Journal of photochemistry and photobiology. B, Biology最新文献_第6页

Corrigendum to “An oxygen-independent therapeutic nanosystem for fighting against hypoxic and antioxidant microenvironment of tumor” [Journal of Photochemistry and Photobiology B: Biology 268 (2025) 113184] “一种不依赖氧的治疗性纳米系统，用于对抗肿瘤的缺氧和抗氧化微环境”[j].光化学与光生物学报，2008(5):113184。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2026-01-01 Epub Date: 2025-12-06 DOI: 10.1016/j.jphotobiol.2025.113332

Zixuan Wang , Shuwei Nie , Manru Wang , Huina Niu , Liqi Wei , Zhiqi Yang , Xin Liu , Yining Chen , Yunan Yang , Chunjiang Li , Qin Zhang , Lina Feng , Hongxia Ma , Rui Chen , Yan Cheng

引用次数: 0

Priming cancer cells via photobiomodulation at NIR wavelength to enhance photodynamic action: Insights into mechanistic alterations and cellular migration 通过近红外波长的光生物调节来激活癌细胞以增强光动力作用：机制改变和细胞迁移的见解

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2026-01-01 Epub Date: 2025-12-11 DOI: 10.1016/j.jphotobiol.2025.113336

Büşra Sirek , Nermin Topaloğlu

Photodynamic therapy (PDT) is a successful therapy for cancer treatment, especially for the superficial and easily reachable types, such as prostate cancer. During photodynamic activation, the light-sensitive chemical, which generally does not have a toxic effect on cells, can induce oxidative stress by producing reactive oxygen species (ROS). Nevertheless, the outcome of the therapy may be diminished by certain drawbacks. For this, PDT can be coupled with other therapies. Different light therapies can also serve as effective anticancer strategies. The combination of photobiomodulation (PBM) with PDT has become increasingly popular in cancer management. In the present study, PBM, which lacks anticancer activity, was combined with Chlorin e6 (Ce6)-mediated PDT. Underlying causes of additional deaths were elucidated by various mechanistic analyses, including ROS, nitric oxide (NO), mitochondrial membrane potential (ΔΨm), etc. PBM priming at an energy density of 5 J/cm² resulted in up to 64 % additional cell death, as demonstrated by colorimetric and dual-staining analyses, and nearly a 100 % decrease in cell migration compared to PDT alone. Besides, PBM priming induced an additional 0.5-fold reduction in NO levels, a 19.4 % increase in ROS levels, and a 1.2 % reduction in ΔΨm compared to only PDT applications. Thus, combining PDT with PBM priming can provide a more effective therapeutic approach and significantly diminish the invasiveness of cancer cells.

光动力疗法（PDT）是一种成功的癌症治疗方法，特别是对于表面和容易到达的类型，如前列腺癌。在光动力激活过程中，通常对细胞没有毒性作用的光敏化学物质可以通过产生活性氧（ROS）诱导氧化应激。然而，这种疗法的效果可能会因某些缺陷而降低。为此，PDT可以与其他疗法结合使用。不同的光疗法也可以作为有效的抗癌策略。光生物调节（PBM）与PDT的结合在癌症治疗中越来越受欢迎。在本研究中，缺乏抗癌活性的PBM与氯e6 （Ce6）介导的PDT联合使用。通过各种机制分析，包括活性氧、一氧化氮（NO）、线粒体膜电位（ΔΨm）等，阐明了额外死亡的潜在原因。比色法和双染色分析表明，以5 J/cm2的能量密度启动PBM可导致高达64%的额外细胞死亡，并且与单独使用PDT相比，细胞迁移减少了近100%。此外，与仅应用PDT相比，PBM启动诱导NO水平降低0.5倍，ROS水平增加19.4%，ΔΨm降低1.2%。因此，PDT联合PBM启动可以提供更有效的治疗方法，并显著降低癌细胞的侵袭性。

{"title":"Priming cancer cells via photobiomodulation at NIR wavelength to enhance photodynamic action: Insights into mechanistic alterations and cellular migration","authors":"Büşra Sirek , Nermin Topaloğlu","doi":"10.1016/j.jphotobiol.2025.113336","DOIUrl":"10.1016/j.jphotobiol.2025.113336","url":null,"abstract":"<div><div>Photodynamic therapy (PDT) is a successful therapy for cancer treatment, especially for the superficial and easily reachable types, such as prostate cancer. During photodynamic activation, the light-sensitive chemical, which generally does not have a toxic effect on cells, can induce oxidative stress by producing reactive oxygen species (ROS). Nevertheless, the outcome of the therapy may be diminished by certain drawbacks. For this, PDT can be coupled with other therapies. Different light therapies can also serve as effective anticancer strategies. The combination of photobiomodulation (PBM) with PDT has become increasingly popular in cancer management. In the present study, PBM, which lacks anticancer activity, was combined with Chlorin e6 (Ce6)-mediated PDT. Underlying causes of additional deaths were elucidated by various mechanistic analyses, including ROS, nitric oxide (NO), mitochondrial membrane potential (ΔΨm), etc. PBM priming at an energy density of 5 J/cm<sup>2</sup> resulted in up to 64 % additional cell death, as demonstrated by colorimetric and dual-staining analyses, and nearly a 100 % decrease in cell migration compared to PDT alone. Besides, PBM priming induced an additional 0.5-fold reduction in NO levels, a 19.4 % increase in ROS levels, and a 1.2 % reduction in ΔΨm compared to only PDT applications. Thus, combining PDT with PBM priming can provide a more effective therapeutic approach and significantly diminish the invasiveness of cancer cells.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113336"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intermittent blue light-dark cycles: A new strategy for enhancing triterpenoid production in medicinal Sanghuangporus vaninii 间歇性蓝-暗循环：提高药用桑黄茯苓三萜产量的新策略。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2026-01-01 Epub Date: 2025-12-11 DOI: 10.1016/j.jphotobiol.2025.113338

