Pub Date : 2024-08-08DOI: 10.29169/1927-5951.2024.14.02
N. Lonigro, E. Martello, F. Perondi, M. Bigliati, Z. R. Mohammed, A. Costale, E. Rosso, N. Bruni
Gastritis in pets necessitates effective acid suppression for successful treatment. However, the synergistic potential of antacid salts within natural feed products remains underexplored. In this in vitro study, we aimed to compare six supplements comprising natural ingredients for their ability to safeguard gastric cells and mitigate cytotoxicity under hyperacidity conditions. While Product 1 showed ineffectiveness in cell protection, Products 2, 3, 4, and 5 exhibited varying degrees of reversal of hyperacidity-induced cytotoxicity. Notably, Product 6 demonstrated superior efficacy in shielding gastric cells from acidic pH-induced cytotoxicity, displaying a dose-dependent response. These findings highlight the potential of natural supplements, particularly Product 6, as promising candidates for mitigating gastritis-related conditions in pets. Further research, including in vivo studies, is warranted to validate these observations and explore their clinical applicability.
{"title":"Assessing the Efficacy of Natural Pet Products in Protecting Gastric Cells and Reducing Cytotoxicity under Hyperacidity Conditions: An In Vitro Study","authors":"N. Lonigro, E. Martello, F. Perondi, M. Bigliati, Z. R. Mohammed, A. Costale, E. Rosso, N. Bruni","doi":"10.29169/1927-5951.2024.14.02","DOIUrl":"https://doi.org/10.29169/1927-5951.2024.14.02","url":null,"abstract":"\u0000Gastritis in pets necessitates effective acid suppression for successful treatment. However, the synergistic potential of antacid salts within natural feed products remains underexplored. In this in vitro study, we aimed to compare six supplements comprising natural ingredients for their ability to safeguard gastric cells and mitigate cytotoxicity under hyperacidity conditions. While Product 1 showed ineffectiveness in cell protection, Products 2, 3, 4, and 5 exhibited varying degrees of reversal of hyperacidity-induced cytotoxicity. Notably, Product 6 demonstrated superior efficacy in shielding gastric cells from acidic pH-induced cytotoxicity, displaying a dose-dependent response. These findings highlight the potential of natural supplements, particularly Product 6, as promising candidates for mitigating gastritis-related conditions in pets. Further research, including in vivo studies, is warranted to validate these observations and explore their clinical applicability.\u0000","PeriodicalId":16959,"journal":{"name":"Journal of Pharmacy and Nutrition Sciences","volume":"118 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141926335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.29169/1927-5951.2024.14.01
N. Lonigro, Z. R. Mohammed, F. Perondi, E. Martello, A. Costale, E. Rosso, N. Bruni
Oxidative stress plays a key role in the pathogenesis of liver diseases and can be involved in the inflammatory process of liver cells. The aim of this vitro study is to assess the antioxidant efficacy of three distinct components (fermented S-Acetyl -L-glutathione, Silybum marianum (L.) Gaern. and vitamin E all-rac-alpha-tocopheryl acetate) both individually and in combination (Glutasyl product). In addition, we also evaluated the combined antioxidant effect of the three ingredients on human hepatic cells subjected to oxidative stress induced by H2O2 treatment. The results showed the dose-dependent antioxidant potential of the three components suggesting promising applications in medical contexts. The synergistic antioxidant effects observed for the Glutasyl was more than the individual components. Additionally, Glutasyl showed not-toxic activity on human hepatocytes and it could be considered useful in mitigating cytotoxicity induced by oxidative stress.
