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Biological control of sclerotial blight of tea using arbuscular mycorrhizal fungus and plant growth promoting rhizobacterium. 利用丛枝菌根真菌和促进植物生长的根瘤菌生物防治茶叶菌核枯萎病。
Pub Date : 2012-01-01 DOI: 10.20425/IJTS.V8I4.4709
B. Chakraborty, U. Chakraborty, U. De, A. Chakraborty
Glomus fasciculatum , one of the dominant arbuscular mycorrhizal fungus (AMF) associated with tea root colonization, was selected and maintained in maize plants. Besides AMF, Bacillus amyloliquefaciens TRS6 isolated from tea rhizosphere, which showed in vitro antagonism to a number of tea root rot pathogens as well as siderophore-producing and phosphate-solubilizing activities, was selected for utilization as bioinoculants in tea plants for improvement of health status. The bacterium was applied to the soil as aqueous suspensions, and in case of G. fasciculatum , soil was inoculated with its spores and in joint inoculations, the AMF was inoculated prior to the bacterium. Inoculation of rhizosphere of tea plants of six different varieties (TV-18, T-17, AV-2, T-78, UP-3 and UP-26) with any of two microorganisms increased growth of plants, but the most significant increase was obtained in dual application. Plant growth was measured in terms of increase in height, increase in number of branches and leaves. Similarly, sclerotial blight of tea, caused by Sclerotium rolfsu , was suppressed to certain extent by G. fasciculatum or B. amyloliquefaciens , but significant suppression occurred when G. fasciculatum and B. amyloliquefaciens were applied jointly. Polyphenolics and four major defense enzymes showed enhanced activities during disease suppression. Western blot of the enzjone extracts from control and all treated plants using PAb raised against chitinase revealed strong reaction when disease suppression was evident. Population of S. rolfsu in soil was also determined following immunological techniques using PAb raised against the pathogen. Results of ELISA and dot-blot revealed that application of G. fasciculatum and B. amyloliquefaciens significantly reduced S. rolfsu population.
束状真菌(Glomus fasciculatum)是一种与茶根定植相关的显性丛枝菌根真菌(AMF)。除AMF外,从茶根际分离的解淀粉芽孢杆菌TRS6对多种茶根腐病病原菌具有体外拮抗作用,并具有产铁载体和溶磷活性,可作为生物接种剂应用于茶树,改善茶树健康状况。将该细菌作为水悬浮液施用于土壤中,对于束状菌,将其孢子接种于土壤中,在联合接种时,AMF先于细菌接种。对6个不同品种(TV-18、T-17、AV-2、T-78、UP-3和UP-26)的茶树根际接种两种微生物中的任何一种都能促进植株的生长,但双施效果最显著。植物的生长是根据高度的增加、枝叶数量的增加来测量的。同样,束状芽孢菌和解淀粉芽孢菌对茶树菌核枯萎病也有一定的抑制作用,但束状芽孢菌和解淀粉芽孢菌联合施用对茶树菌核枯萎病的抑制作用显著。多酚类物质和四种主要防御酶在疾病抑制过程中表现出增强的活性。用PAb对几丁质酶培养的对照和处理植株的酶提物进行Western blot检测,结果显示,在疾病抑制明显的情况下,酶提物反应强烈。采用免疫技术,采用PAb对病原菌进行免疫培养,测定了土壤中rolfsu的种群数量。酶联免疫吸附和斑点免疫分析结果显示,束状梭菌和解淀粉芽孢杆菌均能显著降低大蠊种群数量。
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引用次数: 1
What Lies in the Future of Tea Science in the Era of Genomics 基因组时代的茶学未来在哪里
Pub Date : 2011-12-18 DOI: 10.5376/JTSR.2011.01.0001
James F. Vancouver
Human being has been using tea as a beverage for thousands of years. Chinese tea is made of dried leaves from tea plants ( Camellia sinensis (L.) Kuntze ), while the British tea, which is called herbal tea, is a mixture of dried herbs from a variety of plants. Countless plants are used to make tea, but do the active compounds in these plants serve the same functions as typical tea leaves do? In fact, it is not yet known what exactly a cup of herbal tea contains. With the development of biotechnology and the arrival of the era of genomics, what lies in the path in the future development of tea science? In this paper, the author would like to propose some directions as the following: 1) the exploration at molecular level of what is inside a tea bag; 2) the study of a typical tea plant ( Camellia sinensis ) as a model plant for tea study; 3) Tea-nomics study based on new-generation of sequencing technology; 4) comparative metabolomics studies on biologically active compounds in tea; 5) identification based on pharmacology and toxicology. With the advent of the genomics era, the mysteries in tea bags will slowly be deciphered; whether it is Chinese tea or English tea, tea will still be the most popular drink.
