Journal of photochemistry and photobiology. B, Biology最新文献_第6页

Unveiling connectivity and differences of bacterial response mechanisms exposed to multi-radiation through the transcriptomic profiles and physiological characteristics of Mount Everest Sphingomonas radiodurans 珠穆朗玛峰耐辐射鞘单胞菌转录组学和生理特征揭示多重辐射下细菌反应机制的连通性和差异

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-08 DOI: 10.1016/j.jphotobiol.2025.113304

Yang Liu , Lu Zhang , Jinxiu Wang , Pengcheng Zhang , Zhuxiang Zhang , Tuo Chen , Guangxiu Liu , Shichang Kang , Wei Zhang , Gaosen Zhang

Radiation-resistant microorganisms employ complex regulatory networks to safeguard cellular protection and DNA repair upon radiation exposure. And, previous studies have focused on a single type of radiation. However, studies specifically exploring the correlations, including connectivity defined as interlinked regulatory networks or coexisted core defense mechanisms, and differences referred to the radiation-type-specific radiation protection and damage repair, between an organism's resistant radiation types and their underlying resistance mechanisms remain limited. Therefore, we conducted an integrated transcriptomic and physiological analysis of Sphingomonas radiodurans from Mount Everest, investigating its connectivity and differences responding to multi-radiations within UVC, γ-ray, and X-ray radiation. For UVC radiation, extracellular polysaccharides reduced direct cell damage, and the RecF homologous recombination pathway was induced to repair DNA DSBs. In response to γ-ray radiation, EPSs also mitigated cell damage; additionally, γ-ray-induced changes in cell membrane proteins and lipids cooperated with EPS to block radiation penetration, and the RecF pathway was activated for DNA DSBs repair. Regarding X-ray radiation, it similarly induced membrane protein and lipid changes to synergize for radiation blocking, but uniquely activated the RecBCD homologous recombination pathway for DNA DSBs repair. Notably, the ROS-scavenging system served as the common connectivity across all three radiation types, mitigating oxidative stress from radiation-induced ROS accumulation. Combined with weighted gene co-expression network analysis, a high proportion of novel genes encoding hypothetical proteins were significantly upregulated in response to multi-radiation. Taken together, these results highlight the coordinated protective strategies of strain S9–5 involving both shared and radiation-specific mechanisms, provide new insights into bacterial response mechanisms of radiation resistance evolution in extreme environments, and serve as important references for developing protection agents against multi-radiation damage.

抗辐射微生物采用复杂的调控网络来保护辐射暴露时的细胞保护和DNA修复。而且，之前的研究都集中在单一类型的辐射上。然而，专门探讨相关性的研究，包括相互关联的调节网络或共存的核心防御机制的连通性，以及生物体耐辐射类型及其潜在抗性机制之间辐射类型特异性辐射防护和损伤修复的差异，仍然有限。因此，我们对来自珠穆朗玛峰的耐辐射鞘单胞菌进行了综合转录组学和生理学分析，研究了其在UVC、γ射线和x射线多重辐射下的连通性和差异。对于UVC辐射，胞外多糖可减少细胞的直接损伤，并可诱导RecF同源重组途径修复DNA dsb。在对γ射线辐射的反应中，eps还能减轻细胞损伤；此外，γ射线诱导的细胞膜蛋白和脂质变化与EPS协同阻止辐射穿透，激活RecF途径修复DNA dsb。在x射线辐射方面，它同样诱导膜蛋白和脂质改变协同作用进行辐射阻断，但它独特地激活了DNA dsb修复的RecBCD同源重组途径。值得注意的是，ROS清除系统作为所有三种辐射类型的共同连接，减轻了辐射诱导的ROS积累引起的氧化应激。结合加权基因共表达网络分析，高比例的编码假设蛋白的新基因在多重辐射下显著上调。综上所述，这些结果突出了菌株S9-5在共享机制和辐射特异性机制下的协同保护策略，为研究极端环境下细菌对辐射抗性进化的响应机制提供了新的见解，并为开发针对多重辐射损伤的防护剂提供了重要参考。

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引用次数: 0

Hypericin-mediated photodynamic therapy promotes apoptosis and inhibits fibrosis by inducing HMOX1-mediated ferroptosis in hypertrophic scar fibroblasts 金丝桃素介导的光动力疗法通过诱导hmox1介导的增生性瘢痕成纤维细胞铁下垂促进细胞凋亡和抑制纤维化。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-07 DOI: 10.1016/j.jphotobiol.2025.113303

Xing Li , Ziqing Chen , Mingzhuo Liu, Jingwen An, Jinwei Shang, Gehua Zhu, Huicai Wen , Guanghua Guo

Background

The treatment of hypertrophic scars poses a significant therapeutic challenge due to the limitations of existing options. Given the inadequacy of current regimens, it is imperative to explore new and more effective treatment strategies.

Objective

Here, we investigated the mechanism by which hypericin-mediated photodynamic therapy (HYP-PDT) inhibits hypertrophic scar formation, with a focus on the role of HMOX1 and ferroptosis.

