Journal of photochemistry and photobiology. B, Biology最新文献_第5页

Femtosecond laser and bee venom as promising anti-arthritic treatments: Modulation of JAK/STAT and PI3K/AKT/mTOR signaling pathways in vivo 飞秒激光和蜂毒作为有前景的抗关节炎治疗：体内JAK/STAT和PI3K/AKT/mTOR信号通路的调节

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-12-05 DOI: 10.1016/j.jphotobiol.2025.113334

Jihad M. El-Sayed , Sally M. Khadrawy , Hanaa M. Mohamed , Magdy Sayed Aly , Abdelwahab Khalil , Dina Sabry , Tarek Mohamed

Rheumatoid arthritis (RA) is a chronic condition characterized by joint degradation and systemic manifestations, which increase the risk of mortality and disability. This study compared the effects of bee venom (BV; subcutaneously administered at 1 mg/kg) and femtosecond laser irradiation (FSL; 830 nm wavelength, 200 mW power, 120 s exposure time, 0.8 cm² beam area with a 0.5 cm radius, 0.25 W/cm² power density, and 30 J/cm² energy dose), either individually or in combination, on arthritic rats. Forty-two adult male Wistar rats were allocated into seven groups. Groups 1–3 served as the negative control, BV, and FSL groups, respectively, while group 4 functioned as the arthritic model group that received 100 μL/rat of complete Freund's adjuvant (CFA) in the right hind paw. Groups 5–7 included arthritic rats treated with BV, FSL, or their combination, respectively. Histological examination of RA development, showing synovitis, cellular infiltration, and cartilage degeneration. Treatment with BV injections and FSL irradiation significantly reduced right hind paw edema, improved histological abnormalities, and reduced serum levels of C-reactive protein (CRP) and rheumatoid factor (RF), alongside decreased tissue expressions of TNF-α, NF-KB, and IL-6 in the affected ankle joints. Moreover, both treatments mitigated oxidative stress, reduced DNA damage, and regulated PI3K/AKT/mTOR and JAK/STAT signaling pathways. Collectively, FSL, either alone or in combination with BV, demonstrated a superior capacity for cartilage regeneration and tissue repair. This highlights BV and FSL as a promising RA therapy, addressing underlying mechanisms beyond symptom relief.

类风湿性关节炎（RA）是一种以关节退化和全身表现为特征的慢性疾病，它增加了死亡和残疾的风险。本研究比较了蜂毒（BV；皮下注射剂量为1mg /kg）和飞秒激光照射（FSL； 830nm波长，200mw功率，120s照射时间，0.8 cm2光束面积，0.5 cm半径，0.25 W/cm2功率密度，30 J/cm2能量剂量）单独或联合对关节炎大鼠的影响。将42只成年雄性Wistar大鼠分为7组。1 ~ 3组分别作为阴性对照组、BV组和FSL组，4组作为关节炎模型组，右后爪给予100 μL/大鼠完全弗氏佐剂（CFA）。5-7组分别用BV、FSL或两者联合治疗关节炎大鼠。类风湿关节炎的组织学检查显示滑膜炎、细胞浸润和软骨变性。BV注射和FSL照射治疗可显著减轻右后足水肿，改善组织学异常，降低血清c反应蛋白（CRP）和类风湿因子（RF）水平，同时降低受影响踝关节组织中TNF-α、NF-KB和IL-6的表达。此外，这两种处理都减轻了氧化应激，减少了DNA损伤，并调节了PI3K/AKT/mTOR和JAK/STAT信号通路。总的来说，无论是单独使用FSL还是与BV联合使用FSL，都显示出更强的软骨再生和组织修复能力。这突出了BV和FSL作为一种有希望的RA治疗方法，解决了症状缓解之外的潜在机制。

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引用次数: 0

Effects of red light-emitting diode therapy in imiquimod-induced psoriasis in mice 红色发光二极管治疗吡喹莫德诱导的小鼠银屑病的效果。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-12-05 DOI: 10.1016/j.jphotobiol.2025.113333

Mab P. Corrêa , Rebeca D. Correia-Silva , Diego D. Santos , Nathália Rodrigues-Silva , Pâmela P. Borges , Silvana Sandri , Karin V. Greco , Cristiane D. Gil

