ABSTRACT Endophytic plant-growth-promoting bacteria help plants to cope with severe environmental stresses. A Klebsiella sp. strain San01 isolated from sweet potato roots exhibited diverse plant-growth-promoting activities, including indole acetic acid production, ammonia production, and phosphate solubilization. San01 inoculation significantly increased the growth characteristics of sweet potato. Moreover, the sweet potato inoculated with San01 resulted in the alleviation of drought and salinity stresses. In comparison with the non-inoculated plants, the wilting symptom and the inhibition of photochemical response were significantly diminished in San01-inoculated plants under stress conditions. In the presence of San01, the enhancement of 2,2-diphenyl-1-picrylhydrazyl radical scavenging ability and elevated activities of ascorbate peroxidase and superoxide dismutase were observed to combat the oxidative stress in sweet potato. Further investigation showed that the San01 inoculation enhanced the expression of the IbLEA gene in sweet potato under salinity and drought. These results confer a promising biotechnological approach through San01 interaction to develop stress-tolerant sweet potato plants.
{"title":"Endophytic Klebsiella sp. San01 promotes growth performance and induces salinity and drought tolerance in sweet potato (Ipomoea batatas L.)","authors":"Qing Li, Zhehong Huang, Caisheng Deng, K. Lin, Shumei Hua, Shi-Peng Chen","doi":"10.1080/17429145.2022.2077464","DOIUrl":"https://doi.org/10.1080/17429145.2022.2077464","url":null,"abstract":"ABSTRACT Endophytic plant-growth-promoting bacteria help plants to cope with severe environmental stresses. A Klebsiella sp. strain San01 isolated from sweet potato roots exhibited diverse plant-growth-promoting activities, including indole acetic acid production, ammonia production, and phosphate solubilization. San01 inoculation significantly increased the growth characteristics of sweet potato. Moreover, the sweet potato inoculated with San01 resulted in the alleviation of drought and salinity stresses. In comparison with the non-inoculated plants, the wilting symptom and the inhibition of photochemical response were significantly diminished in San01-inoculated plants under stress conditions. In the presence of San01, the enhancement of 2,2-diphenyl-1-picrylhydrazyl radical scavenging ability and elevated activities of ascorbate peroxidase and superoxide dismutase were observed to combat the oxidative stress in sweet potato. Further investigation showed that the San01 inoculation enhanced the expression of the IbLEA gene in sweet potato under salinity and drought. These results confer a promising biotechnological approach through San01 interaction to develop stress-tolerant sweet potato plants.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"608 - 619"},"PeriodicalIF":3.2,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43717342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-23DOI: 10.1080/17429145.2022.2076940
Maryam Khan, Saman Taufiq, Irum Nauman, Norina Noor, Tooba Iqbal, H. Ali, R. Z. Paracha, Faiza Munir, A. Gul, Rabia Amir
ABSTRACT Peanut (Arachis hypogaea L.) harbors a plethora of flavonoids that impart its health-promoting properties. To understand its biosynthesis and accumulation, a comparative expression analysis of flavonoid biosynthetic genes encoding enzymes responsible for synthesizing the main structure of flavonoids has been conducted in two differentially performing peanut varieties (PG 1247 and Bari 2011) in response to photoperiod and water availability. Transcript profiling of flavonoid biosynthetic genes indicated time-dependent expression of genes except for AhFLS in selected varieties in response to photoperiod variation. Many genes depicted a low degree of variation within varieties and water-deficient treatments. However, AhANS showed the highest variation for stress duration and varieties. In conclusion, this study has characterized the role of flavonoid biosynthesis in peanuts in response to photoperiod and water availability. Moreover, correlation analysis unraveled the coordination among the expression of flavonoid biosynthetic genes.
