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Effect of Various Culture Parameters on the Bio-surfactant Production from Bacterial Isolates 不同培养参数对细菌分离物生产生物表面活性剂的影响
Pub Date : 2017-12-22 DOI: 10.4172/2157-7463.1000350
A. Roy
Biosurfactants are those compounds which contains both hydrophilic and hydrophobic moieties i.e., amphiphilic in nature. They have advantages over the chemical surfactant such as low toxicity, eco-friendly, etc. They possess wide range of industrial applications such as bioremediation, food processing, oil industries and health care. Various microorganisms have the ability to produce the potential biosurfactant. In the present study, microbial culture which showed highest biosurfactant activity i.e., SA3 based on the oil displacement activity and emulsion layer was selected for various culture parameters optimization. Sodium nitrate as a nitrogen source and dextrose as a carbon gives the highest biomass and biosurfactant production. Further 2% of dextrose and 2% of inoculum size provides the highest biomass and biosurfactant production. Growth kinetics and biosurfactant kinetics study reveals that the maximum biomass was produced after 96 h and maximum production of biosurfactant was after 72 h of incubation period. Overall, the results indicated the potential use of SA3 isolate in bioremediation processes.
生物表面活性剂是那些同时含有亲水性和疏水性基团的化合物,即本质上是两亲性的。与化学表面活性剂相比,具有低毒、环保等优点。它们具有广泛的工业应用,如生物修复,食品加工,石油工业和医疗保健。各种微生物都有能力产生潜在的生物表面活性剂。本研究选取生物表面活性剂活性最高的微生物培养物,即基于驱油活性和乳化液层数的微生物培养物SA3进行各项培养参数优化。硝酸钠作为氮源,葡萄糖作为碳源,生物量和生物表面活性剂产量最高。另外,2%的葡萄糖和2%的接种量可提供最高的生物量和生物表面活性剂产量。生长动力学和生物表面活性剂动力学研究表明,培养96 h后生物量最大,培养72 h后生物表面活性剂产量最大。总的来说,结果表明SA3分离物在生物修复过程中的潜在应用。
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引用次数: 4
Detection and Quantification of Sulfate-Reducing and Polycyclic Aromatic Hydrocarbon-Degrading Bacteria in Oilfield Using Functional Markers and Quantitative PCR 油田硫酸盐还原和多环芳烃降解细菌的功能标记和定量PCR检测与定量
Pub Date : 2017-11-27 DOI: 10.4172/2157-7463.1000348
B. Nasser, Ramadan Ar, H. Ry, Mohamed Me, W. Ismail
Oilfield water samples from injection water treatment facility and soil/sludge samples from Gas Oil Separation Plant (GOSP) at Saudi Aramco were analyzed for the presence of SRB and PAH-degrading bacteria. SRB were detected by targeting a fragment of the apsA gene encoding adenosine-5-phosphosulfate reductase, which is characteristic of all SRB. The PAH-degrading bacteria were detected using a primer pair that amplifies a fragment of the gene encoding the large subunit of the naphthalene dioxygenase gene nahA. The nahA gene was detected in almost half of the soil/sludge samples with the highest copy number of 60540 copies/g soil/sludge. Most of the analyzed water samples contained high copy numbers of nahA gene with the highest copy number 3846 copies/ml. Most of the analyzed water samples revealed the presence of high copy numbers of the apsA gene with the highest copy number of 44 x 106/ml in sample number 2. Only 7 of the soil/sludge samples revealed the presence of the apsA gene with the highest copy number of 107920/g soil/sludge in sample number 11. In contrast to the nahA gene, the highest copy numbers of the apsA gene were detected in the water samples. SRB and PAH-degrading bacteria exist in some Saudi oilfields and appear to play a role in the H2S production and PAH degradation.
