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Bacillus circulans as biosurfactant-producer during crude oil degradation 环状芽孢杆菌在原油降解过程中作为生物表面活性剂的生产者
Pub Date : 2023-01-29 DOI: 10.4314/sa.v21i3.13
E. Michael, I. Idemudia
Petroleum and its byproducts are one group of universal environmental pollutants. Microorganisms have over time played significant roles in the clean-up exercise of unwanted substances in the environment. This research was aimed at studying the degradative potentials of biosurfactantproducing bacterial isolates (Bacillus sp.) from palm oil mill effluent (POME) in crude oil degradation. Standard microbiological and analytical methods were applied to ascertain biosurfactant production and degradation of crude oil by Bacillus sp. isolated from palm oil mill effluent discharged points and logging area of effluent bunk at Nigerian Institute for Oil-Palm Research (NIFOR) in Edo State. The bacteria were isolated and subjected to screening for hydrocarbon degradation and biosurfactant production. Biosurfactants characterization by Fourier Transform Infra-Red (FTIR) technique. total viable heterotrophic bacterial count of POME and Bonny light crude oil ranged from 6.6 x106 – 8.2 x106 cfu/ml and 4.2 x106 – 5.8 x106 cfu/ml respectively. Bacillus sp. that had the highest biodegradative potential and biosurfactant production was identified molecularly as Bacillus circulans. It could be used as bio-stimulants to ameliorate crude oil polluted areas as an efficient and cost- effective technology.
石油及其副产品是一类普遍存在的环境污染物。随着时间的推移,微生物在清理环境中不需要的物质方面发挥了重要作用。本研究旨在研究棕榈油厂废水(POME)中产生生物表面活性剂的分离菌(芽孢杆菌)在原油降解中的降解潜力。采用标准的微生物学和分析方法,确定从埃户州尼日利亚油棕研究所(NIFOR)的棕榈油厂废水排放点和废水层采伐区分离的芽孢杆菌sp.生产和降解原油的生物表面活性剂。对分离得到的细菌进行了烃类降解和生物表面活性剂生产的筛选。傅里叶变换红外(FTIR)技术表征生物表面活性剂。POME和Bonny轻质原油的活异养细菌总数分别为6.6 × 106 ~ 8.2 × 106 cfu/ml和4.2 × 106 ~ 5.8 × 106 cfu/ml。从分子上鉴定出具有最高生物降解潜力和生物表面活性剂产量的芽孢杆菌为环状芽孢杆菌。它可以作为一种高效、经济的生物刺激剂用于改善原油污染地区。
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引用次数: 0
Application of 2-D imaging survey for ascertaining the cause(s) of road failures along Sapele/Agbor road in Delta State, Nigeria 二维成像调查在确定尼日利亚三角洲州Sapele/Agbor公路沿线道路故障原因中的应用
Pub Date : 2023-01-29 DOI: 10.4314/sa.v21i3.17
O. Akpoyibo, O. Anomohanran, C. Ossai
Surveying involving 2-D imaging dipole–dipole geophysical technique was carried out along Sapele-Agbor Highway situated in Southern, Nigeria to determine the primary causes for Highway pavement failure. Twelve profiles spreading over 100 meters were in each cases conducted on road surface sections along the stable (control) and unstable portions of the road. Data were gathered together along the various profile employing ABEM Terra meter SAS 1000. The field data were processed and inverted utilizing Dipro software. This was done by specifying and locating the geoelectric arrangement within the surface, sub-base and sub grade soil to obtain the confirming cause(s) of the road associated with pavement failure. Geoelectric sections identified four geologic layers embracing the topsoil, clayey, laterite and sand. The sections failure is due to disparity of the material used for road model (construction) as some comprises of clay materials. The results show the presence of low resistivity values at several subdivisions of the 12 profiles. Resistivity values varying from 21.60 Ωm – 1026.0 Ωm to a depth of 14.92 m from the surface was observed along Okpara Water side, Aghalokpe and Otumara. In Igun, Eku and Obiaruku resistivity values ranges from 10.5 Ωm – 500.0 Ωm to a depth of 5.5 m from the topsoil, 22 m from sub grade and 9.8 m - 50 m were observed along the four profiles. Urhomehe, Urhonigbe, Abavo and Agbor indicate resistivity ranging from 59.25 Ωm – 1467.50 Ωm for stable and unstable sections of the road with depth up to 15.0 m for some profiles. Low resistivity values, not greater than 199 Ωm observed in some distinctive regions of 12 profiles comprises of expansive, compressible, collapsible and sandy clay materials which have the capacity of absorbing water. These make the roads swell and collapse leading to road failure under stress and strain. Many stable portions of the road are characterized with high resistivity value greater than 199 Ωm, mostly laterite materials. Competent fill soil should replace low resistivity soil to a depth of 5 m – 7m from the surface of the road. Good drainage system is also recommended within the unstable road sections.
