Journal of plant physiology最新文献_第4页

The JmjC domain-containing histone demethylase ZmJMJ703 orchestrates salt stress adaptation in maize 含有JmjC结构域的组蛋白去甲基酶ZmJMJ703调控玉米的盐胁迫适应

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-02-01 Epub Date: 2025-12-15 DOI: 10.1016/j.jplph.2025.154677

Shu Wang , Lijun Jiang , Tingting Zhai , Ke Qu , Xingyu Liu , Zhaomeng Di , Yingshan Chen , Xiaoduo Lu , Xiang Li , Jiedao Zhang , Shuxin Zhang , Wei Yang

The JmjC domain-containing protein family (JMJs) represents a family of key demethylases critical for epigenetic regulation and orchestrating plant growth and developmental processes. Despite their established roles, functional investigations into JMJ proteins under abiotic stress conditions remain limited in maize. In this study, we identified and functionally characterized ZmJMJ703, a JmjC domain-containing gene exhibiting salt stress-responsive expression patterns in maize. Physiological and phenotypic analysis revealed that ZmJMJ703 mutation significantly impairs salt stress tolerance in maize seedlings. Transcriptomic profiling uncovered differential expression patterns between zmjmj703 mutants and wild-type plants, with affected genes predominantly associated with intracellular protein trafficking, amino acid metabolism, and small molecule reprogramming. Parallel proteomic analysis through mass spectrometry further demonstrated that differential protein accumulation in mutants primarily enriched pathways related to secondary metabolite biosynthesis. These integrated omics analyses collectively suggest that ZmJMJ703 may modulate metabolic pathways critical for abiotic stress responses. Functional validation was reinforced by phenotypic evaluation of Arabidopsis lines heterologous overexpressing ZmJMJ703, which exhibited enhanced salt stress tolerance compared to control plants. Collectively, these findings significantly advance our mechanistic understanding of JMJ proteins' contributions to plant abiotic stress resilience, particularly in the context of salt stress adaptation.

JmjC结构域蛋白家族（JMJs）代表了一个对表观遗传调控和协调植物生长发育过程至关重要的关键去甲基化酶家族。尽管JMJ蛋白具有既定的作用，但对其在非生物胁迫条件下在玉米中的功能研究仍然有限。在这项研究中，我们鉴定并功能表征了ZmJMJ703，这是一个含JmjC结构域的基因，在玉米中表现出盐胁迫响应的表达模式。生理和表型分析表明，ZmJMJ703突变显著降低了玉米幼苗的耐盐性。转录组学分析揭示了zmjmj703突变体与野生型植物之间的差异表达模式，受影响的基因主要与细胞内蛋白质运输、氨基酸代谢和小分子重编程相关。通过质谱分析的平行蛋白质组学分析进一步表明，突变体中蛋白质的差异积累主要富集了与次生代谢物生物合成相关的途径。这些综合组学分析共同表明，ZmJMJ703可能调节对非生物应激反应至关重要的代谢途径。通过对异源过表达ZmJMJ703的拟南芥品系进行表型评价，证实其耐盐性强于对照植株。总的来说，这些发现显著地促进了我们对JMJ蛋白对植物非生物胁迫恢复能力的贡献的机制理解，特别是在盐胁迫适应的背景下。

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引用次数: 0

Spatiotemporal trajectory of senescence in mesocarp cell clusters of Hylocereus undatus based on single-cell and spatial transcriptomics 基于单细胞和空间转录组学的黄颡鱼中果皮细胞群衰老的时空轨迹

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-02-01 Epub Date: 2025-12-12 DOI: 10.1016/j.jplph.2025.154672

Yajing Tian , Xiang Ji , Mingyue Lv , Lili Lu , Tengfei Yu , Jingya Wang , Jingyu Xu , Guanzhi Wang , Fuqiang Li , Yiyang Song , Yang Li , Xinyue Pang , Xin Li

