Journal of plant physiology最新文献

{"title":"LcMYB5, an R2R3-MYB family gene from Lonicera caerulea L., enhances drought and salt tolerance in transgenic tobacco and blue honeysuckle","authors":"Chunlin Fu ,&nbsp;Chunyang Bian ,&nbsp;Jing Chen ,&nbsp;Qian Zhang ,&nbsp;Dong Qin ,&nbsp;Jiangkuo Li ,&nbsp;Peng Zhang ,&nbsp;Junwei Huo ,&nbsp;Huixin Gang","doi":"10.1016/j.jplph.2024.154409","DOIUrl":"10.1016/j.jplph.2024.154409","url":null,"abstract":"<div><div>MYB transcription factors exert crucial functions in enhancing plant stress tolerance, which is impacted by soil drought and salinity. In our study, the R2R3-type MYB transcription factor gene <em>LcMYB5</em> from blue honeysuckle (<em>Lonicera caerulea</em> L.) was successfully cloned and identified, and confirmed its nuclear localization. <em>LcMYB5</em> overexpression was vastly enhanced drought and salt tolerance in both blue honeysuckle and tobacco seedlings. After drought stress, transgenic tobacco exhibited an average survival rate of 70.30%, while most wild-type (WT) plants perished, resulting in a survival rate of only 15.33%. Following salt stress, the average survival rate for transgenic tobacco reached 77.24%, compared to just 22.47% for WT plants. Measurements indicated, that transgenic tobacco had higher proline content than WT, as well as higher superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity. Transgenic tobacco decreased chlorophyll content less dramatically than WT tobacco, despite both tobaccos having decreased chlorophyll content. Furthermore, the level of malondialdehyde (MDA) and relative conductivity were lower in transgenic tobacco compared to WT. Furthermore, LcMYB5 overexpression significantly increased the expression levels of key genes related to drought stress (<em>NCED1</em>, <em>NCED2</em>, <em>PYL4</em>, <em>PYL8</em>, and <em>CBL1</em>) and salt stress (<em>NHX1</em>, <em>SOD</em>, <em>CAT1</em>, <em>SOS1</em>, and <em>HSP17.8</em>), thus improving transgenic tobacco's stress tolerance. Compared to WT blue honeysuckle, transiently transformed LcMYB5-expressing blue honeysuckle exhibited milder damage under stress conditions, a significant increase in chlorophyll and proline content was observed, the activities of SOD, POD and CAT were also significantly increased, the increase in MDA content and relative conductivity is relatively small. Additionally, In addition, transient expression of <em>LcMYB5</em> can also positively regulate the expression of these five key genes of drought stress and five key genes of salt stress, so as to improve the resistance of transgenic blue honeysuckle to drought and salt stress. In summary, our study reveals the important regulatory role of <em>LcMYB5</em> in plant resistance to drought and salt stress, providing theoretical support and potential application value for further improving crop stress resistance.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"304 ","pages":"Article 154409"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of ammonium absorption by ammonium-preferential cassava","authors":"Yu Wang ,&nbsp;Youquan Xia ,&nbsp;Lili You ,&nbsp;Yindi Liu ,&nbsp;Jixin Zou ,&nbsp;Qing Xie ,&nbsp;Xingyu Jiang","doi":"10.1016/j.jplph.2024.154401","DOIUrl":"10.1016/j.jplph.2024.154401","url":null,"abstract":"<div><div>Cassava plants can adapt to poor soils where most other crops are unable to grow normally, suggesting that they are able to efficiently uptake and utilize nutrient elements from the soils. However, little is known about the mechanism of nutrient efficiency in the crop. Herein, we report that cassava grows better under low concentration of mixed nitrogen sources (0.15 mM NH₄NO₃) than under normal nitrogen levels. Furthermore, a low concentration of ammonium (NH₄⁺) was sufficient for cassava plants, suggesting that cassava may efficiently absorb NH₄⁺ in the high-affinity concentration range. AMT1 transporters are involved in high-affinity NH₄⁺ uptake in plants. Four AMT1-type genes were cloned from cassava plants, and all four MeAMT1 transporters (MeAMT1; 1-MeAMT1; 3, MeAMT1; 5) were found to localize at the plasma membrane. Of them, expression of MeAMT1; 1, MeAMT1; 3 and MeAMT1; 5 restored growth of a yeast mutant strain and an <em>Arabidopsis</em> mutant line lacking primary ammonium