Pub Date : 2025-02-01DOI: 10.1016/j.jplph.2024.154418
Wenxuan Wang, Haiou Wang, Xiaoyu Ren
To explore variation patterns of uptake, translocation, and accumulation processes responding to organophosphate esters (OPEs) among Poaceae plants, hydroponic and computer simulation experiments were executed. Plant growth, OPEs’ concentration, and bioinformation and transcript of lipid transporters in the three terrestrial barley, wheat, and maize and aquatic rice seedlings were studied after exposure to seven OPE congeners. Four types of plants could accumulate seven OPE congeners. OPEs could promote rice growth by upregulating IAA27 hormone gene. However, maize growth was inhibited due to upregulating IAA17 hormone gene. In general, OPEs with log Kow > 4 tended to accumulate in roots of the four types of plants. Furthermore, the uptake, translocation, and accumulation mechanism of OPEs in different plants showed species-specific, depending on chemical properties of OPEs and biological factors specifically referring to the binding ability and gene expression of lipid transporters. The uptake and accumulation of OPE in aquatic rice roots were mainly influenced by biological factors. On the contrary, terrestrial plants relied on log Kow more than biological factors. Meanwhile, TIL of Poaceae plants could be a common and key protein that contributed to OPEs accumulation.
{"title":"The difference of organophosphate esters (OPEs) uptake, translocation and accumulation mechanism between four varieties in Poaceae","authors":"Wenxuan Wang, Haiou Wang, Xiaoyu Ren","doi":"10.1016/j.jplph.2024.154418","DOIUrl":"10.1016/j.jplph.2024.154418","url":null,"abstract":"<div><div>To explore variation patterns of uptake, translocation, and accumulation processes responding to organophosphate esters (OPEs) among Poaceae plants, hydroponic and computer simulation experiments were executed. Plant growth, OPEs’ concentration, and bioinformation and transcript of lipid transporters in the three terrestrial barley, wheat, and maize and aquatic rice seedlings were studied after exposure to seven OPE congeners. Four types of plants could accumulate seven OPE congeners. OPEs could promote rice growth by upregulating <em>IAA27</em> hormone gene. However, maize growth was inhibited due to upregulating <em>IAA17</em> hormone gene. In general, OPEs with log <em>K</em><sub><em>ow</em></sub> > 4 tended to accumulate in roots of the four types of plants. Furthermore, the uptake, translocation, and accumulation mechanism of OPEs in different plants showed species-specific, depending on chemical properties of OPEs and biological factors specifically referring to the binding ability and gene expression of lipid transporters. The uptake and accumulation of OPE in aquatic rice roots were mainly influenced by biological factors. On the contrary, terrestrial plants relied on log <em>K</em><sub><em>ow</em></sub> more than biological factors. Meanwhile, TIL of Poaceae plants could be a common and key protein that contributed to OPEs accumulation.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154418"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.jplph.2024.154416
Paul Grünhofer, Lukas Schreiber
{"title":"Corrigendum to “Cutinized and suberized barriers in leaves and roots: Similarities and differences” [J. Plant Physiol. 282 (2023) 153921]","authors":"Paul Grünhofer, Lukas Schreiber","doi":"10.1016/j.jplph.2024.154416","DOIUrl":"10.1016/j.jplph.2024.154416","url":null,"abstract":"","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154416"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.jplph.2024.154414
Hubert Matkowski, Agata Daszkowska–Golec
Currently, agriculture is facing the threat of climate change. Adaptation of plants to unfavorable growth conditions is undoubtedly a great challenge for scientists. A promising solution to this problem is priming, for which chemicals, microorganisms and phytohormones can be used. The use of priming not only affects the adaptation of plants to unfavorable environmental conditions caused by water deficiency, low temperatures, heat and soil pollution, but can also improve the quantity and quality of biomass. In this review, we focus on the role of plant phytohormones in inducing priming in crop plants. We took a closer look at hormones such as abscisic acid, salicylic acid, jasmonic acid and gibberellins. We focused not only on their physiological and morphological effects, but also on what changes at the molecular level are induced by priming with phytohormones. An interesting aspect of priming is the epigenetic changes induced by phytohormones, which influence better adaptation to unfavorable conditions, which is why we addressed this topic in this review.
