Pub Date : 2025-02-03DOI: 10.1016/j.supflu.2024.106508
Maria N.H. Cupaja , Camila Guindani , Frederico W. Tavares , Papa M. Ndiaye
Propane is considered an alternative in enzymatic polyester synthesis due to its favorable enzymatic interactions and mild operating pressures. In this study, enzymatic polymerizations were carried out by ring-opening polymerization of the monomer globalide at 65 °C, using pressurized propane as solvent. Mass ratios of propane:globalide of 1:2, 1:1, and 2:1 were evaluated, as well as pressure conditions of 30, 70, 110, and 150 bar. The enzyme concentration was kept at 5 % relative to globalide mass, with a fixed reaction time of two hours. Results showed monomer conversions up to 93 % and molecular weights ranging from 17.5 kDa to 125.9 kDa, surpassing those with pressurized CO2. Working pressure had an effect in monomer conversion, suggesting that both the increase in propane viscosity as well as the confinement of the system (compressibility) play important roles in the reaction, but depending on the working pressure, one of the effects will be dominant.
{"title":"Effect of pressure and monomer concentration on ring-opening enzymatic polymerization of globalide in pressurized propane","authors":"Maria N.H. Cupaja , Camila Guindani , Frederico W. Tavares , Papa M. Ndiaye","doi":"10.1016/j.supflu.2024.106508","DOIUrl":"10.1016/j.supflu.2024.106508","url":null,"abstract":"<div><div>Propane is considered an alternative in enzymatic polyester synthesis due to its favorable enzymatic interactions and mild operating pressures. In this study, enzymatic polymerizations were carried out by ring-opening polymerization of the monomer globalide at 65 °C, using pressurized propane as solvent. Mass ratios of propane:globalide of 1:2, 1:1, and 2:1 were evaluated, as well as pressure conditions of 30, 70, 110, and 150 bar. The enzyme concentration was kept at 5 % relative to globalide mass, with a fixed reaction time of two hours. Results showed monomer conversions up to 93 % and molecular weights ranging from 17.5 kDa to 125.9 kDa, surpassing those with pressurized CO<sub>2</sub>. Working pressure had an effect in monomer conversion, suggesting that both the increase in propane viscosity as well as the confinement of the system (compressibility) play important roles in the reaction, but depending on the working pressure, one of the effects will be dominant.</div></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":"220 ","pages":"Article 106508"},"PeriodicalIF":3.4,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-02DOI: 10.1016/j.supflu.2025.106539
Mohammed A. Al-Hakami , Ahmed Farid Ibrahim , Khaled Z. Abdelgawad
Innovation and sustainability are critical for maximizing reserves in the petroleum industry, especially in deep heavy oil reservoirs where traditional thermal EOR techniques often underperform. CO2-flooding offers a promising alternative, with its effectiveness dependent on achieving minimum miscibility pressure (MMP). Reducing MMP offers higher recovery factors while avoiding issues due to formation fracturing at high injection pressures. This study evaluates ionic liquids (ILs), 1-methyl-3-octylimidazolium chloride ([MOIM]Cl) and 1-decyl-3-methylimidazolium chloride ([DMIM]Cl), for lowering MMP in CO2-crude oil systems. Results showed [MOIM]Cl and [DMIM]Cl reduced MMP by 17 % and 13.5 %, outperforming n-butanol and non-ionic surfactants, which achieved 6 % and 9 % reductions, respectively. For extra heavy oil, [MOIM]Cl reduced MMP by 15.5 %. The ILs also lowered first contact miscibility pressure across crude oils with asphaltene content ranging from 6.4 to 36 wt%. These findings highlight ILs' potential to improve CO2 miscibility in heavy oil reservoirs, providing a sustainable solution for enhanced oil recovery.
