Pub Date : 2023-04-28DOI: 10.1590/2317-1545v45264166
S. E. V. Gomes, Raquel Araujo Gomes, B. F. Dantas
Abstract: Seed production, quality and germination are likely to be affected by a drastic climate change in semi-arid areas predicted for the end of the century. We evaluated Anadenanthera colubrina var. cebil (Griseb.) Altschu (Fabaceae) seeds of different sizes, populations and harvest years for germination and tolerance to environmental stresses aiming to predict impacts of future climate. Seeds were accessed for germination temperature, salinity and osmotic limits and requirements. Germination of large and small seeds harvested in different populations was evaluated in optimum and stressful temperature, salinity and water deficit. A glasshouse pot assay tested weekly irrigation regimes and seedlings emergence and growth. Optimal temperature for seeds germination was 34.8 oC and limits were 5.6 oC and 50.9 oC. Large and small-sized seeds do not differ in germination, however small seeds are more efficient in stressful conditions. Seedlings can emerge and grow under small weekly irrigation for four months. The predicted increase in temperature will not impair germination, however, the time available for seedling establishment will decrease due to lacking rainfall. The increase in the amount of small-sized seeds produced in drought years is a strategy for coping with harsh environments, rather than a decrease in seed quality.
摘要:预计本世纪末半干旱地区的种子生产、质量和萌发可能受到剧烈气候变化的影响。我们评估了Anadenanthera colubrina var. cebil (Griseb.)。以豆科植物为研究对象，对不同大小、不同种群、不同收获期的豆科植物种子的萌发和对环境胁迫的耐受性进行了研究，旨在预测未来气候的影响。获取种子的萌发温度、盐度和渗透极限和要求。在适宜温度、盐度和水分亏缺条件下，对不同种群收获的大、小种子的萌发进行了评价。一项温室盆栽试验测试了每周的灌溉制度和幼苗的出苗和生长情况。种子萌发最适温度为34.8℃，极限温度为5.6℃和50.9℃。大种子和小种子在发芽方面没有区别，但小种子在压力条件下更有效。幼苗可以在每周少量灌溉的情况下发芽生长，持续四个月。预测的温度升高不会影响种子的萌发，但由于降雨不足，可用于育苗的时间会减少。干旱年份小粒种子产量的增加是应对恶劣环境的一种策略，而不是种子质量的下降。
{"title":"Climate and seed size of a dry forest species: influence on seed production, physiological quality, and tolerance to abiotic stresses","authors":"S. E. V. Gomes, Raquel Araujo Gomes, B. F. Dantas","doi":"10.1590/2317-1545v45264166","DOIUrl":"https://doi.org/10.1590/2317-1545v45264166","url":null,"abstract":"Abstract: Seed production, quality and germination are likely to be affected by a drastic climate change in semi-arid areas predicted for the end of the century. We evaluated Anadenanthera colubrina var. cebil (Griseb.) Altschu (Fabaceae) seeds of different sizes, populations and harvest years for germination and tolerance to environmental stresses aiming to predict impacts of future climate. Seeds were accessed for germination temperature, salinity and osmotic limits and requirements. Germination of large and small seeds harvested in different populations was evaluated in optimum and stressful temperature, salinity and water deficit. A glasshouse pot assay tested weekly irrigation regimes and seedlings emergence and growth. Optimal temperature for seeds germination was 34.8 oC and limits were 5.6 oC and 50.9 oC. Large and small-sized seeds do not differ in germination, however small seeds are more efficient in stressful conditions. Seedlings can emerge and grow under small weekly irrigation for four months. The predicted increase in temperature will not impair germination, however, the time available for seedling establishment will decrease due to lacking rainfall. The increase in the amount of small-sized seeds produced in drought years is a strategy for coping with harsh environments, rather than a decrease in seed quality.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-28DOI: 10.1590/2317-1545v45268856
J. C. Medeiros, E. R. Carvalho, Dayliane Bernardes de Andrade, Luiz Fernando de Souza Moraes, J. M. E. Lima, Mônica Amurim Freitas Massa
