Pub Date : 2022-08-03DOI: 10.1080/17429145.2022.2107722
M. Aslam, M. Waris, Ihsan Muhammad, Maqbool Ahmed, Z. Khan, Z. Jabeen, Mohammad Yakoob Zehri, M. Arsalan, S. Rehman, A. M. Alnasrawi, Jawaher Alkahtani, M. S. Elshikh, Muhammad Rizwan, Shoaib Raza, Jinsong Deng, Adnan Raza Altaf
ABSTRACT Lead (Pb) is a biologically non-essential element in the soil that brutally affects plants and other living organisms in soil; hence, its removal has become a worldwide concern. In this work, a multifunctional nanoscale zerovalent-iron assisted biochar (nFe°/BC) was used to minimize the Pb bioavailability in soil with aim of alleviating the Pb-induced toxicity in sunflower. Results revealed that nFe°/BC treatment had significantly improved plant growth (58%), chlorophyll contents (66%), intracellular permeability (60%), and ratio factor (93%), while decreasing the Pb uptake (78%) in plants. The Pb-immobilization and transformation mechanisms were proposed, suggesting that the presence of organic functional groups over the nFe°/BC surface might induce the complex formation with Pb by the ions exchange process in soil solution. The XPS analysis confirmed that surface-active components (Fe+, O2−, O*, C═O) were the key factor for high Pb-immobilization within soil matrix. In addition, 87% of stable Pb species, including PbCO3, PbO, Pb (OH)2, and Pb-O-Fe were found in the soil surface. Current findings have exposed the diverse functions of nFe°/BC on plant health and established a phenomenon that nFe°/BC application could improve the plant agronomic attributes by regulating the homeostasis of antioxidants and Pb uptake.
铅(Pb)是土壤中的一种生物非必需元素，对土壤中的植物和其他生物具有严重影响;因此，它的清除已成为全世界关注的问题。在本研究中，利用纳米多功能零价铁辅助生物炭(nFe°/BC)降低土壤中铅的生物有效性，以减轻铅对向日葵的毒性。结果表明，nFe°/BC处理显著提高了植物生长(58%)、叶绿素含量(66%)、细胞内通透性(60%)和比值因子(93%)，降低了植物对Pb的吸收(78%)。研究结果表明，nFe°/BC表面有机官能团的存在可能导致土壤溶液中离子交换过程中与Pb形成络合物。XPS分析证实，表面活性成分(Fe+， O2−，O*， C = O)是土壤基质内高铅固定的关键因素。此外，土壤表层有87%的Pb稳定种，包括PbCO3、PbO、Pb (OH)2和Pb- o - fe。目前的研究结果揭示了nFe°/BC对植物健康的多种作用，并确定了nFe°/BC可以通过调节抗氧化剂和铅吸收的动态平衡来改善植物农艺性状的现象。
{"title":"Lead-Immobilization, transformation, and induced toxicity alleviation in sunflower using nanoscale Fe°/BC: Experimental insights with Mechanistic validations","authors":"M. Aslam, M. Waris, Ihsan Muhammad, Maqbool Ahmed, Z. Khan, Z. Jabeen, Mohammad Yakoob Zehri, M. Arsalan, S. Rehman, A. M. Alnasrawi, Jawaher Alkahtani, M. S. Elshikh, Muhammad Rizwan, Shoaib Raza, Jinsong Deng, Adnan Raza Altaf","doi":"10.1080/17429145.2022.2107722","DOIUrl":"https://doi.org/10.1080/17429145.2022.2107722","url":null,"abstract":"ABSTRACT Lead (Pb) is a biologically non-essential element in the soil that brutally affects plants and other living organisms in soil; hence, its removal has become a worldwide concern. In this work, a multifunctional nanoscale zerovalent-iron assisted biochar (nFe°/BC) was used to minimize the Pb bioavailability in soil with aim of alleviating the Pb-induced toxicity in sunflower. Results revealed that nFe°/BC treatment had significantly improved plant growth (58%), chlorophyll contents (66%), intracellular permeability (60%), and ratio factor (93%), while decreasing the Pb uptake (78%) in plants. The Pb-immobilization and transformation mechanisms were proposed, suggesting that the presence of organic functional groups over the nFe°/BC surface might induce the complex formation with Pb by the ions exchange process in soil solution. The XPS analysis confirmed that surface-active components (Fe+, O2−, O*, C═O) were the key factor for high Pb-immobilization within soil matrix. In addition, 87% of stable Pb species, including PbCO3, PbO, Pb (OH)2, and Pb-O-Fe were found in the soil surface. Current findings have exposed the diverse functions of nFe°/BC on plant health and established a phenomenon that nFe°/BC application could improve the plant agronomic attributes by regulating the homeostasis of antioxidants and Pb uptake.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43031122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-03DOI: 10.1080/17429145.2022.2106385
Yuan Ye, S. Hongwei, Wang Yue, Xu Zisong, Han Shixin, He Guoqiang, Yin Kuide, Huihui Zhang
