Journal of photochemistry and photobiology. B, Biology最新文献_第6页

Analysis of ROS dynamics based on dissolved oxygen sensing in upconversion nanoparticle-based photodynamic therapy 上转换纳米粒子光动力治疗中溶解氧传感的ROS动力学分析。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-29 DOI: 10.1016/j.jphotobiol.2025.113328

Esmaeil Heydari , Sepideh Delavari , Motahareh Jafarpour , Kamal Hajisharifi , Mohammad Moeini , Ahmet Erdem , Hossein Zare-Behtash , Mohammadreza Razzaghi , Gongxun Bai

Photodynamic therapy (PDT) is a promising cancer treatment approach that relies on the localized generation of reactive oxygen species (ROS) to eliminate cancer cells. In particular, the nanophotonic approach based on upconversion nanoparticles (UCNPs) offers a key advantage by enabling the use of near-infrared (NIR) light, which enhances light penetration into tissue and expands clinical applicability of PDT. Real-time monitoring of ROS generation and degradation during the PDT process offers distinct advantages over conventional endpoint assays for elucidating PDT mechanisms, optimizing photosensitizer (PS) formulations and refining treatment protocols.

In this study, we not only distinguish and quantify the relative contribution of NaYF₄:Yb³⁺,Tm³⁺ UC nano-antennas, Rose Bengal (RB) PS, NIR activation laser, and culture medium in UCNP-based PDT for the first time via real-time ROS analysis using dissolved oxygen (DO) data which cannot be achieved by endpoint assays but also introduce new and insightful concepts such as medium activation time (FWHM), maximum PL lifetime change (Δτ_max), and time to reach the maximum PL lifetime change ₍τ_max). This is realized by implementation of a 3D-printed optofluidic dissolved oxygen (DO) sensor for indirect analysis of ROS dynamics which infer from changes in the sensor's photoluminescence (PL) lifetime (τ).

Thus, performance and optimum concentrations of NaYF₄:Yb³⁺,Tm³⁺ UCNPs and RB PS are first determined via MTT assays using A375 melanoma cells, and subsequent in-vitro PDT tests using a 980 nm laser. Quantitative analyses show that, UCNPs, RB, and the cell culture medium contribute approximately 25 %, 26 %, and 4 % to the total Δτ respectively. The maximum performance occurs when all components are present and activated, resulting in the highest ROS level with the longest activation time. Interestingly, even laser excitation of the medium alone or UCNPs without PS results in partial ROS generation. These findings provide valuable insights for optimizing UCNP-based PDT drugs for cancer treatment.

光动力疗法（PDT）是一种很有前途的癌症治疗方法，它依赖于局部产生活性氧（ROS）来消除癌细胞。特别是，基于上转换纳米粒子（UCNPs）的纳米光子方法通过使用近红外（NIR）光提供了一个关键优势，它增强了光穿透组织并扩大了PDT的临床适用性。在PDT过程中，实时监测ROS的生成和降解，在阐明PDT机制、优化光敏剂（PS）配方和改进处理方案方面，比传统的终点分析具有明显的优势。在这项研究中，我们不仅通过使用溶解氧（DO）数据进行实时ROS分析，首次区分和量化了基于ucnp的PDT中NaYF4:Yb3+，Tm3+ UC纳米天线，Rose Bengal (RB) PS，近红外激活激光器和培养基的相对贡献，这是终点分析无法实现的，而且还引入了新的和有见解的概念，如介质激活时间（FWHM），最大PL寿命变化（Δτmax）和达到最大PL寿命变化的时间（τmax）。这是通过实现一个3d打印的光流体溶解氧（DO）传感器来间接分析ROS动力学，这是从传感器的光致发光（PL）寿命（τ）的变化中推断出来的。因此，首先使用A375黑色素瘤细胞通过MTT试验确定NaYF4:Yb3+，Tm3+ UCNPs和RB PS的性能和最佳浓度，然后使用980 nm激光进行体外PDT试验。定量分析表明，UCNPs、RB和细胞培养基分别约占总量的25%、26%和4% Δτ。当所有组件都存在并激活时，产生最高的ROS水平，激活时间最长。有趣的是，即使激光激发单独的介质或不含PS的UCNPs也会产生部分ROS。这些发现为优化基于ucnp的PDT药物用于癌症治疗提供了有价值的见解。

