Journal of photochemistry and photobiology. B, Biology最新文献_第10页

Association between ultraviolet exposure and type 2 diabetes: Insights from the China Health and Retirement Longitudinal Study (CHARLS). 紫外线照射与2型糖尿病的关系：来自中国健康与退休纵向研究（CHARLS）的见解

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-01 Epub Date: 2025-08-07 DOI: 10.1016/j.jphotobiol.2025.113240

Can Ming, Zhuoyu Liu, Wei Wu, Qian Gao

Objective: To investigate the association between long-term exposure to ambient ultraviolet radiation (UVR) and the risk of developing type 2 diabetes (T2D).

Research design and methods: This study analyzed data from 14,825 participants enrolled in the 2015 China Health and Retirement Longitudinal Study (CHARLS). Ground-based daily average UVR monitoring data, collected from January 1, 2005, to December 31, 2014, across 724 stations in China, were matched to 125 cities corresponding to participants' residences. UVR exposure levels were calculated separately for annual, spring, summer, autumn, and winter. All analyses were stratified by sex. Initially, a generalized linear mixed-effects model (GLMM) was employed to assess the association between city-level UVR exposure and individual-level risk of T2D. Further stratified analyses were performed based on age, body mass index (BMI), and urban versus rural residence.

Results: Higher UVR exposure was associated with a lower risk of T2D in women (OR_annual: 0.250, 95 % CI: 0.081-0.773; OR_spring: 0.437, 95 % CI: 0.194-0.984; OR_autumn: 0.305, 95 % CI: 0.098-0.944; OR_winter: 0.227, 95 % CI: 0.079-0.655). Stratified analyses revealed that annual UVR exposure had a strong protective effect in T2D among women aged under 60 years (OR: 0.163, 95 % CI: 0.034-0.792), normal and underweight women and men (OR_women: 0.080, 95 % CI: 0.017-0.383; OR_men: 0.180, 95 % CI: 0.038-0.850) and rural women (OR: 0.207, 95 % CI: 0.065-0.661).

Conclusions: Exposure to UVR appears to confer a protective effect against T2D, with this association being especially evident in women. The protective influence is more marked among individuals younger than 60 years, those with normal weight or underweight, and those residing in rural areas.

目的：探讨长期暴露于环境紫外线辐射（UVR）与2型糖尿病（T2D）发病风险的关系。研究设计与方法：本研究分析了2015年中国健康与退休纵向研究（CHARLS）的14825名参与者的数据。2005年1月1日至2014年12月31日，中国724个站点的地面日均UVR监测数据与参与者居住地对应的125个城市相匹配。分别计算每年、春季、夏季、秋季和冬季的紫外线暴露水平。所有分析均按性别分层。首先，采用广义线性混合效应模型（GLMM）来评估城市水平UVR暴露与个体水平T2D风险之间的关系。进一步的分层分析是基于年龄、身体质量指数（BMI）和城市与农村居住。结果：较高的UVR暴露与较低的女性T2D风险相关(or - annual: 0.250, 95% CI: 0.081-0.773；ORspring: 0.437, 95% CI: 0.194-0.984；ORautumn: 0.305, 95% CI: 0.098-0.944；ORwinter: 0.227, 95% CI: 0.079-0.655)。分层分析显示，在60岁以下的女性（OR: 0.163, 95% CI: 0.034-0.792）、正常和体重过轻的女性和男性(OR: 0.080, 95% CI: 0.017-0.383；男性：0.180,95% CI: 0.038-0.850)和农村妇女（OR: 0.207, 95% CI: 0.065-0.661）。结论：暴露在紫外线下似乎对T2D具有保护作用，这种关联在女性中尤为明显。这种保护作用在60岁以下的人、体重正常或体重不足的人以及居住在农村地区的人中更为明显。

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引用次数: 0

Quinolinium-substituted porphyrins as promising antimicrobial photosensitizers 喹啉取代卟啉作为有前途的抗菌光敏剂。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-30 DOI: 10.1016/j.jphotobiol.2025.113275

Leticia D. Costa , Cátia Vieira , Mariana Q. Mesquita , Steffen Hackbarth , M. Graça P.M.S. Neves , Adelaide Almeida , Augusto C. Tomé , M. Amparo F. Faustino