Growing evidence supports the therapeutic potential of phototherapies, particularly light-emitting diodes (LEDs), in modulating inflammation and restoring tissue homeostasis. Here, we evaluated the effects of red LED irradiation (660 nm) in a murine model of psoriasis induced by imiquimod (IMQ). C57BL/6 mice were treated with IMQ for 12 consecutive days and exposed to LED on alternate days from day 4, totalling five sessions. Macroscopic evaluation showed a significant reduction in skin-fold thickness and Psoriasis Area and Severity Index (PASI) scores in LED-treated mice. Histological analyses confirmed that IMQ induced epidermal thickening, erythema, mast cell infiltration, and collagen alterations. LED exposure attenuated epidermal changes (p < 0.0001, IMQ + LED versus IMQ), reduced mast cell numbers (p < 0.05, IMQ + LED versus IMQ), and modulated collagen fiber distribution, although it did not reverse dermal thickening. At the systemic level, IMQ increased spleen weight and white pulp expansion, effects not prevented by LED. In skin samples, IMQ markedly elevated interleukin (IL)-1β and tumor necrosis factor (TNF)-α levels, which were significantly reduced by LED treatment (p < 0.05). In plasma, IL-22 was elevated in IMQ-treated animals but decreased in the IMQ + LED group (p < 0.05), while no significant changes were detected for IL-1β, IL-17A, IL-23, TNF-α, or macrophage inflammatory protein (MIP)-3α. Together, these results suggest that red LED phototherapy reduces clinical severity and partially modulates both local and systemic inflammation in IMQ-induced psoriasis, supporting its potential as a complementary therapeutic approach.

越来越多的证据支持光疗法，特别是发光二极管（led）在调节炎症和恢复组织稳态方面的治疗潜力。在这里，我们评估了红色LED照射（660 nm）对咪喹莫特（IMQ）诱导的银屑病小鼠模型的影响。C57BL/6小鼠IMQ连续治疗12天，从第4天开始隔天暴露于LED，共5个疗程。宏观评价显示，led治疗小鼠的皮肤褶皱厚度和银屑病面积和严重程度指数（PASI）评分显著降低。组织学分析证实IMQ诱导表皮增厚、红斑、肥大细胞浸润和胶原蛋白改变。LED照射可减弱表皮变化(p

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引用次数: 0

Scalable fabrication of polymeric dissolving microneedles for optimized ALA delivery in photodynamic therapy 聚合物溶解微针的可扩展制造，用于优化光动力治疗中ALA的输送

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-12-04 DOI: 10.1016/j.jphotobiol.2025.113319

Dianeth Sara Lima Bejar , Michelle Barreto Requena , Mirian Denise Stringasci , Marlon Rodrigues Garcia , Erika Toneth Ponce Ayala , Juliana Cristina Barreiro , Sebastião Pratavieira , Vanderlei Salvador Bagnato

Topical photodynamic therapy (PDT) is a minimally invasive, clinically approved treatment for non-melanoma skin cancer that relies on the conversion of photosensitizer (PS) precursors such as 5-aminolevulinic acid (ALA) into protoporphyrin IX (PpIX), followed by light activation. However, the low skin penetration of topically applied ALA cream remains a major limitation, restricting effective PpIX accumulation in deeper tumor layers. To address this challenge, we produced dissolving microneedles (DMN) as an alternative intradermal delivery platform. Two mold types were evaluated for DMN fabrication, one with a slight edge (DMNe) and another without edges (DMNf), both maintaining a conical tip geometry. DMN were prepared with a formulation containing initially 10% ALA and 20% Gantrez® AN-139 polymer in water, produced in a few steps, and characterized. In vitro insertion studies demonstrated consistent penetration depths of approximately

250 μ m

with minimal tip deformation. DMNf showed a better penetration efficiency than the DMNe and cream groups, and mass spectrometry confirmed uniform ALA distribution. In vitro assays in darkness confirmed the formulation’s biocompatibility with tumor cells. In a murine xenograft model of nodular epidermoid carcinoma, DMN-mediated ALA delivery generated up to twice the amount of PpIX in deeper tumor regions and also caused greater PDT damage compared to cream application. These findings highlight DMN as a promising approach to enhance PDT efficacy, especially for thicker or nodular skin lesions, by enabling superior and uniform intradermal drug delivery.