Abstract: Seed treatment is important for protecting seed and seedling from pests and/or pathogens. Seed treatment can be performed generally in two ways: industrial seed treatment (IST), performed by seed companies, or on-farm treatment (OFT). These treatment technologies will affect the final quality of the process. Therefore, the objective of this study was to characterize the seed quality profile of ISTs and OFTs in Brazilian agribusiness in terms of functional quality and dose application effectiveness (DAE). Seed samples treated by both processes were collected in 6 producing states of Brazil, Mato Grosso, Goiás, Paraná, Minas Gerais, São Paulo, and Santa Catarina. To evaluate the functional quality of the treatments, the coating percentage was determined by high-resolution image analysis with GroundEye® equipment, and the insecticide active ingredient DAE amount was determined with high-performance liquid chromatography (HPLC). Image analysis proved to be a promising technique to evaluate seed coating, in addition, samples treated with “on farm” technology had greater variation and heterogeneity in coating and active ingredient dose, while samples treated industrially showed greater homogeneity, uniformity, effectiveness of applied products. There was a strong positive correlation between coating with phytosanitary products and DAE in treated corn seeds, important parameters in the evaluation of the quality of the treatment.
{"title":"Quality of corn seed industrial seed treatment (IST) and on-farm treatment (OFT) in Brazilian agribusiness","authors":"J. C. Medeiros, E. R. Carvalho, Dayliane Bernardes de Andrade, Luiz Fernando de Souza Moraes, J. M. E. Lima, Mônica Amurim Freitas Massa","doi":"10.1590/2317-1545v45268856","DOIUrl":"https://doi.org/10.1590/2317-1545v45268856","url":null,"abstract":"Abstract: Seed treatment is important for protecting seed and seedling from pests and/or pathogens. Seed treatment can be performed generally in two ways: industrial seed treatment (IST), performed by seed companies, or on-farm treatment (OFT). These treatment technologies will affect the final quality of the process. Therefore, the objective of this study was to characterize the seed quality profile of ISTs and OFTs in Brazilian agribusiness in terms of functional quality and dose application effectiveness (DAE). Seed samples treated by both processes were collected in 6 producing states of Brazil, Mato Grosso, Goiás, Paraná, Minas Gerais, São Paulo, and Santa Catarina. To evaluate the functional quality of the treatments, the coating percentage was determined by high-resolution image analysis with GroundEye® equipment, and the insecticide active ingredient DAE amount was determined with high-performance liquid chromatography (HPLC). Image analysis proved to be a promising technique to evaluate seed coating, in addition, samples treated with “on farm” technology had greater variation and heterogeneity in coating and active ingredient dose, while samples treated industrially showed greater homogeneity, uniformity, effectiveness of applied products. There was a strong positive correlation between coating with phytosanitary products and DAE in treated corn seeds, important parameters in the evaluation of the quality of the treatment.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67325155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-14DOI: 10.1590/2317-1545v45259022
Carlos Henrique Queiroz Rego, S. M. Cicero, F. Silva, Francisco Guilhien Gomes Júnior
Abstract: The occurrence of damage and empty space inside the seed can directly affect germination. The aim of this study was to evaluate the relationship between the occurrence of damage or empty space inside cowpea seeds and the physiological potential. In this study, seven lots of seeds of the cultivar ‘BRS Guariba’ were subjected to the X-ray test to identify damage by tissue deterioration, mechanical damage, damage by insects and malformed seeds and, later, to the germination test. The percentage of empty space between the embryonic area and the seed coat was determined from radiographic images in ImageJ software. Based on the results obtained, the seeds were classified into different categories, and the results were compared with the germination data. It was concluded that the analysis of radiographic images allowed the identification of damage and measurement of empty space in the internal cavity of the seeds, making it possible to establish a relationship between internal morphology and germination. The presence of damage, especially that caused by tissue deterioration or mechanical damage, depending on its location and intensity, has the potential to negatively affect the germination of cowpea seeds. Likewise, seeds that contain higher levels of empty space in their internal cavity tend not to germinate or to generate abnormal seedlings at the time of germination.