Fruit senescence is a complex physiological process. Single-cell RNA sequencing (scRNA-seq) analysis revealed the differentiation trajectories of 13 cell clusters during the senescence of Hylocereus undatus (H. undatus). The mesocarp of the fruit contained four cell clusters, but their precise localization and functional division remained unclear. This work documented mesocarp phenotypic alterations and elucidated the time courses of mesocarp flavonoid biosynthesis and superoxide anion generation. Additionally, overall ROS changes were observed using fluorescence microscopy. By combining the single-cell atlas with spatial transcriptomics data at resolutions of 0.2 and 0.8, and applying four computational algorithms (SingleR, SciBet, CARD, and RCTD), we accurately mapped the spatial distribution of the four cell populations in the two layers of the mesocarp from outer to inner regions. Furthermore, we identified highly correlated cells with cell-specific functions, which allowed us to perform a detailed analysis of the differentiation trajectories of these four cell clusters. We proposed a hypothesis that these four clusters in the mesocarp participate in the senescence process. Finally, using SCODE, we uncovered the gene regulatory networks of the pericarp's highly correlated cell clusters during fruit senescence. Through single-cell technology, the functional division of the four cell clusters in the mesocarp—responsible for stress responses, signal transduction, material preparation, and cell differentiation trajectories—has been revealed. These findings provide insights from a single-cell dimension and a spatiotemporal perspective, enhancing the understanding of the dynamic process of plant senescence.

果实衰老是一个复杂的生理过程。单细胞RNA测序（scRNA-seq）分析揭示了13个细胞簇在水仙花（H. undatus）衰老过程中的分化轨迹。果实的中果皮包含四个细胞簇，但它们的精确定位和功能分裂尚不清楚。本文记录了中果皮的表型变化，阐明了中果皮类黄酮生物合成和超氧阴离子生成的时间过程。此外，利用荧光显微镜观察总体ROS变化。通过将单细胞图谱与分辨率为0.2和0.8的空间转录组学数据相结合，并应用4种计算算法（SingleR、SciBet、CARD和RCTD），我们精确地绘制了中果皮两层中4种细胞群体从外到内的空间分布。此外，我们确定了与细胞特异性功能高度相关的细胞，这使我们能够对这四个细胞簇的分化轨迹进行详细分析。我们提出一个假设，这四个簇在中果皮参与衰老过程。最后，我们利用SCODE揭示了果皮高度相关的细胞团在果实衰老过程中的基因调控网络。通过单细胞技术，研究人员揭示了中果皮中负责应激反应、信号转导、物质制备和细胞分化轨迹的四种细胞簇的功能分裂。这些发现提供了从单细胞维度和时空角度的见解，增强了对植物衰老动态过程的理解。

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引用次数: 0

Screening frost-tolerant kale (Brassica oleracea L. var. acephala) genotypes through cold-responsive metabolic changes in open field conditions 通过大田条件下冷响应代谢变化筛选耐冻甘蓝基因型

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-02-01 Epub Date: 2026-01-09 DOI: 10.1016/j.jplph.2026.154695

Lovro Sinkovič , Barbara Pipan , Mohamed Neji , Hajer Ben Ammar , Vladimir Meglič , Robert Veberič , Ana Slatnar , Jerneja Jakopič

Kale (Brassica oleracea L. var. acephala) is a cold-tolerant leafy vegetable whose metabolic plasticity under frost stress remains underexplored. In this study, leaf tissues from 26 kale accessions grown under open-field conditions were metabolically profiled, focusing on soluble sugars, glucosinolates, and photosynthetic pigments before and after exposure to short-term frost. Frost stress induced significant quantitative and compositional shifts in sugar profiles, notably an accumulation of sucrose, suggesting its role as a key osmoprotectant. Among the twelve glucosinolates identified, indolic compounds dominated the unfrosted profile (67.4 %) but declined post-frost (51.6 %), coinciding with a marked increase in aliphatic glucosinolates (from 20.7 % to 38.6 %). Chlorophyll and carotenoid contents declined in most accessions following frost exposure. Notably, the metabolic profile of Accession_4 indicates a potentially resilient phenotype, characterised by limited pigment degradation and a shift toward aliphatic glucosinolates. This may reflect a stress-adaptation strategy and could be explored as a candidate for breeding or metabolotype selection approaches.

