Pub Date : 2022-07-04DOI: 10.1080/17429145.2022.2095449
Yanli Du, Qiang Zhao, Weijia Li, Jing Geng, Siqi Li, Xiankai Yuan, Yanhua Gu, Jingwen Zhong, Yuxian Zhang, Jidao Du
ABSTRACT Lateral organ boundary Domain (LBD) proteins are plant-specific transcription factors that play a key role in plant lateral organ development and stress tolerance. However, LBD gene has not been identified in the common bean (Phaseolus vulgaris L.). Here, a total of 47 common bean LBD genes (PvLBDs) were identified. Members of the same subfamily had similar genetic structures. Synteny analysis indicated that LBDs in the common bean genome have greater collinearity with soybean (Glycine max L.) than with Arabidopsis and rice (Oryza sativa L.). Additionally, 9 pair of segmental duplication genes were identified by collinearity analysis. Phytozome data analysis showed significant differences in PvLBD gene expression abundance between different developmental stages of the same tissue. The qRT-PCR results showed that NaCl, CdCl2, and HgCl2 stresses up-regulated 19% and down-regulated 81% of the PvLBD genes. This study provides a basis for further analysis of the function of the PvLBD gene family.
摘要侧方器官边界结构域(LBD)蛋白是一种植物特异性转录因子,在植物侧方器官发育和抗逆性中起着关键作用。然而,在普通菜豆(Phaseolus vulgaris L.)中尚未鉴定出LBD基因。本文共鉴定出47个普通菜豆LBD基因(PvLBD)。同一亚科的成员具有相似的遗传结构。Synteny分析表明,普通大豆基因组中的LBD与大豆(Glycine max L.)的共线性大于与拟南芥(Arabidopsis)和水稻(Oryza sativa L.)的同线性。Phytozome数据分析显示,同一组织不同发育阶段的PvLBD基因表达丰度存在显著差异。qRT-PCR结果显示,NaCl、CdCl2和HgCl2胁迫上调和下调了19%的PvLBD基因。本研究为进一步分析PvLBD基因家族的功能提供了基础。
{"title":"Genome-wide identification of the LBD transcription factor genes in common bean (Phaseolus vulgaris L.) and expression analysis under different abiotic stresses","authors":"Yanli Du, Qiang Zhao, Weijia Li, Jing Geng, Siqi Li, Xiankai Yuan, Yanhua Gu, Jingwen Zhong, Yuxian Zhang, Jidao Du","doi":"10.1080/17429145.2022.2095449","DOIUrl":"https://doi.org/10.1080/17429145.2022.2095449","url":null,"abstract":"ABSTRACT Lateral organ boundary Domain (LBD) proteins are plant-specific transcription factors that play a key role in plant lateral organ development and stress tolerance. However, LBD gene has not been identified in the common bean (Phaseolus vulgaris L.). Here, a total of 47 common bean LBD genes (PvLBDs) were identified. Members of the same subfamily had similar genetic structures. Synteny analysis indicated that LBDs in the common bean genome have greater collinearity with soybean (Glycine max L.) than with Arabidopsis and rice (Oryza sativa L.). Additionally, 9 pair of segmental duplication genes were identified by collinearity analysis. Phytozome data analysis showed significant differences in PvLBD gene expression abundance between different developmental stages of the same tissue. The qRT-PCR results showed that NaCl, CdCl2, and HgCl2 stresses up-regulated 19% and down-regulated 81% of the PvLBD genes. This study provides a basis for further analysis of the function of the PvLBD gene family.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"731 - 743"},"PeriodicalIF":3.2,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47692872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-04DOI: 10.1080/17429145.2022.2094003
Rupnaz Kaur, Pankaj Kumar, Arun Kumar, Umesh Preethi Praba, Rancy Birdi, Rajveer Singh, G. Kaur, J. S. Lore, K. Neelam, Y. Vikal
ABSTRACT Sheath blight caused by Rhizoctonia solani AG1-IA is the second most serious disease of rice worldwide. Elucidating the role of multi-drug and toxic compound extrusion (MATE) gene family in host-pathogens interactions may uncover a new possible way to comprehend the mechanism of sheath blight resistance in rice. We foremost explored the role of OsMATE genes against R. solani resistance through comparative transcriptomics in PR114 (susceptible) and ShB-8 (moderately resistant) at 24 and 48 hpi (hours post-inoculation) of R. solani infection, respectively. Six OsMATE genes were differentially expressed and further validated through qRT-PCR. OsMATE6 gene was identified as a potential candidate for sheath blight susceptibility as it was significantly up-regulated in PR114. OsMATE6 is conserved within the wild relatives and might be translocated from Oryza nivara during the domestication of rice. Further studies are focused to verify its role by overexpression and protein interactions to understand the molecular mechanism of sheath blight resistance.