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引用次数: 0

Photodynamic ablation of floating lung cancer cells using PVA and TPGS emulsified PLGA nanoparticles loaded with pyropheophorbide-a PVA和TPGS乳化PLGA纳米颗粒负载焦磷-a的光动力消融漂浮肺癌细胞。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-26 DOI: 10.1016/j.jphotobiol.2025.113318

Sasivimon Pramual , Philippe Arnoux , Kriengsak Lirdprapamongkol , Céline Frochot , Valérie Jouan-Hureaux , Muriel Barberi-Heyob , Jisnuson Svasti

Metastasis or the spread of cancer cells to other tissues is a hallmark that leads to the majority of cancer-related deaths worldwide. When metastasizing cancer cells invade into the bloodstream, they become floating cells, also known as circulating tumor cells (CTCs), which can lead to the development of metastasis-associated multidrug resistance in advanced cancer patients. Eradication of CTCs has received much attention as a strategy for preventing metastasis. Photodynamic therapy (PDT) has attracted growing interest as a minimally invasive approach for cancer treatment. Pyropheophorbide-a (PPa) is photosensitizer with advantages of relatively high wavelength absorption and high extinction coefficient; however, it has limited PDT therapeutic benefits due to poor solubility. This work aimed to employ PDT for killing CTCs by utilizing PVA and TPGS coated PLGA nanoparticles, loaded with PPa. The PPa-entrapped PLGA nanoparticles (PPa-NPs) exhibited a spherical morphology under TEM with an average size of 124.9 ± 2.3 nm and a zeta potential value of −32.0 ± 1.4 mV. The PPa-NPs enhanced singlet oxygen generation in water upon light activation. PPa-NPs successfully delivered PPa into A549 floating cells under CTC-mimicking conditions, with 21-fold increase in intracellular PPa accumulation when compared to free PPa treatment. After red light excitation, intracellular ROS level was elevated in PPa-NPs treated floating cells, in a dose-dependent manner, and correlated with photocytotoxic effect of PPa-NPs in the floating cells. Our results demonstrate that PVA and TPGS stabilized PLGA NPs efficiently preserved the photophysical properties of PPa for eradicating CTCs by PDT with red light activation.

癌细胞转移或扩散到其他组织是导致世界上大多数癌症相关死亡的一个标志。当转移性癌细胞侵入血液时，它们成为漂浮细胞，也称为循环肿瘤细胞（ctc），这可能导致晚期癌症患者发生与转移相关的多药耐药。根除ctc作为预防转移的策略已受到广泛关注。光动力疗法（PDT）作为一种微创治疗癌症的方法引起了越来越多的关注。PPa是一种波长吸收高、消光系数高的光敏剂；然而，由于溶解性差，它的PDT治疗效果有限。本工作旨在利用PVA和TPGS包被的PLGA纳米颗粒，负载PPa，利用PDT杀死ctc。在TEM下，PPa-NPs包埋的PLGA纳米颗粒呈球形，平均尺寸为124.9±2.3 nm， zeta电位值为-32.0±1.4 mV。pa - nps在光活化下增强了水中单线态氧的生成。在模拟ctc的条件下，PPa- nps成功地将PPa传递到A549漂浮细胞中，与游离PPa处理相比，细胞内PPa积累增加了21倍。红光激发后，pa - nps处理的漂浮细胞细胞内ROS水平呈剂量依赖性升高，且与pa - nps对漂浮细胞的光毒作用相关。我们的研究结果表明，PVA和TPGS稳定的PLGA NPs有效地保留了PPa的光物理性质，用于红光活化的PDT根除ctc。

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引用次数: 0

Photodamaged PSII does not accumulate in the non-appressed thylakoid membranes in the absence of PSII repair 在没有PSII修复的情况下，光损伤的PSII不会在非贴载的类囊体膜中积累

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-25 DOI: 10.1016/j.jphotobiol.2025.113317

Cleo Bagchus, Lennart A.I. Ramakers, Dana Verhoeven, Herbert van Amerongen, Emilie Wientjes

Photosystem II (PSII) is highly sensitive to light-induced damage. Photoinhibition, the light-dependent inactivation of PSII, is associated with an increase of excitation-energy quenching. Recovery from photoinhibition involves migration of PSII complexes from the appressed to the non-appressed region of the thylakoid membrane, where D1 (the PSII core protein most sensitive to photodamage) is degraded and repair occurs. However, it remains unclear whether damaged PSII core complexes accumulate in the stroma lamellae when repair is blocked.