局部光动力疗法（PDT）是一种微创、临床批准的非黑色素瘤皮肤癌治疗方法，它依赖于光敏剂（PS）前体如5-氨基乙酰丙酸（ALA）转化为原卟啉IX (PpIX)，然后进行光激活。然而，局部应用ALA霜的皮肤渗透性低仍然是一个主要限制，限制了PpIX在更深肿瘤层的有效积累。为了解决这一挑战，我们生产了溶解微针（DMN）作为另一种皮内给药平台。评估了DMN制造的两种模具类型，一种带有轻微边缘（DMNe），另一种没有边缘（DMNf），两者都保持锥形尖端几何形状。DMN的配方最初含有10% ALA和20% Gantrez®AN-139聚合物在水中，通过几个步骤生产，并表征。体外插入研究表明，穿透深度约为250μm，尖端变形最小。DMNf的穿透效率优于DMNe组和乳膏组，质谱分析证实ALA分布均匀。体外暗室实验证实了该制剂与肿瘤细胞的生物相容性。在小鼠结节性表皮样癌异种移植模型中，dmn介导的ALA递送在更深的肿瘤区域产生的PpIX量高达两倍，并且与乳霜相比，也造成了更大的PDT损伤。这些发现突出了DMN作为一种有希望的方法来提高PDT的疗效，特别是对于较厚或结节性皮肤病变，通过实现优越和均匀的皮内给药。

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引用次数: 0

Photobiomodulation-driven cardiomyogenic differentiation and endothelial vascularization: A dual approach with Aloe Vera 光生物调节驱动的心肌分化和内皮血管化：芦荟的双重途径。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-12-02 DOI: 10.1016/j.jphotobiol.2025.113331

Tuğçe Gültan, Menemşe Gümüşderelioğlu

Cardiomyogenic differentiation plays a critical role in cardiac tissue engineering. It enables the transformation of precursor cells into functional cardiomyocytes capable of contraction and pumping. This study investigates the synergistic effects of Aloe vera (AV) and photobiomodulation (PBM) on cardiomyogenic differentiation in rat cardiomyoblast cell line derived from embryonic ventricular heart tissue (H9C2). It also examines the vascularization potential of primary rat cardiac microvascular endothelial cells (CMECs). Given their natural interaction in cardiac tissue, H9C2 and CMECs were co-cultured at a 6:1 ratio. Cytotoxicity assays established 10 mg/mL AV as a safe concentration, with cell viabilities exceeding 80 % across both monocultures and co-cultures. In the culture, PBM was applied every other day for 10 days, using a polychromatic light source (600–1200 nm) placed 20 cm away from the cells, at an irradiance of 0.04 W/cm² for 3 min per day. Neither AV nor PBM negatively affected cell viability. Fluorescence imaging indicated enhanced alignment and nuclear fusion in H9C2 cells, suggesting cardiomyogenic differentiation, particularly under combined AV–PBM treatment. Moreover, both AV and AV–PBM treatments significantly improved CMECs vascularization potential by day 14. RT-qPCR revealed a 7-fold and 9-fold increase in FGF2 gene expression following PBM and AV–PBM treatment, respectively. Anti-von Willebrand factor staining and imaging confirmed that CMECs formed lumen-like structures beneath H9C2 cells under all treatment conditions. These results demonstrate that the combined application of AV and PBM effectively promotes cardiomyogenic differentiation in H9C2 cells and enhances CMEC-mediated vascularization. This suggests a promising strategy for optimizing biosignaling and tissue integration in cardiac tissue engineering.

心肌分化在心脏组织工程中起着至关重要的作用。它使前体细胞转化为能够收缩和泵送的功能性心肌细胞。本研究探讨芦荟（AV）和光生物调节（PBM）对大鼠胚胎心室组织（H9C2）成心肌细胞分化的协同作用。它还检测了原代大鼠心脏微血管内皮细胞（CMECs）的血管化潜能。考虑到H9C2和cmec在心脏组织中的天然相互作用，以6:1的比例共培养。细胞毒性试验确定10 mg/mL AV为安全浓度，在单培养和共培养中细胞存活率均超过80%。在培养中，每隔一天使用PBM，持续10天，使用多色光源（600-1200 nm），放置在距离细胞20厘米的地方，辐照度为0.04 W/cm2，每天使用3分钟。AV和PBM均未对细胞活力产生负面影响。荧光成像显示H9C2细胞的排列和核聚变增强，提示心肌分化，特别是在AV-PBM联合治疗下。此外，AV和AV- pbm治疗在第14天显著改善了cmec的血管化潜力。RT-qPCR显示，PBM和AV-PBM治疗后，FGF2基因表达分别增加了7倍和9倍。Anti-von Willebrand factor染色和成像证实，在所有处理条件下，cmec在H9C2细胞下形成管腔样结构。这些结果表明，AV和PBM联合应用可有效促进H9C2细胞的成心分化，增强cmec介导的血管化。这为优化心脏组织工程中的生物信号和组织整合提供了一个有前途的策略。

