Uropathogenic Escherichia coli (UPEC) is prevalent in urinary tract infections (UTIs). UPEC’s biofilm production enables it to invade and persist in the uroepithelium, leading to recurrent UTIs. The biofilm formation is associated with antibiotic resistance. To overcome this resistance, non-conventional compounds must be developed as an alternative to conventional antibiotics. Silver nanoparticles (AgNPs) are significant due to their antibacterial activity against diverse organisms. This study was done to investigate the antibacterial and anti-biofilm effects of AgNPs on UPEC. AgNPs were biosynthesized using Pseudomonas aeruginosa ATCC 27853. AgNPs were characterized using visual inspection and scanning electron microscopy. The Agar well diffusion method was employed to assess the antibacterial activity of AgNPs against UPEC isolates. The study utilized the tissue culture plate method to investigate both the biofilm and anti-biofilm properties of AgNPs. Following incubation, Ps.aeruginosa and silver nitrate (AgNO3) mixture exhibited a colour change from pale yellow to dark brown. The mean size of spherical AgNPs observed under a scanning electron microscope was 24.187 ± 8.019 nm. 130 UPECs were obtained. AgNPs exhibited antibacterial activity at a concentration of 20 µg/ml against all tested UPEC strains. Among UPEC strains that produced biofilms, a significant inhibition of 99.89 ± 0.45% was observed at a higher concentration of 512 µg/ml of AgNPs. Ps.aeruginosa produces nitrate reductase enzyme that can potentially convert AgNO3 to AgNPs. The biosynthesized AgNPs exhibit antibacterial and anti-biofilm activity against all tested UPEC strains.
{"title":"The Efficacy of Silver Nanoparticles in Combating Biofilm Formation by Uropathogenic Escherichia coli","authors":"Pradnya Atmaram Jadhav, Shubhangi Aniruddha Gadgil","doi":"10.22207/jpam.17.4.37","DOIUrl":"https://doi.org/10.22207/jpam.17.4.37","url":null,"abstract":"Uropathogenic Escherichia coli (UPEC) is prevalent in urinary tract infections (UTIs). UPEC’s biofilm production enables it to invade and persist in the uroepithelium, leading to recurrent UTIs. The biofilm formation is associated with antibiotic resistance. To overcome this resistance, non-conventional compounds must be developed as an alternative to conventional antibiotics. Silver nanoparticles (AgNPs) are significant due to their antibacterial activity against diverse organisms. This study was done to investigate the antibacterial and anti-biofilm effects of AgNPs on UPEC. AgNPs were biosynthesized using Pseudomonas aeruginosa ATCC 27853. AgNPs were characterized using visual inspection and scanning electron microscopy. The Agar well diffusion method was employed to assess the antibacterial activity of AgNPs against UPEC isolates. The study utilized the tissue culture plate method to investigate both the biofilm and anti-biofilm properties of AgNPs. Following incubation, Ps.aeruginosa and silver nitrate (AgNO3) mixture exhibited a colour change from pale yellow to dark brown. The mean size of spherical AgNPs observed under a scanning electron microscope was 24.187 ± 8.019 nm. 130 UPECs were obtained. AgNPs exhibited antibacterial activity at a concentration of 20 µg/ml against all tested UPEC strains. Among UPEC strains that produced biofilms, a significant inhibition of 99.89 ± 0.45% was observed at a higher concentration of 512 µg/ml of AgNPs. Ps.aeruginosa produces nitrate reductase enzyme that can potentially convert AgNO3 to AgNPs. The biosynthesized AgNPs exhibit antibacterial and anti-biofilm activity against all tested UPEC strains.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"44 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139211089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammed R. Mohaisen, S. Lafi, Sawasan Q.T. Al-Quhli
Microbes show a high antimicrobial resistance due to a high rate of mutations predisposed by many factors, especially the abuse of antibiotics. Therefore, there is a great need for an alternative therapeutic agent for infectious diseases caused by microbes resistant to antibiotics. Bacteriophages are viruses parasitizing microbes, that got a big scientist’s attention due to their ability as an alternative therapy for severe bacterial infections. This study is devoted to identifying bacteriophage from river water on tested pathogenic isolates isolated from clinical cases of UTI in vitro and finding out the effect of phage on these bacterial isolates as an initial step of further in vivo phage therapeutic study on the same tested isolates. The results showed a significant bactericidal effect of the isolated bacteriophages against the pathogenic bacterial isolates.