Yanjun Ma , Rong Li , Jiahui Li , Zilu Song , Fawen She , Xiaoyan Ma , Xuetai Zhu

Triterpenoids from medicinal fungi have significant biological activities and industrial potential, but their low yield limits large-scale production. Sanghuangporus vaninii, a widely cultivated medicinal fungus, is a promising source of triterpenoids, yet the regulatory role of light-dark cycles in its triterpenoid biosynthesis remains unclear. In this study, we demonstrated that intermittent intense blue light-dark cycles (IB/D; ∼110 μmol/m²·s, 10 h/14 h) dramatically enhance triterpenoid production in S. vaninii MF5. IB/D treatment increased triterpenoid yield by 154 % compared to dark controls, also outperforming constant light or white light-dark regimens. Mechanistically, IB/D was associated with: (i) hyperbranching morphology (42 % reduced internode length); (ii) membrane remodeling via upregulated fatty acid desaturases and transporters, enhancing permeability 2.88-fold; and (iii) transcriptional activation of the mevalonate pathway, which was accompanied by a dramatic induction of endogenous 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) expression 4497-fold. Subsequent cloning and characterization of the SvHMGS gene-through sequence homology with known HMGS genes from Sanghuangporus species, identification of conserved HMGS domains, and prediction of its association with the mevalonic acid (MVA) pathway-suggested its potential conserved function in triterpenoid biosynthesis. This photoperiodic strategy represents a paradigm shift from constant light suppression to rhythmic induction, offering a scalable, low-cost approach to boost triterpenoid production in this medicinal fungus.

药用真菌中的三萜具有显著的生物活性和工业潜力，但其产量低，限制了其大规模生产。桑黄孢是一种广泛种植的药用真菌，是一种很有前途的三萜来源，但光暗循环在其三萜生物合成中的调节作用尚不清楚。在这项研究中，我们证明了间歇性强蓝光-暗循环（IB/D; ~ 110 μmol/m2·s, 10 h/14 h）显著提高了s . vaninii MF5的三萜产量。与黑暗对照相比，IB/D处理增加了154%的三萜产量，也优于恒定光照或白光-黑暗方案。在机制上，IB/D与：(i)超分枝形态（节间长度减少42%）；（ii）通过脂肪酸去饱和酶和转运蛋白上调的膜重塑，使通透性提高2.88倍；（iii）甲羟戊酸途径的转录激活，这伴随着内源性3-羟基-3-甲基戊二酰辅酶a合成酶（HMGS）表达的显著诱导4497倍。随后，通过与桑黄孢子属已知HMGS基因的序列同源性、鉴定保守的HMGS结构域以及预测其与甲羟戊酸（MVA）途径的关联，对SvHMGS基因进行了克隆和鉴定，表明其在三萜生物合成中可能具有保守功能。这种光周期策略代表了从恒定的光抑制到有节奏的诱导的范式转变，提供了一种可扩展的、低成本的方法来促进这种药用真菌的三萜生产。

{"title":"Intermittent blue light-dark cycles: A new strategy for enhancing triterpenoid production in medicinal Sanghuangporus vaninii","authors":"Yanjun Ma , Rong Li , Jiahui Li , Zilu Song , Fawen She , Xiaoyan Ma , Xuetai Zhu","doi":"10.1016/j.jphotobiol.2025.113338","DOIUrl":"10.1016/j.jphotobiol.2025.113338","url":null,"abstract":"<div><div>Triterpenoids from medicinal fungi have significant biological activities and industrial potential, but their low yield limits large-scale production. <em>Sanghuangporus vaninii</em>, a widely cultivated medicinal fungus, is a promising source of triterpenoids, yet the regulatory role of light-dark cycles in its triterpenoid biosynthesis remains unclear. In this study, we demonstrated that intermittent intense blue light-dark cycles (IB/D; ∼110 μmol/m<sup>2</sup>·s, 10 h/14 h) dramatically enhance triterpenoid production in <em>S. vaninii</em> MF5. IB/D treatment increased triterpenoid yield by 154 % compared to dark controls, also outperforming constant light or white light-dark regimens. Mechanistically, IB/D was associated with: (i) hyperbranching morphology (42 % reduced internode length); (ii) membrane remodeling via upregulated fatty acid desaturases and transporters, enhancing permeability 2.88-fold; and (iii) transcriptional activation of the mevalonate pathway, which was accompanied by a dramatic induction of endogenous 3-hydroxy-3-methylglutaryl-CoA synthase (<em>HMGS</em>) expression 4497-fold. Subsequent cloning and characterization of the <em>SvHMGS</em> gene-through sequence homology with known <em>HMGS</em> genes from <em>Sanghuangporus</em> species, identification of conserved HMGS domains, and prediction of its association with the mevalonic acid (MVA) pathway-suggested its potential conserved function in triterpenoid biosynthesis. This photoperiodic strategy represents a paradigm shift from constant light suppression to rhythmic induction, offering a scalable, low-cost approach to boost triterpenoid production in this medicinal fungus.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113338"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145756762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sulfonate-modified cyanine-based photoacoustic probe for selective detection of hydroxyl radicals in diabetic liver injury 一种用于糖尿病肝损伤羟基自由基选择性检测的磺胺基修饰菁基光声探针。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2026-01-01 Epub Date: 2025-11-20 DOI: 10.1016/j.jphotobiol.2025.113311

Dong-Xia Fan , Ren-Wei-Yang Zhang , Jun-Lian Liu , Lu Fan , Hong-Xia Xiang , Zhong-Yuan Cheng , Kai Wang

Hydroxyl radicals (•OH) play a critical role in oxidative stress-related diseases, yet their real-time detection in vivo remains challenging. We developed a hydroxyl radical-responsive photoacoustic probe (OHP) by modifying IR780-SO₃H with enhanced hydrophilicity (logP = −1.051) for improved biodistribution. Structural characterization confirmed the selective reduction of the conjugated system, while in vitro studies demonstrated OHP's selective and linear response to •OH with minimal interference from other ROS/RNS. In diabetic mice, OHP enabled dynamic monitoring of hepatic •OH levels, revealing elevated oxidative stress that was attenuated by metformin treatment. Ex vivo fluorescence imaging and histopathology validated the imaging results, showing strong correlation with disease severity. Biosafety assessments confirmed negligible cytotoxicity in cells and mice. OHP represents a sensitive, selective, and biocompatible tool for non-invasive •OH detection, offering potential for studying oxidative stress and therapeutic interventions.

