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Bio-efficacy of Certain Indigenous Plant Extracts against Red Spider Mite, Oligonychus coffeae , Nietner (Tetranychidae: Acarina) Infesting Tea 某些本土植物提取物对茶叶红蜘蛛螨、咖啡少爪螨、叶螨的生物功效研究
Pub Date : 2017-04-28 DOI: 10.5376/jtsr.2017.07.0004
B. Deka, A. Babu, M. Sarmah
Leaf extracts of five different locally available plants such as Polygonum hydropiper  L. , Vitex negundo L , Tithonia diversifolia (Hemsl.) (wild sun flower), Artemisia vulgaris L and Clerodendrum infortunatum L were evaluated for their ovicidal and acaricidal activity against the tea red spider mite, Oligonychus coffeae , Nietner (Tetranychidae: Acarina) in the laboratory using the leaf disc method under controlled conditions. Leaves collected from the above mentioned plants were dried under shade, milled in an electric blender and extracted using two different solvents, hexane and methanol and prepared different concentrations viz. 1%, 0.75%, 0.50% and 0.25% by diluting in DMSO (dimethyl sulfoxide; an important polar solvent) and were used for different bioassays. The effects of the extracts on the mortality of adults as well as in the eggs were assessed after spraying and the results were compared with an untreated control. All tested plants showed varying levels of efficacy on red spider mite compared when with the untreated control. Among five plant species tested, methanol extract of Vitex negundo and Clerodendrum infortunatum showed maximum ovicidal action (66.71% and 57.45% respectively) and exhibited more than 90% adult mortality @ 1% conc. of both the hexane and methanol extract of Vitex negundo and Clerodendrum infortunatum after 24 h.
在室内对照条件下,采用叶盘法测定了五种不同当地植物水蓼、牡荆花、野生向日葵、寻常蒿和金盏花叶提取物对茶红蜘蛛螨、咖啡少爪螨和叶螨的杀卵和杀螨活性。将上述植物的叶片遮阳干燥,在电动搅拌器中研磨,用己烷和甲醇两种不同的溶剂提取,用二甲基亚砜(DMSO)稀释,得到1%、0.75%、0.50%和0.25%的不同浓度;一种重要的极性溶剂),并用于不同的生物测定。在喷洒后评估了提取物对成虫和虫卵死亡率的影响,并将结果与未处理的对照进行了比较。与未经处理的对照相比,所有被试植物对红蜘蛛螨的功效都有不同程度的变化。在5种被试植物中,黄荆和黄竹甲醇提取物的杀卵作用最大(分别为66.71%和57.45%),在1%浓度下成虫死亡率超过90%。黄荆和黄荆的己烷和甲醇提取物在24 h后的作用。
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引用次数: 9
Effect of Tea on Gingivits: A Community-based Study 茶对牙龈的影响:一项社区研究
Pub Date : 2017-04-20 DOI: 10.5376/jtsr.2017.07.0005
A. Laskar, C. Paul, B. Laskar
Pharmacological values of tea have been found by medical researchers in many countries. Catechin compounds found in tea impart several health benefits including mitigating dental ailments. However, whether dento-clinical properties of catechins are camouflaged by additive like milk or not has remained unexplored. A survey-based study involving community people suffering from gum disease (gingivitis) has been carried out to understand whether hot water extract of tea ( known as brew) without milk has any relevance in reducing gingivitis. The survey reveals a strong negative linear correlation ( r= -0.807** ) between drinking tea without milk and reduction of gingivitis. The results also reveal that frequency of gingivitis amongst people who drink tea with milk is more ( r= 0.696* ) as compared to the people who drink tea without milk. Tea made from Tinali 17/1/54 cultivar of Camelia sinensis L. O. Kuntze var, Assamica has been used in the study as this cultivar is ubiquitous in tea growing areas and can produce different categories of black tea – strong CTC tea, mild flavored orthodox tea and milder green tea.