Methods

Using hypertrophic scar fibroblasts (HFs), we determined optimal HYP-PDT conditions and assessed its effects on cell proliferation, migration, apoptosis, and fibrosis. Integrated transcriptomic and proteomic analyses were performed to identify key targets, followed by mechanistic studies on ferroptosis.

Results

HYP-PDT significantly suppressed HF proliferation, migration, and fibrotic protein expression, while promoting apoptosis. Multi-omics analysis identified HMOX1 as a key upregulated target linked to ferroptosis. HYP-PDT increased ROS, lipid peroxidation, and Fe²⁺ levels—effects reversible by ferroptosis inhibitors. HMOX1 overexpression enhanced HYP-PDT-induced ferroptosis and apoptosis, but alone did not trigger these processes.

Conclusion

This study demonstrates that HYP-PDT induces ferroptosis via HMOX1 upregulation, providing a new mechanistic basis and potential therapeutic target for hypertrophic scar management.

背景：由于现有选择的局限性，增生性疤痕的治疗提出了一个重大的治疗挑战。鉴于目前治疗方案的不足，探索新的和更有效的治疗策略势在必行。目的：在这里，我们研究了金丝桃素介导的光动力疗法（hypp - pdt）抑制增生性瘢痕形成的机制，重点研究了HMOX1和铁下垂的作用。方法：利用增生性瘢痕成纤维细胞（HFs），我们确定了最佳的hypp - pdt条件，并评估了其对细胞增殖、迁移、凋亡和纤维化的影响。综合转录组学和蛋白质组学分析确定关键靶点，随后进行铁下垂的机制研究。结果：hypp - pdt显著抑制HF增殖、迁移和纤维化蛋白表达，促进细胞凋亡。多组学分析发现HMOX1是与铁死亡相关的关键上调靶点。hypp - pdt增加ROS、脂质过氧化和Fe2+水平，这种效应被铁下垂抑制剂逆转。HMOX1过表达增强了hypp - pdt诱导的铁下垂和细胞凋亡，但单独表达不触发这些过程。结论：本研究表明hypp - pdt通过上调HMOX1诱导铁下垂，为增生性瘢痕治疗提供了新的机制基础和潜在的治疗靶点。

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引用次数: 0

Phage-conjugated chlorin e6: A strategy overcoming phage resistance in biofilm eradication and wound infection therapy of carbapenem-resistant Acinetobacter baumannii 噬菌体共轭氯e6：耐碳青霉烯鲍曼不动杆菌生物膜根除和伤口感染治疗中克服噬菌体耐药性的策略。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-07 DOI: 10.1016/j.jphotobiol.2025.113302

Jianhui Su , Siyi Chen , Zhishen Huang , Haoxuan He , Huanhuan Zou , Xiaoyi Huang , Yongzhao Xie , Hongxia Zhao , Zhenbo Xu , Tao Lei , Juan Li , Haiyan Zeng

Carbapenem-Resistant Acinetobacter baumannii (CRAB) frequently causes biofilm-related infections that can exhibit extreme resistance to antibiotic therapy. Phage therapy shows promise as an alternative treatment, yet bacteria may develop resistance to it with prolonged use. Phage-photosensitizer combination therapy represents a novel antimicrobial strategy. This study aims to evaluate the efficacy of chlorin e6-functionalized phage in eradicating biofilms and treating CRAB infections, and to assess its sustained after effect following the emergence of phage resistance. The A. baumannii phage (ABP)-chlorin e6 conjugate (ABP-Ce6) was successfully synthesized and characterized. It preserved the phage's absorptive capacity and lytic activity while enhanced reactive oxygen species (ROS) production. Moreover, ABP-Ce6 demonstrated remarkable antibacterial activity comparable to ABP while exceeding that of Ce6, and showed superior performance in both inhibiting biofilm formation and disrupting existing biofilms in CRAB Ab1513. Significantly, although ABP exhibited no efficacy against the phage-resistant CRAB Ab1513-BIM12 due to its inability to achieve irreversible adsorption, the ABP-Ce6 maintained potent antibacterial and biofilm ablation effects against this strain, outperforming free Ce6. This sustained efficacy arises from ABP's reversible adsorption, which still enables proximity-driven Ce6 delivery to the target bacteria. In vivo, the ABP-Ce6 significantly enhanced mice wound healing for infections caused by CRAB Ab1513 and Ab1513-BIM12. In conclusion, ABP-Ce6 exhibits significant efficacy as a therapeutic agent against CRAB infections even after the bacteria develop resistance to phage therapy. This novel strategy may serve as a hopeful complementary strategy to phage therapy, thereby reducing delays in screening for new therapeutic phages.

