Journal of periodontology最新文献_第7页

Survival rate of teeth adjacent and nonadjacent to dental implants: A retrospective cohort study 牙科种植体相邻和非相邻牙齿的存活率：回顾性队列研究

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-05-14 DOI: 10.1002/JPER.23-0739

Hsuan-Hung Chen, Guo-Hao Lin, Richard T. Kao, Yu-Ting Yeh

Background

The aim of this retrospective study was to investigate the risk of tooth loss for teeth adjacent and nonadjacent to dental implants.

Methods

A total of 787 patients with an average follow-up of 57.1 months were examined to define the tooth loss, cumulative survival rate, and odds ratio (OR) for teeth adjacent versus nonadjacent to implants. A multivariate logistic regression was employed to assess the association between dental history and various recorded etiologies of tooth loss among teeth adjacent to implants.

Results

The incidence of tooth loss for teeth adjacent to implants was 8.1% at the tooth level and 15.1% at the patient level, while 0.7% and 9.5% at the tooth and patientlevel for teeth nonadjacent to implants. The 10-year cumulative survival rate for teeth adjacent to implants was 89.2%, and the primary etiology of tooth loss was root fracture (45.2%). The risk of tooth loss among teeth adjacent versus nonadjacent to implants was significantly higher (OR 13.15). Among teeth adjacent to implants, root canal-treated teeth had a significantly higher risk of tooth loss due to root fracture (OR 7.72), a history of existing restoration significantly increased the risk of tooth loss due to caries (OR 3.05), and a history of periodontitis significantly increased the risk of tooth loss due to periodontitis (OR 38.24).

Conclusion

The present study demonstrated that after patients received dental implant treatment, teeth adjacent to implants showed a 13.2-fold higher risk of tooth loss compared to teeth nonadjacent to implants, with the primary etiology being root fracture.

背景：这项回顾性研究的目的是调查牙齿种植体相邻和非相邻牙齿脱落的风险：这项回顾性研究的目的是调查与种植体相邻和非相邻的牙齿脱落的风险：对平均随访 57.1 个月的 787 名患者进行了研究，以确定与种植体相邻和非相邻牙齿的脱落率、累积存活率和几率比 (OR)。采用多变量逻辑回归评估牙科病史与种植体邻近牙齿脱落的各种病因之间的关联：结果：与种植体相邻的牙齿脱落发生率在牙齿层面为 8.1%，在患者层面为 15.1%，而与种植体不相邻的牙齿脱落发生率在牙齿和患者层面分别为 0.7% 和 9.5%。种植体邻近牙齿的 10 年累积存活率为 89.2%，牙齿脱落的主要病因是牙根折断（45.2%）。与种植体相邻的牙齿与非相邻的牙齿相比，牙齿脱落的风险明显更高（OR 13.15）。在与种植体相邻的牙齿中，根管治疗过的牙齿因牙根折断导致牙齿脱落的风险明显更高（OR 7.72），有过修复史的牙齿因龋齿导致牙齿脱落的风险明显增加（OR 3.05），有过牙周炎的牙齿因牙周炎导致牙齿脱落的风险明显增加（OR 38.24）：本研究表明，患者接受种植牙治疗后，种植体邻近牙齿的脱落风险是非种植体邻近牙齿的13.2倍，主要病因是牙根折断。

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引用次数: 0

Peri-implant diseases and systemic inflammation: A preliminary analysis from a cross-sectional survey of patients with hypertension 种植体周围疾病与全身炎症：对高血压患者横断面调查的初步分析。

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-05-14 DOI: 10.1002/JPER.21-0338

Marco Orlandi, Nicola Pranno, Vipul Patel, Piero Papi, Bianca Di Murro, Giorgio Pompa, Antonella Polimeni, Claudio Letizia, Jeanie Suvan, Francesco D'Aiuto

Background

The aim of this study was to investigate the association between peri-implant diseases and systemic inflammation assessed by serum C-reactive protein (CRP) levels in a sample of patients with hypertension.

Methods

A total of 151 participants with hypertension were included in a cross-sectional study. The population was divided into six groups according to their peri-implant and periodontal status (healthy controls, mucositis, peri-implantitis, periodontitis, periodontitis and mucositis, periodontitis, and peri-implantitis). Linear, logistic regression, and correlation analyses were performed.