ABSTRACT We used chlorophyll fluorescence technology and biochemical methods to analyze the effects of wood vinegar (WV) on the photosynthetic mechanism and reactive oxygen species metabolism of tobacco (Nicotiana tabacum L.) leaves infected by Pseudomonas syringae pv. tabaci (Pst). The results showed that Pst infection reduced the chlorophyll content and the activities of PSII and PSI, which inhibited the normal photosynthesis of tobacco leaves. However, pretreatment with WV alleviated the degradation of chlorophyll. Treatment with WV alleviated the downregulation of core gene expression in PSII and PSI and improved the photosynthetic electron transfer in Pst-infected leaves. The levels of expression of PetE, ATPa and ATPc in the Pst-infected leaves were significantly upregulated when pretreated with WV. Pst infection increased the rate of production of superoxide anions and the contents of hydrogen peroxide. WV pretreatment could eliminate the oxidative damage of Pst-infected leaves by enhancing the activities of peroxidase (POD) and glutathione peroxidase (GPx) and upregulating the levels of expression of the POD2 and GPX2 genes. In conclusion, pretreatment with WV can alleviate the photosynthetic inhibition and oxidative damage of tobacco leaves caused by Pst infection.
{"title":"Wood vinegar alleviates photosynthetic inhibition and oxidative damage caused by Pseudomonas syringae pv. tabaci (Pst) infection in tobacco leaves","authors":"Yuan Ye, S. Hongwei, Wang Yue, Xu Zisong, Han Shixin, He Guoqiang, Yin Kuide, Huihui Zhang","doi":"10.1080/17429145.2022.2106385","DOIUrl":"https://doi.org/10.1080/17429145.2022.2106385","url":null,"abstract":"ABSTRACT We used chlorophyll fluorescence technology and biochemical methods to analyze the effects of wood vinegar (WV) on the photosynthetic mechanism and reactive oxygen species metabolism of tobacco (Nicotiana tabacum L.) leaves infected by Pseudomonas syringae pv. tabaci (Pst). The results showed that Pst infection reduced the chlorophyll content and the activities of PSII and PSI, which inhibited the normal photosynthesis of tobacco leaves. However, pretreatment with WV alleviated the degradation of chlorophyll. Treatment with WV alleviated the downregulation of core gene expression in PSII and PSI and improved the photosynthetic electron transfer in Pst-infected leaves. The levels of expression of PetE, ATPa and ATPc in the Pst-infected leaves were significantly upregulated when pretreated with WV. Pst infection increased the rate of production of superoxide anions and the contents of hydrogen peroxide. WV pretreatment could eliminate the oxidative damage of Pst-infected leaves by enhancing the activities of peroxidase (POD) and glutathione peroxidase (GPx) and upregulating the levels of expression of the POD2 and GPX2 genes. In conclusion, pretreatment with WV can alleviate the photosynthetic inhibition and oxidative damage of tobacco leaves caused by Pst infection.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46660616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-21DOI: 10.1080/17429145.2022.2102259
G. Montanaro, N. Briglia, L. Lopez, D. Amato, F. Panara, A. Petrozza, F. Cellini, V. Nuzzo
ABSTRACT Aiding optimal plant–environment interaction would favor plant resilience against environmental constrains including salt stress. We test the hypothesis that 6-Benzylaminopurine (BAP) primes grapevine’s salt tolerance in vines (Vitis vinifera) received salt water (NaCl 100 mM) through the modulation of gene expression of BAP (AHK4, AHP1) and salt-stress (CAT, APX) inducible genes and morpho-physiological traits. A subgroup of vines had previously (48 h) been primed with BAP (80 mg/L) before salt stress. The gene expressions were 30% (CAT) and 56% (APX) lower in primed salt-stressed vines than that in un-primed. Salt treatment did not increase leaf Na+ but it lowered stomatal conductance (g s), photosynthesis (A), stem water potential (less negative) and photosystem-II efficiency (F v/F m). Chlorophyll-a concentrations were 30% higher in BAP-primed compared to un-primed. Adverse effects of salt were significantly reduced, maintaining high A/g s, F v/F m and growth. After the relief of the stress, the BAP primed vines had a fast recovery.