{"title":"Photoperiod and water-deficient conditions differentially regulate structural flavonoid biosynthetic genes in peanuts","authors":"Maryam Khan, Saman Taufiq, Irum Nauman, Norina Noor, Tooba Iqbal, H. Ali, R. Z. Paracha, Faiza Munir, A. Gul, Rabia Amir","doi":"10.1080/17429145.2022.2076940","DOIUrl":"https://doi.org/10.1080/17429145.2022.2076940","url":null,"abstract":"ABSTRACT Peanut (Arachis hypogaea L.) harbors a plethora of flavonoids that impart its health-promoting properties. To understand its biosynthesis and accumulation, a comparative expression analysis of flavonoid biosynthetic genes encoding enzymes responsible for synthesizing the main structure of flavonoids has been conducted in two differentially performing peanut varieties (PG 1247 and Bari 2011) in response to photoperiod and water availability. Transcript profiling of flavonoid biosynthetic genes indicated time-dependent expression of genes except for AhFLS in selected varieties in response to photoperiod variation. Many genes depicted a low degree of variation within varieties and water-deficient treatments. However, AhANS showed the highest variation for stress duration and varieties. In conclusion, this study has characterized the role of flavonoid biosynthesis in peanuts in response to photoperiod and water availability. Moreover, correlation analysis unraveled the coordination among the expression of flavonoid biosynthetic genes.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"620 - 631"},"PeriodicalIF":3.2,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47343644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-23DOI: 10.1080/17429145.2022.2065371
Anamika Sharma, A. Raman
ABSTRACT The Psylloidea, >4000 named species known today, are plant-feeding, sap-sucking insects sleeved under the Sternorrhyncha. Most species of Psylloidea are confined to the tropics. They occur as gall-inducing, free-living, and lerp-forming taxa. Lifecycles and generations of gall-inducing Psylloidea vary in temperate and tropical worlds. The Triozidae, Aphalaridae, and Calophyidae include several taxa that induce galls of diverse morphologies, from simple pits and leaf-margin rolls to complex pouches and of two-tier structures. The feeding mechanism and nutritional physiology of the gall-inducing taxa of the Psylloidea differ from those of the free-living and lerp-forming species. A majority of the gall-inducing Psylloidea are associated with the dicotyledons and a small number with the monocotyledons. The gall-inducing Psylloidea are specific to certain plants. Their host specificity is regulated by specific lipids and sterols. The gall-inducing Psylloidea show conservative behavior in terms of geographical distribution. Although the life histories of several gall-inducing Psylloidea are known today, aspects explaining their association with host plants are little known. Details of nutritional physiology of gall-inducing Psylloidea are less known presently compared with that of the free-living species. A better understanding of the association and level of relationship between gall-inducing Psylloidea and their host plants is necessary.
{"title":"Gall-inducing Psylloidea (Insecta: Hemiptera) – plant interactions","authors":"Anamika Sharma, A. Raman","doi":"10.1080/17429145.2022.2065371","DOIUrl":"https://doi.org/10.1080/17429145.2022.2065371","url":null,"abstract":"ABSTRACT The Psylloidea, >4000 named species known today, are plant-feeding, sap-sucking insects sleeved under the Sternorrhyncha. Most species of Psylloidea are confined to the tropics. They occur as gall-inducing, free-living, and lerp-forming taxa. Lifecycles and generations of gall-inducing Psylloidea vary in temperate and tropical worlds. The Triozidae, Aphalaridae, and Calophyidae include several taxa that induce galls of diverse morphologies, from simple pits and leaf-margin rolls to complex pouches and of two-tier structures. The feeding mechanism and nutritional physiology of the gall-inducing taxa of the Psylloidea differ from those of the free-living and lerp-forming species. A majority of the gall-inducing Psylloidea are associated with the dicotyledons and a small number with the monocotyledons. The gall-inducing Psylloidea are specific to certain plants. Their host specificity is regulated by specific lipids and sterols. The gall-inducing Psylloidea show conservative behavior in terms of geographical distribution. Although the life histories of several gall-inducing Psylloidea are known today, aspects explaining their association with host plants are little known. Details of nutritional physiology of gall-inducing Psylloidea are less known presently compared with that of the free-living species. A better understanding of the association and level of relationship between gall-inducing Psylloidea and their host plants is necessary.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"580 - 594"},"PeriodicalIF":3.2,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47538934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-16DOI: 10.1080/17429145.2022.2072528
Anna Maria Aprile, M. Coppola, David Turrà, S. Vitale, P. Cascone, G. Diretto, A. Fiore, Valeria Castaldi, A. Romanelli, C. Avitabile, E. Guerrieri, S. Woo, R. Rao
ABSTRACT Trichoderma spp. are among the most widely used plant beneficial fungi in agriculture. Its interaction with the plant triggers resistance responses by the activation of Induced Systemic Resistance mediated by Jasmonic acid and Ethylene and/or Systemic Acquired Resistance, which involves Salicylic acid, with the consequent control of a wide range of plant parasites. However, the benefit they can confer to plants may be reduced or nullified by environmental conditions or fungal ecological fitness. A novel approach to enhance their effectiveness in plant defense is to combine them with bioactive molecules including plant-derived compounds. Here, we show that plant treatment with Trichoderma afroharzianum (strain T22) and Systemin, a tomato peptide active in triggering plant defense, confers protection against the fungal pathogens Fusarium oxysporum, Botrytis cinerea and the insect pest Tuta absoluta. The observed defensive response was associated with an increase of Jasmonic acid and related metabolites and a decrease of Salicylic acid.