{"title":"Wisdom comes after facts – An update on plants priming using phytohormones","authors":"Hubert Matkowski, Agata Daszkowska–Golec","doi":"10.1016/j.jplph.2024.154414","DOIUrl":"10.1016/j.jplph.2024.154414","url":null,"abstract":"<div><div>Currently, agriculture is facing the threat of climate change. Adaptation of plants to unfavorable growth conditions is undoubtedly a great challenge for scientists. A promising solution to this problem is priming, for which chemicals, microorganisms and phytohormones can be used. The use of priming not only affects the adaptation of plants to unfavorable environmental conditions caused by water deficiency, low temperatures, heat and soil pollution, but can also improve the quantity and quality of biomass. In this review, we focus on the role of plant phytohormones in inducing priming in crop plants. We took a closer look at hormones such as abscisic acid, salicylic acid, jasmonic acid and gibberellins. We focused not only on their physiological and morphological effects, but also on what changes at the molecular level are induced by priming with phytohormones. An interesting aspect of priming is the epigenetic changes induced by phytohormones, which influence better adaptation to unfavorable conditions, which is why we addressed this topic in this review.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154414"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.jplph.2024.154415
Qing Wang , Da-Ru Wang , Xin Liu , Guo-Lin Chen, He-Dan Li, Wen-Long Ji, Man-Shu Qu, Rui Yang, Chun-Xiang You
Plants are vulnerable to various abiotic stresses in the natural growing environment, among which salt stress can seriously affect plant growth, development and yield. Protein families containing trimeric tetrapeptide repeat sequences have a crucial function in plant resilience to non-living factors and participate in multiple aspects of plant growth and development. For this investigation, we acquired the apple MdTPR16 gene. The research demonstrated that ectopic expression of MdTPR16 in Arabidopsis resulted in increased resistance to salt stress. This was observed by a drop in malondialdehyde (MDA) levels and a reduction in the buildup of reactive oxygen species (ROS) under salt stress conditions. Meanwhile, apple calli, apple seedlings and apple rooting seedlings overexpressing MdTPR16 showed reduced sensitivity to salt stress. The results indicate that MdTPR16 has a critical positive regulatory function under salt stress, which may lay the foundation for a deeper understanding of the molecular pathways of salt tolerance in apple.
{"title":"Trimeric tetrapeptide repeat protein TPR16 positively regulates salt stress in apple","authors":"Qing Wang , Da-Ru Wang , Xin Liu , Guo-Lin Chen, He-Dan Li, Wen-Long Ji, Man-Shu Qu, Rui Yang, Chun-Xiang You","doi":"10.1016/j.jplph.2024.154415","DOIUrl":"10.1016/j.jplph.2024.154415","url":null,"abstract":"<div><div>Plants are vulnerable to various abiotic stresses in the natural growing environment, among which salt stress can seriously affect plant growth, development and yield. Protein families containing trimeric tetrapeptide repeat sequences have a crucial function in plant resilience to non-living factors and participate in multiple aspects of plant growth and development. For this investigation, we acquired the apple <em>MdTPR16</em> gene. The research demonstrated that ectopic expression of <em>MdTPR16</em> in <em>Arabidopsis</em> resulted in increased resistance to salt stress. This was observed by a drop in malondialdehyde (MDA) levels and a reduction in the buildup of reactive oxygen species (ROS) under salt stress conditions. Meanwhile, apple calli, apple seedlings and apple rooting seedlings overexpressing <em>MdTPR16</em> showed reduced sensitivity to salt stress. The results indicate that <em>MdTPR16</em> has a critical positive regulatory function under salt stress, which may lay the foundation for a deeper understanding of the molecular pathways of salt tolerance in apple.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154415"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.jplph.2025.154421
Ji-Hyun Kim , Ye-Jin Son , Eui-Jung Kim , Ki-Hong Jung , Yu-Jin Kim