氧化应激在肝脏疾病的发病机制中起着关键作用，并可能参与肝细胞的炎症过程。这项体外研究的目的是评估三种不同成分（发酵 S-乙酰基-L-谷胱甘肽、水飞蓟（L.）和维生素 E 全-rac-α-生育酚乙酸酯）单独或组合（Glutasyl 产品）的抗氧化功效。此外，我们还评估了三种成分在 H2O2 处理诱导的氧化应激作用下对人肝细胞的联合抗氧化作用。与单个成分相比，Glutasyl 的协同抗氧化效果更好。此外，Glutasyl 对人类肝细胞无毒性，可用于减轻氧化应激引起的细胞毒性。
{"title":"Antioxidant Effect of a Combination of S-Acetyl-L-Glutathione, Vitamin E, Silybum Marianumon Hepatic Cells under Oxidative Stress: An In Vitro Study","authors":"N. Lonigro, Z. R. Mohammed, F. Perondi, E. Martello, A. Costale, E. Rosso, N. Bruni","doi":"10.29169/1927-5951.2024.14.01","DOIUrl":"https://doi.org/10.29169/1927-5951.2024.14.01","url":null,"abstract":"Oxidative stress plays a key role in the pathogenesis of liver diseases and can be involved in the inflammatory process of liver cells. The aim of this vitro study is to assess the antioxidant efficacy of three distinct components (fermented S-Acetyl -L-glutathione, Silybum marianum (L.) Gaern. and vitamin E all-rac-alpha-tocopheryl acetate) both individually and in combination (Glutasyl product). In addition, we also evaluated the combined antioxidant effect of the three ingredients on human hepatic cells subjected to oxidative stress induced by H2O2 treatment.\u0000The results showed the dose-dependent antioxidant potential of the three components suggesting promising applications in medical contexts. The synergistic antioxidant effects observed for the Glutasyl was more than the individual components. Additionally, Glutasyl showed not-toxic activity on human hepatocytes and it could be considered useful in mitigating cytotoxicity induced by oxidative stress.","PeriodicalId":16959,"journal":{"name":"Journal of Pharmacy and Nutrition Sciences","volume":"6 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140381419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.29169/1927-5951.2023.13.06
Amusan A. Timothy, Emokpae A. Mathias
This study evaluated the protective effects of curcuminoids against cadmium-induced testicular injury in Albino rats. Male albino rats were divided into nine groups, group 1 (control), groups 2-4 received 120 mg/kg, 240 mg/kg, and 360 mg/kg curcuminoids daily for 28 days without testicular injury. Group 5 received 20 mg/kg cadmium chloride (CdCl2) solution every other day for 28 days (positive control), while group 6 received 20mg/kg CdCl2 + 240 mg/kg curcuminoids every other day for 28 days. Group 7 received 20mg/kg CdCl2 every other day for 28 days, then treated with 240 mg/kg for 14 days. Group 8 received 20 mg/kg CdCl2 solution every other day for 28 days, then treated with 240 mg curcuminoids for 28 days. Group 9 received 20 mg/kg CdCl2 solution every other day for 28 days and left to recover 28 days. Serum and seminal plasma malondialdehyde, catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase were assayed using Spectrophotometry technique. Semen analysis was determined microscopically. Sperm motility and count were significantly reduced, acrosome defect and percent abnormal sperm morphology were increased among the positive control group when compared with negative control group (p<0.05). Serum GPX, GSH, and SOD, semen GPx , GSH and SOD were significantly reduced (p<0.001), while MDA was significantly increased (p<0.001) in CdCl2 administered rats than negative control. The supplementation of Curcumins resulted in the improvement of sperm quality indices in a dose-dependent manner. Curcumin supplementation may significantly reverse the adverse effects of cadmium chloride testicular injury.
