Journal of plant physiology最新文献_第3页

Multi-omics profiling identifies potential biological nitrification inhibitor 1,9-decanediol biosynthesis and secretion mechanisms in diverse rice varieties 多组学分析鉴定了不同水稻品种潜在的生物硝化抑制剂1,9-十二烷二醇的生物合成和分泌机制。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-02 DOI: 10.1016/j.jplph.2026.154688

Dong-Wei Di , Chi-Shang Luan , Ming-Kun Ma , Ting Yang , Herbert J. Kronzucker , Ju Min , Yufang Lu , Xiaofei Liu , Weiming Shi

1,9-Decanediol (1,9-D) was identified as the first fatty alcohol with biological nitrification inhibition (BNI) activity in rice root exudates. In a previous study, transcriptomic and untargeted metabolomic analyses contrasted two rice varieties, suggesting metabolic pathways and associated gene families involved in 1,9-D biosynthesis and secretion. However, due to the small number of varieties and technical constraints, the pool of candidate genes remained extensive, impeding precise identification and functional validation. Here, we employ ten rice varieties with diverging root-secretion properties for integrated transcriptomic and targeted metabolomic profiling. Our results demonstrate that ammonium treatment significantly enhances 1,9-D secretion across varieties, indicating a potentially conserved regulatory mechanism in rice. Integrated analysis revealed that α-linolenic acid (LN) and linoleic acid (LA) may serve as biosynthetic precursors of 1,9-D and identified key candidate genes in LN/LA metabolism, including Os04g37430, Os04g47120, Os08g39840, Os08g39850, and Os08g08220. Furthermore, our data show that root secretion of 1,9-D is actively regulated by specific transporters, including the major facilitator superfamily (MFS) member Os11g04104, the ATP-binding cassette (ABC) transporter Os01g07870, and the multidrug and toxic compound extrusion (MATE) transporter Os10g20390. This study reveals a novel multi-gene regulatory network underlying 1,9-D synthesis and secretion, providing candidate targets for improving nitrogen-use efficiency in rice.

1,9-癸二醇（1,9- d）是水稻根系分泌物中第一个具有生物硝化抑制（BNI）活性的脂肪醇。在之前的研究中，转录组学和非靶向代谢组学分析对比了两个水稻品种，发现了参与1,9- d生物合成和分泌的代谢途径和相关基因家族。然而，由于品种较少和技术限制，候选基因池仍然广泛，阻碍了精确鉴定和功能验证。在这里，我们采用了10个具有不同根分泌特性的水稻品种进行综合转录组学和靶向代谢组学分析。我们的研究结果表明，铵处理显著提高了水稻品种间1,9- d的分泌，表明了一个潜在的保守调控机制。综合分析发现α-亚麻酸（LN）和亚油酸（LA）可能是1,9- d的生物合成前体，并鉴定出参与LN/LA代谢的关键候选基因Os04g37430、Os04g47120、Os08g39840、Os08g39850和Os08g08220。此外，我们的数据显示，1,9- d的根分泌受到特定转运体的积极调节，包括主要促进剂超家族（MFS）成员Os11g04104、atp结合盒（ABC）转运体Os01g07870和多药和有毒化合物挤出（MATE）转运体Os10g20390。该研究揭示了1,9- d合成和分泌的一个新的多基因调控网络，为提高水稻氮素利用效率提供了候选靶点。

{"title":"Multi-omics profiling identifies potential biological nitrification inhibitor 1,9-decanediol biosynthesis and secretion mechanisms in diverse rice varieties","authors":"Dong-Wei Di , Chi-Shang Luan , Ming-Kun Ma , Ting Yang , Herbert J. Kronzucker , Ju Min , Yufang Lu , Xiaofei Liu , Weiming Shi","doi":"10.1016/j.jplph.2026.154688","DOIUrl":"10.1016/j.jplph.2026.154688","url":null,"abstract":"<div><div>1,9-Decanediol (1,9-D) was identified as the first fatty alcohol with biological nitrification inhibition (BNI) activity in rice root exudates. In a previous study, transcriptomic and untargeted metabolomic analyses contrasted two rice varieties, suggesting metabolic pathways and associated gene families involved in 1,9-D biosynthesis and secretion. However, due to the small number of varieties and technical constraints, the pool of candidate genes remained extensive, impeding precise identification and functional validation. Here, we employ ten rice varieties with diverging root-secretion properties for integrated transcriptomic and targeted metabolomic profiling. Our results demonstrate that ammonium treatment significantly enhances 1,9-D secretion across varieties, indicating a potentially conserved regulatory mechanism in rice. Integrated analysis revealed that α-linolenic acid (LN) and linoleic acid (LA) may serve as biosynthetic precursors of 1,9-D and identified key candidate genes in LN/LA metabolism, including Os04g37430, Os04g47120, Os08g39840, Os08g39850, and Os08g08220. Furthermore, our data show that root secretion of 1,9-D is actively regulated by specific transporters, including the major facilitator superfamily (MFS) member Os11g04104, the ATP-binding cassette (ABC) transporter Os01g07870, and the multidrug and toxic compound extrusion (MATE) transporter Os10g20390. This study reveals a novel multi-gene regulatory network underlying 1,9-D synthesis and secretion, providing candidate targets for improving nitrogen-use efficiency in rice.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"317 ","pages":"Article 154688"},"PeriodicalIF":4.1,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145917863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localization of heterosis loci for quality traits and identification of candidate genes in Brassica napus 甘蓝型油菜品质性状杂种优势位点定位及候选基因鉴定。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-31 DOI: 10.1016/j.jplph.2025.154687

Guoqiang Zheng , Ying Wang , Jiaping Wei , Zefeng Wu , Jinxiong Wang , Qi Yang , Junmei Cui , Yan Fang , Xiaoyun Dong , Xinyi Zhang , Qian Luo , Jiayue Yang , Zigang Liu

The quality traits of rapeseed are critical genetic characteristics that determine seed value and its applications. Heterosis manifests not only in yield traits but also in quality traits. In this study, multiple interspecific and intraspecific hybrid crosses were generated to evaluate their heterosis effects. Our results demonstrated that the heterosis of oil content, oleic acid, linoleic acid, and linolenic acid was higher in the winter × spring crosses than in the winter × winter crosses, whereas the opposite was true for erucic acid and glucosinolate. Furthermore, we identified a substantial numerous SNP markers through targeted sequencing and mapped the heterosis-related loci for the corresponding traits in the F₁ population. A lot of 233, 46, 247, 192, 203, and 64 QTLs were identified as being associated with the seed traits of erucic acid, glucosinolates, oleic acid, linoleic acid, linolenic acid, oil content and their heterosis. These were consolidated into 425 consensus QTLs (cq-QTLs). By integrating RNA-seq data, we identified 10 pathways involved in lipid and pyruvate metabolism, among which 28 candidate genes were annotated. These conclusions showed that these cq-QTLs can serve as hotspots for mining regulatory genes related to the heterosis of rapeseed quality traits, laying a foundation for research on the heterosis of rapeseed quality traits.