Pollen tubes are crucial for angiosperm plants, as they deliver sperm gametes for the essential process of double fertilization. Understanding the molecular mechanisms behind pollen tube germination and growth is critical; however, these processes remain partially elucidated in monocot cereal crops. Rapid Alkalinization Factor (RALF), a small peptide of about 5 kDa, binds to the CrRLK1L receptor and plays a role in various plant physiological processes, including reproduction and tip growth. Recently, we reported that OsRALF17 and OsRALF19 binds to the OsMTD2, pollen specific CrRLK1L member, and regulates pollen tube growth. In this study, we demonstrate that Ruptured Pollen tube (RUPO), another CrRLK1L member, is also a putative receptor for OsRALF17 and OsRALF19, and propose the formation of a receptor complex with OsMTD2. In tobacco epidermal cells, OsMTD2 and RUPO were co-localized at both the plasma membrane (PM) and the nuclear membrane. Additionally, we generated a RUPO-tagged line driven by its native promoter to visualize subcellular localization during pollen tube growth. RUPO localizes a tip-enriched distribution, with intense fluorescence at the tip's PM and cytoplasm in pollen tube. Upon treatment with synthetic OsRALF17M and OsRALF19M peptides, a reduction in the signal near the PM was observed, suggesting a potential response to these peptides. Our data support the role of RUPO as a candidate receptor for OsRALF17 and OsRALF19 in rice pollen tubes, thereby suggesting a novel mechanism for these RALFs in regulating pollen tube function. Additionally, we observed a significant delay in pollen tube burst time upon treatment with synthetic OsRALF17M and synthetic OsRALF19M. We propose that investigating this phenomenon may provide further insights into the specific signaling pathways mediated by these RALFs.
{"title":"Pollen tube-expressed RUPO forms a complex with OsMTD2 and OsRALF17 and OsRALF19 peptides in rice (Oryza sativa)","authors":"Ji-Hyun Kim , Ye-Jin Son , Eui-Jung Kim , Ki-Hong Jung , Yu-Jin Kim","doi":"10.1016/j.jplph.2025.154421","DOIUrl":"10.1016/j.jplph.2025.154421","url":null,"abstract":"<div><div>Pollen tubes are crucial for angiosperm plants, as they deliver sperm gametes for the essential process of double fertilization. Understanding the molecular mechanisms behind pollen tube germination and growth is critical; however, these processes remain partially elucidated in monocot cereal crops. Rapid Alkalinization Factor (RALF), a small peptide of about 5 kDa, binds to the CrRLK1L receptor and plays a role in various plant physiological processes, including reproduction and tip growth. Recently, we reported that OsRALF17 and OsRALF19 binds to the OsMTD2, pollen specific CrRLK1L member, and regulates pollen tube growth. In this study, we demonstrate that <em>Ruptured Pollen tube (RUPO</em>), another CrRLK1L member, is also a putative receptor for OsRALF17 and OsRALF19, and propose the formation of a receptor complex with OsMTD2. In tobacco epidermal cells, OsMTD2 and RUPO were co-localized at both the plasma membrane (PM) and the nuclear membrane. Additionally, we generated a RUPO-tagged line driven by its native promoter to visualize subcellular localization during pollen tube growth. RUPO localizes a tip-enriched distribution, with intense fluorescence at the tip's PM and cytoplasm in pollen tube. Upon treatment with synthetic OsRALF17M and OsRALF19M peptides, a reduction in the signal near the PM was observed, suggesting a potential response to these peptides. Our data support the role of RUPO as a candidate receptor for OsRALF17 and OsRALF19 in rice pollen tubes, thereby suggesting a novel mechanism for these RALFs in regulating pollen tube function. Additionally, we observed a significant delay in pollen tube burst time upon treatment with synthetic OsRALF17M and synthetic OsRALF19M. We propose that investigating this phenomenon may provide further insights into the specific signaling pathways mediated by these RALFs.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154421"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As our planet faces increasing environmental challenges, such as biotic pressures, abiotic stressors, and climate change, it is crucial to understand the complex mechanisms that underlie stress responses in crop plants. Over past few years, the integration of techniques of proteomics, transcriptomics, and genomics like LC-MS, IT-MS, MALDI-MS, DIGE, ESTs, SAGE, WGS, GWAS, GBS, 2D-PAGE, CRISPR-Cas, cDNA-AFLP, HLS, HRPF, MPSS, CAGE, MAS, IEF, MudPIT, SRM/MRM, SWATH-MS, ESI have significantly enhanced our ability to comprehend the molecular pathways and regulatory networks, involved in balancing the ecosystem/ecology stress adaptation. This review offers thorough synopsis of the current research on utilizing these multi-omics methods (including metabolomics, ionomics) for battling abiotic (salinity, temperature (chilling/freezing/cold/heat), flood (hypoxia), drought, heavy metals/loids), biotic (pathogens like fungi, bacteria, virus, pests, and insects (aphids, caterpillars, moths, mites, nematodes) and climate change stress (ozone, ultraviolet radiation, green house gases, carbon dioxide). These strategies can expedite crop