{"title":"OsMATE6 gene putatively involved in host defense response toward susceptibility against Rhizoctonia solani in rice","authors":"Rupnaz Kaur, Pankaj Kumar, Arun Kumar, Umesh Preethi Praba, Rancy Birdi, Rajveer Singh, G. Kaur, J. S. Lore, K. Neelam, Y. Vikal","doi":"10.1080/17429145.2022.2094003","DOIUrl":"https://doi.org/10.1080/17429145.2022.2094003","url":null,"abstract":"ABSTRACT Sheath blight caused by Rhizoctonia solani AG1-IA is the second most serious disease of rice worldwide. Elucidating the role of multi-drug and toxic compound extrusion (MATE) gene family in host-pathogens interactions may uncover a new possible way to comprehend the mechanism of sheath blight resistance in rice. We foremost explored the role of OsMATE genes against R. solani resistance through comparative transcriptomics in PR114 (susceptible) and ShB-8 (moderately resistant) at 24 and 48 hpi (hours post-inoculation) of R. solani infection, respectively. Six OsMATE genes were differentially expressed and further validated through qRT-PCR. OsMATE6 gene was identified as a potential candidate for sheath blight susceptibility as it was significantly up-regulated in PR114. OsMATE6 is conserved within the wild relatives and might be translocated from Oryza nivara during the domestication of rice. Further studies are focused to verify its role by overexpression and protein interactions to understand the molecular mechanism of sheath blight resistance.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"744 - 755"},"PeriodicalIF":3.2,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42413450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.1080/17429145.2022.2091802
Slavica Kerečki, I. Pećinar, Vera Karličić, N. Mirković, I. Kljujev, V. Raičević, Jelena Jovičić-Petrović
ABSTRACT This study assesses the effects of Azotobacter biopriming on the early development of sugar beet. Azotobacter chroococcum F8/2 was screened for plant growth promoting characteristics and biopriming effects were estimated through germination parameters and the structural changes of the root tissues. A. chroococcum F8/2 was characterized as a contributor to nitrogen, iron, and potassium availability, as well as a producer of auxin and 1-aminocyclopropane-1-carboxilic acid deaminase. Applied biopriming had reduced mean germination time by 34.44% and increased vigor I by 90.99% compared to control. Volatile blend comprised 47.67% ethanol, 32.01% 2-methyl-propanol, 17.32% 3-methyl-1-butanol, and a trace of 2,3-butanedione. Root micromorphological analysis of bioprimed sugar beet revealed a considerable increase in primary, secondary xylem area, and vessels size. Obtained results determine A. chroococcum F8/2 as a successful biopriming agent, and active participant in nutrient availability and hormonal status modulation affecting root vascular tissue.
{"title":"Azotobacter chroococcum F8/2: a multitasking bacterial strain in sugar beet biopriming","authors":"Slavica Kerečki, I. Pećinar, Vera Karličić, N. Mirković, I. Kljujev, V. Raičević, Jelena Jovičić-Petrović","doi":"10.1080/17429145.2022.2091802","DOIUrl":"https://doi.org/10.1080/17429145.2022.2091802","url":null,"abstract":"ABSTRACT This study assesses the effects of Azotobacter biopriming on the early development of sugar beet. Azotobacter chroococcum F8/2 was screened for plant growth promoting characteristics and biopriming effects were estimated through germination parameters and the structural changes of the root tissues. A. chroococcum F8/2 was characterized as a contributor to nitrogen, iron, and potassium availability, as well as a producer of auxin and 1-aminocyclopropane-1-carboxilic acid deaminase. Applied biopriming had reduced mean germination time by 34.44% and increased vigor I by 90.99% compared to control. Volatile blend comprised 47.67% ethanol, 32.01% 2-methyl-propanol, 17.32% 3-methyl-1-butanol, and a trace of 2,3-butanedione. Root micromorphological analysis of bioprimed sugar beet revealed a considerable increase in primary, secondary xylem area, and vessels size. Obtained results determine A. chroococcum F8/2 as a successful biopriming agent, and active participant in nutrient availability and hormonal status modulation affecting root vascular tissue.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"719 - 730"},"PeriodicalIF":3.2,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42876331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-22DOI: 10.1080/17429145.2022.2091801
Sajid Ali, Y. Moon, M. Hamayun, M. Khan, K. Bibi, In-Jung Lee
ABSTRACT Abiotic stresses lead to excessive crop yield losses and are a major threat to agriculture. It is essential to equip crops with multi-stress tolerance to mitigate the adverse effects of abiotic stressors and meet the demands of the increasing global population. The association between plants and symbiotic microorganisms is involved in key functions at the ecosystem and plant levels, and the application of microbial plant biostimulants (MPBs) is a sustainable strategy to augment plant growth and productivity, even under abiotic stress conditions. Several different microorganisms can be used as MPBs to enhance plant growth and produce progressive and reproducible effects on crops. In the present review, we assessed the current knowledge on the use of MPBs, discuss the diversity and characteristics of MPBs, and provide a meticulous assessment of the possible applications of MPBs in abiotic stress relief in crops.