几千年来,人类一直把茶作为一种饮料。中国茶是由茶树(Camellia sinensis (L.))的干叶子制成的。而英国的茶,被称为凉茶,是由各种植物的干草药混合而成的。无数的植物被用来泡茶,但这些植物中的活性化合物是否与典型的茶叶具有相同的功能?事实上,我们还不知道一杯凉茶到底含有什么。随着生物技术的发展和基因组时代的到来,茶学未来的发展之路在哪里?在本文中,笔者提出了以下几个方向:1)在分子水平上对茶包内部的物质进行探索;2)研究一种典型茶树(Camellia sinensis)作为茶叶研究的模式植物;3)基于新一代测序技术的茶经济学研究;4)茶叶生物活性物质的比较代谢组学研究;5)基于药理学和毒理学的鉴定。随着基因组时代的到来,茶包里的谜团将慢慢被破译;无论是中国茶还是英国茶,茶仍然是最受欢迎的饮料。
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引用次数: 3
Characterization of the key aromatic constituents in tea flowers of elite Chinese tea cultivars 中国优良茶树品种茶花主要芳香成分的研究
Pub Date : 2006-01-01 DOI: 10.20425/IJTS.V6I1.4678
B. Han, P. Zhou, L. Cui, Jianyu Fu
The volatiles in flowers of twenty-three elite tea cultivars were obtained by simultaneous distillation extraction, from which seventy-nine aromatic constituents were identified using gas chromatography coupled with mass spectrometry. The major detected constituents found were acetophenone, linalool, 1-hexanol, 2-pentanol, methyl salicylate, 4-methyl-2-hexanone, alpha-methyl-benzene methanol, cis-linaloloxide, acetic acid, Z-3-Hexen-1-ol, S-2-heptanol, and hexanal. Both acetophenone and linalool were determined from every cultivar, and each volatile accounted for more than 20 % of the total. Several of the cultivars analysed contained some special constituents which were not detected from other cultivars.
采用同步蒸馏提取的方法获得了23个优质茶叶品种花中的挥发物,并利用气相色谱-质谱联用技术鉴定了79种芳香成分。检测到的主要成分有苯乙酮、芳樟醇、1-己醇、2-戊醇、水杨酸甲酯、4-甲基-2-己酮、-甲基苯甲醇、顺式芳樟醇、乙酸、z -3-己烯-1-醇、s- 2-庚醇和己醛。各品种均检测到苯乙酮和芳樟醇,各挥发物含量均在20%以上。分析的几个品种含有一些其他品种未检出的特殊成分。
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引用次数: 10
AVAILABLE SULPHUR STATUS OF DOOARS TEA SOILS 门茶土壤有效硫状况
Pub Date : 2006-01-01 DOI: 10.20425/IJTS.V5I3AND4.4680
J. Ghosh, U. George, N. Barpujari
Available sulphur was determined on 5077 soils from the tea growing areas of Dooars (North Bengal), received for routine soil testing between 2000-2003. Over 43% of the soils had more than the critical limit of 40 ppm available sulphur. A distinct geographical trend in sulphur availability was observed, with the four western sub-districts showing a greater frequency of high available sulphur than the three eastern sub-districts. As expected, increased organic carbon in the soils could be correlated by a quadratic equation to the available sulphur status. Three subdistricts, Chulsa, Binnaguri and Dalgaon did not show a high correlation between organic carbon and available sulphur. Surprisingly, a negative linear relationship could be observed between soil pH and available sulphur, in all but the Jainti sub-district. This may be compounded by a negative correlation between soil pH and organic carbon status, in all but the Binnaguri, Kalchini and Jainti sub-districts.
在2000-2003年期间接受的常规土壤测试中,对来自Dooars(北孟加拉)茶叶种植区的5077种土壤进行了有效硫测定。超过43%的土壤的有效硫含量超过了40 ppm的临界限值。硫的可用性在地理上有明显的趋势,西部四个分区的高可用性硫出现的频率高于东部三个分区。正如预期的那样,土壤中有机碳的增加可以通过二次方程与有效硫状态相关。丘尔沙、滨那里、达岗等3个街道的有机碳和有效硫的相关性不高。令人惊讶的是,土壤pH值和有效硫之间呈负线性关系,除了Jainti街道。除了Binnaguri、Kalchini和Jainti区外,土壤pH值和有机碳状况之间的负相关关系可能会加剧这种情况。
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引用次数: 2
WTO AND AGRICULTURE TRADING IN FOOD INSECURITY 世贸组织和粮食不安全的农业贸易
Pub Date : 2004-08-01 DOI: 10.20425/IJTS.V3I3AND4.4658
Devinder Sharma
For any tourist, Kerala, in down south India, is an attractive destination. The tropical climate and the unique backwater systems have added charm to its pristine beauty. Add to it the stupendous growth in literacy and the overall growth in human development, Kerala has rightly earned the sobriquet: “God’s own country”.