{"title":"Protective Effect of Curcuminoids Consumption on Cadmium-Induced Testicular Injury in Albino Rats","authors":"Amusan A. Timothy, Emokpae A. Mathias","doi":"10.29169/1927-5951.2023.13.06","DOIUrl":"https://doi.org/10.29169/1927-5951.2023.13.06","url":null,"abstract":"This study evaluated the protective effects of curcuminoids against cadmium-induced testicular injury in Albino rats. Male albino rats were divided into nine groups, group 1 (control), groups 2-4 received 120 mg/kg, 240 mg/kg, and 360 mg/kg curcuminoids daily for 28 days without testicular injury. Group 5 received 20 mg/kg cadmium chloride (CdCl2) solution every other day for 28 days (positive control), while group 6 received 20mg/kg CdCl2 + 240 mg/kg curcuminoids every other day for 28 days. Group 7 received 20mg/kg CdCl2 every other day for 28 days, then treated with 240 mg/kg for 14 days. Group 8 received 20 mg/kg CdCl2 solution every other day for 28 days, then treated with 240 mg curcuminoids for 28 days. Group 9 received 20 mg/kg CdCl2 solution every other day for 28 days and left to recover 28 days. Serum and seminal plasma malondialdehyde, catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase were assayed using Spectrophotometry technique. Semen analysis was determined microscopically. Sperm motility and count were significantly reduced, acrosome defect and percent abnormal sperm morphology were increased among the positive control group when compared with negative control group (p<0.05). Serum GPX, GSH, and SOD, semen GPx , GSH and SOD were significantly reduced (p<0.001), while MDA was significantly increased (p<0.001) in CdCl2 administered rats than negative control. The supplementation of Curcumins resulted in the improvement of sperm quality indices in a dose-dependent manner. Curcumin supplementation may significantly reverse the adverse effects of cadmium chloride testicular injury.","PeriodicalId":16959,"journal":{"name":"Journal of Pharmacy and Nutrition Sciences","volume":"17 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139452734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Haemorrhoidal disease (HD) is a very diffuse anorectal condition that involves a large part of the population, both male and female of every age. Among the procedures proposed to treat HD, conventional excisional surgery remains one of the most performed. Milligan-Morgan (MM) technique is one of the most used haemorrhoidectomy techniques. In this technique, the wounds are left open and re-epithelialization requires almost 3-5 weeks, in which patients generally experience pain and intense discomfort improving over the weeks.
Methods: The aim of this study was to evaluate the effect of topic administration of Benebeo Gel®, mainly composed by bergamot-derived flavonoids and hyaluronic acid, on post-operative wound healing after open MM haemorrhoidectomy. An observational prospective study was carried out, involving 205 patients aged between 18 and 75.
Results and Conclusion: The results after 2 weeks of treatment seem to be promising with a very good clinical outcome and patient satisfaction within 1 month.
{"title":"Clinical Trial: Management of Post-Haemorrhoidectomy Wound Healing by Bergamot Flavonoid-Based Gel and Sodium Hyaluronate: An Observational, Multicentric Trial","authors":"Danilo Cafaro, Luciano Onofrio, Alessandro Sturiale, Salvatore Puglia, Maria Stefania Sinicropi, Alessia Catalano, Gabriele Naldini","doi":"10.29169/1927-5951.2023.13.05","DOIUrl":"https://doi.org/10.29169/1927-5951.2023.13.05","url":null,"abstract":"Objective: Haemorrhoidal disease (HD) is a very diffuse anorectal condition that involves a large part of the population, both male and female of every age. Among the procedures proposed to treat HD, conventional excisional surgery remains one of the most performed. Milligan-Morgan (MM) technique is one of the most used haemorrhoidectomy techniques. In this technique, the wounds are left open and re-epithelialization requires almost 3-5 weeks, in which patients generally experience pain and intense discomfort improving over the weeks.
 Methods: The aim of this study was to evaluate the effect of topic administration of Benebeo Gel®, mainly composed by bergamot-derived flavonoids and hyaluronic acid, on post-operative wound healing after open MM haemorrhoidectomy. An observational prospective study was carried out, involving 205 patients aged between 18 and 75.
 Results and Conclusion: The results after 2 weeks of treatment seem to be promising with a very good clinical outcome and patient satisfaction within 1 month.","PeriodicalId":16959,"journal":{"name":"Journal of Pharmacy and Nutrition Sciences","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135857073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-30DOI: 10.29169/1927-5951.2023.13.04
N. Bruni, E. Martello, Valeria Gabriele, Francesca Perondi
Gastritis is a common finding in pets and acid suppression plays an important role in its management. Acid suppression is the most important component in the treatment of gastritis and prevention of its complications. There are many drugs commonly used in veterinary medicine but the use of natural feed supplements is increasing. The aim of this in vitro study is the comparison of four supplements based on a combination of antacid salts and natural products at two pH conditions (pH 2 and 4.5) and at five time points. In addition, we wanted to confirm the formation of the gel during the test. All the products demonstrated acid suppression activity. In particular, product A showed the best performance. All the products except one (product B) formed gel after five minutes from the beginning of the experiment confirming their protective activity. Based on this preliminary results, the product A resulted to be the most promising antacid and potentially gastric protective product compared to others.
