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Peri-implantitis induction and resolution around zirconia versus titanium implants. 氧化锆与钛种植体周围的种植体周围炎诱导与缓解。
IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-14 DOI: 10.1002/JPER.23-0573
Kaleb C Esplin, Yi-Wen Tsai, Kathryn Vela, Anibal Diogenes, Lea El Hachem, Archontia Palaiologou, David L Cochran, Georgios A Kotsakis

Background: This study compared titanium and zirconia implant ligature-induced peri-implant defect progression and response to regenerative surgical intervention.

Methods: Eight tissue-level endosseous implants were placed in 6 mixed-breed foxhounds, with 2 zirconia and 2 titanium alternating in each hemimandible. Cotton ligatures were placed subgingivally for 16 weeks followed by 8 weeks of spontaneous progression. Standardized radiographs were captured every 2 weeks to evaluate the rate of bone loss. Regenerative surgery was performed utilizing water-jet decontamination, enamel matrix derivative, and locally harvested autogenous bone. After 16 weeks of healing, final radiographic bone levels as well as probing depths, recession, and clinical attachment levels were assessed.

Results: All 48 implants integrated successfully. The final average post-ligature radiographic defects were 2.88 and 3.05 mm for titanium and zirconia implants, respectively. There was no significant difference between materials in the rate of radiographic bone loss (p = 0.09). Following regenerative surgery, the total average amount of radiographic bone gain was 1.41 and 1.20 mm for titanium and zirconia, respectively. The percentage of defect fill was 51.56% and 37.98% (p = 0.03) for titanium and zirconia, respectively. Inter-group differences were minimal for clinical parameters at the time of sacrifice including periodontal pocket depths (p = 0.81), recession (p = 0.98), or clinical attachment levels (p = 0.51).

Conclusions: No significant difference was found in the rate of peri-implant defect development between titanium and zirconia implants. Both materials gained significant radiographic bone following regenerative surgery with significantly greater defect percentage fill in titanium implants. The final clinical parameters were similar in both groups.

背景:本研究比较了钛和氧化锆种植体结扎引起的种植体周围缺损进展和对再生手术干预的反应:本研究比较了钛和氧化锆种植体结扎引起的种植体周围缺损进展以及对再生手术干预的反应:方法:在 6 只混种猎狐犬中植入 8 个组织水平的骨内种植体,每个半颌交替植入 2 个氧化锆和 2 个钛种植体。在龈下放置棉花结扎带 16 周,然后自发进行 8 周。每 2 周拍摄一次标准化 X 光片,以评估骨质流失率。利用喷水去污、釉质基质衍生物和当地采集的自体骨进行再生手术。愈合16周后,对最终的放射骨水平、探查深度、后退和临床附着水平进行评估:结果:所有 48 个种植体均成功整合。钛和氧化锆种植体最终的平均结扎后影像学缺损分别为 2.88 毫米和 3.05 毫米。不同材料的骨质流失率无明显差异(P = 0.09)。再生手术后,钛和氧化锆材料的放射骨增量总平均值分别为 1.41 毫米和 1.20 毫米。钛和氧化锆的缺损填充率分别为 51.56% 和 37.98%(p = 0.03)。牺牲时的临床参数,包括牙周袋深度(p = 0.81)、牙周退缩(p = 0.98)或临床附着水平(p = 0.51),组间差异极小:结论:钛和氧化锆种植体在种植体周围缺损发展率方面没有明显差异。两种材料在再生手术后都获得了明显的放射性骨量,但钛种植体的缺损填充率明显更高。两组的最终临床参数相似。
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引用次数: 0
Excessive weight in pregnant women increases the chance of diagnosing periodontitis combined with tooth loss. 孕妇体重过重会增加诊断牙周炎合并牙齿脱落的几率。
IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-10 DOI: 10.1002/JPER.23-0745
Josicélia Estrela Tuy Batista, Ana Claudia Morais Godoy Figueiredo, Elivan Silva Souza, Amanda Oliveira Lyrio, Johelle Santana Passos-Soares, Soraya Castro Trindade, Claudia Maria Coêlho Alves, Maria Isabel Pereira Vianna, Julita Maria Freitas Coelho, Sarah Dos Santos Conceição, Eneida de Moraes Marcílio Cerqueira, Alexandre Marcelo Hintz, Gregory John Seymour, Frank Andrew Scannapieco, Peter Michael Loomer, Isaac Suzart Gomes-Filho, Simone Seixas da Cruz

Background: Excess weight (EW), especially in women of childbearing age, those who are pregnant, as well as postpartum, is a problem worldwide. Fat accumulation deregulates the inflammatory response, contributing to the development of health problems, such as periodontitis. This study investigated the association between EW and periodontitis during pregnancy.

Methods: A cross-sectional, multicenter study involved 1745 postpartum women in Brazil. Socioeconomic-demographic data, gestational history, lifestyle behavior, and general and oral health conditions were obtained. Pre-pregnancy body mass index (BMI) was collected from medical records with EW being the exposure. Both tooth loss and clinical attachment level (CAL) were evaluated, and the presence of periodontitis was the outcome. Logistic regression, odds ratio (OR) and 95% confidence interval (95% CI), and quantile regression, beta coefficient and 95% CI, estimated the association between EW (BMI) and periodontitis and its combined effect with tooth loss ≥3, as dichotomous and continuous variables (CAL and tooth loss), with 5% significance level.

Results: The EW was 27.7% prevalent and periodontitis was 11.7%. There was a positive association between EW and periodontitis: ORadjusted:1.39; 95% CI:1.01;1.92 and between EW and periodontitis combined with tooth loss ≥3: ORadjusted:1.73; 95% CI:1.36;2.20. The adjusted association between EW and periodontitis as continuous variables was also positive, showing that for each unit of increased BMI, there was an elevation in the mean CAL (p = 0.04) and tooth loss (p < 0.01), with statistical significance.

Conclusions: There was a moderate association between EW and periodontitis during pregnancy, with an even greater association of pregnant women with EW presenting periodontitis combined with tooth loss.