在尼日利亚南部的Sapele-Agbor高速公路沿线进行了二维成像偶极子-偶极子地球物理技术测量,以确定高速公路路面损坏的主要原因。在每个案例中,沿着道路的稳定(控制)部分和不稳定部分的路面路段进行了12个分布超过100米的剖面图。采用ABEM Terra SAS 1000测量仪沿不同剖面收集数据。利用Dipro软件对现场资料进行处理和反演。这是通过指定和定位地面、下层和下层土壤中的地电排列来完成的,以获得与路面破坏相关的道路的确认原因。地电剖面确定了四个地质层,包括表土、粘土、红土和沙子。部分路段的破坏是由于用于道路模型(施工)的材料不一致,其中一些包括粘土材料。结果表明,在12条剖面的几个细分处存在低电阻率值。在Okpara水侧、Aghalokpe和Otumara观测到的电阻率值从21.60 Ωm - 1026.0 Ωm到距离地表14.92 m的深度。在Igun、Eku和Obiaruku,沿4条剖面观测到的电阻率值范围为10.5 Ωm ~ 500.0 Ωm至距表土5.5 m,距路基22 m和9.8 m ~ 50 m。Urhomehe、Urhonigbe、Abavo和Agbor显示稳定和不稳定路段的电阻率范围为59.25 Ωm - 1467.50 Ωm,某些剖面的深度可达15.0 m。在12条剖面的一些特殊区域观测到的低电阻率值不大于199 Ωm,包括具有吸水能力的膨胀、可压缩、可折叠和砂质粘土材料。这些使道路膨胀和坍塌,导致道路在压力和应变下失效。许多稳定路段的电阻率值均大于199 Ωm,且多为红土材料。填方土应取代低电阻率土,填方土距路面5 m - 7m。在不稳定的路段内,亦建议设置良好的排水系统。
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引用次数: 1
Effects of viscous dissipation and joule heating of magnetohydrodynamics (MHD) convective nano fluid heat transfer over a flat porous plate 粘性耗散和焦耳加热对平面多孔板上磁流体动力学(MHD)对流纳米流体传热的影响
Pub Date : 2023-01-29 DOI: 10.4314/sa.v21i3.14
A. Musa, F. E. Bazuaye
In the present investigation, we studied the effects of Viscous Dissipation and Joule Heating of magnetohydrodynamic (MHD) convective heat transfer over a flat porous plate. The governing partial differential equations is expressed into a nonlinear partial differential equation, using a suitable similarity transformation. A semi analytical method of Homotopy Parturbation was applied to solve the equation and the obtained numerical solution for the velocity, temperature and other parameters of the nano fluid are discussed and represented graphically. Also, the effect of other parameters on the velocity and temperature profiles are also presented.
