Pub Date : 2023-01-27DOI: 10.1590/2317-1545v45265644
H. C. R. Catão, S. C. Hurtado
Abstract: Precision Agriculture considers soil attributes and production aspects to define management zones. Although there is little information, it is likely that seeds produced in different management zones have different quality after chemical treatment and storage. The aim was to evaluate the physiological quality of sorghum seeds produced in different management zones, after chemical treatment and storage. The management zones were defined from interpolated maps of soil and vegetation attributes with the aid of the Smart Map - SMP plugin, in a Qgis environment. After harvest, the seeds were treated with Thiamethoxam, Imidacloprid + Thiodicarb, Fludioxonil + Metalaxyl-M and a control treatment (water). Then, they were stored for 0, 60 and 120 days. The seeds were evaluated for their quality through the following tests: moisture, germination, dry mass of seedlings, electrical conductivity, and accelerated aging. The design was completely randomized in a 4 × 3 factorial scheme, with the management zones being analyzed independently. Sorghum seed treatments with insecticides cause greater phytotoxicity during storage in both management zones, unlike the fungicide treatment. Sorghum seeds produced in high-management zones have high physiological quality and less deterioration during storage. Sorghum seeds produced in low-management zones treated with insecticides, as the storage time increases, show lower vigor due to deterioration and phytotoxicity.
{"title":"Chemical treatment and storage of sorghum seeds produced in different management zones","authors":"H. C. R. Catão, S. C. Hurtado","doi":"10.1590/2317-1545v45265644","DOIUrl":"https://doi.org/10.1590/2317-1545v45265644","url":null,"abstract":"Abstract: Precision Agriculture considers soil attributes and production aspects to define management zones. Although there is little information, it is likely that seeds produced in different management zones have different quality after chemical treatment and storage. The aim was to evaluate the physiological quality of sorghum seeds produced in different management zones, after chemical treatment and storage. The management zones were defined from interpolated maps of soil and vegetation attributes with the aid of the Smart Map - SMP plugin, in a Qgis environment. After harvest, the seeds were treated with Thiamethoxam, Imidacloprid + Thiodicarb, Fludioxonil + Metalaxyl-M and a control treatment (water). Then, they were stored for 0, 60 and 120 days. The seeds were evaluated for their quality through the following tests: moisture, germination, dry mass of seedlings, electrical conductivity, and accelerated aging. The design was completely randomized in a 4 × 3 factorial scheme, with the management zones being analyzed independently. Sorghum seed treatments with insecticides cause greater phytotoxicity during storage in both management zones, unlike the fungicide treatment. Sorghum seeds produced in high-management zones have high physiological quality and less deterioration during storage. Sorghum seeds produced in low-management zones treated with insecticides, as the storage time increases, show lower vigor due to deterioration and phytotoxicity.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.1590/2317-1545v45262942
R. F. Santos, H. F. Placido, L. Lara, Hugo Zeni Neto, F. A. Henning, A. Braccini
Abstract: The aim of this study was to evaluate the isolated effect and the combination of products in the chemical treatment on the physiological potential of soybean seeds with different vigor levels, after different storage periods. The study was divided into four experiments, with different storage periods: 0, 45, 90 and 135 days, all with a completely randomized design, with four replicates. The treatments were arranged in a 12 x 2 (A x B) factorial scheme, in which factor A consisted of the application of seed treatment and factor B consisted of initial vigor levels. The physiological potential of the seeds was determined by means of the following evaluations: first germination count, seed germination, seedling emergence in the sand substrate, accelerated aging with NaCl-saturated solution, vigor index and average seedling length (Vigor-S®). Storage of seeds under uncontrolled conditions causes greater deterioration, and this process can be intensified by previous chemical treatment of the seeds. Among the products studied, the use of drying powder alone was the treatment that caused the greatest losses in the different situations studied. To ensure the maintenance of the physiological potential of soybean seeds, treatment should be carried out as close as possible to sowing.