羽衣甘蓝（Brassica oleracea L. var. acephala）是一种耐寒的叶菜，其在霜冻胁迫下的代谢可塑性尚不充分。在本研究中，对26份在露天条件下生长的羽衣甘蓝叶片组织进行了代谢分析，重点研究了短期霜冻前后可溶性糖、硫代葡萄糖苷和光合色素的代谢变化。霜冻胁迫诱导糖谱发生了显著的数量和成分变化，尤其是蔗糖的积累，这表明它是一种关键的渗透保护剂。在鉴定的12种硫代葡萄糖苷中，吲哚类化合物在未霜冻的情况下占主导地位（67.4%），但霜冻后含量下降（51.6%），与脂肪族硫代葡萄糖苷显著增加（从20.7%增加到38.6%）相一致。多数品种的叶绿素和类胡萝卜素含量在霜冻后下降。值得注意的是，Accession_4的代谢谱显示出一种潜在的弹性表型，其特征是有限的色素降解和向脂肪族硫代葡萄糖苷的转变。这可能反映了一种压力适应策略，可以作为育种或代谢型选择方法的候选物。

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引用次数: 0

Multi-omics profiling identifies potential biological nitrification inhibitor 1,9-decanediol biosynthesis and secretion mechanisms in diverse rice varieties 多组学分析鉴定了不同水稻品种潜在的生物硝化抑制剂1,9-十二烷二醇的生物合成和分泌机制。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-02-01 Epub Date: 2026-01-02 DOI: 10.1016/j.jplph.2026.154688

Dong-Wei Di , Chi-Shang Luan , Ming-Kun Ma , Ting Yang , Herbert J. Kronzucker , Ju Min , Yufang Lu , Xiaofei Liu , Weiming Shi

1,9-Decanediol (1,9-D) was identified as the first fatty alcohol with biological nitrification inhibition (BNI) activity in rice root exudates. In a previous study, transcriptomic and untargeted metabolomic analyses contrasted two rice varieties, suggesting metabolic pathways and associated gene families involved in 1,9-D biosynthesis and secretion. However, due to the small number of varieties and technical constraints, the pool of candidate genes remained extensive, impeding precise identification and functional validation. Here, we employ ten rice varieties with diverging root-secretion properties for integrated transcriptomic and targeted metabolomic profiling. Our results demonstrate that ammonium treatment significantly enhances 1,9-D secretion across varieties, indicating a potentially conserved regulatory mechanism in rice. Integrated analysis revealed that α-linolenic acid (LN) and linoleic acid (LA) may serve as biosynthetic precursors of 1,9-D and identified key candidate genes in LN/LA metabolism, including Os04g37430, Os04g47120, Os08g39840, Os08g39850, and Os08g08220. Furthermore, our data show that root secretion of 1,9-D is actively regulated by specific transporters, including the major facilitator superfamily (MFS) member Os11g04104, the ATP-binding cassette (ABC) transporter Os01g07870, and the multidrug and toxic compound extrusion (MATE) transporter Os10g20390. This study reveals a novel multi-gene regulatory network underlying 1,9-D synthesis and secretion, providing candidate targets for improving nitrogen-use efficiency in rice.

1,9-癸二醇（1,9- d）是水稻根系分泌物中第一个具有生物硝化抑制（BNI）活性的脂肪醇。在之前的研究中，转录组学和非靶向代谢组学分析对比了两个水稻品种，发现了参与1,9- d生物合成和分泌的代谢途径和相关基因家族。然而，由于品种较少和技术限制，候选基因池仍然广泛，阻碍了精确鉴定和功能验证。在这里，我们采用了10个具有不同根分泌特性的水稻品种进行综合转录组学和靶向代谢组学分析。我们的研究结果表明，铵处理显著提高了水稻品种间1,9- d的分泌，表明了一个潜在的保守调控机制。综合分析发现α-亚麻酸（LN）和亚油酸（LA）可能是1,9- d的生物合成前体，并鉴定出参与LN/LA代谢的关键候选基因Os04g37430、Os04g47120、Os08g39840、Os08g39850和Os08g08220。此外，我们的数据显示，1,9- d的根分泌受到特定转运体的积极调节，包括主要促进剂超家族（MFS）成员Os11g04104、atp结合盒（ABC）转运体Os01g07870和多药和有毒化合物挤出（MATE）转运体Os10g20390。该研究揭示了1,9- d合成和分泌的一个新的多基因调控网络，为提高水稻氮素利用效率提供了候选靶点。