Here, we combined confocal Fluorescence Lifetime Imaging Microscopy (FLIM) with biochemical fractionation of the thylakoid membrane to investigate the localization of damaged PSII following photoinhibition in the presence of lincomycin, an inhibitor of D1 synthesis. This condition mimics natural stress scenarios such as heat, where D1 synthesis is impaired.

FLIM analysis of structured, intact thylakoid membranes, segmented into grana- and stroma-lamellae-enriched regions, revealed a decrease in PSII fluorescence lifetime upon photoinhibition, consistent with increased excitation-energy quenching. Surprisingly, no significant difference in fluorescence lifetime components was observed between membrane domains, suggesting that damaged, quenched PSII does not accumulate in the stroma lamellae under these conditions. Western blot analysis of biochemically isolated membrane fractions confirmed a uniform decrease in D1 levels across grana and stroma lamellae upon photoinhibition.

Our results indicate that when D1 synthesis is blocked, the relocation and degradation of photodamaged PSII proceed efficiently enough to prevent its accumulation in the stroma lamellae. This reveals new aspects of PSII repair and demonstrates the strength of FLIM for spatially resolved analysis of the thylakoid membrane.

光系统II （PSII）对光致损伤高度敏感。光抑制，即PSII的光依赖性失活，与激发能量猝灭的增加有关。光抑制的恢复涉及PSII复合物从类囊体膜的受压区迁移到非受压区，在那里D1（对光损伤最敏感的PSII核心蛋白）被降解并进行修复。然而，当修复受阻时，受损的PSII核心复合体是否在基质层中积累尚不清楚。在这里，我们将共聚焦荧光寿命成像显微镜（FLIM）与类囊体膜的生化分离相结合，研究了在D1合成抑制剂林可霉素存在下光抑制后受损PSII的定位。这种情况模拟了自然应激情景，如高温，D1合成受损。对结构完整的类囊体膜（分为颗粒和基质片层富集区）的FLIM分析显示，光抑制后PSII荧光寿命减少，与激发能猝灭增加一致。令人惊讶的是，在膜结构域之间没有观察到荧光寿命成分的显著差异，这表明在这些条件下，受损、猝灭的PSII不会在基质层中积累。生物化学分离膜组分的Western blot分析证实，在光抑制作用下，D1水平在颗粒和基质薄片上均匀下降。我们的研究结果表明，当D1合成被阻断时，光损伤的PSII的重新定位和降解进行得足够有效，以防止其在基质片层中的积累。这揭示了PSII修复的新方面，并证明了FLIM在类囊体膜空间分辨分析中的优势。

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引用次数: 0

Phenotypic, physicochemical and transcriptomic responses of Camellia sinensis cv. ‘Huangjinya’ to UV-B stress 山茶的表型、理化和转录组反应。‘黄金牙’对UV-B的胁迫

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-23 DOI: 10.1016/j.jphotobiol.2025.113313

Yueyue Tian , Meina Jin , Hanyue Wang , Tebin Zhang , Zhengqun Zhang , Ping Sun

Ultraviolet-B (UV-B) is an important environmental factor that seriously affects the biological process of Camellia sinensis cv. ‘Huangjinya’, which is highly susceptible to light stress. To understand the mechanism adapted to UV-B, we analyzed the phenotypical, physicochemical and transcriptomic responses of ‘Huangjinya’ in response to UV-B stress in this study. Phenotypic analysis revealed that UV-B induced a yellowing of ‘Huangjinya’ leaves, accompanied by a significant reduction in chlorophyll and carotenoid content. Physiological assessments showed a marked decline in photosynthetic capacity, with decreased photosynthetic rate, stomatal conductance, and electron transfer rate, alongside increased non-photochemical quenching. Antioxidant enzyme activities, particularly peroxidase (POD), were significantly reduced, while stress-responsive hormones such as abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), and ethylene (ET) were elevated. Under UV-B exposure, a total of 15,170 differentially expressed genes (DEGs) were identified, with 3565 upregulated and 11,605 downregulated genes. These DEGs were primarily involved in metabolic pathways related to pigment biosynthesis, photosynthesis, antioxidant enzymes, phenylpropanoid biosynthesis, and plant hormone signal transduction. Transcriptomic analysis further indicated downregulation of genes associated with chlorophyll and carotenoid biosynthesis, as well as photosynthesis-related genes, while genes involved in flavonoid biosynthesis and stress hormone pathways were upregulated. Additionally, UV-B exposure led to a decrease in free amino acids, caffeine, and soluble sugar content, but an increase in tea polyphenols. These findings suggest that UV-B enhances the ornamental value of ‘Huangjinya’ leaves but adversely affects photosynthetic efficiency and the accumulation of key quality components in tea leaves. The study provides a comprehensive understanding of the molecular and physiological mechanisms underlying response to UV-B stress of ‘Huangjinya’, highlighting the interplay between gene expression and physiological changes in UV-B adaptation.