{"title":"Applications of ionic liquids in improving CO2 miscibility in crude oil for enhanced oil recovery and CO2 sequestration applications","authors":"Mohammed A. Al-Hakami , Ahmed Farid Ibrahim , Khaled Z. Abdelgawad","doi":"10.1016/j.supflu.2025.106539","DOIUrl":"10.1016/j.supflu.2025.106539","url":null,"abstract":"<div><div>Innovation and sustainability are critical for maximizing reserves in the petroleum industry, especially in deep heavy oil reservoirs where traditional thermal EOR techniques often underperform. CO<sub>2</sub>-flooding offers a promising alternative, with its effectiveness dependent on achieving minimum miscibility pressure (MMP). Reducing MMP offers higher recovery factors while avoiding issues due to formation fracturing at high injection pressures. This study evaluates ionic liquids (ILs), 1-methyl-3-octylimidazolium chloride ([MOIM]Cl) and 1-decyl-3-methylimidazolium chloride ([DMIM]Cl), for lowering MMP in CO<sub>2</sub>-crude oil systems. Results showed [MOIM]Cl and [DMIM]Cl reduced MMP by 17 % and 13.5 %, outperforming n-butanol and non-ionic surfactants, which achieved 6 % and 9 % reductions, respectively. For extra heavy oil, [MOIM]Cl reduced MMP by 15.5 %. The ILs also lowered first contact miscibility pressure across crude oils with asphaltene content ranging from 6.4 to 36 wt%. These findings highlight ILs' potential to improve CO<sub>2</sub> miscibility in heavy oil reservoirs, providing a sustainable solution for enhanced oil recovery.</div></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":"219 ","pages":"Article 106539"},"PeriodicalIF":3.4,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-29DOI: 10.1016/j.supflu.2025.106538
Alican Ertas, Cerag Dilek
The solubility of a bifunctional polyhedral oligomeric silsesquioxane, trifluoropropylisobutyl (TFIB) POSS, in supercritical carbon dioxide (scCO2) was investigated. In the temperature and pressure ranges of 308 K to 323 K and 8.8 MPa to 14.5 MPa, respectively, TFIB POSS is soluble in scCO2 up to 2.0 × 10−2 by weight fraction corresponding to 1.0 × 10−3 by mole fraction, which is between the solubility of its monofunctional counterparts, octaisobutyl POSS and octatrifluoropropyl POSS. The study includes the modeling of the TFIB POSS-CO2 binary system phase equilibrium with the density-based semi-empirical equations and Peng Robinson+COSMO segment activity coefficient (PR+COSMOSAC) equation of state (EOS). The prediction of the equation of state has been improved for the cage-structured molecule by introducing new electrostatic and dispersion contributions. While the density-based relations provide better fits to the solubility isotherms, the main advantage of the EOS is its applicability for a priori phase equilibrium predictions for the CO2-POSS systems in the absence of the solute critical properties.
{"title":"High-pressure phase behavior of the carbon dioxide-trifluoropropylisobutyl polyhedral oligomeric silsesquioxane binary system","authors":"Alican Ertas, Cerag Dilek","doi":"10.1016/j.supflu.2025.106538","DOIUrl":"10.1016/j.supflu.2025.106538","url":null,"abstract":"<div><div>The solubility of a bifunctional polyhedral oligomeric silsesquioxane, trifluoropropylisobutyl (TFIB) POSS, in supercritical carbon dioxide (scCO<sub>2</sub>) was investigated. In the temperature and pressure ranges of 308 K to 323 K and 8.8 MPa to 14.5 MPa, respectively, TFIB POSS is soluble in scCO<sub>2</sub> up to 2.0 × 10<sup>−2</sup> by weight fraction corresponding to 1.0 × 10<sup>−3</sup> by mole fraction, which is between the solubility of its monofunctional counterparts, octaisobutyl POSS and octatrifluoropropyl POSS. The study includes the modeling of the TFIB POSS-CO<sub>2</sub> binary system phase equilibrium with the density-based semi-empirical equations and Peng Robinson+COSMO segment activity coefficient (PR+COSMOSAC) equation of state (EOS). The prediction of the equation of state has been improved for the cage-structured molecule by introducing new electrostatic and dispersion contributions. While the density-based relations provide better fits to the solubility isotherms, the main advantage of the EOS is its applicability for a priori phase equilibrium predictions for the CO<sub>2</sub>-POSS systems in the absence of the solute critical properties.</div></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":"219 ","pages":"Article 106538"},"PeriodicalIF":3.4,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143228559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-28DOI: 10.1016/j.supflu.2025.106537
Yan Ren , Le Zeng , Shaogeng Zhong , Weidong Wu , Yingying Yang , Qiguo Yang
To improve the performance of printed circuit heat exchanger (PCHE) in the supercritical carbon dioxide (SCO2) Brayton cycle system, the mathematic-physical model of the heat exchange unit was established, based on a new-type rectangular microchannel PCHE (RM-PCHE) manufacturing by total-etching technology in this study. And the influence mechanism and laws on the flow and heat transfer of the different fin distributions and shapes were numerically investigate. Results showed average simulation deviations of 0.9 % for outlet temperature and 13.3 % for heat transfer quantity, meeting accuracy requirement. Uniform fin distribution at 10 mm intervals significantly reduced pressure drop and improved comprehensive heat transfer performance by 14.8 %. When elliptical and airfoil fins were selected for the hot-side and cold-side channels, the flow characteristic significantly improved and the heat transfer process became more stable, improving the single-side performance by 15.9 % and 12.3 %, respectively.
