Journal of oleo science最新文献

Biological activities observed in living systems occur as the output of which nanometer-, submicrometer-, and micrometer-sized structures and tissues non-linearly and dynamically behave through chemical reaction networks, including the generation of various molecules and their assembly and disassembly. To understand the essence of the dynamic behavior in living systems, simpler artificial objects that exhibit cell-like non-linear phenomena have been recently constructed. However, most objects exhibiting cell-like dynamics have been found through trial-and-error experiments, and there are no strategies for designing them as molecular systems. This review describes how cell-like dynamics of oil droplets in surfactant solution, such as self-propelled motion, chemotaxis, division, and deformation, are induced by combining molecular properties of system components toward self-propelled microrobots.

The current study was designed to evaluate the antibacterial, antibiofilm, and biofilm inhibitory potential of six medicinal plants, including Trachyspermum ammi, Trigonella foenum-graecum, Nigella sativa, Thymus vulgaris, Terminalia arjuna, and Ipomoea carneaid against catheter-associated bacteria (CAB). Eighteen CAB were identified up to species level using 16S rRNA gene sequencing, viz., Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa. T. ammi essential oil and T. foenum-graecum methanolic extract combination exhibited the highest antibacterial activity (ZOI; 32.0) against S. aureus. N. sativa essential oil (EO) showed highest ZOI (31.0; p ≤ 0.05) against Proteus mirabilis at 100 µgmL ^-1 . Among 18 CAB isolated, 13 showed mature biofilm formation on 5 ^th day. All plant extracts demonstrated more than 80% antibiofilm and biofilm inhibition activity. A concentrationdependent increase was observed with plant extracts against CAB during antibacterial, antibiofilm, and biofilm inhibition activities. The study suggests that EO and methanolic extract (ME) of tested plants possess promising antibiofilm and biofilm inhibitory potential against CABs. To our knowledge, this is the first study to report antibacterial, antibiofilm, and biofilm inhibitory potential of T. ammi and N. sativa seed EO, as well as T. foenum-graecum, N. sativa, T. vulgaris, T. arjuna, and I. carnea ME against CAB from medical setting.

Based on the observation that urea, water, and ethyl esters (EE) can form gypsum-like mixtures, this study explored the feasibility of employing water as a solvent for urea in the urea complexation method to enrich n-3 polyunsaturated fatty acids with docosahexaenoic acid (DHA)-containing ethyl esters (DHA- EE) from Crypthecodinium cohnii as the material. Under the conditions of a urea/DHA-EE ratio of 3, a water/DHA-EE ratio of 0.75, a mixing temperature of 65℃, and a cooling temperature of 20℃, a concentrate containing over 90% DHA was achieved. This demonstrated that using water as a solvent for urea, instead of polar organic solvents, is feasible and efficient for enriching DHA in urea complexation process.

Cleaning and sterilization are critical Prerequisite Programs in sanitation management based on HACCP. Most food factories clean and sanitize equipment daily after production using detergents containing benzalkonium chloride (BAC). However, in factories that produce oil and fat-rich foods, it has been discovered that microbes can persist on production equipment. Insufficient cleaning protocols may result in secondary contamination of the final products. Unfortunately, there are limited cleaning agents available that are effective in sterilizing microbes in the presence of oil. Moreover, there is a lack of research on the bactericidal mechanisms and bacterial dynamics in oily environments. In this study, we aimed to reduce bacterial contamination on equipment in such factories by hypothesizing that oil diminishes BAC's bactericidal activity. We conducted lab-scale experiments simulating actual factory conditions to examine the effects of oil on BAC's efficacy. Additionally, we investigated the effect of nonionic surfactants, which are known to enhance BAC's bactericidal activity in oil-free conditions, in the presence of oil. The results showed that BAC's bactericidal activity was significantly reduced in the presence of oil. However, the activity was restored by adding an appropriate amount of secondary alcohol ethoxylate (sec-AE). Microscopic observations revealed that bacteria tended to accumulate at the water/oil interface, suggesting that the oil interface might inhibit BAC from effectively attacking the bacteria. The addition of sec-AE appeared to disperse the bacteria into the water layer, thus restoring BAC's bactericidal activity in the presence of oil. These findings are crucial for improving daily cleaning and sterilization processes in food factories operating in high-oil environments to prevent secondary contamination and enhance food safety.