{"title":"A synthetic cytokinin primes photosynthetic and growth response in grapevine under ion-independent salinity stress","authors":"G. Montanaro, N. Briglia, L. Lopez, D. Amato, F. Panara, A. Petrozza, F. Cellini, V. Nuzzo","doi":"10.1080/17429145.2022.2102259","DOIUrl":"https://doi.org/10.1080/17429145.2022.2102259","url":null,"abstract":"ABSTRACT Aiding optimal plant–environment interaction would favor plant resilience against environmental constrains including salt stress. We test the hypothesis that 6-Benzylaminopurine (BAP) primes grapevine’s salt tolerance in vines (Vitis vinifera) received salt water (NaCl 100 mM) through the modulation of gene expression of BAP (AHK4, AHP1) and salt-stress (CAT, APX) inducible genes and morpho-physiological traits. A subgroup of vines had previously (48 h) been primed with BAP (80 mg/L) before salt stress. The gene expressions were 30% (CAT) and 56% (APX) lower in primed salt-stressed vines than that in un-primed. Salt treatment did not increase leaf Na+ but it lowered stomatal conductance (g s), photosynthesis (A), stem water potential (less negative) and photosystem-II efficiency (F v/F m). Chlorophyll-a concentrations were 30% higher in BAP-primed compared to un-primed. Adverse effects of salt were significantly reduced, maintaining high A/g s, F v/F m and growth. After the relief of the stress, the BAP primed vines had a fast recovery.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45462752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-18DOI: 10.1080/17429145.2022.2101700
Changqian Quan, Fan Wei, Su-Hang Huang, Kun-hua Wei, Shimin Chen, J. Miao, Danfeng Tang
ABSTRACT To study the effects of light quality and salt stress on RNA editing of Mesona chinensis Benth (MCB) chloroplast genome, the RNA editing sites in the MCB chloroplast protein-coding genes were predicted and then partially verified by PCR and RT-PCR. Meanwhile, the RNA editing efficiency and relative expression of accD, ndhB, ndhF, and rpoB under red and blue light and salt stress conditions were analyzed. A total of 45 editing sites were predicted and all the editing sites were C-to-U conversion. 12 predicted editing sites were verified. The expression level of accD was down-regulated under red light compared with the blue light, as well as down-regulated under salt stress compared with the normal condition (CK). Additionally, the editing efficiency of accD-287 was 96.7% under normal condition, higher than that under salt stress (93.3%) but lower than those under blue and red light (both 100%). In ndhB, ndhB-494 was partially edited under normal growth condition but completely edited under blue and red light and salt stress, and other sites were completely edited under all conditions. It was indicated that the editing frequency was not positively relevant to the transcript level. Besides, accD-287 and ndhB-494 might be involved in response to salt stress.
