the dose-response, the mechanism and targets, the interactions with gut microbiota, the impact on human health and different stages of chronic diseases.

Background: Food insecurity (FI) continues to be a significant public health concern and is associated with myriad physical and mental health consequences. Increased understanding of conditions around its occurrence throughout the life course are needed. However, research has been limited due to inadequate measurement tools and study length.

Objective: This study examined the intra- and intergenerational dynamics of FI over time by assessing the transmission of FI from childhood to adulthood, and from mother to offspring using population-specific FI measures, and the influence of sociodemographic factors.

Methods: Women in early midlife (n=624) and their children (n=331) participated in a prospective cohort study between 1987-2019 in Richmond, California. Three validated FI measures were assessed, representing 1) past childhood FI and 2) current adult household FI, reported by the women, and 3) current child FI, reported by the women's children. Associations between measures were examined using adjusted modified Poisson regression models. Mediation by current adult household FI between past childhood FI and their offspring's current child FI was assessed. Moderation by sociodemographic factors, including poverty level, parental education, marital status, and race was also assessed.

Results: Among women, 32.4% reported FI in childhood and 34.5% reported current adult household FI. Among their offspring, 53.2% reported current child FI. Past childhood FI increased the likelihood of current adult FI (RR: 2.18, 95%CI: 1.64, 2.90) and current adult FI increased the likelihood of current child FI (RR:1.49, 95%CI: 1.08, 2.07). Current adult FI partially mediated past childhood FI and their offspring's current child FI (Natural indirect effect: 1.42, 95%CI: 1.03, 2.24). There was no evidence of moderation by sociodemographic factors.

Conclusions: FI measures reported by adults and children capture differing experiences, highlighting the need to use FI measures that are appropriate for their target population. FI may be perpetuated intra- and intergenerationally.

Ambient concentrations of the air pollutant, ozone, are rising with increasing global temperatures. Ozone is known to increase incidence and exacerbation of chronic lung diseases, which will increase as ambient ozone levels rise. Studies have identified diet as a variable able to modulate the pulmonary health effects associated with ozone exposure. Eicosapentaenoic Acid (EPA) is an n-3 polyunsaturated fatty acid (PUFA) consumed through diet that lowers inflammation through conversion to oxylipins including Hydroxy Eicosapentaenoic Acids (HEPEs). However, the role of dietary EPA in ozone-induced pulmonary inflammation is unknown. Therefore, we hypothesize increasing dietary EPA will decrease ozone-induced pulmonary inflammation and injury through the production of HEPEs. To test this, male C57BL/6J mice were fed a purified control diet or EPA-supplemented diet for 4 weeks and then exposed to filtered air (FA) or 1 part per million ozone for 3 hours. 24 or 48 hours post exposure, bronchoalveolar lavage (BAL) fluid was collected to assess airspace inflammation/injury and lung tissue was collected for targeted Liquid Chromatography-Mass Spectrometry (LC-MS/MS) lipidomics. Following ozone exposure, EPA supplementation did not alter markers of lung injury, but decreased ozone-induced airspace neutrophilia. LC-MS/MS targeted lipidomics reveal dietary EPA supplementation increased pulmonary EPA-derived metabolites including 5-HEPE and 12-HEPE. Additionally, EPA supplementation decreased pulmonary levels of pro-inflammatory Arachidonic Acid (AA)-derived metabolites. To evaluate if dietary EPA reduces ozone-induced pulmonary inflammation through increased pulmonary HEPEs, C57BL/6J mice were administered 5- and 12-HEPEs systemically prior to filtered air or ozone exposure. Pretreatment with 5- and 12-HEPEs reduced ozone-driven increases in airspace macrophages. Together, these data indicate that an EPA-supplemented diet protects against ozone-induced airspace inflammation which is, in part, due to increasing pulmonary concentrations of 5- and 12-HEPEs. These findings suggest that dietary EPA and/or increasing EPA-derived metabolites in the lung can reduce ozone-driven incidences and exacerbations of chronic pulmonary diseases.