{"title":"Antibacterial Activity of River Water Bacteriophage against Multidrug-resistant Gram-negative Bacteria, An In vitro Study","authors":"Mohammed R. Mohaisen, S. Lafi, Sawasan Q.T. Al-Quhli","doi":"10.22207/jpam.17.4.30","DOIUrl":"https://doi.org/10.22207/jpam.17.4.30","url":null,"abstract":"Microbes show a high antimicrobial resistance due to a high rate of mutations predisposed by many factors, especially the abuse of antibiotics. Therefore, there is a great need for an alternative therapeutic agent for infectious diseases caused by microbes resistant to antibiotics. Bacteriophages are viruses parasitizing microbes, that got a big scientist’s attention due to their ability as an alternative therapy for severe bacterial infections. This study is devoted to identifying bacteriophage from river water on tested pathogenic isolates isolated from clinical cases of UTI in vitro and finding out the effect of phage on these bacterial isolates as an initial step of further in vivo phage therapeutic study on the same tested isolates. The results showed a significant bactericidal effect of the isolated bacteriophages against the pathogenic bacterial isolates.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"22 10","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139254233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. A. Johnnie, R. Issac, M. L. Prabha, Levin Anbu Gomez
This study focused on the isolation of laccase enzyme from the fungus Pleurotus ostreatus and its application in the biodegradation of various pollutants present in textile industrial effluent, including chemicals, ions, salts, heavy metals, brittle metals, total suspended solids, total dissolved solids, chemical oxygen demand, biological oxygen demand, minerals, total hardness, total alkalinity, turbidity, electrical conductivity, and dyes. Textile industrial effluent poses a significant threat to the environment, contaminating water bodies and posing risks to human, animal, and plant life. This study employed an economical and ecofriendly biological approach for wastewater treatment, distinguishing it from traditional physical and chemical methods. The optimum temperature of laccase is found to be 30 degree Celsius and pH is 3. Enzyme activity of laccase is 7.25 U/ml. This fugal laccase decolorizes textile Industrial dye effluent containing various dyes, such as Turquoise VG, Black B, Yellow R, Methyl red, Trypan blue, and Acid Orange 7. Laccase depicts maximum decolorization efficacy on Black B dye. Similarly, Laccase from P.ostreatus shows higher decolorization efficacy when compared to other fungal laccase. Additionally, the study assessed the biodegradation of various wastewater quality parameters, including physical and chemical parameters like toxic heavy metals and ions. This research of isolation, characterization, and utilizing laccase from P. ostreatus for the bioremediation of textile industrial effluent wastewater containing dyes, toxic chemicals, ions and metals is effective, economical and ecofriendly.
{"title":"Biodegradation Assay of Heavy Metals and Dyes Decolorization in Textile Industrial Effluent using Laccase Isolated from Pleurotus ostreatus","authors":"D. A. Johnnie, R. Issac, M. L. Prabha, Levin Anbu Gomez","doi":"10.22207/jpam.17.4.29","DOIUrl":"https://doi.org/10.22207/jpam.17.4.29","url":null,"abstract":"This study focused on the isolation of laccase enzyme from the fungus Pleurotus ostreatus and its application in the biodegradation of various pollutants present in textile industrial effluent, including chemicals, ions, salts, heavy metals, brittle metals, total suspended solids, total dissolved solids, chemical oxygen demand, biological oxygen demand, minerals, total hardness, total alkalinity, turbidity, electrical conductivity, and dyes. Textile industrial effluent poses a significant threat to the environment, contaminating water bodies and posing risks to human, animal, and plant life. This study employed an economical and ecofriendly biological approach for wastewater treatment, distinguishing it from traditional physical and chemical methods. The optimum temperature of laccase is found to be 30 degree Celsius and pH is 3. Enzyme activity of laccase is 7.25 U/ml. This fugal laccase decolorizes textile Industrial dye effluent containing various dyes, such as Turquoise VG, Black B, Yellow R, Methyl red, Trypan blue, and Acid Orange 7. Laccase depicts maximum decolorization efficacy on Black B dye. Similarly, Laccase from P.ostreatus shows higher decolorization efficacy when compared to other fungal laccase. Additionally, the study assessed the biodegradation of various wastewater quality parameters, including physical and chemical parameters like toxic heavy metals and ions. This research of isolation, characterization, and utilizing laccase from P. ostreatus for the bioremediation of textile industrial effluent wastewater containing dyes, toxic chemicals, ions and metals is effective, economical and ecofriendly.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"36 12","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A thermophilic bacterial strain having the ability to produce L-tryptophan enzymatically was isolated and identified from a less explored hot spring of West Bengal. The isolate was identified using polyphasic taxonomic approach as a strain of Bacillus licheniformis. Initially, the 16S rRNA gene and later the whole genome of the isolate was sequenced and submitted to the NCBI Gene Bank for future reference. The isolate showed considerable tryptophan synthase activity and may be a potential candidate for mass production of L-tryptophan by enzymatic means.