Characterized by a remarkable chemical versatility and outstanding photophysical properties, porphyrins stand out as one of the most promising classes of photosensitizers for antimicrobial photodynamic therapy (aPDT). In this work, we described the synthesis, as well as the chemical and photophysical characterization of quinolinium-substituted porphyrins, and their corresponding Zn(II) complexes. The efficacy of these porphyrins in the photoinactivation of bacteria was evaluated, both alone and combined with the co-adjuvant potassium iodide, against methicillin-resistant Staphylococcus aureus (MRSA, Gram-positive) and Escherichia coli (Gram-negative) strains.

Overall, the results demonstrated the high potential of both cationic porphyrins to effectively photoinactivate bacterial strains. Cationic derivative 2, at a concentration of 1.0 μM, achieved reductions exceeding > 99.99999 % (>7.0 log₁₀ CFU mL⁻¹) for S. aureus after 10 min of white light irradiation (25 mW cm⁻²) and > 99.9 % (3.52 log₁₀ RLU) for E. coli after 60 min aPDT treatment. Similarly, the Zn(II) counterpart 2a also showed excellent efficiency, achieving ∼99.999 % reduction (4.95 log₁₀ CFU mL⁻¹) against MRSA after 60 min of aPDT at 1.0 μM, and a 3.52 log₁₀ RLU reduction for E. coli after 30 min of PDT at 5.0 μM. The action mechanism of both cationic quinolinium-substituted porphyrins demonstrated to be dependent on the singlet oxygen production, consistent with a Type II photodynamic pathway. The use of potassium iodide (KI) as co-adjuvant led to the production of reactive iodine species (RIS), namely iodine, and increased the photodynamic effect.

卟啉具有显著的化学通用性和突出的光物理性质，是抗菌光动力治疗（aPDT）中最有前途的光敏剂之一。本文介绍了喹啉取代卟啉及其相应的Zn（II）配合物的合成、化学和光物理性质。评估了这些卟啉在细菌光失活方面的效果，无论是单独使用还是与辅佐剂碘化钾联合使用，对耐甲氧西林金黄色葡萄球菌（MRSA，革兰氏阳性）和大肠杆菌（革兰氏阴性）菌株都有效。总的来说，结果表明这两种阳离子卟啉具有很高的光灭活细菌菌株的潜力。阳离子衍生物2在1.0 μM浓度下，经过10分钟白光照射（25 mW cm-2）后，金黄色葡萄球菌的还原率超过> 99.99999% (>7.0 log10 CFU mL-1)，经过60分钟aPDT处理后，大肠杆菌的还原率超过> 99.9% （3.52 log₁₀RLU）。同样，Zn（II）对应物2a也表现出优异的效率，在1.0 μM aPDT作用60分钟后，对MRSA的还原率为~ 99.999% (4.95 log10 CFU mL-1)，在5.0 μM PDT作用30分钟后，对大肠杆菌的RLU还原率为3.52 log₁₀。两种阳离子喹啉取代卟啉的作用机制均依赖于单线态产氧，符合II型光动力途径。使用碘化钾（KI）作为共佐剂导致反应性碘种（RIS）即碘的产生，并增加了光动力效应。

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引用次数: 0

The impact of near-infrared-triggered photothermal therapy on collagen organization and wound healing 近红外触发光热疗法对胶原组织和伤口愈合的影响。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-26 DOI: 10.1016/j.jphotobiol.2025.113273

Yan Chen , Chuanjie Zhang , Xiuhong Weng , Shengjun Jiang , Bo Cheng

Soft tissue wounds result in the loss of essential functions, including sensation and barrier protection. Mild photothermal therapy (PTT) has emerged as a promising therapeutic strategy for promoting wound healing and tissue regeneration, primarily through its ability to precisely regulate tissue temperature and thereby influence cellular proliferation and differentiation. In this study, gold nanorods (AuNRs) were employed as photosensitizers and co-cultured with fibroblasts to evaluate the effects of mild PTT on collagen organization and wound healing under the irradiation of 808 nm near-infrared (NIR) laser. AuNRs-mediated photothermal stimulation raised the temperature of the culture medium from 18.0 °C to 37.8 °C within 7 min. This controlled thermal stimulation significantly enhanced fibroblast proliferation and led to a more random distribution of collagen fibers. Moreover, AuNR-mediated mild PTT promoted the differentiation of fibroblast into myofibroblasts and reduced scar tissue formation. These findings highlight the therapeutic potential of mild PTT in soft tissue regeneration by modulating fibroblast behavior and remodeling collagen architecture.