{"title":"Acid Suppressant Activity of Feed Dietary Supplements for Dogs","authors":"N. Bruni, E. Martello, Valeria Gabriele, Francesca Perondi","doi":"10.29169/1927-5951.2023.13.04","DOIUrl":"https://doi.org/10.29169/1927-5951.2023.13.04","url":null,"abstract":"\u0000Gastritis is a common finding in pets and acid suppression plays an important role in its management. Acid suppression is the most important component in the treatment of gastritis and prevention of its complications. There are many drugs commonly used in veterinary medicine but the use of natural feed supplements is increasing. The aim of this in vitro study is the comparison of four supplements based on a combination of antacid salts and natural products at two pH conditions (pH 2 and 4.5) and at five time points. In addition, we wanted to confirm the formation of the gel during the test. All the products demonstrated acid suppression activity. In particular, product A showed the best performance. All the products except one (product B) formed gel after five minutes from the beginning of the experiment confirming their protective activity. Based on this preliminary results, the product A resulted to be the most promising antacid and potentially gastric protective product compared to others.\u0000","PeriodicalId":16959,"journal":{"name":"Journal of Pharmacy and Nutrition Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76885708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-18DOI: 10.29169/1927-5951.2023.13.03
R. Laurenti, Martina Fioretti, Luis Severino Martin Martin, Francesca Tanzini, D. Marotto, E. Gubinelli, A. Migliore
Osteoporosis is considered the most frequent rheumatic pathology in the elderly population. It is often accompanied by sarcopenia, defined as the progressive loss of skeletal muscle mass and strength. Together, these conditions increase the risk of falls, fractures, disability, lower quality of life, and frequent hospitalization. This means increased healthcare costs for all European countries, due to the increase in the average age and elderly population. Multiple pharmacological therapies are available for osteoporosis, but the prevention seems to be an important tool to reduce the risk of fracture and hospitalization of patients. A healthy lifestyle, consisting of the right amount of nutrients and micronutrients and physical exercise can help prevent the development of osteoporosis and sarcopenia in the elderly. This review brings together the information present in the literature on the benefits that nutrients and micronutrients, naturally present in foods, have on the prevention of these pathologies. it is suggested that a correct intake of proteins, calcium, phosphorus, magnesium, vitamin.D, vitamin C and antioxidants, vitamin K and omega 3, associated with constant physical exercise, can help to contain osteoporosis. It also indicates the right physical exercise for older osteoporotic adults. This document drawn up by the CReI-Food study group of the Italian College of Rheumatologists aims to provide, based on the most recent scientific evidence, indications on correct nutrition and lifestyle for the prevention and treatment of osteoporosis in older people.
{"title":"Clinical considerations on nutrition and lifestyle in old people with osteoporosis and sarcopenia by the CReI-Food study group of the Italian College of Rheumatologists","authors":"R. Laurenti, Martina Fioretti, Luis Severino Martin Martin, Francesca Tanzini, D. Marotto, E. Gubinelli, A. Migliore","doi":"10.29169/1927-5951.2023.13.03","DOIUrl":"https://doi.org/10.29169/1927-5951.2023.13.03","url":null,"abstract":"Osteoporosis is considered the most frequent rheumatic pathology in the elderly population. It is often accompanied by sarcopenia, defined as the progressive loss of skeletal muscle mass and strength. Together, these conditions increase the risk of falls, fractures, disability, lower quality of life, and frequent hospitalization. This means increased healthcare costs for all European countries, due to the increase in the average age and elderly population. Multiple pharmacological therapies are available for osteoporosis, but the prevention seems to be an important tool to reduce the risk of fracture and hospitalization of patients. A healthy lifestyle, consisting of the right amount of nutrients and micronutrients and physical exercise can help prevent the development of osteoporosis and sarcopenia in the elderly. This review brings together the information present in the literature on the benefits that nutrients and micronutrients, naturally present in foods, have on the prevention of these pathologies. it is suggested that a correct intake of proteins, calcium, phosphorus, magnesium, vitamin.D, vitamin C and antioxidants, vitamin K and omega 3, associated with constant physical exercise, can help to contain osteoporosis. It also indicates the right physical exercise for older osteoporotic adults. This document drawn up by the CReI-Food study group of the Italian College of Rheumatologists aims to provide, based on the most recent scientific evidence, indications on correct nutrition and lifestyle for the prevention and treatment of osteoporosis in older people.","PeriodicalId":16959,"journal":{"name":"Journal of Pharmacy and Nutrition Sciences","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86985890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-28DOI: 10.29169/1927-5951.2023.13.02