对沙特阿美公司注水处理设施的油田水样和油气分离厂(GOSP)的土壤/污泥样品进行了SRB和多环芳烃降解细菌的分析。通过靶向编码腺苷-5-磷酸硫酸还原酶的apsA基因片段检测SRB,这是所有SRB的特征。使用扩增编码萘双加氧酶基因nahA大亚基的基因片段的引物对检测多环芳烃降解细菌。在几乎一半的土壤/污泥样品中检测到nahA基因,最高拷贝数为60540拷贝/g土壤/污泥。大部分分析水样nahA基因拷贝数较高,最高拷贝数为3846拷贝/ml。大部分水样均存在高拷贝数的apsA基因,拷贝数最高的水样2为44 × 106/ml。只有7份土壤/污泥样品存在apsA基因,11号样品中apsA基因拷贝数最高,为107920/g土壤/污泥。与nahA基因相比,apsA基因在水样中拷贝数最高。SRB和多环芳烃降解细菌存在于沙特的一些油田中,并在H2S的产生和多环芳烃的降解中发挥作用。
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引用次数: 3
Analytical Decline Curve Analysis Model for Water Drive Gas Reservoirs 水驱气藏递减曲线分析模型
Pub Date : 2017-10-16 DOI: 10.4172/2157-7463.1000343
M. Abdelkhalek, Ahmed H. El-Banbi, M. Sayyouh
Production data analysis is a viable tool for reservoir characterization and estimation of initial gas in place (IGIP) and reserves. Several methods are available to analyse production data starting with Arps classical decline curve analysis (DCA) in 1945 all the way to more sophisticated analytical and advanced DCA techniques. Most of these methods are applicable only for single phase flow in porous media. In this paper, we present a simple analytical decline curve analysis (ADCA) model that takes into account the effect of water influx on gas reservoir performance. We introduced the water influx effect into the pseudo-steady state flow equation which enables us to estimate the reservoir pressure and the IGIP for water drive gas reservoirs. The model is based on coupling the material balance equation for gas reservoirs, aquifer models, and the gas flow equation to calculate the well’s production rate versus time. The model can also estimate reservoir pressure, gas saturation, water production rate, and gas production rate with time. When the model is run in history-match mode to match gas and water production, we can estimate the IGIP, well’s productivity index, and aquifer parameters. The model can also be run in prediction mode to predict gas and water production at any conditions of bottom-hole flowing pressure (BHFP) (or surface tubing pressure) and reserves can be calculated. The model was validated with several simulated cases at variable conditions of rate and pressure. The model was then used to perform decline curve analysis in several field cases. This technique is fast and requires minimum input data. The paper will also present the application of this technique to analyse production data and predict reserves for gas wells producing both gas and water.
生产数据分析是储层表征和估算初始含气量(IGIP)和储量的可行工具。有几种方法可以用于分析生产数据,从1945年Arps的经典递减曲线分析(DCA)开始,一直到更复杂的分析和先进的DCA技术。这些方法大多只适用于多孔介质中的单相流动。本文提出了考虑水侵量对气藏动态影响的简单递减曲线分析(ADCA)模型。在拟稳态渗流方程中引入水侵效应,可以估算水驱气藏的储层压力和IGIP。该模型基于气藏的物质平衡方程、含水层模型和气体流动方程的耦合,以计算井的产量随时间的变化。该模型还可以估计油藏压力、含气饱和度、产水速率和产气速率随时间的变化。当模型以历史匹配模式运行以匹配气、水产量时,我们可以估计出IGIP、井的产能指数和含水层参数。该模型也可以在预测模式下运行,预测任何井底流动压力(BHFP)(或地面油管压力)条件下的产气和产水,并计算储量。在变速率和变压力条件下对模型进行了验证。然后将该模型用于几个油田实例的递减曲线分析。这种技术速度快,并且需要最少的输入数据。本文还将介绍该技术在既有气又有水的气井生产数据分析和储量预测中的应用。
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引用次数: 0
Preparation of Sorbitol Palmitate by Organic Catalysis and Its Application for Base Oil Stabilization 有机催化制备山梨糖醇棕榈酸酯及其在基础油稳定中的应用
Pub Date : 2017-08-31 DOI: 10.4172/2157-7463.1000339
N. El-Mehbad
Esters are excellent lubricants and high performance industrial fluids, but they are often costly. We prepared the ester sorbitol palmitate via an inexpensive phase-transfer catalysis method as an additive for the retardation of oil oxidation. The effects of the sorbitan palmitate content on the lubricant properties and oxidation stability of a base oil were determined. The addition of sorbitan palmitate to the oil retarded oxidation and enhanced the pour point depression. A novel method for inhibiting oxidation through the action of micellar cores was suggested. This micellar inhibition offers a new concept for the protection of lubricants against oxidative degradation.