羟基自由基（•OH）在氧化应激相关疾病中起着至关重要的作用，但它们在体内的实时检测仍然具有挑战性。我们通过修饰IR780-SO3H，增强亲水性（logP = -1.051），开发了羟基自由基响应光声探针（OHP），以改善生物分布。结构表征证实了共轭体系的选择性还原，而体外研究表明OHP对•OH具有选择性和线性响应，并且其他ROS/RNS的干扰最小。在糖尿病小鼠中，OHP能够动态监测肝脏•OH水平，揭示二甲双胍治疗后氧化应激升高。离体荧光成像和组织病理学证实了成像结果，显示与疾病严重程度有很强的相关性。生物安全评估证实，细胞和小鼠的细胞毒性可以忽略不计。OHP是一种灵敏、选择性和生物相容性的非侵入性OH检测工具，为研究氧化应激和治疗干预提供了潜力。

{"title":"A sulfonate-modified cyanine-based photoacoustic probe for selective detection of hydroxyl radicals in diabetic liver injury","authors":"Dong-Xia Fan , Ren-Wei-Yang Zhang , Jun-Lian Liu , Lu Fan , Hong-Xia Xiang , Zhong-Yuan Cheng , Kai Wang","doi":"10.1016/j.jphotobiol.2025.113311","DOIUrl":"10.1016/j.jphotobiol.2025.113311","url":null,"abstract":"<div><div>Hydroxyl radicals (•OH) play a critical role in oxidative stress-related diseases, yet their real-time detection <em>in vivo</em> remains challenging. We developed a hydroxyl radical-responsive photoacoustic probe (OHP) by modifying IR780-SO<sub>3</sub>H with enhanced hydrophilicity (logP = −1.051) for improved biodistribution. Structural characterization confirmed the selective reduction of the conjugated system, while <em>in vitro</em> studies demonstrated OHP's selective and linear response to •OH with minimal interference from other ROS/RNS. In diabetic mice, OHP enabled dynamic monitoring of hepatic •OH levels, revealing elevated oxidative stress that was attenuated by metformin treatment. <em>Ex vivo</em> fluorescence imaging and histopathology validated the imaging results, showing strong correlation with disease severity. Biosafety assessments confirmed negligible cytotoxicity in cells and mice. OHP represents a sensitive, selective, and biocompatible tool for non-invasive •OH detection, offering potential for studying oxidative stress and therapeutic interventions.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113311"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145604752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomimetic polydopamine-intercalated MgAl-layered double hydroxide for effective skin photoprotection and photodamage recovery 仿生多多巴胺嵌入镁铝层双氢氧化物，有效的皮肤光保护和光损伤恢复

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2026-01-01 Epub Date: 2025-11-22 DOI: 10.1016/j.jphotobiol.2025.113314

Pengqi Zhu , Xichen Sun , Ye Cai , Mingchen Zhao , Ruiping Zhang , Jinghua Sun

Excessive ultraviolet (UV) radiation is harmful to human health, leading to a range of skin issues including photoaging, sunburn, and skin cancer. Using sunscreen can help alleviate or provide temporary protection against the harmful effects of UV radiation. Commercial sunscreens frequently have low effectiveness and raise safety concerns. Therefore, a novel biocompatible polydopamine-intercalated MgAl-layered double hydroxides nanocomposite (PDA-LDH) was synthesized via in situ oxidation of dopamine within the interlayer of LDH at room temperature and without any additives. LDH can serve as an effective base to facilitate the formation of PDA without the need for an additional base, due to the ordered arrangement of basic hydroxyl groups on the surface of the LDH. The intercalation of PDA in the LDH interlayer ensures good biosafety, effective UV shielding, and excellent antioxidative and anti-inflammatory properties of PDA-LDH, making it suitable for skin photoprotection and the repair of photodamaged skin. PDA-LDH is poised to be a promising next-generation biomimetic sunscreen, designed to assist in the photoprotection and repair of photodamaged skin.

过度的紫外线（UV）辐射对人体健康有害，会导致一系列皮肤问题，包括光老化、晒伤和皮肤癌。使用防晒霜可以帮助减轻或提供暂时的保护，防止紫外线辐射的有害影响。商业防晒霜的功效往往很低，还会引发安全问题。因此，在室温下，不添加任何添加剂的情况下，通过在LDH层间原位氧化多巴胺，合成了一种新的生物相容性聚多巴胺-插层mgal层双氢氧化物纳米复合材料（PDA-LDH）。由于LDH表面有序排列的碱性羟基，LDH可以作为一个有效的碱，促进PDA的形成，而不需要额外的碱。PDA嵌入LDH中间层，保证了PDA-LDH良好的生物安全性、有效的紫外线屏蔽、优异的抗氧化和抗炎性能，适用于皮肤光保护和光损伤皮肤的修复。PDA-LDH是一种有前途的下一代仿生防晒霜，旨在帮助光保护和修复光损伤的皮肤。