{"title":"Combination of the Systemin peptide with the beneficial fungus Trichoderma afroharzianum T22 improves plant defense responses against pests and diseases","authors":"Anna Maria Aprile, M. Coppola, David Turrà, S. Vitale, P. Cascone, G. Diretto, A. Fiore, Valeria Castaldi, A. Romanelli, C. Avitabile, E. Guerrieri, S. Woo, R. Rao","doi":"10.1080/17429145.2022.2072528","DOIUrl":"https://doi.org/10.1080/17429145.2022.2072528","url":null,"abstract":"ABSTRACT Trichoderma spp. are among the most widely used plant beneficial fungi in agriculture. Its interaction with the plant triggers resistance responses by the activation of Induced Systemic Resistance mediated by Jasmonic acid and Ethylene and/or Systemic Acquired Resistance, which involves Salicylic acid, with the consequent control of a wide range of plant parasites. However, the benefit they can confer to plants may be reduced or nullified by environmental conditions or fungal ecological fitness. A novel approach to enhance their effectiveness in plant defense is to combine them with bioactive molecules including plant-derived compounds. Here, we show that plant treatment with Trichoderma afroharzianum (strain T22) and Systemin, a tomato peptide active in triggering plant defense, confers protection against the fungal pathogens Fusarium oxysporum, Botrytis cinerea and the insect pest Tuta absoluta. The observed defensive response was associated with an increase of Jasmonic acid and related metabolites and a decrease of Salicylic acid.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"569 - 579"},"PeriodicalIF":3.2,"publicationDate":"2022-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41675709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Phytocytokines are plant peptide signals perceived by plasma membrane-localized receptors in regulating plant immunity. It was recently reported that the phytocytokine SERINE-RICH ENDOGENOUS PEPTIDE12 (SCOOP12) is recognized by the receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2) and activates plant immune responses and resistance to pathogens in Arabidopsis. Here, we show that Arabidopsis ENHANCER OF VASCULAR WILT RESISTANCE 1 (EWR1) and four EWR1 close propeptide homologs encode functional SCOOP peptides, which are able to activate immune responses via MIK2 and BRASSINOSTEROID INSENSITVE 1 (BRI1)-ASSOCIATED RECEPTOR KINASE 1 (BAK1) and SOMATIC EMBRYOGENESIS RECEPTOR KINASE 4 (SERK4).
{"title":"EWR1 as a SCOOP peptide activates MIK2-dependent immunity in Arabidopsis","authors":"J Zhang, Jinxiu Zhao, Yifei Yang, Qixin Bao, Yuxi Li, Hongbo Wang, Shuguo Hou","doi":"10.1080/17429145.2022.2070292","DOIUrl":"https://doi.org/10.1080/17429145.2022.2070292","url":null,"abstract":"ABSTRACT Phytocytokines are plant peptide signals perceived by plasma membrane-localized receptors in regulating plant immunity. It was recently reported that the phytocytokine SERINE-RICH ENDOGENOUS PEPTIDE12 (SCOOP12) is recognized by the receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2) and activates plant immune responses and resistance to pathogens in Arabidopsis. Here, we show that Arabidopsis ENHANCER OF VASCULAR WILT RESISTANCE 1 (EWR1) and four EWR1 close propeptide homologs encode functional SCOOP peptides, which are able to activate immune responses via MIK2 and BRASSINOSTEROID INSENSITVE 1 (BRI1)-ASSOCIATED RECEPTOR KINASE 1 (BAK1) and SOMATIC EMBRYOGENESIS RECEPTOR KINASE 4 (SERK4).","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"562 - 568"},"PeriodicalIF":3.2,"publicationDate":"2022-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47956246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Orobanche cumana Wallr. is the most serious parasitic weed that threatens sunflower production in China, it infects sunflower roots and causes severe yield and economic losses. This study determined the effects of O. cumana infection on sunflower growth, physiological, biochemical, photosynthesis indexes and used RNA-Seq to investigate the potential regulatory factors involved in the parasitic interaction. Results showed that O. cumana infestation significantly inhibited sunflower height, fresh weight, chlorophyl contents, photosynthetic and the MDA content, SOD and POD activities were increased in different degrees, which might be related to sunflower resistance to O. cumana infestation. Additionally, six parasite-related genes were selected, which markedly enriched in plant hormone signal transduction, photorespiration and phenylpropanoid metabolism pathway. Among these genes, HsP90A, MYC2 and HAO were discovered for the first time in sunflowers and O. cumana parasitic interaction. Undoubtedly, the results lay a foundation for revealing the parasitic interactions molecular mechanism of O. cumana and sunflowers.