紫外线b （UV-B）是严重影响茶树生物过程的重要环境因子。“黄金牙”，它对光的压力非常敏感。为了解黄金芽对UV-B胁迫的适应机制，本研究分析了黄金芽对UV-B胁迫的表型、理化和转录组反应。表型分析显示，UV-B诱导“黄金牙”叶片变黄，叶绿素和类胡萝卜素含量显著降低。生理评估显示，光合能力明显下降，光合速率、气孔导度和电子传递速率下降，同时非光化学猝灭增加。抗氧化酶活性，特别是过氧化物酶（POD）显著降低，而应激反应激素如脱落酸（ABA）、茉莉酸（JA）、水杨酸（SA）和乙烯（ET）升高。在UV-B暴露下，共鉴定出15,170个差异表达基因（deg），其中3565个基因上调，11,605个基因下调。这些deg主要参与色素生物合成、光合作用、抗氧化酶、苯丙素生物合成和植物激素信号转导等代谢途径。转录组学分析进一步表明，与叶绿素和类胡萝卜素生物合成相关的基因以及光合作用相关的基因下调，而与类黄酮生物合成和应激激素途径相关的基因上调。此外，UV-B暴露导致游离氨基酸、咖啡因和可溶性糖含量下降，但茶多酚含量增加。综上所述，UV-B增强了“黄金牙”叶片的观赏价值，但对茶叶的光合效率和关键品质成分的积累产生不利影响。本研究为“黄金芽”对UV-B胁迫反应的分子和生理机制提供了全面的认识，突出了基因表达与UV-B适应生理变化之间的相互作用。

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引用次数: 0

Photodynamic therapy and argon laser treatment of white spot lesions: Effects on enamel remineralization and orthodontic bracket bond strength 光动力疗法和氩激光治疗白斑病变：对牙釉质再矿化和正畸支架结合强度的影响。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-22 DOI: 10.1016/j.jphotobiol.2025.113316

Abdullah A. Alnazeh , Muhammad Abdullah Kamran , Abdulaziz Alshahrani , Orjuwan Mohammed Amer , Raniya Alhmedai Kalaf Anzi

Aim

Effect of different prophylactic regimes, i.e., Fluoride, Methylene blue (MB) activated photodynamic therapy (PDT), Argon laser, and photopolymerized resin infiltration, on the microhardness (MH), calcium/phosphorus (Ca/P) ratio, bracket-enamel interface, and shear bond strength (SBS) of orthodontic adhesive adhered to demineralized enamel.

Method

One hundred twenty-five premolars were obtained and disinfected. The buccal surface was coated with acid-resistant varnish, creating a 4 × 6-mm area uncovered for bracket bonding and MH testing. Artificial enamel demineralization mimicking white spot lesion (WSL) was developed, and specimens were allocated into five groups based on prophylactic regimes (n = 25): Group 1 (No pretreatment), Group 2 (Fluoride), Group 3 (MB-PS+ Diode Activation), Group 4 (Argon laser), and Group 5 (photopolymerized resin infiltration). Five samples from each group underwent MH assessment and EDX evaluation for Ca/P ratio. 15 samples per group were bonded with orthodontic brackets and thermocycled. Enamel orthodontic bracket interface assessment was performed using scanning electron microscope(n = 5). SBS testing was executed using universal testing machine(n = 10). Debonded samples were examined according to Adhesive Remnant Index. ANOVA and post hoc Tukey were utilized for intergroup comparisons (p < 0.05).