背景:体重超标(EW),尤其是育龄妇女、孕妇和产后妇女的体重超标,是一个全球性问题。脂肪堆积会降低炎症反应,导致牙周炎等健康问题的发生。本研究调查了孕期 EW 与牙周炎之间的关系:这项横断面多中心研究涉及巴西的 1745 名产后妇女。获得了社会经济-人口学数据、妊娠史、生活方式、一般健康状况和口腔健康状况。从医疗记录中收集了孕前体重指数(BMI),并将 EW 作为暴露指标。对牙齿脱落和临床附着水平(CAL)进行评估,并以是否存在牙周炎作为结果。通过逻辑回归、几率比(OR)和 95% 置信区间(95% CI),以及量回归、贝塔系数和 95% CI,估算了 EW(BMI)与牙周炎之间的关系,以及其与牙齿脱落≥3(二分变量和连续变量(CAL 和牙齿脱落))之间的综合影响,显著性水平为 5%:结果:EW患病率为27.7%,牙周炎患病率为11.7%。EW与牙周炎呈正相关:EW与牙周炎之间存在正相关:OR调整值为1.39;95% CI:1.01;1.92;EW与牙周炎合并牙齿缺失≥3之间存在正相关:OR调整值为1.73;95% CI:1.36;2.20。作为连续变量,EW 与牙周炎之间的调整关联也是正相关的,表明体重指数每增加一个单位,平均 CAL(P = 0.04)和牙齿脱落(P 结论:EW 与牙周炎之间的关系是中度相关的:妊娠期 EW 与牙周炎之间存在中度关联,EW 孕妇合并牙周炎和牙齿脱落的关联更大。
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引用次数: 0
Association between nonalcoholic fatty liver disease scores and chronic periodontitis: A retrospective cohort study. 非酒精性脂肪肝评分与慢性牙周炎之间的关系:回顾性队列研究
IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-07 DOI: 10.1002/JPER.24-0171
Kwangmin Joo, Yeo Wool Kang, Sang Yi Moon, Yang Hyun Baek, Minkook Son

Background: Although several studies have demonstrated a bidirectional relationship between nonalcoholic fatty liver disease (NAFLD) and chronic periodontitis, few studies have reported that NAFLD causes chronic periodontitis, especially in the Asian population.

Methods: This study was conducted on 129,087 individuals, and the NAFLD score was assessed using the Fatty Liver Index (FLI), Hepatic Steatosis Index (HSI), and Framingham Steatosis Index (FSI). The incidence of chronic periodontitis was defined as a diagnostic code with dental procedures. Multi-variable adjusted Cox proportional hazard regression analysis was performed with hazard ratio (HR) and 95% confidence intervals (CIs).

Results: Nine thousand one hundred and twenty-eight chronic periodontitis cases (7.1%) were identified during a mean 7.4 years follow-up period. Each NAFLD score was related to chronic periodontitis. In the FLI score, HR and 95% CIs for the incidence of chronic periodontitis compared with a low FLI group were as follows: indeterminate FLI: 1.19 (1.12-1.26), high FLI: 1.32 (1.18-1.47). In the HSI and FSI scores, HR and 95% CIs for the incidence of chronic periodontitis were 1.13 (1.05-1.22) and 1.23 (1.05-1.31), respectively.

Conclusions: All NAFLD scores were associated with chronic periodontitis in the Korean population. As chronic periodontitis can aggravate the liver status, patients with NAFLD may need regular dental visits.

背景:尽管多项研究表明非酒精性脂肪肝与慢性牙周炎之间存在双向关系,但很少有研究报告称非酒精性脂肪肝会导致慢性牙周炎,尤其是在亚洲人群中:本研究以 129,087 人为对象,使用脂肪肝指数(FLI)、肝脏脂肪变性指数(HSI)和弗雷明汉脂肪变性指数(FSI)评估非酒精性脂肪肝得分。慢性牙周炎的发病率被定义为牙科手术的诊断代码。进行了多变量调整后的考克斯比例危险回归分析,并得出了危险比(HR)和 95% 的置信区间(CIs):结果:在平均 7.4 年的随访期间,共发现 9128 例慢性牙周炎病例(7.1%)。非酒精性脂肪肝的各项评分均与慢性牙周炎有关。在FLI评分中,与低FLI组相比,慢性牙周炎发病率的HR和95% CI如下:不确定FLI:1.19(1.12-1.26),高FLI:1.32(1.18-1.47)。在HSI和FSI评分中,慢性牙周炎发病率的HR和95% CI分别为1.13(1.05-1.22)和1.23(1.05-1.31):结论:在韩国人群中,所有非酒精性脂肪肝评分均与慢性牙周炎有关。由于慢性牙周炎会加重肝脏状况,非酒精性脂肪肝患者可能需要定期看牙医。
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引用次数: 0
Beyond transcription, aryl hydrocarbon receptor plays a protective role in periodontitis by interacting with CaMKII. 除转录外,芳基烃受体还通过与 CaMKII 相互作用,在牙周炎中发挥保护作用。
IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-05 DOI: 10.1002/JPER.24-0021
Xuwen Zeng, Meiting Feng, Jiawei Lu, Ruiling Wang, Li Deng, Yanan Yang, Lijun Luo

Background: The aryl hydrocarbon receptor (AhR) has been studied as an intracellular pattern recognition receptor that can identify bacterial pigments. To identify a potential therapeutic target for periodontitis, we investigated the expression of AhR in periodontitis and its role in the pathogenesis of periodontitis.

Methods: First, we analyzed AhR expression in a single-cell dataset from human periodontal tissue. Quantitative polymerase chain reaction (qPCR), immunofluorescence, and immunohistochemistry were used to verify the AhR level. Later, we determined the phenotypes of ligature-induced periodontitis in myeloid-specific AhR-deficient mice (Lyz2-Cre+/- AhRfx/fx), after which RNA sequencing (RNA-seq), qPCR, Western blot, immunofluorescence, and immunohistochemistry were used to investigate the impacts of AhR on periodontitis and its mechanism. Finally, we determined the therapeutic effect of AhR agonist 6-Formylindolo[3,2-b]carbazole (FICZ) administration on murine periodontitis and verified the effects of FICZ on macrophage polarization in vitro.

Results: AhR expression was enhanced in macrophages from periodontitis patients. Deletion of AhR from macrophages aggravated ligature-induced periodontitis and promoted the inflammatory response. Calcium/calmodulin-stimulated protein kinase II (CaMKII) phosphorylation was accelerated in AhR-deficient macrophages. Inhibiting CaMKII phosphorylation ameliorated periodontitis in Lyz2-Cre+/- AhRfx/fx mice. FICZ treatment blocked alveolar bone loss and relieved periodontal inflammation. FICZ diminished M1 macrophage polarization and promoted M2 macrophage polarization upon M1 macrophage induction.