transporters under ammonium deficiency. More interestingly, both NH₄⁺ absorption mediated by MeAMT1; 5 in transgenic yeast cells and NH₄⁺ influx at cassava roots displayed a two-phase pattern characterized by high- and low-affinity. In particular, the constant of high-affinity ammonium uptake mediated by MeAMT1; 5 is similar to the Km value of high-affinity ammonium absorption at cassava roots, but also close to the ammonium concentration of most soils, suggesting that cassava can efficiently capture low amounts of NH₄⁺ from soils via plasma membrane-bound AMT1-type ammonium transporters, allowing the crop to grow and develop very well in low-nitrogen soils.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"304 ","pages":"Article 154401"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alteration in the balance between ABA and GA signaling mediates genetic variation in induction and retention of dormancy during seed maturation in wheat","authors":"Tran-Nguyen Nguyen,&nbsp;Pham Anh Tuan,&nbsp;Deepak Sharma,&nbsp;Belay T. Ayele","doi":"10.1016/j.jplph.2024.154394","DOIUrl":"10.1016/j.jplph.2024.154394","url":null,"abstract":"<div><div>Induction and retention of dormancy are among the physiological processes that take place during seed maturation; however, the molecular mechanisms underlying these events are poorly understood in wheat. This study revealed that seed maturation in wheat is associated with decreases in abscisic acid (ABA) and gibberellin (GA) levels irrespective of dormancy level exhibited by the seeds mainly via expression of specific ABA (<em>TaCYP707A</em>1) and GA (<em>TaGA3ox2</em>, <em>TaGA2ox3</em> and <em>TaGA2ox6</em>) metabolism genes. Consistently, ABA to GA level ratio decreased during maturation in both highly dormant and low-dormant seeds with no apparent difference in the ratio of their levels between the two seed samples. Our data, however, showed a close association between the induction and retention of dormancy during seed maturation and modulation of the balance between ABA and GA signaling via expression of specific genes that acts as positive regulators seed response to ABA (<em>TaPYL5</em> and <em>TaABI5</em>) and GA (<em>TaGAMyb</em>). Consistently, the highly dormant and low-dormant seeds exhibited substantial variation in their sensitivity to ABA and GA during their maturation. The findings of this study highlight that genetic variation in induction and retention of dormancy during wheat seed maturation can be mediated by a shift in balance between seed sensitivity to ABA and GA independent of a shift in balance between their levels.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"304 ","pages":"Article 154394"},"PeriodicalIF":4.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BX517, an inhibitor of the mammalian phospholipid-dependent kinase 1 (PDK1), antagonizes sucrose-induced plant growth and represses the target of rapamycin (TOR) signaling and the cell cycle through WEE1 kinase in Arabidopsis thaliana","authors":"Dolores Vázquez-Rivera ,&nbsp;Pedro Iván Huerta-Venegas ,&nbsp;Javier Raya-González ,&nbsp;César Arturo Peña-Uribe ,&nbsp;Jesús Salvador López-Bucio ,&nbsp;Ernesto García-Pineda ,&nbsp;José López-Bucio ,&nbsp;Jesús Campos-García ,&nbsp;Homero Reyes de la Cruz","doi":"10.1016/j.jplph.2024.154386","DOIUrl":"10.1016/j.jplph.2024.154386","url":null,"abstract":"<div><div>The target of rapamycin (TOR) signaling pathway is critical for plant growth and stress adaptation through maintaining the proper balance between cell proliferation and differentiation. Here, by using BX517, an inhibitor of the mammalian phosphoinositide-dependent protein kinase 1 (PDK1), we tested the hypothesis that a plant ortholog of PDK1 could influence the TOR complex activity and its target, the S6 ribosomal protein kinase (S6K) in Arabidopsis seedlings. Through locally applying sucrose to leaves, which promotes root growth and plant biomass production via TOR signaling, we could demonstrate the opposite trend upon BX517 treatment, which antagonized sucrose-induced plant growth and overly decreased root development through inhibiting the expression of mitotic cyclins CYCB1 and CYCA3 in root meristems. Evidence was gathered that the WEE1 kinase, a master regulator of the DNA damage rescue system