{"title":"RNA editing analysis of some chloroplast transcripts and its response to light and salt stress in Mesona chinensis Benth","authors":"Changqian Quan, Fan Wei, Su-Hang Huang, Kun-hua Wei, Shimin Chen, J. Miao, Danfeng Tang","doi":"10.1080/17429145.2022.2101700","DOIUrl":"https://doi.org/10.1080/17429145.2022.2101700","url":null,"abstract":"ABSTRACT To study the effects of light quality and salt stress on RNA editing of Mesona chinensis Benth (MCB) chloroplast genome, the RNA editing sites in the MCB chloroplast protein-coding genes were predicted and then partially verified by PCR and RT-PCR. Meanwhile, the RNA editing efficiency and relative expression of accD, ndhB, ndhF, and rpoB under red and blue light and salt stress conditions were analyzed. A total of 45 editing sites were predicted and all the editing sites were C-to-U conversion. 12 predicted editing sites were verified. The expression level of accD was down-regulated under red light compared with the blue light, as well as down-regulated under salt stress compared with the normal condition (CK). Additionally, the editing efficiency of accD-287 was 96.7% under normal condition, higher than that under salt stress (93.3%) but lower than those under blue and red light (both 100%). In ndhB, ndhB-494 was partially edited under normal growth condition but completely edited under blue and red light and salt stress, and other sites were completely edited under all conditions. It was indicated that the editing frequency was not positively relevant to the transcript level. Besides, accD-287 and ndhB-494 might be involved in response to salt stress.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42998799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Heavy metal (HM) pollution is increasingly becoming a serious threat to public and environmental health with more-than-ever rapid industrialization and urbanization activities. Phytoremediation is a sustainable and largely accepted technology because of its low cost, simple operation, environmental safety and recognized as a promising approach for environmental remediation applications. Hyperaccumulator plants are the core of phytoremediation, and the study of their accumulation, detoxification, and HM tolerance mechanisms is fundamental to the progress of phytoremediation. In-depth investigations to understand the physiochemical and dissipation pathways of hyperaccumulators such as Leersia hexandra Swartz (L. hexandra) which can serve as a useful tool in environmental remediation applications. L. hexandra as a chromium hyperaccumulator plant, can also be a remarkable choice to remediate copper and nickel contaminated soils. Therefore, this article summarizes the previous studies on the detoxification strategies/tolerance mechanisms and the enhancement of the properties of L. hexandra, which will inspire its future applications in the sustainable environmental cleanup initiatives.
{"title":"Advances in heavy metals detoxification, tolerance, accumulation mechanisms, and properties enhancement of Leersia hexandra Swartz","authors":"Mouyixing Chen, Xue-hong Zhang, P. Jiang, Jiun-Cheng Liu, Shaohong You, Youran Lv","doi":"10.1080/17429145.2022.2096266","DOIUrl":"https://doi.org/10.1080/17429145.2022.2096266","url":null,"abstract":"ABSTRACT Heavy metal (HM) pollution is increasingly becoming a serious threat to public and environmental health with more-than-ever rapid industrialization and urbanization activities. Phytoremediation is a sustainable and largely accepted technology because of its low cost, simple operation, environmental safety and recognized as a promising approach for environmental remediation applications. Hyperaccumulator plants are the core of phytoremediation, and the study of their accumulation, detoxification, and HM tolerance mechanisms is fundamental to the progress of phytoremediation. In-depth investigations to understand the physiochemical and dissipation pathways of hyperaccumulators such as Leersia hexandra Swartz (L. hexandra) which can serve as a useful tool in environmental remediation applications. L. hexandra as a chromium hyperaccumulator plant, can also be a remarkable choice to remediate copper and nickel contaminated soils. Therefore, this article summarizes the previous studies on the detoxification strategies/tolerance mechanisms and the enhancement of the properties of L. hexandra, which will inspire its future applications in the sustainable environmental cleanup initiatives.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45531131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-07DOI: 10.1080/17429145.2022.2089752
Li Liu, Mengjiao An, Xiu-jie Li, Zhen Han, Shao-xuan Li, Bo Li
ABSTRACT Nitrogen (N) in different forms has been demonstrated to play significant roles in plants. However, little is known about molybdenum (Mo) effects on N absorption and utilization in grapevine seedlings grown under different N sources. The present study used a sand culture system to analyze the impact of Mo application (0 μM; 1 μM) on N absorption and utilization in grapevine (Vitislabrusca × V. vinifera ‘Shine Muscat’ (rootstock 3309 m)) young potted seedlings under different N sources (NO3 −, NH4NO3 and NH4 +). The different N forms and Mo application significantly influenced dry matter accumulation, and root architecture and activity. The effects of Mo on total N content followed the order of (NH4NO3 > NO3 − > NH4 +). Moreover, Mo and N induced VvMOT1 and VvNRT1.1 expression synergistically. Mo supply altered the utilization of NO3 −, NO2 −, and NH4 + in grapevines under different N sources. NH4NO3 showed the highest effect while NH4 + the least. Furthermore, the 15N-labeling experiment showed that the 15N content in shoot and root and the 15N-use efficiency were the highest after Mo application under NH4NO3 source, indicating the synergistic effects of Mo with the co-application of NO3 − and NH4 + sources. The study’s findings provide insights on Mo and N fertilizer utilization for cultivation and production practices in fruits.
