Journal of periodontology最新文献_第3页

Mediating role of immune cells in association between volatile organic compounds and periodontitis: NHANES 2011–2014 免疫细胞在挥发性有机化合物与牙周炎相关中的介导作用：NHANES 2011-2014

IF 4.3 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-12-18 DOI: 10.1002/jper.24-0150

Wenxiu Jiang, Wei Wu, Kejia Zhang, Luwei Liu, Bin Yan

BackgroundThe relationship between humans and volatile organic compounds (VOCs) is a persistent concern due to their widespread sources and high evaporation rates. However, there is currently limited direct evidence linking VOC exposure to the development of periodontitis.MethodsThis cross‐sectional study analyzed 1525 participants and 21 urinary VOCs in the National Health and Nutrition Examination Survey (NHANES) from 2011 to 2014, aiming to investigate the relationship between periodontitis risk, assessed by attachment loss (AL) and probing depth (PD) and individual VOCs using logistic regression, quantile regression, and subgroup analysis. Weighted quantile sum analysis (WQS) and subgroup analysis were utilized to evaluate whether VOC mixtures were associated with periodontitis risk. Multiple linear regression models were used to examine the association between VOC co‐exposure and peripheral immune cell counts. A mediation analysis was performed to evaluate whether peripheral immune cells are involved in the effect of VOC co‐exposure on periodontitis prevalence.ResultsUrinary levels of 2‐aminothiazoline‐4‐carboxylic acid, mandelic acid, and N‐acetyl‐S‐(4‐hydroxy‐2‐butenyl)‐L‐cysteine were positively associated with the risk of periodontitis after adjusting for all covariates. The WQS models demonstrated a positive correlation between the mixture of VOCs and the risk of periodontitis, wherein 2‐aminothiazoline‐4‐carboxylic acid emerged as the most important contributor. The mediation analysis suggested that monocytes may play a role in the observed association between VOC co‐exposure and the prevalence of periodontitis.ConclusionsExposure to VOCs is associated with a greater prevalence of periodontitis. Monocytes' mediating role plays a crucial function in the association between the risk of periodontitis and co‐exposure to VOCs.Plain language summaryVolatile organic compounds (VOCs) are chemicals that evaporate quickly and are found all around us—from paints to cleaning products. Understanding how these compounds affect our health is crucial, especially regarding conditions like periodontitis, a common oral chronic inflammatory disease. In our study, we looked at urine samples from 1525 people who participated in a national health survey between 2011 and 2014 to find out if there is an association between VOC exposure and the risk of developing periodontitis. We found that certain chemicals in the urine, which show VOC exposure, were indeed associated with a greater risk of the disease. We further investigated the collective impact of these VOCs on the risk of periodontitis, revealing that certain chemicals exert a more significant influence than their counterparts. Additionally, our research hints at a potential role for monocytes in the interplay between VOCs and the risk of periodontitis. Our data suggest that exposure to VOCs could be associated with a greater likelihood of periodontitis, with monocytes potentia

人类与挥发性有机化合物（VOCs）之间的关系是一个长期关注的问题，因为它们的广泛来源和高蒸发速率。然而，目前有有限的直接证据表明接触挥发性有机化合物与牙周炎的发展有关。方法采用logistic回归、分位数回归和亚组分析的方法，对2011 - 2014年全国健康与营养调查（NHANES）中1525名参与者和21名尿液VOCs进行分析，探讨牙周炎风险（以附着损失（AL）和探探深度（PD）评估）与个体VOCs之间的关系。采用加权分位数和分析（WQS）和亚组分析来评估VOC混合物是否与牙周炎风险相关。使用多元线性回归模型来检验VOC共暴露与外周免疫细胞计数之间的关系。进行了一项中介分析，以评估外周免疫细胞是否参与VOC共同暴露对牙周炎患病率的影响。结果在调整所有协变量后，2 -氨基噻唑啉- 4 -羧酸、扁桃酸和N -乙酰- S -（4 -羟基- 2 -丁烯基）- L -半胱氨酸的尿水平与牙周炎的风险呈正相关。WQS模型显示，VOCs混合物与牙周炎风险呈正相关，其中2 -氨基噻唑啉- 4 -羧酸是最重要的因素。中介分析表明，单核细胞可能在观察到的VOC共暴露与牙周炎患病率之间的关联中发挥作用。结论接触挥发性有机化合物与牙周炎患病率升高有关。单核细胞的介导作用在牙周炎风险与共同暴露于voc之间的关联中起着至关重要的作用。挥发性有机化合物（VOCs）是一种快速蒸发的化学物质，在我们周围随处可见，从油漆到清洁产品。了解这些化合物如何影响我们的健康是至关重要的，尤其是在牙周炎（一种常见的口腔慢性炎症性疾病）等情况下。在我们的研究中，我们研究了参加2011年至2014年全国健康调查的1525人的尿液样本，以找出接触挥发性有机化合物与患牙周炎的风险之间是否存在关联。我们发现尿液中的某些化学物质，暴露在挥发性有机化合物中，确实与更大的患病风险有关。我们进一步调查了这些挥发性有机化合物对牙周炎风险的集体影响，揭示了某些化学物质比它们的对应物发挥更大的影响。此外，我们的研究提示了单核细胞在挥发性有机化合物和牙周炎风险之间的相互作用中的潜在作用。我们的数据表明，接触挥发性有机化合物可能与患牙周炎的可能性增加有关，单核细胞可能在这一关系中发挥作用。这项研究帮助我们更好地了解日常接触化学物质对健康的潜在影响，并强调了进一步调查环境如何影响我们健康的重要性。

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引用次数: 0

Inhibition of fibulin‐3 ameliorates periodontal inflammation through reducing M1 macrophage polarization via EGFR/PI3K/AKT pathway 抑制纤维蛋白- 3可通过EGFR/PI3K/AKT通路减少M1巨噬细胞极化，从而改善牙周炎症

IF 4.3 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-12-18 DOI: 10.1002/jper.24-0405

Hailin Mu, Beining Yang, Yan Wang, Shuo Wang, Wenqian Yu, Meie Jia, Wei Dong, Xinyi Wang, Xiaoxiao Xu, Zhipeng Dong, Baochen Yang, Xuemei Li, Jiawei Wang