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引用次数: 0

Molecular mechanisms and biotechnological advances in herbicide resistance: Insights into the development of herbicide-tolerant crops 抗除草剂的分子机制和生物技术进展：对耐除草剂作物发展的见解。

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-02-01 Epub Date: 2026-01-05 DOI: 10.1016/j.jplph.2026.154690

Sheng Zheng , Haiyan Tie , Shangli Chai, Mingxiao Wang, Sirui Wang, Yuan-Yuan Zeng, Guofan Wu, Teng-Guo Zhang

Herbicides play a pivotal role in modern agriculture by controlling weed populations and safeguarding crop yields. However, the long-term and extensive use of herbicides has accelerated the evolution of herbicide-resistant weeds, thereby diminishing their efficacy and posing a serious threat to global food security. Recent advances in molecular biology and plant biotechnology have greatly expanded our understanding of herbicide resistance mechanisms and enabled the development of crops with enhanced herbicide resistance. Herbicide resistance genes function primarily by encoding detoxifying enzymes, modifying herbicide target sites, or activating specific metabolic pathways that mitigate herbicidal toxicity. Emerging genetic tools, including transgenic approaches and CRISPR/Cas-mediated genome editing, have further facilitated the precise introduction of resistance traits into major crops. It is noteworthy that this review offers novel insights into the latest CRISPR/Cas applications, including base editing and prime editing for developing novel, non-transgenic herbicide-resistant crops. Furthermore, it provides a systematic overview of advanced strategies for engineering multi-gene stacking traits to combat complex or evolving weed resistance. This review integrates recent progress in elucidating the molecular targets of herbicides and the underlying resistance mechanisms, and highlights the potential of modern biotechnological strategies for engineering herbicide-resistant crops to promote sustainable and environmentally responsible weed management.

除草剂在现代农业中起着控制杂草数量和保障作物产量的关键作用。然而，长期和广泛使用除草剂加速了抗除草剂杂草的进化，从而降低了其药效，对全球粮食安全构成严重威胁。分子生物学和植物生物技术的最新进展极大地扩展了我们对除草剂抗性机制的理解，并使开发具有增强抗除草剂能力的作物成为可能。除草剂抗性基因主要通过编码解毒酶、修饰除草剂靶点或激活特定的代谢途径来减轻除草剂毒性。新兴的遗传工具，包括转基因方法和CRISPR/ cas介导的基因组编辑，进一步促进了将抗性性状精确引入主要作物。值得注意的是，本综述为CRISPR/Cas的最新应用提供了新的见解，包括碱基编辑和引体编辑在开发新型非转基因抗除草剂作物中的应用。此外，它提供了一个系统的概述了先进的策略，工程多基因堆叠性状，以对抗复杂的或不断发展的杂草抗性。本文综述了除草剂分子靶点和潜在抗性机制的最新研究进展，并强调了现代生物技术策略在抗除草剂作物工程方面的潜力，以促进可持续和对环境负责的杂草管理。

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引用次数: 0

Eggplant SmMYB6.2 positively regulates anthocyanin biosynthesis by activating SmANS gene expression 茄子SmMYB6.2通过激活SmANS基因表达正向调节花青素生物合成

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-02-01 Epub Date: 2025-12-13 DOI: 10.1016/j.jplph.2025.154676

Jiangnan Hao, Ziyi Hua, Jinwei Zhang, Sufen Liu, Dalu Li, Shaohang Li, Yang Liu, Huoying Chen