Results

CRP levels were statistically significantly higher in participants with periodontitis alone (median 3.2 mg/L, interquartile range [IQR] 1.8, p = 0.012), combined with mucositis (3.10 mg/L, IQR 2.35, p < 0.001) or peri-implantitis (2.7 mg/L, IQR 2.53, p = 0.002) when compared to the healthy controls (1 mg/L, IQR 1.2). This association was independent of age, sex, smoking status, and adiposity differences. Participants with periodontitis with and without peri-implant diseases had the greatest odds of exhibiting CRP > 3 mg/L (odds ratio = 7.3, 95% confidence interval 1.6–33.9).

Conclusions

Peri-implant diseases are associated with systemic inflammation, but the nature of the association should be further investigated.

背景：本研究旨在调查高血压患者血清C反应蛋白（CRP）水平评估的种植体周围疾病与全身炎症之间的关系：本研究旨在调查高血压患者样本中种植体周围疾病与通过血清C反应蛋白（CRP）水平评估的全身炎症之间的关系：一项横断面研究共纳入了 151 名高血压患者。根据种植体周围和牙周状况（健康对照组、粘膜炎组、种植体周围炎组、牙周炎组、牙周炎和粘膜炎组、牙周炎组和种植体周围炎组）将人群分为六组。进行了线性、逻辑回归和相关分析：仅患有牙周炎（中位数 3.2 mg/L，四分位数间距 [IQR]1.8，P = 0.012）、合并粘膜炎（3.10 mg/L，IQR 2.35，P 3 mg/L，几率比例 = 7.3，95% 置信区间 1.6-33.9）的参与者 CRP 水平明显更高：种植体周围疾病与全身炎症有关，但这种关联的性质有待进一步研究。

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引用次数: 0

Associations between periodontitis and serum anti-malondialdehyde–acetaldehyde antibody concentrations in rheumatoid arthritis: A case-control study 类风湿性关节炎患者牙周炎与血清抗丙二醛-乙醛抗体浓度之间的关系：病例对照研究。

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-05-10 DOI: 10.1002/JPER.23-0604

Joyce A. Lee, Ted R. Mikuls, Harlan R. Sayles, Geoffrey M. Thiele, Michael J. Duryee, Jeffrey B. Payne

Background

Malondialdehyde–acetaldehyde (MAA) adducts lead to generation of anti-MAA autoantibodies and have been independently identified in inflamed periodontal and rheumatoid arthritis (RA) tissues. This study evaluates serum samples from RA cases and osteoarthritis (OA) controls to quantify associations between periodontal clinical measures, alveolar bone loss (ABL), and anti-Porphyromonas gingivalis, anti-Prevotella intermedia, and anti-Fusobacterium nucleatum antibody concentrations with anti-MAA antibody concentrations.

Methods

Participants (n = 284 RA cases, n = 330 OA controls) underwent periodontal clinical assessments and ABL measurements. Serum immunoglobulin (Ig) A, IgG, and IgM anti-MAA and serum IgG antibacterial antibody concentrations were quantified by enzyme-linked immunosorbent assay (ELISA). Analyses utilized simple linear regression and multivariable adjusted models.

Results

No significant associations of periodontal clinical measures with serum anti-MAA were found. Moderate (p = 0.038 and p = 0.036, respectively) and high ABL (p = 0.012 and p = 0.014, respectively) in RA cases (but not in OA) were positively associated with IgG and IgM anti-MAA. Anti-P. gingivalis and anti-P. intermedia antibody concentrations were positively associated with IgA (p = 0.001 for both), IgG (p = 0.007 and p = 0.034, respectively), and IgM anti-MAA antibody concentrations (p < 0.001 and p = 0.020, respectively), while anti-F. nucleatum was positively associated with IgG anti-MAA (p = 0.042), findings that were similar across groups.

Conclusions

A positive association was demonstrated between ABL and serum IgG and IgM anti-MAA antibody concentrations that was unique to RA and not observed in OA. Serum anti-P. gingivalis, anti-P. intermedia, and anti-F. nucleatum antibody concentrations displayed significant associations with anti-MAA antibody in both groups. These findings suggest MAA may play a role in the interrelationship between the periodontium and RA.