软组织创伤导致基本功能的丧失，包括感觉和屏障保护。轻度光热疗法（PTT）已成为促进伤口愈合和组织再生的一种有前景的治疗策略，主要是通过其精确调节组织温度从而影响细胞增殖和分化的能力。本研究以金纳米棒（aunr）作为光敏剂，与成纤维细胞共培养，在808 nm近红外（NIR）激光照射下，研究轻度PTT对胶原组织和伤口愈合的影响。arrs介导的光热刺激在7 min内将培养基温度从18.0℃提高到37.8℃。这种受控的热刺激显著增强了成纤维细胞的增殖，并导致胶原纤维的更随机分布。此外，aunr介导的轻度PTT促进成纤维细胞向肌成纤维细胞的分化，减少疤痕组织的形成。这些发现强调了轻度PTT通过调节成纤维细胞行为和重塑胶原结构在软组织再生中的治疗潜力。

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引用次数: 0

Butyl toluidine blue as a photosensitizer for antimicrobial photodynamic therapy on titanium-associated biofilms 丁基甲苯胺蓝作为一种光敏剂用于钛相关生物膜的抗菌光动力治疗

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-26 DOI: 10.1016/j.jphotobiol.2025.113274

Amanda Paino Santana , Daniela Moreira Cunha , Rodolfo Debone Piazza , Mark Wainwright , Magda Feres , Belen Retamal-Valdes , Rafael Scaf de Molon , Valdir Gouveia Garcia , Leticia Helena Theodoro , Wirley Gonçalves Assunção , Erica Dorigatti de Avila

This in vitro study aimed to determine a safe concentration of butyl toluidine blue (BuTB) and an appropriate pre-irradiation time for antimicrobial photodynamic therapy (aPDT) to reduce polymicrobial oral biofilm without causing cytotoxicity to human cells. Titanium (Ti) discs simulating implant abutment surfaces were inoculated with saliva from patients diagnosed with peri-implantitis to create a clinically relevant polymicrobial biofilm model. Antimicrobial PDT was performed using BuTB at 0.05 mg/mL and two pre-irradiation times (1 and 5 min), followed by diode laser irradiation (InGaAlP, 660 nm, 75 mW, 60 s, 4.5 J). The antimicrobial efficacy of each protocol was evaluated, and cytotoxicity was assessed on keratinocyte (HaCaT) and human gingival fibroblast (HGF) cell lines using both quantitative and qualitative approaches. Significant bacterial reduction was observed for both 1-min (8.91 ± 0.5 log₁₀ CFU/mL; p < 0.0001) and 5-min (7.75 ± 0.6 log₁₀ CFU/mL; p < 0.0001) pre-irradiation times, compared to untreated Ti controls (12.21 ± 0.1 log₁₀ CFU/mL). Although the 5-min protocol showed slightly greater antimicrobial activity (p = 0.0076), the 1-min protocol demonstrated favorable cell viability and mitochondrial activity in both HaCaT and HGF cells. These results suggest that BuTB-mediated aPDT, at a low concentration and short pre-irradiation time, can effectively reduce polymicrobial biofilm while preserving cell viability in the conditions studied. While further studies are needed, these in vitro findings provide a valuable foundation for developing safer and more effective aPDT protocols for potential future clinical applications.