in meristems, operates downstream of a plant BX517 target(s). TOR protein activity and WEE1 expression were analyzed through protein blots and reporter gene activity, respectively, and their relationship with meristematic cell cycle progression was tested through genetic analyses. BX517 reduced TOR kinase activity, activated WEE1 expression in shoot, root, and lateral root meristems, and inhibited meristematic cell cycle progression in roots, suggesting that PDK1 is a critical element for plant responses to mitogenic factors through modulating TOR activity. Our data uncover a relation between a PDK1 ortholog with TOR activity and the expression of WEE1 kinase for growth and stress responses in plants.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"304 ","pages":"Article 154386"},"PeriodicalIF":4.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MdFC2, a ferrochelatase gene, is a positive regulator of ALA-induced anthocyanin accumulation in apples","authors":"Yifan Yin,&nbsp;Liuzi Zhang,&nbsp;Jiangting Zhang,&nbsp;Yan Zhong,&nbsp;Liangju Wang","doi":"10.1016/j.jplph.2024.154381","DOIUrl":"10.1016/j.jplph.2024.154381","url":null,"abstract":"<div><div>5-Aminolevulinic acid (ALA), a key biosynthetic precursor of tetrapyrrole compounds, significantly induces anthocyanin accumulation in apple (<em>Malus</em> × <em>domestica</em> Borkh.) as well as other fruits. Although the molecular mechanisms of ALA-induced anthocyanin accumulation have been reported, it remains unknown whether the metabolism of ALA is involved in ALA-induced anthocyanin accumulation. Here, we found that <em>MdFC2</em>, a gene encoding ferrochelatase (MdFC2), which catalyzes the generation of heme from protoporphyrin lX (PPIX), may play an important role in ALA-induced apple anthocyanin accumulation. Exogenous ALA induced the <em>MdFC2</em> expression as well as anthocyanin accumulation in apple leaves, calli, and isolated fruits. <em>MdFC2</em> overexpression in apple leaves or calli significantly enhanced anthocyanin accumulation as well as the expression of genes involved in anthocyanin biosynthesis, while RNA interference <em>MdFC2</em> inhibited anthocyanin accumulation and the expression of genes involved in anthocyanin biosynthesis. When 2,2′-dithiodipyridine, an inhibitor of MdFC2, was added, ALA-induced anthocyanin accumulation was blocked. These results suggest that ALA-induced anthocyanin accumulation of apple may be regulated by heme or its biosynthesis, among which <em>MdFC2</em> or MdFC2 may play a critical positive regulatory role. This finding provides a novel insight to explore the mechanisms of ALA-regulating physiological processes and better application of ALA in high-quality fruit production.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"304 ","pages":"Article 154381"},"PeriodicalIF":4.0,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can the agrochemical Oryzemate treatment control the uptake of pyrene by Cucurbita pepo through the regulation of major latex-like proteins?","authors":"Natsumi Chitose ,&nbsp;Kentaro Fujita ,&nbsp;Maho Chujo ,&nbsp;Hideyuki Inui","doi":"10.1016/j.jplph.2024.154385","DOIUrl":"10.1016/j.jplph.2024.154385","url":null,"abstract":"<div><div>Members of the Cucurbitaceae family accumulate several hydrophobic organic pollutants in their above-ground parts at high concentrations. Major latex-like proteins (MLPs) identified in <em>Cucurbita pepo</em> bind to hydrophobic organic pollutants, such as pyrene and dieldrin, in roots, forming complexes that are transported via xylem vessels to the above-ground plant parts. However, soil remediation of hydrophobic organic pollutants utilizing MLPs has not been established. In this study, the uptake of the hydrophobic organic pollutant pyrene by <em>C. pepo</em> was promoted through the upregulation of the expression of <em>MLP</em> genes following agrochemical treatment. Probenazole, an active ingredient in the agrochemical Oryzemate, was previously found to upregulate the promoter activity of <em>MLP</em> genes in the roots of transgenic tobacco plants. Here, Oryzemate treatment increased the levels of MLPs in the roots and xylem sap of <em>C. pepo</em>. Oryzemate treatment slightly increased and significantly decreased the pyrene concentration in the xylem sap of <em>C. pepo</em> cultivated in high- and low-contamination soils, respectively. Probenazole competitively inhibited the binding of MLPs to pyrene <em>in vitro</em>, thereby likely suppressing its uptake by <em>C. pepo</em> in low-contamination soil. This study demonstrated that Oryzemate possesses dual effects: effective phytoremediation and safe crop production, depending on the soil contamination level.