摘要:本试验旨在评价化学处理对不同活力水平、不同贮藏期大豆种子生理电位的单独效应及组合效应。试验分为4个试验，贮存期分别为0、45、90和135 d，采用完全随机设计，共4个重复。处理按12 × 2 (a × B)因子方案安排，其中因子a为施用种子处理，因子B为初始活力水平。通过以下评价来确定种子的生理电位:首次发芽计数、种子萌发、在砂基质中出苗、nacl饱和溶液加速老化、活力指数和平均幼苗长度(vigor -s®)。在不受控制的条件下储存种子会导致更大的变质，而这一过程可以通过事先对种子进行化学处理而加剧。在研究的产品中，在研究的不同情况下，单独使用干粉是造成最大损失的处理。为保证大豆种子生理电位的维持，处理应尽量在播种前进行。
{"title":"Physiological potential of soybean seeds treated and stored under uncontrolled conditions","authors":"R. F. Santos, H. F. Placido, L. Lara, Hugo Zeni Neto, F. A. Henning, A. Braccini","doi":"10.1590/2317-1545v45262942","DOIUrl":"https://doi.org/10.1590/2317-1545v45262942","url":null,"abstract":"Abstract: The aim of this study was to evaluate the isolated effect and the combination of products in the chemical treatment on the physiological potential of soybean seeds with different vigor levels, after different storage periods. The study was divided into four experiments, with different storage periods: 0, 45, 90 and 135 days, all with a completely randomized design, with four replicates. The treatments were arranged in a 12 x 2 (A x B) factorial scheme, in which factor A consisted of the application of seed treatment and factor B consisted of initial vigor levels. The physiological potential of the seeds was determined by means of the following evaluations: first germination count, seed germination, seedling emergence in the sand substrate, accelerated aging with NaCl-saturated solution, vigor index and average seedling length (Vigor-S®). Storage of seeds under uncontrolled conditions causes greater deterioration, and this process can be intensified by previous chemical treatment of the seeds. Among the products studied, the use of drying powder alone was the treatment that caused the greatest losses in the different situations studied. To ensure the maintenance of the physiological potential of soybean seeds, treatment should be carried out as close as possible to sowing.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/2317-1545v45274600
Lisha Fang, Mengxing Zhang, Weiwei Liu, Zhen Liu, Li Dai, Yanmei Wang
Abstract: The aim of this study was to investigate the effects of Idesia polycarpa fruit blackening on fruit and seed morphological characteristics, oil content, fatty acid content, seed germination rate and physiological properties. Germination tests were conducted under dry and wet storage at 5 °C for 0 d, 20 d, 40 d, 60 d and 80 d. The fruit mass, the 100-grain weight, the moisture content, the oil content of seeds and oleic acid in unsaturated fatty acids of black fruit are significantly lower than red fruit (P < 0.05). The germination rate of black fruit seeds was higher than red under wet storage and the malondialdehyde content of black fruits decreased with increasing storage time. Our results demonstrated that black fruits of I. polycarpa should not be discarded indiscriminately and that the color of the fruits can be chosen according to the purpose of use. Black fruits are picked for species propagation, while red fruits are mainly harvested for oil extraction.