在本研究中,我们研究了粘滞耗散和焦耳加热对平面多孔板上磁流体动力对流换热的影响。通过适当的相似变换,将控制偏微分方程表示为非线性偏微分方程。采用同伦半解析法对方程进行求解,讨论了纳米流体的速度、温度等参数的数值解,并用图形表示。此外,还讨论了其他参数对速度和温度分布的影响。
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引用次数: 0
Impacts of functional groups on the intermolecular forces of attraction in animal glue obtained from the femur bones of cow (Bos taurus) 功能基团对牛大腿骨动物胶分子间引力的影响
Pub Date : 2022-09-08 DOI: 10.4314/sa.v21i2.3
T. Chikwe, R. Duru
Functional groups in samples obtained from the femur bone of Cow (Bos taurus) were determined using Shidmazu Fourier transform infrared (FT-IR) – 8300 spectroscopy. The bones were crushed, washed properly with warm water and immersed in lime solution for three days. Samples were then treated with dilute 20% hydrochloric acid (to release the collagen in the bone) dried and crushed to a particle size of 0.02 mm. Results obtained from FT-IR analyses shows the presence of alcohol, alkyne, carboxylic acid, phosphate, 1,3 disubstituted aromatics and haloalkanes at wavelength ranges of 3550 – 3200 cm-1, 2360 – 2014 cm-1, 1420 – 1320 cm-1, 1099 – 1039 cm-1, 970 – 890 cm-1 and 705 – 550 cm-1 respectively. The presence of functional groups with highly electronegative atoms such as two, three and four oxygen atoms in carboxylic acid, anhydrides and phosphates respectively in addition to the presence of halogens in haloalkanes and multiple bonds in alkyne, nitrile and alkenes increases the polarizability of the compounds thereby strengthening the weak intermolecular forces of attraction such as hydrogen bond and Van der Waal forces of the adhesive molecules which ultimately increases the adhesive strength and the force of cohesion of the animal glue produced.
利用Shidmazu傅里叶变换红外(FT-IR) - 8300光谱对牛股骨样品中的官能团进行了测定。骨头被压碎,用温水适当清洗,并在石灰溶液中浸泡三天。然后用稀释20%的盐酸(以释放骨头中的胶原蛋白)处理样品,干燥并压碎至0.02毫米的粒度。FT-IR分析结果表明,在3550 ~ 3200 cm-1、2360 ~ 2014 cm-1、1420 ~ 1320 cm-1、1099 ~ 1039 cm-1、1970 ~ 890 cm-1和705 ~ 550 cm-1波长范围内分别存在醇、炔、羧酸、磷酸、1,3二取代芳烃和卤代烷。除了卤代烷中存在卤素和炔中存在多个键外,羧酸、酸酐和磷酸盐中还存在具有高电负性原子的官能团,如两个氧原子、三个氧原子和四个氧原子;丁腈和烯烃增加了化合物的极化性,从而增强了粘附分子的弱分子间引力,如氢键和范德华力,最终增加了所产生的动物胶的粘附强度和内聚力。
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引用次数: 0
Identification, antimicrobial susceptibility screening and ESBL-status of Gram-negative bacteria from healthy humans and livestock waste by VITEK-2 Automated System VITEK-2系统对健康人畜粪便中革兰氏阴性菌的鉴定、药敏筛选及esbl状态分析
Pub Date : 2022-09-08 DOI: 10.4314/sa.v21i2.8
E. Nmema, C. Osuagwu, E. A. Tobin
Bacteria in healthy body sites of humans and livestock waste may harbour antibiotic resistance and cause community-based opportunistic and resistant infections. The study profiled the antibiotic susceptibilities of resident bacteria in healthy humans and livestock waste. Gram-negative bacteria were isolated from 23 specimens including skin swabs (6), nasal swabs (4), urine (6), stool (3), chicken droppings (2) and cattle droppings (2). VITEK® 2 Automated System was used for identification, antimicrobial susceptibility and extended-spectrum β-lactamase (ESBL) production test of the isolates. Nineteen (19) Gram-negative bacteria belonging to five genera and six species were identified, including Escherichia coli (n=9) 47.4%, Klebsiella pneumoniae ssp pneumoniae (n=1) 11.1%, Enterobacter cloacae ssp dissolvens (n=1) 11.1%, Acinetobacter baumannii (n=3) 15.8%, Acinetobacter haemolyticus (n=1) 11.1%, and Providentia stuartii (n=4) 21.1%. The isolates showed highest resistances to Ampicillin (78.6%) and Piperacillin (63.2%) and high susceptibilities to Ertapenem, Amikacin, Ciprofloxacin and Levofloxacin (100%); Ceftazidime, Cefepime, Meropenem (94.7%); Cefoxitin (93.3%); Gentamicin and Tobramycin (73.7%). Multiple Antibiotic Resistance (MAR) index values above the critical limit of 0.2 were shown by 100% (4/4) of Providentia stuartii isolates, 75% (3/4) of Acinetobacter isolates and 33.3% (3/9) of E. coli isolates. All the isolates tested negative for ESBL production. The public health implication is that resident bacteria from healthy individuals harbouring antibiotic resistance may transmit these to other bacteria or cause resistant opportunistic infections difficult to treat. Resistant bacteria from livestock can be transmitted to humans through the food chain. Proper disposal or decontamination of human body secretions and livestock waste is necessary.