油菜籽品质性状是决定油菜籽价值及其应用的关键遗传性状。杂种优势不仅表现在产量性状上，也表现在品质性状上。本研究通过建立多个种间和种内杂交组合来评价其杂种优势效应。结果表明，冬×春杂交中油酸、亚油酸和亚麻酸的杂种优势高于冬×冬杂交，而芥酸和硫代葡萄糖苷的杂种优势则相反。此外，我们通过靶向测序鉴定了大量SNP标记，并绘制了F1群体中相应性状的杂种优势相关位点。其中233个、46个、247个、192个、203个和64个qtl与芥酸、硫代葡萄糖苷、油酸、亚油酸、亚麻酸、含油量及其杂种优势性状相关。这些被整合成425个共识qtl （cq- qtl）。通过整合RNA-seq数据，我们确定了10个参与脂质和丙酮酸代谢的途径，其中28个候选基因被注释。综上所述，这些cq- qtl可作为油菜籽品质性状杂种优势相关调控基因挖掘的热点，为油菜籽品质性状杂种优势研究奠定基础。

{"title":"Localization of heterosis loci for quality traits and identification of candidate genes in Brassica napus","authors":"Guoqiang Zheng , Ying Wang , Jiaping Wei , Zefeng Wu , Jinxiong Wang , Qi Yang , Junmei Cui , Yan Fang , Xiaoyun Dong , Xinyi Zhang , Qian Luo , Jiayue Yang , Zigang Liu","doi":"10.1016/j.jplph.2025.154687","DOIUrl":"10.1016/j.jplph.2025.154687","url":null,"abstract":"<div><div>The quality traits of rapeseed are critical genetic characteristics that determine seed value and its applications. Heterosis manifests not only in yield traits but also in quality traits. In this study, multiple interspecific and intraspecific hybrid crosses were generated to evaluate their heterosis effects. Our results demonstrated that the heterosis of oil content, oleic acid, linoleic acid, and linolenic acid was higher in the winter × spring crosses than in the winter × winter crosses, whereas the opposite was true for erucic acid and glucosinolate. Furthermore, we identified a substantial numerous SNP markers through targeted sequencing and mapped the heterosis-related loci for the corresponding traits in the F<sub>1</sub> population. A lot of 233, 46, 247, 192, 203, and 64 QTLs were identified as being associated with the seed traits of erucic acid, glucosinolates, oleic acid, linoleic acid, linolenic acid, oil content and their heterosis. These were consolidated into 425 consensus QTLs (cq-QTLs). By integrating RNA-seq data, we identified 10 pathways involved in lipid and pyruvate metabolism, among which 28 candidate genes were annotated. These conclusions showed that these cq-QTLs can serve as hotspots for mining regulatory genes related to the heterosis of rapeseed quality traits, laying a foundation for research on the heterosis of rapeseed quality traits.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"317 ","pages":"Article 154687"},"PeriodicalIF":4.1,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145917879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of mango GF14I1 and GF14I2 promotes early flowering and enhances abiotic stress tolerance in Arabidopsis 芒果GF14I1和GF14I2的过表达促进了拟南芥的早期开花并增强了非生物胁迫的耐受性。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-30 DOI: 10.1016/j.jplph.2025.154685

Jumei Wei, Liming Xia, Tianli Guo, Yanshu Meng, Kaijiang Li, Moying Lan, Yi Nai, Wenting Wu, Shuquan Chen, Weiqiaochu He, Xinhua He, Cong Luo

14-3-3 proteins, also called G-box factor 14-3-3 homologs (GF14) or G-box regulatory factors (GRFs), are highly abundant and involved in a variety of physiological regulatory processes, especially in flowering and stress regulation. This study selected a pair of GF14 genes, MiGF14I1 and MiGF14I2, which exhibit distinct intron and exon numbers, for functional characterization. MiGF14I1 and MiGF14I2 are expressed in various mango tissues, with particularly high expression levels detected in flowers. In addition, MiGF14I1 and MiGF14I2 were significantly upregulated under low-temperature, salt, and drought treatments. The overexpression of MiGF14I1 and MiGF14I2 in Arabidopsis resulted in early flowering and upregulated the expression of the bZIP transcription factors FD (AtFD), SQUAMOSA-promoter binding protein-like (AtSPL) and APETALA1 (AtAP1) in Arabidopsis. The MiGF14I1 and MiGF14I2 overexpression lines presented significantly increased germination rates, root lengths and survival rates under stress. Compared with those in the control plants, the contents of malondialdehyde (MDA) and H₂O₂ were significantly lower, whereas the content of proline was significantly greater in the transgenic plants. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) analyses revealed that MiGF14I interacted with the flowering-related proteins MiFD and FLOWERING LOCUS T (MiFT) and with several stress-related proteins, namely, NAM/ATAF1/2/CUC2 (MiNAC7), MYB30-INTERACTING E3 LIGASE 1 (MiMIEL1) and zinc finger protein 4 (MiZFP4). Moreover, yeast three-hybrid and luciferase complementation assay (LCA) analyses revealed that MiGF14I acts as a bridge to increase the interaction of MiFT with MiFD, which may lead to the formation of the flowering activation complex (FAC) of mango. These findings suggest that the MiGF14I1 and MiGF14I2 genes may play important roles in flowering and stress response in mango.