酯类是优良的润滑剂和高性能工业流体,但它们通常价格昂贵。采用廉价的相转移催化法制备了山梨糖醇棕榈酸酯,并将其作为延缓油脂氧化的添加剂。研究了山梨糖棕榈酸酯含量对基础油润滑性能和氧化稳定性的影响。山梨糖棕榈酸酯的加入延缓了氧化,增强了降凝点。提出了一种利用胶束核抑制氧化的新方法。这种胶束抑制为润滑油抗氧化降解提供了一种新的保护概念。
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引用次数: 0
Geologic Evaluation of Kirkuk Underground Storage Project 基尔库克地下储库工程地质评价
Pub Date : 2017-08-31 DOI: 10.4172/2157-7463.1000340
Al-Sulaiman Fa, Fadaws Ah, A. Ta
Geologic evaluation of the Kirkuk underground was conducted by comparison of the geometric properties of the cavities derived from two surveys running during 1989 and 2015. The vertical cross sections of the cavities show that most cavities have shown that most cavities have irregular shape because of the presence of impurities in the salt bed that cause different leaching velocities in different directions. The relationship of the cavity roof with tilted salt bed was studied, that shows some of the cavities are not safe to store LPG.
基尔库克地下的地质评估是通过比较1989年和2015年两次调查得出的洞穴的几何特性来进行的。由于盐层中杂质的存在,导致不同方向的浸出速度不同,因此大多数空腔具有不规则的形状。研究了岩洞顶板与倾斜盐层的关系,表明部分岩洞不安全。
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引用次数: 0
Bulk Physiognomies and Sara Constituents of Bituminous Sands from Ondo State, Nigeria 尼日利亚翁多州沥青砂的整体地貌和主要成分
Pub Date : 2017-08-31 DOI: 10.4172/2157-7463.1000338
V. Co, Osuji Lc, Onojake Mc
Bituminous sands obtained from six different locations in Ondo State, Nigeria were analysed. Physicochemical parameters were studied using American Standard Testing and Materials (ASTM) procedures and analysis of hydrocarbon and non-hydrocarbon fractions using Ultraviolet Visible Spectrophotometer were undertaken. Results obtained show: density (0.877-0.884 g/cm3); specific gravity (0.878-0.886); API gravity (28.133°API to 29.531°API); kinematic viscosity (0.440 cSt to 0.550 cSt); dynamic viscosity (0.290-0.360); cloud point (-15°C to -2°C); moisture content (775 ppm to 1761 ppm); gum content (605-1895 mg/100 ml) and pour point of <-34°C. Result of the Saturates, Aromatics, Resins and Asphaltene (SARA) analysis revealed; saturates; 44.139% to 69.436%, aromatics; 21.778% to 44.949%, resins; 11.067% to 28.369% and asphaltenes; 8.634% to 29.278%. They suggest that these bituminous sands have similar characteristics, can be classified as medium heavy oils on the API gravity scale, high in saturate hydrocarbons and low in asphaltene content which indicates their high hydrocarbon potential.
分析了从尼日利亚翁多州六个不同地点获得的沥青砂。采用ASTM (American Standard Testing and Materials)程序研究了理化参数,并采用紫外可见分光光度计对烃类和非烃类组分进行了分析。结果表明:密度(0.877 ~ 0.884 g/cm3);比重(0.878-0.886);API重力(28.133°API至29.531°API);运动粘度(0.440 cSt至0.550 cSt);动态粘度(0.290-0.360);浊点(-15℃至-2℃);水分含量(775 PPM至1761 PPM);胶含量(605-1895 mg/100 ml),倾点<-34°C。饱和烃、芳烃、树脂和沥青质(SARA)分析结果使充满;芳烃44.139% ~ 69.436%;21.778% ~ 44.949%,树脂;11.067% ~ 28.369%沥青质;8.634%至29.278%。这些沥青砂具有相似的特征,在API比重尺度上可归类为中稠油,饱和烃含量高,沥青质含量低,具有较高的成烃潜力。
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引用次数: 2
Quick Opening Closures-A New Stage in Petrochemical Industry 快开闭——石化工业发展的新阶段
Pub Date : 2017-08-17 DOI: 10.4172/2157-7463.1000335
I. Khasanov
At new construction and reconstruction of petrochemical production facilities for replacement of bolted equipment covers and hatches are widely used quick opening closures of different design. In this paper are analyzed quick opening closures of covers and hatches as well as pipelines, chambers and apparatuses dead end sections. Their advantages and shortcomings are presented. It is shown that high reliability, fabricability and ease of use requirements are met by new generation quick opening closures–ZKSsh/Zatvor Kontsevoi Sektorny Sheryk=Sector End Closure by Sherik. The closure design makes it possible to fabricate and deliver it both as an equipment component and in the form of a separate finished product to be installed at various vertically or horizontally oriented branch pipes, including in replacement of the existing fixtures in all possible diameter and pressure ranges.