本体外研究旨在确定丁基甲苯胺蓝（butyl toluidine blue, BuTB）的安全浓度和抗菌光动力治疗（aPDT）的合适照射前时间，以减少多微生物口腔生物膜，同时又不会对人体细胞造成细胞毒性。用诊断为种植体周围炎患者的唾液接种模拟种植体基台表面的钛（Ti）圆盘，建立临床相关的多微生物生物膜模型。抗菌PDT用0.05 mg/mL的BuTB进行，两次预照射（1和5 min），然后进行二极管激光照射（InGaAlP, 660 nm, 75 mW, 60 s, 4.5 J）。采用定量和定性两种方法评估了每种方案的抗菌效果，并评估了角质形成细胞（HaCaT）和人牙龈成纤维细胞（HGF）细胞系的细胞毒性。与未处理的Ti对照组（12.21±0.1 log₁₀CFU/mL）相比，辐照前1分钟（8.91±0.5 log₁₀CFU/mL; p < 0.0001）和5分钟（7.75±0.6 log₁₀CFU/mL; p < 0.0001）的细菌数量均显著减少。虽然5分钟方案显示出稍高的抗菌活性（p = 0.0076），但1分钟方案在HaCaT和HGF细胞中均显示出良好的细胞活力和线粒体活性。这些结果表明，在研究条件下，低浓度、短预照射时间的butb介导的aPDT可以有效地减少多微生物生物膜，同时保持细胞活力。虽然还需要进一步的研究，但这些体外研究结果为开发更安全、更有效的aPDT方案提供了有价值的基础，可以用于潜在的未来临床应用。

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引用次数: 0

A theoretical model for optimizing UVA/riboflavin crosslinking 优化UVA/核黄素交联的理论模型

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-25 DOI: 10.1016/j.jphotobiol.2025.113272

Shaowu Sun , Xiaona Liu , Jiayi Dong , Xiaoyu Zhao , Chenxi Zhang , Chenyan Wang , Xiaolu Ma , Yan Wang , Xiaona Li , Zhipeng Gao , Weiyi Chen

Ultraviolet-A and riboflavin (UVA/R) crosslinking has emerged as a valuable technique for ocular disease treatments. However, the exact influence of the oxygen and riboflavin on the crosslinking has not been fully explored so far. A kinetic model was developed to predict corneal/scleral stiffening effects under varying oxygen levels, irradiation intensities, and riboflavin solution concentrations during crosslinking at a fixed fluence. The optical properties of porcine sclera were determined by UV–Vis spectrophotometry. Monte Carlo method was employed to evaluate intrastromal light absorption of riboflavin. The roles of oxygen and riboflavin in the reaction mechanism were proposed based on the kinetic interactions of reactive species within the corneal/scleral stroma. Kinetic simulations indicated that increasing intrastromal oxygen concentration, driven by higher ambient oxygen levels, significantly improves crosslinking efficiency. The crosslinker formation rate reach its maximum when the intrastromal riboflavin concentration was approximately 0.245 %. Concentrations that are either too low or too high are adversely affect the formation of crosslinker. Optimal stiffening effects could be achieved by balancing oxygen availability, irradiation intensity, and riboflavin solution concentration. The model accuracy of oxygen prediction was verified by experimental results obtained from corneal crosslinking. Linear correlations were found between the model-predicted concentration of newly induced crosslinkers and the experimentally measured mechanical properties of both the cornea and sclera under various crosslinking protocols. Our study improved the prediction model by introducing accurate optical properties of the sclera. This proposed model provides a possibility for predicting the biomechanical crosslinking efficacy of the cornea/sclera, and may be used for optimizing UVA/R crosslinking protocols in customized treatment for ocular disease.

紫外- a与核黄素（UVA/R）交联已成为一种有价值的眼部疾病治疗技术。然而，氧和核黄素对交联的确切影响至今尚未得到充分的探讨。建立了一个动力学模型来预测不同氧水平、辐照强度和核黄素溶液浓度在固定通量交联过程中的角膜/巩膜硬化效应。采用紫外可见分光光度法测定了猪巩膜的光学性质。采用蒙特卡罗方法评价核黄素的细胞内光吸收。根据角膜/巩膜基质中反应物质的动力学相互作用，提出了氧和核黄素在反应机制中的作用。动力学模拟表明，在高环境氧水平的驱动下，增加星间氧浓度显著提高交联效率。当细胞内核黄素浓度约为0.245%时，交联剂形成率达到最大。浓度过低或过高都会对交联剂的形成产生不利影响。通过平衡氧可用性、辐照强度和核黄素溶液浓度可以达到最佳的硬化效果。角膜交联实验结果验证了模型预测氧含量的准确性。在不同交联方案下，模型预测的新诱导交联剂浓度与实验测量的角膜和巩膜力学性能之间存在线性相关。我们的研究通过引入准确的巩膜光学特性来改进预测模型。该模型为预测角膜/巩膜的生物力学交联效果提供了可能，并可用于优化UVA/R交联方案，用于眼科疾病的定制治疗。