背景：丙二醛-乙醛（MAA）加合物会导致产生抗MAA自身抗体，并已在发炎的牙周和类风湿性关节炎（RA）组织中独立发现。本研究评估了 RA 病例和骨关节炎（OA）对照组的血清样本，以量化牙周临床指标、牙槽骨损失（ABL）、抗牙龈卟啉单胞菌、抗中间前伏菌和抗核分枝杆菌抗体浓度与抗 MAA 抗体浓度之间的关联：参与者（n = 284 例 RA 病例，n = 330 例 OA 对照组）接受牙周临床评估和 ABL 测量。血清免疫球蛋白 (Ig) A、IgG 和 IgM 抗MAA 以及血清 IgG 抗菌抗体浓度通过酶联免疫吸附试验 (ELISA) 进行量化。分析采用简单线性回归和多变量调整模型：结果：未发现牙周临床指标与血清抗 MAA 有明显关联。RA病例中的中度（p = 0.038，分别为0.038和0.036）和高度ABL（p = 0.012和0.014，分别为0.012和0.014）与IgG和IgM抗MAA呈正相关。抗牙龈脓肿和抗中间型牙龈脓肿抗体浓度与 IgA（两者均为 p = 0.001）、IgG（分别为 p = 0.007 和 p = 0.034）和 IgM 抗MAA 抗体浓度呈正相关（p 结论）：ABL 与血清 IgG 和 IgM 抗MAA 抗体浓度之间呈正相关，这是 RA 所特有的，在 OA 中未观察到。两组患者的血清抗牙龈脓肿、抗中间脓肿和抗核脓肿抗体浓度均与抗 MAA 抗体有显著关联。这些研究结果表明，MAA可能在牙周和RA之间的相互关系中发挥作用。

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引用次数: 0

Angiogenic potential in periodontal stem cells from upper and lower jaw: A pilot study 上下颌骨牙周干细胞的血管生成潜力：试点研究

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-05-06 DOI: 10.1002/JPER.24-0070

Hanna Malyaran, Chloé Radermacher, Rogerio B. Craveiro, Mark P. Kühnel, Danny Jonigk, Michael Wolf, Sabine Neuss

Background

Teeth and supporting oral tissues are attractive and accessible sources of stem cells. Periodontal ligament stem cells (PDLSC) are readily isolated from extracted third molars, and exhibit the ability to self-renew and differentiate into multiple mesodermal cell fates. Clinical experience suggests that the exact location of periodontal defects affects the oral bone remodeling and wound healing. Compared to the mandible, the maxilla heals quicker and more efficiently. Angiogenesis is key in tissue regeneration including dental tissues, yet few studies focus on the angiogenic potential of PDLSC, none of which considered the differences between upper and lower jaw PDLSC (u-PDLSC and l-PDLSC, respectively).

Methods

Here we studied the angiogenic potential of u-PDLSC and l-PDLSC and compared the results to well-established mesenchymal stem cells (MSC). Cells were characterized in terms of surface markers, proliferation, and vascular endothelial growth factor (VEGF) secretion, and angiogenic assays were performed. Newly formed capillaries were stained with CD31, and their expression of platelet endothelial cell adhesion molecule (PECAM-1), angiopoietin 2 (ANGPT2), and vascular endothelial growth factor receptor 1 and 2 (VEGFR-1, VEGFR-2) were measured.

Results

Periodontal stem cells from the upper jaw showed a higher proliferation capacity, secreted more VEGF, and formed capillary networks faster and denser than l-PDLSC. Gene expression of angiogenesis-related genes was significantly higher in u-PDLSC than in l-PDLSC or MSC, given that culture conditions were suitable.

Conclusion

The oral cavity is a valuable source of stem cells, particularly PDLSC, which are promising for oral tissue engineering due to their robust growth, lifelong accessibility, low immunogenicity, and strong differentiation potential. Notably, u-PDLSC exhibit higher VEGF secretion and accelerate capillary formation compared to l-PDLSC or MSC. This study suggests a potential molecular mechanism in capillary formation, emphasizing the significance of precise location isolation of PDLSC.

背景牙齿和支持性口腔组织是极具吸引力且容易获取的干细胞来源。牙周韧带干细胞（PDLSC）很容易从拔出的第三磨牙中分离出来，并表现出自我更新和分化为多种中胚层细胞命运的能力。临床经验表明，牙周缺损的确切位置会影响口腔骨重塑和伤口愈合。与下颌骨相比，上颌骨的愈合速度更快，效率更高。血管生成是包括牙科组织在内的组织再生的关键，但很少有研究关注PDLSC的血管生成潜能，也没有研究考虑到上颌和下颌PDLSC（分别为u-PDLSC和l-PDLSC）之间的差异。我们对细胞的表面标记、增殖和血管内皮生长因子（VEGF）分泌进行了表征，并进行了血管生成试验。用CD31对新形成的毛细血管进行染色，并测量其血小板内皮细胞粘附分子（PECAM-1）、血管生成素2（ANGPT2）、血管内皮生长因子受体1和2（VEGFR-1、VEGFR-2）的表达。在培养条件适宜的情况下，u-PDLSC血管生成相关基因的表达明显高于l-PDLSC或间叶干细胞。值得注意的是，与l-PDLSC或间叶干细胞相比，u-PDLSC表现出更高的血管内皮生长因子分泌量，并能加速毛细血管的形成。这项研究提出了毛细血管形成的潜在分子机制，强调了精确定位分离 PDLSC 的重要性。