茶的药理价值已被许多国家的医学研究者发现。茶中发现的儿茶素化合物具有多种健康益处,包括减轻牙齿疾病。然而,儿茶素的牙齿-临床特性是否被添加剂如牛奶所掩盖仍未被研究。一项基于调查的研究涉及患有牙龈疾病(牙龈炎)的社区人群,以了解不加牛奶的热水茶提取物(称为冲泡茶)是否与减少牙龈炎有关。调查显示,喝茶不加牛奶与减少牙龈炎之间存在很强的负线性相关(r= -0.807**)。结果还显示,与不喝牛奶的人相比,喝牛奶茶的人患牙龈炎的频率更高(r= 0.696*)。由于该品种在茶叶种植区普遍存在,可以生产出不同种类的红茶——浓郁的CTC茶、温和的正统茶和温和的绿茶,因此研究中使用的是Tinali 17/1/54茶树品种Kuntze var, Assamica。
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引用次数: 0
Effects of Micronutrients on the Nutritional Status of Clonal Tea Replanted in Areas Where Old Tea Was Uprooted 微量元素对老茶连根拔地再植无性系茶营养状况的影响
Pub Date : 2017-03-20 DOI: 10.5376/jtsr.2017.07.0003
W. Omwoyo, Olunga Sween, S. Kibet, F. Oloo
Tea yields peak is 21-30 years after which yields decline. The declining productivity and moribuncy has been reported in many tea fields due to prolonged period of monoculture under tea, physicochemical and biological properties of soil deteriorate considerably causing diseases, pests, acidic soils among others which leads to uprooting of old tea bushes. Deficiency of micronutrients in some tea sections has been reported which has been corrected by foliar application. Though the deficiency could be corrected through foliar application, this mode of application has its shortcomings including leaf burn and the mobility of nutrients from the leaves to the roots is very slow. This study aimed at determining the effect of soil application and variation of micronutrients on the nutritional status of clonal tea replanted in old tea lands.Leaf and soil samples were collected in a random complete block design and the micromutrient levels determined using Inductively Coupled Plasma Emission (ICPE). Data analysis was done using MSTAT-C software.The changes in the levels of micronutrients with varied eight micronutrient combinations subjected on different clones were studied. Clone 12/28 significantly (P≤0.05) varied with the other clones indicating that different clones have varied abilities to absorb nutrients. Clone 303/577 gave a significantly higher uptake of B in the order TRFK303/577 >S15/10 >12/28 >31/8. Clone 12/28 recorded a higher uptake of N with mean levels of 3.075, clone 31/8 had significantly higher uptakes of Fe and Zn. None of the micronutrients subjected on the clones brought an impact in the uptake of N and Mg. This study therefore recommends the use of soil application of micronutrients as an alternative to foliar application in areas where old tea bushes were uprooted.
茶叶产量在21-30年达到顶峰,之后产量下降。据报道,由于长期单茶树栽培,许多茶园的生产力下降,枯死,土壤理化和生物特性严重恶化,引起病虫害,土壤酸化等,导致老茶树连根拔起。据报道,一些茶种的微量营养素缺乏,已通过叶面施用得到纠正。虽然叶面施用可以弥补这一缺陷,但这种施用方式也有其缺点,包括叶片烧伤和营养物质从叶片到根系的流动速度很慢。本研究旨在探讨土壤施用和微量元素变化对老茶园再植无性系茶营养状况的影响。采用完全随机区组设计采集叶片和土壤样品,采用电感耦合等离子体发射(ICPE)测定微量元素水平。数据分析采用MSTAT-C软件。研究了8种不同微量元素组合在不同无性系上的微量元素含量变化。无性系12/28与其他无性系差异显著(P≤0.05),说明不同无性系对养分的吸收能力不同。克隆303/577对B的吸收量显著高于TRFK303/577 >S15/10 >12/28 >31/8。无性系12/28对氮的平均吸收量为3.075,无性系31/8对铁和锌的吸收量显著高于无性系31/8。施用于无性系的微量元素对氮和镁的吸收均无影响。因此,本研究建议在老茶树被连根拔起的地区,使用土壤施用微量营养素作为叶面施用的替代方法。
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引用次数: 0
Practical Utilization of Botanical Extracts and Microbial in Controlling Dieback Disease of Tea [ Camellia sinensis (L) O. Kuntze] Caused by Fusarium solani (Mart.) Sacc. 植物提取物和微生物在防治枯萎病中的应用Sacc。
Pub Date : 2017-03-03 DOI: 10.5376/JTSR.2017.07.0002
S. R. Sarmah, P. K. Baruah, S. Das
In Northeast Indian tea plantations infection of Fusaruim solani , resulting dieback of tea plant is increasing which causes considerable crop loss during the recent times. In this investigation, native plant extracts, i.e. Acorus calamus L., Azadirachta indica A. Juss., Clerodendrum viscosum Vent., and Xanthium strumarium L. and microbials i.e. Bacillus subtilis and Trichoderma viride Pers. were utilized to evaluate the efficacy in controlling dieback disease of tea. These extracts inhibited the growth of Fusarium solani by 60-90%. In field application of C. viscosum and X. strumarium extracts reduced the disease up to 89.3% and 81% respectively. More than 70% disease reduction was observed when aqueous extracts of A. calamus and A. indica were used separately. Maximum disease reduction was achieved up to 86.9% due to application of T. viride . The results, thus, suggested the potential use of herbal extracts and microbial strains as an effective component of integrated disease management (IDM) schedule in the organic tea farming. The methods and rate of application are also discussed.