耐碳青霉烯鲍曼不动杆菌（CRAB）经常引起生物膜相关感染，对抗生素治疗表现出极大的耐药性。噬菌体疗法有望成为一种替代疗法，但细菌可能会因长期使用而产生耐药性。噬菌体-光敏剂联合治疗是一种新的抗菌策略。本研究旨在评价氯e6功能化噬菌体清除生物膜和治疗螃蟹感染的效果，并评估其在噬菌体耐药性出现后的持续后效。成功合成了鲍曼芽胞杆菌噬菌体(ABP)-氯e6偶联物（ABP- ce6）并对其进行了表征。它保留了噬菌体的吸收能力和裂解活性，同时提高了活性氧（ROS）的产生。此外，ABP-Ce6具有与ABP相当且超过Ce6的显著抗菌活性，在抑制螃蟹Ab1513生物膜形成和破坏已有生物膜方面均表现出优异的性能。值得注意的是，尽管ABP由于无法实现不可逆吸附而对抗噬菌体螃蟹Ab1513-BIM12没有效果，但ABP-Ce6对该菌株保持了有效的抗菌和生物膜消融作用，优于游离Ce6。这种持续的功效源于ABP的可逆吸附，它仍然可以使接近驱动的Ce6递送到目标细菌。在体内，ABP-Ce6显著促进了CRAB Ab1513和Ab1513- bim12引起的小鼠伤口愈合。综上所述，即使在细菌对噬菌体治疗产生耐药性后，ABP-Ce6作为治疗螃蟹感染的药物仍具有显著的疗效。这种新策略可能作为噬菌体治疗的一种有希望的补充策略，从而减少筛选新的治疗噬菌体的延迟。

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引用次数: 0

Triphenylamine-based fluorescent probes for lipid droplet detection and non-alcoholic fatty liver disease identification 基于三苯胺的荧光探针用于脂滴检测和非酒精性脂肪性肝病的鉴定。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-04 DOI: 10.1016/j.jphotobiol.2025.113301

Jing-Yi Li , Ya-Tong Liu , Zhi-Bo Jiang, Si-Yi Yao, Fan-Qi Shen, Yu-Yang Wang, Yue-Li Zou, Li-Xia Zhao

Lipid droplets (LDs) are important biomarkers for metabolic diseases such as non-alcoholic fatty liver disease (NAFLD), and they have significant research value in revealing the pathological mechanisms of these diseases. In this study, we designed three fluorescent probes based on triphenylamine, namely DM-1, DM-2, and DM-3, for the targeted detection of lipid droplets and the identification of NAFLD. DM1–3 exhibits a unique response to small polar environments and has high sensitivity, high selectivity, large Stokes shift, high fluorescence quantum yield, a wide pH range (2–8), and rapid recognition. Particularly, the fluorescence intensity of probe DM-1 at 576 nm (expressed in logarithmic form) has a good linear relationship with PBS/Diox (80–96 %) (R² = 0.9888). Therefore, probe DM-1 was selected as the object for further study. The results of cell experiments indicated that the probe DM-1 could target lipid droplets within cells and exhibited low cytotoxicity. In vivo experiments successfully achieved the imaging of LDs in the liver of NAFLD mouse models at different stages. Together, these results demonstrated the potential of probe DM-1 as a polar-sensitive fluorescent tool for the early diagnosis and monitoring of NAFLD.

脂滴（ld）是非酒精性脂肪性肝病（NAFLD）等代谢性疾病的重要生物标志物，在揭示这些疾病的病理机制方面具有重要的研究价值。本研究设计了3种基于三苯胺的荧光探针DM-1、DM-2和DM-3，用于脂滴的靶向检测和NAFLD的鉴定。DM1-3对小极性环境具有高灵敏度、高选择性、Stokes位移大、荧光量子产率高、pH范围宽（2-8）、识别速度快等特点。特别是探针DM-1在576 nm处的荧光强度（以对数形式表示）与PBS/Diox呈良好的线性关系（80- 96%）（R2 = 0.9888）。因此，选择探针DM-1作为进一步研究的对象。细胞实验结果表明，探针DM-1可以靶向细胞内脂滴，具有较低的细胞毒性。体内实验成功实现了NAFLD小鼠模型不同阶段肝脏ld的成像。总之，这些结果证明了探针DM-1作为NAFLD早期诊断和监测的极敏荧光工具的潜力。

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引用次数: 0

Light-induced reproductive responses in white-rumped munia (Lonchura striata) 白翅文虫的光诱导生殖反应

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-03 DOI: 10.1016/j.jphotobiol.2025.113300