{"title":"Molybdenum-induced effects on nitrogen absorption and utilization under different nitrogen sources in Vitis vinifera","authors":"Li Liu, Mengjiao An, Xiu-jie Li, Zhen Han, Shao-xuan Li, Bo Li","doi":"10.1080/17429145.2022.2089752","DOIUrl":"https://doi.org/10.1080/17429145.2022.2089752","url":null,"abstract":"ABSTRACT\u0000 Nitrogen (N) in different forms has been demonstrated to play significant roles in plants. However, little is known about molybdenum (Mo) effects on N absorption and utilization in grapevine seedlings grown under different N sources. The present study used a sand culture system to analyze the impact of Mo application (0 μM; 1 μM) on N absorption and utilization in grapevine (Vitislabrusca × V. vinifera ‘Shine Muscat’ (rootstock 3309 m)) young potted seedlings under different N sources (NO3 −, NH4NO3 and NH4 +). The different N forms and Mo application significantly influenced dry matter accumulation, and root architecture and activity. The effects of Mo on total N content followed the order of (NH4NO3 > NO3 − > NH4 +). Moreover, Mo and N induced VvMOT1 and VvNRT1.1 expression synergistically. Mo supply altered the utilization of NO3 −, NO2 −, and NH4 + in grapevines under different N sources. NH4NO3 showed the highest effect while NH4 + the least. Furthermore, the 15N-labeling experiment showed that the 15N content in shoot and root and the 15N-use efficiency were the highest after Mo application under NH4NO3 source, indicating the synergistic effects of Mo with the co-application of NO3 − and NH4 + sources. The study’s findings provide insights on Mo and N fertilizer utilization for cultivation and production practices in fruits.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43204374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-04DOI: 10.1080/17429145.2022.2095449
Yanli Du, Qiang Zhao, Weijia Li, Jing Geng, Siqi Li, Xiankai Yuan, Yanhua Gu, Jingwen Zhong, Yuxian Zhang, Jidao Du
ABSTRACT Lateral organ boundary Domain (LBD) proteins are plant-specific transcription factors that play a key role in plant lateral organ development and stress tolerance. However, LBD gene has not been identified in the common bean (Phaseolus vulgaris L.). Here, a total of 47 common bean LBD genes (PvLBDs) were identified. Members of the same subfamily had similar genetic structures. Synteny analysis indicated that LBDs in the common bean genome have greater collinearity with soybean (Glycine max L.) than with Arabidopsis and rice (Oryza sativa L.). Additionally, 9 pair of segmental duplication genes were identified by collinearity analysis. Phytozome data analysis showed significant differences in PvLBD gene expression abundance between different developmental stages of the same tissue. The qRT-PCR results showed that NaCl, CdCl2, and HgCl2 stresses up-regulated 19% and down-regulated 81% of the PvLBD genes. This study provides a basis for further analysis of the function of the PvLBD gene family.