improvement, and act as powerful tools with high throughput and instant database generation rates. They also provide a platform for interpreting intricate, systematic signalling pathways and knowing how different environmental stimuli cause phenotypic responses at cellular and molecular level by changing the expression of stress-responsive genes like RAB18, KIN1, RD29B, OsCIPK03, OsSTL, SIAGL, bZIP, SnRK, ABF. This review discusses various case studies that exemplify the successful implementation of these omics tools to enhance stress tolerance in plants. Finally, it highlights challenges and future prospects of utilizing these approaches in combating stress, emphasizing the need for interdisciplinary collaborations and bio-technological advancements for sustainable agriculture and food security.
{"title":"Decrypting proteomics, transcriptomics, genomics, and integrated omics for augmenting the abiotic, biotic, and climate change stress resilience in plants","authors":"Rashmi Choudhary , Faheem Ahmad , Cengiz Kaya , Sudhir Kumar Upadhyay , Sowbiya Muneer , Vinod Kumar , Mukesh Meena , Haitao Liu , Hrishikesh Upadhyaya , Chandra Shekhar Seth","doi":"10.1016/j.jplph.2025.154430","DOIUrl":"10.1016/j.jplph.2025.154430","url":null,"abstract":"<div><div>As our planet faces increasing environmental challenges, such as biotic pressures, abiotic stressors, and climate change, it is crucial to understand the complex mechanisms that underlie stress responses in crop plants. Over past few years, the integration of techniques of proteomics, transcriptomics, and genomics like LC-MS, IT-MS, MALDI-MS, DIGE, ESTs, SAGE, WGS, GWAS, GBS, 2D-PAGE, CRISPR-Cas, cDNA-AFLP, HLS, HRPF, MPSS, CAGE, MAS, IEF, MudPIT, SRM/MRM, SWATH-MS, ESI have significantly enhanced our ability to comprehend the molecular pathways and regulatory networks, involved in balancing the ecosystem/ecology stress adaptation. This review offers thorough synopsis of the current research on utilizing these multi-omics methods (including metabolomics, ionomics) for battling abiotic (salinity, temperature (chilling/freezing/cold/heat), flood (hypoxia), drought, heavy metals/loids), biotic (pathogens like fungi, bacteria, virus, pests, and insects (aphids, caterpillars, moths, mites, nematodes) and climate change stress (ozone, ultraviolet radiation, green house gases, carbon dioxide). These strategies can expedite crop improvement, and act as powerful tools with high throughput and instant database generation rates. They also provide a platform for interpreting intricate, systematic signalling pathways and knowing how different environmental stimuli cause phenotypic responses at cellular and molecular level by changing the expression of stress-responsive genes like <em>RAB18, KIN1, RD29B, OsCIPK03, OsSTL</em>, <em>SIAGL, bZIP, SnRK, ABF</em>. This review discusses various case studies that exemplify the successful implementation of these omics tools to enhance stress tolerance in plants. Finally, it highlights challenges and future prospects of utilizing these approaches in combating stress, emphasizing the need for interdisciplinary collaborations and bio-technological advancements for sustainable agriculture and food security.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154430"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Physalis peruviana L. (P. peruviana) is an edible medicinal plant rich in bioactive phenolics. This study aimed to establish a hairy root (HR) culture of P. peruviana as a potential source for the synthesis of natural compounds. HRs were induced in P. peruviana using different Agrobacterium rhizogenes strains (R1601, C58C1, A4, and K599). Notably, K599 did not induce HR formation, whereas R1601, C58C1, and A4 yielded transformation frequencies of 57.78, 65.14, and 72.31%, respectively. Secondary metabolite production and antioxidant capacity were further examined in HRs induced using C58C1, R1601, and A4. It was found that A. rhizogenes R1601 induced the greatest increase (44% compared to that observed in the non-transformed culture). The methanolic extract of HRs induced by A. rhizogenes R1601 exhibited strong antioxidant capacity, with IC50 values of 1.41 mg DE/mL and 2.33 mg DE/mL for 2,2-diphenyl-1-picrylhydrazyl and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), respectively. The HR culture showed higher production of phenolic compounds and higher antioxidant capacity than the non-transformed cultures. Ultra-performance liquid chromatography time-of-flight tandem mass spectrometry was used to identify eight alkaloids, phenolics, and glycoside compounds. A. rhizogenes R1601 is emerging as a possible strain for the mass production of HR and bioactive phenolic compounds in P. peruviana.