{"title":"Morphology and germination of Vigna unguiculata (L.) Walp. seeds","authors":"Carlos Henrique Queiroz Rego, S. M. Cicero, F. Silva, Francisco Guilhien Gomes Júnior","doi":"10.1590/2317-1545v45259022","DOIUrl":"https://doi.org/10.1590/2317-1545v45259022","url":null,"abstract":"Abstract: The occurrence of damage and empty space inside the seed can directly affect germination. The aim of this study was to evaluate the relationship between the occurrence of damage or empty space inside cowpea seeds and the physiological potential. In this study, seven lots of seeds of the cultivar ‘BRS Guariba’ were subjected to the X-ray test to identify damage by tissue deterioration, mechanical damage, damage by insects and malformed seeds and, later, to the germination test. The percentage of empty space between the embryonic area and the seed coat was determined from radiographic images in ImageJ software. Based on the results obtained, the seeds were classified into different categories, and the results were compared with the germination data. It was concluded that the analysis of radiographic images allowed the identification of damage and measurement of empty space in the internal cavity of the seeds, making it possible to establish a relationship between internal morphology and germination. The presence of damage, especially that caused by tissue deterioration or mechanical damage, depending on its location and intensity, has the potential to negatively affect the germination of cowpea seeds. Likewise, seeds that contain higher levels of empty space in their internal cavity tend not to germinate or to generate abnormal seedlings at the time of germination.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-14DOI: 10.1590/2317-1545v45266264
Milena Cristina Moraes, M. R. Bertão, C. C. Custódio, N. B. Machado-Neto
Abstract: Asymbiotic germination techniques are successful in species of the Catasetum genus. The diverse composition of the culture media can be critical; however, viable seeds are required for asymbiotic germination. The reliable method for viability assessment is the tetrazolium test. A decisive step in the efficiency of the tetrazolium test is the seeds preconditioning with sucrose solution, which has the potential to improve germination. Therefore, this work aimed to evaluate the seed germination of three species of the genus Catasetum in culture media, preconditioned or not with sucrose solution. Seeds of the species Catasetum osculatum, Ctsm. galeritum and Ctsm. complanatum were assessed by tetrazolium test and germinated in two different media, pretreated or not with 10% sucrose solution. The statistical analysis showed that the use of sucrose pretreatment significantly increased the germination rates of the species. We conclude that the pretreatment with 10% sucrose for 24 hours, regardless of the species and the culture medium, significantly increases the germination of Ctsm. complanatum, Ctsm. galeritum and Ctsm. osculatum seeds.
{"title":"The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae)","authors":"Milena Cristina Moraes, M. R. Bertão, C. C. Custódio, N. B. Machado-Neto","doi":"10.1590/2317-1545v45266264","DOIUrl":"https://doi.org/10.1590/2317-1545v45266264","url":null,"abstract":"Abstract: Asymbiotic germination techniques are successful in species of the Catasetum genus. The diverse composition of the culture media can be critical; however, viable seeds are required for asymbiotic germination. The reliable method for viability assessment is the tetrazolium test. A decisive step in the efficiency of the tetrazolium test is the seeds preconditioning with sucrose solution, which has the potential to improve germination. Therefore, this work aimed to evaluate the seed germination of three species of the genus Catasetum in culture media, preconditioned or not with sucrose solution. Seeds of the species Catasetum osculatum, Ctsm. galeritum and Ctsm. complanatum were assessed by tetrazolium test and germinated in two different media, pretreated or not with 10% sucrose solution. The statistical analysis showed that the use of sucrose pretreatment significantly increased the germination rates of the species. We conclude that the pretreatment with 10% sucrose for 24 hours, regardless of the species and the culture medium, significantly increases the germination of Ctsm. complanatum, Ctsm. galeritum and Ctsm. osculatum seeds.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-14DOI: 10.1590/2317-1545v45268445
S. A. D. N. Ferreira, D. Gentil, C. Ferreira
Abstract: Coccoloba gigantifolia fruits show uneven maturation within each raceme, which can affect the quality of the seeds. This study aimed to characterize racemes, fruits, and seeds, after harvest, and evaluate the physiological performance of the seeds as a function of the fruit maturation stage. After characterization, two experiments were set up. In the first, a completely randomized design was used, in a 4 (racemes) x 4 (fruit maturation stages: green, half-green, red, and purple) factorial scheme, with 4 replications. In the second, a randomized block design was adopted, in a 4 (fruit maturation stages) x 2 (with and without seed drying) factorial scheme, with 4 replications. Fruit maturation occurs progressively within each raceme, with fruits from completely green to completely purple in color existing at the same time. In propagation, preference should be given to harvesting racemes with a greater number of fruits in the half-green, red, and purple stages, since seeds from green fruits have low physiological quality. In addition to the variation in emergence as a function of fruit maturation, the seeds of the different racemes also showed different behaviors, which seems to be associated with different levels of seed dormancy.