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"304 ","pages":"Article 154385"},"PeriodicalIF":4.0,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Creating of novel Wx allelic variations significantly altering Wx expression and rice eating and cooking quality","authors":"Pei Zhao ,&nbsp;Yuxia Liu ,&nbsp;Zhuyun Deng ,&nbsp;Lingtong Liu ,&nbsp;Tengwei Yu ,&nbsp;Gege Ge ,&nbsp;Bingtang Chen ,&nbsp;Tai Wang","doi":"10.1016/j.jplph.2024.154384","DOIUrl":"10.1016/j.jplph.2024.154384","url":null,"abstract":"<div><div>Granule-bound starch synthase I (GBSSI) encoding gene <em>Waxy</em> (<em>Wx</em>), which largely regulates the amylose content of rice grains, is a master module determining rice eating and cooking quality (ECQ). Fine-tuning amylose level of grains is an ideal strategy to improve rice quality. Through fine editing of <em>Wx</em>^<em>a</em> promoter and 5′UTR by CRISPR/Cas9 system, we created 14 types of novel <em>Wx</em> allelic variations, of which MT7 and MT13 were able to alter <em>Wx</em> expression and amylose content of grains. MT7 showed fragment deletion and base insertions in CAAT-boxes, hardly detectable expression levels of GBSSI mRNA and protein, and generated 5.87% amylose in grains. MT13 had fragment deletions in the A-box and the TATA-box, low expression levels of GBSSI mRNA and protein, and generated 9.61% amylose in grains. Besides of the amylose content, MT7 and MT13 significantly reduced protein content and increased lipid content of grains compared with <em>Wx</em>^<em>a</em>. A comparison of MT7, MT13 and other allelic lines demonstrated the importance of base insertion around the second CAAT-box and 31bp-deletion following the second TATA-box in modulating <em>Wx</em> expression. Thus, our study generated two novel <em>Wx</em> allelic variations which significantly alter <em>Wx</em> expression and amylose content of rice grains, providing not only new germplasms for soft rice breeding, but also insights into candidate <em>cis</em> elements of <em>Wx.</em></div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154384"},"PeriodicalIF":4.0,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Brassica napus cell number regulator 6 (BnCNR6) in Arabidopsis thaliana confers tolerance to copper","authors":"Yuanyuan Liu,&nbsp;Yuqi Song,&nbsp;Liu Shi,&nbsp;Jiaying Cao,&nbsp;Zuliang Fan,&nbsp;Wei Zhang,&nbsp;Xi Chen","doi":"10.1016/j.jplph.2024.154383","DOIUrl":"10.1016/j.jplph.2024.154383","url":null,"abstract":"<div><div>Copper is an essential but potential toxic micro-nutrient in rapeseed. So far, little is known about the mechanism of rapeseed Cu transport and detoxification. Here, we determined the function of Cu transporter, <em>Brassica napus</em> cell number regulator 6 (BnCNR6), in regulating Cu homeostasis. <em>BnCNR6</em> exhibited higher expression level in euphylla and root tips. It was found that in protoplasts and transgenic plants expressing <em>Pro35S:BnCNR6-GFP</em>, BnCNR6 was localized to the plasma membrane (PM). Expression of <em>BnCNR6</em> in the yeast (<em>Saccharomyces cerevisiae</em>), compensated the Cu hypersensitivity of <em>Δcup2</em> by promoting Cu²⁺ efflux. The overexpression of <em>BnCNR6</em> in Arabidopsis <em>athma5</em> mutant restored its growth, increased its photosynthesis, and reduced Cu²⁺ concentration in the roots. Furthermore, the roots of <em>BnCNR6</em> overexpression lines had lower net Cu influx than in those of the <em>athma5</em> mutant. These results revealed that BnCNR6 is a PM protein which is useful for detoxification to increase tolerance to Cu toxicity. Collectively, our study provides a theoretical basis for reducing Cu stress in rapeseed.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"304 ","pages":"Article 154383"},"PeriodicalIF":4.