Omega-3 long-chain polyunsaturated fatty acids (PUFA) such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are widely used as supplements and pharmaceuticals because of their beneficial effects on human health. Triacylglycerols (TAG) and glycerophospholipids (GPL) comprise the primary chemical structures of DHA/EPA in marine sources. Furthermore, DHA/EPA-enriched glycerophospholipids (DHA/EPA-GPL) and lysoglycerophospholipids (DHA/EPA-LysoGPL) consumed through food and supplements are more effective than TAG in promoting health, which may be attributed to a specific underlying mechanism. However, the specific effects of DHA/EPA bound to GPL structure have been still unclear. The aim of this review is to clarify the significance of the binding of DHA/EPA to GPL in promoting the health benefits of DHA/EPA-GPL and DHA/EPA-LysoGPL. Additionally, the potential use of fishery by-products as sources of DHA/EPA-GPL and DHA/EPA-LysoGPL has been discussed.

In this study, the role of roasting on the total phenol, antioxidant capacity, phenolic constituents and fatty acid profile of the grape seeds was investigated. Total phenolic and flavonoid quantities of the grape seeds roasted in microwave (MW) and conventional oven (CO) systems were recorded between 673.57 (control) and 713.57 (MW) to 7121.67 (MW) and 7791.67 mg/100 g (CO), respectively. Antioxidant activities of the grape seeds varied between 6.57 (MW) and 7.24 mmol/kg (control). Catechin and rutin quantities of the grape seeds were recorded to be between 435.30 (CO) and 581.57 (control) to 94.94 (CO) and 110.53 mg/100 g (MW), respectively. While gallic acid amounts of the seed samples are established between 21.06 (control) and 101.79 (MW), quercetin values of the grape seeds were assigned to be between 56.59 (control) and 77.81 mg/100 g (CO). In addition, p-coumaric acid and resveratrol quantities of the grape seeds were recorded between 15.43 (control) and 22.98 (CO) to 12.50 (CO) and 29.57 mg/100 g (MW), respectively. The main fatty acids in oil samples were linoleic, oleic, palmitic and stearic acids in decreasing order. Linoleic and oleic acid values of the oils provided from grape seeds were recorded to be between 72.75 (control) and 73.33% (MW) to 14.79 (CO) and 14.87% (MW), respectively. It was observed that the element results related to the grape seed differed based on the roasting type when compared to the control. The most abundant elements in the grape seed were K, P, Mg, S, Na, Fe, Ca, Zn, and K and P amounts of the grape seeds were reported to be between 6706.93 (MW) and 7089.33 (control) to 2764.27 (CO) and 2927.97 mg/kg (control), respectively. It is thought that it would be beneficial to add grape seeds to foods as an ingredient by taking into account these phytochemical components as a result of the applied heat treatment.