{"title":"Angiogenic potential in periodontal stem cells from upper and lower jaw: A pilot study","authors":"Hanna Malyaran, Chloé Radermacher, Rogerio B. Craveiro, Mark P. Kühnel, Danny Jonigk, Michael Wolf, Sabine Neuss","doi":"10.1002/JPER.24-0070","DOIUrl":"10.1002/JPER.24-0070","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Teeth and supporting oral tissues are attractive and accessible sources of stem cells. Periodontal ligament stem cells (PDLSC) are readily isolated from extracted third molars, and exhibit the ability to self-renew and differentiate into multiple mesodermal cell fates. Clinical experience suggests that the exact location of periodontal defects affects the oral bone remodeling and wound healing. Compared to the mandible, the maxilla heals quicker and more efficiently. Angiogenesis is key in tissue regeneration including dental tissues, yet few studies focus on the angiogenic potential of PDLSC, none of which considered the differences between upper and lower jaw PDLSC (u-PDLSC and l-PDLSC, respectively).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Here we studied the angiogenic potential of u-PDLSC and l-PDLSC and compared the results to well-established mesenchymal stem cells (MSC). Cells were characterized in terms of surface markers, proliferation, and vascular endothelial growth factor (VEGF) secretion, and angiogenic assays were performed. Newly formed capillaries were stained with CD31, and their expression of platelet endothelial cell adhesion molecule (PECAM-1), angiopoietin 2 (ANGPT2), and vascular endothelial growth factor receptor 1 and 2 (VEGFR-1, VEGFR-2) were measured.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Periodontal stem cells from the upper jaw showed a higher proliferation capacity, secreted more VEGF, and formed capillary networks faster and denser than l-PDLSC. Gene expression of angiogenesis-related genes was significantly higher in u-PDLSC than in l-PDLSC or MSC, given that culture conditions were suitable.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The oral cavity is a valuable source of stem cells, particularly PDLSC, which are promising for oral tissue engineering due to their robust growth, lifelong accessibility, low immunogenicity, and strong differentiation potential. Notably, u-PDLSC exhibit higher VEGF secretion and accelerate capillary formation compared to l-PDLSC or MSC. This study suggests a potential molecular mechanism in capillary formation, emphasizing the significance of precise location isolation of PDLSC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"95 7","pages":"662-672"},"PeriodicalIF":4.2,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/JPER.24-0070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140845953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inflammation and tissue remodeling mediator expression during gingivitis: A comparison between experimental, naturally occurring gingivitis, and periodontal health 牙龈炎期间炎症和组织重塑介质的表达：实验性牙龈炎、自然牙龈炎与牙周健康之间的比较

IF 4.3 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-05-06 DOI: 10.1002/jper.23-0692

Yung‐Ting Hsu, Ana M. Chang, Diane Daubert, Frank Roberts, Dandan Chen, Harsh M. Trivedi, Juliana Gomez, Rich P. Darveau

BackgroundThe aim of this study is to evaluate the immune regulation and tissue remodeling responses during experimental gingivitis (EG) and naturally occurring gingivitis (NG) to provide a comprehensive analysis of host responses. Gingival crevicular fluid (GCF) was obtained from 2 human studies conducted in university settings.MethodsThe EG study enrolling 26 volunteers provided controls for the baseline (Day 0) from healthy disease‐free participants, while Day 21 (the end of EG induction of the same group) was used to represent EG. Twenty‐six NG participants age‐matched with those of the EG group were recruited. GCF samples were analyzed for 39 mediators of inflammatory/immune responses and tissue remodeling using commercially available bead‐based multiplex immunoassays. The differences in GI and mediator expression among groups were determined at a 95% confidence level (p ≤ 0.05) by a 2‐way analysis of variance (ANOVA) with a post‐hoc Tukey's test.ResultsOur findings showed that EG had a greater gingival index than NG and was healthy (p < 0.01 of all comparisons). Furthermore, EG showed significantly higher levels of MPO (p < 0.001), CCL3 (p < 0.05), and IL‐1B (p < 0.001) than NG. In contrast, NG had increased levels of MIF (p < 0.05), Fractalkine (p < 0.001), angiogenin (p < 0.05), C3a (p < 0.001), BMP‐2 (p < 0.001), OPN (p < 0.05), RANKL (p < 0.001), and MMP‐13 (p < 0.001) than EG.ConclusionsConsistent with the findings from chronic (NG) versus acute (EG) inflammatory lesions, these data reveal that NG displays greater immune regulation, angiogenesis, and bone remodeling compared to EG.