Ruth Lalpekhlui, Lalsiamkima Hnamler, Amit Kumar Trivedi

Photoperiod is a primary cue for most birds to initiate reproductive activities. How light influences the reproduction-linked processes in white-rumped munia is largely unknown. A series of experiments were performed on male birds. In experiment one, birds were exposed to either short (SD; 8 L: 16D) or long day length (LD; 15 L:9D) for one day and sampled at 19 h after light onset. In experiment two, birds were exposed to either SD (8 L: 16D), or equinox photoperiod (12 L:12D), or to LD (15 L:9D). In experiment 3, birds were exposed to an equinox photoperiod of 10 lx, 50 lx, or 100 lx. In experiment four, birds were exposed to an equinox photoperiod (0.5 W/m² of light irradiance) of red (RL; 650 nm) or blue light (BL; 450 nm). Experiment 2 to 4 ran for 30 days. For experiment five, birds were procured at four different times of the year, i.e., March, June, September, and December. Body mass and testicular volume size were observed in all experiments. In experiment 2 to 5 sampling was done during the middle of the light phase. Hypothalamic tissue was used for transcription studies of reproductive and epigenetic markers. In experiment 1, higher expressions of Tshβ, Dio2, Eya3, Tet2, and Hat1 while lower expression of Dnmt1 was observed in LD group. In experiment 2, higher expression of Tshβ, Dio2, GnRh, Eya3, and Tet2 while lower expression of of Dio3, GnIh, Hdac2, Dnmt1, and Dnmt3b was observed in both 12 L:12D and 15 L:9D LD groups. In experiment 3, higher expression of Tshβ, Dio2, GnRh, Tet2, and Hat1 while lower expression of Dio3, GnIh, Hdac2, and Dnmt3b was revealed in 50 and 100 lx groups. Experiment 4 resulted in higher expression of Tshβ, Dio2, GnRh, and Tet2 while lower expression of Dio3, GnIh, Hdac2, Dnmt1 and Dnmt3b in blue light group. Seasonal fluctuations were observed in all hypothalamic transcripts studied. Higher expression of Tshβ, Dio2, GnRh, Eya3, Tet2, and Hat1 was revealed in September while Dio3, GnIh, Hdac2, Dnmt1 and Dnmt3b were higher at other time of the year. The results from experiment 1 to 4 suggest that all three components of light i.e., duration, illuminance and spectral composition of light affects reproductive responses. While in nature season controls reproductive responses. Change in epigenetic transcripts suggest that and these responses are mediated by epigenetic mechanisms.

光周期是大多数鸟类开始繁殖活动的主要线索。光是如何影响白腹文虫的繁殖过程的，这在很大程度上是未知的。对雄性鸟类进行了一系列实验。在实验一中，将雏鸟暴露在短（SD; 8 L: 16D）或长（LD; 15 L:9D）的环境中1天，并在光照开始后19 h取样。在实验二中，鸟类暴露于SD （8 L: 16D）或春分光周期（12 L:12D）或LD （15 L:9D）。在实验3中，鸟类被暴露在10 lx、50 lx或100 lx的春分光周期中。在实验四中，将鸟类暴露在平分点光周期（0.5 W/m2光辐照度）的红光（RL; 650 nm）或蓝光（BL; 450 nm）下。试验2 ~试验4运行30 d。实验五在一年中的4个不同时间，即3月、6月、9月和12月进行捕鸟。所有实验均观察体质量和睾丸体积大小。在实验2到5中，采样是在光期的中间进行的。下丘脑组织用于生殖和表观遗传标记的转录研究。实验1中，LD组Tshβ、Dio2、Eya3、Tet2、Hat1表达升高，Dnmt1表达降低。实验2中，12 L:12D和15 L:9D LD组Tshβ、Dio2、GnRh、Eya3、Tet2的表达均较高，而Dio3、GnIh、Hdac2、Dnmt1、Dnmt3b的表达均较低。实验3中，50和100 lx组Tshβ、Dio2、GnRh、Tet2和Hat1的表达较高，而Dio3、GnIh、Hdac2和Dnmt3b的表达较低。实验4显示蓝光组Tshβ、Dio2、GnRh、Tet2表达升高，而Dio3、GnIh、Hdac2、Dnmt1、Dnmt3b表达降低。在所有研究的下丘脑转录本中均观察到季节性波动。Tshβ、Dio2、GnRh、Eya3、Tet2和Hat1的表达在9月份较高，而Dio3、GnIh、Hdac2、Dnmt1和Dnmt3b的表达在其他月份较高。实验1 - 4的结果表明，光的三个组成部分，即光的持续时间、照度和光谱组成都影响生殖反应。而在自然界中，季节控制着繁殖反应。表观遗传转录物的变化表明，这些反应是由表观遗传机制介导的。