{"title":"Biomimetic polydopamine-intercalated MgAl-layered double hydroxide for effective skin photoprotection and photodamage recovery","authors":"Pengqi Zhu , Xichen Sun , Ye Cai , Mingchen Zhao , Ruiping Zhang , Jinghua Sun","doi":"10.1016/j.jphotobiol.2025.113314","DOIUrl":"10.1016/j.jphotobiol.2025.113314","url":null,"abstract":"<div><div>Excessive ultraviolet (UV) radiation is harmful to human health, leading to a range of skin issues including photoaging, sunburn, and skin cancer. Using sunscreen can help alleviate or provide temporary protection against the harmful effects of UV radiation. Commercial sunscreens frequently have low effectiveness and raise safety concerns. Therefore, a novel biocompatible polydopamine-intercalated MgAl-layered double hydroxides nanocomposite (PDA-LDH) was synthesized via in situ oxidation of dopamine within the interlayer of LDH at room temperature and without any additives. LDH can serve as an effective base to facilitate the formation of PDA without the need for an additional base, due to the ordered arrangement of basic hydroxyl groups on the surface of the LDH. The intercalation of PDA in the LDH interlayer ensures good biosafety, effective UV shielding, and excellent antioxidative and anti-inflammatory properties of PDA-LDH, making it suitable for skin photoprotection and the repair of photodamaged skin. PDA-LDH is poised to be a promising next-generation biomimetic sunscreen, designed to assist in the photoprotection and repair of photodamaged skin.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113314"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scalable fabrication of polymeric dissolving microneedles for optimized ALA delivery in photodynamic therapy 聚合物溶解微针的可扩展制造，用于优化光动力治疗中ALA的输送

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2026-01-01 Epub Date: 2025-12-04 DOI: 10.1016/j.jphotobiol.2025.113319

Dianeth Sara Lima Bejar , Michelle Barreto Requena , Mirian Denise Stringasci , Marlon Rodrigues Garcia , Erika Toneth Ponce Ayala , Juliana Cristina Barreiro , Sebastião Pratavieira , Vanderlei Salvador Bagnato

Topical photodynamic therapy (PDT) is a minimally invasive, clinically approved treatment for non-melanoma skin cancer that relies on the conversion of photosensitizer (PS) precursors such as 5-aminolevulinic acid (ALA) into protoporphyrin IX (PpIX), followed by light activation. However, the low skin penetration of topically applied ALA cream remains a major limitation, restricting effective PpIX accumulation in deeper tumor layers. To address this challenge, we produced dissolving microneedles (DMN) as an alternative intradermal delivery platform. Two mold types were evaluated for DMN fabrication, one with a slight edge (DMNe) and another without edges (DMNf), both maintaining a conical tip geometry. DMN were prepared with a formulation containing initially 10% ALA and 20% Gantrez® AN-139 polymer in water, produced in a few steps, and characterized. In vitro insertion studies demonstrated consistent penetration depths of approximately

250 μ m

with minimal tip deformation. DMNf showed a better penetration efficiency than the DMNe and cream groups, and mass spectrometry confirmed uniform ALA distribution. In vitro assays in darkness confirmed the formulation’s biocompatibility with tumor cells. In a murine xenograft model of nodular epidermoid carcinoma, DMN-mediated ALA delivery generated up to twice the amount of PpIX in deeper tumor regions and also caused greater PDT damage compared to cream application. These findings highlight DMN as a promising approach to enhance PDT efficacy, especially for thicker or nodular skin lesions, by enabling superior and uniform intradermal drug delivery.

局部光动力疗法（PDT）是一种微创、临床批准的非黑色素瘤皮肤癌治疗方法，它依赖于光敏剂（PS）前体如5-氨基乙酰丙酸（ALA）转化为原卟啉IX (PpIX)，然后进行光激活。然而，局部应用ALA霜的皮肤渗透性低仍然是一个主要限制，限制了PpIX在更深肿瘤层的有效积累。为了解决这一挑战，我们生产了溶解微针（DMN）作为另一种皮内给药平台。评估了DMN制造的两种模具类型，一种带有轻微边缘（DMNe），另一种没有边缘（DMNf），两者都保持锥形尖端几何形状。DMN的配方最初含有10% ALA和20% Gantrez®AN-139聚合物在水中，通过几个步骤生产，并表征。体外插入研究表明，穿透深度约为250μm，尖端变形最小。DMNf的穿透效率优于DMNe组和乳膏组，质谱分析证实ALA分布均匀。体外暗室实验证实了该制剂与肿瘤细胞的生物相容性。在小鼠结节性表皮样癌异种移植模型中，dmn介导的ALA递送在更深的肿瘤区域产生的PpIX量高达两倍，并且与乳霜相比，也造成了更大的PDT损伤。这些发现突出了DMN作为一种有希望的方法来提高PDT的疗效，特别是对于较厚或结节性皮肤病变，通过实现优越和均匀的皮内给药。

{"title":"Scalable fabrication of polymeric dissolving microneedles for optimized ALA delivery in photodynamic therapy","authors":"Dianeth Sara Lima Bejar , Michelle Barreto Requena , Mirian Denise Stringasci , Marlon Rodrigues Garcia , Erika Toneth Ponce Ayala , Juliana Cristina Barreiro , Sebastião Pratavieira , Vanderlei Salvador Bagnato","doi":"10.1016/j.jphotobiol.2025.113319","DOIUrl":"10.1016/j.jphotobiol.2025.113319","url":null,"abstract":"<div><div>Topical photodynamic therapy (PDT) is a minimally invasive, clinically approved treatment for non-melanoma skin cancer that relies on the conversion of photosensitizer (PS) precursors such as 5-aminolevulinic acid (ALA) into protoporphyrin IX (PpIX), followed by light activation. However, the low skin penetration of topically applied ALA cream remains a major limitation, restricting effective PpIX accumulation in deeper tumor layers. To address this challenge, we produced dissolving microneedles (DMN) as an alternative intradermal delivery platform. Two mold types were evaluated for DMN fabrication, one with a slight edge (DMNe) and another without edges (DMNf), both maintaining a conical tip geometry. DMN were prepared with a formulation containing initially 10% ALA and 20% Gantrez® AN-139 polymer in water, produced in a few steps, and characterized. <em>In vitro</em> insertion studies demonstrated consistent penetration depths of approximately <span><math><mrow><mn>250</mn><mspace></mspace><mi>μ</mi><mi>m</mi></mrow></math></span> with minimal tip deformation. DMNf showed a better penetration efficiency than the DMNe and cream groups, and mass spectrometry confirmed uniform ALA distribution. <em>In vitro</em> assays in darkness confirmed the formulation’s biocompatibility with tumor cells. In a murine xenograft model of nodular epidermoid carcinoma, DMN-mediated ALA delivery generated up to twice the amount of PpIX in deeper tumor regions and also caused greater PDT damage compared to cream application. These findings highlight DMN as a promising approach to enhance PDT efficacy, especially for thicker or nodular skin lesions, by enabling superior and uniform intradermal drug delivery.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113319"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodamaged PSII does not accumulate in the non-appressed thylakoid membranes in the absence of PSII repair 在没有PSII修复的情况下，光损伤的PSII不会在非贴载的类囊体膜中积累

