Journal of Nutrition最新文献

Background: Red and processed meat intake is associated with type 2 diabetes (T2D) incidence, whereas plant-based protein has inverse associations. There is limited knowledge of white meat intake and its effects on T2D risk. Intake of saturated fatty acids (SFAs) may modify these associations.

Objectives: Our objective was to assess the effects of controlled intake of red meat, white meat, and nonmeat protein sources and concentration of SFAs, keeping weight stable, on glucose homeostasis measures, including disposition index.

Methods: Secondary analysis was performed of a subset of participants (n = 46) who underwent frequently sampled oral glucose tolerance testing from a randomized, controlled crossover diet intervention trial (n = 113 in main trial) including adults without cardiovascular disease or diabetes. Diet interventions assessed effect of concentration of SFAs (14% compared with 7%) in 2 arms, and protein source (12% red meat, white meat, and nonmeat) in a crossover design with 4-wk feeding periods and 2-7-wk washout periods. Insulin sensitivity, insulin secretion rate, and disposition index were assessed using linear mixed models.

Results: There were no baseline differences in participant demographic and clinical variables between the 2 SFA arms. We found no significant differences in insulin sensitivity or static and dynamic insulin secretion rate and disposition index by protein source or concentration of SFA (P > 0.05).

Conclusions: In a short-term diet intervention trial in healthy individuals, neither protein source nor concentration of SFAs significantly affects disposition index as a proxy for diabetes risk. This trial was registered at clinicaltrials.gov as NCT01427855.

Background: Independently, diet and exercise can benefit bone health, but they are seldom studied in combination.

Objectives: We investigated how dietary supplementation with tart cherry (TC) alone and in combination with exercise affects bone, and whether this response differs with age.

Methods: Two cohorts of female C57BL/6 mice (6 wk old; n = 12 mice/group and 60 wk old; n = 14 mice/group) were assigned to diets (control vs. with TC diet) and exercise (with vs. without treadmill running) for 8 wks. Bone microarchitecture, biomechanics, and gene expression of regulators of bone cell differentiation and activity were assessed. Data were analyzed using 2-way analysis of variance followed by post hoc testing. Diet effects on serum untargeted metabolomics were analyzed using unpaired t-tests. For all analyses, α = 0.05.

Results: Diet and exercise differentially affected young and aging mice. Both TC (P < 0.0001) and exercise (P < 0.05) independently increased femoral trabecular bone in young mice. Although exercise failed to increase cortical parameters, it improved bone strength. In the aging cohort, TC treatment improved cortical thickness (P < 0.05) and biomechanical properties of the femur. The effects of TC and exercise were not additive in either cohort. Within the bone of young mice, TC increased (P < 0.01) the expression of bone morphogenetic protein 2 (Bmp2), a regulator of osteoblast differentiation and decreased (P < 0.01) matrix metalloproteinases (Mmp8 and Mmp9), involved in bone matrix degradation. In the aging mice, TC increased (P < 0.05) regulators of osteoblast differentiation (Osterix and Bmp2), and TC and exercise independently increased collagen type 1A, indicative of osteoblast activity. Metabolomics revealed increases in xenometabolites from TC and the gut microbiota (e.g., hippuric acid, methyltyrosine, and methylgalactoside) in both cohorts, which positively correlated with bone parameters.

Conclusions: We conclude there was no added benefit of combining TC with the running protocol used in this study. TC had a pronounced osteogenic effect in both age groups and metabolomic profiling revealed new potential mechanisms by which its bone effects are mediated.