{"title":"Enzymatic Production of L-tryptophan by a Thermophilic Strain of Bacillus licheniformis Isolated from a Local Hot Spring of Paniphala, Asansol Area of West Bengal","authors":"Anindita Roy","doi":"10.22207/jpam.17.4.27","DOIUrl":"https://doi.org/10.22207/jpam.17.4.27","url":null,"abstract":"A thermophilic bacterial strain having the ability to produce L-tryptophan enzymatically was isolated and identified from a less explored hot spring of West Bengal. The isolate was identified using polyphasic taxonomic approach as a strain of Bacillus licheniformis. Initially, the 16S rRNA gene and later the whole genome of the isolate was sequenced and submitted to the NCBI Gene Bank for future reference. The isolate showed considerable tryptophan synthase activity and may be a potential candidate for mass production of L-tryptophan by enzymatic means.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"36 8","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139255023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As sugarcane molasses is converted into ethanol, a sizable volume of effluent with high biochemical oxygen demand (BOD) and chemical oxygen demand(COD) is generated. This effluent contains melanoidin. Melanodin is a chemical broken down by certain bacteria and can produce peroxidases, phenoxidases, laccases, and mono- and dioxygenases. The primary function of these bacteria is to break down complex hydrocarbons, including aromatics such as coloring pigments. This study aimed to identify melanoidin-decolorizing microorganisms in natural resources that are thermally resistant and have the potential to be used in industrial-scale distillery treatment for effluent applications. A total of 55 distinct isolates were tested on a solid medium, including molasses pigments. Three thermotolerant bacterial isolates were identified as melanoidin-decolorizing agents: Bacillus nitratireducens (B2), Bacillus paramycoides (B1), and Brucellatritici (B3). These isolates under went additional optimization for decolorization under various physicochemical and nutritional conditions. At 40°C, B. nitratireducens (B2) exhibited the highest degree of decolorization (86%) among the three species while using 0.5% glucose(w/v), 0.5% peptone(w/v), 0.05% MgSO4, and 0.01% KH2PO4 at a pH of 6.0 over 40 h of incubation under static conditions. In submerged fermentation, the B2 strain of B.nitratireducens can withstand higher temperatures and requires only a small amount of carbon (0.5%, [w/v]) and nitrogen sources (0.5%, [w/v]). Therefore, it is feasible to use melanoidin on an industrial scale to decolorize distilled effluents.