{"title":"Emergence of Coccoloba gigantifolia Melo, Cid Ferreira & Gribel seedlings as a function of raceme and fruit maturation","authors":"S. A. D. N. Ferreira, D. Gentil, C. Ferreira","doi":"10.1590/2317-1545v45268445","DOIUrl":"https://doi.org/10.1590/2317-1545v45268445","url":null,"abstract":"Abstract: Coccoloba gigantifolia fruits show uneven maturation within each raceme, which can affect the quality of the seeds. This study aimed to characterize racemes, fruits, and seeds, after harvest, and evaluate the physiological performance of the seeds as a function of the fruit maturation stage. After characterization, two experiments were set up. In the first, a completely randomized design was used, in a 4 (racemes) x 4 (fruit maturation stages: green, half-green, red, and purple) factorial scheme, with 4 replications. In the second, a randomized block design was adopted, in a 4 (fruit maturation stages) x 2 (with and without seed drying) factorial scheme, with 4 replications. Fruit maturation occurs progressively within each raceme, with fruits from completely green to completely purple in color existing at the same time. In propagation, preference should be given to harvesting racemes with a greater number of fruits in the half-green, red, and purple stages, since seeds from green fruits have low physiological quality. In addition to the variation in emergence as a function of fruit maturation, the seeds of the different racemes also showed different behaviors, which seems to be associated with different levels of seed dormancy.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-13DOI: 10.1590/2317-1545v45267349
A. S. Silveira, D. T. Pinheiro, R. Oliveira, D. Dias, L. J. Silva
Abstract: Tomato is a crop sensitive to water deficit and the responses of seeds to germination under these conditions involve biochemical, physiological, and molecular processes. The aim of this study was to evaluate the physical, physiological, and biochemical changes in tomato seeds osmoprimed with selenium (Se) and subjected to water deficit during germination. Tomato seeds of the LA 4050 and LA 3475 accessions were osmoprimed with PEG 6000 solution (-1.0 MPa) plus Na2SeO4 (12.5 μM) or only with PEG 6000 solution for 24 h or unprimed. The seeds from the different treatments were subjected to water deficit (-0.3 MPa) or to control (0 MPa). Image analyses were performed (X-ray), physiological variables were obtained by the germination test and activities of SOD, CAT and POX enzymes were determined. Osmopriming, especially with PEG, provided the seeds of both accessions with higher densities and gray mean when compared to unprimed ones. Seeds under water deficit showed lower physiological performance and lower enzymatic activity. Se did not induce seed resistance to water deficit. Osmopriming with PEG 6000 at -1.0 MPa is an efficient technique for improving the physiological quality of seeds of tomato accessions susceptible and tolerant to water deficit. Moreover, the antioxidant enzyme CAT proved to be an indicator of oxidative stress tolerance in tomato seedlings under water deficit.