14-3-3蛋白又称G-box因子14-3-3同源物（GF14）或G-box调控因子（GRFs），是一种丰富的蛋白质，参与多种生理调控过程，特别是在开花和胁迫调控中。本研究选择了一对具有不同内含子和外显子数量的GF14基因MiGF14I1和MiGF14I2进行功能表征。MiGF14I1和MiGF14I2在芒果的各种组织中都有表达，在花中表达水平特别高。此外，MiGF14I1和MiGF14I2在低温、盐和干旱处理下均显著上调。MiGF14I1和MiGF14I2在拟南芥中过表达导致开花提前，并上调bZIP转录因子FD （AtFD）、SQUAMOSA-promoter binding protein-like （AtSPL）和APETALA1 （AtAP1）的表达。胁迫下，MiGF14I1和MiGF14I2过表达系的发芽率、根长和成活率均显著提高。与对照植株相比，转基因植株丙二醛（MDA）和H2O2含量显著降低，脯氨酸含量显著升高。酵母双杂交（Y2H）和双分子荧光互补（BiFC）分析显示，MiGF14I与开花相关蛋白MiFD和开花位点T （MiFT）以及几种胁迫相关蛋白，即NAM/ATAF1/2/CUC2 （MiNAC7）、myb30 - interaction E3 LIGASE 1 （MiMIEL1）和锌指蛋白4 （MiZFP4）相互作用。此外，酵母三杂交和荧光素酶互补试验（LCA）分析表明，MiGF14I作为桥梁增加了MiFT与MiFD的相互作用，这可能导致芒果开花激活复合物（FAC）的形成。这些发现提示MiGF14I1和MiGF14I2基因可能在芒果开花和胁迫反应中发挥重要作用。

{"title":"Overexpression of mango GF14I1 and GF14I2 promotes early flowering and enhances abiotic stress tolerance in Arabidopsis","authors":"Jumei Wei, Liming Xia, Tianli Guo, Yanshu Meng, Kaijiang Li, Moying Lan, Yi Nai, Wenting Wu, Shuquan Chen, Weiqiaochu He, Xinhua He, Cong Luo","doi":"10.1016/j.jplph.2025.154685","DOIUrl":"10.1016/j.jplph.2025.154685","url":null,"abstract":"<div><div>14-3-3 proteins, also called G-box factor 14-3-3 homologs (GF14) or G-box regulatory factors (GRFs), are highly abundant and involved in a variety of physiological regulatory processes, especially in flowering and stress regulation. This study selected a pair of <em>GF14</em> genes, <em>MiGF14I1</em> and <em>MiGF14I2</em>, which exhibit distinct intron and exon numbers, for functional characterization. <em>MiGF14I1</em> and <em>MiGF14I2</em> are expressed in various mango tissues, with particularly high expression levels detected in flowers. In addition, <em>MiGF14I1</em> and <em>MiGF14I2</em> were significantly upregulated under low-temperature, salt, and drought treatments. The overexpression of <em>MiGF14I1</em> and <em>MiGF14I2</em> in <em>Arabidopsis</em> resulted in early flowering and upregulated the expression of the bZIP transcription factors FD (AtFD), <em>SQUAMOSA-promoter binding protein-like (AtSPL)</em> and <em>APETALA1</em> (<em>AtAP1</em>) in <em>Arabidopsis</em>. The <em>MiGF14I1</em> and <em>MiGF14I2</em> overexpression lines presented significantly increased germination rates, root lengths and survival rates under stress. Compared with those in the control plants, the contents of malondialdehyde (MDA) and H<sub>2</sub>O<sub>2</sub> were significantly lower, whereas the content of proline was significantly greater in the transgenic plants. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) analyses revealed that MiGF14I interacted with the flowering-related proteins MiFD and FLOWERING LOCUS T (MiFT) and with several stress-related proteins, namely, NAM/ATAF1/2/CUC2 (MiNAC7), MYB30-INTERACTING E3 LIGASE 1 (MiMIEL1) and zinc finger protein 4 (MiZFP4). Moreover, yeast three-hybrid and luciferase complementation assay (LCA) analyses revealed that MiGF14I acts as a bridge to increase the interaction of MiFT with MiFD, which may lead to the formation of the flowering activation complex (FAC) of mango. These findings suggest that the <em>MiGF14I1</em> and <em>MiGF14I2</em> genes may play important roles in flowering and stress response in mango.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"317 ","pages":"Article 154685"},"PeriodicalIF":4.1,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145917932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global lysine crotonylation profiling reveals metabolic and stress-responsive mechanisms in Reynoutria japonica 全球赖氨酸巴豆酰化分析揭示了日本稻的代谢和应激反应机制

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-28 DOI: 10.1016/j.jplph.2025.154686

Lei You , Peng Zhang , Hongbin Cheng , Yujie Deng , Haitang Xiong , Jumei Zhang , Zhengxiu Ye , Zezhi Zhang , Chen Li , Victor Manuel Martinez Espinosa , Chao Zhou , Lanlan Zheng , Tong Li , Yonghong Zhang

Reynoutria japonica (Huzhang), also known as Japanese knotweed, is a traditionally valued medicinal herb in Asian medicine. Historically introduced to Europe and England for ornamental purposes, it has since become widely regarded as an invasive species due to its aggressive growth and adaptability. Understanding the mechanisms underlying its robust growth and environmental adaptability is therefore of both horticultural and ecological interest. Lysine crotonylation (Kcr) is a newly discovered post-translational modification implicated in diverse biological processes, but its roles in non-histone proteins, especially within medicinal plant R. japonica, remain poorly understood. Here, we present the first comprehensive proteome-wide profiling of Kcr in R. japonica. Using high-resolution liquid chromatography–tandem mass spectrometry (LC-MS/MS) coupled with immunoaffinity enrichment, we identified 18,914 Kcr sites across 5842 proteins and characterized six conserved sequence motifs, constituting the largest plant crotonylome described to date. Functional enrichment revealed that Kcr-modified proteins are primarily associated with critical metabolic pathways, including carbon fixation, photosynthesis, fatty acid degradation, the tricarboxylic acid (TCA) cycle, and protein translation. Notably, abundant Kcr modifications were found on enzymes responsible for the biosynthesis of secondary metabolites such as resveratrol and anthraquinones. Additionally, stress-responsive changes in global Kcr modification were observed, with H2B carrying the highest number of Kcr sites and showing a marked reduction under stress. These findings provide novel insights into the functional significance of Kcr in plant metabolic regulation and stress adaptation.