{"title":"Decolorization of Distillery Effluent by the Novel Bacterial Strain Bacillus nitratireducens (B2)","authors":"Ajad Patel, Ranjan Singh, R. Gaur","doi":"10.22207/jpam.17.4.26","DOIUrl":"https://doi.org/10.22207/jpam.17.4.26","url":null,"abstract":"As sugarcane molasses is converted into ethanol, a sizable volume of effluent with high biochemical oxygen demand (BOD) and chemical oxygen demand(COD) is generated. This effluent contains melanoidin. Melanodin is a chemical broken down by certain bacteria and can produce peroxidases, phenoxidases, laccases, and mono- and dioxygenases. The primary function of these bacteria is to break down complex hydrocarbons, including aromatics such as coloring pigments. This study aimed to identify melanoidin-decolorizing microorganisms in natural resources that are thermally resistant and have the potential to be used in industrial-scale distillery treatment for effluent applications. A total of 55 distinct isolates were tested on a solid medium, including molasses pigments. Three thermotolerant bacterial isolates were identified as melanoidin-decolorizing agents: Bacillus nitratireducens (B2), Bacillus paramycoides (B1), and Brucellatritici (B3). These isolates under went additional optimization for decolorization under various physicochemical and nutritional conditions. At 40°C, B. nitratireducens (B2) exhibited the highest degree of decolorization (86%) among the three species while using 0.5% glucose(w/v), 0.5% peptone(w/v), 0.05% MgSO4, and 0.01% KH2PO4 at a pH of 6.0 over 40 h of incubation under static conditions. In submerged fermentation, the B2 strain of B.nitratireducens can withstand higher temperatures and requires only a small amount of carbon (0.5%, [w/v]) and nitrogen sources (0.5%, [w/v]). Therefore, it is feasible to use melanoidin on an industrial scale to decolorize distilled effluents.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"12 2","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139255685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SARS-CoV-2 is highly contagious, which spreads even by patients having no clinical symptoms or also from people suffering with only mild symptoms. The gold standard test for its diagnosis is reverse-transcription PCR (RT-PCR) but at times of pandemic, Rapid antigen tests (RAT) are required, which has a very less turn-around time. Evaluation of the performance of COVID-19 Rapid antigen test in comparison to SARS-CoV-2 RT-PCR using nasopharyngeal swab, in relation to RNA dependent RNA polymerase (RdRp) Cycle threshold (Ct) values. This observational and cross-sectional study was done on patients coming with features of Influenza-like illness (ILI) or for any aerosol generating procedure or on high-risk patients seeking hospitalization. Both RT-PCR and RAT for COVID-19 were done on samples collected from each patient and results were compared. Altogether, 5314 samples were tested, out of which 104 (01.95 %) & 229 (04.31 %) samples were found positive by the RAT & RT PCR test, respectively. Sensitivity, specificity, PPV and NPV of RAT were found to be 44.54%, 99.96%, 98.08% and 97.56%, respectively. 98.9 % of samples with Ct value ≤ 20 were positive by RAT, whereas only 2.2% samples having Ct value ≥ 26 were found to be positive. Cases having lower Ct values were found to be more symptomatic and vice-versa. RAT are not efficient in detecting the virus in samples showing high Ct values (Ct ≥ 26) by RT-PCR test. Patients with samples showing low Ct values (Ct ≤ 20) had more severe symptoms and vice-versa.
{"title":"Performance Evaluation of Rapid Antigen Test for COVID-19 with SARS-CoV-2 RT-PCR using Nasopharyngeal Swab with Special Reference to RNA Dependent RNA Polymerase (RdRp) Cycle Threshold Values","authors":"Vidyut Prakash, Kumar Saurabh, Rishikesh Kumar, Randhir Kumar, N. Kumari, Shailesh Kumar","doi":"10.22207/jpam.17.4.28","DOIUrl":"https://doi.org/10.22207/jpam.17.4.28","url":null,"abstract":"SARS-CoV-2 is highly contagious, which spreads even by patients having no clinical symptoms or also from people suffering with only mild symptoms. The gold standard test for its diagnosis is reverse-transcription PCR (RT-PCR) but at times of pandemic, Rapid antigen tests (RAT) are required, which has a very less turn-around time. Evaluation of the performance of COVID-19 Rapid antigen test in comparison to SARS-CoV-2 RT-PCR using nasopharyngeal swab, in relation to RNA dependent RNA polymerase (RdRp) Cycle threshold (Ct) values. This observational and cross-sectional study was done on patients coming with features of Influenza-like illness (ILI) or for any aerosol generating procedure or on high-risk patients seeking hospitalization. Both RT-PCR and RAT for COVID-19 were done on samples collected from each patient and results were compared. Altogether, 5314 samples were tested, out of which 104 (01.95 %) & 229 (04.31 %) samples were found positive by the RAT & RT PCR test, respectively. Sensitivity, specificity, PPV and NPV of RAT were found to be 44.54%, 99.96%, 98.08% and 97.56%, respectively. 