背景本研究旨在评估实验性牙龈炎（EG）和自然发生的牙龈炎（NG）期间的免疫调节和组织重塑反应，从而对宿主反应进行全面分析。方法EG研究为26名志愿者提供了基线（第0天）对照，来自健康无病的参与者，而第21天（同组参与者EG诱导结束时）则代表EG。另外还招募了 26 名与 EG 组年龄相匹配的 NG 参与者。使用市售的基于微珠的多重免疫测定法对 GCF 样品中的 39 种炎症/免疫反应和组织重塑介质进行了分析。结果我们的研究结果表明，与 NG 相比，EG 的牙龈指数更高，而且更健康（所有比较的 p 均为 0.01）。此外，EG 的 MPO（p < 0.001）、CCL3（p < 0.05）和 IL-1B （p < 0.001）水平明显高于 NG。相比之下，NG 的 MIF（p <；0.05）、Fractalkine（p <；0.001）、血管生成素（p <；0.05）、C3a（p <；0.001）、BMP-2（p <；0.001）、OPN（p <；0.05）、RANKL（p <；0.001）和 MMP-13 （p <；0.结论与慢性（NG）与急性（EG）炎性病变的研究结果一致，这些数据显示，与 EG 相比，NG 显示出更强的免疫调节、血管生成和骨重塑能力。

{"title":"Inflammation and tissue remodeling mediator expression during gingivitis: A comparison between experimental, naturally occurring gingivitis, and periodontal health","authors":"Yung‐Ting Hsu, Ana M. Chang, Diane Daubert, Frank Roberts, Dandan Chen, Harsh M. Trivedi, Juliana Gomez, Rich P. Darveau","doi":"10.1002/jper.23-0692","DOIUrl":"https://doi.org/10.1002/jper.23-0692","url":null,"abstract":"BackgroundThe aim of this study is to evaluate the immune regulation and tissue remodeling responses during experimental gingivitis (EG) and naturally occurring gingivitis (NG) to provide a comprehensive analysis of host responses. Gingival crevicular fluid (GCF) was obtained from 2 human studies conducted in university settings.MethodsThe EG study enrolling 26 volunteers provided controls for the baseline (Day 0) from healthy disease‐free participants, while Day 21 (the end of EG induction of the same group) was used to represent EG. Twenty‐six NG participants age‐matched with those of the EG group were recruited. GCF samples were analyzed for 39 mediators of inflammatory/immune responses and tissue remodeling using commercially available bead‐based multiplex immunoassays. The differences in GI and mediator expression among groups were determined at a 95% confidence level (p ≤ 0.05) by a 2‐way analysis of variance (ANOVA) with a post‐hoc Tukey's test.ResultsOur findings showed that EG had a greater gingival index than NG and was healthy (p < 0.01 of all comparisons). Furthermore, EG showed significantly higher levels of MPO (p < 0.001), CCL3 (p < 0.05), and IL‐1B (p < 0.001) than NG. In contrast, NG had increased levels of MIF (p < 0.05), Fractalkine (p < 0.001), angiogenin (p < 0.05), C3a (p < 0.001), BMP‐2 (p < 0.001), OPN (p < 0.05), RANKL (p < 0.001), and MMP‐13 (p < 0.001) than EG.ConclusionsConsistent with the findings from chronic (NG) versus acute (EG) inflammatory lesions, these data reveal that NG displays greater immune regulation, angiogenesis, and bone remodeling compared to EG.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":"51 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140845543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of minimally invasive non-surgical therapy on C-reactive protein, lipoprotein-associated phospholipase A2, and clinical outcomes in periodontitis patients: A 1-year randomized, controlled clinical trial 微创非手术疗法对牙周炎患者 C 反应蛋白、脂蛋白相关磷脂酶 A2 和临床疗效的影响：为期一年的随机对照临床试验

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-05-02 DOI: 10.1002/JPER.23-0518

Gaetano Isola, Paolo Pesce, Alessandro Polizzi, Antonino Lo Giudice, Marco Cicciù, Frank A. Scannapieco

Background

Growing evidence suggests the type of periodontal treatment could differentially influence the reduction of key cardiovascular risk mediators in periodontitis patients. This randomized, controlled clinical trial compared the impact of minimally invasive non-surgical therapy (MINST) with quadrant-wise subgingival instrumentation (Q-SI) on C-reactive protein (CRP) together with lipoprotein-associated phospholipase A₂ (Lp-PLA₂) levels, and clinical periodontal outcomes in patients with periodontitis. Moreover, it was evaluated if baseline CRP levels impacted the efficacy of non-surgical periodontal therapy protocols.