N. Obasi, C. Aloke, S. E. Obasi, A. Famurewa, S. Elom, P. M. Aja, L. O. Ajala
The nutrients and chemical contents of Persea americana, Dacryodes edulis, and Canarium scheinfurthi fruits and partial characterization of their seed oils were carried out to ascertain their nutritional benefits. The fruit pulps were analyzed for chemical (proximate) composition, amino acids profile, vitamins, and phytochemical and anti-nutritional compositions. Oils were extracted from the fruit seeds and the physico-chemical properties of the seed oils were determined according to the standard protocols. The results showed that the fruit pulps contained an abundance of macro- and micro-nutrients which varied significantly (p < 0.05) among the varieties with low anti-nutrients. The essential amino acid contents were high and varied significantly (p < 0.05) among the varieties. Glutamic acid, followed by aspartic acid had the highest concentration of the amino acids, while the concentrations of methionine and cysteine were low in all the varieties. The results also revealed high essential amino acids score values, above 100% for isoleucine and total aromatic amino acids. The physicochemical properties of the fruit oils showed that the oils were edible (low acid value) and may have industrial potential due to their low peroxide, iodine, and saponification values. Overall, the results showed that the pears are nutritionally rich and could serve dietetic and industrial purposes.
{"title":"Nutritional Assessment of Pulps and Partial Characterization of Seed Oils from Varieties of Pear Fruits","authors":"N. Obasi, C. Aloke, S. E. Obasi, A. Famurewa, S. Elom, P. M. Aja, L. O. Ajala","doi":"10.29169/1927-5951.2023.13.02","DOIUrl":"https://doi.org/10.29169/1927-5951.2023.13.02","url":null,"abstract":"\u0000The nutrients and chemical contents of Persea americana, Dacryodes edulis, and Canarium scheinfurthi fruits and partial characterization of their seed oils were carried out to ascertain their nutritional benefits. The fruit pulps were analyzed for chemical (proximate) composition, amino acids profile, vitamins, and phytochemical and anti-nutritional compositions. Oils were extracted from the fruit seeds and the physico-chemical properties of the seed oils were determined according to the standard protocols. The results showed that the fruit pulps contained an abundance of macro- and micro-nutrients which varied significantly (p < 0.05) among the varieties with low anti-nutrients. The essential amino acid contents were high and varied significantly (p < 0.05) among the varieties. Glutamic acid, followed by aspartic acid had the highest concentration of the amino acids, while the concentrations of methionine and cysteine were low in all the varieties. The results also revealed high essential amino acids score values, above 100% for isoleucine and total aromatic amino acids. The physicochemical properties of the fruit oils showed that the oils were edible (low acid value) and may have industrial potential due to their low peroxide, iodine, and saponification values. Overall, the results showed that the pears are nutritionally rich and could serve dietetic and industrial purposes.\u0000","PeriodicalId":16959,"journal":{"name":"Journal of Pharmacy and Nutrition Sciences","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89914749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-26DOI: 10.29169/1927-5951.2023.13.01
Suhair Albassit, Shaza Al laham, A. Al-Manadili
Drug-induced nephrotoxicity is a matter of global significance, with widespread implications for human health. Non-steroidal anti-inflammatory drugs (NSAIDs), such as Diclofenac Sodium, are widely used but have been associated with numerous adverse effects, including those on the gastrointestinal tract, liver, and kidney. Selenium, an essential element in mammalian biology, has demonstrated antioxidant properties that enable it to antagonize the effects of free radicals. The present study aimed to investigate the potential protective effect of Selenium against Diclofenac Sodium-induced nephrotoxicity in rats. The study sample consisted of 32 male albino Wistar rats and was randomly divided into 4 groups: Control group, Diclofenac group (Dic group) (administered at a dose of 50 mg/kg I.M., for 3 days), Selenium group (Se group) (administered at a dose of 1 mg/kg I.P., for 8 days), and Diclofenac plus Selenium group (Dic + Se group). Rats were sacrificed on the 9th day. The results were analyzed with a significance level of p<0.05. The findings revealed significant tubular and glomerular injuries in the Diclofenac group compared to the control group. However, there was no statistically significant improvement observed in the Dic + Se group. In conclusion, the results of this study suggest that Selenium affords partial protection against Diclofenac Sodium-induced nephrotoxicity, but it is not sufficient to provide complete protection.