98.9 % of samples with Ct value ≤ 20 were positive by RAT, whereas only 2.2% samples having Ct value ≥ 26 were found to be positive. Cases having lower Ct values were found to be more symptomatic and vice-versa. RAT are not efficient in detecting the virus in samples showing high Ct values (Ct ≥ 26) by RT-PCR test. Patients with samples showing low Ct values (Ct ≤ 20) had more severe symptoms and vice-versa.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"47 3","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139256771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Chaudhury, T. Biswas, Koushik Bose, Prabir Sengupta, Arghya Nath, Nivedita Mukherjee, A. Basu, SK Mukhopadhyay
Cervical cancer is a notable cause of mortality and morbidity among women of reproductive age. Human papillomavirus (HPV) is the leading cause of cervical cancer among women. Among 170 types of HPV; HPV-16 and -18 are responsible for cervical cancer. The overexpression of oncoproteins E6 and E7 are predominantly responsible for causing neoplasia. The presence of koilocytosis/koilocytotic atypia is the diagnostic point of HPV infection in pap smears. To identify the circulating types of HPV and determine the various risk factors associated with HPV infection, 100 vaginal biopsies or swabs were taken from patients suspected with cervical cancer, and qualitative and semi-quantitative real-time PCR were performed. PCR primers (GP5+/GP6+) based on a conserved region of the HPV-L1open reading frame(ORF) gene were used for the detection of HPV strains, while another set of primers was used for detecting the E6 gene (HPV-16) and E7 gene (HPV-18). The results showed an HPV infection rate of 23%. Furthermore, the prevalent genotype was found to be HPV-16 (73.91%), followed by HPV-18 (26.1%), while mixed infections of both HPV-16 and -18 accounted for 21.74%. In addition, an age of above 45 years, multiple pregnancies, low socioeconomic status, postmenopausal state, anemia, and early coitarche were significantly associated with HPV infection. These results provide the basis for the formulation of an appropriate strategy for disease monitoring to determine the frequency and distribution pattern of HPV infection.
{"title":"Prevalence and Risk Assessment of Human Papillomavirus Infection in a Bengali Cohort","authors":"N. Chaudhury, T. Biswas, Koushik Bose, Prabir Sengupta, Arghya Nath, Nivedita Mukherjee, A. Basu, SK Mukhopadhyay","doi":"10.22207/jpam.17.4.25","DOIUrl":"https://doi.org/10.22207/jpam.17.4.25","url":null,"abstract":"Cervical cancer is a notable cause of mortality and morbidity among women of reproductive age. Human papillomavirus (HPV) is the leading cause of cervical cancer among women. Among 170 types of HPV; HPV-16 and -18 are responsible for cervical cancer. The overexpression of oncoproteins E6 and E7 are predominantly responsible for causing neoplasia. The presence of koilocytosis/koilocytotic atypia is the diagnostic point of HPV infection in pap smears. To identify the circulating types of HPV and determine the various risk factors associated with HPV infection, 100 vaginal biopsies or swabs were taken from patients suspected with cervical cancer, and qualitative and semi-quantitative real-time PCR were performed. PCR primers (GP5+/GP6+) based on a conserved region of the HPV-L1open reading frame(ORF) gene were used for the detection of HPV strains, while another set of primers was used for detecting the E6 gene (HPV-16) and E7 gene (HPV-18). The results showed an HPV infection rate of 23%. Furthermore, the prevalent genotype was found to be HPV-16 (73.91%), followed by HPV-18 (26.1%), while mixed infections of both HPV-16 and -18 accounted for 21.74%. In addition, an age of above 45 years, multiple pregnancies, low socioeconomic status, postmenopausal state, anemia, and early coitarche were significantly associated with HPV infection. These results provide the basis for the formulation of an appropriate strategy for disease monitoring to determine the frequency and distribution pattern of HPV infection.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"14 10","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139258659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amal F. Makled, Sahar A.M. Ali, Ahmed B. Mahmoud, Marwa E. Eltoukhy, Reem M. Elkholy, Athar F. Lasheen, Asmaa Mohammed Elbrolosy