Anthocyanins significantly influence both the visual quality and nutritional value of eggplants. Exogenous application of jasmonic acid enhanced anthocyanin biosynthesis in eggplant peel under low-light conditions and induced the expression of several MYB genes. In this paper, these MYB proteins were investigated by yeast one-hybrid experiments, and it was found that SmMYB6.2 could directly bind to the promoter sequence of the anthocyanin synthesis structural gene SmANS. SmMYB6.2 was a nuclear-localized protein whose expression could be induced by various stimuli, including UV-B radiation, blue light, ABA treatment, PEG stress, and low-temperature exposure at 4 °C. Next, overexpression of SmMYB6.2 in Arabidopsis promoted anthocyanin accumulation and enhanced the gene expression of AtANS. Further, Dual-LUC assays demonstrated that SmMYB6.2 enhanced its transcriptional activation of the SmANS promoter through protein-protein interactions with the bHLH proteins SmTT8, SmbHLH79, and SmGLABRA3. These findings deepen our understanding of the regulatory mechanisms underlying anthocyanin biosynthesis in eggplant peel and provide candidate genes for breeding anthocyanin-enriched eggplant varieties.

花青素对茄子的视觉品质和营养价值均有显著影响。外源施用茉莉酸促进了弱光条件下茄子皮花青素的合成，诱导了多个MYB基因的表达。本文通过酵母单杂交实验对这些MYB蛋白进行了研究，发现SmMYB6.2可以直接结合花青素合成结构基因SmANS的启动子序列。SmMYB6.2是一种核定位蛋白，可以在各种刺激下诱导表达，包括UV-B辐射、蓝光、ABA处理、PEG胁迫和4°C低温暴露。接下来，SmMYB6.2在拟南芥中的过表达促进了花青素的积累，增强了AtANS基因的表达。此外，Dual-LUC分析表明，SmMYB6.2通过与bHLH蛋白SmTT8、SmbHLH79和SmGLABRA3的蛋白相互作用增强了其对SmANS启动子的转录激活。这些发现加深了我们对茄子皮花青素生物合成调控机制的理解，并为培育富含花青素的茄子品种提供了候选基因。

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引用次数: 0

MsTIFY10a gene from alfalfa negatively regulates drought and salt tolerance in transgenic tobacco 苜蓿MsTIFY10a基因负调控转基因烟草的耐旱性和耐盐性

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-02-01 Epub Date: 2025-12-22 DOI: 10.1016/j.jplph.2025.154684

Qi Chen, Yan Zhang, Ying Tian, Jing Xu, Qing-Wen Fu, Zhen-Yi Li, Feng-Ling Shi, Cui-Ping Gao, Zhi-Qiang Zhang

The JAZ protein family acts as a key negative regulator in the jasmonic acid signaling pathway, interacting with transcription factors and playing essential roles in plant growth, development, and abiotic stress responses. However, the specific function of JAZ transcription factors in mediating salt and drought stress tolerance in alfalfa (Medicago sativa) remains unclear. In this study, we cloned MsTIFY10a, a JAZ gene from alfalfa, and found that its expression was downregulated under salt and drought stresses. Heterologous expression of MsTIFY10a in tobacco significantly reduced tolerance to both drought and salt stresses in seedlings and mature plants. Physiological analysis revealed that MsTIFY10a overexpression suppressed the antioxidant system, including superoxide dismutase (SOD) and peroxidase (POD), compromised photosynthetic capacity, and exacerbated membrane damage, collectively leading to reduced stress tolerance. Moreover, under drought or salt treatment, MsTIFY10a overexpression downregulated the expression of several reactive oxygen species (ROS)-related and stress-responsive genes. In summary, MsTIFY10a may functions as a negative regulator in abiotic stress responses, providing a basis for further investigation into its mechanistic roles in alfalfa.