{"title":"Optimization of fin for rectangular microchannel printed circuit heat exchanger using supercritical CO2 as working fluid","authors":"Yan Ren , Le Zeng , Shaogeng Zhong , Weidong Wu , Yingying Yang , Qiguo Yang","doi":"10.1016/j.supflu.2025.106537","DOIUrl":"10.1016/j.supflu.2025.106537","url":null,"abstract":"<div><div>To improve the performance of printed circuit heat exchanger (PCHE) in the supercritical carbon dioxide (SCO<sub>2</sub>) Brayton cycle system, the mathematic-physical model of the heat exchange unit was established, based on a new-type rectangular microchannel PCHE (RM-PCHE) manufacturing by total-etching technology in this study. And the influence mechanism and laws on the flow and heat transfer of the different fin distributions and shapes were numerically investigate. Results showed average simulation deviations of 0.9 % for outlet temperature and 13.3 % for heat transfer quantity, meeting accuracy requirement. Uniform fin distribution at 10 mm intervals significantly reduced pressure drop and improved comprehensive heat transfer performance by 14.8 %. When elliptical and airfoil fins were selected for the hot-side and cold-side channels, the flow characteristic significantly improved and the heat transfer process became more stable, improving the single-side performance by 15.9 % and 12.3 %, respectively.</div></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":"219 ","pages":"Article 106537"},"PeriodicalIF":3.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-21DOI: 10.1016/j.supflu.2025.106523
Xiao-Chang Lu , Biao-Qi Chen , Sheng-Qing Li , Jian-Fei Xu , Ranjith Kumar Kankala , Shi-Bin Wang , Ai-Zheng Chen
The poor water solubility of hesperetin (HST) has impeded its relevant medical applications. To address this problem, the supercritical anti-solvent (SAS) technique was used to prepare HST-polyvinylpyrrolidone (HST-PVP) sub-microparticles. Additionally, the Box-Behnken Design was used to optimize three parameters (temperature, total solute concentration, and pressure) to determine the optimal process conditions, which were determined to be 40 ℃, 5 mg/mL, and 100 bar. The physicochemical properties, drug release, and in vitro anti-cancer efficacy of the designed particles under the optimal conditions were systematically investigated. The drug loading of HST in HST-PVP sub-microparticles was quantified at 12.73 %. The dissolution rate of SAS-treated HST-PVP sub-microparticles was significantly enhanced compared to that of pure HST, leading to a higher anti-cancer efficiency of the HST-PVP sub-microparticles than that of pure HST. These findings indicate that the SAS technique holds significant potential for enhancing the bioavailability of hydrophobic drugs.
{"title":"Preparation of hesperetin-polyvinylpyrrolidone sub-microparticles by supercritical anti-solvent technique for improved anti-cancer efficiency","authors":"Xiao-Chang Lu , Biao-Qi Chen , Sheng-Qing Li , Jian-Fei Xu , Ranjith Kumar Kankala , Shi-Bin Wang , Ai-Zheng Chen","doi":"10.1016/j.supflu.2025.106523","DOIUrl":"10.1016/j.supflu.2025.106523","url":null,"abstract":"<div><div>The poor water solubility of hesperetin (HST) has impeded its relevant medical applications. To address this problem, the supercritical anti-solvent (SAS) technique was used to prepare HST-polyvinylpyrrolidone (HST-PVP) sub-microparticles. Additionally, the Box-Behnken Design was used to optimize three parameters (temperature, total solute concentration, and pressure) to determine the optimal process conditions, which were determined to be 40 ℃, 5 mg/mL, and 100 bar. The physicochemical properties, drug release, and <em>in vitro</em> anti-cancer efficacy of the designed particles under the optimal conditions were systematically investigated. The drug loading of HST in HST-PVP sub-microparticles was quantified at 12.73 %. The dissolution rate of SAS-treated HST-PVP sub-microparticles was significantly enhanced compared to that of pure HST, leading to a higher anti-cancer efficiency of the HST-PVP sub-microparticles than that of pure HST. These findings indicate that the SAS technique holds significant potential for enhancing the bioavailability of hydrophobic drugs.</div></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":"219 ","pages":"Article 106523"},"PeriodicalIF":3.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-17DOI: 10.1016/j.supflu.2025.106522
Ana Paula de Souza e Silva , Flávia Cristina Seabra Pires , Maria Caroline Rodrigues Ferreira , Maria Eduarda Ferraz de Carvalho , Letícia Maria Martins Siqueira , Fernando de Freitas Maués de Azevedo , Eduardo Gama Ortiz de Menezes , Raul Nunes Carvalho Junior
The Amazon bioeconomy demands technologies that transform raw materials into high-value bioproducts. This work presents the assembly of a supercritical extractor designed for the Amazon, aiming for efficiency and adaptation of the equipment to the local conditions such as humidity and temperature that provide a directly influence in the phase equilibrium in the CO2 cylinder. Therefore, a gas booster system was developed, eliminating the need for CO2 liquefaction. This innovation simplified operations, reduced costs, and increased energy efficiency. The unit, tested against Soxhlet and commercial SFE methods, achieved 72 % of extraction efficiency using jambu inflorescences, improving product quality. This equipment promotes the use of sustainable resources and supports the Amazon bioeconomy.