秘鲁Physalis peruviana L. （P. peruviana）是一种富含生物活性酚类物质的可食用药用植物。本研究旨在建立一种毛状根（HR）培养物，作为合成天然化合物的潜在来源。用不同的根状农杆菌菌株（R1601、C58C1、A4和K599）诱导秘鲁假葡萄产生hr。值得注意的是，K599不诱导HR形成，而R1601、C58C1和A4的转化频率分别为57.78%、65.14%和72.31%。C58C1、R1601和A4诱导的hr进一步检测了次生代谢物的产生和抗氧化能力。结果表明，根状芽孢杆菌R1601的诱导率最高，比未转化培养的诱导率高44%。根瘤草R1601诱导的红豆醇提物具有较强的抗氧化能力，对2,2-二苯基-1-吡啶肼和2,2-氮唑-（3-乙基苯并噻唑-6-磺酸）的IC50值分别为1.41 mg DE/mL和2.33 mg DE/mL。HR培养比未转化的培养具有更高的酚类化合物产量和更高的抗氧化能力。采用超高效液相色谱-飞行时间串联质谱法鉴定了8种生物碱、酚类和苷类化合物。根际芽孢杆菌R1601正成为大量生产紫杉树中HR和生物活性酚类化合物的可能菌株。
{"title":"Effect of different Agrobacterium rhizogenes strains on hairy root induction and analysis of metabolites in Physalis peruviana L.","authors":"Yijia Zhong , Zhie Zhou , Zhongping Yin , Lu Zhang , Qingfeng Zhang , Yihai Xie , Jiguang Chen","doi":"10.1016/j.jplph.2025.154431","DOIUrl":"10.1016/j.jplph.2025.154431","url":null,"abstract":"<div><div><em>Physalis peruviana</em> L. (<em>P. peruviana</em>) is an edible medicinal plant rich in bioactive phenolics. This study aimed to establish a hairy root (HR) culture of <em>P. peruviana</em> as a potential source for the synthesis of natural compounds. HRs were induced in <em>P. peruviana</em> using different <em>Agrobacterium rhizogenes</em> strains (R1601, C58C1, A4, and K599). Notably, K599 did not induce HR formation, whereas R1601, C58C1, and A4 yielded transformation frequencies of 57.78, 65.14, and 72.31%, respectively. Secondary metabolite production and antioxidant capacity were further examined in HRs induced using C58C1, R1601, and A4. It was found that <em>A. rhizogenes</em> R1601 induced the greatest increase (44% compared to that observed in the non-transformed culture). The methanolic extract of HRs induced by <em>A. rhizogenes</em> R1601 exhibited strong antioxidant capacity, with IC<sub>50</sub> values of 1.41 mg DE/mL and 2.33 mg DE/mL for 2,2-diphenyl-1-picrylhydrazyl and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), respectively. The HR culture showed higher production of phenolic compounds and higher antioxidant capacity than the non-transformed cultures. Ultra-performance liquid chromatography time-of-flight tandem mass spectrometry was used to identify eight alkaloids, phenolics, and glycoside compounds. <em>A. rhizogenes</em> R1601 is emerging as a possible strain for the mass production of HR and bioactive phenolic compounds in <em>P. peruviana</em>.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154431"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.jplph.2025.154435
Mingjun Yu , Jiarui Jin , Xiaoli Li , Yuping Liu , Xu Su , Marcos A. Caraballo-Ortiz , Penghui Zhang , Qian Yang , Rongju Qu , Zhaxi Cairang , Xuanlin Gao , Yinghui Zheng
Rheum tanguticum, an endemic species from the Qinghai-Xizang Plateau, is a significant perennial and medicinal plant recognized for its robust resistance to abiotic stresses, including drought, cold, and salinity. To advance the understanding of stress-response mechanisms in R. tanguticum, this study aimed to establish a reliable set of housekeeping genes as references for normalizing RT-qPCR gene expression analyses. Ten candidate genes were selected based on prior studies of related species and transcriptomic data for R. tanguticum. Their expression stability was evaluated across three tissue types (leaf, stem, and root) under four abiotic stress treatments using a comprehensive suite of bioinformatics tools. Our findings revealed that RtEF1α was the most stable reference gene in salt- and drought-stressed roots and drought-stressed stems, while RtTUB was most stable in salt-stressed stems. RtSAND was identified as the most stable reference gene in salt-, drought-, heavy metal-, and hormone-stressed leaves. For heavy metal- and hormone-stressed roots and heavy metal-stressed stems, RtUBC demonstrated the highest stability, whereas RteIF6A was most stable in hormone-stressed stems. Furthermore, the utility of these reference genes was validated by assessing the expression pattern of the drought-related gene RtNAC. This study is the first report on selecting and validating reference genes of R. tanguticum under various stress conditions, which will benefit future investigations of the genomic mechanisms involved on stress resistance in this species.