{"title":"Influence of fruit color on the oil quality and seed germination of Idesia polycarpa Maxim.","authors":"Lisha Fang, Mengxing Zhang, Weiwei Liu, Zhen Liu, Li Dai, Yanmei Wang","doi":"10.1590/2317-1545v45274600","DOIUrl":"https://doi.org/10.1590/2317-1545v45274600","url":null,"abstract":"Abstract: The aim of this study was to investigate the effects of Idesia polycarpa fruit blackening on fruit and seed morphological characteristics, oil content, fatty acid content, seed germination rate and physiological properties. Germination tests were conducted under dry and wet storage at 5 °C for 0 d, 20 d, 40 d, 60 d and 80 d. The fruit mass, the 100-grain weight, the moisture content, the oil content of seeds and oleic acid in unsaturated fatty acids of black fruit are significantly lower than red fruit (P < 0.05). The germination rate of black fruit seeds was higher than red under wet storage and the malondialdehyde content of black fruits decreased with increasing storage time. Our results demonstrated that black fruits of I. polycarpa should not be discarded indiscriminately and that the color of the fruits can be chosen according to the purpose of use. Black fruits are picked for species propagation, while red fruits are mainly harvested for oil extraction.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135609261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/2317-1545v45277021
Lucas Rodrigues de Souza, Marcelo Augusto Rocha Limão, Daniel Teixeira Pinheiro, Gabriel Cordeiro de Oliveira Peris, Denise Cunha Fernandes dos Santos Dias, Luiz Antônio dos Santos Dias
Abstract: The demand for techniques that make it possible to assess the physiological potential of seeds quickly and assertively make near-infrared spectroscopy (FT-NIR) and seedling analysis using ILASTIK software promising tools. The aim of this study was to evaluate the physiological potential of Urochloa decumbens seeds using near-infrared spectroscopy (FT-NIR) and ILASTIK software. Seeds from 10 lots of U. decumbens were classified according to their physiological potential (germination and vigor) and then FT-NIR spectra were obtained from individual seed samples, the original spectra being pre-processed with different dispersion correction methods used for the construction of a classification model through partial least squares discriminant analysis (PSL-DA). For the ILASTIK evaluation, the seedlings were photographed at 7 and 14 days of germination and the trained classifier was applied to the images, generating data on the numbers of strong seedlings, weak seedlings and non-germinated seeds. With data from the FT-NIR technique pre-processed by the 2nd derivative of Savitzky-Golay, it was possible to obtain a classification model with high efficiency to discriminate the classes regarding the physiological potential of the seeds. ILASTIK was efficient to classify seeds according to their physiological potential after only 7 days of germination. FT-NIR and ILASTIK analyses are non-destructive and fast alternatives, with great potential for quality control of U. decumbens seed lots.
{"title":"Near infrared spectroscopy and seedling image analysis to evaluate the physiological potential of Urochloa decumbens (Stapf) R.D. Webster seeds","authors":"Lucas Rodrigues de Souza, Marcelo Augusto Rocha Limão, Daniel Teixeira Pinheiro, Gabriel Cordeiro de Oliveira Peris, Denise Cunha Fernandes dos Santos Dias, Luiz Antônio dos Santos Dias","doi":"10.1590/2317-1545v45277021","DOIUrl":"https://doi.org/10.1590/2317-1545v45277021","url":null,"abstract":"Abstract: The demand for techniques that make it possible to assess the physiological potential of seeds quickly and assertively make near-infrared spectroscopy (FT-NIR) and seedling analysis using ILASTIK software promising tools. The aim of this study was to evaluate the physiological potential of Urochloa decumbens seeds using near-infrared spectroscopy (FT-NIR) and ILASTIK software. Seeds from 10 lots of U. decumbens were classified according to their physiological potential (germination and vigor) and then FT-NIR spectra were obtained from individual seed samples, the original spectra being pre-processed with different dispersion correction methods used for the construction of a classification model through partial least squares discriminant analysis (PSL-DA). For the ILASTIK evaluation, the seedlings were photographed at 7 and 14 days of germination and the trained classifier was applied to the images, generating data on the numbers of strong seedlings, weak seedlings and non-germinated seeds. With data from the FT-NIR technique pre-processed by the 2nd derivative of Savitzky-Golay, it was possible to obtain a classification model with high efficiency to discriminate the classes regarding the physiological potential of the seeds. ILASTIK was efficient to classify seeds according to their physiological potential after only 7 days of germination. FT-NIR and ILASTIK analyses are non-destructive and fast alternatives, with great potential for quality control of U. decumbens seed lots.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135105015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/2317-1545v45274866
Jiyue Wang, Jiajun Xu, Mingli Yang, Mingzhi Miao
Abstract: A comparative study for evaluating the influence of metal oxide nanoparticles (MONPs) (Al2O3, TiO2, and SiO2) on seed germination and seedling growth in okra was implemented under water deficit stress stimulated by PEG-6000. The results revealed that additive of SiO2 nanoparticles (SNPs) and Al2O3 (ANPs) have significant positive effect on seeds germination, while a reduction of that were observed by using TiO2 nanoparticles (TNPs) at 50 mg.L-1 concentration under 5% PEG-6000 stimulated drought stress as compared to that without MONPs. Further, relative to non-MONPs treatment, all the tested MONPs dramatically enhanced the drought stress tolerance in okra seedlings due to the improvement of morphological growth despite a few traits were inhibited under certain water deficiency to some degree. Improvement of drought resistance induced by TNPs and ANPs were found to be higher than that by SNPs. Our finding provides a promising approach to cope with water scarcity, as application of metal oxide nanoparticles to be a potential option to protect okra plants against drought stress.