{"title":"A new supercritical extraction equipment in the Amazon: Supercritical extractor efficiency upgrade provided by gas booster performance","authors":"Ana Paula de Souza e Silva , Flávia Cristina Seabra Pires , Maria Caroline Rodrigues Ferreira , Maria Eduarda Ferraz de Carvalho , Letícia Maria Martins Siqueira , Fernando de Freitas Maués de Azevedo , Eduardo Gama Ortiz de Menezes , Raul Nunes Carvalho Junior","doi":"10.1016/j.supflu.2025.106522","DOIUrl":"10.1016/j.supflu.2025.106522","url":null,"abstract":"<div><div>The Amazon bioeconomy demands technologies that transform raw materials into high-value bioproducts. This work presents the assembly of a supercritical extractor designed for the Amazon, aiming for efficiency and adaptation of the equipment to the local conditions such as humidity and temperature that provide a directly influence in the phase equilibrium in the CO<sub>2</sub> cylinder. Therefore, a gas booster system was developed, eliminating the need for CO<sub>2</sub> liquefaction. This innovation simplified operations, reduced costs, and increased energy efficiency. The unit, tested against Soxhlet and commercial SFE methods, achieved 72 % of extraction efficiency using jambu inflorescences, improving product quality. This equipment promotes the use of sustainable resources and supports the Amazon bioeconomy.</div></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":"219 ","pages":"Article 106522"},"PeriodicalIF":3.4,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16DOI: 10.1016/j.supflu.2025.106520
Xuwen Wang , Zhifeng Liu , Haoda Ruan , Xinyu Li , Lei Zhang
The rapid advancement of the photovoltaic (PV) industry has elevated the recycling and reuse of decommissioned PV modules to critical challenges, essential for tackling environmental concerns and achieving resource reutilization. To facilitate the efficient layering of PV modules and recovery of valuable materials, this study proposes a PV module degradation and recycling process utilizing supercritical n-butanol. A systematic evaluation was conducted to assess the effects of various factors on the dissolution ratio of PV modules, with an appropriate range of process parameters selected for optimization. A second-order regression model was developed to predict the dissolution ratio of the EVA film. Comparison of actual dissolution ratio with model predictions confirmed the high accuracy of the fitted model. Finally, characterization and elemental analysis of cells recovered from the supercritical process demonstrated the effectiveness of this recycling method, achieving more thorough degradation on the cell surfaces.