Conclusion: AhR played a protective role in the pathogenesis of periodontitis by orchestrating macrophage polarization via interacting with the CaMKII signaling pathway.

背景:芳基烃受体(AhR)是一种细胞内模式识别受体,可以识别细菌色素。为了确定牙周炎的潜在治疗靶点,我们研究了 AhR 在牙周炎中的表达及其在牙周炎发病机制中的作用:首先,我们分析了人类牙周组织单细胞数据集中 AhR 的表达。定量聚合酶链反应(qPCR)、免疫荧光和免疫组化被用来验证 AhR 水平。随后,我们确定了髓细胞特异性 AhR 缺失小鼠(Lyz2-Cre+/- AhRfx/fx)结扎诱导牙周炎的表型,并利用 RNA 测序(RNA-seq)、qPCR、Western 印迹、免疫荧光和免疫组化等方法研究了 AhR 对牙周炎的影响及其机制。最后,我们确定了 AhR 激动剂 6-甲酰基吲哚并[3,2-b]咔唑(FICZ)对小鼠牙周炎的治疗效果,并在体外验证了 FICZ 对巨噬细胞极化的影响:结果:牙周炎患者的巨噬细胞中AhR表达增强。结果:牙周炎患者的巨噬细胞中 AhR 表达增强,巨噬细胞中 AhR 的缺失会加重结扎诱导的牙周炎并促进炎症反应。在 AhR 缺失的巨噬细胞中,钙/钙调蛋白激酶 II(CaMKII)磷酸化加速。抑制 CaMKII 磷酸化可改善 Lyz2-Cre+/- AhRfx/fx 小鼠的牙周炎。FICZ治疗可阻止牙槽骨流失并缓解牙周炎症。FICZ可减少M1巨噬细胞的极化,并在诱导M1巨噬细胞时促进M2巨噬细胞的极化:结论:AhR通过与CaMKII信号通路相互作用,协调巨噬细胞极化,从而在牙周炎的发病机制中发挥保护作用。
{"title":"Beyond transcription, aryl hydrocarbon receptor plays a protective role in periodontitis by interacting with CaMKII.","authors":"Xuwen Zeng, Meiting Feng, Jiawei Lu, Ruiling Wang, Li Deng, Yanan Yang, Lijun Luo","doi":"10.1002/JPER.24-0021","DOIUrl":"https://doi.org/10.1002/JPER.24-0021","url":null,"abstract":"<p><strong>Background: </strong>The aryl hydrocarbon receptor (AhR) has been studied as an intracellular pattern recognition receptor that can identify bacterial pigments. To identify a potential therapeutic target for periodontitis, we investigated the expression of AhR in periodontitis and its role in the pathogenesis of periodontitis.</p><p><strong>Methods: </strong>First, we analyzed AhR expression in a single-cell dataset from human periodontal tissue. Quantitative polymerase chain reaction (qPCR), immunofluorescence, and immunohistochemistry were used to verify the AhR level. Later, we determined the phenotypes of ligature-induced periodontitis in myeloid-specific AhR-deficient mice (Lyz2-Cre<sup>+/-</sup> AhR<sup>fx/fx</sup>), after which RNA sequencing (RNA-seq), qPCR, Western blot, immunofluorescence, and immunohistochemistry were used to investigate the impacts of AhR on periodontitis and its mechanism. Finally, we determined the therapeutic effect of AhR agonist 6-Formylindolo[3,2-b]carbazole (FICZ) administration on murine periodontitis and verified the effects of FICZ on macrophage polarization in vitro.</p><p><strong>Results: </strong>AhR expression was enhanced in macrophages from periodontitis patients. Deletion of AhR from macrophages aggravated ligature-induced periodontitis and promoted the inflammatory response. Calcium/calmodulin-stimulated protein kinase II (CaMKII) phosphorylation was accelerated in AhR-deficient macrophages. Inhibiting CaMKII phosphorylation ameliorated periodontitis in Lyz2-Cre<sup>+/-</sup> AhR<sup>fx/fx</sup> mice. FICZ treatment blocked alveolar bone loss and relieved periodontal inflammation. FICZ diminished M1 macrophage polarization and promoted M2 macrophage polarization upon M1 macrophage induction.</p><p><strong>Conclusion: </strong>AhR played a protective role in the pathogenesis of periodontitis by orchestrating macrophage polarization via interacting with the CaMKII signaling pathway.</p>","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gingipain and oncostatin M synergistically disrupt kidney tight junctions in periodontitis-associated acute kidney injury 在牙周炎相关急性肾损伤中,Gingipain 和 oncostatin M 能协同破坏肾脏紧密连接。
IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-04 DOI: 10.1002/JPER.24-0007
Wei Wei, Jing Sun, Zhaoxin Ji, Jiangqi Hu, Qingsong Jiang

Background

Acute kidney injury (AKI) is characterized by rapid renal decline. Periodontitis, a chronic oral inflammatory disease, is increasingly associated with renal dysfunction. Although periodontitis is recognized as a contributor to kidney damage, the mechanisms linking it to AKI remain unclear.

Methods

This study explored the effects of Porphyromonas gingivalis (P. gingivalis) W83-infected periodontitis on AKI in C57BL/6J mice, using ischemia-reperfusion injury 55 days post-infection. Gingipain inhibitors, KYT-1 and KYT-36, were applied. Detection of P. gingivalis was performed using quantitative real-time polymerase chain reaction (qRT-PCR) and PCR, while transcriptome sequencing, qRT-PCR, immunohistochemistry, and immunofluorescence staining assessed renal damage. In vitro, HK-2 cells were exposed to P. gingivalis at a multiplicity of infection of 10 for 48 h, with inhibition by gingipain or oncostatin M (OSM). Disruption of tight junctions (TJs) was quantified using qRT-PCR, transepithelial electrical resistance, and cell counting kit-8 assays.

Results

Periodontitis worsened AKI, linked to P. gingivalis infection and renal TJ disruption in the kidney. P. gingivalis infection activated OSM expression, which correlated positively with gingipain. Significantly, OSM and gingipain might collaboratively contribute to the damage of renal TJs, with the reduced expression of TJ proteins. Suppressing gingipain activity presented itself as a protective strategy against the destruction of TJs and the attendant worsening of AKI due to periodontitis.

Conclusions

Our study enhances the understanding of the interplay between periodontitis and AKI, highlighting the harmful impact of P. gingivalis in AKI.