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引用次数: 0

UVB enhances SLC6A15-mediated phenylalanine transport to promote melanogenesis UVB增强slc6a15介导的苯丙氨酸转运，促进黑色素生成

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-30 DOI: 10.1016/j.jphotobiol.2025.113329

Shu Zhou , Yujie Ouyang , Yibo Hu , Xixia Dai , Ling Jiang , Chuhan Fu , Yaqing Wen , Jiangfeng Huang , Keyi Zhang , Jing Chen , Qinghai Zeng

Background

Abnormal melanogenesis is a fundamental biological process underlying pigmentary disorders. While several solute carrier (SLC) transporters have been implicated in its regulation, the functions of many SLCs members remain poorly characterized.

Methods

We delineated transcriptional features of melanocytes with high melanogenic activity using single-cell and bulk RNA sequencing. Key SLC genes associated with melanogenesis were identified and prioritized using machine learning and Mendelian randomization analyses, followed by in vitro functional validation.

Results

Integrated multi-omics analysis identified SLC6A15 as a potential regulator of melanogenesis. SLC6A15 knockdown in MNT1 cells and primary melanocytes reduced melanin synthesis and downregulated key melanogenesis-related genes (e.g., MITF, TYR, DCT). Fontana–Masson staining and tyrosinase activity assays confirmed a marked decrease in melanin deposition and TYR activity in SLC6A15-depleted cells. UVB exposure upregulated SLC6A15, whereas SLC6A15 silencing abrogated the UVB-induced increase in pigmentation. Mechanistically, SLC6A15 knockdown reduced intracellular phenylalanine levels, indicating a role for SLC6A15-mediated phenylalanine transport in melanogenesis. Mendelian randomization further suggested that genetically higher phenylalanine levels are associated with an increased risk of melasma (OR = 6.02).

Conclusions

Our findings identify SLC6A15 as a potential key regulator of melanogenesis through the transport of phenylalanine.

背景：异常黑色素形成是色素紊乱的一个基本生物学过程。虽然一些溶质载体（SLC）转运体参与了其调控，但许多SLC成员的功能仍然缺乏表征。方法利用单细胞和大体积RNA测序技术，对具有高黑色素生成活性的黑素细胞的转录特征进行了描述。通过机器学习和孟德尔随机化分析，确定了与黑色素形成相关的关键SLC基因，并对其进行了优先排序，随后进行了体外功能验证。结果综合多组学分析发现SLC6A15是黑色素形成的潜在调节因子。SLC6A15在MNT1细胞和原代黑素细胞中的敲低降低了黑色素合成，下调了关键的黑素形成相关基因（如MITF、TYR、DCT）。Fontana-Masson染色和酪氨酸酶活性测定证实，slc6a15缺失的细胞中黑色素沉积和TYR活性显著降低。UVB暴露上调了SLC6A15，而SLC6A15沉默消除了UVB诱导的色素沉着增加。在机制上，SLC6A15敲除降低了细胞内苯丙氨酸水平，表明SLC6A15介导的苯丙氨酸运输在黑色素形成中的作用。孟德尔随机化进一步表明，遗传上较高的苯丙氨酸水平与黄褐斑风险增加相关（OR = 6.02）。结论研究结果表明，SLC6A15可能是通过苯丙氨酸转运介导黑色素形成的关键调控因子。

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引用次数: 0

Analysis of ROS dynamics based on dissolved oxygen sensing in upconversion nanoparticle-based photodynamic therapy 上转换纳米粒子光动力治疗中溶解氧传感的ROS动力学分析。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-29 DOI: 10.1016/j.jphotobiol.2025.113328

Esmaeil Heydari , Sepideh Delavari , Motahareh Jafarpour , Kamal Hajisharifi , Mohammad Moeini , Ahmet Erdem , Hossein Zare-Behtash , Mohammadreza Razzaghi , Gongxun Bai

Photodynamic therapy (PDT) is a promising cancer treatment approach that relies on the localized generation of reactive oxygen species (ROS) to eliminate cancer cells. In particular, the nanophotonic approach based on upconversion nanoparticles (UCNPs) offers a key advantage by enabling the use of near-infrared (NIR) light, which enhances light penetration into tissue and expands clinical applicability of PDT. Real-time monitoring of ROS generation and degradation during the PDT process offers distinct advantages over conventional endpoint assays for elucidating PDT mechanisms, optimizing photosensitizer (PS) formulations and refining treatment protocols.