Global dissemination of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Gram-negative bacteria (GNB) such as carbapenemase-producing Enterobacterales has resulted in reviving colistin as a final therapeutic alternative. Colistin resistance foretold a catastrophe. We aimed to detect the rates of carbapenems and colistin resistance among hospital-acquired Enterobacterales species, verify the underlying mechanisms and provide antibiogram for colistin-resistant isolates. The collected Enterobacterales isolates were tested for their antimicrobial susceptibility by the disk diffusion method and agar dilution was utilized for both imipenem and colistin. The production of ESβLs and carbapenemases was phenotypically assessed by the combined disk (CDT) and modified carbapenem inactivation (mCIM) tests, respectively. Possible attributes for colistin resistance were explored by detection of both plasmid- and efflux pump-mediated mechanisms. By multiplex PCR assay, carbapenem resistance (blaNDM-1 & blaOXA-48) and mobilized colistin-resistant-1 (mcr-1) genes were identified. A total of 160 Enterobacterales isolates were obtained of which 68.8% were MDR, 25% were XDR and 6.3% were pandrug-resistant (PDR) isolates with no statistically significant difference among Enterobacterales species (P> 0.05). Carbapenems resistance was detected in 41.3% (66/160) while colistin resistance was detected in 22% (36/160) of isolates. Proteus mirabilis expressed the highest rate of colistin resistance (100%; 16/16), followed by Enterobacter aerogenes (23.1%; 6/26), E. coli (13%; 6/46) and K.pneumoniae (11.1%; 8/72). One hundred percent (36/36) of colistin-resistant isolates proved efflux pump activity for colistin. However; only 2% (2/100) of tested Enterobacterales carried mcr-1 gene through molecular analysis. Colistin-resistant isolates exhibited variable susceptibility to the tested antimicrobial agents of which fosfomycin was the highest (94.1%). Efflux pump activity played a major role for colistin resistance among Enterobacterales species and fosfomycin could be a promising therapeutic option.
{"title":"Colistin Resistance among Enterobacterales Isolates: Underlying Mechanisms and Alternative Treatment Options","authors":"Amal F. Makled, Sahar A.M. Ali, Ahmed B. Mahmoud, Marwa E. Eltoukhy, Reem M. Elkholy, Athar F. Lasheen, Asmaa Mohammed Elbrolosy","doi":"10.22207/jpam.17.4.24","DOIUrl":"https://doi.org/10.22207/jpam.17.4.24","url":null,"abstract":"Global dissemination of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Gram-negative bacteria (GNB) such as carbapenemase-producing Enterobacterales has resulted in reviving colistin as a final therapeutic alternative. Colistin resistance foretold a catastrophe. We aimed to detect the rates of carbapenems and colistin resistance among hospital-acquired Enterobacterales species, verify the underlying mechanisms and provide antibiogram for colistin-resistant isolates. The collected Enterobacterales isolates were tested for their antimicrobial susceptibility by the disk diffusion method and agar dilution was utilized for both imipenem and colistin. The production of ESβLs and carbapenemases was phenotypically assessed by the combined disk (CDT) and modified carbapenem inactivation (mCIM) tests, respectively. Possible attributes for colistin resistance were explored by detection of both plasmid- and efflux pump-mediated mechanisms. By multiplex PCR assay, carbapenem resistance (blaNDM-1 & blaOXA-48) and mobilized colistin-resistant-1 (mcr-1) genes were identified. A total of 160 Enterobacterales isolates were obtained of which 68.8% were MDR, 25% were XDR and 6.3% were pandrug-resistant (PDR) isolates with no statistically significant difference among Enterobacterales species (P> 0.05). Carbapenems resistance was detected in 41.3% (66/160) while colistin resistance was detected in 22% (36/160) of isolates. Proteus mirabilis expressed the highest rate of colistin resistance (100%; 16/16), followed by Enterobacter aerogenes (23.1%; 6/26), E. coli (13%; 6/46) and K.pneumoniae (11.1%; 8/72). One hundred percent (36/36) of colistin-resistant isolates proved efflux pump activity for colistin. However; only 2% (2/100) of tested Enterobacterales carried mcr-1 gene through molecular analysis. Colistin-resistant isolates exhibited variable susceptibility to the tested antimicrobial agents of which fosfomycin was the highest (94.1%). Efflux pump activity played a major role for colistin resistance among Enterobacterales species and fosfomycin could be a promising therapeutic option.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"25 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135042663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ashfaq Ahmad Shah, Shivangi Singh, Shubhika Saini, Aarushi Pundir, Kushal Saxena, Amit Gupta