JAZ蛋白家族是茉莉酸信号通路的关键负调控蛋白，与转录因子相互作用，在植物生长发育和非生物胁迫响应中发挥重要作用。然而，JAZ转录因子在苜蓿耐盐和干旱胁迫中的具体作用尚不清楚。本研究克隆了苜蓿JAZ基因MsTIFY10a，发现该基因在盐胁迫和干旱胁迫下表达下调。MsTIFY10a在烟草中的异源表达显著降低了烟草幼苗和成熟植株对干旱和盐胁迫的耐受性。生理分析表明，MsTIFY10a过表达抑制抗氧化系统，包括超氧化物歧化酶（SOD）和过氧化物酶（POD），降低光合能力，加剧膜损伤，共同导致胁迫耐受性降低。此外，在干旱或盐处理下，MsTIFY10a过表达下调了几种活性氧（ROS）相关基因和应激反应基因的表达。综上所述，MsTIFY10a可能在非生物胁迫响应中发挥负调控作用，为进一步研究其在苜蓿中的机制作用提供了基础。

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引用次数: 0

Coordinated systemic regulation maintains plant nutrient homeostasis 协调的系统调节维持植物营养平衡

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-02-01 Epub Date: 2026-01-05 DOI: 10.1016/j.jplph.2026.154691

Qian-Qian Li, Yi-Qun Gao

Plant survival in fluctuating environments depends on the precise maintenance of nutrient homeostasis, which requires the integration of local nutrient availability with whole-plant demand. While the molecular components of local nutrient sensing and uptake are well-characterized, the systemic signaling networks that coordinate acquisition, allocation, and utilization across distant organs are less understood. This review synthesizes recent advances in understanding the systemic regulation of plant nutrient homeostasis. We highlight how plants coordinate photosynthetic activity in shoots with mineral nutrient absorption in roots to optimize growth. Furthermore, we examine the long-distance communication systems through which diverse mobile signals are translocated via the vasculature to synchronize root nutrient uptake with shoot photosynthetic status and developmental demands. Collectively, this synthesis illuminates the complex and finely tuned regulatory mechanisms that balance growth with environmental adaptation.

植物在波动环境中的生存依赖于营养稳态的精确维持，这需要将当地的营养供应与整个植物的需求结合起来。虽然局部营养感知和吸收的分子成分已被很好地表征，但协调远端器官获取、分配和利用的系统信号网络尚不清楚。本文综述了植物营养平衡系统调控的最新研究进展。我们强调了植物如何协调芽部的光合作用与根系的矿质养分吸收以优化生长。此外，我们还研究了远距离通信系统，通过该系统，各种移动信号通过脉管系统进行转运，以同步根营养吸收与茎部光合状态和发育需求。总的来说，这种综合阐明了平衡生长与环境适应的复杂而精细的调节机制。

引用次数: 0

The far-red light absorption and “Redundant chlorophyll” in plants: A waste of resource or an important booster of photosynthesis? 植物对远红光的吸收与“多余叶绿素”：资源浪费还是光合作用的重要助推器？

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-01 Epub Date: 2025-12-03 DOI: 10.1016/j.jplph.2025.154669

Alexei Solovchenko , Anatoly Gitelson

Mature non-stressed plants often contain a lot more chlorophyll than they need to efficiently capture light energy in the PAR range. In this situation, some pigment molecules apparently become physiologically redundant because they remain shaded and cannot participate efficiently in light harvesting. As a result of the build-up of chlorophyll, strong absorption of these pigments extends well beyond 700 nm, the conventional border of PAR, into far red (FR) region of the spectrum (to 750 nm and beyond) contributing significantly to the budget of the absorbed light energy. It is also well known that FR light, when supplemented to conventional PAR spectrum, harmonizes energy flow in the photosynthetic apparatus, reduces risk of photodamage boosting plant productivity. We argue that a possible functional role of the “redundant chlorophyll” accumulated in plants is ensuring the capture of FR photons. The latter is among important acclimations to fluctuating light fluxes as well as to permanently low-light environments ensuring efficient operation of complex plant canopies. We discuss the opportunity to harness the “FR boost” of productivity by leveraging inherent optical properties of green plants without sophisticated approaches such as engineering of long-wave chlorophylls into the plant photosynthetic apparatus.