对任何游客来说,印度南部的喀拉拉邦都是一个有吸引力的目的地。热带气候和独特的回水系统为其原始之美增添了魅力。加上识字率的惊人增长和人类发展的整体增长,喀拉拉邦理所当然地赢得了“上帝自己的国家”的绰号。
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引用次数: 0
WELCOME BY CHAIRMAN TEA BOARD 茶会主席欢迎
Pub Date : 2004-08-01 DOI: 10.20425/IJTS.V3I3AND4.4610
Nisith Kr Das
Mr. N.K. Das was born in Assam on December 15, 1952. He studied at St. Edmunds College Shillong, graduating from Guwahati University. Subsequently, he did post graduation in history from Delhi University and obtained M.Sc. in Development Studies from the University of Bath, U.K.After joining the Indian Administrative Service in 1976, Mr. Das served in the states of Assam and Meghalaya in various capacities, including Commissioner& Secretary Tourism Govt of Assam and Divisional Commissioner of Lower Assam. He was Regional Director of Central Silk Board. Mr. Das served as Chairman cum Managing Director of Assam Tea Corporation, before taking over as the Chairman Tea Board in July 2000
N.K. Das先生1952年12月15日出生于阿萨姆邦。他曾就读于西隆圣埃德蒙兹学院,毕业于古瓦哈蒂大学。随后,他在德里大学获得历史学硕士学位,并在英国巴斯大学获得发展研究硕士学位。1976年加入印度行政服务局后,他曾在阿萨姆邦和梅加拉亚邦担任各种职务,包括阿萨姆邦旅游政府专员兼秘书和下阿萨姆邦地区专员。曾任中央丝绸局区域主任。他曾担任Assam Tea Corporation的董事长兼董事总经理,2000年7月接任主席茶董事会
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引用次数: 0
NEEM AS AN EFFECTIVE BIOCONTROL AGENT FOR TEA PESTS 印楝作为茶叶害虫的有效生物防治剂
Pub Date : 2004-03-01 DOI: 10.20425/IJTS.V3I1AND2.4603
S. Ramarethinam, S. Marimuthu, N. Murugesan
Plucking average, thereby, cost of harvesting of tea cannot be viewed in isolation as they are a product of multiple factors such as 'jat', age from pruning, height of bush and plucker, harvesting intervals and nutritional and health status of the bush. Use of mechanical implements, either hand-operated or motorized, if motorized, either held by one or two-men or self-propelled, contribute significantly to cost reduction in harvesting. However, choice of an appropriate implement to suit to the prevailing conditions in a region should be made carefully, lest the mechanization result in crop depression. Hand operated shears are currently in wide use without detriment to quality. The results with motorized, one or two-men held machines are varying, often leading to depression in quality and yield. They are in use in Japan, Russia, Argentina, Australia, Indonesia and Taiwan, but, in the importing countries they are proving uneconomic because of their high price and cost of maintenance; there is, thus, a need to develop machine(s) indigenously. The mounted, self-propelled machines are of limited application because of constraints in their manoeuvrability on gradients over 5°.
因此,采摘的平均成本不能孤立地看待,因为它们是多种因素的产物,如'jat',修剪的年龄,灌木和采摘器的高度,收获间隔以及灌木的营养和健康状况。使用机械工具,无论是手动的还是机动的,如果是机动的,由一个或两个人操作或自行驱动,大大有助于降低收割成本。但是,在选择适当的农具时应慎重考虑,以免机械化造成作物歉收。手动剪目前广泛使用,但不影响质量。一个人或两个人操作的电动机器的结果是不同的,经常导致质量和产量的下降。它们在日本、俄罗斯、阿根廷、澳大利亚、印度尼西亚和台湾使用,但在进口国,由于价格和维护成本高,它们被证明是不经济的;因此,有必要自主开发机器。由于其在坡度超过5°时的机动性受到限制,安装的自走式机器的应用受到限制。
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引用次数: 2
Tea as Nutraceutical 茶作为营养品
Pub Date : 2003-03-06 DOI: 10.20425/IJTS.V2I1AND2.4590
P. Pushpangadan, P. Latha
A strong link exists between health and food or drinks we consume. Many ailments like cancer, liver disorders, and diabetese etc. can be prevented and better health can be maintained by taking the right food or/and drinks. Such disease preventive and health protective food and drinks are called nutraceuticals. Tea contains powerful antioxidents like polyphenols and catechins and, therefore, has disease preventive, health protective and invigorating properties. Some scientific work carried out on the therapeutic and nutraceutic properties of tea are described in the present communication.