Methods

Forty-two periodontitis patients were enrolled and randomly treated by means of MINST (n = 21) or Q-SI (n = 21). The outcomes assessed were serum CRP and Lp-PLA₂, and periodontal parameters (probing depth [PD], clinical attachment level [CAL], full-mouth bleeding score [FMBS]), at baseline and at follow-ups at 1, 3, and 6 months and at 1 year after treatment.

Results

At 1 year, MINST significantly reduced, among others, mean PD (p = 0.007), mean CAL (p = 0.007), the number of pockets >4 mm (p = 0.011) and ≥6 mm (p = 0.005), and FMBS (p = 0.048) compared to Q-SI. Generalized multivariate analysis evidenced that high baseline CRP (p = 0.039) and FMBS (p = 0.046) levels, together with MINST treatment (p = 0.007) were significant predictors of PD reduction at 1-year follow-up. Moreover, the Jonckheere–Terpstra test showed that patients with high baseline CRP levels gained more benefits from MINST treatment at 1-year follow-up than they did from Q-SI.

Conclusion

Patients receiving MINST showed a greater reduction in CRP levels than patients with Q-SI after 1 year of follow-up. Moreover, patients with high baseline levels of CRP and Lp-PLA₂ gained more benefits from the MINST approach at 1-year follow-up.

背景越来越多的证据表明，牙周治疗的类型会对牙周炎患者主要心血管风险介质的降低产生不同程度的影响。这项随机对照临床试验比较了微创非手术疗法（MINST）和四维龈下器械治疗（Q-SI）对牙周炎患者C反应蛋白（CRP）、脂蛋白相关磷脂酶A2（Lp-PLA2）水平和临床牙周疗效的影响。此外，该研究还评估了基线 CRP 水平是否会影响非手术牙周治疗方案的疗效。评估的结果包括基线时、治疗后 1、3、6 个月和 1 年的随访时的血清 CRP 和 Lp-PLA2 以及牙周参数（探诊深度 [PD]、临床附着水平 [CAL]、全口出血评分 [FMBS]）。结果与 Q-SI 相比，MINST 在治疗 1 年后显著降低了平均 PD（p = 0.007）、平均 CAL（p = 0.007）、4 mm（p = 0.011）和≥6 mm（p = 0.005）牙槽数以及 FMBS（p = 0.048）等指标。广义多变量分析表明，高基线 CRP（p = 0.039）和 FMBS（p = 0.046）水平以及 MINST 治疗（p = 0.007）是随访 1 年时 PD 下降的重要预测因素。此外，Jonckheere-Terpstra 检验表明，基线 CRP 水平高的患者在 1 年随访时从 MINST 治疗中获得的益处比从 Q-SI 治疗中获得的益处更大。此外，CRP 和 Lp-PLA2 基线水平较高的患者在 1 年的随访中从 MINST 方法中获益更多。

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引用次数: 0

Salivary microbiome and MRP‐8/14 levels in children with gingivitis, healthy children, and their mothers 牙龈炎儿童、健康儿童及其母亲的唾液微生物群和 MRP-8/14 水平

IF 4.3 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-05-02 DOI: 10.1002/jper.23-0632

Alice Blufstein, Natasa Pejcic, Kathrin Spettel, Bela Hausmann, David Seki, Tugba Ertekin, Julia Hinrichs‐Priller, Sarra Altner, Marion Nehr, Katrin Bekes, Athanasios Makristathis, Oleh Andrukhov

BackgroundGingivitis is the most common form of periodontal disease among children and adolescents and is associated with disrupted host–microbiome homeostasis. Family is an important factor influencing the prevalence of gingivitis. In the present study, we investigated the salivary microbiome, oral hygiene habits, and the salivary level of myeloid‐related protein (MRP)‐8/14 in children aged 7–12 years with gingivitis, periodontally healthy children, and their mothers.MethodsThis study included 24 children with gingivitis (including four sibling pairs) and 22 periodontally healthy children (including two sibling pairs) and their mothers. The whole saliva was collected, DNA was extracted, the variable V3–V4 region of the eubacterial 16S ribosomal RNA gene was amplified, and sample library preparation was performed according to the Illumina protocol. The salivary levels of MRP‐8/14 were analyzed by ELISA.ResultsAlpha diversity of the salivary microbiome was considerably higher in gingivitis children and mothers of gingivitis children compared to healthy children and their mothers, respectively. Significant differences in beta diversity between healthy and gingivitis children, healthy children and their mothers, and gingivitis children and their mothers were detected. Overall, the number of common core amplicon sequence variants between children and their own mothers was significantly higher than between children and other mothers. The salivary MRP‐8/14 levels in children with gingivitis were significantly higher compared to healthy children; a similar tendency was also mentioned for mothers.ConclusionOur study underlines the importance of family as an essential factor influencing oral health.