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2026-01-01 Epub Date: 2025-11-25 DOI: 10.1016/j.jphotobiol.2025.113317

Cleo Bagchus, Lennart A.I. Ramakers, Dana Verhoeven, Herbert van Amerongen, Emilie Wientjes

Photosystem II (PSII) is highly sensitive to light-induced damage. Photoinhibition, the light-dependent inactivation of PSII, is associated with an increase of excitation-energy quenching. Recovery from photoinhibition involves migration of PSII complexes from the appressed to the non-appressed region of the thylakoid membrane, where D1 (the PSII core protein most sensitive to photodamage) is degraded and repair occurs. However, it remains unclear whether damaged PSII core complexes accumulate in the stroma lamellae when repair is blocked.

Here, we combined confocal Fluorescence Lifetime Imaging Microscopy (FLIM) with biochemical fractionation of the thylakoid membrane to investigate the localization of damaged PSII following photoinhibition in the presence of lincomycin, an inhibitor of D1 synthesis. This condition mimics natural stress scenarios such as heat, where D1 synthesis is impaired.

FLIM analysis of structured, intact thylakoid membranes, segmented into grana- and stroma-lamellae-enriched regions, revealed a decrease in PSII fluorescence lifetime upon photoinhibition, consistent with increased excitation-energy quenching. Surprisingly, no significant difference in fluorescence lifetime components was observed between membrane domains, suggesting that damaged, quenched PSII does not accumulate in the stroma lamellae under these conditions. Western blot analysis of biochemically isolated membrane fractions confirmed a uniform decrease in D1 levels across grana and stroma lamellae upon photoinhibition.

Our results indicate that when D1 synthesis is blocked, the relocation and degradation of photodamaged PSII proceed efficiently enough to prevent its accumulation in the stroma lamellae. This reveals new aspects of PSII repair and demonstrates the strength of FLIM for spatially resolved analysis of the thylakoid membrane.

光系统II （PSII）对光致损伤高度敏感。光抑制，即PSII的光依赖性失活，与激发能量猝灭的增加有关。光抑制的恢复涉及PSII复合物从类囊体膜的受压区迁移到非受压区，在那里D1（对光损伤最敏感的PSII核心蛋白）被降解并进行修复。然而，当修复受阻时，受损的PSII核心复合体是否在基质层中积累尚不清楚。在这里，我们将共聚焦荧光寿命成像显微镜（FLIM）与类囊体膜的生化分离相结合，研究了在D1合成抑制剂林可霉素存在下光抑制后受损PSII的定位。这种情况模拟了自然应激情景，如高温，D1合成受损。对结构完整的类囊体膜（分为颗粒和基质片层富集区）的FLIM分析显示，光抑制后PSII荧光寿命减少，与激发能猝灭增加一致。令人惊讶的是，在膜结构域之间没有观察到荧光寿命成分的显著差异，这表明在这些条件下，受损、猝灭的PSII不会在基质层中积累。生物化学分离膜组分的Western blot分析证实，在光抑制作用下，D1水平在颗粒和基质薄片上均匀下降。我们的研究结果表明，当D1合成被阻断时，光损伤的PSII的重新定位和降解进行得足够有效，以防止其在基质片层中的积累。这揭示了PSII修复的新方面，并证明了FLIM在类囊体膜空间分辨分析中的优势。

{"title":"Photodamaged PSII does not accumulate in the non-appressed thylakoid membranes in the absence of PSII repair","authors":"Cleo Bagchus, Lennart A.I. Ramakers, Dana Verhoeven, Herbert van Amerongen, Emilie Wientjes","doi":"10.1016/j.jphotobiol.2025.113317","DOIUrl":"10.1016/j.jphotobiol.2025.113317","url":null,"abstract":"<div><div>Photosystem II (PSII) is highly sensitive to light-induced damage. Photoinhibition, the light-dependent inactivation of PSII, is associated with an increase of excitation-energy quenching. Recovery from photoinhibition involves migration of PSII complexes from the appressed to the non-appressed region of the thylakoid membrane, where D1 (the PSII core protein most sensitive to photodamage) is degraded and repair occurs. However, it remains unclear whether damaged PSII core complexes accumulate in the stroma lamellae when repair is blocked.</div><div>Here, we combined confocal Fluorescence Lifetime Imaging Microscopy (FLIM) with biochemical fractionation of the thylakoid membrane to investigate the localization of damaged PSII following photoinhibition in the presence of lincomycin, an inhibitor of D1 synthesis. This condition mimics natural stress scenarios such as heat, where D1 synthesis is impaired.</div><div>FLIM analysis of structured, intact thylakoid membranes, segmented into grana- and stroma-lamellae-enriched regions, revealed a decrease in PSII fluorescence lifetime upon photoinhibition, consistent with increased excitation-energy quenching. Surprisingly, no significant difference in fluorescence lifetime components was observed between membrane domains, suggesting that damaged, quenched PSII does not accumulate in the stroma lamellae under these conditions. Western blot analysis of biochemically isolated membrane fractions confirmed a uniform decrease in D1 levels across grana and stroma lamellae upon photoinhibition.</div><div>Our results indicate that when D1 synthesis is blocked, the relocation and degradation of photodamaged PSII proceed efficiently enough to prevent its accumulation in the stroma lamellae. This reveals new aspects of PSII repair and demonstrates the strength of FLIM for spatially resolved analysis of the thylakoid membrane.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113317"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of red light-emitting diode therapy in imiquimod-induced psoriasis in mice 红色发光二极管治疗吡喹莫德诱导的小鼠银屑病的效果。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2026-01-01 Epub Date: 2025-12-05 DOI: 10.1016/j.jphotobiol.2025.113333