This study aims to scrutinize the phenolic secondary metabolites in the polar peel decocture of Malus domestica var Maharaji via hyphenated techniques along with the study of the antibacterial, anti-candida, and tyrosinase inhibitory potential of bioactive compound-rich fractions. Preliminary phenolics go over was performed together with thin layer chromatography before the polar decocture was subjected to hyphenated techniques. FTIR investigation revealed the C-O bonds as in phenols, O-H bond stretch, and vibrations of alcohols and carboxylic acids as well as portrayed the C-H and >C=O stretches among other functional groups all of which are representative of phenolic and polyphenolic compounds. GC-MS perusal demonstrated the presence of bioactive compounds like Quercetin (13.04%), Ascorbic acid (6.48%), p-Coumaric acid (6.17%), Caffeic acid (5.69 %), Mallic acid (5.44%), Apigenin (5.28%), Citric acid (5.15%), Gallic acid (4.38%), Cyanidin (3.52%), and Ferulic acid (3.51%). Kirby-Bauer method followed by the resazurin microtiter assay technique (REMA) for MIC/MBC against six MTCC bacterial strains and one yeast, all producing stubborn opportunistic infection in humans, was used to assess the antibacterial property of all the bioactive rich fractions. Some fractions comparatively revealed a good activity index (AI) against tested microbes. MIC concentrations for bacteria ranged from 15-24 mg/ml while a lower MBC value recorded was 18 mg/ml. Methanol fraction revealed significant tyrosinase inhibitory activity by revealing IC50 of 980.98 µg/ml when L-Tyrosine was substrate and IC50 of 830.68 µg/ml when L-DOPA was substrate when juxtaposed to standard kojic acid that revealed IC50 of 128.822 µg/ml when L-Tyrosine was substrate and IC50 of 149.43 µg/ml when L-DOPA was substrate. The bioactive compounds possessed by the fractions, may be synergistically, turned out to be more effective in the diphenolase reaction and kojic acid acts more effectively in the monophenolase one. It was inferred that peel phenolics of this malus variety have a lot of therapeutic potential in the context of bacterial infections and pigmentation disorders.
{"title":"A Perusal of Phenolics Within Polar Peel Fractions of Malus domestica var Maharaji via Hyphenated Techniques Cum Anti-microbial with REMA and Tyrosinase Inhibitory Potential thereof","authors":"Ashfaq Ahmad Shah, Shivangi Singh, Shubhika Saini, Aarushi Pundir, Kushal Saxena, Amit Gupta","doi":"10.22207/jpam.17.4.19","DOIUrl":"https://doi.org/10.22207/jpam.17.4.19","url":null,"abstract":"This study aims to scrutinize the phenolic secondary metabolites in the polar peel decocture of Malus domestica var Maharaji via hyphenated techniques along with the study of the antibacterial, anti-candida, and tyrosinase inhibitory potential of bioactive compound-rich fractions. Preliminary phenolics go over was performed together with thin layer chromatography before the polar decocture was subjected to hyphenated techniques. FTIR investigation revealed the C-O bonds as in phenols, O-H bond stretch, and vibrations of alcohols and carboxylic acids as well as portrayed the C-H and >C=O stretches among other functional groups all of which are representative of phenolic and polyphenolic compounds. GC-MS perusal demonstrated the presence of bioactive compounds like Quercetin (13.04%), Ascorbic acid (6.48%), p-Coumaric acid (6.17%), Caffeic acid (5.69 %), Mallic acid (5.44%), Apigenin (5.28%), Citric acid (5.15%), Gallic acid (4.38%), Cyanidin (3.52%), and Ferulic acid (3.51%). Kirby-Bauer method followed by the resazurin microtiter assay technique (REMA) for MIC/MBC against six MTCC bacterial strains and one yeast, all producing stubborn opportunistic infection in humans, was used to assess the antibacterial property of all the bioactive rich fractions. Some fractions comparatively revealed a good activity index (AI) against tested microbes. MIC concentrations for bacteria ranged from 15-24 mg/ml while a lower MBC value recorded was 18 mg/ml. Methanol fraction revealed significant tyrosinase inhibitory activity by revealing IC50 of 980.98 µg/ml when L-Tyrosine was substrate and IC50 of 830.68 µg/ml when L-DOPA was substrate when juxtaposed to standard kojic acid that revealed IC50 of 128.822 µg/ml when L-Tyrosine was substrate and IC50 of 149.43 µg/ml when L-DOPA was substrate. The bioactive compounds possessed by the fractions, may be synergistically, turned out to be more effective in the diphenolase reaction and kojic acid acts more effectively in the monophenolase one. It was inferred that peel phenolics of this malus variety have a lot of therapeutic potential in the context of bacterial infections and pigmentation disorders.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135141925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gaurav Verma, Subham Ravi Nayak, Swetapadma Jena, Subhra Snigdha Panda, Dipti Pattnaik, A.K. Praharaj, Nipa Singh