{"title":"Influence of SiO2, Al2O3 and TiO2 nanoparticles on okra seed germination under PEG-6000 simulated water deficit stress","authors":"Jiyue Wang, Jiajun Xu, Mingli Yang, Mingzhi Miao","doi":"10.1590/2317-1545v45274866","DOIUrl":"https://doi.org/10.1590/2317-1545v45274866","url":null,"abstract":"Abstract: A comparative study for evaluating the influence of metal oxide nanoparticles (MONPs) (Al2O3, TiO2, and SiO2) on seed germination and seedling growth in okra was implemented under water deficit stress stimulated by PEG-6000. The results revealed that additive of SiO2 nanoparticles (SNPs) and Al2O3 (ANPs) have significant positive effect on seeds germination, while a reduction of that were observed by using TiO2 nanoparticles (TNPs) at 50 mg.L-1 concentration under 5% PEG-6000 stimulated drought stress as compared to that without MONPs. Further, relative to non-MONPs treatment, all the tested MONPs dramatically enhanced the drought stress tolerance in okra seedlings due to the improvement of morphological growth despite a few traits were inhibited under certain water deficiency to some degree. Improvement of drought resistance induced by TNPs and ANPs were found to be higher than that by SNPs. Our finding provides a promising approach to cope with water scarcity, as application of metal oxide nanoparticles to be a potential option to protect okra plants against drought stress.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135501765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/2317-1545v45268805
Priscila Carvalho da Silva, Natalia Dias Lima, Tathiana Elisa Masetto, Manoel Carlos Gonçalves, Liliam Silvia Cândido
Abstract: Knowledge of the genetic variability and of the variables for evaluating common bean lines for the physiological seed quality trait is important for the selection of promising common bean genotypes for this trait in breeding programs. Through multivariate analysis, the objective of this study was to evaluate the efficiency of physical and physiological tests for studies of genetic variability in seeds of common bean lines. Twenty lines of common bean were evaluated for thousand-seed weight, first count, germination, tetrazolium, electrical conductivity, accelerated aging, field emergence and seedling performance. The experimental data were subjected to analysis of variance, analysis of genetic parameters and multivariate analysis of principal components. Genetic variability was observed among the common bean lines, and two groups of lines with superior physiological performance according to the germination and seed vigor tests were identified. First germination count, germination, seedling length in the field, accelerated aging and emergence speed index tests are the most promising for characterizing the physiological potential of seeds of common bean lines.