In this study, we not only distinguish and quantify the relative contribution of NaYF₄:Yb³⁺,Tm³⁺ UC nano-antennas, Rose Bengal (RB) PS, NIR activation laser, and culture medium in UCNP-based PDT for the first time via real-time ROS analysis using dissolved oxygen (DO) data which cannot be achieved by endpoint assays but also introduce new and insightful concepts such as medium activation time (FWHM), maximum PL lifetime change (Δτ_max), and time to reach the maximum PL lifetime change ₍τ_max). This is realized by implementation of a 3D-printed optofluidic dissolved oxygen (DO) sensor for indirect analysis of ROS dynamics which infer from changes in the sensor's photoluminescence (PL) lifetime (τ).

Thus, performance and optimum concentrations of NaYF₄:Yb³⁺,Tm³⁺ UCNPs and RB PS are first determined via MTT assays using A375 melanoma cells, and subsequent in-vitro PDT tests using a 980 nm laser. Quantitative analyses show that, UCNPs, RB, and the cell culture medium contribute approximately 25 %, 26 %, and 4 % to the total Δτ respectively. The maximum performance occurs when all components are present and activated, resulting in the highest ROS level with the longest activation time. Interestingly, even laser excitation of the medium alone or UCNPs without PS results in partial ROS generation. These findings provide valuable insights for optimizing UCNP-based PDT drugs for cancer treatment.

光动力疗法（PDT）是一种很有前途的癌症治疗方法，它依赖于局部产生活性氧（ROS）来消除癌细胞。特别是，基于上转换纳米粒子（UCNPs）的纳米光子方法通过使用近红外（NIR）光提供了一个关键优势，它增强了光穿透组织并扩大了PDT的临床适用性。在PDT过程中，实时监测ROS的生成和降解，在阐明PDT机制、优化光敏剂（PS）配方和改进处理方案方面，比传统的终点分析具有明显的优势。在这项研究中，我们不仅通过使用溶解氧（DO）数据进行实时ROS分析，首次区分和量化了基于ucnp的PDT中NaYF4:Yb3+，Tm3+ UC纳米天线，Rose Bengal (RB) PS，近红外激活激光器和培养基的相对贡献，这是终点分析无法实现的，而且还引入了新的和有见解的概念，如介质激活时间（FWHM），最大PL寿命变化（Δτmax）和达到最大PL寿命变化的时间（τmax）。这是通过实现一个3d打印的光流体溶解氧（DO）传感器来间接分析ROS动力学，这是从传感器的光致发光（PL）寿命（τ）的变化中推断出来的。因此，首先使用A375黑色素瘤细胞通过MTT试验确定NaYF4:Yb3+，Tm3+ UCNPs和RB PS的性能和最佳浓度，然后使用980 nm激光进行体外PDT试验。定量分析表明，UCNPs、RB和细胞培养基分别约占总量的25%、26%和4% Δτ。当所有组件都存在并激活时，产生最高的ROS水平，激活时间最长。有趣的是，即使激光激发单独的介质或不含PS的UCNPs也会产生部分ROS。这些发现为优化基于ucnp的PDT药物用于癌症治疗提供了有价值的见解。

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引用次数: 0

Photodynamic ablation of floating lung cancer cells using PVA and TPGS emulsified PLGA nanoparticles loaded with pyropheophorbide-a PVA和TPGS乳化PLGA纳米颗粒负载焦磷-a的光动力消融漂浮肺癌细胞。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-26 DOI: 10.1016/j.jphotobiol.2025.113318

Sasivimon Pramual , Philippe Arnoux , Kriengsak Lirdprapamongkol , Céline Frochot , Valérie Jouan-Hureaux , Muriel Barberi-Heyob , Jisnuson Svasti

Metastasis or the spread of cancer cells to other tissues is a hallmark that leads to the majority of cancer-related deaths worldwide. When metastasizing cancer cells invade into the bloodstream, they become floating cells, also known as circulating tumor cells (CTCs), which can lead to the development of metastasis-associated multidrug resistance in advanced cancer patients. Eradication of CTCs has received much attention as a strategy for preventing metastasis. Photodynamic therapy (PDT) has attracted growing interest as a minimally invasive approach for cancer treatment. Pyropheophorbide-a (PPa) is photosensitizer with advantages of relatively high wavelength absorption and high extinction coefficient; however, it has limited PDT therapeutic benefits due to poor solubility. This work aimed to employ PDT for killing CTCs by utilizing PVA and TPGS coated PLGA nanoparticles, loaded with PPa. The PPa-entrapped PLGA nanoparticles (PPa-NPs) exhibited a spherical morphology under TEM with an average size of 124.9 ± 2.3 nm and a zeta potential value of −32.0 ± 1.4 mV. The PPa-NPs enhanced singlet oxygen generation in water upon light activation. PPa-NPs successfully delivered PPa into A549 floating cells under CTC-mimicking conditions, with 21-fold increase in intracellular PPa accumulation when compared to free PPa treatment. After red light excitation, intracellular ROS level was elevated in PPa-NPs treated floating cells, in a dose-dependent manner, and correlated with photocytotoxic effect of PPa-NPs in the floating cells. Our results demonstrate that PVA and TPGS stabilized PLGA NPs efficiently preserved the photophysical properties of PPa for eradicating CTCs by PDT with red light activation.