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引用次数: 0

Near-IR quantum dot (BagQD) as fluorescent sensor for live-cell imaging and in-vivo antibacterial photocatalytic therapy (APT) 近红外量子点（BagQD）作为荧光传感器用于活细胞成像和体内抗菌光催化治疗（APT）

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-24 DOI: 10.1016/j.jphotobiol.2025.113271

Subhendu Sekhar Bag , Sayantan Sinha , Aniket Banerjee , Hemanshu Mediboyana , Animes K. Golder

The development of multitasking agents that can sense, as well as damage multidrug-resistant (MDR) bacteria with no growth, is the need of the hour to overcome the increased mortality associated with antibacterial resistance. Toward this end, to overcome the limitation of organic dyes as fluorescence probes, and other developed quantum dots (QD), we present new of its kind near infrared type-I (NIR-I) CdSe/ZnSe/FeTiO₃-based perovskite quantum dot, ^BagQD, for fluorescence-guided selective bacterial imaging-sensing and permanent disinfection. ^BagQD possesses unique photodynamic and photophysical properties of the near-infrared NIR-I biological window. The excellent electronic transitions of ^BagQD resulted in efficient quantitative fluorescent sensing-imaging of gram-positive (G+ve) as well as gram-negative (G-ve) bacterial pathogens, and complete in-situ and in-vivo disinfection/elimination of pathogenic bacteria without regrowth via damaging bacterial cell wall and cellular DNA, by ROS generation during photocatalysis. The material cytotoxicity study showed no harm to the human cell line. Moreover, the therapeutic application of ^BagQD on in-vivo mice skin wound infection, revealed the generation of hair follicles and epithelization within 6–8 days. Thus, ^BagQD serves as a new alternative to antibiotics and a smart nanomedicine that can uphold the hope in revolutionizing diagnostics and clinical avenues.

为了克服与抗菌药物耐药性相关的死亡率上升，迫切需要开发能够感知并破坏未生长的多药耐药（MDR）细菌的多任务制剂。为此，为了克服有机染料作为荧光探针和其他量子点（QD）的局限性，我们提出了一种新的近红外i型（NIR-I） CdSe/ZnSe/ fetio3基钙钛矿量子点BagQD，用于荧光引导的选择性细菌成像传感和永久消毒。BagQD具有独特的近红外NIR-I生物窗口光动力学和光物理特性。BagQD优异的电子跃迁特性使其能够对革兰氏阳性（G+ve）和革兰氏阴性（G-ve）病原菌进行高效的定量荧光传感成像，并在光催化过程中产生ROS，通过破坏细菌细胞壁和细胞DNA，完成病原菌的原位和体内消毒/消除，而不再生。细胞毒性实验表明，该物质对人体细胞系无危害。此外，BagQD对小鼠皮肤伤口感染的治疗作用显示，在6-8天内毛囊和上皮的产生。因此，BagQD作为抗生素的新替代品和智能纳米药物，可以为革命性的诊断和临床途径带来希望。

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引用次数: 0

Light-activated Zein–curcumin films for the photodynamic inactivation of bacterial biofilms: Continuous vs. intermittent illumination 用于细菌生物膜光动力失活的光活化玉米素-姜黄素薄膜：连续与间歇照明

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-19 DOI: 10.1016/j.jphotobiol.2025.113270

Gabriella Nunes Ferreira , Rafael Ravagnani da Costa , Guilherme A. Paixão , Jaison M. Júnior , Thaila Q. Corrêa , Antonio S.N. de Aguiar , Alessandra R. Lima , Vanderlei S. Bagnato , Sergio A. Yoshioka , Lucas D. Dias