Results

Group 5- photopolymerized resin infiltration-treated groups presented the highest MH scores (273.45 ± 35.26) and maximum SBS (9.52 ± 0.92 MP). Whereas Group 3 (MB-PS-Diode Activation) displayed the lowest outcomes of MH (135.31 ± 32.11) and minimum bond integrity (5.23 ± 0.71 MPa). In terms of MH scores and SBS, the Argon laser was comparable to resin infiltration (p > 0.05).

Conclusion

Both modalities, photopolymerized resin infiltration and argon laser, can be recommended as effective prophylactic regimens before bonding orthodontic brackets to demineralized enamel.

目的：氟化物、亚甲基蓝（MB）活化光动力疗法（PDT）、氩气激光、光聚合树脂浸润等不同预防方案对脱矿牙釉质上正畸粘接剂显微硬度（MH）、钙磷比（Ca/P）、托槽-牙釉质界面和剪切强度（SBS）的影响。方法：取125颗前磨牙进行消毒。口腔表面涂上耐酸清漆，形成一个4 × 6毫米的区域，用于支架粘合和MH测试。采用人工牙釉质脱矿模拟白斑病变（WSL），根据预防方案将标本分为5组（n = 25）： 1组（无预处理）、2组（氟化物）、3组（MB-PS+二极管激活）、4组（氩激光）和5组（光聚合树脂浸润）。每组取5份样品进行MH和EDX评价钙磷比。每组15个样品与正畸托槽粘接并进行热循环。采用扫描电镜对牙釉质正畸托架界面进行评估（n = 5）。SBS测试采用通用试验机（n = 10）。根据粘接残余指数对脱粘试样进行检测。结果：第5组-光聚合树脂浸润处理组MH评分最高（273.45±35.26），SBS评分最高（9.52±0.92 MP）。而第3组（MB-PS-Diode Activation）的MH最低（135.31±32.11），键完整性最低（5.23±0.71 MPa）。在MH评分和SBS方面，氩激光与树脂浸润相当（p < 0.05）。结论：光聚合树脂浸润和氩激光两种方法均可作为正畸托槽与脱矿牙釉质粘接前的有效预防方案。

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引用次数: 0

Biomimetic polydopamine-intercalated MgAl-layered double hydroxide for effective skin photoprotection and photodamage recovery 仿生多多巴胺嵌入镁铝层双氢氧化物，有效的皮肤光保护和光损伤恢复

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-22 DOI: 10.1016/j.jphotobiol.2025.113314

Pengqi Zhu , Xichen Sun , Ye Cai , Mingchen Zhao , Ruiping Zhang , Jinghua Sun

Excessive ultraviolet (UV) radiation is harmful to human health, leading to a range of skin issues including photoaging, sunburn, and skin cancer. Using sunscreen can help alleviate or provide temporary protection against the harmful effects of UV radiation. Commercial sunscreens frequently have low effectiveness and raise safety concerns. Therefore, a novel biocompatible polydopamine-intercalated MgAl-layered double hydroxides nanocomposite (PDA-LDH) was synthesized via in situ oxidation of dopamine within the interlayer of LDH at room temperature and without any additives. LDH can serve as an effective base to facilitate the formation of PDA without the need for an additional base, due to the ordered arrangement of basic hydroxyl groups on the surface of the LDH. The intercalation of PDA in the LDH interlayer ensures good biosafety, effective UV shielding, and excellent antioxidative and anti-inflammatory properties of PDA-LDH, making it suitable for skin photoprotection and the repair of photodamaged skin. PDA-LDH is poised to be a promising next-generation biomimetic sunscreen, designed to assist in the photoprotection and repair of photodamaged skin.

过度的紫外线（UV）辐射对人体健康有害，会导致一系列皮肤问题，包括光老化、晒伤和皮肤癌。使用防晒霜可以帮助减轻或提供暂时的保护，防止紫外线辐射的有害影响。商业防晒霜的功效往往很低，还会引发安全问题。因此，在室温下，不添加任何添加剂的情况下，通过在LDH层间原位氧化多巴胺，合成了一种新的生物相容性聚多巴胺-插层mgal层双氢氧化物纳米复合材料（PDA-LDH）。由于LDH表面有序排列的碱性羟基，LDH可以作为一个有效的碱，促进PDA的形成，而不需要额外的碱。PDA嵌入LDH中间层，保证了PDA-LDH良好的生物安全性、有效的紫外线屏蔽、优异的抗氧化和抗炎性能，适用于皮肤光保护和光损伤皮肤的修复。PDA-LDH是一种有前途的下一代仿生防晒霜，旨在帮助光保护和修复光损伤的皮肤。