{"title":"Pragmatic role of microbial plant biostimulants in abiotic stress relief in crop plants","authors":"Sajid Ali, Y. Moon, M. Hamayun, M. Khan, K. Bibi, In-Jung Lee","doi":"10.1080/17429145.2022.2091801","DOIUrl":"https://doi.org/10.1080/17429145.2022.2091801","url":null,"abstract":"ABSTRACT Abiotic stresses lead to excessive crop yield losses and are a major threat to agriculture. It is essential to equip crops with multi-stress tolerance to mitigate the adverse effects of abiotic stressors and meet the demands of the increasing global population. The association between plants and symbiotic microorganisms is involved in key functions at the ecosystem and plant levels, and the application of microbial plant biostimulants (MPBs) is a sustainable strategy to augment plant growth and productivity, even under abiotic stress conditions. Several different microorganisms can be used as MPBs to enhance plant growth and produce progressive and reproducible effects on crops. In the present review, we assessed the current knowledge on the use of MPBs, discuss the diversity and characteristics of MPBs, and provide a meticulous assessment of the possible applications of MPBs in abiotic stress relief in crops.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"705 - 718"},"PeriodicalIF":3.2,"publicationDate":"2022-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42748571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Cu and Zn are common and potentially harmful heavy metals to plants, animals and humans. Herein, we investigated the effects of Cu and Zn stress on the photosynthesis and tolerance mechanism of alfalfa plants to ROS using fluorescence and biochemical methods. The results showed that Cu stress significantly reduced the chlorophyll content of the leaves, while Zn stress only reduced the Chl a content. The F v/F m decreased significantly under Cu stress but was not affected by Zn treatment. However, the PI ABS of the leaves were sensitive to Cu and Zn stress. Both Cu and Zn stress resulted in the weakening of the ability of PQ library to accept electrons, the damage of OEC and the inhibition of the electron transfer from QA - to QB. Moreover, Cu stress also dissociated the thylakoids of leaves, but Zn stress did not significantly damage it. In Cu and Zn stressed leaves, the reduction of RC/CS m significantly increased the ABS/RC and TR o/RC values. When the stress intensified, the value of DI o/RC increased indicated a plant self-protection mechanism that eliminates excess energy in the PSII reaction center and increases the energy for heat dissipation per unit reaction center. Cu stress significantly increased the O2 - production rate, H2O2 content, and MDA accumulation in the leaves. However, Zn stress exhibited a minimal effect on the ROS production and oxidative damage in the alfalfa leaves but increased the O2 - production rate at the concentration of 800 μmol·L−1. Cu stress increased the activities of SOD, POD, CAT, APX, and GPX in the leaves; however, leaves adapts to Zn stress by enhancing the activities of SOD and GPX. Thus under Cu stress, the degree of photoinhibition and oxidative damage in alfalfa leaves were significantly higher than under Zn stress.