The development of protein-based films derived from plants and agricultural sources has gained significant attention in the medical and food industries due to their broad applicability, sustainability, low cost, and non-toxic nature. Over the years, numerous studies have reported (photo)antimicrobial films aligned with the principles of the circular economy. In this study, zein-based films immobilized with curcumin as a photosensitizer (2.5 % and 5 % w/w) were synthesized and fully characterized using UV–Vis spectroscopy, fluorescence analysis, FT-IR, thermogravimetry (TG), and water contact angle measurements. Their potential for photodynamic applications was assessed through photobleaching analysis, confirming the generation of reactive oxygen species (ROS) at both concentrations. Furthermore, microbiological evaluations were conducted against Staphylococcus aureus biofilms under two different lighting regimes: continuous and intermittent exposure at specific time intervals (0, 6, 12, 18, and 24 h). The films demonstrated promising antimicrobial activity, achieving bacterial inactivation of up to 3.5 log reductions. These findings highlight the potential applications of zein-curcumin films as coatings for medical devices (e.g., endotracheal tubes, catheters) and as sustainable food packaging materials. The incorporation of photoantimicrobial properties into biodegradable films opens new perspectives for their use in healthcare and food preservation.

基于植物和农业来源的蛋白质薄膜的开发由于其广泛的适用性、可持续性、低成本和无毒性而在医疗和食品工业中受到了极大的关注。多年来，许多研究报告（图）抗菌膜符合循环经济的原则。本研究合成了以姜黄素为光敏剂（2.5%和5% w/w）固定的玉米蛋白基薄膜，并利用紫外可见光谱、荧光分析、红外光谱、热重（TG）和水接触角测量对其进行了全面表征。通过光漂白分析评估了它们的光动力学应用潜力，确认了两种浓度下活性氧（ROS）的产生。此外，在两种不同的光照条件下，对金黄色葡萄球菌生物膜进行了微生物学评估：在特定的时间间隔（0、6、12、18和24小时）连续照射和间歇照射。该薄膜表现出了良好的抗菌活性，实现了高达3.5 log的细菌灭活。这些发现突出了玉米素-姜黄素薄膜作为医疗器械涂层（例如，气管内管，导管）和可持续食品包装材料的潜在应用。将光抗菌特性纳入可生物降解薄膜为其在医疗保健和食品保存中的应用开辟了新的前景。

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引用次数: 0

Vascular and tissue effects of photodynamic therapy in a murine cutaneous tumor model 光动力疗法对小鼠皮肤肿瘤模型血管和组织的影响

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-17 DOI: 10.1016/j.jphotobiol.2025.113269

Mirian Denise Stringasci , Michelle Barreto Requena , Clara Maria Gonçalves de Faria , Fernando Silva Ramalho , Vanderlei Salvador Bagnato

Skin cancer is the most common neoplasm worldwide and presents a significant health concern. Topical photodynamic therapy (PDT) has emerged as a therapeutic option for treating malignant and potentially precancerous skin lesions. To improve this technique and design more effective protocols, it is vital to understand the relationship between PDT damage in blood vessels and surrounding tissues. In this study, solid tumors of squamous cell carcinoma (SCC) were induced in balb/c nude mice. Damages were evaluated using histology and optical coherence tomography (OCT) images to compare PDT protocols using systemic and topical photosensitizer (PS) administration with different irradiances. The results are promising and may support the development of new and more effective PDT protocols for clinical treatment, including those that require multiple treatment sessions.

皮肤癌是世界上最常见的肿瘤，是一个重要的健康问题。局部光动力疗法（PDT）已成为治疗恶性和潜在癌前皮肤病变的一种治疗选择。为了改进这项技术并设计更有效的方案，了解血管和周围组织的PDT损伤之间的关系至关重要。本研究在balb/c裸鼠身上诱导鳞状细胞癌（SCC）实体瘤。使用组织学和光学相干断层扫描（OCT）图像对损伤进行评估，以比较使用不同辐照度的全身光敏剂和局部光敏剂（PS）的PDT方案。研究结果是有希望的，并可能支持开发新的和更有效的PDT临床治疗方案，包括那些需要多次治疗的方案。

引用次数: 0

Restoration of carious dentin using diode irradiation on unmodified and chitosan nanoparticle-modified adhesive before LED polymerization: A SEM, FTIR, and μTBS evaluation. LED聚合前未改性和壳聚糖纳米颗粒改性胶粘剂二极管辐照修复牙本质龋病：SEM、FTIR和μTBS评价。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-16 DOI: 10.1016/j.jphotobiol.2025.113265

Amer M. Alanazi , Shaheryar Shafqat , Muhammad Omar Niaz , Zainab Dua

Aim

To investigate the restoration of caries-affected dentin (CAD) using adhesive modification technique via diode laser (DL) irradiation of unmodified and modified adhesive with Chitosan nanoparticles (CHNP) before light emitting diode (LED) polymerization, and CHNPs modified adhesive+LED polymerization without DL on micro-tensile bond strength (μTBS), Degree of conversion (DC) and resin tag length (RTL).