{"title":"Employing supercritical n-butanol to remove the EVA film for the delamination of photovoltaic laminated module","authors":"Xuwen Wang , Zhifeng Liu , Haoda Ruan , Xinyu Li , Lei Zhang","doi":"10.1016/j.supflu.2025.106520","DOIUrl":"10.1016/j.supflu.2025.106520","url":null,"abstract":"<div><div>The rapid advancement of the photovoltaic (PV) industry has elevated the recycling and reuse of decommissioned PV modules to critical challenges, essential for tackling environmental concerns and achieving resource reutilization. To facilitate the efficient layering of PV modules and recovery of valuable materials, this study proposes a PV module degradation and recycling process utilizing supercritical n-butanol. A systematic evaluation was conducted to assess the effects of various factors on the dissolution ratio of PV modules, with an appropriate range of process parameters selected for optimization. A second-order regression model was developed to predict the dissolution ratio of the EVA film. Comparison of actual dissolution ratio with model predictions confirmed the high accuracy of the fitted model. Finally, characterization and elemental analysis of cells recovered from the supercritical process demonstrated the effectiveness of this recycling method, achieving more thorough degradation on the cell surfaces.</div></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":"219 ","pages":"Article 106520"},"PeriodicalIF":3.4,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143228558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16DOI: 10.1016/j.supflu.2025.106521
Yiheng Dou, Yoshito Oshima, Makoto Akizuki
The use of the CO2-expanded liquid method for enhancing the extraction performance of deep eutectic solvents (DES) was evaluated. The extraction of crocin I from gardenia fruits using a DES composed of choline chloride and propylene glycol (1:3) was enhanced by the formation of a CO2-expanded DES. Visualization showed a biphasic system in which the DES-rich phase, and not the CO2 phase, extracted crocin I. Temperature and pressure were the key parameters influencing the effect of CO2 addition, likely due to CO2 solubility in the DES. Kinetic studies using a second-order model showed that CO2 improved both the extraction rate and yield, particularly at higher pressures and lower temperatures. At 40 °C, CO2 addition doubled the extraction rate at 10 MPa and increased the yield by 8 % at 12 MPa.
{"title":"Effect of CO2 addition on crocin i extraction from gardenia fruits using CO2-expanded deep eutectic solvent","authors":"Yiheng Dou, Yoshito Oshima, Makoto Akizuki","doi":"10.1016/j.supflu.2025.106521","DOIUrl":"10.1016/j.supflu.2025.106521","url":null,"abstract":"<div><div>The use of the CO<sub>2</sub>-expanded liquid method for enhancing the extraction performance of deep eutectic solvents (DES) was evaluated. The extraction of crocin I from gardenia fruits using a DES composed of choline chloride and propylene glycol (1:3) was enhanced by the formation of a CO<sub>2</sub>-expanded DES. Visualization showed a biphasic system in which the DES-rich phase, and not the CO<sub>2</sub> phase, extracted crocin I. Temperature and pressure were the key parameters influencing the effect of CO<sub>2</sub> addition, likely due to CO<sub>2</sub> solubility in the DES. Kinetic studies using a second-order model showed that CO<sub>2</sub> improved both the extraction rate and yield, particularly at higher pressures and lower temperatures. At 40 °C, CO<sub>2</sub> addition doubled the extraction rate at 10 MPa and increased the yield by 8 % at 12 MPa.</div></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":"219 ","pages":"Article 106521"},"PeriodicalIF":3.4,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-15DOI: 10.1016/j.supflu.2025.106518
Gizem Zihna , Busra Kilic , Bengisu Topuz , Gulcin Gunal , Halil Murat Aydin
The increasing prevalence of meniscus injuries highlights the critical need for the development of effective repair strategies. Currently, there is ongoing exploration of materials possessing microscopic and macroscopic characteristics akin to authentic meniscus tissue. In this study, meniscus tissue was subjected to a comparative evaluation of two different decellularization techniques which one of these involved conventional decellularization technique; one novel approach involved the utilization of supercritical carbon dioxide (scCO₂) technology on meniscus tissue. All decellularized tissues underwent biochemical, histological, microscopic, mechanical and cytotoxic evaluations. The optimized method, combining physical pretreatment, enzymatic agitation with trypsin agent, and chemical agitation with SDS agent, achieved a remarkable reduction of 82 % in genomic DNA content. Physical pre-treatment in scCO₂ decellularization facilitated enhanced penetration depth with trypsin, resulting in optimal group demonstrating a 76 % reduction in DNA content. While histological examinations and biochemical analyses indicated no alteration in collagen quantities, the conventional decellularization group exhibited a 42 % decline in GAG content, whereas the scCO2 group showed a 58 % decrease. Compressive modulus decreased from 22.8 ± 0.91 MPa in decellularized tissues to 15.26 ± 0.28 MPa in conventionally decellularized scaffold and 14.49 ± 0.48 MPa in scCO2 decellularized tissue, attributed to GAG destruction. Cytotoxicity assessments of the examined tissues revealed cell viability levels exceeding 75 % in both groups. Both approaches demonstrate potential for producing high-quality biomaterials; nevertheless, further research endeavors may lead to enhancements in the extracellular matrix preservation, mechanical characteristics, and facilitation of biological responses using supercritical fluid-based methodologies.