{"title":"Selection and validation of reference genes for the normalization of RT-qPCR gene expression data in Rheum tanguticum (Polygonaceae) under various abiotic stresses","authors":"Mingjun Yu , Jiarui Jin , Xiaoli Li , Yuping Liu , Xu Su , Marcos A. Caraballo-Ortiz , Penghui Zhang , Qian Yang , Rongju Qu , Zhaxi Cairang , Xuanlin Gao , Yinghui Zheng","doi":"10.1016/j.jplph.2025.154435","DOIUrl":"10.1016/j.jplph.2025.154435","url":null,"abstract":"<div><div><em>Rheum tanguticum</em>, an endemic species from the Qinghai-Xizang Plateau, is a significant perennial and medicinal plant recognized for its robust resistance to abiotic stresses, including drought, cold, and salinity. To advance the understanding of stress-response mechanisms in <em>R. tanguticum</em>, this study aimed to establish a reliable set of housekeeping genes as references for normalizing RT-qPCR gene expression analyses. Ten candidate genes were selected based on prior studies of related species and transcriptomic data for <em>R. tanguticum</em>. Their expression stability was evaluated across three tissue types (leaf, stem, and root) under four abiotic stress treatments using a comprehensive suite of bioinformatics tools. Our findings revealed that <em>RtEF1α</em> was the most stable reference gene in salt- and drought-stressed roots and drought-stressed stems, while <em>RtTUB</em> was most stable in salt-stressed stems. <em>RtSAND</em> was identified as the most stable reference gene in salt-, drought-, heavy metal-, and hormone-stressed leaves. For heavy metal- and hormone-stressed roots and heavy metal-stressed stems, <em>RtUBC</em> demonstrated the highest stability, whereas <em>RteIF6A</em> was most stable in hormone-stressed stems. Furthermore, the utility of these reference genes was validated by assessing the expression pattern of the drought-related gene <em>RtNAC</em>. This study is the first report on selecting and validating reference genes of <em>R. tanguticum</em> under various stress conditions, which will benefit future investigations of the genomic mechanisms involved on stress resistance in this species.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"305 ","pages":"Article 154435"},"PeriodicalIF":4.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-28DOI: 10.1016/j.jplph.2025.154437
Qi-Li Mi , Hong-Tao Lv , Fei-Fei Huang , Li Xu , Zi-Ying Huang , Wen-Wu Yang , Mei-Yun Zou , Hai-Tao Huang , Wan-Li Zeng , Jia-Yin Zhao , Qing-Xian Chen , Yong-En Si-Tu , Hai-Ying Xiang , Yuan-Qi Jiang , Zhi-Tong Mai , Shu-Yuan Ding , Zhan-Yu Liu , Bo-Keng Hu , Liu-Hong Li , Xue-Mei Li , Qian Gao
Nitrogen is a critical nutrient for plant growth and development. While numerous studies have investigated the mechanisms by which nitrate and/or ammonium regulate plant growth, little is known about whether and how amino acids regulate plant leaf development. This study demonstrates that tobacco plants with altered expression levels of an amino acid transporter (LYSINE HISTIDINE TRANSPORTER1, NtLHT1, Ntab0818090) exhibit significant differences in leaf morphology. Knock-out mutants exhibit elongated and narrower leaves compared to wild-type plants, whereas overexpression (OE) lines display orbicular leaves. Additionally, mutant plants exhibit decreased nitrogen use efficiency (NUE) under half MS medium and delayed development under nitrogen-depleted conditions. Moreover, overexpression lines demonstrate better performance. Although the mutant does not show significant lower level of nitrate or total amino acid content in the developing leaves, its amino acid