Material and methods

Forty-four human mandibular molars were obtained, and CAD was exposed according to ICDAS criteria 5. Teeth were positioned vertically within the auto-cure acrylic resin and randomly distributed into four categories according to the adhesive modification (n = 11). Group1-Etch(E) + Bond(B) + LED,Group 2-E + B(CHNP) + LED, Group 3-E + B + DL + LED and Group 4-E + B(CHNPS) + DL + LED. All samples were restored using composite buildup followed by thermal aging. Surface characterization of CHNP with Energy-dispersive spectroscopy (EDS) was assessed along with RTL via scanning electron microscopy (SEM). μTBS and failure modes were analyzed utilizing a universal testing machine (UTM) and stereomicroscope, respectively. The DC was quantified using a Fourier Transform Infrared Spectroscopy (FTIR). A one-way analysis of variance, complemented by Tukey's post hoc test, was implemented for comparisons among the investigated groups (p < 0.05).

Results

Group 4-E + B(CHNP) + DL + LED (107.43 ± 1.55 μm) presented the longest RTL with a score of grade 3 and the highest bond strength (11.54 ± 0.37 MPa). Whereas, Group 1 (E + B+ LED) displayed the shortest RTL (76.63 ± 0.82 μm) and lowest μTBS (9.31 ± 0.17 MPa). The adhesive irradiated with DL before LED polymerization in Groups 2 and 4 presented comparable DC (p˃0.05).

Conclusion

Pre-irradiating both unmodified and modified adhesives with chitosan nanoparticles using a diode laser before LED polymerization seems to be an effective approach. This technique enhances the material's mechanical properties by improving adhesive penetration, which in turn affects the length of resin tags,μTBS, and DC.

目的：通过二极管激光（DL）照射未改性和改性壳聚糖纳米颗粒（CHNP）的胶粘剂，观察未改性和改性壳聚糖纳米颗粒（CHNP）在发光二极管（LED）聚合前修复龋病牙本质（CAD），以及CHNP改性胶粘剂+LED无DL聚合的微拉伸粘结强度（μTBS）、转化度（DC）和树脂标签长度（RTL）的变化。材料与方法：取人下颌磨牙44颗，按照ICDAS标准进行CAD暴露5。将牙齿垂直放置在自动固化丙烯酸树脂中，根据粘接剂改性随机分为四类（n = 11）。Group1-Etch (E) +债券(B) +领导,集团双电子+ B (CHNP) +领导集团3 E + B + DL +领导和集团4 E + B (CHNPS) + DL +领导。所有样品都采用复合材料堆积，然后进行热老化。利用能量色散光谱（EDS）和扫描电子显微镜（SEM）对CHNP进行了表面表征。μTBS和失效模式分别采用通用试验机（UTM）和体视显微镜进行分析。利用傅里叶变换红外光谱（FTIR）对直流电进行定量分析。结果显示，4-E + B(CHNP) + DL + LED（107.43±1.55 μm）组的RTL最长，为3级，结合强度最高（11.54±0.37 MPa）。组1 （E + B+ LED）的RTL最短（76.63±0.82 μm）， μTBS最低（9.31±0.17 MPa）。2组和4组在LED聚合前用DL辐照后的DC值相当（p < 0.05）。结论：在LED聚合前，用二极管激光对未改性和改性的壳聚糖纳米颗粒进行预照射是一种有效的方法。该技术通过提高胶粘剂的渗透性来提高材料的机械性能，从而影响树脂标签、μTBS和DC的长度。