虎杖，又称日本结缕草，是亚洲医学中一种传统药材。历史上，它被引入欧洲和英国作为观赏目的，由于其侵略性的生长和适应性，它被广泛认为是一种入侵物种。因此，了解其强劲生长和环境适应性的机制具有园艺和生态学的双重意义。赖氨酸巴豆酰化（Lysine crotonylation, Kcr）是一种新发现的涉及多种生物学过程的翻译后修饰，但其在非组蛋白中的作用，特别是在药用植物粳稻中的作用尚不清楚。在这里，我们提出了粳稻中Kcr的第一个全面的蛋白质组分析。利用高分辨率液相色谱-串联质谱（LC-MS/MS）结合免疫亲和富集技术，研究人员在5842个蛋白中鉴定了18914个Kcr位点，并鉴定了6个保守序列基序，构成了迄今为止所描述的最大的植物crotonyome。功能富集表明，kcr修饰蛋白主要与关键代谢途径相关，包括碳固定、光合作用、脂肪酸降解、三羧酸（TCA）循环和蛋白质翻译。值得注意的是，在负责次生代谢产物（如白藜芦醇和蒽醌）生物合成的酶上发现了大量的Kcr修饰。此外，还观察到全球Kcr修饰的应激响应变化，H2B携带的Kcr位点数量最多，并且在应激下显着减少。这些发现为Kcr在植物代谢调节和逆境适应中的功能意义提供了新的见解。

{"title":"Global lysine crotonylation profiling reveals metabolic and stress-responsive mechanisms in Reynoutria japonica","authors":"Lei You , Peng Zhang , Hongbin Cheng , Yujie Deng , Haitang Xiong , Jumei Zhang , Zhengxiu Ye , Zezhi Zhang , Chen Li , Victor Manuel Martinez Espinosa , Chao Zhou , Lanlan Zheng , Tong Li , Yonghong Zhang","doi":"10.1016/j.jplph.2025.154686","DOIUrl":"10.1016/j.jplph.2025.154686","url":null,"abstract":"<div><div><em>Reynoutria japonica</em> (Huzhang), also known as Japanese knotweed, is a traditionally valued medicinal herb in Asian medicine. Historically introduced to Europe and England for ornamental purposes, it has since become widely regarded as an invasive species due to its aggressive growth and adaptability. Understanding the mechanisms underlying its robust growth and environmental adaptability is therefore of both horticultural and ecological interest. Lysine crotonylation (Kcr) is a newly discovered post-translational modification implicated in diverse biological processes, but its roles in non-histone proteins, especially within medicinal plant <em>R. japonica</em>, remain poorly understood. Here, we present the first comprehensive proteome-wide profiling of Kcr in <em>R. japonica</em>. Using high-resolution liquid chromatography–tandem mass spectrometry (LC-MS/MS) coupled with immunoaffinity enrichment, we identified 18,914 Kcr sites across 5842 proteins and characterized six conserved sequence motifs, constituting the largest plant crotonylome described to date. Functional enrichment revealed that Kcr-modified proteins are primarily associated with critical metabolic pathways, including carbon fixation, photosynthesis, fatty acid degradation, the tricarboxylic acid (TCA) cycle, and protein translation. Notably, abundant Kcr modifications were found on enzymes responsible for the biosynthesis of secondary metabolites such as resveratrol and anthraquinones. Additionally, stress-responsive changes in global Kcr modification were observed, with H2B carrying the highest number of Kcr sites and showing a marked reduction under stress. These findings provide novel insights into the functional significance of Kcr in plant metabolic regulation and stress adaptation.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"317 ","pages":"Article 154686"},"PeriodicalIF":4.1,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into responses to elevated temperatures in Solanum tuberosum cultivars with contrasting sensitivity 洞察对高温的响应在龙葵品种与对比敏感性

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-23 DOI: 10.1016/j.jplph.2025.154683

Selina Beck , Luisa Höfner , David Rüscher , Danuše Tarkowská , Jitka Široká , Stephen Reid , David Pscheidt , Jörg Hofmann , Ondřej Novák , Miroslav Strnad , Sophia Sonnewald

Elevated temperatures caused by climate change threaten potato production. To understand heat stress adaptations and variety-specific responses, plants of a susceptible (Cecile) and a tolerant cultivar (Solara) were exposed to elevated temperatures (30/28 °C) for 21 days at tuberization stage. Phenotypic, physiological, transcriptional and metabolic changes were analyzed in comparison to ambient temperatures (21/19 °C). Heat stress caused shoot elongation and tuber weight loss, which were more pronounced in Cecile. Transcriptome analysis of leaf samples revealed a stronger decrease of photosynthesis-associated genes in the sensitive cultivar Cecile, which was associated with decreased chlorophyll fluorescence and an early senescence. These effects correlated with strongly elevated levels of salicylic acid and ethylene. In contrast, Solara showed delayed senescence and a higher expression of sugar and amino acid transporters suggesting an adaptive mechanism to maintain carbohydrate and amino acid allocation. The expression of known tuberization regulators including SP6A, exhibited a similar response to heat in both varieties, with decreasing expression of SP6A. Solara exhibited a constitutively higher expression of PEBP14/15 and MADS13, which potentially promote tuberization and may support tuber growth under heat. Regardless of variety, a few genes, such as HSP20 and HSP70, were induced by heat and may serve as heat stress marker genes. Altogether, the results indicate that delayed senescence, stable photosynthesis, efficient assimilate translocation, and differential regulation of tuberization pathways contribute to heat tolerance in Solara. These insights improve our understanding of the molecular basis of heat resilience and provide potential targets for breeding climate-resilient potato varieties.