Mab P. Corrêa , Rebeca D. Correia-Silva , Diego D. Santos , Nathália Rodrigues-Silva , Pâmela P. Borges , Silvana Sandri , Karin V. Greco , Cristiane D. Gil

Growing evidence supports the therapeutic potential of phototherapies, particularly light-emitting diodes (LEDs), in modulating inflammation and restoring tissue homeostasis. Here, we evaluated the effects of red LED irradiation (660 nm) in a murine model of psoriasis induced by imiquimod (IMQ). C57BL/6 mice were treated with IMQ for 12 consecutive days and exposed to LED on alternate days from day 4, totalling five sessions. Macroscopic evaluation showed a significant reduction in skin-fold thickness and Psoriasis Area and Severity Index (PASI) scores in LED-treated mice. Histological analyses confirmed that IMQ induced epidermal thickening, erythema, mast cell infiltration, and collagen alterations. LED exposure attenuated epidermal changes (p < 0.0001, IMQ + LED versus IMQ), reduced mast cell numbers (p < 0.05, IMQ + LED versus IMQ), and modulated collagen fiber distribution, although it did not reverse dermal thickening. At the systemic level, IMQ increased spleen weight and white pulp expansion, effects not prevented by LED. In skin samples, IMQ markedly elevated interleukin (IL)-1β and tumor necrosis factor (TNF)-α levels, which were significantly reduced by LED treatment (p < 0.05). In plasma, IL-22 was elevated in IMQ-treated animals but decreased in the IMQ + LED group (p < 0.05), while no significant changes were detected for IL-1β, IL-17A, IL-23, TNF-α, or macrophage inflammatory protein (MIP)-3α. Together, these results suggest that red LED phototherapy reduces clinical severity and partially modulates both local and systemic inflammation in IMQ-induced psoriasis, supporting its potential as a complementary therapeutic approach.

越来越多的证据支持光疗法，特别是发光二极管（led）在调节炎症和恢复组织稳态方面的治疗潜力。在这里，我们评估了红色LED照射（660 nm）对咪喹莫特（IMQ）诱导的银屑病小鼠模型的影响。C57BL/6小鼠IMQ连续治疗12天，从第4天开始隔天暴露于LED，共5个疗程。宏观评价显示，led治疗小鼠的皮肤褶皱厚度和银屑病面积和严重程度指数（PASI）评分显著降低。组织学分析证实IMQ诱导表皮增厚、红斑、肥大细胞浸润和胶原蛋白改变。LED照射可减弱表皮变化(p

{"title":"Effects of red light-emitting diode therapy in imiquimod-induced psoriasis in mice","authors":"Mab P. Corrêa , Rebeca D. Correia-Silva , Diego D. Santos , Nathália Rodrigues-Silva , Pâmela P. Borges , Silvana Sandri , Karin V. Greco , Cristiane D. Gil","doi":"10.1016/j.jphotobiol.2025.113333","DOIUrl":"10.1016/j.jphotobiol.2025.113333","url":null,"abstract":"<div><div>Growing evidence supports the therapeutic potential of phototherapies, particularly light-emitting diodes (LEDs), in modulating inflammation and restoring tissue homeostasis. Here, we evaluated the effects of red LED irradiation (660 nm) in a murine model of psoriasis induced by imiquimod (IMQ). C57BL/6 mice were treated with IMQ for 12 consecutive days and exposed to LED on alternate days from day 4, totalling five sessions. Macroscopic evaluation showed a significant reduction in skin-fold thickness and Psoriasis Area and Severity Index (PASI) scores in LED-treated mice. Histological analyses confirmed that IMQ induced epidermal thickening, erythema, mast cell infiltration, and collagen alterations. LED exposure attenuated epidermal changes (<em>p</em> < 0.0001, IMQ + LED versus IMQ), reduced mast cell numbers (<em>p</em> < 0.05, IMQ + LED versus IMQ), and modulated collagen fiber distribution, although it did not reverse dermal thickening. At the systemic level, IMQ increased spleen weight and white pulp expansion, effects not prevented by LED. In skin samples, IMQ markedly elevated interleukin (IL)-1β and tumor necrosis factor (TNF)-α levels, which were significantly reduced by LED treatment (<em>p</em> < 0.05). In plasma, IL-22 was elevated in IMQ-treated animals but decreased in the IMQ + LED group (p < 0.05), while no significant changes were detected for IL-1β, IL-17A, IL-23, TNF-α, or macrophage inflammatory protein (MIP)-3α. Together, these results suggest that red LED phototherapy reduces clinical severity and partially modulates both local and systemic inflammation in IMQ-induced psoriasis, supporting its potential as a complementary therapeutic approach.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113333"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145714737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gd(III) and Fe(III) ion crosslinked hyaluronic acid microgels composites embedding hetero atom doped carbon quantum dots render photodynamic therapy with improved bioimaging capability Gd（III）和Fe（III）离子交联透明质酸微凝胶复合材料包埋杂原子掺杂碳量子点，实现光动力治疗，提高生物成像能力

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2026-01-01 Epub Date: 2025-12-16 DOI: 10.1016/j.jphotobiol.2025.113342