BackgroundThis study aimed to evaluate the role of fibulin‐3 (FBLN3) in macrophage polarization, its mechanism, and its effect on periodontitis.MethodsWe conducted studies on periodontitis using both clinical samples and ligature‐induced mouse periodontitis model. The inflammatory state was assessed using microcomputed tomography, hematoxylin and eosin staining, immunohistochemical staining, and immunofluorescence staining. In vitro, bone marrow‐derived macrophages, and RAW 264.7 macrophages were treated with lipopolysaccharide (LPS) and interleukin (IL)‐4 to induce polarization. The role of FBLN3 in macrophage polarization was investigated using overexpression plasmids or siRNAs. Furthermore, local injection of adeno‐associated virus was employed to suppress FBLN3 expression in periodontal tissues.ResultsFBLN3 levels were greater in periodontitis tissues. FBLN3 promoted M1 polarization and suppressed M2 polarization in macrophages. The overexpression of FBLN3 promoted M1 polarization via the EGFR/PI3K/AKT signaling pathway, an effect that the epidermal growth factor receptor (EGFR) inhibitor PD153035 reversed. Suppressing FBLN3 expression improved periodontal inflammation and reduced alveolar bone loss in periodontitis.ConclusionsFBLN3 suppression can mitigate periodontitis by decreasing the M1 macrophage ratio. FBLN3 regulates M1 macrophage polarization through the EGFR/PI3K/AKT signaling pathway.Plain language summaryDisruption in the collaboration between extracellular matrix (ECM) and immune system is a significant pathology in periodontitis. Macrophages are a crucial part of the immune system and have unique functions, such as polarization. Fibulin‐3, an ECM protein, may play a vital role in this dynamic interplay. Fibulin‐3 expression is elevated in periodontitis and is closely related to immune cell function. Inhibiting fibulin‐3 can alleviate periodontitis by reducing infiltration of immune cells and M1 macrophage ratio. Furthermore, fibulin‐3 promoted macrophage M1 polarization by activating the PI3K/AKT signaling pathway through EGFR binding. Our findings offer a clinically relevant rationale for immune response modulation through fibulin‐3.

本研究旨在探讨纤维蛋白- 3 （fibulin‐3,FBLN3）在巨噬细胞极化中的作用、作用机制及其对牙周炎的影响。方法采用临床标本和结扎性小鼠牙周炎模型对牙周炎进行研究。采用微计算机断层扫描、苏木精和伊红染色、免疫组织化学染色和免疫荧光染色评估炎症状态。在体外，用脂多糖（LPS）和白细胞介素（IL）‐4处理骨髓源性巨噬细胞和RAW 264.7巨噬细胞以诱导极化。通过过表达质粒或sirna研究FBLN3在巨噬细胞极化中的作用。此外，局部注射腺相关病毒可抑制牙周组织中FBLN3的表达。结果牙周炎组织中fbln3水平较高。FBLN3促进巨噬细胞M1极化，抑制M2极化。FBLN3过表达通过EGFR/PI3K/AKT信号通路促进M1极化，这一作用被表皮生长因子受体（EGFR）抑制剂PD153035逆转。抑制FBLN3表达可改善牙周炎患者的牙周炎症，减少牙周炎患者的牙槽骨丢失。结论sfbln3抑制可通过降低M1巨噬细胞比例减轻牙周炎。FBLN3通过EGFR/PI3K/AKT信号通路调控M1巨噬细胞极化。细胞外基质（ECM）与免疫系统协同作用的破坏是牙周炎的重要病理。巨噬细胞是免疫系统的重要组成部分，具有独特的功能，如极化。Fibulin‐3，一种ECM蛋白，可能在这种动态相互作用中起重要作用。Fibulin‐3在牙周炎中表达升高，与免疫细胞功能密切相关。抑制纤维蛋白- 3可通过降低免疫细胞浸润和M1巨噬细胞比例来缓解牙周炎。此外，fibulin‐3通过EGFR结合激活PI3K/AKT信号通路，促进巨噬细胞M1极化。我们的发现为通过纤维蛋白- 3调节免疫反应提供了临床相关的理论依据。

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引用次数: 0

Effects of periodontal disease on the reproductive performance and offspring of Wistar rats. 牙周病对Wistar大鼠繁殖性能及后代的影响。

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-12-18 DOI: 10.1002/JPER.24-0281

Aline Campos Zeffa, Camila Salvador Sestario, Solange de Paula Ramos, Avacir Casanova Andrello, Andréa Name Colado Simão, Maria José Sparça Salles

Background: Periodontitis can induce systemic inflammation, and it may affect the testicles and male reproductive performance. This study investigated the effects of periodontitis on the testicles, reproductive performance, and offspring development in male rats.

Methods: Male Wistar rats were induced with periodontitis by ligating their first molars. After 14 days of inducing periodontal lesions, the animals were observed for an additional 54 days, corresponding to a complete cycle of spermatogenesis. Rats from the periodontitis group (GP, n = 12) and the control group (GC, n = 12) were paired with healthy females (n = 48) for 10 days, equivalent to 2 estrous cycles. Post-mating, the males underwent microtomographic, histological, and reproductive parameter assessments.

Results: Microtomographic analysis revealed higher porosity around the first molar in GP (26 ± 6%) and greater distance between the amelocemental junction and the alveolar bone (1.37 [1.12-1.90] mm), indicative of bone resorption. GP also exhibited significant decreases in final body weight, reduced Sertoli and Leydig cell counts, and lowered testosterone levels compared to GC. Significant morphological alterations in sperm tails were observed in GP compared to GC.

Conclusions: Periodontitis adversely affected reproductive performance, evoking, and offspring development in male rats. These findings highlight the systemic impacts of periodontal disease on male reproductive health in an animal model.

Plain language summary: Our study investigated how periodontitis can affect male reproductive health in rats and offspring development. We induced periodontitis in male rats and, after a full cycle of sperm production, these rats were mated with healthy females. We observed that the rats with periodontitis had worse reproductive performance compared to the control group without periodontitis. Additionally, the offspring of the rats with periodontitis showed signs of compromised intrauterine development and a higher incidence of congenital malformations. These results highlight that the inflammation caused by periodontitis can have adverse effects beyond the mouth, significantly impacting male reproductive health and offspring development. These findings suggest the need for further research into the clinical implications of periodontitis on reproductive health.