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引用次数: 0

Carbonic anhydrase modulates photosynthetic responses to UV radiation in diatoms across temperature gradients 碳酸酐酶在温度梯度上调节硅藻对紫外线辐射的光合反应

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-21 DOI: 10.1016/j.jphotobiol.2025.113315

Xiangyu Li , Yuheng An , Juntian Xu , Yaping Wu , John Beardall

Diatoms are major contributors to marine primary production and global carbon cycling, while they face increasing physiological stress from climate change–driven shifts in temperature, light regimes, and carbon availability. Carbonic anhydrase (CA) is a key enzyme in diatom carbon-concentrating mechanisms, catalyzing the reversible conversion of CO₂ and HCO₃⁻ to facilitate carbon fixation. Here, we examined how CA inhibition influences the growth and photosynthetic performance of two morphologically distinct diatoms—Skeletonema costatum and Nitzschia sp. (centric vs. pennate)—under ultraviolet radiation (UVR) across three temperatures (15, 20, and 25 °C). Cultures were exposed to photosynthetically active radiation (PAR) or PAR + UVR (PAB), with or without ethoxyzolamide (EZ), a membrane-permeable CA inhibitor. In S. costatum, EZ completely suppressed growth at all temperatures, indicating a strong dependence on CA-mediated CO₂ supply. Nitzschia sp. maintained growth under EZ at 15 and 20 °C but was more affected at 25 °C, suggesting greater resilience through alternative carbon acquisition pathways. Photophysiological measurements showed that CA inhibition substantially reduced maximum relative electron transport rate (rETR_max) and light saturation point (I_k) in S. costatum, with smaller effects in Nitzschia. Under UVR, effective quantum yield (EQY) declined in both species, but the reduction was amplified by CA inhibition, most severely in S. costatum, where UVR-induced EQY inhibition exceeded 75 % at 25 °C. These results highlight that CA plays a critical role in mitigating UVR stress by sustaining CO₂ availability, and that species-specific traits, including differences in cell geometry, carbon uptake systems, and photoprotective capacity, modulate diatom vulnerability to combined warming and UVR. Such species-specific responses could drive shifts in diatom community composition and alter coastal carbon cycling under future climate scenarios.

硅藻是海洋初级生产和全球碳循环的主要贡献者，同时它们面临着气候变化驱动的温度、光照制度和碳可用性变化带来的越来越大的生理压力。碳酸酐酶（CA）是硅藻碳浓缩机制中的关键酶，催化CO₂和HCO₃−的可逆转化，促进碳固定。在这里，我们研究了在三种温度（15、20和25°C）的紫外线辐射（UVR）下，CA抑制如何影响两种形态不同的硅藻——骨骨藻（skeletonema costatum）和尼氏硅藻（Nitzschia sp.）的生长和光合性能。培养物暴露于光合有效辐射（PAR）或PAR + UVR (PAB)，有或没有乙氧基唑胺（EZ），一种膜渗透性CA抑制剂。在S. costatum中，EZ在所有温度下都完全抑制生长，表明对ca介导的CO₂供应有很强的依赖性。Nitzschia sp.在15和20°C的EZ下保持生长，但在25°C的EZ下受影响更大，表明通过其他碳获取途径具有更强的恢复能力。光生理测量表明，CA抑制显著降低了S. costatum的最大相对电子传递速率（rETRmax）和光饱和点（Ik），对Nitzschia的影响较小。在UVR作用下，两种植物的有效量子产率（EQY）均有所下降，但CA的抑制作用放大了有效量子产率的下降，其中以海参（S. costatum）最为严重，在25°C下UVR诱导的EQY抑制超过75%。这些结果表明，CA通过维持CO 2可用性在缓解UVR胁迫中起着关键作用，并且物种特异性性状，包括细胞几何形状、碳吸收系统和光保护能力的差异，调节了硅藻对变暖和UVR的脆弱性。这种物种特异性反应可能会推动硅藻群落组成的变化，并改变未来气候情景下的沿海碳循环。

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引用次数: 0

Cyclic electron transport pathways around photosystems I and II: Working together 光系统I和II的循环电子传递途径：协同工作。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-21 DOI: 10.1016/j.jphotobiol.2025.113312