摘要侧方器官边界结构域（LBD）蛋白是一种植物特异性转录因子，在植物侧方器官发育和抗逆性中起着关键作用。然而，在普通菜豆（Phaseolus vulgaris L.）中尚未鉴定出LBD基因。本文共鉴定出47个普通菜豆LBD基因（PvLBD）。同一亚科的成员具有相似的遗传结构。Synteny分析表明，普通大豆基因组中的LBD与大豆（Glycine max L.）的共线性大于与拟南芥（Arabidopsis）和水稻（Oryza sativa L.）的同线性。Phytozome数据分析显示，同一组织不同发育阶段的PvLBD基因表达丰度存在显著差异。qRT-PCR结果显示，NaCl、CdCl2和HgCl2胁迫上调和下调了19%的PvLBD基因。本研究为进一步分析PvLBD基因家族的功能提供了基础。
{"title":"Genome-wide identification of the LBD transcription factor genes in common bean (Phaseolus vulgaris L.) and expression analysis under different abiotic stresses","authors":"Yanli Du, Qiang Zhao, Weijia Li, Jing Geng, Siqi Li, Xiankai Yuan, Yanhua Gu, Jingwen Zhong, Yuxian Zhang, Jidao Du","doi":"10.1080/17429145.2022.2095449","DOIUrl":"https://doi.org/10.1080/17429145.2022.2095449","url":null,"abstract":"ABSTRACT Lateral organ boundary Domain (LBD) proteins are plant-specific transcription factors that play a key role in plant lateral organ development and stress tolerance. However, LBD gene has not been identified in the common bean (Phaseolus vulgaris L.). Here, a total of 47 common bean LBD genes (PvLBDs) were identified. Members of the same subfamily had similar genetic structures. Synteny analysis indicated that LBDs in the common bean genome have greater collinearity with soybean (Glycine max L.) than with Arabidopsis and rice (Oryza sativa L.). Additionally, 9 pair of segmental duplication genes were identified by collinearity analysis. Phytozome data analysis showed significant differences in PvLBD gene expression abundance between different developmental stages of the same tissue. The qRT-PCR results showed that NaCl, CdCl2, and HgCl2 stresses up-regulated 19% and down-regulated 81% of the PvLBD genes. This study provides a basis for further analysis of the function of the PvLBD gene family.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47692872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-04DOI: 10.1080/17429145.2022.2094003
Rupnaz Kaur, Pankaj Kumar, Arun Kumar, Umesh Preethi Praba, Rancy Birdi, Rajveer Singh, G. Kaur, J. S. Lore, K. Neelam, Y. Vikal
ABSTRACT Sheath blight caused by Rhizoctonia solani AG1-IA is the second most serious disease of rice worldwide. Elucidating the role of multi-drug and toxic compound extrusion (MATE) gene family in host-pathogens interactions may uncover a new possible way to comprehend the mechanism of sheath blight resistance in rice. We foremost explored the role of OsMATE genes against R. solani resistance through comparative transcriptomics in PR114 (susceptible) and ShB-8 (moderately resistant) at 24 and 48 hpi (hours post-inoculation) of R. solani infection, respectively. Six OsMATE genes were differentially expressed and further validated through qRT-PCR. OsMATE6 gene was identified as a potential candidate for sheath blight susceptibility as it was significantly up-regulated in PR114. OsMATE6 is conserved within the wild relatives and might be translocated from Oryza nivara during the domestication of rice. Further studies are focused to verify its role by overexpression and protein interactions to understand the molecular mechanism of sheath blight resistance.
{"title":"OsMATE6 gene putatively involved in host defense response toward susceptibility against Rhizoctonia solani in rice","authors":"Rupnaz Kaur, Pankaj Kumar, Arun Kumar, Umesh Preethi Praba, Rancy Birdi, Rajveer Singh, G. Kaur, J. S. Lore, K. Neelam, Y. Vikal","doi":"10.1080/17429145.2022.2094003","DOIUrl":"https://doi.org/10.1080/17429145.2022.2094003","url":null,"abstract":"ABSTRACT Sheath blight caused by Rhizoctonia solani AG1-IA is the second most serious disease of rice worldwide. Elucidating the role of multi-drug and toxic compound extrusion (MATE) gene family in host-pathogens interactions may uncover a new possible way to comprehend the mechanism of sheath blight resistance in rice. We foremost explored the role of OsMATE genes against R. solani resistance through comparative transcriptomics in PR114 (susceptible) and ShB-8 (moderately resistant) at 24 and 48 hpi (hours post-inoculation) of R. solani infection, respectively. Six OsMATE genes were differentially expressed and further validated through qRT-PCR. OsMATE6 gene was identified as a potential candidate for sheath blight susceptibility as it was significantly up-regulated in PR114. OsMATE6 is conserved within the wild relatives and might be translocated from Oryza nivara during the domestication of rice. Further studies are focused to verify its role by overexpression and protein interactions to understand the molecular mechanism of sheath blight resistance.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42413450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.1080/17429145.2022.2091802
Slavica Kerečki, I. Pećinar, Vera Karličić, N. Mirković, I. Kljujev, V. Raičević, Jelena Jovičić-Petrović
ABSTRACT This study assesses the effects of Azotobacter biopriming on the early development of sugar beet. Azotobacter chroococcum F8/2 was screened for plant growth promoting characteristics and biopriming effects were estimated through germination parameters and the structural changes of the root tissues. A. chroococcum F8/2 was characterized as a contributor to nitrogen, iron, and potassium availability, as well as a producer of auxin and 1-aminocyclopropane-1-carboxilic acid deaminase. Applied biopriming had reduced mean germination time by 34.44% and increased vigor I by 90.99% compared to control. Volatile blend comprised 47.67% ethanol, 32.01% 2-methyl-propanol, 17.32% 3-methyl-1-butanol, and a trace of 2,3-butanedione. Root micromorphological analysis of bioprimed sugar beet revealed a considerable increase in primary, secondary xylem area, and vessels size. Obtained results determine A. chroococcum F8/2 as a successful biopriming agent, and active participant in nutrient availability and hormonal status modulation affecting root vascular tissue.