癌细胞转移或扩散到其他组织是导致世界上大多数癌症相关死亡的一个标志。当转移性癌细胞侵入血液时，它们成为漂浮细胞，也称为循环肿瘤细胞（ctc），这可能导致晚期癌症患者发生与转移相关的多药耐药。根除ctc作为预防转移的策略已受到广泛关注。光动力疗法（PDT）作为一种微创治疗癌症的方法引起了越来越多的关注。PPa是一种波长吸收高、消光系数高的光敏剂；然而，由于溶解性差，它的PDT治疗效果有限。本工作旨在利用PVA和TPGS包被的PLGA纳米颗粒，负载PPa，利用PDT杀死ctc。在TEM下，PPa-NPs包埋的PLGA纳米颗粒呈球形，平均尺寸为124.9±2.3 nm， zeta电位值为-32.0±1.4 mV。pa - nps在光活化下增强了水中单线态氧的生成。在模拟ctc的条件下，PPa- nps成功地将PPa传递到A549漂浮细胞中，与游离PPa处理相比，细胞内PPa积累增加了21倍。红光激发后，pa - nps处理的漂浮细胞细胞内ROS水平呈剂量依赖性升高，且与pa - nps对漂浮细胞的光毒作用相关。我们的研究结果表明，PVA和TPGS稳定的PLGA NPs有效地保留了PPa的光物理性质，用于红光活化的PDT根除ctc。

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引用次数: 0

Photodamaged PSII does not accumulate in the non-appressed thylakoid membranes in the absence of PSII repair 在没有PSII修复的情况下，光损伤的PSII不会在非贴载的类囊体膜中积累

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-25 DOI: 10.1016/j.jphotobiol.2025.113317

Cleo Bagchus, Lennart A.I. Ramakers, Dana Verhoeven, Herbert van Amerongen, Emilie Wientjes

Photosystem II (PSII) is highly sensitive to light-induced damage. Photoinhibition, the light-dependent inactivation of PSII, is associated with an increase of excitation-energy quenching. Recovery from photoinhibition involves migration of PSII complexes from the appressed to the non-appressed region of the thylakoid membrane, where D1 (the PSII core protein most sensitive to photodamage) is degraded and repair occurs. However, it remains unclear whether damaged PSII core complexes accumulate in the stroma lamellae when repair is blocked.

Here, we combined confocal Fluorescence Lifetime Imaging Microscopy (FLIM) with biochemical fractionation of the thylakoid membrane to investigate the localization of damaged PSII following photoinhibition in the presence of lincomycin, an inhibitor of D1 synthesis. This condition mimics natural stress scenarios such as heat, where D1 synthesis is impaired.

FLIM analysis of structured, intact thylakoid membranes, segmented into grana- and stroma-lamellae-enriched regions, revealed a decrease in PSII fluorescence lifetime upon photoinhibition, consistent with increased excitation-energy quenching. Surprisingly, no significant difference in fluorescence lifetime components was observed between membrane domains, suggesting that damaged, quenched PSII does not accumulate in the stroma lamellae under these conditions. Western blot analysis of biochemically isolated membrane fractions confirmed a uniform decrease in D1 levels across grana and stroma lamellae upon photoinhibition.

Our results indicate that when D1 synthesis is blocked, the relocation and degradation of photodamaged PSII proceed efficiently enough to prevent its accumulation in the stroma lamellae. This reveals new aspects of PSII repair and demonstrates the strength of FLIM for spatially resolved analysis of the thylakoid membrane.