成熟的非胁迫植物通常含有比它们在PAR范围内有效捕获光能所需的更多的叶绿素。在这种情况下，一些色素分子显然在生理上变得多余，因为它们保持阴影状态，不能有效地参与光收集。由于叶绿素的积累，这些色素的强吸收远远超出了PAR的传统边界700nm，进入光谱的远红（FR）区域（至750nm及以上），大大增加了吸收光能的预算。众所周知，当FR光补充到常规PAR光谱时，可以协调光合装置中的能量流动，降低光损伤的风险，提高植物的生产力。我们认为，在植物中积累的“多余叶绿素”的一个可能的功能作用是确保FR光子的捕获。后者是适应波动光通量和永久低光环境的重要适应之一，确保复杂植物冠层的有效运行。我们讨论了利用绿色植物固有的光学特性来利用“FR促进”生产力的机会，而无需复杂的方法，如将长波叶绿素工程引入植物光合装置。

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引用次数: 0

Deficiency of Arabidopsis SMO2 triggers an ANAC082-Dependent pathway to regulate root apical meristem 拟南芥SMO2缺失触发anac082依赖通路调控根尖分生组织

IF 4.1 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology

Pub Date : 2026-01-01 Epub Date: 2025-11-19 DOI: 10.1016/j.jplph.2025.154651

Aiming Xing , Xiaoyu Wang , Jing Gao , Cheng Zeng , Ning Li , Chunfei Wang , Xiaoqing Wang , Zhenguo Shen , Zhubing Hu

Root apical meristem (RAM) maintains sustained root development, which is intricately coordinated by a series of biological processes integrating internal and external cues. Here, we present evidence that SMALL ORGAN 2 (SMO2) is required for the maintenance of RAM. Deficiency of SMO2 resulted in disorganization of the stem cell niche (SCN) and the presence of dead cells in RAM, independent of DNA damage. Moreover, following chemically induced wounding, excision of root tips, or removal of whole roots, the regeneration capacity of smo2 mutants was dramatically lower than that of wild-type plants. Mechanistically, SMO2 is necessary for 18S rRNA methylation at position G1581, which may be associated with abnormal ribosome profiles in smo2 mutants. Further analysis showed that deficiency of SMO2 markedly reduced the translation efficiency of genes involved in polar auxin transport, thereby reducing auxin accumulation in the RAM. Surprisingly, an additional deletion of ANAC082, which encodes a known transcriptional mediator of ribosomal stress-induced developmental adaptations, effectively restored the disordered SCN and cell death phenotypes in smo2 mutants. Collectively, our findings demonstrate that SMO2-mediated translational regulation plays an important role in auxin accumulation in root tips. This process appears to be essential for sustaining RAM activity, as indicated by the structural disorganization of root tips in smo2 mutants. Notably, this phenotypic manifestation is mechanistically dependent on ANAC082.

根尖分生组织（Root apical merisystem， RAM）维持着根系的持续发育，这一过程是由一系列复杂的生物过程协调而成的。在这里，我们提出的证据表明，小器官2 （SMO2）是维持RAM所必需的。SMO2的缺乏导致RAM中干细胞生态位（SCN）的破坏和死细胞的存在，而这与DNA损伤无关。此外，在化学诱导损伤、根尖切除或全根切除后，smo2突变体的再生能力显著低于野生型植株。从机制上讲，SMO2是G1581位点18S rRNA甲基化的必要条件，这可能与SMO2突变体中异常的核糖体谱有关。进一步分析表明，缺乏SMO2显著降低了参与生长素极性转运的基因的翻译效率，从而减少了生长素在RAM中的积累。令人惊讶的是，ANAC082的额外缺失，编码核糖体应激诱导的发育适应的已知转录介质，有效地恢复了smo2突变体中紊乱的SCN和细胞死亡表型。总之，我们的研究结果表明，smo2介导的翻译调控在根尖生长素积累中起着重要作用。这一过程似乎是维持RAM活性所必需的，正如smo2突变体根尖结构紊乱所表明的那样。值得注意的是，这种表型表现在机制上依赖于ANAC082。

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引用次数: 0