背景牙龈炎是儿童和青少年中最常见的牙周疾病，与宿主-微生物组平衡紊乱有关。家庭是影响牙龈炎发病率的一个重要因素。本研究调查了 7-12 岁牙龈炎儿童、牙周健康儿童及其母亲的唾液微生物组、口腔卫生习惯和唾液中髓样相关蛋白（MRP）-8/14 的水平。采集全唾液，提取DNA，扩增真细菌16S核糖体RNA基因的可变V3-V4区，并按照Illumina协议进行样本库制备。结果与健康儿童及其母亲相比，牙龈炎儿童及其母亲的唾液微生物组的α多样性要高得多。健康儿童和牙龈炎儿童、健康儿童及其母亲以及牙龈炎儿童及其母亲之间的β多样性存在显著差异。总体而言，儿童与其母亲之间的共同核心扩增片段序列变异数明显高于儿童与其他母亲之间的共同核心扩增片段序列变异数。与健康儿童相比，牙龈炎儿童唾液中的 MRP-8/14 水平明显更高；母亲也有类似的趋势。

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引用次数: 0

Effects of intermittent fasting on periodontal inflammation and subgingival microbiota 间歇性禁食对牙周炎症和龈下微生物群的影响

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-04-24 DOI: 10.1002/JPER.23-0676

Ronaldo Lira-Junior, Micheál Mac Aogáin, Eva Crncalo, Neda Rajamand Ekberg, Sanjay H. Chotirmall, Sven Pettersson, Anders Gustafsson, Kerstin Brismar, Nagihan Bostanci

Background

Studies on the impact of intermittent fasting on periodontal health are still scarce. Thus, this study evaluated the effects of long-term intermittent fasting on periodontal health and the subgingival microbiota.

Methods

This pilot study was part of a nonrandomized controlled trial. Overweight/obese participants (n = 14) entered an intermittent fasting program, specifically the 5:2 diet, in which they restricted caloric intake to about a quarter of the normal total daily caloric expenditure for two nonconsecutive days/week. Subjects underwent a thorough clinical and laboratory examination, including an assessment of their periodontal condition, at baseline and 6 months after starting the diet. Additionally, subgingival microbiota was assessed by 16S rRNA gene sequencing.

Results

After 6 months of intermittent fasting, weight, body mass index, C-reactive protein, hemoglobin A1c (HbA1c), and the cholesterol profile improved significantly (p < 0.05). Moreover, significant reductions were observed in bleeding on probing (p = 0.01) and the presence of shallow periodontal pockets after fasting (p < 0.001), while no significant change was seen in plaque index (p = 0.14). While we did not observe significant changes in α- or β-diversity of the subgingival microbiota related to dietary intervention (p > 0.05), significant differences were seen in the abundances of several taxa among individuals exhibiting ≥60% reduction (good responders) in probing pocket depth of 4–5 mm compared to those with <60% reduction (bad responders).

Conclusion

Intermittent fasting decreased systemic and periodontal inflammation. Although the subgingival microbiota was unaltered by this intervention, apparent taxonomic variability was observed between good and bad responders.

背景有关间歇性禁食对牙周健康影响的研究仍然很少。因此，本研究评估了长期间歇性禁食对牙周健康和龈下微生物群的影响。方法本试验研究是非随机对照试验的一部分。超重/肥胖参与者（n = 14）参加了一项间歇性禁食计划，特别是 5:2 饮食计划，在该计划中，他们将热量摄入限制在正常日总热量消耗的四分之一左右，连续两天/周。受试者在基线期和开始节食 6 个月后接受了全面的临床和实验室检查，包括牙周状况评估。结果间歇性禁食 6 个月后，受试者的体重、体重指数、C 反应蛋白、血红蛋白 A1c (HbA1c) 和胆固醇含量均有显著改善（p < 0.05）。此外，在禁食后，探诊出血（p = 0.01）和牙周浅袋（p < 0.001）明显减少，而牙菌斑指数没有明显变化（p = 0.14）。虽然我们没有观察到与饮食干预有关的龈下微生物群的α或β多样性的显著变化（p >0.05），但与探查袋深度减少 60% 的个体（良好响应者）相比，探查袋深度减少 4-5 mm 的个体（不良响应者）在几个类群的丰度上存在显著差异。虽然龈下微生物群未因这一干预措施而发生改变，但在良好反应者和不良反应者之间观察到了明显的分类差异。