Selin Sagbas Suner , Mehtap Sahiner , Evrim Umut , Nurettin Sahiner

In this study, we report the development of multifunctional CQ-dot@HA-Gd/Fe(III) microgels that can be readily simultaneously used in fluorescence/MR dual-mode imaging and photodynamic therapy as theragnostic agents. Nitrogen (N-) and sulfur (S-) heteroatom-doped carbon quantum dots (CQ-dot) were prepared in one step microwave treatment within 3 min as a fluorescence and photoinduced antipathogenic nanomaterial. The N/S-doped CQ-dots were spherical shaped and < 50 nm via TEM images and showed high fluorescence intensity with 420 nm emission wavelength at maximum λ_ex:350 nm. The N/S-doped CQ-dots were embedded into ionically crosslinked hyaluronic acid (HA) microgels, employing trivalent metal ions Gd(III) or Fe(III) ions. The prepared CQ-dot@HA-Gd/Fe(III) microgels <5 mm size range are injectable for possible intravenous administration and possess high fluorescent properties. The isoelectric point (IEP) of CQ-dot@HA-Gd and CQ-dot@HA-Fe(III) microgels was determined as pH 1.45. The CQ-dot@HA-Gd/Fe(III) microgels exhibit excellent hemocompatibility without causing noticeable hemolysis and blood clotting at concentrations up to 500 mg/mL. Furthermore, the toxicity of CQ-dot@HA-Gd/Fe(III) microgels on L929 fibroblast cells was found as 100 mg/mL concentration and provide brilliant cell imaging under DAPI filter without any fluorescence dye. Also, the CQ-dot@HA-Gd/Fe(III) microgel suspension afforded great MRI contrast enhancement ability. Photoinduced anticancer activity was observed for CQ-dot@HA-Gd/Fe(III) microgels even at 50 mg/mL against SK-MEL 30 melanoma cells under UV-A light treatment for 30 min. In addition, high reactive oxygen species (ROS) generation was obtained for the pathogenic bacteria cells by light-sensitive CQ-dot@HA-Gd/Fe(III) microgels upon 30 min UV-A light treatment that triggered the destruction of the Staphylococcus aureus (ATCC 6538).

在这项研究中，我们报道了多功能CQ-dot@HA-Gd/Fe（III）微凝胶的开发，该微凝胶可以很容易地同时用于荧光/磁共振双模成像和光动力治疗作为诊断剂。采用微波一步法在3 min内制备了氮（N-）和硫（S-）掺杂碳量子点（CQ-dot）作为荧光光致抗致病性纳米材料。TEM图像显示，N/ s掺杂的cq点呈球形，波长为<； 50 nm，荧光强度高，最大λex为350 nm，发射波长为420 nm。将N/ s掺杂的cq点嵌入到离子交联透明质酸（HA）微凝胶中，采用三价金属离子Gd（III）或Fe（III）离子。制备的CQ-dot@HA-Gd/Fe（III）微凝胶<； 5mm尺寸范围可注射，可能用于静脉给药，并具有高荧光特性。测定CQ-dot@HA-Gd和CQ-dot@HA-Fe（III）微凝胶的等电点（IEP） pH为1.45。CQ-dot@HA-Gd/Fe（III）微凝胶具有优异的血液相容性，浓度高达500 mg/mL时不会引起明显的溶血和凝血。此外，CQ-dot@HA-Gd/Fe（III）微凝胶在100 mg/mL浓度时对L929成纤维细胞具有毒性，在DAPI滤光片下无任何荧光染料提供了明亮的细胞成像。同时，CQ-dot@HA-Gd/Fe（III）微凝胶悬浮液具有良好的MRI增强能力。在UV-A光处理30分钟后，CQ-dot@HA-Gd/Fe（III）微凝胶对SK-MEL 30黑色素瘤细胞的光诱导抗癌活性观察到，即使浓度为50 mg/mL。此外，通过光敏CQ-dot@HA-Gd/Fe（III）微凝胶对病原菌细胞进行30min UV-A光处理，触发金黄色葡萄球菌（ATCC 6538）的破坏，获得了高活性氧（ROS）生成。

{"title":"Gd(III) and Fe(III) ion crosslinked hyaluronic acid microgels composites embedding hetero atom doped carbon quantum dots render photodynamic therapy with improved bioimaging capability","authors":"Selin Sagbas Suner , Mehtap Sahiner , Evrim Umut , Nurettin Sahiner","doi":"10.1016/j.jphotobiol.2025.113342","DOIUrl":"10.1016/j.jphotobiol.2025.113342","url":null,"abstract":"<div><div>In this study, we report the development of multifunctional CQ-dot@HA-Gd/Fe(III) microgels that can be readily simultaneously used in fluorescence/MR dual-mode imaging and photodynamic therapy as theragnostic agents. Nitrogen (N-) and sulfur (S-) heteroatom-doped carbon quantum dots (CQ-dot) were prepared in one step microwave treatment within 3 min as a fluorescence and photoinduced antipathogenic nanomaterial. The N/S-doped CQ-dots were spherical shaped and < 50 nm via TEM images and showed high fluorescence intensity with 420 nm emission wavelength at maximum λ<sub>ex</sub>:350 nm. The N/S-doped CQ-dots were embedded into ionically crosslinked hyaluronic acid (HA) microgels, employing trivalent metal ions Gd(III) or Fe(III) ions. The prepared CQ-dot@HA-Gd/Fe(III) microgels <5 mm size range are injectable for possible intravenous administration and possess high fluorescent properties. The isoelectric point (IEP) of CQ-dot@HA-Gd and CQ-dot@HA-Fe(III) microgels was determined as pH 1.45. The CQ-dot@HA-Gd/Fe(III) microgels exhibit excellent hemocompatibility without causing noticeable hemolysis and blood clotting at concentrations up to 500 mg/mL. Furthermore, the toxicity of CQ-dot@HA-Gd/Fe(III) microgels on L929 fibroblast cells was found as 100 mg/mL concentration and provide brilliant cell imaging under DAPI filter without any fluorescence dye. Also, the CQ-dot@HA-Gd/Fe(III) microgel suspension afforded great MRI contrast enhancement ability. Photoinduced anticancer activity was observed for CQ-dot@HA-Gd/Fe(III) microgels even at 50 mg/mL against SK-MEL 30 melanoma cells under UV-A light treatment for 30 min. In addition, high reactive oxygen species (ROS) generation was obtained for the pathogenic bacteria cells by light-sensitive CQ-dot@HA-Gd/Fe(III) microgels upon 30 min UV-A light treatment that triggered the destruction of the <em>Staphylococcus aureus</em> (ATCC 6538).</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113342"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic ablation of floating lung cancer cells using PVA and TPGS emulsified PLGA nanoparticles loaded with pyropheophorbide-a PVA和TPGS乳化PLGA纳米颗粒负载焦磷-a的光动力消融漂浮肺癌细胞。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2026-01-01 Epub Date: 2025-11-26 DOI: 10.1016/j.jphotobiol.2025.113318