{"title":"Multivariate analysis applied to the evaluation of genetic variability for the physiological quality trait of common bean seeds","authors":"Priscila Carvalho da Silva, Natalia Dias Lima, Tathiana Elisa Masetto, Manoel Carlos Gonçalves, Liliam Silvia Cândido","doi":"10.1590/2317-1545v45268805","DOIUrl":"https://doi.org/10.1590/2317-1545v45268805","url":null,"abstract":"Abstract: Knowledge of the genetic variability and of the variables for evaluating common bean lines for the physiological seed quality trait is important for the selection of promising common bean genotypes for this trait in breeding programs. Through multivariate analysis, the objective of this study was to evaluate the efficiency of physical and physiological tests for studies of genetic variability in seeds of common bean lines. Twenty lines of common bean were evaluated for thousand-seed weight, first count, germination, tetrazolium, electrical conductivity, accelerated aging, field emergence and seedling performance. The experimental data were subjected to analysis of variance, analysis of genetic parameters and multivariate analysis of principal components. Genetic variability was observed among the common bean lines, and two groups of lines with superior physiological performance according to the germination and seed vigor tests were identified. First germination count, germination, seedling length in the field, accelerated aging and emergence speed index tests are the most promising for characterizing the physiological potential of seeds of common bean lines.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135101762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/2317-1545v45266896
Nasma Henriqueta Da Sorte Cossa, Everson Reis Carvalho, Juliana Maria Espíndola Lima, Luiz Fernando de Souza Moraes, Felisberto Venâncio Chicamasso Miquicene, Ivan Natividade Júlio Zevo
Abstract: The objective of this study was to assess the impact of incorporating vermiculite into germination tests for both chemically treated and untreated stored cotton seeds. To that end, two independent experiments were conducted using cotton seeds of the cultivar DP1746B2RF under chemical treatment with a mixture of fungicide, insecticides, and nematicide; and both experiments were set up in a completely randomized design with four replications. In Experiment 1, the objective was to determine the ideal water volume for moistening the vermiculite. Four water volumes were used: 1.5, 2.0, 2.5, and 3.0 times the weight of the dry vermiculite. Experiment 2 was conducted in a 2 × 2 × 2 triple factorial arrangement consisting of two substrates (germitest paper and germitest paper + vermiculite), two treatments (chemically treated seeds and untreated seeds), and two storage periods (0 and 150 days). First germination count and germination were evaluated in the two experiments. From the results found in Experiment 1, the water volume corresponding to 3.0 times the weight of the vermiculite provided the best conditions for germination. In Experiment 2, the most appropriate substrate for conducting the germination test of untreated and chemically treated cotton seeds is vermiculite, which led to rapid germination and uniform seedling development.
{"title":"An alternative approach to conducting germination tests on chemically treated and untreated stored cotton seeds","authors":"Nasma Henriqueta Da Sorte Cossa, Everson Reis Carvalho, Juliana Maria Espíndola Lima, Luiz Fernando de Souza Moraes, Felisberto Venâncio Chicamasso Miquicene, Ivan Natividade Júlio Zevo","doi":"10.1590/2317-1545v45266896","DOIUrl":"https://doi.org/10.1590/2317-1545v45266896","url":null,"abstract":"Abstract: The objective of this study was to assess the impact of incorporating vermiculite into germination tests for both chemically treated and untreated stored cotton seeds. To that end, two independent experiments were conducted using cotton seeds of the cultivar DP1746B2RF under chemical treatment with a mixture of fungicide, insecticides, and nematicide; and both experiments were set up in a completely randomized design with four replications. In Experiment 1, the objective was to determine the ideal water volume for moistening the vermiculite. Four water volumes were used: 1.5, 2.0, 2.5, and 3.0 times the weight of the dry vermiculite. Experiment 2 was conducted in a 2 × 2 × 2 triple factorial arrangement consisting of two substrates (germitest paper and germitest paper + vermiculite), two treatments (chemically treated seeds and untreated seeds), and two storage periods (0 and 150 days). First germination count and germination were evaluated in the two experiments. From the results found in Experiment 1, the water volume corresponding to 3.0 times the weight of the vermiculite provided the best conditions for germination. In Experiment 2, the most appropriate substrate for conducting the germination test of untreated and chemically treated cotton seeds is vermiculite, which led to rapid germination and uniform seedling development.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"259 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135501332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/2317-1545v45274637
Ya Gao, Xin Pan, Fang Zeng, Chunmei Zheng, Wenxuan Ge, Yanxia Sun, Wenping Du, Xiaoyong Wu
Abstract: Quinoa has been recognized as the sole “comprehensive nutritional crop”; however, it is susceptible to pre-harvest sprouting (PHS). While quantitative reverse transcription polymerase chain reaction (RT-qPCR) has been extensively employed for gene expression level detection, the selection of suitable reference genes is imperative to ensure precise gene expression quantification across diverse conditions. This study aims to identify stable reference genes in quinoa seeds under ABA and GA, in order to provide a basis for subsequent research on PHS. Seeds were subjected to different concentrations of ABA and GA (10 μM, 50 μM, 100 μM, and 200 μM). The most suitable treatment concentration was determined based on seed viability. Here, MON1, GAPDH, EIF3, EF1α, ACT, TUB1, and TUB6 were selected as candidate genes. The suitability of these reference genes under different conditions was assessed using various methods including Ct values, geNorm, NormFinder, BestKeeper, Delta Ct, and RefFinder. Based on the results obtained from the hormone experiments, it was observed that the application of 100 μM ABA and 200 μM GA yielded the most advantageous outcomes. Additionally, the most appropriate reference genes for different treatments are ACT and TUB1 (H2O treatment), EIF3 and MON1 (ABA, GA treatment and also for the combined data set of the three groups). However, GAPDH exhibited the least stability across all treatments. In summary, ACT is recommended as the reference gene for natural quinoa germination, while EIF3 and MON1 should be used for ABA and GA treatments.