{"title":"Cu and Zn Stress affect the photosynthetic and antioxidative systems of alfalfa (Medicago sativa)","authors":"Hongzhi Chen, Linlin Song, Hongbo Zhang, Jiechen Wang, Yue Wang, Huihui Zhang","doi":"10.1080/17429145.2022.2074157","DOIUrl":"https://doi.org/10.1080/17429145.2022.2074157","url":null,"abstract":"ABSTRACT Cu and Zn are common and potentially harmful heavy metals to plants, animals and humans. Herein, we investigated the effects of Cu and Zn stress on the photosynthesis and tolerance mechanism of alfalfa plants to ROS using fluorescence and biochemical methods. The results showed that Cu stress significantly reduced the chlorophyll content of the leaves, while Zn stress only reduced the Chl a content. The F v/F m decreased significantly under Cu stress but was not affected by Zn treatment. However, the PI ABS of the leaves were sensitive to Cu and Zn stress. Both Cu and Zn stress resulted in the weakening of the ability of PQ library to accept electrons, the damage of OEC and the inhibition of the electron transfer from QA - to QB. Moreover, Cu stress also dissociated the thylakoids of leaves, but Zn stress did not significantly damage it. In Cu and Zn stressed leaves, the reduction of RC/CS m significantly increased the ABS/RC and TR o/RC values. When the stress intensified, the value of DI o/RC increased indicated a plant self-protection mechanism that eliminates excess energy in the PSII reaction center and increases the energy for heat dissipation per unit reaction center. Cu stress significantly increased the O2 - production rate, H2O2 content, and MDA accumulation in the leaves. However, Zn stress exhibited a minimal effect on the ROS production and oxidative damage in the alfalfa leaves but increased the O2 - production rate at the concentration of 800 μmol·L−1. Cu stress increased the activities of SOD, POD, CAT, APX, and GPX in the leaves; however, leaves adapts to Zn stress by enhancing the activities of SOD and GPX. Thus under Cu stress, the degree of photoinhibition and oxidative damage in alfalfa leaves were significantly higher than under Zn stress.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"695 - 704"},"PeriodicalIF":3.2,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60093788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-16DOI: 10.1080/17429145.2022.2086307
Xiu Zhang, Jingfan Yan, M. Khashi u Rahman, Fengzhi Wu
ABSTRACT Plants constantly communicate with coexisting neighbors and adjust their physiological and morphological characteristics, including changes in root system architecture (RSA). Increased or decreased biomass accumulation, horizontal and vertical asymmetric distribution are the main behavioral performances. Some evidence has shown that these performances are associated with plant plasticity such as secretion of root exudates and release of volatile organic compounds (VOCs) and describe the role of common mycorrhizal networks (CMNs) as a communication pathway during belowground interplant interaction. Here, we highlight the direct role of root exudates as cues and signals and the indirect effects via regulating soil nutrients and soil microorganisms of these media in root-root interactions on RSA have been taken into consideration. At last, the existing knowledge gaps and potential research directions have been outlined for a better understanding of plant belowground interactions via RSA.
{"title":"The impact of root exudates, volatile organic compounds, and common mycorrhizal networks on root system architecture in root-root interactions","authors":"Xiu Zhang, Jingfan Yan, M. Khashi u Rahman, Fengzhi Wu","doi":"10.1080/17429145.2022.2086307","DOIUrl":"https://doi.org/10.1080/17429145.2022.2086307","url":null,"abstract":"ABSTRACT Plants constantly communicate with coexisting neighbors and adjust their physiological and morphological characteristics, including changes in root system architecture (RSA). Increased or decreased biomass accumulation, horizontal and vertical asymmetric distribution are the main behavioral performances. Some evidence has shown that these performances are associated with plant plasticity such as secretion of root exudates and release of volatile organic compounds (VOCs) and describe the role of common mycorrhizal networks (CMNs) as a communication pathway during belowground interplant interaction. Here, we highlight the direct role of root exudates as cues and signals and the indirect effects via regulating soil nutrients and soil microorganisms of these media in root-root interactions on RSA have been taken into consideration. At last, the existing knowledge gaps and potential research directions have been outlined for a better understanding of plant belowground interactions via RSA.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"685 - 694"},"PeriodicalIF":3.2,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48926588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.1080/17429145.2022.2085335
A. Bondok, W. Mousa, Asmaa A. M. Rady, K. Saad-Allah
ABSTRACT Prolonged drought presents a serious challenge to the agricultural sector. The main concern of this work was to assess the phenotypical, physiological, and molecular responses of five teosinte (Zea mexicana Schrad L.) genotypes (Ba, Gm1, Gm2, Gm3 and Gm4). In a two-season (2020 and 2021) field experiment, fifteen-day-old teosinte plants were subjected to well-watered (15-day watering frequency) as a control, moderate drought (25-day watering frequency), and extreme drought (35-day watering frequency) treatments. Drought negatively affected growth, yield, chlorophyll, and POD activity of all genotypes, but promoted soluble sugars and proteins, osmoregulatory molecules (glycinebetaine, amino acids, and proline), non-enzymatic antioxidants (phenols, flavonoids, and alkaloids), and SOD activity. Furthermore, long-term water stress upregulated MOCOS, Rad17, NCED1, CAT1, and P5CS genes expression, with Gm3 and Gm4 being the most drought-tolerant genotypes. These findings could be employed in breeding programs to develop tolerant genotypes to address the challenges posed by climate changes like drought.