人类健康身体部位和牲畜粪便中的细菌可能含有抗生素耐药性,并引起基于社区的机会性和耐药感染。该研究描述了健康人类和牲畜粪便中常驻细菌对抗生素的敏感性。从皮肤拭子(6)、鼻拭子(4)、尿液(6)、粪便(3)、鸡粪(2)和牛粪(2)等23份标本中分离到革兰氏阴性菌。采用VITEK®2全自动系统对分离株进行鉴定、药敏试验和广谱β-内酰胺酶(ESBL)产酶试验。共检出革兰氏阴性菌19株,隶属5属6种,其中大肠埃希菌(9株)47.4%,肺炎克雷伯菌(1株)11.1%,阴沟肠杆菌(1株)11.1%,鲍曼不动杆菌(3株)15.8%,溶血不动杆菌(1株)11.1%,斯氏普罗维登斯菌(4株)21.1%。对氨苄西林(78.6%)和哌拉西林(63.2%)的耐药率最高,对厄他培南、阿米卡星、环丙沙星和左氧氟沙星的敏感性最高(100%);头孢他啶、头孢吡肟、美罗培南(94.7%);头孢西丁(93.3%);庆大霉素和妥布霉素(73.7%)。其中,100%(4/4)的斯达罗维登氏菌、75%(3/4)的不动杆菌和33.3%(3/9)的大肠杆菌的多重耐药指数均高于临界限值0.2。所有分离株ESBL检测均为阴性。这对公共卫生的影响是,来自健康个体的具有抗生素耐药性的常驻细菌可能将这些细菌传播给其他细菌或导致难以治疗的耐药机会性感染。来自牲畜的耐药细菌可通过食物链传播给人类。必须妥善处理或净化人体分泌物及禽畜废物。
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引用次数: 0
Intelligent rule-based system for diagnosing eye diseases 基于规则的智能眼病诊断系统
Pub Date : 2022-09-08 DOI: 10.4314/sa.v21i2.13
E.S. Kobani, U. Okengwu
This topic presents the development of an intelligent rule-based system for diagnosing multiple Eye diseases. The motivation behind this work was due to the inflexibility of the existing systems that are only able to diagnose a single type of eye disease. An expert system like the CASNET/GLAUCOMA or PADI-HAA was used to diagnose glaucoma. The stages used to develop our system includes modelling, Knowledge representation, Knowledge acquisition, Inference and System evaluation. This system was developed using HTML, CSS and JavaScript for the frontend and Node.JS for the backend. This system can diagnose eleven different eye diseases thereby solving the issue of the inflexibility of other expert systems.