{"title":"The identification of suitable internal reference genes in quinoa seeds subjected to abscisic acid and gibberellin treatment","authors":"Ya Gao, Xin Pan, Fang Zeng, Chunmei Zheng, Wenxuan Ge, Yanxia Sun, Wenping Du, Xiaoyong Wu","doi":"10.1590/2317-1545v45274637","DOIUrl":"https://doi.org/10.1590/2317-1545v45274637","url":null,"abstract":"Abstract: Quinoa has been recognized as the sole “comprehensive nutritional crop”; however, it is susceptible to pre-harvest sprouting (PHS). While quantitative reverse transcription polymerase chain reaction (RT-qPCR) has been extensively employed for gene expression level detection, the selection of suitable reference genes is imperative to ensure precise gene expression quantification across diverse conditions. This study aims to identify stable reference genes in quinoa seeds under ABA and GA, in order to provide a basis for subsequent research on PHS. Seeds were subjected to different concentrations of ABA and GA (10 μM, 50 μM, 100 μM, and 200 μM). The most suitable treatment concentration was determined based on seed viability. Here, MON1, GAPDH, EIF3, EF1α, ACT, TUB1, and TUB6 were selected as candidate genes. The suitability of these reference genes under different conditions was assessed using various methods including Ct values, geNorm, NormFinder, BestKeeper, Delta Ct, and RefFinder. Based on the results obtained from the hormone experiments, it was observed that the application of 100 μM ABA and 200 μM GA yielded the most advantageous outcomes. Additionally, the most appropriate reference genes for different treatments are ACT and TUB1 (H2O treatment), EIF3 and MON1 (ABA, GA treatment and also for the combined data set of the three groups). However, GAPDH exhibited the least stability across all treatments. In summary, ACT is recommended as the reference gene for natural quinoa germination, while EIF3 and MON1 should be used for ABA and GA treatments.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135610212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/2317-1545v45273211
Thaís Valério Raimundo Menino, Breno Miguel Joia, Aline Marengoni Almeida, Francisco Carlos Krzyzanowski, Rogério Marchiosi, Osvaldo Ferrarese-Filho
Abstract: Soybean seeds are crucial for global food production. Various factors affect the quality of soybean seeds, including mechanical damage, which can lead to reduced germination potential and decreased seedling vigor. The presence of lignin in the seed coat contributes to resistance to mechanical damage. However, the relationship between the monomeric composition of lignin and mechanical damage is unknown. To fill this gap, we evaluated the contents of monomers hydroxyphenyl (H), guaiacyl (G), and syringyl (S) in seed coats of three cultivars of soybean, namely, Doko, IAS-5, and Savana. The results revealed that the monomeric composition of lignin varied between resistant and susceptible cultivars. The levels of G and S monomers were inverse in the cultivars Doko and Savana, suggesting that the composition of lignin monomers may play a crucial role in the resistance of soybean seeds to mechanical damage. In addition, negative linear regressions between lignin and S monomer contents and S/G ratios could be helpful as an alternative to identify resistance in soybean seeds.