{"title":"Azotobacter chroococcum F8/2: a multitasking bacterial strain in sugar beet biopriming","authors":"Slavica Kerečki, I. Pećinar, Vera Karličić, N. Mirković, I. Kljujev, V. Raičević, Jelena Jovičić-Petrović","doi":"10.1080/17429145.2022.2091802","DOIUrl":"https://doi.org/10.1080/17429145.2022.2091802","url":null,"abstract":"ABSTRACT This study assesses the effects of Azotobacter biopriming on the early development of sugar beet. Azotobacter chroococcum F8/2 was screened for plant growth promoting characteristics and biopriming effects were estimated through germination parameters and the structural changes of the root tissues. A. chroococcum F8/2 was characterized as a contributor to nitrogen, iron, and potassium availability, as well as a producer of auxin and 1-aminocyclopropane-1-carboxilic acid deaminase. Applied biopriming had reduced mean germination time by 34.44% and increased vigor I by 90.99% compared to control. Volatile blend comprised 47.67% ethanol, 32.01% 2-methyl-propanol, 17.32% 3-methyl-1-butanol, and a trace of 2,3-butanedione. Root micromorphological analysis of bioprimed sugar beet revealed a considerable increase in primary, secondary xylem area, and vessels size. Obtained results determine A. chroococcum F8/2 as a successful biopriming agent, and active participant in nutrient availability and hormonal status modulation affecting root vascular tissue.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42876331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-22DOI: 10.1080/17429145.2022.2091801
Sajid Ali, Y. Moon, M. Hamayun, M. Khan, K. Bibi, In-Jung Lee
ABSTRACT Abiotic stresses lead to excessive crop yield losses and are a major threat to agriculture. It is essential to equip crops with multi-stress tolerance to mitigate the adverse effects of abiotic stressors and meet the demands of the increasing global population. The association between plants and symbiotic microorganisms is involved in key functions at the ecosystem and plant levels, and the application of microbial plant biostimulants (MPBs) is a sustainable strategy to augment plant growth and productivity, even under abiotic stress conditions. Several different microorganisms can be used as MPBs to enhance plant growth and produce progressive and reproducible effects on crops. In the present review, we assessed the current knowledge on the use of MPBs, discuss the diversity and characteristics of MPBs, and provide a meticulous assessment of the possible applications of MPBs in abiotic stress relief in crops.
{"title":"Pragmatic role of microbial plant biostimulants in abiotic stress relief in crop plants","authors":"Sajid Ali, Y. Moon, M. Hamayun, M. Khan, K. Bibi, In-Jung Lee","doi":"10.1080/17429145.2022.2091801","DOIUrl":"https://doi.org/10.1080/17429145.2022.2091801","url":null,"abstract":"ABSTRACT Abiotic stresses lead to excessive crop yield losses and are a major threat to agriculture. It is essential to equip crops with multi-stress tolerance to mitigate the adverse effects of abiotic stressors and meet the demands of the increasing global population. The association between plants and symbiotic microorganisms is involved in key functions at the ecosystem and plant levels, and the application of microbial plant biostimulants (MPBs) is a sustainable strategy to augment plant growth and productivity, even under abiotic stress conditions. Several different microorganisms can be used as MPBs to enhance plant growth and produce progressive and reproducible effects on crops. In the present review, we assessed the current knowledge on the use of MPBs, discuss the diversity and characteristics of MPBs, and provide a meticulous assessment of the possible applications of MPBs in abiotic stress relief in crops.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42748571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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