在新建和改造的石化生产设施中,用于更换螺栓式设备的盖板和舱口广泛采用不同设计的快开闭件。本文分析了井盖、舱口、管道、腔室和设备死角段的快开闭。介绍了它们的优缺点。这表明,高可靠性,可制造性和易用性的要求是满足新一代快速开启关闭- zkssh /Zatvor Kontsevoi Sektorny Sheryk=扇区结束关闭由Sherik。封闭设计使得它既可以作为设备组件制造和交付,也可以作为单独的成品安装在各种垂直或水平方向的分支管道上,包括在所有可能的直径和压力范围内替换现有的夹具。
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引用次数: 0
A Treatise on Non-Darcy Flow Correlations in Porous Media 多孔介质中非达西流动关系的研究
Pub Date : 2017-08-12 DOI: 10.4172/2157-7463.1000336
Vaishali Sharma, A. Sircar, N. Mohammad, S. Patel
Non-Darcy Flow behavior is important for describing fluid flow in consolidated or unconsolidated porous media when abrupt changes in velocity dominates. A criterion or a generalized equation is required to understand this flow behavior in the isotropic/anisotropic carbonate and sandstone reservoirs, and naturally or hydraulically fractured reservoirs. Various correlations and equations have been reviewed in this paper to quantify this non-Darcy coefficient (i.e., beta coefficient) mathematically. It has been observed that this coefficient is highly dependent on rock properties (mainly porosity, permeability and tortuosity). An algorithm to determine the values of the beta coefficient by using the correlations have been presented and coded and converted in to a robust user-friendly simulator. This simulator can take a large amount of data set as input and will generate a large data set of beta values as output. The obtained or calculated beta value is very useful for predicting the change in pressure gradient with respect to velocity and hence can give the best estimate of hydrocarbon production under challenging or adverse pressure drop conditions.
非达西流动特性对于描述以速度突变为主的固结或松散多孔介质中的流体流动具有重要意义。在各向同性/各向异性的碳酸盐岩和砂岩储层以及自然或水力裂缝储层中,需要一个标准或广义方程来理解这种流动行为。本文回顾了各种相关性和方程,以数学方式量化这种非达西系数(即β系数)。已经观察到,该系数高度依赖于岩石性质(主要是孔隙度、渗透率和弯曲度)。提出了一种利用相关性确定β系数值的算法,并对其进行了编码,并将其转换为鲁棒的用户友好模拟器。该模拟器可以将大量的数据集作为输入,并将生成大量的beta值数据集作为输出。获得或计算的beta值对于预测压力梯度相对于速度的变化非常有用,因此可以在具有挑战性或不利的压降条件下对油气产量进行最佳估计。
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引用次数: 4
Research and Practice of the Early Stage Polymer Flooding on LD Offshore Oilfield LD海上油田早期聚合物驱研究与实践
Pub Date : 2017-08-07 DOI: 10.4172/2157-7463.1000337
Kuiqian Ma, Yanlai Li, Ting Sun
Literature survey shows that polymer flooding was generally conducted during high water-cut stage (WCT>80% to 90%). Even the first China Offshore polymer flooding project was carried out in SZ when water cut was 60%. By then, conduction of polymer flooding in early phase (WCT<10%) was just discussed in theory. For offshore oilfield, the treatment of water could be costly. Because polymer improves mobility ratio of replacement fluid over oil and sweep efficiency, less water is injected and less water is produced. So, we did enormous research about the polymer flooding on early stage by theoretical analysis, series of experiments and chemical flooding simulation. Based on these researches, we carried out the first field test of polymer flooding on early stage in LD. Single well polymer injection test was started in Mar 2006 when the water cut in the pattern was lower than 10%. After the trial, there were other 5 water injectors being converted to polymer injectors from 2007 to 2009. The polymer flooding controlled reserve was about 25,250,000 m3. For the early stage polymer flooding, the characteristics of the responses on producers were different from the case in which polymer flooding was conducted during high water cut stage. The water producing of the producers continued to rise up after polymer flooding, but the simulation research showed that the water cut increasing rate was lower than the rate during merely water flooding. In addition, we observed the drop-down on the water cut in some wells, such as A11, A12, A13, A15, etc. For the well A11, the highest water cut reduction reached 41% after the injectors (A5/A10) profiles controlled, and net incremental oil for A11 even reached 154,510 m3. By Dec 2014, the total incremental oil by polymer flooding was about 754,650 m3, and the stage oil recovery efficiency was enhanced by 3.0%. The polymer flooding is still effective, and we will get more oil from the polymer flooding.