profile, particularly glutamine (Gln), is significantly altered. Supplementation with Gln in the growth medium, rather than glutamate, can restore the morphological differences observed in mutant leaves, suggesting a pivotal role of Gln in regulating leaf shape. To further elucidate the mechanisms underlying leaf shape regulation, we analyzed endogenous hormone levels and applied exogenous hormones to these lines. Our findings suggest that amino acids transported from source organs, particularly glutamine (Gln), play a key role in controlling leaf development and morphology through the modulation of multiple phytohormones, such as gibberellic acid (GA).
{"title":"A Gln alteration influences leaf morphogenesis by mediating gibberellin levels in tobacco","authors":"Qi-Li Mi , Hong-Tao Lv , Fei-Fei Huang , Li Xu , Zi-Ying Huang , Wen-Wu Yang , Mei-Yun Zou , Hai-Tao Huang , Wan-Li Zeng , Jia-Yin Zhao , Qing-Xian Chen , Yong-En Si-Tu , Hai-Ying Xiang , Yuan-Qi Jiang , Zhi-Tong Mai , Shu-Yuan Ding , Zhan-Yu Liu , Bo-Keng Hu , Liu-Hong Li , Xue-Mei Li , Qian Gao","doi":"10.1016/j.jplph.2025.154437","DOIUrl":"10.1016/j.jplph.2025.154437","url":null,"abstract":"<div><div>Nitrogen is a critical nutrient for plant growth and development. While numerous studies have investigated the mechanisms by which nitrate and/or ammonium regulate plant growth, little is known about whether and how amino acids regulate plant leaf development. This study demonstrates that tobacco plants with altered expression levels of an amino acid transporter (LYSINE HISTIDINE TRANSPORTER1, <em>NtLHT1</em>, Ntab0818090) exhibit significant differences in leaf morphology. Knock-out mutants exhibit elongated and narrower leaves compared to wild-type plants, whereas overexpression (OE) lines display orbicular leaves. Additionally, mutant plants exhibit decreased nitrogen use efficiency (NUE) under half MS medium and delayed development under nitrogen-depleted conditions. Moreover, overexpression lines demonstrate better performance. Although the mutant does not show significant lower level of nitrate or total amino acid content in the developing leaves, its amino acid profile, particularly glutamine (Gln), is significantly altered. Supplementation with Gln in the growth medium, rather than glutamate, can restore the morphological differences observed in mutant leaves, suggesting a pivotal role of Gln in regulating leaf shape. To further elucidate the mechanisms underlying leaf shape regulation, we analyzed endogenous hormone levels and applied exogenous hormones to these lines. Our findings suggest that amino acids transported from source organs, particularly glutamine (Gln), play a key role in controlling leaf development and morphology through the modulation of multiple phytohormones, such as gibberellic acid (GA).</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"306 ","pages":"Article 154437"},"PeriodicalIF":4.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-22DOI: 10.1016/j.jplph.2025.154436
Angelika Graiff , Kiara Franke , Ulf Karsten , Daniel Liesner , Francisco J.L. Gordillo , Concepción Iñiguez
Cold-temperate and Arctic hard bottom coastal ecosystems are dominated by kelp forests, which have a high biomass production and provide important ecosystem services, but are subject to change due to ocean warming. However, the photophysiological response to increasing temperature of ecologically relevant species, such as Laminaria digitata, might depend on the local thermal environment where the population has developed. Therefore, the effects of temperature on growth rate, biochemical composition, maximum quantum yield, photosynthetic quotient and carbon budget of young cultured sporophytes of Laminaria digitata from the Arctic at Spitsbergen (SPT; cultured at 4, 10 and 16 °C) and from the cold-temperate North Sea island of Helgoland (HLG; cultured at 10, 16 and 22 °C) were comparatively