气候变化导致的气温升高威胁着马铃薯的生产。为了了解热胁迫的适应性和品种特异性反应，将一个敏感品种（Cecile）和一个耐受性品种（Solara）的植株在结核期暴露在高温（30/28°C）下21天。与环境温度（21/19°C）相比，分析了表型、生理、转录和代谢变化。热胁迫导致了茎伸长和块茎重量下降，其中在茜草中表现得更为明显。叶片样本转录组分析显示，敏感品种茜草的光合作用相关基因明显减少，这与叶绿素荧光下降和早期衰老有关。这些影响与水杨酸和乙烯水平的升高密切相关。相比之下，Solara表现出延迟衰老和糖和氨基酸转运蛋白的更高表达，这表明一种维持碳水化合物和氨基酸分配的适应性机制。在两个品种中，包括SP6A在内的已知结核调节因子的表达对高温的响应相似，但SP6A的表达量有所下降。Solara表现出PEBP14/15和MADS13的高表达，这可能促进了块茎的形成，并可能支持块茎在高温下的生长。无论品种如何，少数基因如HSP20和HSP70被热诱导，可能作为热应激标记基因。综上所述，延缓衰老、稳定的光合作用、高效的同化物转运和不同调控的结核途径对茄的耐热性有重要影响。这些见解提高了我们对耐热性分子基础的理解，并为培育气候适应型马铃薯品种提供了潜在的目标。

{"title":"Insights into responses to elevated temperatures in Solanum tuberosum cultivars with contrasting sensitivity","authors":"Selina Beck , Luisa Höfner , David Rüscher , Danuše Tarkowská , Jitka Široká , Stephen Reid , David Pscheidt , Jörg Hofmann , Ondřej Novák , Miroslav Strnad , Sophia Sonnewald","doi":"10.1016/j.jplph.2025.154683","DOIUrl":"10.1016/j.jplph.2025.154683","url":null,"abstract":"<div><div>Elevated temperatures caused by climate change threaten potato production. To understand heat stress adaptations and variety-specific responses, plants of a susceptible (Cecile) and a tolerant cultivar (Solara) were exposed to elevated temperatures (30/28 °C) for 21 days at tuberization stage. Phenotypic, physiological, transcriptional and metabolic changes were analyzed in comparison to ambient temperatures (21/19 °C). Heat stress caused shoot elongation and tuber weight loss, which were more pronounced in Cecile. Transcriptome analysis of leaf samples revealed a stronger decrease of photosynthesis-associated genes in the sensitive cultivar Cecile, which was associated with decreased chlorophyll fluorescence and an early senescence. These effects correlated with strongly elevated levels of salicylic acid and ethylene. In contrast, Solara showed delayed senescence and a higher expression of sugar and amino acid transporters suggesting an adaptive mechanism to maintain carbohydrate and amino acid allocation. The expression of known tuberization regulators including <em>SP6A</em>, exhibited a similar response to heat in both varieties, with decreasing expression of <em>SP6A</em>. Solara exhibited a constitutively higher expression of <em>PEBP14/15</em> and <em>MADS13</em>, which potentially promote tuberization and may support tuber growth under heat. Regardless of variety, a few genes, such as <em>HSP20</em> and <em>HSP70</em>, were induced by heat and may serve as heat stress marker genes. Altogether, the results indicate that delayed senescence, stable photosynthesis, efficient assimilate translocation, and differential regulation of tuberization pathways contribute to heat tolerance in Solara. These insights improve our understanding of the molecular basis of heat resilience and provide potential targets for breeding climate-resilient potato varieties.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"317 ","pages":"Article 154683"},"PeriodicalIF":4.1,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MsTIFY10a gene from alfalfa negatively regulates drought and salt tolerance in transgenic tobacco 苜蓿MsTIFY10a基因负调控转基因烟草的耐旱性和耐盐性

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-22 DOI: 10.1016/j.jplph.2025.154684

Qi Chen, Yan Zhang, Ying Tian, Jing Xu, Qing-Wen Fu, Zhen-Yi Li, Feng-Ling Shi, Cui-Ping Gao, Zhi-Qiang Zhang

The JAZ protein family acts as a key negative regulator in the jasmonic acid signaling pathway, interacting with transcription factors and playing essential roles in plant growth, development, and abiotic stress responses. However, the specific function of JAZ transcription factors in mediating salt and drought stress tolerance in alfalfa (Medicago sativa) remains unclear. In this study, we cloned MsTIFY10a, a JAZ gene from alfalfa, and found that its expression was downregulated under salt and drought stresses. Heterologous expression of MsTIFY10a in tobacco significantly reduced tolerance to both drought and salt stresses in seedlings and mature plants. Physiological analysis revealed that MsTIFY10a overexpression suppressed the antioxidant system, including superoxide dismutase (SOD) and peroxidase (POD), compromised photosynthetic capacity, and exacerbated membrane damage, collectively leading to reduced stress tolerance. Moreover, under drought or salt treatment, MsTIFY10a overexpression downregulated the expression of several reactive oxygen species (ROS)-related and stress-responsive genes. In summary, MsTIFY10a may functions as a negative regulator in abiotic stress responses, providing a basis for further investigation into its mechanistic roles in alfalfa.

JAZ蛋白家族是茉莉酸信号通路的关键负调控蛋白，与转录因子相互作用，在植物生长发育和非生物胁迫响应中发挥重要作用。然而，JAZ转录因子在苜蓿耐盐和干旱胁迫中的具体作用尚不清楚。本研究克隆了苜蓿JAZ基因MsTIFY10a，发现该基因在盐胁迫和干旱胁迫下表达下调。MsTIFY10a在烟草中的异源表达显著降低了烟草幼苗和成熟植株对干旱和盐胁迫的耐受性。生理分析表明，MsTIFY10a过表达抑制抗氧化系统，包括超氧化物歧化酶（SOD）和过氧化物酶（POD），降低光合能力，加剧膜损伤，共同导致胁迫耐受性降低。此外，在干旱或盐处理下，MsTIFY10a过表达下调了几种活性氧（ROS）相关基因和应激反应基因的表达。综上所述，MsTIFY10a可能在非生物胁迫响应中发挥负调控作用，为进一步研究其在苜蓿中的机制作用提供了基础。