背景：牙周炎可引起全身性炎症，影响睾丸和男性生殖能力。本研究探讨了牙周炎对雄性大鼠睾丸、生殖性能和后代发育的影响。方法：采用结扎第一磨牙的方法诱导雄性Wistar大鼠牙周炎。在诱导牙周病变14天后，再观察动物54天，相当于一个完整的精子发生周期。将牙周炎组（GP, n = 12）和对照组（GC, n = 12）大鼠与健康雌性（n = 48）配对10天，相当于2个发情周期。交配后，雄性进行显微层析成像，组织学和生殖参数评估。结果：显微层析分析显示GP第一磨牙周围孔隙率较高（26±6%），釉骨连接处与牙槽骨之间距离较大（1.37 [1.12-1.90]mm），表明骨吸收。与GC相比，GP还表现出最终体重的显著降低，支持细胞和间质细胞计数减少，睾丸激素水平降低。与GC相比，GP组精子尾部形态发生了显著变化。结论：牙周炎对雄性大鼠的生殖性能、唤醒和后代发育有不利影响。这些发现在动物模型中强调了牙周病对男性生殖健康的系统性影响。摘要：我们的研究调查了牙周炎如何影响雄性大鼠的生殖健康和后代的发育。我们在雄性大鼠中诱导牙周炎，在精子产生完整周期后，这些大鼠与健康的雌性交配。我们观察到，与没有牙周炎的对照组相比，患有牙周炎的大鼠的生殖能力更差。此外，牙周炎大鼠的后代表现出宫内发育受损的迹象，先天性畸形的发生率更高。这些结果表明，牙周炎引起的炎症可能对口腔以外的人产生不良影响，严重影响男性生殖健康和后代的发育。这些发现表明需要进一步研究牙周炎对生殖健康的临床意义。

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引用次数: 0

Gingival crevicular fluid Bax, Bcl-xl, interleukin-22, and transforming growth factor beta 1 levels in stage III periodontitis. III 期牙周炎患者牙龈沟液中的 Bax、Bcl-xl、白细胞介素-22 和转化生长因子 beta 1 水平。

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-12-18 DOI: 10.1002/JPER.24-0356

Beral Afacan, Utkucan Budak, Ece Erdem Altınyürek, Can Özden, Özge Çevik, Timur Köse, Gülnur Emingil

Background: Intrinsic apoptosis plays a critical role in immune defense and inflammation. Its dysregulation is involved in various chronic diseases. The B-cell lymphoma 2 (Bcl-2) family primarily mediates this mitochondrial pathway. This study aimed to investigate the proapoptotic Bcl-2-associated X protein (Bax) and antiapoptotic B-cell lymphoma-extra large (Bcl-xl) levels and their association with interleukin-22 (IL-22) and transforming growth factor beta 1 (TGF-β1) in the gingival crevicular fluid (GCF) of patients with periodontitis.Methods: A total of 75 systemically healthy nonsmokers were enrolled, of whom 23 had stage III periodontitis, 26 had gingivitis, and 26 were periodontally healthy. Whole-mouth clinical periodontal measurements were recorded. Bax, Bcl-xl, IL-22, and TGF-β1 levels in the GCF were determined by enzyme-linked immunosorbent assay (ELISA). Data were analyzed using nonparametric statistical tests.Results: The periodontitis group had significantly lower GCF Bax levels than the gingivitis group (p < 0.05). The periodontitis and gingivitis groups had higher GCF Bcl-xl levels than the periodontally healthy group (p < 0.05). GCF IL-22 levels were similar in all groups (p > 0.05). The periodontitis group had lower GCF TGF-β1 levels than the gingivitis and periodontally healthy groups (p < 0.05). The diseased groups had a lower GCF Bax/Bcl-xl ratio than the healthy controls (p < 0.05). IL-22 was positively correlated with Bax (p < 0.05).Conclusions: This is the first study investigating GCF Bax and Bcl-xl levels in periodontal health and disease. Increased GCF Bcl-xl levels and a decreased Bax/Bcl-xl ratio in stage III periodontitis implicate that those apoptotic proteins may be involved in the pathogenesis of periodontal disease. Further studies are needed to enlighten the possible role of Bax and Bcl-xl and their association with IL-22 and TGF-β1 in periodontal diseases.Plain language summary: A type of cell death called intrinsic apoptosis plays an important role in the body's defense system, and its dysregulation is linked to different human diseases. The B-cell lymphoma 2-associated X protein (Bax) and B-cell lymphoma-extra large (Bcl-xl) are apoptosis-related proteins, which promote and inhibit cell death, respectively. This study aimed to investigate Bax and Bcl-xl levels and their association with the signaling proteins interleukin-22 (IL-22) and transforming growth factor beta 1 (TGF-β1) in the gingival crevicular fluid (GCF), which accumulates around the necks of the teeth of patients suffering from gum diseases such as gingivitis and periodontitis. Clinical parameters were recorded and GCF was collected. Bax, Bcl-xl, IL-22, and TGF-β1 levels were measured by biochemical assay in periodontally healthy individuals who had healthy gums (n = 26) and patients with periodontitis (n = 23) and gingivitis (n =

背景：细胞凋亡在免疫防御和炎症反应中起着至关重要的作用。它的失调与多种慢性疾病有关。b细胞淋巴瘤2 （Bcl-2）家族主要介导这种线粒体途径。本研究旨在探讨牙周炎患者龈沟液（GCF）中促凋亡bcl -2相关X蛋白（Bax）和抗凋亡b细胞淋巴瘤-特大细胞（Bcl-xl）水平及其与白细胞介素-22 （IL-22）和转化生长因子β1 （TGF-β1）的关系。方法：共纳入75名全身健康的非吸烟者，其中23人患有III期牙周炎，26人患有牙龈炎，26人牙周健康。记录全口临床牙周测量。采用酶联免疫吸附法（ELISA）检测GCF中Bax、Bcl-xl、IL-22、TGF-β1水平。数据分析采用非参数统计检验。结果：牙周炎组GCF Bax水平明显低于牙龈炎组（p < 0.05）。牙周炎组GCF TGF-β1水平低于牙龈炎组和牙周健康组(p)结论：本研究首次探讨了GCF Bax和Bcl-xl水平与牙周健康和疾病的关系。III期牙周炎GCF Bcl-xl水平升高和Bax/Bcl-xl比值降低提示这些凋亡蛋白可能参与牙周病的发病机制。Bax和Bcl-xl及其与IL-22和TGF-β1在牙周病中的可能作用有待进一步研究。一种被称为内在凋亡的细胞死亡在人体的防御系统中起着重要作用，其失调与不同的人类疾病有关。b细胞淋巴瘤2相关X蛋白（Bax）和b细胞淋巴瘤-特大（Bcl-xl）是凋亡相关蛋白，分别促进和抑制细胞死亡。本研究旨在探讨龈沟液（GCF）中Bax和Bcl-xl水平及其与白细胞介素-22 （IL-22）和转化生长因子β1 （TGF-β1）信号蛋白的关系。龈沟液是牙龈炎、牙周炎等牙龈疾病患者的牙颈周围积聚的物质。记录临床参数并收集GCF。采用生化法检测牙龈健康人群（26例）和牙周炎、牙龈炎患者（23例）的Bax、Bcl-xl、IL-22和TGF-β1水平。牙周炎患者Bax水平低于牙龈炎患者。牙周炎和牙龈炎患者的Bcl-xl水平高于健康人群，Bax/Bcl-xl比值低于健康人群。IL-22与Bax呈正相关。本研究提示凋亡调控分子可能参与牙龈疾病的发生发展，这一领域有待进一步研究。