analyzed. Temperature significantly affected growth rates of L. digitata from SPT and HLG, with the highest rates occurring at 10 °C, but growth did not differ between both isolates neither at 10 °C nor at 16 °C. Nevertheless, maximum quantum yield and carbon fixation rate were highest at 4 °C for the Arctic and at 16 °C for the cold-temperate L. digitata. Significantly higher rates of oxygen production and carbon fixation were observed in the cold-temperate relative to the Artic L. digitata at 10 and 16 °C, respectively. Neither temperature nor biogeographic region of origin affected the photosynthetic quotient, and release rates of dissolved or particulate organic carbon. Total carbon and mannitol content were significantly higher in the Arctic compared to the cold-temperate L. digitata at 10 °C, revealing an increased accumulation of storage compounds in the high latitude L. digitata. We conclude that L. digitata from SPT and HLG differ in their sensitivity to increasing temperatures and that the Arctic population from Spitsbergen is likely to benefit from ocean warming, while the temperate population from Helgoland will be negatively affected by further increases in ambient temperature.
{"title":"Differential effects of warming on carbon budget, photosynthetic yield and biochemical composition of cold-temperate and Arctic isolates of Laminaria digitata (Phaeophyceae)","authors":"Angelika Graiff , Kiara Franke , Ulf Karsten , Daniel Liesner , Francisco J.L. Gordillo , Concepción Iñiguez","doi":"10.1016/j.jplph.2025.154436","DOIUrl":"10.1016/j.jplph.2025.154436","url":null,"abstract":"<div><div>Cold-temperate and Arctic hard bottom coastal ecosystems are dominated by kelp forests, which have a high biomass production and provide important ecosystem services, but are subject to change due to ocean warming. However, the photophysiological response to increasing temperature of ecologically relevant species, such as <em>Laminaria digitata</em>, might depend on the local thermal environment where the population has developed. Therefore, the effects of temperature on growth rate, biochemical composition, maximum quantum yield, photosynthetic quotient and carbon budget of young cultured sporophytes of <em>Laminaria digitata</em> from the Arctic at Spitsbergen (SPT; cultured at 4, 10 and 16 °C) and from the cold-temperate North Sea island of Helgoland (HLG; cultured at 10, 16 and 22 °C) were comparatively analyzed. Temperature significantly affected growth rates of <em>L. digitata</em> from SPT and HLG, with the highest rates occurring at 10 °C, but growth did not differ between both isolates neither at 10 °C nor at 16 °C. Nevertheless, maximum quantum yield and carbon fixation rate were highest at 4 °C for the Arctic and at 16 °C for the cold-temperate <em>L. digitata</em>. Significantly higher rates of oxygen production and carbon fixation were observed in the cold-temperate relative to the Artic <em>L</em>. <em>digitata</em> at 10 and 16 °C, respectively. Neither temperature nor biogeographic region of origin affected the photosynthetic quotient, and release rates of dissolved or particulate organic carbon. Total carbon and mannitol content were significantly higher in the Arctic compared to the cold-temperate <em>L. digitata</em> at 10 °C, revealing an increased accumulation of storage compounds in the high latitude <em>L. digitata</em>. We conclude that <em>L. digitata</em> from SPT and HLG differ in their sensitivity to increasing temperatures and that the Arctic population from Spitsbergen is likely to benefit from ocean warming, while the temperate population from Helgoland will be negatively affected by further increases in ambient temperature.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"306 ","pages":"Article 154436"},"PeriodicalIF":4.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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