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142720780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHR1 negatively regulates nitrate reductase activity by directly inhibiting the transcription of NIA1 in Arabidopsis","authors":"Zhongjuan Liu ,&nbsp;Shaoxuan Huang ,&nbsp;Lin Zhu ,&nbsp;Chengquan Li ,&nbsp;Duanmei Zhang ,&nbsp;Mingxue Chen ,&nbsp;Yanan Liu ,&nbsp;Yongqiang Zhang","doi":"10.1016/j.jplph.2024.154382","DOIUrl":"10.1016/j.jplph.2024.154382","url":null,"abstract":"<div><div>Nitrogen (N) and phosphorus (P), as indispensable mineral elements, both play pivotal roles in plant growth and development. Despite the intimate association between nitrate signaling and inorganic phosphate (Pi) signaling, the regulatory function of Pi in N metabolism remains poorly understood. In this study, we observed that Pi deficiency leads to a reduction in the activity of nitrate reductase (NR), an essential enzyme involved in N metabolism. Furthermore, PHOSPHATE STARVATION RESPONSE 1 (PHR1), a key regulator of Pi signaling, exerts a negative impact on both NR activity and the expression of its coding gene <em>NIA1</em>. Importantly, our analysis utilizing yeast one-hybrid (Y1H) and electrophoretic mobility shift assay (EMSA) techniques reveals the direct binding of PHR1 to the <em>NIA1</em> promoter via the P1BS motifs. Subsequent transient transcription expression assay (TTEA) demonstrates PHR1 as a transcriptional suppressor of <em>NIA1</em>. In addition, it was also observed that the SPX (SYG1/Pho81/XPR1) proteins SPX1 and SPX4 can attenuate the transcriptional inhibition of <em>NIA1</em> by PHR1. Collectively, these findings reveal a mechanism through which PHR1-mediated Pi signal governs N metabolism, thus offering evidence for the precise modulation of plant growth and development via N-P interaction.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154382"},"PeriodicalIF":4.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring genetics and genomics trends to understand the link between secondary metabolic genes and agronomic traits in cereals under stress","authors":"Pooja R. Aggarwal ,&nbsp;Muthamilarasan Mehanathan ,&nbsp;Pooja Choudhary","doi":"10.1016/j.jplph.2024.154379","DOIUrl":"10.1016/j.jplph.2024.154379","url":null,"abstract":"<div><div>The plant metabolome is considered an important interface between the genome and its phenome, where it plays a significant role in regulating plant growth in response to various environmental cues. A wide array of specialized metabolites is produced by plants, which are essential for mediating environmental interactions and their adaptation. Notably, enhanced accumulation of these specialized metabolites, particularly plant secondary metabolites (PSMs), is a part of the chemical defense response that is directly linked to improved stress tolerance. Therefore, exploring the genetic diversity underlying the immense variation of the secondary metabolite pool could unravel the adaptation mechanisms in plants against different environmental stresses. The post-genomic profiling platforms have enabled the exploration of the link between metabolic diversity and important agronomic traits. The current review focuses on the major achievements and future challenges associated with plant secondary metabolite (PSM) research in graminaceous crops using advanced omics approaches. Given this, we briefly summarize different strategies adopted to explore the genetic diversity and evolution of PSMs in cereal crops. Further, we have discussed the recent technological advancements to integrate multi-omics approaches linking the metabolome diversity with the genome, transcriptome, and proteome of these crops under stress. Combining these data with phenomics (the omics of phenotypes) provides a holistic view of how plants respond to stress. Next, we outlined the genetic manipulation studies performed so far in cereals to engineer secondary metabolic pathways for enhanced stress tolerance. In summary, our review provides new insight into developing genetic and genomic trends in exploring the secondary metabolite diversity in graminaceous crops and discusses how this information can be utilized in designing strategies to generate future stress-resilient crops.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154379"},"PeriodicalIF":4.0,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}