{"title":"Phenotypical, physiological and molecular assessment of drought tolerance of five Egyptian teosinte genotypes","authors":"A. Bondok, W. Mousa, Asmaa A. M. Rady, K. Saad-Allah","doi":"10.1080/17429145.2022.2085335","DOIUrl":"https://doi.org/10.1080/17429145.2022.2085335","url":null,"abstract":"ABSTRACT Prolonged drought presents a serious challenge to the agricultural sector. The main concern of this work was to assess the phenotypical, physiological, and molecular responses of five teosinte (Zea mexicana Schrad L.) genotypes (Ba, Gm1, Gm2, Gm3 and Gm4). In a two-season (2020 and 2021) field experiment, fifteen-day-old teosinte plants were subjected to well-watered (15-day watering frequency) as a control, moderate drought (25-day watering frequency), and extreme drought (35-day watering frequency) treatments. Drought negatively affected growth, yield, chlorophyll, and POD activity of all genotypes, but promoted soluble sugars and proteins, osmoregulatory molecules (glycinebetaine, amino acids, and proline), non-enzymatic antioxidants (phenols, flavonoids, and alkaloids), and SOD activity. Furthermore, long-term water stress upregulated MOCOS, Rad17, NCED1, CAT1, and P5CS genes expression, with Gm3 and Gm4 being the most drought-tolerant genotypes. These findings could be employed in breeding programs to develop tolerant genotypes to address the challenges posed by climate changes like drought.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"656 - 673"},"PeriodicalIF":3.2,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41897727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Suillus luteus is an edible ectomycorrhizal fungus (EMF). The S. luteus strain LS88 secretes many phytohormones, including salicylic acid (SA) and indole-3-carboxylic acid (ICA). LS88 was inoculated to the tree Pinus massoniana and treated with the amino acid fertilizer GGR6. Plant growth parameters, plant enzyme activities, chlorophyll contents, and element contents were analyzed. Our results show that GGR6 may help the development of S. luteus-P. massoniana ectomycorrhiza. Moreover, LS88 and GGR6 synergistically affect P. massoniana growth and element uptake. Phytohormone detection on the roots of LS88-inoculated P. massoniana seedlings showed that LS88 could significantly increase the ICA content within a week. The SA content in the roots in the inoculated group seedlings increased slightly, but the salicylic acid 2-O-β-glucoside (SAG) content decreased. Therefore, we speculate GGR6 may enhance the growth-promoting effect of EMF on plants, and LS88 affects P. massoniana growth through secreting phytohormones.
{"title":"Synergistic effect of phytohormone-producing ectomycorrhizal fungus Suillus luteus and fertilizer GGR6 on Pinus massoniana growth","authors":"Xueyu Pan, Jinhua Zhang, Zhenwen Xue, Junfeng Liang, Yanliu Chen, Ying Liu","doi":"10.1080/17429145.2022.2081369","DOIUrl":"https://doi.org/10.1080/17429145.2022.2081369","url":null,"abstract":"ABSTRACT Suillus luteus is an edible ectomycorrhizal fungus (EMF). The S. luteus strain LS88 secretes many phytohormones, including salicylic acid (SA) and indole-3-carboxylic acid (ICA). LS88 was inoculated to the tree Pinus massoniana and treated with the amino acid fertilizer GGR6. Plant growth parameters, plant enzyme activities, chlorophyll contents, and element contents were analyzed. Our results show that GGR6 may help the development of S. luteus-P. massoniana ectomycorrhiza. Moreover, LS88 and GGR6 synergistically affect P. massoniana growth and element uptake. Phytohormone detection on the roots of LS88-inoculated P. massoniana seedlings showed that LS88 could significantly increase the ICA content within a week. The SA content in the roots in the inoculated group seedlings increased slightly, but the salicylic acid 2-O-β-glucoside (SAG) content decreased. Therefore, we speculate GGR6 may enhance the growth-promoting effect of EMF on plants, and LS88 affects P. massoniana growth through secreting phytohormones.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"643 - 655"},"PeriodicalIF":3.2,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46461124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-26DOI: 10.1080/17429145.2022.2080291
Yang Zhao, Yingying He, Xixi Wang, Chang-feng Qu, J. Miao
ABSTRACT It has been discovered that many plants accumulate proline in response to osmotic stress. However, the interaction between salinity stress and proline metabolism-related genes has not been unequivocally identified in Spartina alterniflora. In our research, we successfully cloned key genes: SaP5CS1 and SaP5CS2, SaOAT, SaProT and correlated with expression profiles including SaPDH were also investigated. As the key enzyme genes for proline metabolism, the up-regulated expression of SaP5CS2 played a leading part under salt stress. Chlorophyll contents decreased gradually degree in leaves with prolonged salt stress time. Additionally, transgenic Arabidopsis overexpressing SaP5CS2 showed high tolerance to salt stress and accumulated more proline and H2O2 content was also reduced in salt stress condition. This study provided a foundation for deciphering the molecular osmoregulation underlying proline metabolism in S. alterniflora and SaP5CS2 may play an important role in conferred salinity tolerance by the regulation of proline.