健康与我们消费的食物或饮料之间存在着密切的联系。许多疾病,如癌症、肝脏疾病和糖尿病等,都可以通过正确的饮食来预防和保持健康。这种预防疾病和保健的食品和饮料被称为营养保健品。茶含有强大的抗氧化剂,如多酚和儿茶素,因此,具有预防疾病,保护健康和提神的特性。在本通讯中描述了一些关于茶的治疗和营养特性的科学工作。
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引用次数: 0
Tea is a health-promoting beverage in lowering the risk of premature killing chronic diseases: a review. 茶是一种促进健康的饮料,可以降低过早死亡慢性病的风险:综述。
Pub Date : 2003-03-01 DOI: 10.1079/9781845931124.0001
J. Weisburger, N. Jain, M. Siddiqi
Green and black tea are widely used beverages, second only to water. Tea is an extract of the leaf of the plant Camellia sinensis . The major health benefit of tea is that the leaf contains several polyphenols such as epigallocatechin gallate, and in addition an enzyme, polyphenol oxidase. If upon harvest the leaves are heated, these enzymes are deactivated, and thus, after drying and grinding, the result is green tea. If the leaves are ground and incubated in air at about 40 degree C the polyphenol oxidase converts the original polyphenol to a number of other products such as theaflavin and thearubigins. Upon drying, these are the polyphenols typical of black tea. In most instances, the polyphenols from green tea and black tea have similar properties in health promotion. Coronary heart disease stems from the oxidation by reactive oxygen species of LDL-cholesterol. The tea polyphenols inhibit this reaction. There are data in humans that tea drinkers have a lower risk of heart disease. In animal models, similar findings were made. In the field of cancer causation, we distinguish between genotoxic carcinogens effecting DNA and genes, and other steps associated with the development of cancer, in part also involving reactive oxygen species. Tea and tea polyphenols induce enzymes such as glucuronosyl transferase that detoxify carcinogens. Thus, tea drinkers have a lower risk of cancer and the mechanisms of these reactions have been explored in animal models and through in vitro approaches. Of importance also is that tea decreases the growth of neoplastic cells, but not of normal cells. Tea also enhances apoptosis, a phenomenon of elimination of cancer cells. Tea drinkers also have a healthier intestinal flora, through the inhibition of bacteria that have adverse effects and promotion of the growth of beneficial bacteria. Through the elimination of reactive oxygen species, associated with premature aging, tea drinkers display good health to an old age.
绿茶和红茶是被广泛使用的饮料,仅次于水。茶是一种植物茶树叶子的提取物。茶的主要健康益处是茶叶含有几种多酚,如表没食子儿茶素没食子酸酯,此外还有一种酶,多酚氧化酶。如果在收获时加热叶子,这些酶就会失活,因此,在干燥和研磨之后,结果就是绿茶。如果茶叶被磨碎并在40摄氏度左右的空气中培养,多酚氧化酶将原来的多酚转化为许多其他产品,如茶黄素和茶红素。干燥后,这些是红茶中典型的多酚。在大多数情况下,绿茶和红茶中的多酚在促进健康方面具有相似的特性。冠心病是由低密度脂蛋白胆固醇的活性氧氧化引起的。茶多酚抑制了这一反应。有数据表明,喝茶的人患心脏病的风险较低。在动物模型中,也有类似的发现。在癌症病因领域,我们区分影响DNA和基因的基因毒性致癌物,以及与癌症发展相关的其他步骤,部分也涉及活性氧。茶和茶多酚能诱导葡萄糖醛酸转移酶等解毒致癌物的酶。因此,喝茶的人患癌症的风险较低,这些反应的机制已经在动物模型和体外方法中进行了探索。同样重要的是,茶可以减少肿瘤细胞的生长,而不是正常细胞的生长。茶还能促进细胞凋亡,这是一种消除癌细胞的现象。喝茶的人也有一个更健康的肠道菌群,通过抑制细菌的不利影响和促进有益菌的生长。通过消除与早衰有关的活性氧,喝茶的人可以健康到老年。
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引用次数: 8
期刊
茶叶科学
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