{"title":"Molecular mechanism of the parasitic interaction between Orobanche cumana wallr. and sunflowers","authors":"Yixiao Zhang, Jietian Su, X. Yun, Wenlong Wu, Shouhui Wei, Zhaofeng Huang, Chaoxian Zhang, Q. Bai, Hong-juan Huang","doi":"10.1080/17429145.2022.2062061","DOIUrl":"https://doi.org/10.1080/17429145.2022.2062061","url":null,"abstract":"ABSTRACT Orobanche cumana Wallr. is the most serious parasitic weed that threatens sunflower production in China, it infects sunflower roots and causes severe yield and economic losses. This study determined the effects of O. cumana infection on sunflower growth, physiological, biochemical, photosynthesis indexes and used RNA-Seq to investigate the potential regulatory factors involved in the parasitic interaction. Results showed that O. cumana infestation significantly inhibited sunflower height, fresh weight, chlorophyl contents, photosynthetic and the MDA content, SOD and POD activities were increased in different degrees, which might be related to sunflower resistance to O. cumana infestation. Additionally, six parasite-related genes were selected, which markedly enriched in plant hormone signal transduction, photorespiration and phenylpropanoid metabolism pathway. Among these genes, HsP90A, MYC2 and HAO were discovered for the first time in sunflowers and O. cumana parasitic interaction. Undoubtedly, the results lay a foundation for revealing the parasitic interactions molecular mechanism of O. cumana and sunflowers.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"549 - 561"},"PeriodicalIF":3.2,"publicationDate":"2022-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42539705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-30DOI: 10.1080/17429145.2022.2069296
Yan Zhou, Xianglan Huang, Rui Li, Hongshi Lin, Yan Huang, Tao Zhang, Yuxing Mo, Kaidong Liu
ABSTRACT Delay the ripening can improve fruit shelf life. Reduced glutathione (GSH) is an antioxidant that delays the ripening of fruits, though the GSH-mediated mechanism involved in fruit-ripening processes is currently unclear. This study used RNA sequencing to assess the GSH-induced transcriptional and biochemical alterations observed in tomato fruit during the post-harvest process. We found 970 differentially expressed genes (DEGs) after GSH treatment, and 124 were found to be candidate genes related to the ripening of GSH-mediated fruit. In addition, the expression levels of several candidate DEGs observed in ripe tomato fruit after GSH treatments were confirmed using quantitative real-time PCR. Biochemical analyses revealed that the GSH treatment decreased the proline content and the lipid peroxidation and ascorbate peroxidase activity levels. In contrast, it increased the superoxide dismutase, peroxidase, and catalase activity levels, as well as endogenous glutathione and ascorbic acid contents. These results confirm the important role played by GSH during the process of ripening tomato fruit.