{"title":"Proline metabolism regulation in Spartina alterniflora and SaP5CS2 gene positively regulates salt stress tolerance in transgenic Arabidopsis thaliana","authors":"Yang Zhao, Yingying He, Xixi Wang, Chang-feng Qu, J. Miao","doi":"10.1080/17429145.2022.2080291","DOIUrl":"https://doi.org/10.1080/17429145.2022.2080291","url":null,"abstract":"ABSTRACT It has been discovered that many plants accumulate proline in response to osmotic stress. However, the interaction between salinity stress and proline metabolism-related genes has not been unequivocally identified in Spartina alterniflora. In our research, we successfully cloned key genes: SaP5CS1 and SaP5CS2, SaOAT, SaProT and correlated with expression profiles including SaPDH were also investigated. As the key enzyme genes for proline metabolism, the up-regulated expression of SaP5CS2 played a leading part under salt stress. Chlorophyll contents decreased gradually degree in leaves with prolonged salt stress time. Additionally, transgenic Arabidopsis overexpressing SaP5CS2 showed high tolerance to salt stress and accumulated more proline and H2O2 content was also reduced in salt stress condition. This study provided a foundation for deciphering the molecular osmoregulation underlying proline metabolism in S. alterniflora and SaP5CS2 may play an important role in conferred salinity tolerance by the regulation of proline.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"632 - 642"},"PeriodicalIF":3.2,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42437625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT The DEAD-box family has been shown to play an important role in a variety of abiotic stresses, but little is known in studies of mangrove plants. Here, the effects of cold stress on various physiological changes and the role of the DEAD-box RNA helicase family in response to cold stress were determined. First, we identified 73 DEAD-box RNA helicase family members in L. littorea. Second, the evolutionary relationships between the DEAD family in L. littorea and the model species Arabidopsis thaliana were investigated by evolutionary phylogenetic analysis. Finally, qRT-PCR study of representative DEAD-box genes showed that DEAD-box genes played a major role in the low-temperature stress response of L. littorea. Furthermore, we found that LlDEAD48, LlDEAD36, and LlDEAD47 might be involved in the maintenance of chlorophyll function, and LlDEAD43 might play a role in the maintenance of mitochondrial function in L. littorea under cold stress.
{"title":"Comprehensive comparative analysis and expression profiles and effects on physiological response of DEAD-box RNA helicase genes in Lumnitzera littorea (Jack) Voigt under cold stress","authors":"Lulu Hao, Ying Zhang, Yin Li, Linxia Bai, Danfei Yue, Huiyu Zhang, Chun-ying Zheng","doi":"10.1080/17429145.2022.2074158","DOIUrl":"https://doi.org/10.1080/17429145.2022.2074158","url":null,"abstract":"ABSTRACT The DEAD-box family has been shown to play an important role in a variety of abiotic stresses, but little is known in studies of mangrove plants. Here, the effects of cold stress on various physiological changes and the role of the DEAD-box RNA helicase family in response to cold stress were determined. First, we identified 73 DEAD-box RNA helicase family members in L. littorea. Second, the evolutionary relationships between the DEAD family in L. littorea and the model species Arabidopsis thaliana were investigated by evolutionary phylogenetic analysis. Finally, qRT-PCR study of representative DEAD-box genes showed that DEAD-box genes played a major role in the low-temperature stress response of L. littorea. Furthermore, we found that LlDEAD48, LlDEAD36, and LlDEAD47 might be involved in the maintenance of chlorophyll function, and LlDEAD43 might play a role in the maintenance of mitochondrial function in L. littorea under cold stress.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"595 - 607"},"PeriodicalIF":3.2,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46301547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}