{"title":"Osmopriming with selenium: physical and physiological quality of tomato seeds in response to water deficit","authors":"A. S. Silveira, D. T. Pinheiro, R. Oliveira, D. Dias, L. J. Silva","doi":"10.1590/2317-1545v45267349","DOIUrl":"https://doi.org/10.1590/2317-1545v45267349","url":null,"abstract":"Abstract: Tomato is a crop sensitive to water deficit and the responses of seeds to germination under these conditions involve biochemical, physiological, and molecular processes. The aim of this study was to evaluate the physical, physiological, and biochemical changes in tomato seeds osmoprimed with selenium (Se) and subjected to water deficit during germination. Tomato seeds of the LA 4050 and LA 3475 accessions were osmoprimed with PEG 6000 solution (-1.0 MPa) plus Na2SeO4 (12.5 μM) or only with PEG 6000 solution for 24 h or unprimed. The seeds from the different treatments were subjected to water deficit (-0.3 MPa) or to control (0 MPa). Image analyses were performed (X-ray), physiological variables were obtained by the germination test and activities of SOD, CAT and POX enzymes were determined. Osmopriming, especially with PEG, provided the seeds of both accessions with higher densities and gray mean when compared to unprimed ones. Seeds under water deficit showed lower physiological performance and lower enzymatic activity. Se did not induce seed resistance to water deficit. Osmopriming with PEG 6000 at -1.0 MPa is an efficient technique for improving the physiological quality of seeds of tomato accessions susceptible and tolerant to water deficit. Moreover, the antioxidant enzyme CAT proved to be an indicator of oxidative stress tolerance in tomato seedlings under water deficit.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.1590/2317-1545v45259694
Débora Bueno Bernardo Pinto, Eduara Ferreira, F. A. Henning, H. Amaral, M. Hungria, M. Nogueira
Abstract: Farmers sometimes sow soybean (Glycine max (L.) Merrill) in dry soil in the expectation of rain in the short time. However, the forecast may not confirm, letting the inoculated seeds in the dry soil indefinitely. We assessed the survival of inoculated Bradyrhizobium and physiological quality of soybean seeds sown in dry soil. In the first experiment, irrigation was applied with 2 h, 1, 4, 11, 18, or 21 days after sowing; in the second experiment, sowing was carried out 2 h, 1, 5, 12, 14, or 20 days before irrigation. Each time represented a treatment in a completely randomized design. Bradyrhizobium cells dropped from ~8-9 × 104 colony forming units per seed soon after inoculation to -60% at 2 h after sowing in dry soil, and decreased to close to zero with time in both experiments. Although there was no effect on germination (59% and 81% in the first and second experiments, respectively), the exposure to dry soil reduced the emergence speed index from 19.5 (2 h) to 12.0 (21 days) in the first experiment and from 37.8 (2 h) to 13.8 (21 days) in the second. In the first experiment, the number of abnormal seedlings increased from 7% (2 h) to 24% (21 days); in the second, cotyledons showed cracks, which increased from 1% (2 h) to ~50% (≥ 5 days). Sowing in dry soil negatively affects not only the inoculated Bradyrhizobium, but also the physiological quality of soybean seeds.