背景:急性肾损伤(AKI)的特点是肾功能急剧下降。牙周炎是一种慢性口腔炎症性疾病,越来越多地与肾功能障碍相关。尽管牙周炎被认为是造成肾损伤的一个因素,但它与急性肾损伤的关联机制仍不清楚:本研究探讨了牙龈卟啉单胞菌(P. gingivalis)W83感染的牙周炎对C57BL/6J小鼠AKI的影响。应用牙龈蛋白酶抑制剂 KYT-1 和 KYT-36。使用定量实时聚合酶链式反应(qRT-PCR)和 PCR 检测牙龈脓疱病,同时使用转录组测序、qRT-PCR、免疫组织化学和免疫荧光染色评估肾损伤。在体外,HK-2细胞以10的感染倍数暴露于牙龈脓疱疮菌48小时,并受到gingipain或oncostatin M(OSM)的抑制。使用 qRT-PCR、跨上皮电阻和细胞计数试剂盒-8 检测法量化紧密连接(TJ)的破坏情况:结果:牙周炎加重了 AKI,这与牙龈脓胞感染和肾脏 TJ 破坏有关。牙龈脓疱疮感染激活了 OSM 的表达,而 OSM 的表达与 gingipain 呈正相关。值得注意的是,OSM和gingipain可能会共同导致肾脏TJ受损,同时降低TJ蛋白的表达。抑制gingipain的活性可作为一种保护性策略,防止TJ被破坏以及随之而来的牙周炎导致的AKI恶化:我们的研究加深了人们对牙周炎与 AKI 之间相互作用的理解,突出了牙龈脓疱病在 AKI 中的有害影响。
{"title":"Gingipain and oncostatin M synergistically disrupt kidney tight junctions in periodontitis-associated acute kidney injury","authors":"Wei Wei,&nbsp;Jing Sun,&nbsp;Zhaoxin Ji,&nbsp;Jiangqi Hu,&nbsp;Qingsong Jiang","doi":"10.1002/JPER.24-0007","DOIUrl":"10.1002/JPER.24-0007","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Acute kidney injury (AKI) is characterized by rapid renal decline. Periodontitis, a chronic oral inflammatory disease, is increasingly associated with renal dysfunction. Although periodontitis is recognized as a contributor to kidney damage, the mechanisms linking it to AKI remain unclear.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study explored the effects of <i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>) W83-infected periodontitis on AKI in C57BL/6J mice, using ischemia-reperfusion injury 55 days post-infection. Gingipain inhibitors, KYT-1 and KYT-36, were applied. Detection of <i>P. gingivalis</i> was performed using quantitative real-time polymerase chain reaction (qRT-PCR) and PCR, while transcriptome sequencing, qRT-PCR, immunohistochemistry, and immunofluorescence staining assessed renal damage. In vitro, HK-2 cells were exposed to <i>P. gingivalis</i> at a multiplicity of infection of 10 for 48 h, with inhibition by gingipain or oncostatin M (OSM). Disruption of tight junctions (TJs) was quantified using qRT-PCR, transepithelial electrical resistance, and cell counting kit-8 assays.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Periodontitis worsened AKI, linked to <i>P. gingivalis</i> infection and renal TJ disruption in the kidney. <i>P. gingivalis</i> infection activated OSM expression, which correlated positively with gingipain. Significantly, OSM and gingipain might collaboratively contribute to the damage of renal TJs, with the reduced expression of TJ proteins. Suppressing gingipain activity presented itself as a protective strategy against the destruction of TJs and the attendant worsening of AKI due to periodontitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our study enhances the understanding of the interplay between periodontitis and AKI, highlighting the harmful impact of <i>P. gingivalis</i> in AKI.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/JPER.24-0007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
How well do antimicrobial mouth rinses prevent dysbiosis in an in vitro periodontitis biofilm model? 抗菌漱口水在体外牙周炎生物膜模型中预防菌群失调的效果如何?
IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-30 DOI: 10.1002/JPER.23-0674
Naiera Zayed, Rik Vertommen, Kenneth Simoens, Kristel Bernaerts, Nico Boon, Mrinal Gaurav Srivastava, Annabel Braem, Wannes Van Holm, Ana B. Castro, Wim Teughels

Background

Periodontal diseases are associated with dysbiosis in the oral microbial communities. Managing oral biofilms is therefore key for preventing these diseases. Management protocols often include over-the-counter antimicrobial mouth rinses, which lack data on their effects on the oral microbiome's ecology, bacterial composition, metabolic activity, and dysbiosis resilience. This study examined the efficacy of antimicrobial mouth rinses to halt dysbiosis in in vitro oral biofilms under periodontitis-simulating conditions.

Methods

Multispecies oral biofilms were grown on hydroxyapatite discs (HADs) and rinsed daily with one of six mouth rinses. Positive and negative controls were included. After three rinses, biofilms were analyzed with viability quantitative polymerase chain reaction and visualized using scanning electron microscopy. Supernatants of rinsed biofilms were used for metabolic activity analysis. In addition, human oral keratinocytes were exposed to rinsed biofilms to assess their inflammatory response. All outputs were analyzed for correlation using Spearman coefficient.

Results

Product-related changes were observed in the rinsed biofilms. Three of the six tested mouth rinses could significantly prevent dysbiosis with ≥30% reduction in pathobiont abundance relative to the control. These biofilms had lower metabolic activity, and the exposed human oral keratinocyte produced less interleukin-8. Interleukin-8 production correlated to both pathobiont quantity and the metabolic activity of the biofilms.

Conclusion

Some mouth rinses could support biofilm resilience and stop dysbiosis evolution in the biofilm model, with a clear product-related effect. Such mouth rinses can be considered for patients under maintenance/supportive periodontal therapy to prevent/delay disease recurrence. Others are more useful for different periodontal therapy stages.