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引用次数: 0

Synthesis of new chlorin e6 derivatives bearing galactose moieties on the macrocyclic periphery and their structure–activity relationships 含半乳糖大环的氯代e6衍生物的合成及其构效关系。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-16 DOI: 10.1016/j.jphotobiol.2025.113268

Marina V. Mal'shakova , Nikolay D. Belykh , Lydia N. Shestakova , Natalia E. Galochkina , Artem O. Belotelov , Sergey S. Soroko , Irina V. Balalaeva , Dmitry V. Belykh , Natalia Yu. Shilyagina

Recent studies of photosensitizers have increasingly focused on improving their aqueous solubility, reducing dark toxicity and enhancing photodynamic activity. In our work, we have synthesized and characterized a series of chlorin e₆ derivatives varying in the number of galactose moieties in the molecule and way they are linked to the chlorin macrocycle. We established that the quantity and localization of galactose moieties in the macrocycle affects both dark and photoinduced cytotoxicity. All of the studied derivatives exhibited singlet oxygen quantum yield photogeneration comparable to that of the clinically approved chlorin e₆ dimeglumine (Photoditazine®) with marginal exceeding. Although the incorporation of galactose moieties to the periphery of the chlorin macrocycle led to slightly decreased photostability of resulting derivatives, it did not significantly hinder their photodynamic activity. Moreover, cellular uptake of the obtained derivatives was significantly improved compared to that of Photoditazine®. Collectively, favorable spectral, photochemical, photobiological characteristics, along with improved aqueous solubility, suggest that chlorin e₆ derivatives with peripheral galactose substituents are promising photosensitizers for clinical applications.

近年来对光敏剂的研究越来越集中在改善其水溶性、降低暗毒性和光动力活性方面。在我们的工作中，我们合成并表征了一系列氯e6衍生物，这些衍生物在分子中半乳糖部分的数量和它们与氯大环的连接方式上都有所不同。我们确定了半乳糖部分在大环中的数量和定位影响暗和光诱导的细胞毒性。所有被研究的衍生物都表现出单线态氧量子产率，与临床批准的氯e6二甲基胺（photodiazine®）的产率相当，但边际超过。虽然半乳糖部分掺入到氯大环的外围会导致衍生物的光稳定性略有下降，但不会显著阻碍其光动力活性。此外，与Photoditazine®相比，所获得的衍生物的细胞摄取显着提高。总的来说，良好的光谱，光化学，光生物学特性，以及改善的水溶性，表明具有外周半乳糖取代基的氯e6衍生物是临床应用的有前途的光敏剂。

{"title":"Synthesis of new chlorin e6 derivatives bearing galactose moieties on the macrocyclic periphery and their structure–activity relationships","authors":"Marina V. Mal'shakova , Nikolay D. Belykh , Lydia N. Shestakova , Natalia E. Galochkina , Artem O. Belotelov , Sergey S. Soroko , Irina V. Balalaeva , Dmitry V. Belykh , Natalia Yu. Shilyagina","doi":"10.1016/j.jphotobiol.2025.113268","DOIUrl":"10.1016/j.jphotobiol.2025.113268","url":null,"abstract":"<div><div>Recent studies of photosensitizers have increasingly focused on improving their aqueous solubility, reducing dark toxicity and enhancing photodynamic activity. In our work, we have synthesized and characterized a series of chlorin e<sub>6</sub> derivatives varying in the number of galactose moieties in the molecule and way they are linked to the chlorin macrocycle. We established that the quantity and localization of galactose moieties in the macrocycle affects both dark and photoinduced cytotoxicity. All of the studied derivatives exhibited singlet oxygen quantum yield photogeneration comparable to that of the clinically approved chlorin e<sub>6</sub> dimeglumine (Photoditazine®) with marginal exceeding. Although the incorporation of galactose moieties to the periphery of the chlorin macrocycle led to slightly decreased photostability of resulting derivatives, it did not significantly hinder their photodynamic activity. Moreover, cellular uptake of the obtained derivatives was significantly improved compared to that of Photoditazine®. Collectively, favorable spectral, photochemical, photobiological characteristics, along with improved aqueous solubility, suggest that chlorin e<sub>6</sub> derivatives with peripheral galactose substituents are promising photosensitizers for clinical applications.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113268"},"PeriodicalIF":3.7,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0