{"title":"Comparative analysis of supercritical-based and chemical-based decellularization technique for meniscus tissue","authors":"Gizem Zihna , Busra Kilic , Bengisu Topuz , Gulcin Gunal , Halil Murat Aydin","doi":"10.1016/j.supflu.2025.106518","DOIUrl":"10.1016/j.supflu.2025.106518","url":null,"abstract":"<div><div>The increasing prevalence of meniscus injuries highlights the critical need for the development of effective repair strategies. Currently, there is ongoing exploration of materials possessing microscopic and macroscopic characteristics akin to authentic meniscus tissue. In this study, meniscus tissue was subjected to a comparative evaluation of two different decellularization techniques which one of these involved conventional decellularization technique; one novel approach involved the utilization of supercritical carbon dioxide (scCO₂) technology on meniscus tissue. All decellularized tissues underwent biochemical, histological, microscopic, mechanical and cytotoxic evaluations. The optimized method, combining physical pretreatment, enzymatic agitation with trypsin agent, and chemical agitation with SDS agent, achieved a remarkable reduction of 82 % in genomic DNA content. Physical pre-treatment in scCO₂ decellularization facilitated enhanced penetration depth with trypsin, resulting in optimal group demonstrating a 76 % reduction in DNA content. While histological examinations and biochemical analyses indicated no alteration in collagen quantities, the conventional decellularization group exhibited a 42 % decline in GAG content, whereas the scCO<sub>2</sub> group showed a 58 % decrease. Compressive modulus decreased from 22.8 ± 0.91 MPa in decellularized tissues to 15.26 ± 0.28 MPa in conventionally decellularized scaffold and 14.49 ± 0.48 MPa in scCO<sub>2</sub> decellularized tissue, attributed to GAG destruction. Cytotoxicity assessments of the examined tissues revealed cell viability levels exceeding 75 % in both groups. Both approaches demonstrate potential for producing high-quality biomaterials; nevertheless, further research endeavors may lead to enhancements in the extracellular matrix preservation, mechanical characteristics, and facilitation of biological responses using supercritical fluid-based methodologies.</div></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":"219 ","pages":"Article 106518"},"PeriodicalIF":3.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-13DOI: 10.1016/j.supflu.2025.106519
Hugo Andersson Dantas Medeiros , Jean-Patrick Bazile , Filipe Xavier Feitosa , Hosiberto Batista de Sant’Ana , Jean-Luc Daridon
This study investigates the high-pressure phase behavior of synthetic systems formed by the blending of a ternary heavy liquid mixture: 2,2,4,4,6,8,8 heptamethylnonane + dodecylcyclohexane + o-terphenyl – with light gas mixtures. Two different gas mixtures were examined: methane + ethane + propane and methane + ethane + propane + carbon dioxide. Five global compositions were studied for the first gas+liquid system and seven for the second, with temperatures ranging from T = 293.15 K to 373.15 K and pressures up to p = 70 MPa. One hundred and eighteen pressure transitions were measured, including fluid-fluid and solid-fluid transitions. The Peng-Robinson equation of state, with the fully predictive mixing rule PPR78, was used to predict the observed fluid-fluid transitions, showing qualitatively good agreement with experiments.
{"title":"Fluid phase behavior of asymmetric synthetic mixture + gas: Experimental and modeling studies","authors":"Hugo Andersson Dantas Medeiros , Jean-Patrick Bazile , Filipe Xavier Feitosa , Hosiberto Batista de Sant’Ana , Jean-Luc Daridon","doi":"10.1016/j.supflu.2025.106519","DOIUrl":"10.1016/j.supflu.2025.106519","url":null,"abstract":"<div><div>This study investigates the high-pressure phase behavior of synthetic systems formed by the blending of a ternary heavy liquid mixture: 2,2,4,4,6,8,8 heptamethylnonane + dodecylcyclohexane + <em>o</em>-terphenyl – with light gas mixtures. Two different gas mixtures were examined: methane + ethane + propane and methane + ethane + propane + carbon dioxide. Five global compositions were studied for the first gas+liquid system and seven for the second, with temperatures ranging from <em>T</em> = 293.15 K to 373.15 K and pressures up to <em>p</em> = 70 MPa. One hundred and eighteen pressure transitions were measured, including fluid-fluid and solid-fluid transitions. The Peng-Robinson equation of state, with the fully predictive mixing rule PPR78, was used to predict the observed fluid-fluid transitions, showing qualitatively good agreement with experiments.</div></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":"219 ","pages":"Article 106519"},"PeriodicalIF":3.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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