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引用次数: 0

Ridge dimensional changes and implant stability utilizing the osseodensification protocol: A randomized clinical trial 骨脊尺寸变化和种植体稳定性利用骨密度方案：一项随机临床试验

IF 4.3 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-12-05 DOI: 10.1002/jper.24-0343

Aziz M. Saqr, Srinivas Ayilavarapu, Kavan Gandhi, Chun‐Teh Lee, Popi Stylianou

BackgroundThere is limited evidence on the effect of osseodensification drilling (OD) on alveolar ridge dimension changes and implant stability compared to standard drilling (SD). The purpose of this study was to compare the effect of both drilling protocols on ridge dimensional changes and implant stability.MethodsFifteen patients were recruited for a total of 20 pairs of implants. Pre‐ and postdrilling ridge widths were measured at the crest and 5 and 10 mm apical to the crest, using a premarked surgical guide as a reference to standardize recordings. Following implant placement, insertion torque and implant stability quotients (ISQ) were recorded. ISQ were also recorded at 3, 6, and 12 weeks post‐op. Six‐month post‐op changes in buccal bone thickness (BBT) were measured via pre‐op and post‐op cone beam computed tomography (CBCT) superimposition analysis.ResultsGreater ridge width changes were observed at the crest and 5 mm apical from the crest, favoring OD, with no difference at 10 mm. When comparing BBT 6 months post‐op, OD maintained BBT better than SD. Additionally, greater insertion torque, ISQ, and high implant stability (HIS) were observed following OD compared to SD.ConclusionOD results in greater ridge dimensional changes compared to SD following osteotomy preparation, predictably maintains BBT and reduces the risk of buccal dehiscence and resorption, obtains superior torque at placement, higher ISQ values, and a greater number of implants achieving HIS, which is maintained throughout the entire healing period.

背景：与标准钻孔（SD）相比，骨密度钻孔（OD）对牙槽嵴尺寸变化和种植体稳定性的影响证据有限。本研究的目的是比较两种钻孔方案对牙脊尺寸变化和种植体稳定性的影响。方法15例患者共20对种植体。使用预先标记的手术指南作为标准化记录的参考，在嵴处测量钻孔前和钻孔后的嵴宽度，并在顶端和顶部测量5和10毫米的嵴宽度。植入后，记录植入扭矩和种植体稳定商（ISQ）。术后3周、6周和12周分别记录ISQ。通过术前和术后锥形束计算机断层扫描（CBCT）叠加分析测量术后6个月颊骨厚度（BBT）的变化。结果牙冠和离牙冠5 mm处嵴宽度变化较大，有利于外径，10 mm处无差异。当比较术后6个月的BBT时，OD比SD更能维持BBT。此外，与SD相比，OD后观察到更大的插入扭矩，ISQ和高种植体稳定性（HIS）。结论与SD相比，od在截骨准备后导致更大的脊尺寸变化，可预测地维持BBT，降低颊裂和吸收的风险，在放置时获得更好的扭矩，更高的ISQ值，更多的种植体达到HIS，并在整个愈合期间保持。

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引用次数: 0

Salivary advanced glycated end products, their receptors, and aMMP‐8 in periodontitis patients with varying glycemic levels: A cross‐sectional study 不同血糖水平牙周炎患者唾液晚期糖基化终产物、其受体和aMMP‐8：一项横断面研究

IF 4.3 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-12-05 DOI: 10.1002/jper.24-0362

Julie Toby Thomas, Betsy Joseph, Sajit Varghese, Baiju Kamalasanan Vijayakumari, Timo Sorsa, Matti Mauramo, Sukumaran Anil, Tuomas Waltimo

BackgroundAdvanced glycation end products (AGE) and their receptors (RAGE) have been implicated in developing periodontal complications in diabetic patients. This study aimed to identify salivary AGE, RAGE, soluble RAGE (sRAGE), and active‐matrix metalloproteinase‐8 (aMMP‐8) levels at varying glycemic levels in periodontitis patients.MethodsNinety‐eight participants were categorized into uncontrolled DM‐PD group (n = 27)—periodontitis patients with uncontrolled Type 2 diabetes mellitus (T2DM) (glycated hemoglobin [HbA1c] ≥7%); controlled DM‐PD group (n = 33)—periodontitis patients with controlled T2DM (HbA1c 5.7%–6.9%); SH‐PD group (n = 18)—systemically healthy periodontitis patients; and SH‐PH group (n = 20)—systemically and periodontally healthy individuals. HbA1c along with the periodontal parameters bleeding on probing (BoP), periodontal probing depth (PPD), clinical attachment loss (CAL), number of missing teeth, and periodontal inflamed surface area (PISA) were estimated. Enzyme‐linked immunosorbent assay (ELISA) was used for analyzing salivary AGE, RAGE, sRAGE, and aMMP‐8. Multiple linear regression analysis was conducted to develop predictive models for HbA1c based on relevant predictor variables.ResultsPeriodontitis participants with uncontrolled T2DM exhibited significantly higher BoP, PPD, CAL, number of missing teeth, and PISA, along with elevated AGE, RAGE, and aMMP‐8, compared to other groups (p < 0.01). A significant positive association was observed between RAGE and HbA1c levels (p < 0.01). Among the predictors, BoP (p = 0.046) and CAL (p < 0.001) demonstrated a significant positive effect on salivary AGE. PPD was positively associated with RAGE (p < 0.05), and BoP was negatively associated with salivary sRAGE levels (p = 0.038).ConclusionsSalivary biomarkers like RAGE and aMMP‐8 exert a potential role in monitoring periodontal health and glycemic control in T2DM patients.Plain language summaryAdvanced glycation end products (AGE) and their receptors (RAGE) have been implicated in developing periodontal complications in diabetic patients. This study aimed to identify salivary AGE, RAGE, soluble RAGE (sRAGE), and aMMP‐8 levels at varying glycemic levels in periodontitis patients. Ninety‐eight participants were categorized into Group 1 (n = 27)—periodontitis patients with uncontrolled Type 2 diabetes mellitus (T2DM); Group 2 (n = 33)—periodontitis patients with controlled T2DM; Group 3 (n = 18)—systemically healthy periodontitis patients; and Group 4 (n = 20)—systemically and periodontally healthy individuals. Enzyme‐linked immunosorbent assay (ELISA