本课题介绍了一种基于规则的多种眼病智能诊断系统的开发。这项工作背后的动机是由于现有系统的不灵活性,只能诊断单一类型的眼病。采用CASNET/GLAUCOMA或PADI-HAA等专家系统诊断青光眼。该系统的开发阶段包括建模、知识表示、知识获取、推理和系统评估。本系统以HTML、CSS和JavaScript为前端,以Node.JS为后端进行开发。该系统可以诊断11种不同的眼病,从而解决了其他专家系统缺乏灵活性的问题。
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引用次数: 0
Lithofacies control on depositional environments in shallow offshore Niger Delta: implication on reservoir quality 尼日尔三角洲浅海沉积环境的岩相控制:对储层质量的启示
Pub Date : 2022-09-08 DOI: 10.4314/sa.v21i2.7
O. Ndukwe, G. Udom, C. Ugwueze
The KEN field lies within latitude 4⁰52ꞌ44ꞌꞌ N to 4⁰53'04'' N and 6⁰22'50'' E to 6⁰22'26'' E. The geological and geophysical data sets were used to describe reservoir depositional facies and their environment of deposition. A detailed and accurate environment of sediment delineation is a solid basis to enhance characterization and providing measures for improving hydrocarbon reservoirs. This study aims to presents an effective method for accurately defining depositional environment with different data sets. The data used comprises of biostratigraphy, well logs from three wells and 3D full angle stack seismic data. The biostratigraphic data help to ascertain the age of the formation delineated to be middle Miocene to late Miocene based on the marker shale. It also helps in picking the stratigraphic surfaces. Three depositional sequences were delineated and dated with maximum flooding surfaces of 15.0, 12.6 and 11.5 Ma, respectively. Log sequence analysis reveals the internal geometry and stacking pattern of the mapped sequences. The gamma ray signature varies from serrated cylindrical, funnel to the bell-shaped log motif. The seismic stratigraphy involved facies analysis and reflection termination patterns, which aided the mapping of depositional sequence. The internal geometry is composed of Highstand, transgressive, and lowstand systems tracts. The stacking patterns vary from progradational, aggradational and retrogradational. Based on the seismic facies analysis, the integrated results show that the field of study is of pelagic and debris flow origin deposited in shallow marine settings, which also conform with the other data sets used for this study. The depositional environment of the three delineated reservoirs (Reservoir A, B and C) vary from deltaic upper to lower shoreface channels sand. Reservoir C, which is laterally continuous across the three studied wells shows that the connectivity of reservoir C is loosely amalgamated. The integrated data used for this study indicate that the environment of deposition varies from inner neritic to outer neritic environment. The results of this research are essential for reservoir quality, exploration, appraisal and development phases.
KEN油田位于北纬4⁰52′44′N至北纬4⁰53′04′N和东经6⁰22′50′E至东经6⁰22′26′E之间,地质和地球物理数据集用于描述储层沉积相及其沉积环境。详细、准确的沉积环境圈定是加强油气藏表征和提供改造措施的坚实基础。本研究旨在提出一种利用不同数据集准确定义沉积环境的有效方法。所使用的数据包括生物地层学、三口井的测井曲线和三维全角度叠置地震数据。生物地层资料有助于根据标志页岩圈定的中新世中期至中新世晚期地层。它还有助于选择地层表面。圈定了3个沉积层序,最大淹没面分别为15.0、12.6和11.5 Ma。测井层序分析揭示了映射层序的内部几何结构和叠加规律。伽马射线特征从锯齿状圆柱形、漏斗形到钟形原木图案不等。地震地层学涉及相分析和反射终止模式,有助于沉积层序的填图。内部几何结构由高水位、海侵和低水位体系域组成。堆积模式有递进型、递进型和倒退型。基于地震相分析,综合结果表明研究区属于浅海背景下的远洋和碎屑流成因,这与本研究的其他数据集一致。圈定的3个储层(A、B、C)的沉积环境不同于三角洲上、下滨面河道砂体。C储层横向连续分布在3口研究井中,表明C储层的连通性是松散混合的。综合资料表明,沉积环境由内浅海环境向外浅海环境变化。研究结果对储层质量、勘探、评价和开发阶段具有重要意义。
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引用次数: 0
Comparative study of the proximate compositions of some scaled and unscaled fish 一些有鳞和无鳞鱼类的近似成分的比较研究
Pub Date : 2022-09-08 DOI: 10.4314/sa.v21i2.6
K. U. Ogbe, O. Omada, E. Dasuma