{"title":"Light-induced reproductive responses in white-rumped munia (Lonchura striata)","authors":"Ruth Lalpekhlui, Lalsiamkima Hnamler, Amit Kumar Trivedi","doi":"10.1016/j.jphotobiol.2025.113300","DOIUrl":"10.1016/j.jphotobiol.2025.113300","url":null,"abstract":"<div><div>Photoperiod is a primary cue for most birds to initiate reproductive activities. How light influences the reproduction-linked processes in white-rumped munia is largely unknown. A series of experiments were performed on male birds. In experiment one, birds were exposed to either short (SD; 8 L: 16D) or long day length (LD; 15 L:9D) for one day and sampled at 19 h after light onset. In experiment two, birds were exposed to either SD (8 L: 16D), or equinox photoperiod (12 L:12D), or to LD (15 L:9D). In experiment 3, birds were exposed to an equinox photoperiod of 10 lx, 50 lx, or 100 lx. In experiment four, birds were exposed to an equinox photoperiod (0.5 W/m<sup>2</sup> of light irradiance) of red (RL; 650 nm) or blue light (BL; 450 nm). Experiment 2 to 4 ran for 30 days. For experiment five, birds were procured at four different times of the year, i.e., March, June, September, and December. Body mass and testicular volume size were observed in all experiments. In experiment 2 to 5 sampling was done during the middle of the light phase. Hypothalamic tissue was used for transcription studies of reproductive and epigenetic markers. In experiment 1, higher expressions of <em>Tshβ</em>, <em>Dio2</em>, <em>Eya3</em>, <em>Tet2</em>, and <em>Hat1</em> while lower expression of <em>Dnmt1</em> was observed in LD group. In experiment 2, higher expression of <em>Tshβ</em>, <em>Dio2</em>, <em>GnRh</em>, <em>Eya3</em>, and <em>Tet2</em> while lower expression of of <em>Dio3</em>, <em>GnIh</em>, <em>Hdac2</em>, <em>Dnmt1,</em> and <em>Dnmt3b</em> was observed in both 12 L:12D and 15 L:9D LD groups. In experiment 3, higher expression of <em>Tshβ</em>, <em>Dio2</em>, <em>GnRh</em>, <em>Tet2</em>, and <em>Hat1</em> while lower expression of <em>Dio3</em>, <em>GnIh</em>, <em>Hdac2</em>, and <em>Dnmt3b</em> was revealed in 50 and 100 lx groups. Experiment 4 resulted in higher expression of <em>Tshβ</em>, <em>Dio2</em>, <em>GnRh</em>, and <em>Tet2</em> while lower expression of <em>Dio3</em>, <em>GnIh</em>, <em>Hdac2</em>, <em>Dnmt1</em> and <em>Dnmt3b</em> in blue light group. Seasonal fluctuations were observed in all hypothalamic transcripts studied. Higher expression of <em>Tshβ</em>, <em>Dio2</em>, <em>GnRh</em>, <em>Eya3</em>, <em>Tet2</em>, and <em>Hat1</em> was revealed in September while <em>Dio3</em>, <em>GnIh</em>, <em>Hdac2</em>, <em>Dnmt1</em> and <em>Dnmt3b</em> were higher at other time of the year. The results from experiment 1 to 4 suggest that all three components of light i.e., duration, illuminance and spectral composition of light affects reproductive responses. While in nature season controls reproductive responses. Change in epigenetic transcripts suggest that and these responses are mediated by epigenetic mechanisms.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"273 ","pages":"Article 113300"},"PeriodicalIF":3.7,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145468091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sonodynamic and photodynamic therapy, activated indocyanine green, and indocyanine green-loaded chitosan nanoparticle on resin tag length and shear bond strength to caries-affected dentin using two-step etch and rinse resin adhesive 超声动力和光动力疗法，激活吲哚菁绿，以及负载吲哚菁绿的壳聚糖纳米粒子对树脂标签长度和剪切结合强度的影响，采用两步蚀刻和冲洗树脂粘合剂。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-02 DOI: 10.1016/j.jphotobiol.2025.113291

Fahad Alkhudhairy

AIM

Evaluating the effect of different disinfection protocols, i.e., Chlorhexidine (CHX), Sonodynamic therapy (SDT)-Indocyanine green (ICG), photodynamic therapy (PDT)-ICG, and ICG-loaded chitosan nanoparticles (CHNPs) on the resin tag length (RTL) and the shear bond strength (SBS) bonded to caries-affected dentin (CAD) using two step etch and rinse resin adhesive.

Materials and methods

The present study utilized fifty-six extracted human molars with carious lesions extending up to halfway between the pulp chamber and the enamel-dentin junction, corresponding to ICDAS criteria 5. All the samples were allocated into four groups based on the disinfection regimen (n = 14). Group 1: CHX, Group 2: ICG-PDT, Group 3: ICG-SDT, and Group 4: ICG-loaded CHNPs. A traditional two-step etch and rinse adhesive was used, followed by composite buildup. All specimens underwent thermocycling to replicate the aging effect. Scanning electron microscopy was used for characterization of chitosan nanoparticles and ICG-loaded CHNPs, followed by measurement of RTL (n = 4). Failure mode assessment and SBS analysis were performed using a stereomicroscope and universal testing machine(n = 10). ANOVA2 and post hoc Tukey test were conducted to statistically compare the RTL and SBS outcomes among various investigated groups, p ˂0.05.

Results

Group 3 (ICG-SDT) samples presented the maximum length of resin tags (121.82 ± 5.23 μm) and the highest bond strength (9.43 ± 0.23 MPa). Whereas the minimum resin tag length was detected in Group 2 (ICG-PDT) (59.21 ± 2.11 μm), along with the lowest bond strength (6.41 ± 0.92 MPa). Comparative analysis between Group 1 (CHX) and Group 2 indicated that no significant difference in their resin tag length and SBS (p˃0.05). Similarly, intergroup analysis between Group 3 and Group 4 also demonstrated comparable bond strength and resin tags (p˃0.05).

Conclusion

Sonodynamic therapy with indocyanine green and indocyanine green-infused chitosan nanoparticles is an effective method for disinfecting caries-affected dentin, as it has shown appropriate resin tag length and bond strength.