晚期糖基化终产物（AGE）及其受体（RAGE）与糖尿病患者牙周并发症的发生有关。本研究旨在确定牙周炎患者在不同血糖水平下唾液AGE、RAGE、可溶性RAGE （sRAGE）和活性基质金属蛋白酶8 （aMMP - 8）水平。方法98名受试者被分为未控制的DM - PD组(n = 27) -牙周炎合并未控制的2型糖尿病（T2DM）患者（糖化血红蛋白[HbA1c]≥7%）；对照DM - PD组(n = 33) -牙周炎患者伴控制型T2DM (HbA1c 5.7%-6.9%)；SH‐PD组(n = 18) -全身性健康牙周炎患者；和SH - PH组(n = 20) -系统和牙周健康的个体。评估HbA1c、牙周参数、牙周探诊出血（BoP）、牙周探诊深度（PPD）、临床附着损失（CAL）、缺牙数和牙周炎症表面积（PISA）。采用酶联免疫吸附法（ELISA）分析唾液AGE、RAGE、sRAGE和aMMP‐8。基于相关预测变量进行多元线性回归分析，建立HbA1c预测模型。结果与其他组相比，伴有未控制T2DM的牙周炎患者BoP、PPD、CAL、缺牙数和PISA均显著升高，AGE、RAGE和aMMP‐8均升高(p <；0.01)。RAGE与HbA1c水平呈显著正相关(p <；0.01)。预测因子中，BoP （p = 0.046）、CAL (p <；0.001)对唾液AGE有显著的积极作用。PPD与RAGE呈正相关(p <；BoP与唾液sRAGE水平呈负相关（p = 0.038）。结论唾液标志物如RAGE和aMMP‐8在监测T2DM患者牙周健康和血糖控制中发挥潜在作用。晚期糖基化终产物（AGE）及其受体（RAGE）与糖尿病患者牙周并发症的发生有关。本研究旨在确定牙周炎患者不同血糖水平下唾液AGE、RAGE、可溶性RAGE （sRAGE）和aMMP‐8水平。98名参与者被分为第1组（n = 27）——伴有2型糖尿病（T2DM）的牙周炎患者；第二组（n = 33）：牙周炎合并T2DM患者；第三组（n = 18）——全身健康的牙周炎患者；第4组(n = 20) -系统和牙周健康的个体。采用酶联免疫吸附法（ELISA）分析唾液AGE、RAGE、sRAGE和aMMP‐8。研究显示，与控制T2DM和全身健康组相比，未控制T2DM和严重牙周炎的参与者表现出明显更高的唾液AGE、RAGE和aMMP‐8水平，以及牙周参数增加。相反，未受控制的T2DM组唾液sRAGE水平明显较低。该研究还发现唾液RAGE水平与糖化血红蛋白（HbA1c）、aMMP‐8、AGE和临床牙周参数之间存在显著相关性。这项研究的结果强调了唾液生物标志物的潜在临床应用，特别是RAGE和aMMP‐8，作为评估糖尿病患者血糖控制和牙周健康的无创诊断和监测工具。

{"title":"Salivary advanced glycated end products, their receptors, and aMMP‐8 in periodontitis patients with varying glycemic levels: A cross‐sectional study","authors":"Julie Toby Thomas, Betsy Joseph, Sajit Varghese, Baiju Kamalasanan Vijayakumari, Timo Sorsa, Matti Mauramo, Sukumaran Anil, Tuomas Waltimo","doi":"10.1002/jper.24-0362","DOIUrl":"https://doi.org/10.1002/jper.24-0362","url":null,"abstract":"BackgroundAdvanced glycation end products (AGE) and their receptors (RAGE) have been implicated in developing periodontal complications in diabetic patients. This study aimed to identify salivary AGE, RAGE, soluble RAGE (sRAGE), and active‐matrix metalloproteinase‐8 (aMMP‐8) levels at varying glycemic levels in periodontitis patients.MethodsNinety‐eight participants were categorized into uncontrolled DM‐PD group (<jats:italic>n</jats:italic> = 27)—periodontitis patients with uncontrolled Type 2 diabetes mellitus (T2DM) (glycated hemoglobin [HbA1c] ≥7%); controlled DM‐PD group (<jats:italic>n</jats:italic> = 33)—periodontitis patients with controlled T2DM (HbA1c 5.7%–6.9%); SH‐PD group (<jats:italic>n</jats:italic> = 18)—systemically healthy periodontitis patients; and SH‐PH group (<jats:italic>n</jats:italic> = 20)—systemically and periodontally healthy individuals. HbA1c along with the periodontal parameters bleeding on probing (BoP), periodontal probing depth (PPD), clinical attachment loss (CAL), number of missing teeth, and periodontal inflamed surface area (PISA) were estimated. Enzyme‐linked immunosorbent assay (ELISA) was used for analyzing salivary AGE, RAGE, sRAGE, and aMMP‐8. Multiple linear regression analysis was conducted to develop predictive models for HbA1c based on relevant predictor variables.ResultsPeriodontitis participants with uncontrolled T2DM exhibited significantly higher BoP, PPD, CAL, number of missing teeth, and PISA, along with elevated AGE, RAGE, and aMMP‐8, compared to other groups (<jats:italic>p</jats:italic> < 0.01). A significant positive association was observed between RAGE and HbA1c levels (<jats:italic>p</jats:italic> < 0.01). Among the predictors, BoP (<jats:italic>p</jats:italic> = 0.046) and CAL (<jats:italic>p</jats:italic> < 0.001) demonstrated a significant positive effect on salivary AGE. PPD was positively associated with RAGE (<jats:italic>p</jats:italic> < 0.05), and BoP was negatively associated with salivary sRAGE levels (<jats:italic>p</jats:italic> = 0.038).ConclusionsSalivary biomarkers like RAGE and aMMP‐8 exert a potential role in monitoring periodontal health and glycemic control in T2DM patients.Plain language summaryAdvanced glycation end products (AGE) and their receptors (RAGE) have been implicated in developing periodontal complications in diabetic patients. This study aimed to identify salivary AGE, RAGE, soluble RAGE (sRAGE), and aMMP‐8 levels at varying glycemic levels in periodontitis patients. Ninety‐eight participants were categorized into Group 1 (<jats:italic>n</jats:italic> = 27)—periodontitis patients with uncontrolled Type 2 diabetes mellitus (T2DM); Group 2 (<jats:italic>n</jats:italic> = 33)—periodontitis patients with controlled T2DM; Group 3 (<jats:italic>n</jats:italic> = 18)—systemically healthy periodontitis patients; and Group 4 (<jats:italic>n</jats:italic> = 20)—systemically and periodontally healthy individuals. Enzyme‐linked immunosorbent assay (ELISA","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"35 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periodontitis associated with brain function impairment in middle-aged and elderly individuals with normal cognition. 牙周炎与认知能力正常的中老年人脑功能损伤有关。

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-11-20 DOI: 10.1002/JPER.24-0264

Wei Ye, Yufei Tao, Wenrui Wang, Yongqiang Yu, Xiaoshu Li

Background: The present study aimed to investigate changes in intranetwork functional connectivity (FC) and internetwork FC in middle-aged and elderly individuals with normal cognition (NC) and varying degrees of periodontitis to determine the effects of periodontitis on brain function.