A study on the proximate composition of 4 scaled and unscaled fish samples were carried out in September, 2019 using standard procedures. The protein content between 7.02±2.05% in the scaled fish samples, while that of unscaled fish ranged from 18.29±2.26%. Concentrations of fats were 2.21±0.70% in scaled while unsealed were 2.58±21.10%. Fibre values ranged from 0.130±0.0.037% in the scaled and unscaled were observed to be 0.26±0.11%. Carbohydrate recorded in scaled fish samples ranged from 12.03±5.65 in unscaled fish samples ranged from 9.71±2.30% . Ash content observed was (0.61±0.36% in scaled fish samples while unscaled fish samples shows 1.93±0.69% Moisture contents recorded in scaled was 67.74±5.17% while unscaled shows 67.18±3.73%. They was no significance difference at P > 0.05 except for ash content. Bargus bayad had the highest crude protein content and could be recommended as a possible effective way to solve protein malnutrition
2019年9月,采用标准程序对4种有鳞和无鳞鱼类样本的近似组成进行了研究。有鳞鱼的蛋白质含量为7.02±2.05%,无鳞鱼的蛋白质含量为18.29±2.26%。密封后脂肪浓度为2.21±0.70%,未密封时脂肪浓度为2.58±21.10%。纤维值范围为0.130±0.0.037%,其中有鳞和未鳞的纤维值为0.26±0.11%。有鳞鱼的碳水化合物含量为12.03±5.65%,无鳞鱼的碳水化合物含量为9.71±2.30%。有鳞鱼的灰分含量为(0.61±0.36%),无鳞鱼的灰分含量为(1.93±0.69%)。有鳞鱼的水分含量为67.74±5.17%,无鳞鱼的水分含量为67.18±3.73%。除灰分含量外,其余各组间无显著差异P > 0.05。鲈鱼粗蛋白质含量最高,可作为解决蛋白质营养不良的有效途径
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引用次数: 0
Health hazards and risk assessment of the use of fossil fuel electricity generators in suburb of Port Harcourt, Nigeria 尼日利亚哈科特港郊区使用化石燃料发电机的健康危害和风险评估
Pub Date : 2022-09-08 DOI: 10.4314/sa.v21i2.2
C. I. Osu, B. Ordinioha, Port Harcourt. P.M.B. 5323
Study on the health hazards and risk assessment of the use of fossil fuel electricity generators in suburb of Port Harcourt, Nigeria was carried out. The results showed that the concentrations of the metals varied greatly among the vegetable species and locations. It was observed that the vegetables (Telfairia occidentalis and Talinum triangulare) cultivated less than 15m away from fossil fuel generator after analysis showed some level of trace metals concentration. The variation in metal concentrations in the vegetables can be attributed to the metal concentrations in soils. The concentration of the metals in the edible vegetables studied ranged from 0.90 ± 0.01 to 3.20 ± 0.001, Pb; 0.08 ± 0.001 to 0.11 ± 0.01, Cd; 53.10 ± 0.002 to 106.30 ± 0.02, Fe; and 0.40 ± 0.002 to 1.60 ± 0.02, Zn. The difference in the concentration of the metals in the various vegetable can be attributed to the different concentration of the metals in the soils and also the direction at which the vegetation was located. The CDI values of iron for adult and children were all above oral reference dose (RfD) (0.007mg/kg/day). The hazard quotient (HQ) values of the metals in all the stations were greater than 1 except Zn and Cu for children. Lead, Iron, Cadmium and Chromium exceeded 1 for both adult and children while in In all the stations, HQ values were generally high for children, thereby making people living around the area more vulnerable. The high HQ values recorded in this study were as result of high CDI values of the metals involved. These metals pose long term health risk to the land users. All the hazard index (HI) values recorded in this study were well above 1. The long-term health risk is high and the non-carcinogenic adverse effect is not negligible. Health risk assessment for all the sites considered showed that cumulative effect of some of the heavy metal studied, as indicated by the hazard index (HI), calls for concern.