背景:牙周疾病与口腔微生物群落的菌群失调有关。因此,管理口腔生物膜是预防这些疾病的关键。管理方案通常包括非处方抗菌漱口水,但缺乏有关这些漱口水对口腔微生物群生态、细菌组成、代谢活动和菌群失调恢复能力的影响的数据。本研究考察了抗菌漱口水在模拟牙周炎的体外口腔生物膜中阻止菌群失调的功效:多菌种口腔生物膜生长在羟基磷灰石盘(HAD)上,每天用六种漱口水中的一种漱口。其中包括阳性对照组和阴性对照组。漱口三次后,用活力定量聚合酶链反应分析生物膜,并用扫描电子显微镜观察。冲洗后的生物膜上清液用于代谢活性分析。此外,还将人类口腔角质细胞暴露于冲洗过的生物膜,以评估其炎症反应。所有结果均使用斯皮尔曼系数进行相关性分析:结果:在冲洗过的生物膜中观察到了与产品相关的变化。在六种测试的漱口水中,有三种能显著预防菌群失调,与对照组相比,病原体丰度降低了≥30%。这些生物膜的代谢活性较低,暴露的人类口腔角质细胞产生的白细胞介素-8较少。白细胞介素-8的产生与病原菌的数量和生物膜的代谢活性有关:结论:一些漱口水可以支持生物膜的恢复能力,并阻止生物膜模型中菌群失调的演变,其效果明显与产品有关。接受维护性/支持性牙周治疗的患者可以考虑使用这类漱口水,以预防/延缓疾病复发。其他漱口水则更适用于不同的牙周治疗阶段。
{"title":"How well do antimicrobial mouth rinses prevent dysbiosis in an in vitro periodontitis biofilm model?","authors":"Naiera Zayed,&nbsp;Rik Vertommen,&nbsp;Kenneth Simoens,&nbsp;Kristel Bernaerts,&nbsp;Nico Boon,&nbsp;Mrinal Gaurav Srivastava,&nbsp;Annabel Braem,&nbsp;Wannes Van Holm,&nbsp;Ana B. Castro,&nbsp;Wim Teughels","doi":"10.1002/JPER.23-0674","DOIUrl":"10.1002/JPER.23-0674","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Periodontal diseases are associated with dysbiosis in the oral microbial communities. Managing oral biofilms is therefore key for preventing these diseases. Management protocols often include over-the-counter antimicrobial mouth rinses, which lack data on their effects on the oral microbiome's ecology, bacterial composition, metabolic activity, and dysbiosis resilience. This study examined the efficacy of antimicrobial mouth rinses to halt dysbiosis in in vitro oral biofilms under periodontitis-simulating conditions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Multispecies oral biofilms were grown on hydroxyapatite discs (HADs) and rinsed daily with one of six mouth rinses. Positive and negative controls were included. After three rinses, biofilms were analyzed with viability quantitative polymerase chain reaction and visualized using scanning electron microscopy. Supernatants of rinsed biofilms were used for metabolic activity analysis. In addition, human oral keratinocytes were exposed to rinsed biofilms to assess their inflammatory response. All outputs were analyzed for correlation using Spearman coefficient.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Product-related changes were observed in the rinsed biofilms. Three of the six tested mouth rinses could significantly prevent dysbiosis with ≥30% reduction in pathobiont abundance relative to the control. These biofilms had lower metabolic activity, and the exposed human oral keratinocyte produced less interleukin-8. Interleukin-8 production correlated to both pathobiont quantity and the metabolic activity of the biofilms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Some mouth rinses could support biofilm resilience and stop dysbiosis evolution in the biofilm model, with a clear product-related effect. Such mouth rinses can be considered for patients under maintenance/supportive periodontal therapy to prevent/delay disease recurrence. Others are more useful for different periodontal therapy stages.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/JPER.23-0674","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Laser and scanning electron microscopy evaluation of residual microislands of calculus 用激光和扫描电子显微镜评估牙结石残留微区
IF 4.3 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-28 DOI: 10.1002/jper.24-0191
Stephen K. Harrel, Charles M. Cobb, Martha E. Nunn, Donggao Zhao
BackgroundRecent studies suggest a role for microscopic crystalline particles of residual dental calculus in the pathogenesis of periodontitis. The purpose of this ex vivo study was to compare the effectiveness of scaling and root planing (SRP) alone versus SRP combined with 24% ethylenediamine‐tetra acetic acid (EDTA) gel in removing calculus from extracted teeth and to determine the optimal length of time for application of the EDTA.MethodsSpecimens consisted of 32 extracted teeth with heavy root calculus. A 4‐mm diameter site was prepared on the root surface of each tooth which then underwent SRP. EDTA was applied to four timed groups: 30 s; 60 s; 120 s; and 180 s. Photomicrographs were taken at 40× magnification using white light (WL) and laser fluorescence (LF). Photomicrographs were analyzed using ImageJ. Specimens were also evaluated with scanning electron microscopy (SEM).ResultsThe mean area of residual calculus after SRP was 45%–53% (45.6% ± 19.6% WL, 53.8% ± 19.7% LF). Burnishing with EDTA for one minute following SRP reduced calculus to only 14%–18% (13.9% ± 12.5% LF, 18.2% ± 11.1% WL). Use of EDTA for greater than 1 min showed no further calculus removal. SEM revealed the surface of remaining calculus was altered by burnishing with EDTA.ConclusionSRP alone or SRP + 24% EDTA gel failed to remove all calculus. SRP alone removed >60% of calculus from root surfaces. Adjunctive use of 24% EDTA gel burnished on the root surface removed most of the calculus residual after SRP. Calculus remaining after EDTA burnishing exhibited a significantly altered morphologic appearance.
背景最近的研究表明,残留牙结石的微小结晶颗粒在牙周炎的发病机制中扮演着重要角色。这项体内外研究的目的是比较单纯洗牙和根面平整(SRP)与结合 24% 乙二胺四乙酸(EDTA)凝胶的 SRP 在去除拔牙结石方面的效果,并确定使用 EDTA 的最佳时间长度。在每颗牙齿的根部表面制备一个直径为 4 毫米的部位,然后进行 SRP。使用白光(WL)和激光荧光(LF)以 40 倍放大率拍摄显微照片。显微照片使用 ImageJ 进行分析。结果SRP后残留结石的平均面积为45%-53%(45.6% ± 19.6% WL,53.8% ± 19.7% LF)。SRP 后用 EDTA 烧灼一分钟,结石仅减少了 14%-18%(13.9% ± 12.5% LF,18.2% ± 11.1% WL)。使用 EDTA 超过 1 分钟后,牙结石没有进一步去除。扫描电子显微镜显示,剩余结石的表面因使用 EDTA 烧灼而发生了改变。单独使用 SRP 清除了牙根表面 60% 的牙结石。辅助使用 24% EDTA 凝胶烧灼牙根表面,可去除 SRP 后残留的大部分牙结石。EDTA 烧灼后残留的牙结石在形态上有明显的改变。