Methods: Periodontal findings and resting-state functional magnetic resonance imaging data were acquired from 51 subjects with NC. Independent component analysis and correlation analysis were used for the statistical analysis of the data.

Results: Differences in intranetwork FC were observed among groups in the anterior default-mode network (aDMN), dorsal attention network and dorsal sensorimotor network (dSMN). Compared with the nonperiodontitis (NP) group or the mild-periodontitis group, the analysis of internetwork FC showed increased FC between the auditory network and the ventral attention network (VAN), between the aDMN and the salience network (SN), and between the SN and the VAN and decreased FC between the posterior default-mode network and the right frontoparietal network in the moderate-to-severe periodontitis group. Additionally, internetwork FC between the dSMN and the VAN was also increased in the moderate-to-severe periodontitis group compared to the NP group. The altered intra- and internetwork FC were significantly correlated with the periodontal clinical index.

Conclusion: Our results confirmed that periodontitis was associated with both intra- and internetwork FC changes even in NC. The present study indicates that periodontitis might be a potential risk factor for brain damage and provides a theoretical clue and a new treatment target for the early prevention of Alzheimer disease.

Plain language summary: Recent research has proposed that periodontitis is a potential risk factor for Alzheimer disease (AD). However, the relationship between periodontitis and the brain function of middle-aged and elderly individuals with normal cognition (NC) remains unclear. Analyzing the effect of periodontitis on brain function in the NC stage can provide clues to AD development and help achieve early prevention of dementia. The present study aimed to investigate changes in brain functional connectivity (FC) in NC with different severity of periodontitis to determine the effects of periodontitis on brain function. Both changed intranetwork FC and internetwork FC were found in the moderate-to-severe periodontitis group, and periodontitis was associated with brain network function impairment in NC. The present study indicates that periodontitis might be a potential risk factor for brain damage even in NC stage, and provides a theoretical clue and a new treatment target for the early prevention of AD.

研究背景本研究旨在调查具有正常认知能力（NC）和不同程度牙周炎的中老年人的网络内功能连接（FC）和网络功能连接的变化，以确定牙周炎对大脑功能的影响：方法：研究人员采集了51名NC患者的牙周检查结果和静息态功能磁共振成像数据。独立成分分析和相关分析用于数据的统计分析：结果：在前默认模式网络（aDMN）、背侧注意网络和背侧感觉运动网络（dsmN）中观察到了不同组间网络内FC的差异。与非牙周炎（NP）组或轻度牙周炎组相比，网络FC分析表明，中重度牙周炎组的听觉网络与腹侧注意网络（VAN）之间、aDMN与显著性网络（SN）之间、SN与VAN之间的网络FC增加，而后默认模式网络与右侧额顶网络之间的网络FC减少。此外，与 NP 组相比，中重度牙周炎组中 dSMN 与 VAN 之间的网络 FC 也有所增加。网络内和网络间FC的改变与牙周临床指数显著相关：我们的研究结果证实，即使在 NC 中，牙周炎也与网络内和网络 FC 的变化有关。本研究表明，牙周炎可能是脑损伤的潜在危险因素，为早期预防阿尔茨海默病提供了理论线索和新的治疗靶点：最新研究提出，牙周炎是阿尔茨海默病（AD）的潜在危险因素。然而，牙周炎与认知正常（NC）的中老年人大脑功能之间的关系仍不清楚。分析牙周炎对NC阶段脑功能的影响可以为AD的发展提供线索，有助于实现痴呆症的早期预防。本研究旨在调查不同程度牙周炎的NC患者大脑功能连接（FC）的变化，以确定牙周炎对大脑功能的影响。结果发现，中度至重度牙周炎组的脑内网络功能连接（intranetwork FC）和脑网络功能连接（internetwork FC）均发生了变化，牙周炎与数控系统的脑网络功能损伤有关。本研究表明，即使在NC阶段，牙周炎也可能是脑损伤的潜在危险因素，为早期预防AD提供了理论线索和新的治疗靶点。

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引用次数: 0

Impact of nonsurgical periodontal treatment on arterial stiffness outcomes related to endothelial dysfunction: A systematic review and meta-analysis. 非手术牙周治疗对与内皮功能障碍有关的动脉僵化结果的影响：系统回顾和荟萃分析。

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-11-16 DOI: 10.1002/JPER.24-0422

Alessandro Polizzi, Luigi Nibali, Gianluca Martino Tartaglia, Gaetano Isola

Background: To assess the available evidence on whether nonsurgical periodontal treatment (NSPT) improves arterial stiffness outcomes in patients with periodontitis (PD).Methods: Following the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines and population, intervention, comparison, outcomes, and study design (PICOS) question, electronic databases were screened for clinical interventional studies addressing the impact of NSPT on pulse wave velocity (PWV), carotid intima-media thickness (CIMT), and flow-mediated dilatation (FMD) outcomes in PD patients. Furthermore, the research strategy was implemented using a hand search. Studies were selected, and data were extracted by two independent reviewers. Random effects models were applied to perform a meta-analysis, and methodological index for nonrandomized studies (MINORS) and Cochrane Rob2 tools were used to assess the risk of bias.Results: Fifteen articles were finally included for qualitative synthesis. Among them, eight single-arm cohort studies met the final inclusion criteria for meta-analysis. The Rob2 analysis evidenced that one randomized clinical trial (RCT) had a low risk, three RCTs raised some concerns, and three RCTs had a high risk of bias, while the MINORS scores ranged from 9 to 14. The meta-analysis showed that NSPT significantly impacted FMD (p < 0.001) and CIMT (p = 0.004), while changes in PWV were not statistically significant. However, there was high heterogeneity among studies (I2 = 78% for FMD and I2 = 62% for CIMT).Conclusion: Despite some beneficial effects on FMD and CIMT, due to study limitations, high heterogeneity, and risk of bias, it cannot be concluded that NSPT is effective in improving arterial stiffness. Therefore, further studies are necessary to achieve high-quality evidence on the effect of NSPT on arterial stiffness outcomes in PD patients.Trial registration: PROSPERO ID CRD42024501399.Plain language summary: Periodontitis (PD) has been associated with alterations in arterial stiffness outcomes related to early endothelial dysfunction. Based on noninterventional studies, this meta-analysis indicates that nonsurgical periodontal treatment (NSPT) may reduce cardiovascular disease risk in patients with PD. The moderate evidence derived from the studies that were finally included showed that NSPT had beneficial effects on flow-mediated dilatation and carotid intima-media thickness, while this trend was not observed for pulse wave velocity. Moreover, the findings of the present meta-analysis were characterized by high heterogeneity and risk of bias and were derived from uncontrolled clinical trials or randomized clinical trials with limitations. Therefore, more studies with standardized protocols and homogeneous arterial stiffness outcomes are needed to elevate the qua