对尼日利亚哈科特港郊区使用化石燃料发电机的健康危害和风险评估进行了研究。结果表明,不同蔬菜种类和地点的重金属含量差异较大。分析发现,在距离化石燃料发电机15m范围内种植的蔬菜(西部泰利亚和三角泰利)中,痕量金属含量较高。蔬菜中金属含量的变化可归因于土壤中金属含量的变化。所研究的食用蔬菜中金属的浓度范围为0.90±0.01 ~ 3.20±0.001 Pb;0.08±0.001 ~ 0.11±0.01,Cd;53.10±0.002 ~ 106.30±0.02,Fe;0.40±0.002 ~ 1.60±0.02,Zn。不同蔬菜中金属含量的差异可归因于土壤中金属含量的不同以及植物所处的方向。成人和儿童铁的CDI值均高于口服参考剂量(RfD) (0.007mg/kg/d)。除儿童Zn和Cu外,各监测站重金属的危害商(HQ)值均大于1。成人和儿童的铅、铁、镉和铬含量均超过1,而在所有监测站中,儿童的HQ值普遍较高,从而使生活在该地区的人们更加脆弱。本研究中记录的高HQ值是由于所涉及金属的高CDI值。这些金属对土地使用者的健康构成长期风险。本研究记录的所有危害指数(HI)值均大于1。长期健康风险高,非致癌性不良影响不容忽视。对所有考虑的地点进行的健康风险评估表明,危害指数(HI)表明,所研究的某些重金属的累积效应值得关注。
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引用次数: 0
Monitoring hydrocarbon levels during bioremediation by enhanced bio-stimulants using GC-FID 利用气相色谱- fid监测强化生物刺激素在生物修复过程中的碳氢化合物水平
Pub Date : 2022-09-08 DOI: 10.4314/sa.v21i2.14
E. Ezeani, N. Ngobiri, I. Agbagwa
The efficacy of bio-stimulants on the remediation of hydrocarbon polluted soil was assessed using Gas Chromatography with Flame Ionization Detector (GC-FID). The enhanced bio-stimulants were discarded melon pulps, discarded breadfruit pulps and poultry droppings. These were applied to the polluted soil in the ratio of 1:5; the blend was observed for sixty days. Inferring from the chromatographs, the carbon compounds present in the polluted and remediated soils ranged from C12 – C40 with varying concentrations; C12 to C19 were dominant, C9 to C11 were residual with negligible concentrations. The total petroleum hydrocarbon (TPH) of polluted soil was 42,229.73 mg/kg, and the remediated soils were: biodegraded melon pulp - 23,786.3 mg/kg, biodegraded breadfruit pulp - 15,322.82 mg/kg, and chicken droppings, - 7,314.29 mg/kg. The results indicated that TPH of the polluted soil was reduced by 43.67% in sample remediated with biodegraded melon pulp, 63.71% in sample remediated with biodegraded breadfruit pulp, and 82.67% in sample remediated with chicken droppings. Therefore, a decreasing order of the effectiveness of the bio-stimulants is thus: chicken droppings > biodegraded breadfruit pulp > biodegraded melon pulp. The higher remediation potential of poultry droppings is attributable to high nitrogenous content. The study showed that the aforementioned bio-stimulants are effective in remediation of petroleum polluted soil. GC-FID detected the hydrocarbon present and their concentrations in the polluted and remediated soils. GC-FID is preferred to other analytical techniques due to its precision in identification and quantification of hydrocarbon fractions.
采用气相色谱-火焰离子化检测器(GC-FID)评价了生物刺激剂对烃类污染土壤的修复效果。强化的生物刺激物是废弃的甜瓜果肉、废弃的面包果果肉和家禽粪便。按1:5的比例施用于污染土壤;观察共混物60 d。从色谱分析可以看出,污染土壤和修复土壤中的碳化合物含量在C12 ~ C40之间,且浓度不同;C12 ~ C19为主要成分,C9 ~ C11为残余,浓度可忽略不计。污染土壤的总石油烃(TPH)为42,229.73 mg/kg,修复土壤为:生物降解甜瓜果肉- 23,786.3 mg/kg,生物降解面包果果肉- 15,322.82 mg/kg,鸡粪- 7,314.29 mg/kg。结果表明,生物降解甜瓜浆修复的土壤TPH降低43.67%,生物降解面包果浆修复的土壤TPH降低63.71%,鸡粪修复的土壤TPH降低82.67%。因此,生物刺激剂的有效性降序为:鸡粪b>生物降解面包果果肉>生物降解甜瓜果肉。家禽粪便具有较高的修复潜力是由于其含氮量高。研究表明,上述生物刺激剂对石油污染土壤的修复是有效的。气相色谱- fid检测了污染土壤和修复土壤中存在的碳氢化合物及其浓度。气相色谱- fid在烃类组分的鉴别和定量方面具有较高的精度,是其他分析技术的首选。
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引用次数: 0
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Scientia Africana
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