{"title":"Laser and scanning electron microscopy evaluation of residual microislands of calculus","authors":"Stephen K. Harrel, Charles M. Cobb, Martha E. Nunn, Donggao Zhao","doi":"10.1002/jper.24-0191","DOIUrl":"https://doi.org/10.1002/jper.24-0191","url":null,"abstract":"BackgroundRecent studies suggest a role for microscopic crystalline particles of residual dental calculus in the pathogenesis of periodontitis. The purpose of this ex vivo study was to compare the effectiveness of scaling and root planing (SRP) alone versus SRP combined with 24% ethylenediamine‐tetra acetic acid (EDTA) gel in removing calculus from extracted teeth and to determine the optimal length of time for application of the EDTA.MethodsSpecimens consisted of 32 extracted teeth with heavy root calculus. A 4‐mm diameter site was prepared on the root surface of each tooth which then underwent SRP. EDTA was applied to four timed groups: 30 s; 60 s; 120 s; and 180 s. Photomicrographs were taken at 40× magnification using white light (WL) and laser fluorescence (LF). Photomicrographs were analyzed using ImageJ. Specimens were also evaluated with scanning electron microscopy (SEM).ResultsThe mean area of residual calculus after SRP was 45%–53% (45.6% ± 19.6% WL, 53.8% ± 19.7% LF). Burnishing with EDTA for one minute following SRP reduced calculus to only 14%–18% (13.9% ± 12.5% LF, 18.2% ± 11.1% WL). Use of EDTA for greater than 1 min showed no further calculus removal. SEM revealed the surface of remaining calculus was altered by burnishing with EDTA.ConclusionSRP alone or SRP + 24% EDTA gel failed to remove all calculus. SRP alone removed &gt;60% of calculus from root surfaces. Adjunctive use of 24% EDTA gel burnished on the root surface removed most of the calculus residual after SRP. Calculus remaining after EDTA burnishing exhibited a significantly altered morphologic appearance.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141463118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harvest of epithelialized gingival grafts without application of hemostatic sutures: A randomized clinical trial using laser speckle contrast imaging 无需止血缝合即可收获上皮化牙龈移植物:使用激光斑点对比成像的随机临床试验
IF 4.3 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-28 DOI: 10.1002/jper.23-0620
David Palombo, Andrea Dobos, Mercedes Lopez Duran, Javier Sanz Esporrin, Mariano Sanz
BackgroundThis randomized controlled trial with two parallel arms and a 1:1 allocation ratio aimed to compare early microvascular healing (primary outcome), surgical times, and patient‐reported outcomes (PROM) after harvesting palatal epithelialized gingival grafts (EGG), where hemostasis was achieved with sutures and hemostatic sponges (control) or with a sutureless approach (test).MethodsFrom a total of 33 patients, 34 EGG were harvested. Thirty‐two were randomized to the test/control group (n = 16) and two were excluded. Early palatal microvascular healing was assessed at 7, 14, and 30 days with laser speckle contrast imaging (LSCI). Postoperative bleeding, pain, discomfort, and analgesic consumption were assessed over 2 weeks with a dedicated questionnaire.ResultsA faster onset and resolution of postharvest hyperemia was observed in the test group where peak blood flow was reached at 7 days. No significant blood flow differences were observed between the groups at any of the evaluated timepoints. The mean surgical time was 13 min shorter in the test (p = 0.00). No significant differences were observed for postoperative bleeding and analgesic consumption at any timepoint.ConclusionsThe tested approach represents a viable alternative to the standard one, providing no relevant differences in microvascular, clinical, and patient‐related results, but with significantly shorter surgical times.
背景这项随机对照试验有两个平行臂,分配比例为 1:1,旨在比较腭上皮化生牙龈移植物(EGG)采集后的早期微血管愈合(主要结果)、手术时间和患者报告结果(PROM),其中采用缝合和止血海绵止血(对照组)或采用无缝合方法止血(试验组)。32人被随机分配到试验/对照组(n = 16),2人被排除在外。通过激光斑点对比成像(LSCI)在 7、14 和 30 天评估腭部早期微血管愈合情况。使用专用问卷对术后两周内的出血、疼痛、不适和镇痛药消耗量进行评估。结果 在试验组观察到收获后充血开始和消退较快,7 天时血流达到峰值。在任何一个评估时间点,两组之间均未观察到明显的血流差异。试验组的平均手术时间缩短了 13 分钟(P = 0.00)。结论试验方法是标准方法的可行替代方案,在微血管、临床和患者相关结果方面没有相关差异,但手术时间明显缩短。
{"title":"Harvest of epithelialized gingival grafts without application of hemostatic sutures: A randomized clinical trial using laser speckle contrast imaging","authors":"David Palombo, Andrea Dobos, Mercedes Lopez Duran, Javier Sanz Esporrin, Mariano Sanz","doi":"10.1002/jper.23-0620","DOIUrl":"https://doi.org/10.1002/jper.23-0620","url":null,"abstract":"BackgroundThis randomized controlled trial with two parallel arms and a 1:1 allocation ratio aimed to compare early microvascular healing (primary outcome), surgical times, and patient‐reported outcomes (PROM) after harvesting palatal epithelialized gingival grafts (EGG), where hemostasis was achieved with sutures and hemostatic sponges (control) or with a sutureless approach (test).MethodsFrom a total of 33 patients, 34 EGG were harvested. Thirty‐two were randomized to the test/control group (<jats:italic>n</jats:italic> = 16) and two were excluded. Early palatal microvascular healing was assessed at 7, 14, and 30 days with laser speckle contrast imaging (LSCI). Postoperative bleeding, pain, discomfort, and analgesic consumption were assessed over 2 weeks with a dedicated questionnaire.ResultsA faster onset and resolution of postharvest hyperemia was observed in the test group where peak blood flow was reached at 7 days. No significant blood flow differences were observed between the groups at any of the evaluated timepoints. The mean surgical time was 13 min shorter in the test (<jats:italic>p</jats:italic> = 0.00). No significant differences were observed for postoperative bleeding and analgesic consumption at any timepoint.ConclusionsThe tested approach represents a viable alternative to the standard one, providing no relevant differences in microvascular, clinical, and patient‐related results, but with significantly shorter surgical times.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141462809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cystathionine γ‐lyase contributes to exacerbation of periodontal destruction in experimental periodontitis under hyperglycemia 胱硫醚 γ-lyase 在高血糖实验性牙周炎中加剧了牙周破坏