{"title":"MsTIFY10a gene from alfalfa negatively regulates drought and salt tolerance in transgenic tobacco","authors":"Qi Chen, Yan Zhang, Ying Tian, Jing Xu, Qing-Wen Fu, Zhen-Yi Li, Feng-Ling Shi, Cui-Ping Gao, Zhi-Qiang Zhang","doi":"10.1016/j.jplph.2025.154684","DOIUrl":"10.1016/j.jplph.2025.154684","url":null,"abstract":"<div><div>The JAZ protein family acts as a key negative regulator in the jasmonic acid signaling pathway, interacting with transcription factors and playing essential roles in plant growth, development, and abiotic stress responses. However, the specific function of JAZ transcription factors in mediating salt and drought stress tolerance in alfalfa (<em>Medicago sativa</em>) remains unclear. In this study, we cloned <em>MsTIFY10a</em>, a <em>JAZ</em> gene from alfalfa, and found that its expression was downregulated under salt and drought stresses. Heterologous expression of <em>MsTIFY10a</em> in tobacco significantly reduced tolerance to both drought and salt stresses in seedlings and mature plants. Physiological analysis revealed that <em>MsTIFY10a</em> overexpression suppressed the antioxidant system, including superoxide dismutase (SOD) and peroxidase (POD), compromised photosynthetic capacity, and exacerbated membrane damage, collectively leading to reduced stress tolerance. Moreover, under drought or salt treatment, <em>MsTIFY10a</em> overexpression downregulated the expression of several reactive oxygen species (ROS)-related and stress-responsive genes. In summary, <em>MsTIFY10a</em> may functions as a negative regulator in abiotic stress responses, providing a basis for further investigation into its mechanistic roles in alfalfa.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"317 ","pages":"Article 154684"},"PeriodicalIF":4.1,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145839094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increasing CO2 concentration promoted the biomass accumulation but decreased the mineral nutrition and forage quality of Leymus chinensis CO2浓度的增加促进了羊草生物量的积累，但降低了羊草的矿质营养和饲料品质。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-19 DOI: 10.1016/j.jplph.2025.154680

Jie Yu , Cheng Ji , Yongwei Sun , Zhi Qi

The global atmospheric CO₂ concentration is predicted to increase from the current approximate 450 to 700 ppm by end of this century. To evaluate its potential impact on sustainability of grassland, Leymus chinensis, the dominant wild forage species in the eastern Eurasian Steppe, was cultivated in two growth chambers for a month with ambient 450 ppm (aCO₂) as the control and elevated 700 ppm CO₂ (eCO₂) as simulation of the future respectively. The eCO₂ increased the aboveground biomass, net photosynthesis rate and contents of carbohydrates, as well as the Mn contents in the leaves. The eCO₂ decreased the stomatal conductance, transpiration rate and the contents of mineral elements S, P, K, Fe, Zn in the xylem sap and leaves, as well as expression of ion transporter-encoding transcripts. As response to the eCO₂, the DNA, RNA and protein metabolism related transcripts were over-represented in the down-regulation transcriptome, accompanied with reduction in the contents of amino acids. The eCO₂ significantly suppressed expression of lipid metabolism-encoding transcripts and contents of phospholipids, as well as expression of vesicle-traffic encoding transcripts. The eCO₂ decreased contents of bioactive compounds flavonoids, terpenoids including gibberellins and steroids. These data imply that the predicted increasing atmospheric CO₂ concentration in the near future would have negative impacts on the forage quality of the grass.

预计到本世纪末，全球大气中的二氧化碳浓度将从目前的大约450 ppm增加到700 ppm。为了评估其对草地可持续性的潜在影响，以欧亚东部草原的优势野生牧草羊草（Leymus chinensis）为研究对象，在两个生长室内分别以450 ppm （aCO2）环境为对照和700 ppm （eCO2）升高为模拟未来，培养一个月。eCO2增加了地上生物量、净光合速率、碳水化合物含量和叶片中Mn含量。eCO2降低了木质部液和叶片中气孔导度、蒸腾速率和矿质元素S、P、K、Fe、Zn的含量，降低了离子转运蛋白编码转录物的表达。作为对eCO2的响应，DNA、RNA和蛋白质代谢相关转录本在下调转录组中被过度表达，同时氨基酸含量减少。eCO2显著抑制脂质代谢编码转录物的表达和磷脂含量，以及囊泡运输编码转录物的表达。eCO2降低了黄酮类化合物、萜类化合物（包括赤霉素和类固醇）的含量。这些数据表明，在不久的将来，预测的大气CO2浓度增加将对牧草的饲料质量产生负面影响。

{"title":"Increasing CO2 concentration promoted the biomass accumulation but decreased the mineral nutrition and forage quality of Leymus chinensis","authors":"Jie Yu , Cheng Ji , Yongwei Sun , Zhi Qi","doi":"10.1016/j.jplph.2025.154680","DOIUrl":"10.1016/j.jplph.2025.154680","url":null,"abstract":"<div><div>The global atmospheric CO<sub>2</sub> concentration is predicted to increase from the current approximate 450 to 700 ppm by end of this century. To evaluate its potential impact on sustainability of grassland, <em>Leymus chinensis</em>, the dominant wild forage species in the eastern Eurasian Steppe, was cultivated in two growth chambers for a month with ambient 450 ppm (aCO<sub>2</sub>) as the control and elevated 700 ppm CO<sub>2</sub> (eCO<sub>2</sub>) as simulation of the future respectively. The eCO<sub>2</sub> increased the aboveground biomass, net photosynthesis rate and contents of carbohydrates, as well as the Mn contents in the leaves. The eCO<sub>2</sub> decreased the stomatal conductance, transpiration rate and the contents of mineral elements S, P, K, Fe, Zn in the xylem sap and leaves, as well as expression of ion transporter-encoding transcripts. As response to the eCO<sub>2</sub>, the DNA, RNA and protein metabolism related transcripts were over-represented in the down-regulation transcriptome, accompanied with reduction in the contents of amino acids. The eCO<sub>2</sub> significantly suppressed expression of lipid metabolism-encoding transcripts and contents of phospholipids, as well as expression of vesicle-traffic encoding transcripts. The eCO<sub>2</sub> decreased contents of bioactive compounds flavonoids, terpenoids including gibberellins and steroids. These data imply that the predicted increasing atmospheric CO<sub>2</sub> concentration in the near future would have negative impacts on the forage quality of the grass.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"317 ","pages":"Article 154680"},"PeriodicalIF":4.1,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145834135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The adaptation strategy of Astragalus mongholicus shoots to the root Fe2+ deficiency and its strong stimulating effect on glucoliquiritin apioside accumulation 黄芪茎部对根系Fe2+缺乏的适应策略及其对糖醛酸苷苷积累的强烈刺激作用

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-19 DOI: 10.1016/j.jplph.2025.154679