目的：评价氯己定（CHX）、声动力疗法(SDT)-吲哚菁绿（ICG）、光动力疗法(PDT)-ICG和负载ICG的壳聚糖纳米颗粒（CHNPs）不同消毒方案对树脂标签长度（RTL）和两步蚀刻和冲洗树脂粘合剂与龋病牙本质（CAD）的剪切结合强度（SBS）的影响。材料和方法：本研究使用56颗被拔出的人磨牙，龋齿病变延伸到牙髓腔和牙釉质交界处之间的一半，符合ICDAS标准5。所有样本按消毒方案分为4组（n = 14）。第1组：CHX，第2组：ICG-PDT，第3组：ICG-SDT，第4组：icg加载CHNPs。使用传统的两步蚀刻和漂洗粘合剂，然后是复合材料堆积。所有标本都进行了热循环以复制老化效应。利用扫描电镜对壳聚糖纳米粒子和负载icg的CHNPs进行表征，并测量RTL （n = 4）。采用体视显微镜和万能试验机进行失效模式评估和SBS分析（n = 10）。采用ANOVA2和事后Tukey检验对各调查组RTL和SBS结果进行统计学比较，p < 0.05。结果：第3组（ICG-SDT）树脂标签长度最大（121.82±5.23 μm），结合强度最高（9.43±0.23 MPa）。第2组（ICG-PDT）树脂标签长度最小（59.21±2.11 μm），结合强度最低（6.41±0.92 MPa）。1组（CHX）与2组的树脂标签长度和SBS比较，差异无统计学意义（p > 0.05）。同样，组3和组4之间的组间分析也显示出相似的粘合强度和树脂标签（p < 0.05）。结论：吲哚菁绿和吲哚菁绿注入壳聚糖纳米粒子声动力治疗龋病牙本质是一种有效的消毒方法，具有合适的树脂标签长度和结合强度。

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引用次数: 0

Unimodal and bimodal classification methods for breast carcinomas based on laser-induced autofluorescence spectroscopy 基于激光诱导自体荧光光谱的乳腺癌单峰和双峰分类方法

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-01 DOI: 10.1016/j.jphotobiol.2025.113299

Dedong Guo , Zewei Ouyang , Baichuan Long , Guanwei Zeng , Zhuocheng Chen , Maoliang Chen , Junlang Chen , Xing Zhang , Jianfeng Yan , Pusheng Zhang , Zhifeng Chen

Despite its efficiency, real-time capability, and low cost, laser-induced fluorescence spectroscopy has limited classification accuracy in breast carcinoma diagnosis, restricting its clinical application. To address this, we evaluated three steady-state autofluorescence analysis approaches: spectral ratio, piecewise linear fitting, and univariate cubic polynomial fitting. To make the analysis more systematic and objective, a sliding-window mechanism and statistical difference analysis were introduced. Based on these results, a novel composite-feature strategy was proposed through arithmetic combination of slope values across multiple spectral segments, enabling distributed spectral information to be effectively integrated and enhancing classification stability. Under the current sample size, piecewise linear fitting demonstrated superior performance by extracting multiple slope features, one of which achieved 100 % classification. However, ambiguous boundaries limited unimodal generalizability for larger datasets. We then combined the optimal unimodal method—piecewise linear fitting—with time-resolved fluorescence lifetime data for bimodal analysis. This fusion markedly enhanced class separability over unimodal approaches. Furthermore, decision boundaries from support vector machines (SVM) were sharper than those from linear discriminant analysis (LDA). These findings highlight the diagnostic value of spectral slope features and emphasize the enhanced classification performance achieved by combining steady-state with time-resolved data. The proposed method is label-free, low-cost, time-efficient, and shows strong potential for intelligent diagnostics by reducing reliance on subjective interpretation. The observed differences likely arise from structural protein changes (collagen, elastin), altered protein-bound NAD(P)H ratios, elevated FAD, and porphyrin accumulation, reflecting tumor-related metabolic and microstructural changes.

激光诱导荧光光谱在乳腺癌诊断中的分类准确性有限，限制了其临床应用，但其效率高、实时性好、成本低。为了解决这个问题，我们评估了三种稳态自荧光分析方法：光谱比、分段线性拟合和单变量三次多项式拟合。为了使分析更加系统和客观，引入了滑动窗口机制和统计差异分析。在此基础上，提出了一种基于多光谱段坡度值的复合特征策略，实现了对分布式光谱信息的有效整合，提高了分类的稳定性。在当前样本量下，分段线性拟合通过提取多个斜率特征表现出较好的性能，其中一个特征的分类率达到100%。然而，模糊边界限制了大型数据集的单峰泛化性。然后，我们将最佳单峰方法-分段线性拟合-与时间分辨荧光寿命数据相结合进行双峰分析。与单模方法相比，这种融合显著增强了类的可分离性。此外，支持向量机（SVM）的决策边界比线性判别分析（LDA）的决策边界更清晰。这些发现突出了光谱斜率特征的诊断价值，并强调了将稳态数据与时间分辨数据相结合所获得的增强分类性能。该方法无标签，低成本，省时，并且通过减少对主观解释的依赖，显示出强大的智能诊断潜力。观察到的差异可能是由于结构蛋白的变化（胶原蛋白，弹性蛋白），蛋白质结合的NAD(P)H比率的改变，FAD升高和卟啉积累，反映了肿瘤相关的代谢和微观结构变化。