{"title":"Transcriptome and biochemical analyses of glutathione-dependent regulation of tomato fruit ripening","authors":"Yan Zhou, Xianglan Huang, Rui Li, Hongshi Lin, Yan Huang, Tao Zhang, Yuxing Mo, Kaidong Liu","doi":"10.1080/17429145.2022.2069296","DOIUrl":"https://doi.org/10.1080/17429145.2022.2069296","url":null,"abstract":"ABSTRACT Delay the ripening can improve fruit shelf life. Reduced glutathione (GSH) is an antioxidant that delays the ripening of fruits, though the GSH-mediated mechanism involved in fruit-ripening processes is currently unclear. This study used RNA sequencing to assess the GSH-induced transcriptional and biochemical alterations observed in tomato fruit during the post-harvest process. We found 970 differentially expressed genes (DEGs) after GSH treatment, and 124 were found to be candidate genes related to the ripening of GSH-mediated fruit. In addition, the expression levels of several candidate DEGs observed in ripe tomato fruit after GSH treatments were confirmed using quantitative real-time PCR. Biochemical analyses revealed that the GSH treatment decreased the proline content and the lipid peroxidation and ascorbate peroxidase activity levels. In contrast, it increased the superoxide dismutase, peroxidase, and catalase activity levels, as well as endogenous glutathione and ascorbic acid contents. These results confirm the important role played by GSH during the process of ripening tomato fruit.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"537 - 547"},"PeriodicalIF":3.2,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43978993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-27DOI: 10.1080/17429145.2022.2066730
M. Ewas, P. W. Harlina, R. Shahzad, E. Khames, F. Ali, E. Nishawy, Nagwa Elsafty, H. Ibrahim, P. Gallego
ABSTRACT Tomato is one of the major economically domesticated crops, and it is extensively used in different ways and purposes worldwide. Cell metabolism is the central core of all the biological processes to sustain life including cell growth, differentiation, maintenance, and response to environmental stress. To evaluate how genetic engineering can improve tomato fruit metabolome, the transcriptomic and metabolomic datasets of two transgenic tomatoes (SlMX1 overexpression and RNAi lines) have been compared with wild-type. The combined results demonstrated that the constitutive expression of SlMX1 not only increased trichome formation, carotenoids, and terpenoids as has been stated in several studies, but has also up- and down-regulated the expression of multiple genes related to cell growth (cell wall turnover), primary (carbohydrates, vitamins, and phytohormones), and secondary (phenylpropanoids, carotenoids, and terpenoids) metabolism, cell signaling, and stress responses. These changes in gene expression due to the constitutive expression of SlMX1 promote the most important agroeconomic traits such as fruit yield and quality, biosynthesis of health-promoting phytochemicals (including phenolic acids, flavonoids, and anthocyanins), and finally, activate resistance to Botrytis cinerea and repress the expression of over-ripening-related genes, thus extending the fruit shelf-life. In conclusion, the traits improvement achieved by SlMX1 overexpression can be harnessed in molecular breeding programs to engineer fruit size and yield, induce health-promoting secondary metabolites, promote fungal resistance, and finally extend the fruit shelf-life.
{"title":"Constitutive expression of SlMX1 gene improves fruit yield and quality, health-promoting compounds, fungal resistance and delays ripening in transgenic tomato plants","authors":"M. Ewas, P. W. Harlina, R. Shahzad, E. Khames, F. Ali, E. Nishawy, Nagwa Elsafty, H. Ibrahim, P. Gallego","doi":"10.1080/17429145.2022.2066730","DOIUrl":"https://doi.org/10.1080/17429145.2022.2066730","url":null,"abstract":"ABSTRACT Tomato is one of the major economically domesticated crops, and it is extensively used in different ways and purposes worldwide. Cell metabolism is the central core of all the biological processes to sustain life including cell growth, differentiation, maintenance, and response to environmental stress. To evaluate how genetic engineering can improve tomato fruit metabolome, the transcriptomic and metabolomic datasets of two transgenic tomatoes (SlMX1 overexpression and RNAi lines) have been compared with wild-type. The combined results demonstrated that the constitutive expression of SlMX1 not only increased trichome formation, carotenoids, and terpenoids as has been stated in several studies, but has also up- and down-regulated the expression of multiple genes related to cell growth (cell wall turnover), primary (carbohydrates, vitamins, and phytohormones), and secondary (phenylpropanoids, carotenoids, and terpenoids) metabolism, cell signaling, and stress responses. These changes in gene expression due to the constitutive expression of SlMX1 promote the most important agroeconomic traits such as fruit yield and quality, biosynthesis of health-promoting phytochemicals (including phenolic acids, flavonoids, and anthocyanins), and finally, activate resistance to Botrytis cinerea and repress the expression of over-ripening-related genes, thus extending the fruit shelf-life. In conclusion, the traits improvement achieved by SlMX1 overexpression can be harnessed in molecular breeding programs to engineer fruit size and yield, induce health-promoting secondary metabolites, promote fungal resistance, and finally extend the fruit shelf-life.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"517 - 536"},"PeriodicalIF":3.2,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44811684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-05DOI: 10.1080/17429145.2022.2056250
Yingbo Li, G. Guo, Hongwei Xu, Yingjie Zong, Shuwei Zhang, Linli Huang, R. Gao, R. Lu, Longhua Zhou, Chenghong Liu
ABSTRACT Barley (Hordeum vulgare L.) has been recognized as an ideal model plant to study the mechanism of environmental adaptation for crop improvement owing to its wide adaption to abiotic stresses. Abscisic acid is an essential hormone involved in many abiotic stresses. Here, an ABA-sensitive barley mutant (abas1) was generated by microspore embryogenesis. On exogenous ABA treatment, the seedlings of abas1 presented less plant height, root length, shoot and root dry matter than the wild type, and more genes showedigher expressional intensity and up-regulation models by RNA-Seq profiling. The gene ontology analysis revealed that the regulation of DEGs may lead to the activation of the ROS pathway and consumption of more energy in abas1, specifically. Furthermore, the up-regulation of two candidate genes (P450 and NsLTP) in abas1 may contribute to the variation of ABA sensitivity. Our findings provide more valuable material and information toward the understanding of the mechanism of ABA response in barley.