Vladimir Lysenko , Ya Guo , Maria Ignatova , Ekaterina Tarik , Veronica Aslanyan , Tatyana Varduny , Anastasia Toptunova , Pavel Plyaka , Evgeniya Kirichenko , Pavel Dmitriev , Vladimir Krasnov

The processes of cyclic electron transport around photosystems I and II (CET-PSI and CET-PSII) do not lead to O₂ evolution and CO₂ assimilation and are referred to as anoxygenic photosynthesis a broad sense, in contrast to specific processes in bacteria, which are commonly referred to as anoxygenic photosynthesis in a narrow sense. CET-PSI has been studied much more extensively than CET-PSII. Attempts to quantify CET-PSI have yielded contradictory results. It is not detected in non-stressed C3-plants using photoacoustic methods but is commonly considered as being observed when using Antimycin А which was had previously been proposed as a CET-PSI inhibitor. However, most researchers ignore recent data showing that Antimycin А primarily inhibits rather CET-PSII then CET-PSI. These facts, along with others, suggest that the contribution of CET-PSI to photosynthesis of non-stressed C3-plants has been overestimated. Our analysis of the data in this field also shows the possibility of underestimating CET-PSII, as well as anoxygenic photosynthesis in total, which is not excluded from being dominated over oxygenic photosynthesis. We point out that CET-PSI and CET-PSII cannot be studied separately. The difficulties in the quantitative evaluation of CET-PSII can be solved using photoacoustic techniques, which are highly promising in studies of anoxygenic photosynthesis.

围绕光系统I和II （CET-PSI和CET-PSII）的循环电子传递过程不导致O2演化和CO2同化，被称为广义的无氧光合作用，而细菌中的特定过程通常被称为狭义的无氧光合作用。CET-PSI的研究范围比CET-PSI广泛得多。量化CET-PSI的尝试产生了相互矛盾的结果。使用光声方法在非胁迫c3植物中无法检测到它，但通常认为在使用antiycin А时可以观察到它，这是之前被提议作为CET-PSI抑制剂的一种。然而，大多数研究人员忽略了最近的数据显示，anti - ycin А主要抑制CET-PSII，而不是CET-PSI。这些事实以及其他事实表明，CET-PSI对非胁迫c3植物光合作用的贡献被高估了。我们对该领域数据的分析也显示了低估CET-PSII的可能性，以及总体上无氧光合作用的可能性，不排除其在有氧光合作用中占主导地位。我们指出CET-PSI和CET-PSII不能分开研究。利用光声技术可以解决CET-PSII定量评价的困难，在无氧光合作用研究中具有很大的应用前景。

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引用次数: 0

A sulfonate-modified cyanine-based photoacoustic probe for selective detection of hydroxyl radicals in diabetic liver injury 一种用于糖尿病肝损伤羟基自由基选择性检测的磺胺基修饰菁基光声探针。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-20 DOI: 10.1016/j.jphotobiol.2025.113311

Dong-Xia Fan , Ren-Wei-Yang Zhang , Jun-Lian Liu , Lu Fan , Hong-Xia Xiang , Zhong-Yuan Cheng , Kai Wang

Hydroxyl radicals (•OH) play a critical role in oxidative stress-related diseases, yet their real-time detection in vivo remains challenging. We developed a hydroxyl radical-responsive photoacoustic probe (OHP) by modifying IR780-SO₃H with enhanced hydrophilicity (logP = −1.051) for improved biodistribution. Structural characterization confirmed the selective reduction of the conjugated system, while in vitro studies demonstrated OHP's selective and linear response to •OH with minimal interference from other ROS/RNS. In diabetic mice, OHP enabled dynamic monitoring of hepatic •OH levels, revealing elevated oxidative stress that was attenuated by metformin treatment. Ex vivo fluorescence imaging and histopathology validated the imaging results, showing strong correlation with disease severity. Biosafety assessments confirmed negligible cytotoxicity in cells and mice. OHP represents a sensitive, selective, and biocompatible tool for non-invasive •OH detection, offering potential for studying oxidative stress and therapeutic interventions.