光系统II （PSII）对光致损伤高度敏感。光抑制，即PSII的光依赖性失活，与激发能量猝灭的增加有关。光抑制的恢复涉及PSII复合物从类囊体膜的受压区迁移到非受压区，在那里D1（对光损伤最敏感的PSII核心蛋白）被降解并进行修复。然而，当修复受阻时，受损的PSII核心复合体是否在基质层中积累尚不清楚。在这里，我们将共聚焦荧光寿命成像显微镜（FLIM）与类囊体膜的生化分离相结合，研究了在D1合成抑制剂林可霉素存在下光抑制后受损PSII的定位。这种情况模拟了自然应激情景，如高温，D1合成受损。对结构完整的类囊体膜（分为颗粒和基质片层富集区）的FLIM分析显示，光抑制后PSII荧光寿命减少，与激发能猝灭增加一致。令人惊讶的是，在膜结构域之间没有观察到荧光寿命成分的显著差异，这表明在这些条件下，受损、猝灭的PSII不会在基质层中积累。生物化学分离膜组分的Western blot分析证实，在光抑制作用下，D1水平在颗粒和基质薄片上均匀下降。我们的研究结果表明，当D1合成被阻断时，光损伤的PSII的重新定位和降解进行得足够有效，以防止其在基质片层中的积累。这揭示了PSII修复的新方面，并证明了FLIM在类囊体膜空间分辨分析中的优势。

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引用次数: 0

Phenotypic, physicochemical and transcriptomic responses of Camellia sinensis cv. ‘Huangjinya’ to UV-B stress 山茶的表型、理化和转录组反应。‘黄金牙’对UV-B的胁迫

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-23 DOI: 10.1016/j.jphotobiol.2025.113313

Yueyue Tian , Meina Jin , Hanyue Wang , Tebin Zhang , Zhengqun Zhang , Ping Sun

Ultraviolet-B (UV-B) is an important environmental factor that seriously affects the biological process of Camellia sinensis cv. ‘Huangjinya’, which is highly susceptible to light stress. To understand the mechanism adapted to UV-B, we analyzed the phenotypical, physicochemical and transcriptomic responses of ‘Huangjinya’ in response to UV-B stress in this study. Phenotypic analysis revealed that UV-B induced a yellowing of ‘Huangjinya’ leaves, accompanied by a significant reduction in chlorophyll and carotenoid content. Physiological assessments showed a marked decline in photosynthetic capacity, with decreased photosynthetic rate, stomatal conductance, and electron transfer rate, alongside increased non-photochemical quenching. Antioxidant enzyme activities, particularly peroxidase (POD), were significantly reduced, while stress-responsive hormones such as abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), and ethylene (ET) were elevated. Under UV-B exposure, a total of 15,170 differentially expressed genes (DEGs) were identified, with 3565 upregulated and 11,605 downregulated genes. These DEGs were primarily involved in metabolic pathways related to pigment biosynthesis, photosynthesis, antioxidant enzymes, phenylpropanoid biosynthesis, and plant hormone signal transduction. Transcriptomic analysis further indicated downregulation of genes associated with chlorophyll and carotenoid biosynthesis, as well as photosynthesis-related genes, while genes involved in flavonoid biosynthesis and stress hormone pathways were upregulated. Additionally, UV-B exposure led to a decrease in free amino acids, caffeine, and soluble sugar content, but an increase in tea polyphenols. These findings suggest that UV-B enhances the ornamental value of ‘Huangjinya’ leaves but adversely affects photosynthetic efficiency and the accumulation of key quality components in tea leaves. The study provides a comprehensive understanding of the molecular and physiological mechanisms underlying response to UV-B stress of ‘Huangjinya’, highlighting the interplay between gene expression and physiological changes in UV-B adaptation.

紫外线b （UV-B）是严重影响茶树生物过程的重要环境因子。“黄金牙”，它对光的压力非常敏感。为了解黄金芽对UV-B胁迫的适应机制，本研究分析了黄金芽对UV-B胁迫的表型、理化和转录组反应。表型分析显示，UV-B诱导“黄金牙”叶片变黄，叶绿素和类胡萝卜素含量显著降低。生理评估显示，光合能力明显下降，光合速率、气孔导度和电子传递速率下降，同时非光化学猝灭增加。抗氧化酶活性，特别是过氧化物酶（POD）显著降低，而应激反应激素如脱落酸（ABA）、茉莉酸（JA）、水杨酸（SA）和乙烯（ET）升高。在UV-B暴露下，共鉴定出15,170个差异表达基因（deg），其中3565个基因上调，11,605个基因下调。这些deg主要参与色素生物合成、光合作用、抗氧化酶、苯丙素生物合成和植物激素信号转导等代谢途径。转录组学分析进一步表明，与叶绿素和类胡萝卜素生物合成相关的基因以及光合作用相关的基因下调，而与类黄酮生物合成和应激激素途径相关的基因上调。此外，UV-B暴露导致游离氨基酸、咖啡因和可溶性糖含量下降，但茶多酚含量增加。综上所述，UV-B增强了“黄金牙”叶片的观赏价值，但对茶叶的光合效率和关键品质成分的积累产生不利影响。本研究为“黄金芽”对UV-B胁迫反应的分子和生理机制提供了全面的认识，突出了基因表达与UV-B适应生理变化之间的相互作用。