背景：评估非手术牙周治疗（NSPT）是否能改善牙周炎（PD）患者的动脉僵化结果：目的：评估非手术牙周治疗（NSPT）是否能改善牙周炎（PD）患者动脉僵化结果的现有证据：方法：按照系统综述和荟萃分析首选报告项目（PRISMA）指南和人群、干预、比较、结果和研究设计（PICOS）问题，在电子数据库中筛选了有关非手术牙周治疗对牙周炎患者脉搏波速度（PWV）、颈动脉内膜中层厚度（CIMT）和血流介导扩张（FMD）结果影响的临床干预研究。此外，研究策略采用人工搜索。由两名独立审稿人筛选出研究并提取数据。采用随机效应模型进行荟萃分析，并使用非随机研究方法指数（MINORS）和Cochrane Rob2工具评估偏倚风险：最终纳入 15 篇文章进行定性综合。其中，8 项单臂队列研究符合荟萃分析的最终纳入标准。Rob2 分析表明，一项随机临床试验（RCT）的偏倚风险较低，三项随机临床试验存在一些问题，三项随机临床试验的偏倚风险较高，而 MINORS 的评分在 9 到 14 分之间。荟萃分析表明，NSPT 对 FMD 有显著影响（FMD 的 p 2 = 78%，CIMT 的 I2 = 62%）：结论：尽管 NSPT 对 FMD 和 CIMT 有一些有益的影响，但由于研究的局限性、高度异质性和偏倚风险，还不能得出 NSPT 能有效改善动脉僵化的结论。因此，有必要开展进一步研究，以获得 NSPT 对帕金森病患者动脉僵化效果的高质量证据：PROSPERO ID CRD42024501399.纯文字摘要：牙周炎（PD）与早期内皮功能障碍相关的动脉僵化结果改变有关。基于非介入性研究，该荟萃分析表明非手术牙周治疗（NSPT）可降低牙周炎患者的心血管疾病风险。从最终纳入的研究中得出的中度证据显示，非手术牙周治疗对血流介导的扩张和颈动脉内膜中层厚度有有益影响，而脉搏波速度则未观察到这一趋势。此外，本荟萃分析的研究结果存在高度异质性和偏倚风险，且均来自非对照临床试验或随机临床试验，存在局限性。因此，需要进行更多具有标准化方案和同质化动脉僵化结果的研究，以提高现有证据的质量。

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引用次数: 0

Artificial intelligence with counseling on the treatment outcomes and quality of life in periodontitis patients. 人工智能对牙周炎患者治疗效果和生活质量的影响。

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-11-16 DOI: 10.1002/JPER.24-0082

Fu-Tzu You, Pei-Chen Lin, Chiung-Lin Huang, Ju-Hui Wu, Yuji Kabasawa, Chih-Chang Chen, Hsiao-Ling Huang

Background: To evaluate the effects of artificial intelligence (AI)-assisted dental monitoring (DM) with and without health counseling on the treatment outcomes and oral health-related quality of life (OHRQoL) of patients with periodontitis.

Methods: Patients with periodontitis were randomly assigned to either an AI group (AI group, n = 28), an AI and health counseling group (AIHC group, n = 27), or a control group (n = 27). All patients underwent nonsurgical periodontal treatment. Patients in the AI and AIHC groups underwent additional AI-assisted DM and AI-assisted DM with oral health counseling, respectively, for 6 months. Data on OHRQoL and periodontal measures were collected at baseline and follow-ups.

Results: At 3 months of follow-up, the AI and AIHC groups exhibited a significantly greater reduction in probing pocket depth (mean diff: -0.5 and -0.7) and clinical attachment level (mean diff: -0.5 and -0.6) compared with the control group. At 6 months of follow-up, the AI and AIHC groups exhibited a significantly greater improvement in OHRQoL (mean diff: -4.5 and -4.7) compared with the control group. At 3-month follow-up, the AIHC group exhibited a greater improvement in plaque index (mean diff: -0.2) and OHRQoL (mean diff: -4.3) compared with the AI group.

Conclusion: AI-assisted DM can be used to remind patients with periodontitis of their oral hygiene at home and effectively improve their periodontal measures and long-term OHRQoL.

Plain language summary: Gum disease is a common problem, but new technology could help. In this study, researchers looked at how AI affects gum health and quality of life (QoL). The researchers divided participants into 3 groups. One group used an AI system to monitor their gums at home. Another used AI plus got health advice. The third did not use any special technology. After 3 and 6 months, the AI groups had healthier gums, with less deep pockets and better gum attachment, compared to the group without AI. The group that also got health advice saw even greater improvements, like cleaner teeth and a bigger boost to their QoL. This is exciting because gum disease is tricky to manage alone. The AI system seems to help by reminding people to care for their teeth and gums. With expert guidance, the AI becomes an even more powerful tool for improving long-term oral health and well-being. This study shows how new technologies like AI could transform how we approach common health problems. By providing personalized support, AI can empower people to better manage their own health, leading to better outcomes.

背景：评估人工智能（AI）辅助牙科监测（DM）与健康咨询（DM）对牙周炎患者治疗效果和口腔健康相关生活质量（OHRQoL）的影响：牙周炎患者被随机分配到人工智能组（AI 组，n = 28）、人工智能和健康咨询组（AIHC 组，n = 27）或对照组（n = 27）。所有患者均接受非手术牙周治疗。AI组和AIHC组患者分别接受了为期6个月的额外AI辅助DM和AI辅助DM加口腔健康咨询。在基线和随访期间收集了有关 OHRQoL 和牙周测量的数据：随访 3 个月时，与对照组相比，人工智能组和人工口腔健康咨询组的探诊袋深度（平均差值：-0.5 和 -0.7）和临床附着水平（平均差值：-0.5 和 -0.6）明显降低。在 6 个月的随访中，与对照组相比，AI 组和 AIHC 组的 OHRQoL（平均差：-4.5 和 -4.7）显著改善。在 3 个月的随访中，与 AI 组相比，AIHC 组的斑块指数（平均差异：-0.2）和 OHRQoL（平均差异：-4.3）均有较大改善：AI辅助DM可用于提醒牙周炎患者在家注意口腔卫生，并有效改善他们的牙周措施和长期OHRQoL.Plain language summary: 牙龈疾病是一个常见问题，但新技术可以提供帮助。在这项研究中，研究人员考察了人工智能如何影响牙龈健康和生活质量（QoL）。研究人员将参与者分为 3 组。一组使用人工智能系统在家监测牙龈。另一组使用人工智能并获得健康建议。第三组没有使用任何特殊技术。3 个月和 6 个月后，与未使用人工智能的组别相比，使用人工智能的组别牙龈更健康，牙周袋更少，牙龈附着力更好。同时获得健康建议的组别改善更大，比如牙齿更整齐，生活质量也有更大提高。这令人兴奋，因为牙龈疾病很难单独控制。人工智能系统似乎可以通过提醒人们护理牙齿和牙龈来提供帮助。在专家的指导下，人工智能将成为改善长期口腔健康和福祉的更强大工具。这项研究表明，人工智能等新技术可以改变我们处理常见健康问题的方式。通过提供个性化支持，人工智能可以让人们更好地管理自己的健康，从而获得更好的结果。