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引用次数: 0

Improvement of the antimicrobial efficacy of high-intensity blue light against Pseudomonas aeruginosa by discontinuous irradiation 间断照射提高高强度蓝光对铜绿假单胞菌抑菌效果的研究

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-30 DOI: 10.1016/j.jphotobiol.2025.113288

Tara Fardoust , Isabell Blassnig , Paul C. Fuchs , Jennifer L. Schiefer , Christian Opländer

Wound infections caused by Pseudomonas aeruginosa (PA) are a common cause of death due to subsequent sepsis in immunosuppressed burn patients. Blue light at 455 nm exhibits antibacterial properties against PA and may offer a therapeutic option for treating such infections. However, low intensities require longer treatment durations, while high intensities cause temperature-related challenges. We therefore investigated the antibacterial efficacy of discontinuous blue light treatment (DBLT) at high intensity.

DBLT cycles (up to 8) comprised 3 min of irradiation (300 mW/cm²) followed by a 2, 4, or 8 min pause to deliver the same doses (up to 432 J/cm²) as continuous blue light treatment (CLBT). In suspension assays, DBLT with 4-min pauses was most effective, achieving complete bacterial eradication after 5 cycles, whereas CLBT at the same dose (270 J/cm²) resulted in only a 1-log-level reduction. In a wound contamination model, DBLT (8 cycles, 4-min pauses) reduced bacterial load by 5 log levels, whereas CLBT only reduced it by 1–2 log levels.

Flow cytometry and the redox-sensitive probe DCFH-DA assessed blue light-induced oxidative stress in PA. DBLT caused more oxidative stress (2-fold) than CLBT. This suggests that pauses in irradiation enhance the production of reactive species, which may accumulate and affect bacterial vitality.

In conclusion, incorporating defined pause intervals into blue light therapy enhances antibacterial effectiveness, reduces heat development, and may improve clinical outcomes for bacterial infections in burn patients.

由铜绿假单胞菌（PA）引起的伤口感染是免疫抑制烧伤患者因脓毒症而死亡的常见原因。455nm的蓝光对PA具有抗菌特性，可能为治疗此类感染提供治疗选择。然而，低强度需要更长的处理时间，而高强度会导致与温度相关的挑战。因此，我们研究了高强度间断蓝光治疗（DBLT）的抗菌效果。DBLT循环（最多8次）包括3分钟的照射（300 mW/cm2），然后暂停2、4或8分钟，以提供与连续蓝光治疗（CLBT）相同的剂量（最多432 J/cm2）。在悬浮液试验中，暂停4分钟的DBLT最有效，在5个循环后完全根除细菌，而相同剂量（270 J/cm2）的CLBT仅导致1个对数水平的减少。在伤口污染模型中，DBLT（8个循环，暂停4分钟）减少了5个日志级别的细菌负荷，而CLBT只减少了1-2个日志级别。流式细胞术和氧化还原敏感探针DCFH-DA评估蓝光诱导的PA氧化应激。DBLT比CLBT引起更多的氧化应激（2倍）。这表明，暂停辐照会增加活性物质的产生，这些活性物质可能会积累并影响细菌的活力。综上所述，在蓝光治疗中加入规定的暂停时间可以提高抗菌效果，减少热的产生，并可能改善烧伤患者细菌感染的临床结果。

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引用次数: 0

Synthesis and characterization of multifunctional mesoporous silica nanoparticles with dual targeting and dual-mode imaging for cancer therapy 用于癌症治疗的多功能介孔二氧化硅纳米颗粒的合成和表征

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-28 DOI: 10.1016/j.jphotobiol.2025.113290

Shiow-Yi Chen, Kian-Keat Kwek, Zui Harng Lee, Hsiu-Mei Lin

This study investigated the innovative potential of multifunctional mesoporous silica nanoparticles (MSNs) as a novel drug carrier for cancer therapy. Dual-targeting was achieved by conjugating folic acid (FA) and glucose, enabling selective binding to folate receptors and glucose transporters on cancer cells. Europium (Eu³⁺) and gadolinium (Gd³⁺), were doped onto the MSN to provide fluorescence and MRI dual-mode imaging, while camptothecin (CPT) was attached via disulfide bonds for glutathione (GSH)-responsive release. Characterization confirmed the mesoporous structure and successful functionalization. In vitro assays showed that MSN-EuGd-CPT-Glu-FA exhibited good biocompatibility, maintaining over 80 % viability in normal L929 cells at 200 μg/mL, while significantly enhancing cytotoxicity toward HeLa cells, where viability decreased to ∼40 % and the IC₅₀ value dropped from 118.66 μg/mL for free CPT to 8.31 μg/mL. In vivo studies further validated the dual-mode imaging capability in mice using IVIS and MRI. These results demonstrate that the designed MSN system integrates targeted delivery, stimuli-responsive drug release, and diagnostic imaging, offering a promising multifunctional nanoplatform with the potential to enhance therapeutic efficacy while reducing side effects in cancer treatment.