IF 4.3 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-28 DOI: 10.1002/jper.23-0811
Danni Song, Jiangfeng He, Tianfan Cheng, Lijian Jin, Sijin Li, Beibei Chen, Yongming Li, Chongshan Liao
BackgroundDiabetes is one of the major inflammatory comorbidities of periodontitis via 2‐way interactions. Cystathionine γ‐lyase (CTH) is a pivotal endogenous enzyme synthesizing hydrogen sulfide (H2S), and CTH/H2S is crucially implicated in modulating inflammation in various diseases. This study aimed to explore the potential role of CTH in experimental periodontitis under a hyperglycemic condition.MethodsCTH‐silenced and normal human periodontal ligament cells (hPDLCs) were cultured in a high glucose and Porphyromonas gingivalis lipopolysaccharide (P.g‐LPS) condition. The effects of CTH on hPDLCs were assessed by Cell Counting Kit 8 (CCK8), real‐time quantitative polymerase chain reaction (RT‐qPCR), and enzyme‐linked immunosorbent assay (ELISA). The model of experimental periodontitis under hyperglycemia was established on both Cth−/− and wild‐type (WT) mice, and the extent of periodontal destruction was assessed by micro‐CT, histology, RNA‐Seq, Western blot, tartrate‐resistant acid phosphatase (TRAP) staining and immunostaining.ResultsCTH mRNA expression increased in hPDLCs in response to increasing concentration of P.g‐LPS stimulation in a high glucose medium. With reference to WT mice, Cth−/− mice with experimental periodontitis under hyperglycemia exhibited reduced bone loss, decreased leukocyte infiltration and hindered osteoclast formation, along with reduced expression of proinflammatory cytokines interleukin‐6 (IL‐6) and tumor necrosis factor alpha (TNF‐α) in periodontal tissue. RNA‐seq‐enriched altered NF‐κB pathway signaling in healthy murine gingiva with experimental periodontitis mice under hyperglycemia. Accordingly, phosphorylation of p65 (P‐p65) was alleviated in CTH‐silenced hPDLCs, leading to decreased expression of IL6 and TNF. CTH knockdown inhibited activation of nuclear factor kappa‐B (NF‐κB) pathway and decreased production of proinflammatory cytokines under high glucose and P.g‐LPS treatment.ConclusionThe present findings suggest the potential of CTH as a therapeutic target for tackling periodontitis in diabetic patients.
背景糖尿病是牙周炎的主要炎症并发症之一,与牙周炎有双向作用。胱硫醚γ-裂解酶(CTH)是一种合成硫化氢(H2S)的关键内源性酶,CTH/H2S在多种疾病的炎症调节中起着至关重要的作用。本研究旨在探讨高血糖条件下 CTH 在实验性牙周炎中的潜在作用。方法在高糖和牙龈卟啉菌脂多糖(P.g-LPS)条件下培养 CTH 沉默的正常人牙周韧带细胞(hPDLCs)。通过细胞计数试剂盒 8(CCK8)、实时定量聚合酶链反应(RT-qPCR)和酶联免疫吸附试验(ELISA)评估了 CTH 对 hPDLCs 的影响。在 Cth-/- 和野生型(WT)小鼠身上建立了高血糖条件下的实验性牙周炎模型,并通过显微 CT、组织学、RNA-Seq、Western 印迹、抗酒石酸磷酸酶(TRAP)染色和免疫染色来评估牙周破坏的程度。与 WT 小鼠相比,在高血糖条件下患实验性牙周炎的 Cth-/- 小鼠骨质流失减少,白细胞浸润减少,破骨细胞形成受阻,牙周组织中促炎性细胞因子白细胞介素-6(IL-6)和肿瘤坏死因子α(TNF-α)的表达减少。RNA-seq富集改变了健康小鼠牙龈与高血糖实验性牙周炎小鼠的NF-κB通路信号传导。因此,CTH沉默的hPDLCs中p65(P-p65)的磷酸化减轻,导致IL6和TNF的表达减少。CTH敲除抑制了核因子卡巴-B(NF-κB)通路的激活,并减少了高糖和P.g-LPS处理下促炎细胞因子的产生。
{"title":"Cystathionine γ‐lyase contributes to exacerbation of periodontal destruction in experimental periodontitis under hyperglycemia","authors":"Danni Song, Jiangfeng He, Tianfan Cheng, Lijian Jin, Sijin Li, Beibei Chen, Yongming Li, Chongshan Liao","doi":"10.1002/jper.23-0811","DOIUrl":"https://doi.org/10.1002/jper.23-0811","url":null,"abstract":"BackgroundDiabetes is one of the major inflammatory comorbidities of periodontitis via 2‐way interactions. Cystathionine γ‐lyase (CTH) is a pivotal endogenous enzyme synthesizing hydrogen sulfide (H<jats:sub>2</jats:sub>S), and CTH/H<jats:sub>2</jats:sub>S is crucially implicated in modulating inflammation in various diseases. This study aimed to explore the potential role of CTH in experimental periodontitis under a hyperglycemic condition.MethodsCTH‐silenced and normal human periodontal ligament cells (hPDLCs) were cultured in a high glucose and <jats:italic>Porphyromonas gingivalis</jats:italic> lipopolysaccharide (<jats:italic>P.g</jats:italic>‐LPS) condition. The effects of CTH on hPDLCs were assessed by Cell Counting Kit 8 (CCK8), real‐time quantitative polymerase chain reaction (RT‐qPCR), and enzyme‐linked immunosorbent assay (ELISA). The model of experimental periodontitis under hyperglycemia was established on both <jats:italic>Cth</jats:italic><jats:sup>−/−</jats:sup> and wild‐type (WT) mice, and the extent of periodontal destruction was assessed by micro‐CT, histology, RNA‐Seq, Western blot, tartrate‐resistant acid phosphatase (TRAP) staining and immunostaining.Results<jats:italic>CTH</jats:italic> mRNA expression increased in hPDLCs in response to increasing concentration of <jats:italic>P.g</jats:italic>‐LPS stimulation in a high glucose medium. With reference to WT mice<jats:italic>, Cth</jats:italic><jats:sup>−/−</jats:sup> mice with experimental periodontitis under hyperglycemia exhibited reduced bone loss, decreased leukocyte infiltration and hindered osteoclast formation, along with reduced expression of proinflammatory cytokines interleukin‐6 (IL‐6) and tumor necrosis factor alpha (TNF‐α) in periodontal tissue. RNA‐seq‐enriched altered NF‐κB pathway signaling in healthy murine gingiva with experimental periodontitis mice under hyperglycemia. Accordingly, phosphorylation of p65 (P‐p65) was alleviated in <jats:italic>CTH</jats:italic>‐silenced hPDLCs, leading to decreased expression of <jats:italic>IL6</jats:italic> and <jats:italic>TNF</jats:italic>. <jats:italic>CTH</jats:italic> knockdown inhibited activation of nuclear factor kappa‐B (NF‐κB) pathway and decreased production of proinflammatory cytokines under high glucose and <jats:italic>P.g</jats:italic>‐LPS treatment.ConclusionThe present findings suggest the potential of CTH as a therapeutic target for tackling periodontitis in diabetic patients.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141463085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Long‐term success and influencing factors of regenerative surgery for intra‐bony defects: A retrospective cohort study 骨内缺损再生手术的长期成功和影响因素:回顾性队列研究