{"title":"Recovery of Bradyrhizobium cells and effects on the physiological quality of soybean seeds sown in dry soil","authors":"Débora Bueno Bernardo Pinto, Eduara Ferreira, F. A. Henning, H. Amaral, M. Hungria, M. Nogueira","doi":"10.1590/2317-1545v45259694","DOIUrl":"https://doi.org/10.1590/2317-1545v45259694","url":null,"abstract":"Abstract: Farmers sometimes sow soybean (Glycine max (L.) Merrill) in dry soil in the expectation of rain in the short time. However, the forecast may not confirm, letting the inoculated seeds in the dry soil indefinitely. We assessed the survival of inoculated Bradyrhizobium and physiological quality of soybean seeds sown in dry soil. In the first experiment, irrigation was applied with 2 h, 1, 4, 11, 18, or 21 days after sowing; in the second experiment, sowing was carried out 2 h, 1, 5, 12, 14, or 20 days before irrigation. Each time represented a treatment in a completely randomized design. Bradyrhizobium cells dropped from ~8-9 × 104 colony forming units per seed soon after inoculation to -60% at 2 h after sowing in dry soil, and decreased to close to zero with time in both experiments. Although there was no effect on germination (59% and 81% in the first and second experiments, respectively), the exposure to dry soil reduced the emergence speed index from 19.5 (2 h) to 12.0 (21 days) in the first experiment and from 37.8 (2 h) to 13.8 (21 days) in the second. In the first experiment, the number of abnormal seedlings increased from 7% (2 h) to 24% (21 days); in the second, cotyledons showed cracks, which increased from 1% (2 h) to ~50% (≥ 5 days). Sowing in dry soil negatively affects not only the inoculated Bradyrhizobium, but also the physiological quality of soybean seeds.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.1590/2317-1545v45265346
Cinthia Vieira Golfi Andriazzi, D. K. Rocha, C. C. Custódio
Abstract: The use of infrared equipment to evaluate the physiological quality of seeds has become an extremely important technique for the operation, since it is not destructive to the seed and is safe for the operator. The experiment was divided into two stages, in the first, the controlled deterioration curve was carried out, and in the second stage, the validation of the NIR XDS process was carried out, through modeling for classification of the lots. The technique used for controlled deterioration proved to be efficient to show that with a higher band ratio index, the spectrum has a greater amplitude and lower physiological potential, the opposite is also valid, when the band index is smaller, the spectrum amplitude is smaller and higher values of germination and vigor were observed. For the second stage, it was concluded that spectra generated by NIR XDS equipment can be used quickly for decision making on corn seed samples, considering a binary classification for the parameters of germination and vigor according to the approval and disapproval values of lots considered in this study.
{"title":"Determination of the physiological quality of corn seeds by infrared equipment","authors":"Cinthia Vieira Golfi Andriazzi, D. K. Rocha, C. C. Custódio","doi":"10.1590/2317-1545v45265346","DOIUrl":"https://doi.org/10.1590/2317-1545v45265346","url":null,"abstract":"Abstract: The use of infrared equipment to evaluate the physiological quality of seeds has become an extremely important technique for the operation, since it is not destructive to the seed and is safe for the operator. The experiment was divided into two stages, in the first, the controlled deterioration curve was carried out, and in the second stage, the validation of the NIR XDS process was carried out, through modeling for classification of the lots. The technique used for controlled deterioration proved to be efficient to show that with a higher band ratio index, the spectrum has a greater amplitude and lower physiological potential, the opposite is also valid, when the band index is smaller, the spectrum amplitude is smaller and higher values of germination and vigor were observed. For the second stage, it was concluded that spectra generated by NIR XDS equipment can be used quickly for decision making on corn seed samples, considering a binary classification for the parameters of germination and vigor according to the approval and disapproval values of lots considered in this study.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.1590/2317-1545v45257617
Artur Sousa Silva, S. M. Cicero, F. F. C. Silva, F. G. Gomes‐Junior
Abstract: Image analysis techniques are expanding in agriculture and, for being fast, simple, and not destroying samples, they are interesting alternatives in the detection of immature rice seeds. Therefore, the aim of this study was to evaluate the quality of rice seeds using X-ray, multispectral and chlorophyll fluorescence image analysis techniques. Initially, with the seeds identified and numbered, radiographic images were obtained to determine the free space (area between the endosperm + embryo and the glumes). Subsequently, with the same seeds used in the X-ray, multispectral and chlorophyll fluorescence images were acquired, and then the computerized seedling analysis was performed with the SVIS® software. It was concluded that rice seeds that do not germinate or originate abnormal seedlings have free space equal to or greater than 18.68%, higher reflectance in the spectral bands with wavelength from 590 nm to 780 nm (41.46% to 64.21%, respectively) and in the band of 970 nm (75.65%), in addition to having chlorophyll fluorescence values equal to or greater than 40.58 and 112.92 at excitation/emission energies of 630/700 nm and 645/700 nm, respectively.