Aquilaria agallocha is an economically valuable plant facing endangerment, sought after globally for its production of agarwood. A. agallocha tree possesses medicinal and aromatic properties in its fruits, branches, leaves, wood, and roots, which are being studied for their effective compounds and their potential bioactive effects on human health. This study aimed to uncover the phytochemical properties and biological activities of A. agallocha roots, which possess medicinal and aromatic characteristics. The phenolic, organic acid, and sugar profiles of A. agallocha roots were determined using HPLC-DAD, while mineral distributions were analyzed via ICP-AES. In vitro antioxidant capacity was assessed using the 2,2-diphenyl- 1-picrylhydrazyl (DPPH) assay. Furthermore, the antibacterial effects of methanol extracts of A. agalocha roots against S. aureus, S. carnosus, B. cereus, L. monocytogenes, E. coli, L. innocua, K. pneumoniae, and E. faecalis were estimated with disc diffusion and MIC methods. The amount of total phenolic and in vitro DPPH radical scavenging capacity of A. agalocha roots were determined as 7.529 mg GAE/g and 18.83 µmol TE/g, respectively. Eight phenolic components were detected in the extracts of A. agalocha roots. The most dominant among these phenolic compounds detected was tannic acid, which was followed by 4-hydrobenzoic acid and oleuropein, respectively. In the aqueous extracts of A. agalocha roots, four organic acids were identified, including citric, tartaric, malic and succinic acid, and succinic acid was the most dominant organic acid. Only fructose sugar was detected in the water extracts of A. agalocha roots. The extracts of A. agalocha roots had a strong antibacterial effect against all gram-positive pathogens except for S. carnosus, but did not have any effect against gram-negative bacteria.

Coelomic fluid of earthworms is a valuable source of novel bioactive compounds with therapeutic applications. To gain insight into the bioactive compounds in the coelomic fluid, this study used Perionyx excavatus, a tropical earthworm distinguished for its remarkable ability for regeneration. This study aimed to identify fluorescent bioactive compounds in the coelomic fluid of P. excavatus and to investigate these compounds structural and functional characteristics for potential use in biomedical applications. Fluorescent bioactive compounds present in the coelomic fluid are identified using Thin Layer Chromatography (TLC), UV-visible spectrophotometry, and Spectrofluorometry techniques. Two unknown groups of fluorophore, named CFA and CFB, were analyzed by studying their emission spectra. In addition, GC-MS and LC-MS analyses provides detailed list of bioactive compound present in the coelomic fluid, in which indole and arachidonic acid shown maximum excitation and thus chosen for further studies. Their functional characterization reveals antibacterial activity, cytotoxicity and in-vitro wound healing assays, respectively. Notably, both of them exhibit significant efficacy against Aeromonas hydrophila, Salmonella typhi and Staphylococcus aureus. However, indole shows poor activity against Pseudomonas aeruginosa, whereas arachidonic acid demonstrates effective activity. These findings imply that these bioactive fluorescent compounds may have significant therapeutic applications.

The present study aimed to explore the potential of macroalgal hydrolysate to serve as an economical substrate for the growth of the oleaginous microbes Aspergillus sp. SY-70, Rhizopus arrhizus SY-71 and Aurantiochytrium sp. YB-05 for lipid and DHA production under laboratory conditions. The macroalgal hydrolysate was used at three concentrations 20, 40 and 80 g/L as a sole carbon source or in combination with 10 g/L of either acetic acid, glycerol, glucose, or sugarcane molasses. Glucose was used as a positive control at four different concentrations: 10, 20, 40, and 80 g/L. Out of the 19 carbon sources tested for the three microbes, 80 g/L macroalgae + 10 g/L molasses was the best source for Aspergillus sp. SY-70 (27.4 g/L DW and 9.73 g/L lipid) and R. arrhizus SY-71 (49.76 g/L DW and 16.88 g/L lipid), whereas 20 g/L macroalgae + 10 g/L glucose afforded the best source for Aurantiochytrium sp. YB-05 (27.93 g/L DW and 11.07 g/L lipid). Among the 19 carbon sources used for the growth of Aurantiochytrium sp. YB-05, we determined the fatty acid profile of the best four carbon sources that gave the highest biomass and lipid percentage. Among the four sources, 20 g/L macroalgal hydrolysate + glucose gave the highest DHA percentage (2.31 g/L), followed by 80 g/L pure glucose (1.68), 80 g/L macroalgal hydrolysate + glycerol (1.64), and 40 g/L macroalgal hydrolysate + molasses (1.52). The three carbon sources can replace pure glucose for the lipid, DPA, and DHA production using Aurantiochytrium sp. YB-05. The results of the current study suggest that we could use macroalgal hydrolysate in combination with molasses or glucose for the production of single-cell oil.