Yanbing Dong , Yang Nan , Zhi Qi

Iron is an essential micronutrient. However, nearly 40 % of arable land worldwide suffers from iron deficiency. In this study, the adaptation of shoots to root Fe²⁺ deficiency was investigated using hydroponically grown Astragalus mongholicus, a widely cultivated medicinal plant. The root Fe²⁺ deficiency significantly inhibited the plants' growth and the contents of Fe, Mo, as well as 10 metabolites, including -isorhamnetin-3-O-glucoside, and nepitrin, which have reported anti-inflammatory activities. The root Fe²⁺ deficiency promoted the contents of Ca, Mg, K, Zn, Mn, and 12 metabolites, among which the glucoliquiritin apioside, an anti-infection flavonoid, strikingly increased by 2480.6 times. Transcriptome analysis revealed that Fe²⁺ deficiency could impair cellular energy metabolism by inhibiting the expression of ATPase and other essential enzymes for the tricarboxylic acid cycle. The plants adapted to the stress by enhancing the expression of transcripts encoding V-type H⁺-ATPases, Ca²⁺ and Mg²⁺ transporting ATPases, Fe²⁺ storage protein ferritins, as well as receptor like kinase and phytohormone-related transcription factors. In the transcriptome, a transcript encoding a functional Fe²⁺ passive transporter was identified by complementing Fe²⁺/Zn²⁺ uptake defective yeast mutants. The adaptation strategy of Astragalus mongholicus to the Fe²⁺ deficiency and the potential for increasing glucoliquiritin apioside contents in the shoots by occasionally applying Fe²⁺ chelators to the cultivating soils were discussed.

铁是一种必需的微量营养素。然而，全世界近40%的可耕地缺铁。以水培栽培的蒙古黄芪（Astragalus mongholicus）为研究对象，研究了其茎部对根系Fe2+缺乏的适应性。根系Fe2+缺乏显著抑制植株生长和铁、钼含量，以及-异鼠李素-3- o -葡萄糖苷、nepitrin等10种代谢产物的抗炎活性。根系缺铁促进了钙、镁、钾、锌、锰含量和12种代谢物的含量，其中抗感染类黄酮糖醛酸苷含量显著增加了2480.6倍。转录组分析显示，缺铁可能通过抑制atp酶和其他三羧酸循环必需酶的表达而损害细胞能量代谢。植物通过增强编码v型H+- atp酶、Ca2+和Mg2+转运atp酶、Fe2+储存蛋白铁蛋白以及受体激酶和植物激素相关转录因子的表达来适应逆境。在转录组中，通过补充Fe2+/Zn2+摄取缺陷酵母突变体，鉴定了一个编码功能性Fe2+被动转运蛋白的转录本。探讨了蒙古黄芪对Fe2+缺乏的适应策略，以及间或施用Fe2+螯合剂提高栽培土壤中糖醛酸苷含量的潜力。

{"title":"The adaptation strategy of Astragalus mongholicus shoots to the root Fe2+ deficiency and its strong stimulating effect on glucoliquiritin apioside accumulation","authors":"Yanbing Dong , Yang Nan , Zhi Qi","doi":"10.1016/j.jplph.2025.154679","DOIUrl":"10.1016/j.jplph.2025.154679","url":null,"abstract":"<div><div>Iron is an essential micronutrient. However, nearly 40 % of arable land worldwide suffers from iron deficiency. In this study, the adaptation of shoots to root Fe<sup>2+</sup> deficiency was investigated using hydroponically grown <em>Astragalus mongholicus</em>, a widely cultivated medicinal plant. The root Fe<sup>2+</sup> deficiency significantly inhibited the plants' growth and the contents of Fe, Mo, as well as 10 metabolites, including -isorhamnetin-3-O-glucoside, and nepitrin, which have reported anti-inflammatory activities. The root Fe<sup>2+</sup> deficiency promoted the contents of Ca, Mg, K, Zn, Mn, and 12 metabolites, among which the glucoliquiritin apioside, an anti-infection flavonoid, strikingly increased by 2480.6 times. Transcriptome analysis revealed that Fe<sup>2+</sup> deficiency could impair cellular energy metabolism by inhibiting the expression of ATPase and other essential enzymes for the tricarboxylic acid cycle. The plants adapted to the stress by enhancing the expression of transcripts encoding V-type H<sup>+</sup>-ATPases, Ca<sup>2+</sup> and Mg<sup>2+</sup> transporting ATPases, Fe<sup>2+</sup> storage protein ferritins, as well as receptor like kinase and phytohormone-related transcription factors. In the transcriptome, a transcript encoding a functional Fe<sup>2+</sup> passive transporter was identified by complementing Fe<sup>2+</sup>/Zn<sup>2+</sup> uptake defective yeast mutants. The adaptation strategy of <em>Astragalus mongholicus</em> to the Fe<sup>2+</sup> deficiency and the potential for increasing glucoliquiritin apioside contents in the shoots by occasionally applying Fe<sup>2+</sup> chelators to the cultivating soils were discussed.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"317 ","pages":"Article 154679"},"PeriodicalIF":4.1,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145966310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light signal transduction networks regulating phenylpropanoid, terpenoid and alkaloid biosynthesis in horticultural plants 园艺植物中调节苯丙类、萜类和生物碱生物合成的光信号转导网络

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-18 DOI: 10.1016/j.jplph.2025.154681

Yadi Chen, Lanxi Shi, Qingtao Xu, Chi Zhang, Li Wang, Weixing Li

Plant secondary metabolites (PSMs), crucial for horticultural crop quality and value, are synthesized in an organ-specific manner and are highly regulated by light. Acting beyond a mere energy source for photosynthesis, light signals are detected by specialized photoreceptors (e.g., phytochromes, cryptochromes, UV RESISTANCE LOCUS 8), triggering signaling cascades that converge on central regulators including the COP1-SPA complex and the transcription factor HY5. These regulators interact with a broad network of transcription factors, such as MYBs, bHLHs, BBXs, and PIFs, as well as epigenetic modifications, to precisely direct the transcriptional programs governing phenylpropanoid, terpenoid, and alkaloid metabolism. This review synthesizes these molecular mechanisms and discusses their implications for designing precise lighting strategies to enhance the quality and value of horticultural products in controlled-environment agriculture, thereby providing a theoretical foundation for light-quality regulation.