{"title":"Phenotypic and transcriptomic characterization of an ABA-sensitive mutant generated by microspore embryogenesis in barley","authors":"Yingbo Li, G. Guo, Hongwei Xu, Yingjie Zong, Shuwei Zhang, Linli Huang, R. Gao, R. Lu, Longhua Zhou, Chenghong Liu","doi":"10.1080/17429145.2022.2056250","DOIUrl":"https://doi.org/10.1080/17429145.2022.2056250","url":null,"abstract":"ABSTRACT Barley (Hordeum vulgare L.) has been recognized as an ideal model plant to study the mechanism of environmental adaptation for crop improvement owing to its wide adaption to abiotic stresses. Abscisic acid is an essential hormone involved in many abiotic stresses. Here, an ABA-sensitive barley mutant (abas1) was generated by microspore embryogenesis. On exogenous ABA treatment, the seedlings of abas1 presented less plant height, root length, shoot and root dry matter than the wild type, and more genes showedigher expressional intensity and up-regulation models by RNA-Seq profiling. The gene ontology analysis revealed that the regulation of DEGs may lead to the activation of the ROS pathway and consumption of more energy in abas1, specifically. Furthermore, the up-regulation of two candidate genes (P450 and NsLTP) in abas1 may contribute to the variation of ABA sensitivity. Our findings provide more valuable material and information toward the understanding of the mechanism of ABA response in barley.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"507 - 516"},"PeriodicalIF":3.2,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43162796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Appropriate timing of flowering is pivotal for tobacco, while chilling stress occurring at the seedling stage often undesirably leads to early flowering. However, the potential mechanism underlying chilling-induced early flowering remains unknown. Here, transcriptome sequencing was performed in tobacco with or without chilling at both seedling and budding stages. Chilling affected the expression of numerous genes at the seedling stage, while these dramatic expression changes were largely eliminated at the budding stage. A small number of genes related to metabolism, flower development, and stress tolerance continued to keep their altered expression patterns from the seedling stage to the budding stage. Many potential flowering-related genes involved in flowering pathways were identified and over half of them were differentially expressed. Functional analysis revealed that the down-regulation of NbXTH22 rendered tobacco less sensitive to chilling-induced early flowering. These results provide valuable resources for the investigation of flowering regulatory mechanisms and contribute to the genetic improvement of crops.
{"title":"Transcriptomic insights into the regulatory networks of chilling-induced early flower in tobacco (Nicotiana tabacum L.)","authors":"Guoyun Xu, Wuxia Guo, Zunqiang Li, Chen Wang, Yalong Xu, Jingjing Jin, Huina Zhou, Shulin Deng","doi":"10.1080/17429145.2022.2055175","DOIUrl":"https://doi.org/10.1080/17429145.2022.2055175","url":null,"abstract":"ABSTRACT Appropriate timing of flowering is pivotal for tobacco, while chilling stress occurring at the seedling stage often undesirably leads to early flowering. However, the potential mechanism underlying chilling-induced early flowering remains unknown. Here, transcriptome sequencing was performed in tobacco with or without chilling at both seedling and budding stages. Chilling affected the expression of numerous genes at the seedling stage, while these dramatic expression changes were largely eliminated at the budding stage. A small number of genes related to metabolism, flower development, and stress tolerance continued to keep their altered expression patterns from the seedling stage to the budding stage. Many potential flowering-related genes involved in flowering pathways were identified and over half of them were differentially expressed. Functional analysis revealed that the down-regulation of NbXTH22 rendered tobacco less sensitive to chilling-induced early flowering. These results provide valuable resources for the investigation of flowering regulatory mechanisms and contribute to the genetic improvement of crops.","PeriodicalId":16830,"journal":{"name":"Journal of Plant Interactions","volume":"17 1","pages":"496 - 506"},"PeriodicalIF":3.2,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44364354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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