IF 4.3 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-28 DOI: 10.1002/jper.23-0701
Yan Huang, Xiangying Ouyang, Bei Liu, Jianru Liu, Wenyi Liu, Ying Xie
BackgroundThe composite outcome measure (COM) more comprehensively assesses the clinical efficacy of regenerative surgery than a single probing measurement. We aimed to assess long‐term success defined by the COM (clinical attachment level [CAL] gain of ≥3 mm and postsurgery probing pocket depth [PPD] ≤ 4 mm) and influencing factors of regenerative surgery using bone substitutes and resorbable collagen membrane (RM) for intra‐bony defects (IBDs).MethodsWe retrospectively collected data from patients who underwent regenerative surgery using deproteinized bovine bone mineral (DBBM) and RM for IBDs. CAL and PPD values were compared at baseline (preoperative), 1 year (short‐term), and at the last follow‐up (5–10 years). Multivariate logistic regressions were performed to identify factors influencing COM‐based long‐term success.ResultsEighty‐one defects in 75 teeth of 33 patients who completed follow‐up (6.5 ± 1.4 years) were included. One tooth was lost. All defects with complete follow‐up exhibited long‐term average CAL gain (3.00 ± 2.00 mm, 95% confidence interval [CI]: 2.56–3.44 mm, p < 0.001) and PPD reduction (2.06 ± 1.91 mm, 95% CI: 1.64–2.49 mm, p < 0.001). Long‐term success was achieved in 38.8% of IBDs. CAL and PPD values were comparable between 1 year and the last follow‐up. Logistic regression analyses revealed that male sex (odds ratio [OR] = 0.23, 95% CI: 0.07–0.75) and bleeding on probing (BOP) during supportive periodontal therapy (OR = 0.96, 95% CI: 0.94–0.99) were risk factors for long‐term success.ConclusionsRegenerative surgery with DBBM and RM for IBDs can achieve some degree of long‐term success defined by COM. However, within this study's limitations, male sex and higher BOP incidence postoperatively are negatively associated with optimal long‐term success.Clinical trial numberChiCTR2300069016.
背景综合结果测量法(COM)比单一探查测量法更能全面评估再生手术的临床疗效。方法我们回顾性地收集了使用去蛋白牛骨矿物质(DBBM)和可吸收胶原膜(RM)进行骨内缺损(IBD)再生手术患者的数据。比较了基线(术前)、1 年(短期)和最后一次随访(5-10 年)时的 CAL 和 PPD 值。结果 共纳入 33 名完成随访(6.5 ± 1.4 年)的患者的 75 颗牙齿中的 81 处缺损。其中一颗牙齿脱落。所有完成随访的缺损均表现出长期平均 CAL 增加(3.00 ± 2.00 mm,95% 置信区间 [CI]:2.56-3.44 mm,p < 0.001)和 PPD 减少(2.06 ± 1.91 mm,95% CI:1.64-2.49 mm,p < 0.001)。38.8%的 IBD 获得了长期成功。CAL值和PPD值在1年和最后一次随访期间具有可比性。逻辑回归分析表明,男性(几率比[OR] = 0.23,95% CI:0.07-0.75)和牙周支持治疗期间的探诊出血(BOP)(OR = 0.96,95% CI:0.94-0.99)是长期成功的风险因素。临床试验编号:ChiCTR2300069016.
{"title":"Long‐term success and influencing factors of regenerative surgery for intra‐bony defects: A retrospective cohort study","authors":"Yan Huang, Xiangying Ouyang, Bei Liu, Jianru Liu, Wenyi Liu, Ying Xie","doi":"10.1002/jper.23-0701","DOIUrl":"https://doi.org/10.1002/jper.23-0701","url":null,"abstract":"BackgroundThe composite outcome measure (COM) more comprehensively assesses the clinical efficacy of regenerative surgery than a single probing measurement. We aimed to assess long‐term success defined by the COM (clinical attachment level [CAL] gain of ≥3 mm and postsurgery probing pocket depth [PPD] ≤ 4 mm) and influencing factors of regenerative surgery using bone substitutes and resorbable collagen membrane (RM) for intra‐bony defects (IBDs).MethodsWe retrospectively collected data from patients who underwent regenerative surgery using deproteinized bovine bone mineral (DBBM) and RM for IBDs. CAL and PPD values were compared at baseline (preoperative), 1 year (short‐term), and at the last follow‐up (5–10 years). Multivariate logistic regressions were performed to identify factors influencing COM‐based long‐term success.ResultsEighty‐one defects in 75 teeth of 33 patients who completed follow‐up (6.5 ± 1.4 years) were included. One tooth was lost. All defects with complete follow‐up exhibited long‐term average CAL gain (3.00 ± 2.00 mm, 95% confidence interval [CI]: 2.56–3.44 mm, <jats:italic>p </jats:italic>&lt; 0.001) and PPD reduction (2.06 ± 1.91 mm, 95% CI: 1.64–2.49 mm, <jats:italic>p </jats:italic>&lt; 0.001). Long‐term success was achieved in 38.8% of IBDs. CAL and PPD values were comparable between 1 year and the last follow‐up. Logistic regression analyses revealed that male sex (odds ratio [OR] = 0.23, 95% CI: 0.07–0.75) and bleeding on probing (BOP) during supportive periodontal therapy (OR = 0.96, 95% CI: 0.94–0.99) were risk factors for long‐term success.ConclusionsRegenerative surgery with DBBM and RM for IBDs can achieve some degree of long‐term success defined by COM. However, within this study's limitations, male sex and higher BOP incidence postoperatively are negatively associated with optimal long‐term success.Clinical trial numberChiCTR2300069016.","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141462904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Journal of periodontology
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