文献调查表明,聚合物驱一般在高含水阶段(WCT>80% ~ 90%)进行。即使是中国海上的第一个聚合物驱项目,也是在深圳进行的,含水率为60%。此时,对早期(WCT<10%)聚合物驱的导流问题进行了理论探讨。对于近海油田来说,水的处理成本可能很高。由于聚合物提高了替代液对油的流动性比和波及效率,因此注入的水更少,出水量也更少。为此,我们从理论分析、一系列实验和化学驱模拟等方面对聚合物驱进行了前期的大量研究。在此基础上,开展了该区块早期聚合物驱的首次现场试验,并于2006年3月在该区块含水率低于10%的情况下开始了单井注聚合物试验。试验结束后,从2007年到2009年,另有5个注水井被改造成聚合物注水井。聚合物驱控制储量约2525万m3。在聚合物驱早期,采油者的响应特征与高含水阶段的聚合物驱不同。聚合物驱后,采油者的产水量持续上升,但模拟研究表明,含水增加速率低于单纯水驱时的含水增加速率。此外,A11、A12、A13、A15等井的含水率也出现了下拉现象。对于A11井,在注入器(A5/A10)剖面控制后,最高含水率降低了41%,A11井的净增油量甚至达到了154,510 m3。截至2014年12月,聚合物驱累计增油量约754,650 m3,阶段采收率提高3.0%。聚合物驱仍然是有效的,我们将从聚合物驱中获得更多的油。
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引用次数: 1
Health, safety and risk within the Kuwait Oil Company context 科威特石油公司范围内的健康、安全和风险
Pub Date : 2017-07-27 DOI: 10.4172/2157-7463-C1-034
Manal Hamad AlAmiri
P of CO2 and hydrocarbon (HC) gas injection into a heavy crude oil was investigated at high pressure/temperature condition, using high permeable well-sorted original reservoir sandstone. Complete series of PVT and slime tube tests were followed by vertical and horizontal gas floods to study the impact of injection rate, injectant type and reservoir pressure. Dimensional analysis was performed to study the involved mechanism and forces. Sometimes direct injection of CO2 may not be practically and economically possible. In addition, in plans for CO2 storage, CO2 as a free phase in a reservoir is coupled with a significant leakage risk that prevents the scenario of direct injection. Therefore, in the second part, the enhancement of heavy oil recovery was tested by the carbonated water injection. The results of the first part of core flooding experiments demonstrated that gravity and solubility are the most effective mechanisms in oil recovery. The reduction in oil recovery in horizontal flooding for HC gas injection is higher due to the smaller difference between the densities of CO2 and oil compared to HC gas/oil systems. Furthermore, a small increase of oil recovery after breakthrough (BT) during N2 injection proves the importance of the solubility mechanism. Therefore, In this case, more precise analysis could be performed by applying the dissolution number instead of capillary and/or bond number. For the second part of the experiments, the results obtained demonstrate that the capability of carbonated water to enhance oil recovery for both secondary and tertiary flooding is significantly greater than that for water flooding. The creation of a low resistance flow channel and low oil recovery in water flooding is compensated by CO2 diffusion and subsequent viscosity reduction and oil swelling in heavy oils.
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引用次数: 1
期刊
Journal of Petroleum & Environmental Biotechnology
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