{"title":"X-ray, multispectral and chlorophyll fluorescence images: innovative methods for evaluating the physiological potential of rice seeds","authors":"Artur Sousa Silva, S. M. Cicero, F. F. C. Silva, F. G. Gomes‐Junior","doi":"10.1590/2317-1545v45257617","DOIUrl":"https://doi.org/10.1590/2317-1545v45257617","url":null,"abstract":"Abstract: Image analysis techniques are expanding in agriculture and, for being fast, simple, and not destroying samples, they are interesting alternatives in the detection of immature rice seeds. Therefore, the aim of this study was to evaluate the quality of rice seeds using X-ray, multispectral and chlorophyll fluorescence image analysis techniques. Initially, with the seeds identified and numbered, radiographic images were obtained to determine the free space (area between the endosperm + embryo and the glumes). Subsequently, with the same seeds used in the X-ray, multispectral and chlorophyll fluorescence images were acquired, and then the computerized seedling analysis was performed with the SVIS® software. It was concluded that rice seeds that do not germinate or originate abnormal seedlings have free space equal to or greater than 18.68%, higher reflectance in the spectral bands with wavelength from 590 nm to 780 nm (41.46% to 64.21%, respectively) and in the band of 970 nm (75.65%), in addition to having chlorophyll fluorescence values equal to or greater than 40.58 and 112.92 at excitation/emission energies of 630/700 nm and 645/700 nm, respectively.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.1590/2317-1545v45268562
F. C. Krzyzanowski, J. França-Neto, F. A. Henning
Abstract: The effects of lignin on soybean seed quality have been studied since the early 1990s. Initially, higher lignin content in the seed coat was associated with greater resistance to mechanical damage by the seeds. Later, positive relationships of lignin content in the seed coat were associated with tolerance to stresses caused by weathering in pre-harvest, resulting in improved seed quality. Additional studies have shown that higher lignin content in the pod wall of soybean is also related to production of seeds with high physiological and sanitary qualities. Colored soybean seeds (black or brown) are known to have higher seed quality due to higher lignin content in the seed coat; in addition, the presence of anthocyanin in the seed coat of colored seeds also contributes to improve seed quality. Finally, the effects of boron on lignin synthesis are also highlighted in this review article. As demonstrated in this review article, lignin content in the seed coat and pod wall of soybean plays an important role in relation to physical, health, and physiological seed qualities. These parameters should be considered in studies related to evaluation of the quality of soybean seeds as affected by genetic factors. These parameters should also be strongly considered for inclusion in breeding programs to improve soybean seed and grain quality.
{"title":"Importance of the lignin content in the pod wall and seed coat on soybean seed physiological and health performances","authors":"F. C. Krzyzanowski, J. França-Neto, F. A. Henning","doi":"10.1590/2317-1545v45268562","DOIUrl":"https://doi.org/10.1590/2317-1545v45268562","url":null,"abstract":"Abstract: The effects of lignin on soybean seed quality have been studied since the early 1990s. Initially, higher lignin content in the seed coat was associated with greater resistance to mechanical damage by the seeds. Later, positive relationships of lignin content in the seed coat were associated with tolerance to stresses caused by weathering in pre-harvest, resulting in improved seed quality. Additional studies have shown that higher lignin content in the pod wall of soybean is also related to production of seeds with high physiological and sanitary qualities. Colored soybean seeds (black or brown) are known to have higher seed quality due to higher lignin content in the seed coat; in addition, the presence of anthocyanin in the seed coat of colored seeds also contributes to improve seed quality. Finally, the effects of boron on lignin synthesis are also highlighted in this review article. As demonstrated in this review article, lignin content in the seed coat and pod wall of soybean plays an important role in relation to physical, health, and physiological seed qualities. These parameters should be considered in studies related to evaluation of the quality of soybean seeds as affected by genetic factors. These parameters should also be strongly considered for inclusion in breeding programs to improve soybean seed and grain quality.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67325087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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