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引用次数: 0

Patient awareness of the association between periodontal and systemic diseases in an academic setting 学术机构中患者对牙周病与全身性疾病之间关联的认识

IF 4.3 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-04-11 DOI: 10.1002/jper.23-0635

William Carter, Tamanna Tiwari, Satheesh Elangovan, Lonnie Johnson, Karo Parsegian, Sangeetha Chandrasekaran

Periodontal diseases (PD) have been increasingly associated with several systemic conditions such as cardiovascular disease (CVD), diabetes mellitus (DM), rheumatoid arthritis (RA), and Alzheimer's disease (AD). This study aimed to gain insight into patients’ awareness of the association between PD and systemic diseases.

牙周疾病（PD）与心血管疾病（CVD）、糖尿病（DM）、类风湿性关节炎（RA）和阿尔茨海默病（AD）等多种全身性疾病的关联日益密切。本研究旨在深入了解患者对牙周病与全身性疾病之间关联的认识。

引用次数: 0

Association of periodontitis with gastrointestinal tract disorders: A bidirectional Mendelian randomization study 牙周炎与胃肠道疾病的关系：孟德尔随机双向研究

IF 4.2 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontology

Pub Date : 2024-04-02 DOI: 10.1002/JPER.23-0560

Yuqiang Wang, Jiakang Zhu, Ying Tang, Cui Huang

Background

The bidirectional link of periodontitis (PD) and gastrointestinal tract (GIT) disorders has been investigated in previous epidemiological studies; however, the conclusions still remain controversial. The aim of this study was to comprehensively explore the bidirectional causal effect between PD and various GIT diseases.

Methods

Based on summary-level data of genome-wide association studies (GWASs), a two-sample bidirectional Mendelian randomization (MR) study was undertaken. Single-nucleotide polymorphisms (SNPs) associated with PD or GIT disorders (chronic gastritis [CG], gastric ulcer [GU], duodenal ulcer [DU], gastroesophageal reflux disease [GERD], irritable bowel syndrome [IBS], and diverticular disease of the intestine [DI]) in GWASs were applied as exposure. The primary method employed was the inverse-variance weighted (IVW) method, and several sensitivity analyses were performed to investigate potential pleiotropy.

Results

With regard to the investigation of the causality between PD and GIT disorders, the IVW method revealed that there is a causal impact of PD on GU (odds ratio [OR] 1.088; 95% confidence interval [CI], 1.036–1.141; adjusted p = 0.004) and DI (OR 0.938; 95% CI, 0.911–0.965; adjusted p = 0.000). However, no significant genetic liability was observed for the causal effect of PD on CG, DU, GERD, and IBS. Furthermore, the primary analysis did not demonstrate a causal effect of GIT disorders on PD.

Conclusion

This MR study suggests that PD may be associated with an increased risk of GU and a reduced risk of DI, with possibly limited clinical relevance. Further studies are needed to support the conclusions of this MR study.

背景：以往的流行病学研究已对牙周炎（PD）与胃肠道疾病（GIT）的双向联系进行了调查，但结论仍存在争议。本研究旨在全面探讨牙周炎与各种胃肠道疾病之间的双向因果效应：方法：基于全基因组关联研究（GWAS）的摘要级数据，开展了一项双样本双向孟德尔随机化（MR）研究。将全基因组关联研究中与腹泻或胃肠道疾病（慢性胃炎[CG]、胃溃疡[GU]、十二指肠溃疡[DU]、胃食管反流病[GERD]、肠易激综合征[IBS]和肠憩室疾病[DI]）相关的单核苷酸多态性（SNPs）作为暴露。采用的主要方法是逆方差加权法（IVW），并进行了几种敏感性分析，以研究潜在的多重效应：关于腹泻症与消化道疾病之间因果关系的调查，IVW 方法显示腹泻症对 GU（几率比 [OR] 1.088；95% 置信区间 [CI]，1.036-1.141；调整后 p = 0.004）和 DI（OR 0.938；95% CI，0.911-0.965；调整后 p = 0.000）有因果影响。然而，在PD对CG、DU、胃食管反流病和肠易激综合征的因果效应方面，没有观察到明显的遗传责任。此外，主要分析也未显示胃肠道疾病对腹泻的因果效应：这项磁共振研究表明，PD 可能与 GU 风险增加和 DI 风险降低有关，但临床意义可能有限。需要进一步的研究来支持这项 MR 研究的结论。

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引用次数: 0