{"title":"Histological Assessment of Selenium Protective Effect Against Diclofenac Sodium-Induced Nephrotoxicity in Rats","authors":"Suhair Albassit, Shaza Al laham, A. Al-Manadili","doi":"10.29169/1927-5951.2023.13.01","DOIUrl":"https://doi.org/10.29169/1927-5951.2023.13.01","url":null,"abstract":"Drug-induced nephrotoxicity is a matter of global significance, with widespread implications for human health. Non-steroidal anti-inflammatory drugs (NSAIDs), such as Diclofenac Sodium, are widely used but have been associated with numerous adverse effects, including those on the gastrointestinal tract, liver, and kidney. Selenium, an essential element in mammalian biology, has demonstrated antioxidant properties that enable it to antagonize the effects of free radicals. The present study aimed to investigate the potential protective effect of Selenium against Diclofenac Sodium-induced nephrotoxicity in rats. The study sample consisted of 32 male albino Wistar rats and was randomly divided into 4 groups: Control group, Diclofenac group (Dic group) (administered at a dose of 50 mg/kg I.M., for 3 days), Selenium group (Se group) (administered at a dose of 1 mg/kg I.P., for 8 days), and Diclofenac plus Selenium group (Dic + Se group). Rats were sacrificed on the 9th day. The results were analyzed with a significance level of p<0.05. The findings revealed significant tubular and glomerular injuries in the Diclofenac group compared to the control group. However, there was no statistically significant improvement observed in the Dic + Se group. In conclusion, the results of this study suggest that Selenium affords partial protection against Diclofenac Sodium-induced nephrotoxicity, but it is not sufficient to provide complete protection.","PeriodicalId":16959,"journal":{"name":"Journal of Pharmacy and Nutrition Sciences","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83668908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.29169/1927-5951.2022.12.11
M. Mileva, Adriana Dimitrova-Koleva, E. Tsvetanova, D. Krastev, A. Georgieva, A. Alexandrova, A. Galabov
This study aimed to estimate the protective effect of a combination of S-Adenosyl-L-methionine (SAM) as a precursor of glutathione and oseltamivir as a specific inhibitor of virus replication on oxidative damages caused by influenza infection in the liver of infected mice. Albino mice were inoculated with 10×LD50 of influenza virus A/Aichi/2/68(H3N2). Oseltamivir was applied for five days after infection, twice per day, in a dose of 2.5 mg/kg. SAM was applied for ten days in a 100 mg/kg dose, starting five days before inoculation. Markers of oxidative stress, mortality rate, mean survival time, index, and protection coefficient were followed. Influenza infection causes severe oxidative damage to the liver. All combinations of SAM and oseltamivir restored the levels of the biochemical markers to those in healthy animals and improved the virological parameters. A combination of SAM 100 mg/kg and oseltamivir 2.5 mg/kg, which is 1/4 of the optimal therapeutic mice dose, exhibits protection index and affects most effectively all the tested parameters. This study provides an easy-to-apply approach with a good therapeutic potential for co-medicating influenza infection with a specific antiviral agent and an antioxidant precursor.