本研究探讨了多功能介孔二氧化硅纳米颗粒（MSNs）作为癌症治疗的新型药物载体的创新潜力。双靶向是通过叶酸（FA）和葡萄糖偶联实现的，可以选择性地结合癌细胞上的叶酸受体和葡萄糖转运体。将铕（Eu3+）和钆（Gd3+）掺杂到MSN上，提供荧光和MRI双模成像，而喜树碱（CPT）通过二硫键连接，以释放谷胱甘肽（GSH）。表征证实了介孔结构和成功的功能化。体外实验表明，mnn - eug -CPT- glu - fa具有良好的生物相容性，在200 μg/mL时在正常L929细胞中保持80%以上的活力，同时显著增强了对HeLa细胞的细胞毒性，其中活力降至~ 40%，IC₅0值从游离CPT的118.66 μg/mL降至8.31 μg/mL。体内研究进一步验证了小鼠IVIS和MRI的双模成像能力。这些结果表明，所设计的MSN系统集成了靶向递送、刺激反应性药物释放和诊断成像，提供了一个有前途的多功能纳米平台，在癌症治疗中具有提高疗效和减少副作用的潜力。

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引用次数: 0

Photoactivity of Pluronic® F-127 nanoencapsulated hypericin against Leishmania: A promising approach for cutaneous leishmaniasis Pluronic®F-127纳米包封金丝桃素抗利什曼病的光活性：一种治疗皮肤利什曼病的有前景的方法

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-28 DOI: 10.1016/j.jphotobiol.2025.113292

Larissa Ferreira de Oliveira , Alanis Boer dos Reis , Ana Paula Rizzo Bueno , Tiffany Suzawa , Maria Julia Schiavon Gonçalves , Anibal Salinas Junior , Ana Carolina Vieira de Oliveira , Paulo Cesar Souza Pereira , Wilker Caetano , Flavio Augusto Vicente Seixas , Áquila Carolina Fernandes Herculano Ramos-Milaré , Daniele Stefanie Sara Lopes Lera-Nonose , Maria Valdrinez Campana Lonardoni

Hypericin (HYP) is a naturally occurring naphthodianthrone that has been extensively studied, particularly in combination with photodynamic therapy (PDT), for its therapeutic properties. Encapsulation of HYP with Pluronic® F-127 nanoparticles enhances its bioavailability. Leishmaniasis is a neglected disease and a global health problem, with varying clinical forms caused by Leishmania parasites. Current treatments are limited by high toxicity and relapse, highlighting the need for alternative therapeutics. We investigated the activity of free HYP and that encapsulated with Pluronic® F-127 (HYPF127) in association with PDT against Leishmania (Leishmania) amazonensis. These compounds demonstrated high anti-Leishmania activity when associated with PDT, with IC₅₀ values for promastigote forms of 18 ± 3 μM for HYP and 16 ± 5 μM for HYPF127. Moderate activity against amastigotes was observed at lower concentrations, and prominent macrophage cytotoxicity was detected in the compounds following irradiation, with CC₅₀ of 0.6 ± 0.2 μM for HYPF127 and 0.2 ± 0.1 μM for HYP in murine macrophages. Phosphatidylserine externalization, mitochondrial membrane depolarization, and DNA fragmentation were observed in promastigotes treated with PDT, suggesting the induction of apoptosis. These results are slightly superior to those obtained for HYPF127. In silico evaluation demonstrated the affinity of HYP for trypanothione reductase (TR), suggesting a possible target and mechanism of action through redox homeostasis inhibition in Leishmania. In conclusion, nanoencapsulated photoactivated HYP exhibited promising antileishmanial activity against L. amazonensis, with F-127 enhancing its potential. Therefore, TR inhibition may be involved in the mechanism of action.

金丝桃素（Hypericin， HYP）是一种天然存在的萘醌，因其治疗特性而被广泛研究，特别是与光动力疗法（PDT）结合使用。Pluronic®F-127纳米颗粒包封HYP可提高其生物利用度。利什曼病是一种被忽视的疾病，也是一个全球卫生问题，由利什曼原虫引起的临床形式各不相同。目前的治疗受到高毒性和复发的限制，突出了替代治疗的必要性。我们研究了游离HYP和Pluronic®F-127包封的HYP （HYPF127）与PDT对亚马逊利什曼原虫（Leishmania）的作用。这些化合物与PDT结合时显示出较高的抗利什曼原虫活性，对promastigote形式的HYP和HYPF127的IC50值分别为18±3 μM和16±5 μM。在较低浓度下，化合物对无尾线虫有中等活性，辐照后对小鼠巨噬细胞有明显的细胞毒性，对HYPF127的CC50为0.6±0.2 μM，对HYP的CC50为0.2±0.1 μM。在PDT处理的原乳螺体中观察到磷脂酰丝氨酸外化，线粒体膜去极化和DNA断裂，提示诱导细胞凋亡。这些结果略优于HYPF127的结果。计算机评价表明HYP对锥虫硫酮还原酶（TR）具有亲和力，提示其可能的靶点和作用机制是通过抑制利什曼原虫的氧化还原稳态。综上所述，纳米包封的光活化HYP对亚马逊河蛭具有良好的抗利什曼原虫活性，其中F-127增强了其抗利什曼原虫活性。因此，TR抑制可能参与了其作用机制。

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引用次数: 0