Laura Massarenti, Claus Henrik Nielsen, Anne Katrine Danielsen, Peter Østrup Jensen, Christian Enevold, Christian Damgaard
Background: Increasing evidence indicates that periodontitis contributes to systemic low-grade inflammation. Porphyromonas gingivalis is strongly associated with periodontitis, and antibodies against the bacterium may be used as a serological proxy to account for periodontal status, when studying diseases associated with periodontitis. The aim of the present study is to identify an easily accessible and reliable serological biomarker for determination of periodontal status and oral carriage of the bacterium.
Methods: Saliva and serum samples were collected from periodontally healthy controls (n = 27), and patients with periodontitis stage II (n = 12) or stages III or IV (n = 44). Serum levels of immunoglobulin G (IgG) antibodies against intact and fragmented P. gingivalis, recombinant gingipains (RgpA and RgpB), and the bacteria Escherichia coli and Capnocytophaga ochracea as controls were quantified with a multiplex bead-based assay. P. gingivalis was identified in saliva using quantitative polymerase chain reaction (qPCR).
Results: Serum IgG antibodies against P. gingivalis whole bacteria were good indicators of periodontitis (area under the curve [AUC]: 0.75, 95% confidence interval [CI]: 0.64-0.85). The same was observed for levels of antibodies against P. gingivalis fragments (AUC: 0.78, 95% CI: 0.68-0.88). Likewise, levels of antibodies against P. gingivalis whole bacteria or P. gingivalis fragments were good indicators of oral carriage of P. gingivalis (AUC: 0.92, 95% CI: 0.86-0.98 and AUC: 0.96, 95% CI: 0.92-1, respectively). Conversely, antibodies against recombinant RgpA and RgpB were not good indicators of periodontitis or oral carriage of the bacterium. None of the antibody levels differed significantly between stage II and stage III or IV periodontitis.
Conclusion: Serum IgG antibody levels against heat-inactivated whole P. gingivalis proved to be the preferable biomarker for periodontitis and oral carriage of the bacterium.
背景:越来越多的证据表明,牙周炎会导致全身性低度炎症。牙龈卟啉单胞菌(Porphyromonas gingivalis)与牙周炎密切相关,在研究与牙周炎相关的疾病时,针对牙龈卟啉单胞菌的抗体可作为一种血清学替代物来说明牙周状况。本研究的目的是确定一种易于获得且可靠的血清学生物标志物,用于确定牙周状况和该细菌的口腔携带情况:方法:从牙周健康的对照组(27 人)和牙周炎 II 期(12 人)、III 期或 IV 期(44 人)患者中采集唾液和血清样本。采用基于多聚酶珠的检测方法对血清中针对完整和片段牙龈脓杆菌、重组牙龈肽(RgpA 和 RgpB)以及作为对照的大肠杆菌和嗜口荚膜梭菌的免疫球蛋白 G (IgG) 抗体水平进行量化。使用定量聚合酶链反应(qPCR)鉴定唾液中的牙龈脓疱疮杆菌:结果:血清中针对牙龈脓疱病菌的 IgG 抗体是牙周炎的良好指标(曲线下面积 [AUC]:0.75,95% 置信区间 [CI]:0.64-0.85)。针对牙龈片段的抗体水平也是如此(AUC:0.78,95% 置信区间:0.68-0.88)。同样,针对牙龈脓杆菌全菌或牙龈脓杆菌片段的抗体水平也是牙龈脓杆菌口腔携带的良好指标(AUC:0.92,95% CI:0.86-0.98;AUC:0.96,95% CI:0.92-1)。相反,针对重组 RgpA 和 RgpB 的抗体不是牙周炎或口腔带菌的良好指标。II期牙周炎与III期或IV期牙周炎的抗体水平均无明显差异:结论:针对热灭活全牙龈脓毒性杆菌的血清 IgG 抗体水平被证明是牙周炎和口腔带菌的理想生物标志物。
{"title":"Evaluation of circulating IgG antibodies against Porphyromonas gingivalis or its gingipains as serological markers of periodontitis and carriage of the bacterium.","authors":"Laura Massarenti, Claus Henrik Nielsen, Anne Katrine Danielsen, Peter Østrup Jensen, Christian Enevold, Christian Damgaard","doi":"10.1002/JPER.23-0766","DOIUrl":"https://doi.org/10.1002/JPER.23-0766","url":null,"abstract":"<p><strong>Background: </strong>Increasing evidence indicates that periodontitis contributes to systemic low-grade inflammation. Porphyromonas gingivalis is strongly associated with periodontitis, and antibodies against the bacterium may be used as a serological proxy to account for periodontal status, when studying diseases associated with periodontitis. The aim of the present study is to identify an easily accessible and reliable serological biomarker for determination of periodontal status and oral carriage of the bacterium.</p><p><strong>Methods: </strong>Saliva and serum samples were collected from periodontally healthy controls (n = 27), and patients with periodontitis stage II (n = 12) or stages III or IV (n = 44). Serum levels of immunoglobulin G (IgG) antibodies against intact and fragmented P. gingivalis, recombinant gingipains (RgpA and RgpB), and the bacteria Escherichia coli and Capnocytophaga ochracea as controls were quantified with a multiplex bead-based assay. P. gingivalis was identified in saliva using quantitative polymerase chain reaction (qPCR).</p><p><strong>Results: </strong>Serum IgG antibodies against P. gingivalis whole bacteria were good indicators of periodontitis (area under the curve [AUC]: 0.75, 95% confidence interval [CI]: 0.64-0.85). The same was observed for levels of antibodies against P. gingivalis fragments (AUC: 0.78, 95% CI: 0.68-0.88). Likewise, levels of antibodies against P. gingivalis whole bacteria or P. gingivalis fragments were good indicators of oral carriage of P. gingivalis (AUC: 0.92, 95% CI: 0.86-0.98 and AUC: 0.96, 95% CI: 0.92-1, respectively). Conversely, antibodies against recombinant RgpA and RgpB were not good indicators of periodontitis or oral carriage of the bacterium. None of the antibody levels differed significantly between stage II and stage III or IV periodontitis.