{"title":"Mitigation of the Oxidative Damage in Liver Caused by Influenza Virus Infection in Mice by an Effective Combination of Oseltamivir and S-adenosyl-L-methionine (SAM)","authors":"M. Mileva, Adriana Dimitrova-Koleva, E. Tsvetanova, D. Krastev, A. Georgieva, A. Alexandrova, A. Galabov","doi":"10.29169/1927-5951.2022.12.11","DOIUrl":"https://doi.org/10.29169/1927-5951.2022.12.11","url":null,"abstract":"\u0000This study aimed to estimate the protective effect of a combination of S-Adenosyl-L-methionine (SAM) as a precursor of glutathione and oseltamivir as a specific inhibitor of virus replication on oxidative damages caused by influenza infection in the liver of infected mice. Albino mice were inoculated with 10×LD50 of influenza virus A/Aichi/2/68(H3N2). Oseltamivir was applied for five days after infection, twice per day, in a dose of 2.5 mg/kg. SAM was applied for ten days in a 100 mg/kg dose, starting five days before inoculation. Markers of oxidative stress, mortality rate, mean survival time, index, and protection coefficient were followed. Influenza infection causes severe oxidative damage to the liver. All combinations of SAM and oseltamivir restored the levels of the biochemical markers to those in healthy animals and improved the virological parameters. A combination of SAM 100 mg/kg and oseltamivir 2.5 mg/kg, which is 1/4 of the optimal therapeutic mice dose, exhibits protection index and affects most effectively all the tested parameters. This study provides an easy-to-apply approach with a good therapeutic potential for co-medicating influenza infection with a specific antiviral agent and an antioxidant precursor.\u0000","PeriodicalId":16959,"journal":{"name":"Journal of Pharmacy and Nutrition Sciences","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75194704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-27DOI: 10.29169/1927-5951.2022.12.13
S. Tripathi, N. Mishra, N. Mishra
The emergence of antimicrobial resistance and the side effects of synthetic drugs have raised an interest in searching for new antimicrobial compounds. The present study aims to evaluate in vitro antibacterial activity of green coffee and its active compounds (chlorogenic acid extract and caffeine extract) against some periodontogenic and nosocomial bacteria. The bioactive compounds, viz. chlorogenic acid and caffeine, were extracted through soxhlet extraction using methanol and water, respectively, and HPLC UV quantified these compounds. The study reported 3 CQA, 4 CQA, and 5 CQA as the significant chlorogenic acids in green coffee beans. Aqueous extract of green coffee beans (AGCB), which is dominant in caffeine, has been found to be the least effective against both periodontal and nosocomial bacteria. The result of our study revealed that the methanol extract of green coffee bean (MGCB), rich in chlorogenic acid, exhibits the highest inhibitory activity against periodontogenic bacteria, followed by the ethanol extract of green coffee bean (EGCB) and AGCB extract. EGCB extract was significantly effective against Staphylococcus epidermidis among selected nosocomial pathogens. AGCB extract was least effective against all bacteria. The results highlight that green coffee polyphenols, especially chlorogenic acid, could be used as antimicrobial agents in different biotechnological applications. The antibacterial property of green coffee highlights its potential as a naturally active antibacterial compound.
{"title":"Antibacterial Activity of Bioactive Compounds of Green Coffee Beans on Periodontogenic and Nosocomial Bacteria","authors":"S. Tripathi, N. Mishra, N. Mishra","doi":"10.29169/1927-5951.2022.12.13","DOIUrl":"https://doi.org/10.29169/1927-5951.2022.12.13","url":null,"abstract":"The emergence of antimicrobial resistance and the side effects of synthetic drugs have raised an interest in searching for new antimicrobial compounds. The present study aims to evaluate in vitro antibacterial activity of green coffee and its active compounds (chlorogenic acid extract and caffeine extract) against some periodontogenic and nosocomial bacteria. The bioactive compounds, viz. chlorogenic acid and caffeine, were extracted through soxhlet extraction using methanol and water, respectively, and HPLC UV quantified these compounds. The study reported 3 CQA, 4 CQA, and 5 CQA as the significant chlorogenic acids in green coffee beans. Aqueous extract of green coffee beans (AGCB), which is dominant in caffeine, has been found to be the least effective against both periodontal and nosocomial bacteria. The result of our study revealed that the methanol extract of green coffee bean (MGCB), rich in chlorogenic acid, exhibits the highest inhibitory activity against periodontogenic bacteria, followed by the ethanol extract of green coffee bean (EGCB) and AGCB extract. EGCB extract was significantly effective against Staphylococcus epidermidis among selected nosocomial pathogens. AGCB extract was least effective against all bacteria. The results highlight that green coffee polyphenols, especially chlorogenic acid, could be used as antimicrobial agents in different biotechnological applications. The antibacterial property of green coffee highlights its potential as a naturally active antibacterial compound.","PeriodicalId":16959,"journal":{"name":"Journal of Pharmacy and Nutrition Sciences","volume":"19 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82899271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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