{"title":"Lignin monomeric composition in soybean seed coats and resistance to mechanical damage","authors":"Thaís Valério Raimundo Menino, Breno Miguel Joia, Aline Marengoni Almeida, Francisco Carlos Krzyzanowski, Rogério Marchiosi, Osvaldo Ferrarese-Filho","doi":"10.1590/2317-1545v45273211","DOIUrl":"https://doi.org/10.1590/2317-1545v45273211","url":null,"abstract":"Abstract: Soybean seeds are crucial for global food production. Various factors affect the quality of soybean seeds, including mechanical damage, which can lead to reduced germination potential and decreased seedling vigor. The presence of lignin in the seed coat contributes to resistance to mechanical damage. However, the relationship between the monomeric composition of lignin and mechanical damage is unknown. To fill this gap, we evaluated the contents of monomers hydroxyphenyl (H), guaiacyl (G), and syringyl (S) in seed coats of three cultivars of soybean, namely, Doko, IAS-5, and Savana. The results revealed that the monomeric composition of lignin varied between resistant and susceptible cultivars. The levels of G and S monomers were inverse in the cultivars Doko and Savana, suggesting that the composition of lignin monomers may play a crucial role in the resistance of soybean seeds to mechanical damage. In addition, negative linear regressions between lignin and S monomer contents and S/G ratios could be helpful as an alternative to identify resistance in soybean seeds.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135612064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/2317-1545v45272288
Eliza Maiara Nogueira de Sena, Fabricio Francisco Santos da Silva, Jailton de Jesus Silva, Raquel Araujo Gomes, Claudineia Regina Pelacani, Barbara França Dantas
Abstract: Erythrina velutina Willd is a native Fabaceae with wide occurrence, mainly in the Caatinga Domain with multiple uses. This study aimed to compare the vulnerability/tolerance of seeds of four accessions of E. velutina to osmotic stresses. Four replications were conducted with 25 seeds, germinated in osmotic solutions of NaCl or PEG up to -1.73 MPa. Seeds that did not germinate after 14 days were recovered in distilled water. Obtained data were fitted in Boltzmann curves and t50 and synchrony were evaluated. A probit analysis was performed, and hydrotime and halotime models were constructed. In distilled water, the germination curve was similar for all accessions. When the concentration of salt or PEG in the substrate increased, the accessions differentiated into two groups. Two accessions - Jutaí 2012 and Jutaí 2015 - maintained high germination up to -0.86 MPa NaCl, while Caboclo 2008 and Caiçara 2011 accessions presented a lower germination speed. The hydrotime and halotime analyses separated the germination response of accessions according to their physiological quality and tolerance to osmotic and saline conditions. Seeds viability was maintained at both stresses, since germination was reestablished at distilled water, thus constituting a crucial response for this species’ survival and conservation.
{"title":"Germination niche of a neotropical dry forest species: seed osmotic stress and recovery","authors":"Eliza Maiara Nogueira de Sena, Fabricio Francisco Santos da Silva, Jailton de Jesus Silva, Raquel Araujo Gomes, Claudineia Regina Pelacani, Barbara França Dantas","doi":"10.1590/2317-1545v45272288","DOIUrl":"https://doi.org/10.1590/2317-1545v45272288","url":null,"abstract":"Abstract: Erythrina velutina Willd is a native Fabaceae with wide occurrence, mainly in the Caatinga Domain with multiple uses. This study aimed to compare the vulnerability/tolerance of seeds of four accessions of E. velutina to osmotic stresses. Four replications were conducted with 25 seeds, germinated in osmotic solutions of NaCl or PEG up to -1.73 MPa. Seeds that did not germinate after 14 days were recovered in distilled water. Obtained data were fitted in Boltzmann curves and t50 and synchrony were evaluated. A probit analysis was performed, and hydrotime and halotime models were constructed. In distilled water, the germination curve was similar for all accessions. When the concentration of salt or PEG in the substrate increased, the accessions differentiated into two groups. Two accessions - Jutaí 2012 and Jutaí 2015 - maintained high germination up to -0.86 MPa NaCl, while Caboclo 2008 and Caiçara 2011 accessions presented a lower germination speed. The hydrotime and halotime analyses separated the germination response of accessions according to their physiological quality and tolerance to osmotic and saline conditions. Seeds viability was maintained at both stresses, since germination was reestablished at distilled water, thus constituting a crucial response for this species’ survival and conservation.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135549290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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