</p><p><strong>Conclusion: </strong>Serum IgG antibody levels against heat-inactivated whole P. gingivalis proved to be the preferable biomarker for periodontitis and oral carriage of the bacterium.</p>","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anna Dai, Hao-Yu Li, Sen Kang, Renjie Lin, Jia-Ping Huang, Feifei Mao, Pei-Hui Ding
Background: To date, the clinical evidence regarding the effectiveness of alveolar ridge preservation (ARP) in restricting alveolar bone height and width change after extraction at periodontally compromised molar extraction sockets still remains controversial. This retrospective cohort study aims to evaluate the effect of ARP in molars extracted for periodontal reasons.
Methods: Retrospective data were collected from patient electronic records from January 2019 to December 2023. Patients with Stage III/IV periodontitis who underwent extraction of molars for periodontal reasons were screened for eligibility. The outcomes included the horizontal and vertical dimensions of alveolar bone. The need for additional augmentation procedure during implantation was also evaluated. A linear regression model was used to adjust for known confounders.
Results: A total of 80 sockets were included in this study, of which 27 sockets received ARP therapy after extraction while 53 sockets experienced natural healing (NH). ARP resulted in significantly less bone height change in the periodontally compromised molar sites compared to the NH group (p < 0.001). In sockets displaying a height disparity of >2 mm between the buccal and palatal/lingual walls, the ARP group exhibited advantageous outcomes in terms of ridge width change, surpassing the NH group (p = 0.004). Moreover, the percentage for additional augmentation was significantly reduced in the ARP compared to the NH group (p = 0.006). Age, sex, smoking, jaw, location, and buccal wall thickness did not show any significant effect on bone height change.
Conclusion: ARP had benefits on limiting ridge resorption subsequent to molar extraction for periodontal reasons.
{"title":"Effect of alveolar ridge preservation at periodontally compromised molar extraction sockets: A retrospective cohort study.","authors":"Anna Dai, Hao-Yu Li, Sen Kang, Renjie Lin, Jia-Ping Huang, Feifei Mao, Pei-Hui Ding","doi":"10.1002/JPER.24-0064","DOIUrl":"https://doi.org/10.1002/JPER.24-0064","url":null,"abstract":"<p><strong>Background: </strong>To date, the clinical evidence regarding the effectiveness of alveolar ridge preservation (ARP) in restricting alveolar bone height and width change after extraction at periodontally compromised molar extraction sockets still remains controversial. This retrospective cohort study aims to evaluate the effect of ARP in molars extracted for periodontal reasons.</p><p><strong>Methods: </strong>Retrospective data were collected from patient electronic records from January 2019 to December 2023. Patients with Stage III/IV periodontitis who underwent extraction of molars for periodontal reasons were screened for eligibility. The outcomes included the horizontal and vertical dimensions of alveolar bone. The need for additional augmentation procedure during implantation was also evaluated. A linear regression model was used to adjust for known confounders.</p><p><strong>Results: </strong>A total of 80 sockets were included in this study, of which 27 sockets received ARP therapy after extraction while 53 sockets experienced natural healing (NH). ARP resulted in significantly less bone height change in the periodontally compromised molar sites compared to the NH group (p < 0.001). In sockets displaying a height disparity of >2 mm between the buccal and palatal/lingual walls, the ARP group exhibited advantageous outcomes in terms of ridge width change, surpassing the NH group (p = 0.004). Moreover, the percentage for additional augmentation was significantly reduced in the ARP compared to the NH group (p = 0.006). Age, sex, smoking, jaw, location, and buccal wall thickness did not show any significant effect on bone height change.</p><p><strong>Conclusion: </strong>ARP had benefits on limiting ridge resorption subsequent to molar extraction for periodontal reasons.</p>","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}