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引用次数: 0

Photodynamic therapy and argon laser treatment of white spot lesions: Effects on enamel remineralization and orthodontic bracket bond strength 光动力疗法和氩激光治疗白斑病变：对牙釉质再矿化和正畸支架结合强度的影响。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-22 DOI: 10.1016/j.jphotobiol.2025.113316

Abdullah A. Alnazeh , Muhammad Abdullah Kamran , Abdulaziz Alshahrani , Orjuwan Mohammed Amer , Raniya Alhmedai Kalaf Anzi

Aim

Effect of different prophylactic regimes, i.e., Fluoride, Methylene blue (MB) activated photodynamic therapy (PDT), Argon laser, and photopolymerized resin infiltration, on the microhardness (MH), calcium/phosphorus (Ca/P) ratio, bracket-enamel interface, and shear bond strength (SBS) of orthodontic adhesive adhered to demineralized enamel.

Method

One hundred twenty-five premolars were obtained and disinfected. The buccal surface was coated with acid-resistant varnish, creating a 4 × 6-mm area uncovered for bracket bonding and MH testing. Artificial enamel demineralization mimicking white spot lesion (WSL) was developed, and specimens were allocated into five groups based on prophylactic regimes (n = 25): Group 1 (No pretreatment), Group 2 (Fluoride), Group 3 (MB-PS+ Diode Activation), Group 4 (Argon laser), and Group 5 (photopolymerized resin infiltration). Five samples from each group underwent MH assessment and EDX evaluation for Ca/P ratio. 15 samples per group were bonded with orthodontic brackets and thermocycled. Enamel orthodontic bracket interface assessment was performed using scanning electron microscope(n = 5). SBS testing was executed using universal testing machine(n = 10). Debonded samples were examined according to Adhesive Remnant Index. ANOVA and post hoc Tukey were utilized for intergroup comparisons (p < 0.05).

Results

Group 5- photopolymerized resin infiltration-treated groups presented the highest MH scores (273.45 ± 35.26) and maximum SBS (9.52 ± 0.92 MP). Whereas Group 3 (MB-PS-Diode Activation) displayed the lowest outcomes of MH (135.31 ± 32.11) and minimum bond integrity (5.23 ± 0.71 MPa). In terms of MH scores and SBS, the Argon laser was comparable to resin infiltration (p > 0.05).

Conclusion

Both modalities, photopolymerized resin infiltration and argon laser, can be recommended as effective prophylactic regimens before bonding orthodontic brackets to demineralized enamel.

目的：氟化物、亚甲基蓝（MB）活化光动力疗法（PDT）、氩气激光、光聚合树脂浸润等不同预防方案对脱矿牙釉质上正畸粘接剂显微硬度（MH）、钙磷比（Ca/P）、托槽-牙釉质界面和剪切强度（SBS）的影响。方法：取125颗前磨牙进行消毒。口腔表面涂上耐酸清漆，形成一个4 × 6毫米的区域，用于支架粘合和MH测试。采用人工牙釉质脱矿模拟白斑病变（WSL），根据预防方案将标本分为5组（n = 25）： 1组（无预处理）、2组（氟化物）、3组（MB-PS+二极管激活）、4组（氩激光）和5组（光聚合树脂浸润）。每组取5份样品进行MH和EDX评价钙磷比。每组15个样品与正畸托槽粘接并进行热循环。采用扫描电镜对牙釉质正畸托架界面进行评估（n = 5）。SBS测试采用通用试验机（n = 10）。根据粘接残余指数对脱粘试样进行检测。结果：第5组-光聚合树脂浸润处理组MH评分最高（273.45±35.26），SBS评分最高（9.52±0.92 MP）。而第3组（MB-PS-Diode Activation）的MH最低（135.31±32.11），键完整性最低（5.23±0.71 MPa）。在MH评分和SBS方面，氩激光与树脂浸润相当（p < 0.05）。结论：光聚合树脂浸润和氩激光两种方法均可作为正畸托槽与脱矿牙釉质粘接前的有效预防方案。

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引用次数: 0