In recent years, a wide range of clinical infections are being caused by carbapenem-resistant Enterobacterales, Acinetobacter baumannii and Pseudomonas aeruginosa. This is a matter of great concern, as carbapenem-resistant infections have fewer treatment options. The Enterobacterales comprises a large group of bacterial species commonly causing infections in healthcare settings. The most common bacteria are Escherichia coli and Klebsiella pneumoniae, which can cause both nosocomial and community-acquired infections. This study aimed to determine the prevalence of carbapenem-resistant Enterobacterales, P. aeruginosa, and A. baumannii, in a tertiary care center in India. The study was conducted over a period of seven months, from May 2022 to November 2022. The specimens were processed at the Microbiology Laboratory of Kalinga Institute of Medical Sciences- Pradyumna Bal Memorial Hospital, Bhubaneswar. Standard procedures were used to process the clinical specimens brought to the laboratory. Carbapenem-resistant isolates were screened according to the CLSI 2022 guidelines. This study included 3,006 isolates of Enterobacterales, A. baumannii, and P. aeruginosa. Of these, 29.40% (n = 844) were found to be carbapenem resistant. The breakup is as follows: 689 (77.94%) were Enterobacterales, 108 (12.21%) were A. baumannii, and 87 (9.84%) were P. aeruginosa. Thus, our investigation revealed an overall prevalence of carbapenem-resistant Enterobacterales, A. baumannii, and P. aeruginosa of 29.40%, which corresponds to previous studies in India. Early patient screening, isolation, and contact prevention measures will help reduce infection transmission. Further, larger multi-centric studies are required to obtain a wider perspective regarding this issue.
{"title":"Prevalence of Carbapenem-Resistant Enterobacterales, Acinetobacter baumannii, and Pseudomonas aeruginosa in a Tertiary Care Hospital in Eastern India: A Pilot Study","authors":"Gaurav Verma, Subham Ravi Nayak, Swetapadma Jena, Subhra Snigdha Panda, Dipti Pattnaik, A.K. Praharaj, Nipa Singh","doi":"10.22207/jpam.17.4.21","DOIUrl":"https://doi.org/10.22207/jpam.17.4.21","url":null,"abstract":"In recent years, a wide range of clinical infections are being caused by carbapenem-resistant Enterobacterales, Acinetobacter baumannii and Pseudomonas aeruginosa. This is a matter of great concern, as carbapenem-resistant infections have fewer treatment options. The Enterobacterales comprises a large group of bacterial species commonly causing infections in healthcare settings. The most common bacteria are Escherichia coli and Klebsiella pneumoniae, which can cause both nosocomial and community-acquired infections. This study aimed to determine the prevalence of carbapenem-resistant Enterobacterales, P. aeruginosa, and A. baumannii, in a tertiary care center in India. The study was conducted over a period of seven months, from May 2022 to November 2022. The specimens were processed at the Microbiology Laboratory of Kalinga Institute of Medical Sciences- Pradyumna Bal Memorial Hospital, Bhubaneswar. Standard procedures were used to process the clinical specimens brought to the laboratory. Carbapenem-resistant isolates were screened according to the CLSI 2022 guidelines. This study included 3,006 isolates of Enterobacterales, A. baumannii, and P. aeruginosa. Of these, 29.40% (n = 844) were found to be carbapenem resistant. The breakup is as follows: 689 (77.94%) were Enterobacterales, 108 (12.21%) were A. baumannii, and 87 (9.84%) were P. aeruginosa. Thus, our investigation revealed an overall prevalence of carbapenem-resistant Enterobacterales, A. baumannii, and P. aeruginosa of 29.40%, which corresponds to previous studies in India. Early patient screening, isolation, and contact prevention measures will help reduce infection transmission. Further, larger multi-centric studies are required to obtain a wider perspective regarding this issue.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135141789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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