Sasivimon Pramual , Philippe Arnoux , Kriengsak Lirdprapamongkol , Céline Frochot , Valérie Jouan-Hureaux , Muriel Barberi-Heyob , Jisnuson Svasti

Metastasis or the spread of cancer cells to other tissues is a hallmark that leads to the majority of cancer-related deaths worldwide. When metastasizing cancer cells invade into the bloodstream, they become floating cells, also known as circulating tumor cells (CTCs), which can lead to the development of metastasis-associated multidrug resistance in advanced cancer patients. Eradication of CTCs has received much attention as a strategy for preventing metastasis. Photodynamic therapy (PDT) has attracted growing interest as a minimally invasive approach for cancer treatment. Pyropheophorbide-a (PPa) is photosensitizer with advantages of relatively high wavelength absorption and high extinction coefficient; however, it has limited PDT therapeutic benefits due to poor solubility. This work aimed to employ PDT for killing CTCs by utilizing PVA and TPGS coated PLGA nanoparticles, loaded with PPa. The PPa-entrapped PLGA nanoparticles (PPa-NPs) exhibited a spherical morphology under TEM with an average size of 124.9 ± 2.3 nm and a zeta potential value of −32.0 ± 1.4 mV. The PPa-NPs enhanced singlet oxygen generation in water upon light activation. PPa-NPs successfully delivered PPa into A549 floating cells under CTC-mimicking conditions, with 21-fold increase in intracellular PPa accumulation when compared to free PPa treatment. After red light excitation, intracellular ROS level was elevated in PPa-NPs treated floating cells, in a dose-dependent manner, and correlated with photocytotoxic effect of PPa-NPs in the floating cells. Our results demonstrate that PVA and TPGS stabilized PLGA NPs efficiently preserved the photophysical properties of PPa for eradicating CTCs by PDT with red light activation.

癌细胞转移或扩散到其他组织是导致世界上大多数癌症相关死亡的一个标志。当转移性癌细胞侵入血液时，它们成为漂浮细胞，也称为循环肿瘤细胞（ctc），这可能导致晚期癌症患者发生与转移相关的多药耐药。根除ctc作为预防转移的策略已受到广泛关注。光动力疗法（PDT）作为一种微创治疗癌症的方法引起了越来越多的关注。PPa是一种波长吸收高、消光系数高的光敏剂；然而，由于溶解性差，它的PDT治疗效果有限。本工作旨在利用PVA和TPGS包被的PLGA纳米颗粒，负载PPa，利用PDT杀死ctc。在TEM下，PPa-NPs包埋的PLGA纳米颗粒呈球形，平均尺寸为124.9±2.3 nm， zeta电位值为-32.0±1.4 mV。pa - nps在光活化下增强了水中单线态氧的生成。在模拟ctc的条件下，PPa- nps成功地将PPa传递到A549漂浮细胞中，与游离PPa处理相比，细胞内PPa积累增加了21倍。红光激发后，pa - nps处理的漂浮细胞细胞内ROS水平呈剂量依赖性升高，且与pa - nps对漂浮细胞的光毒作用相关。我们的研究结果表明，PVA和TPGS稳定的PLGA NPs有效地保留了PPa的光物理性质，用于红光活化的PDT根除ctc。

{"title":"Photodynamic ablation of floating lung cancer cells using PVA and TPGS emulsified PLGA nanoparticles loaded with pyropheophorbide-a","authors":"Sasivimon Pramual , Philippe Arnoux , Kriengsak Lirdprapamongkol , Céline Frochot , Valérie Jouan-Hureaux , Muriel Barberi-Heyob , Jisnuson Svasti","doi":"10.1016/j.jphotobiol.2025.113318","DOIUrl":"10.1016/j.jphotobiol.2025.113318","url":null,"abstract":"<div><div>Metastasis or the spread of cancer cells to other tissues is a hallmark that leads to the majority of cancer-related deaths worldwide. When metastasizing cancer cells invade into the bloodstream, they become floating cells, also known as circulating tumor cells (CTCs), which can lead to the development of metastasis-associated multidrug resistance in advanced cancer patients. Eradication of CTCs has received much attention as a strategy for preventing metastasis. Photodynamic therapy (PDT) has attracted growing interest as a minimally invasive approach for cancer treatment. Pyropheophorbide-a (PPa) is photosensitizer with advantages of relatively high wavelength absorption and high extinction coefficient; however, it has limited PDT therapeutic benefits due to poor solubility. This work aimed to employ PDT for killing CTCs by utilizing PVA and TPGS coated PLGA nanoparticles, loaded with PPa. The PPa-entrapped PLGA nanoparticles (PPa-NPs) exhibited a spherical morphology under TEM with an average size of 124.9 ± 2.3 nm and a zeta potential value of −32.0 ± 1.4 mV. The PPa-NPs enhanced singlet oxygen generation in water upon light activation. PPa-NPs successfully delivered PPa into A549 floating cells under CTC-mimicking conditions, with 21-fold increase in intracellular PPa accumulation when compared to free PPa treatment. After red light excitation, intracellular ROS level was elevated in PPa-NPs treated floating cells, in a dose-dependent manner, and correlated with photocytotoxic effect of PPa-NPs in the floating cells. Our results demonstrate that PVA and TPGS stabilized PLGA NPs efficiently preserved the photophysical properties of PPa for eradicating CTCs by PDT with red light activation.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"274 ","pages":"Article 113318"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0