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引用次数: 0

Appraising the life-course impact of Epstein-Barr virus exposure and its genetic signature on periodontitis. 评估 Epstein-Barr 病毒暴露及其基因特征对牙周炎的终生影响。

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-11-04 DOI: 10.1002/JPER.24-0300

Xinjian Ye, Jian Yuan, Yijing Bai, Yitong Chen, He Jiang, Yue Cao, Qifei Ge, Zhiyong Wang, Weiyi Pan, Shan Wang, Qianming Chen

Background: Periodontitis arises from a multifaceted interplay of environmental variables and genetic susceptibility, where microbial infection plays an indispensable part. Epstein-Barr virus (EBV) exposure has long been considered associated with periodontitis activity; however, the causal relationship and genetic connection between them remain unknown.Methods: Within a life-course context, our study employed comprehensive Mendelian randomization (MR) methods, including univariable, multivariable, Bayesian model averaging, and reverse MR, to investigate the causal association between EBV exposure and periodontitis. Additionally, linkage disequilibrium score regression and colocalization analysis were utilized to assess the cross-trait genetic correlations, followed by transcriptome-wide association and enrichment analysis to discern the genetic-phenotypic biological profiles.Results: Heightened levels of EBV antibodies, particularly early antigen diffuses (which serve as indicators of early infection or reactivation), are associated with an increased risk of periodontitis (odds ratio [OR]: 1.27 [1.09-1.47], p = 6.05 × 10-3) and demonstrate a significant genetic correlation (p = 4.11 × 10-3). This pathogenesis may involve the high-confidence causal gene RNASEK located in 17p13.1. Genetically predicted early-life anti-EBV immunoglobulin G (IgG) levels are correlated to a reduced periodontitis risk (OR: 0.89 [0.82-0.97], p = 1.76 × 10-3).Conclusions: The present study highlights the impact of life-course EBV exposure and its genetic hallmark on periodontitis, providing novel perspectives into the underlying pathogenesis and management strategies for EBV-related periodontitis. These findings underscore diverse clinical and public health implications, encompassing antiviral therapies, viral vaccination strategies, and tailored interventions for individualized periodontitis management. Further research is required to validate and expand upon our findings.Plain language summary: Periodontitis is a chronic inflammatory disease driven by interactions between microbial pathogens and the host immune system. While bacteria have traditionally been the focus of research, recent studies highlight the significance of virus-bacteria interactions, particularly the role of Epstein-Barr virus (EBV)-a herpesvirus infecting over 90% of the global population-in the development of periodontitis. However, the underlying causal and genetic mechanisms remain unclear. Our study employed genome-wide multi-omics approaches to investigate the link between EBV exposure and periodontitis. We found that recent EBV infection or reactivation increases the risk of periodontitis, whereas early-life exposure, possibly enabling immune resistance, may reduce it. Essential genes were identified as potential mediators, including CRTC3-AS1, H

背景：牙周炎产生于环境变量和遗传易感性的多方面相互作用，其中微生物感染起着不可或缺的作用。长期以来，人们一直认为EB病毒（Epstein-Barr virus，EBV）暴露与牙周炎活动有关；然而，它们之间的因果关系和遗传联系仍然未知：我们的研究采用孟德尔随机化（Mendelian randomization，MR）方法，包括单变量、多变量、贝叶斯模型平均和反向 MR，在生命过程的背景下研究 EBV 暴露与牙周炎之间的因果关系。此外，还利用连锁不平衡得分回归和共定位分析来评估跨性状遗传相关性，然后利用全转录组关联分析和富集分析来鉴别遗传表型生物特征：结果：EB病毒抗体水平升高，尤其是早期抗原扩散（作为早期感染或再激活的指标），与牙周炎风险增加有关（比值比[OR]：1.27 [1.09-1.47]，p = 6.05 × 10-3），并显示出显著的遗传相关性（p = 4.11 × 10-3）。这种发病机制可能涉及位于 17p13.1 的高置信度致病基因 RNASEK。遗传预测的生命早期抗 EBV 免疫球蛋白 G (IgG) 水平与牙周炎风险降低相关（OR：0.89 [0.82-0.97]，p = 1.76 × 10-3）：本研究强调了EBV暴露及其遗传特征对牙周炎的影响，为EBV相关牙周炎的潜在发病机制和管理策略提供了新的视角。这些发现强调了不同的临床和公共卫生影响，包括抗病毒疗法、病毒疫苗接种策略和个性化牙周炎管理的定制干预措施。我们还需要进一步的研究来验证和扩展我们的发现。白话摘要：牙周炎是一种慢性炎症性疾病，由微生物病原体和宿主免疫系统之间的相互作用驱动。虽然细菌一直是研究的重点，但最近的研究强调了病毒-细菌相互作用的重要性，尤其是 Epstein-Barr 病毒（EBV）--一种感染全球 90% 以上人口的疱疹病毒--在牙周炎发病中的作用。然而，潜在的成因和遗传机制仍不清楚。我们的研究采用了全基因组多组学方法来研究 EBV 暴露与牙周炎之间的联系。我们发现，近期的 EBV 感染或再激活会增加牙周炎的风险，而早年的暴露则可能会降低牙周炎的风险，因为早年的暴露可能会增强免疫抵抗力。基本基因被确定为潜在的介导因素，包括 CRTC3-AS1、HLA-DQA1 和 RNASEK。这些研究结果为了解 EBV 与牙周炎的关系提供了新的视角。例如，病毒检测和控制可使对标准细菌疗法无反应的患者受益，通过接种疫苗及早接触病毒可降低牙周炎的风险。要阐明这些基本机制以及病毒与细菌相互作用的贡献，还需要进一步的临床研究。

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