羟基自由基（•OH）在氧化应激相关疾病中起着至关重要的作用，但它们在体内的实时检测仍然具有挑战性。我们通过修饰IR780-SO3H，增强亲水性（logP = -1.051），开发了羟基自由基响应光声探针（OHP），以改善生物分布。结构表征证实了共轭体系的选择性还原，而体外研究表明OHP对•OH具有选择性和线性响应，并且其他ROS/RNS的干扰最小。在糖尿病小鼠中，OHP能够动态监测肝脏•OH水平，揭示二甲双胍治疗后氧化应激升高。离体荧光成像和组织病理学证实了成像结果，显示与疾病严重程度有很强的相关性。生物安全评估证实，细胞和小鼠的细胞毒性可以忽略不计。OHP是一种灵敏、选择性和生物相容性的非侵入性OH检测工具，为研究氧化应激和治疗干预提供了潜力。

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引用次数: 0

Multifunctional PDA–graphene family nanocomposites for antibacterial and anticancer photothermal therapy 用于抗菌和抗癌光热治疗的多功能pda -石墨烯家族纳米复合材料

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-19 DOI: 10.1016/j.jphotobiol.2025.113310

Sepehr Kamalzadeh , Kowsar Moradi , Amir Keshavarz Afshar, Matin Mahmoudifard

The increasing demand for multifunctional, biocompatible nanomaterials has spurred the exploration of hybrid systems with synergistic antimicrobial, anticancer, antioxidant, and regenerative properties. In this study, polydopamine (PDA)-based nanocomposites incorporating graphene oxide (PDA–GO) and graphene quantum dots (PDA–GQD) were synthesized and systematically characterized for their physicochemical and biological functionalities. The nanocomposites raised temperature > 50 °C within 5 min under 808 nm laser irradiation (1.5 W/cm², 250 μg/mL). Both composites showed antibacterial activity against Escherichia coli (E. coli) (Gram-negative) and Staphylococcus aureus (S. aureus) (Gram-positive), achieving >99 % bacterial eradication under 10 min NIR irradiation at 125 μg/mL, indicating a combined photothermal (PT) and oxidative mechanism. In parallel, in vitro cytotoxicity assays revealed selective toxicity toward MCF-7 cancer cells—reducing viability to 95 % viability of normal L929 fibroblasts. Antioxidant assays confirmed >80 % DPPH radical scavenging at 250 μg/mL, supporting their potential in oxidative stress modulation. Furthermore, scratch wound healing assays demonstrated ∼100 % wound closure within 48 h in NIR-irradiated PDA–GO/PDA–GQD groups. Intracellular H₂O₂ generation reached up to 30.5 μM in MCF-7 cells under laser, enabling dual PT–photodynamic (PD) therapy. Altogether, these findings position PDA–GO and PDA–GQD nanocomposites as versatile platforms for integrated antibacterial, anticancer, and wound-healing therapies, highlighting their promise for future biomedical applications beyond conventional monofunctional approaches.

对多功能、生物相容性纳米材料日益增长的需求刺激了对具有协同抗菌、抗癌、抗氧化和再生特性的混合系统的探索。在本研究中，合成了含有氧化石墨烯（PDA - go）和石墨烯量子点（PDA - gqd）的聚多巴胺（PDA）基纳米复合材料，并对其物理化学和生物功能进行了系统表征。在808 nm激光（1.5 W/cm2, 250 μg/mL）照射下，纳米复合材料在5 min内升温50℃。两种复合材料对大肠杆菌（E. coli）（革兰氏阴性）和金黄色葡萄球菌（S. aureus）（革兰氏阳性）均表现出抗菌活性，在125 μg/mL近红外照射10 min下，细菌根除率达到>； 99%，表明复合材料具有光热（PT）和氧化联合作用机制。同时，体外细胞毒性实验显示MCF-7对MCF-7癌细胞具有选择性毒性——将正常L929成纤维细胞的存活率降低至95%。抗氧化实验证实，250 μg/mL的DPPH自由基清除率为80%，支持其在氧化应激调节中的潜力。此外，划痕伤口愈合试验显示，nir辐照的PDA-GO / PDA-GQD组在48小时内伤口愈合~ 100%。在激光照射下，MCF-7细胞内的H₂O₂生成高达30.5 μM，实现了双pt光动力（PD）治疗。总之，这些发现将PDA-GO和PDA-GQD纳米复合材料定位为综合抗菌、抗癌和伤口愈合治疗的多功能平台，突出了它们在未来生物医学应用方面的前景，超越了传统的单功能方法。

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引用次数: 0