植物次生代谢物（psm）是一种对园艺作物品质和价值至关重要的物质，其合成具有器官特异性，并受光照高度调控。光信号不仅仅是光合作用的能量来源，还被专门的光感受器（如光敏色素、隐色素、抗紫外线位点8）检测到，触发信号级联，汇聚到包括COP1-SPA复合物和转录因子HY5在内的中心调节因子上。这些调节因子与广泛的转录因子网络相互作用，如myb、bHLHs、bbx和pif，以及表观遗传修饰，以精确指导控制苯丙素、萜类和生物碱代谢的转录程序。本文综述了这些分子机制，并讨论了它们对设计精确照明策略以提高受控环境农业园艺产品的质量和价值的意义，从而为光质量调控提供理论基础。

引用次数: 0

TaGSr-4D orchestrates lateral root development and tolerance to low nitrogen stress in Arabidopsis TaGSr-4D调控拟南芥侧根发育和对低氮胁迫的耐受。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2025-12-18 DOI: 10.1016/j.jplph.2025.154682

Huiqiang Li , Duheng Zhang , Xi Zhang , Furong Nai , Lulu Wang , Yihao Wei , Xiaochun Wang

Lateral roots are significant for capturing nutrients and water from the soil due to their capacity to expand the uptake area of the root system. Comprehending the molecular mechanisms that regulate lateral root development would be beneficial for optimizing the root system architecture (RSA) and improving crop yield. The enzyme GS (Glutamine synthetase) is a key enzyme that assimilates ammonium into glutamine. Previous study showed that TaGSr (Triticum aestivum L. ROOT GLUTAMINE SYNTHETASE) was mainly expressed in the root. However, little is known about the function of TaGSr in root system development in wheat. In this study, we showed that TaGSr-4D was expressed at all eight developmental stages of lateral root primordia and the heterologous expression of TaGSr-4D gene from wheat promoted the lateral root development in Arabidopsis. Overexpression of TaGSr-4D increased glutamine content and auxin content in root. Moreover, qRT-PCR analysis demonstrated that the expression of IAA14, LBD18, ARF6, ARF8, YUC3, YUC5, YUC6, and YUC9 were up-regulated in TaGSr-4D-OE Arabidopsis plants compared with wild-type. The absence of lateral roots in the arf7 arf19 mutant was not complemented by TaGSr-4D overexpression. These findings suggested that TaGSr-4D-regulated lateral root development is dependent on auxin signaling pathway. Furthermore, the shoot fresh weight of overexpression of TaGSr-4D OE-1 in Arabidopsis was greatly increased (39.29 %) compared with wild-type under low nitrogen conditions. This study may provides important clues for improving RSA and yield in wheat.

侧根对从土壤中捕获养分和水分具有重要意义，因为它们具有扩大根系吸收面积的能力。了解侧根发育的分子机制有助于优化根系结构，提高作物产量。谷氨酰胺合成酶（GS）是将氨同化为谷氨酰胺的关键酶。已有研究表明，Triticum aestivum L. ROOT GLUTAMINE SYNTHETASE （TaGSr）主要在根中表达。然而，对TaGSr在小麦根系发育中的作用知之甚少。在本研究中，我们发现TaGSr-4D基因在侧根原基的8个发育阶段均有表达，并且从小麦中外源表达TaGSr-4D基因促进了拟南芥侧根的发育。过表达TaGSr-4D增加了根中谷氨酰胺含量和生长素含量。qRT-PCR分析显示，与野生型相比，TaGSr-4D-OE拟南芥中IAA14、LBD18、ARF6、ARF8、YUC3、YUC5、YUC6和YUC9的表达上调。在arf7中，arf19突变体中侧根的缺失并没有被TaGSr-4D过表达所补充。这些发现表明，tagsr - 4d调控侧根发育依赖于生长素信号通路。低氮条件下，过表达TaGSr-4D OE-1的拟南芥茎鲜重较野生型显著增加（39.29%）。该研究可能为提高小麦的RSA和产量提供重要线索。

{"title":"TaGSr-4D orchestrates lateral root development and tolerance to low nitrogen stress in Arabidopsis","authors":"Huiqiang Li , Duheng Zhang , Xi Zhang , Furong Nai , Lulu Wang , Yihao Wei , Xiaochun Wang","doi":"10.1016/j.jplph.2025.154682","DOIUrl":"10.1016/j.jplph.2025.154682","url":null,"abstract":"<div><div>Lateral roots are significant for capturing nutrients and water from the soil due to their capacity to expand the uptake area of the root system. Comprehending the molecular mechanisms that regulate lateral root development would be beneficial for optimizing the root system architecture (RSA) and improving crop yield. The enzyme GS (Glutamine synthetase) is a key enzyme that assimilates ammonium into glutamine. Previous study showed that <em>TaGSr</em> (<em>Triticum aestivum</em> L. <em>ROOT GLUTAMINE SYNTHETASE</em>) was mainly expressed in the root. However, little is known about the function of <em>TaGSr</em> in root system development in wheat. In this study, we showed that <em>TaGSr-4D</em> was expressed at all eight developmental stages of lateral root primordia and the heterologous expression of <em>TaGSr-4D</em> gene from wheat promoted the lateral root development in Arabidopsis. Overexpression of <em>TaGSr-4D</em> increased glutamine content and auxin content in root. Moreover, qRT-PCR analysis demonstrated that the expression of <em>IAA14</em>, <em>LBD18</em>, <em>ARF6</em>, <em>ARF8</em>, <em>YUC3</em>, <em>YUC5</em>, <em>YUC6</em>, and <em>YUC9</em> were up-regulated in <em>TaGSr-4D-</em>OE Arabidopsis plants compared with wild-type. The absence of lateral roots in the <em>arf7 arf19</em> mutant was not complemented by <em>TaGSr-4D</em> overexpression. These findings suggested that <em>TaGSr-4D</em>-regulated lateral root development is dependent on auxin signaling pathway. Furthermore, the shoot fresh weight of overexpression of <em>TaGSr-4D</em> OE-1 in Arabidopsis was greatly increased (39.29 %) compared with wild-type under low nitrogen conditions. This study may provides important clues for improving RSA and yield in wheat.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"317 ","pages":"Article 154682"},"PeriodicalIF":4.1,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0