近年来,印度东北部茶园因枯萎病的发生,导致茶树枯死现象日益严重,造成相当大的作物损失。本研究以菖蒲、印楝等天然植物提取物为研究对象。,粘枝胶孔。等微生物,如枯草芽孢杆菌和绿色木霉。利用不同药剂对茶叶枯死病的防治效果进行了评价。这些提取物对茄枯菌生长的抑制作用为60 ~ 90%。田间应用粘草提取物和鹤耳草提取物,防治效果分别为89.3%和81%。菖蒲水提液与籼稻水提液分别使用时,病害降低率达70%以上。施用绿t菌可最大限度地降低病死率达86.9%。因此,草药提取物和微生物菌株有可能作为有机茶园病害综合管理(IDM)计划的有效组成部分。讨论了应用的方法和速度。
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引用次数: 3
Effects of Nitrogenous Fertilizer Rates, Plucking Intervals and Geographical Location of Production on Selected Micronutrient Levels of the Black Tea 氮肥用量、采采间隔和生产地理位置对红茶微量元素选择水平的影响
Pub Date : 2017-02-06 DOI: 10.5376/JTSR.2017.07.0001
W. Omwoyo
Micronutrients are important to both the human life and the tea plant. Camellia sinensis is widely grown in East Africa and its beverages are claimed to be the most widely consumed fluids after water. The changes in the levels of micronutrients with varied rates of nitrogenous fertilizer and plucking intervals three different locations were studied. All the studied micronutrients significantly (p≤0.05) varied with location of production. Mn and Se levels did not significantly (p≤0.05) change with an increase in nitrogenous fertilizer rates. Fe and Zn significantly (p≤0.05) increased with an increase in nitrogen fertilizer rates while Cu significantly (p≤0.05). The micronutrient content of the black teas did not significantly (p≤0.05) change with varied plucking intervals. There is need to strike a balance in the application of nitrogenous fertilizer rates in different locations in order to harmonize all the micronutrients. This research recommends 150 kg/ha/year of nitrogenous fertilizer rate that will strike a balance on all the studied micronutrients.
微量元素对人类和茶树都很重要。茶树在东非广泛种植,其饮料据称是仅次于水的最广泛消费的液体。研究了不同氮肥施用量和采采间隔对3个不同地点微量元素含量的影响。各微量元素含量随产地差异显著(p≤0.05)。Mn、Se水平随氮肥施用量的增加无显著变化(p≤0.05)。随着氮肥施用量的增加,铁和锌显著升高(p≤0.05),铜显著升高(p≤0.05)。不同采期红茶中微量元素含量变化不显著(p≤0.05)。为了使所有微量元素协调一致,需要在不同地点的氮肥施用中取得平衡。本研究建议施用150公斤/公顷/年的氮肥,使所研究的所有微量元素达到平衡。
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引用次数: 0
Enhanced Acaricidal Activity of Senna auriculata and Ocimum tenuiflorum Extracts with the Addition of Adjuvant against Red Spider Mite, Oligonychus coffeae Nietner (Acarina: Tetranychidae) Infesting Tea 添加佐剂增强木耳番泻叶和花竹提取物对茶叶红蜘蛛螨、咖啡少爪螨的杀螨活性
Pub Date : 2016-12-27 DOI: 10.5376/JTSR.2016.06.0010
Pandian Kumar, C. Sundaravadivelan, D. V. Kumar, A. Babu
Tea ( Camellia sinensis ) is one of the most consumed non-alcoholic beverages in the world and has been affected by many pests which damage its quality and production globally. In this paper an attempt has been made to evaluate the aqueous leaf extracts of Senna auriculata and Ocimum tenuiflorum with and without adjuvant for their effect on red spider mite (RSM), Oligonychus coffeae (Nietner) infesting tea. Resulting, Senna auriculata, leaf extracts with adjuvant caused 90 % (LC 50 :1.21 and LC 90 :2.33 %) mortality which was greater compared to Ocimum tenuiflorum whereas 73 % (LC 50 :1.40 and LC 90 :2.81 %) was observed at 5 % concentration after 24 h exposure and very lowest mortality percent were observed both extracts without adjuvant. Ocimum tenuiflorum leaf extracts with adjuvant (0.05 %) showed highest ovicidal activity and effective ovipositional deterrence compared with S. auriculata treatment. The evaluated plant extracts with adjuvant are effective for controlling RSM and it could be an alternative, easily accessible, and eco-friendly approach for control of mite infesting tea.
茶(Camellia sinensis)是世界上消费量最大的非酒精饮料之一,受到许多害虫的影响,影响其质量和全球产量。本文研究了木耳泻泻叶和竹叶泻泻叶的水提液在加和不加佐剂的情况下对茶叶中红蜘蛛螨、咖啡Oligonychus的防治效果。结果表明,加佐剂的木耳泻叶提取物在24 h后的死亡率为90% (lc50:1.21和lc90: 2.33%),高于浓度为5%时的木耳泻叶提取物的死亡率为73% (lc50:1.40和lc90: 2.81%),未加佐剂的木耳泻叶提取物的死亡率最低。与木耳草处理相比,加佐剂(0.05%)的芦竹叶提取物的杀卵活性和产卵抑制效果最高。所评价的植物提取物加佐剂对茶螨有较好的防治效果,是一种简便、环保的防治茶螨的新方法。
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引用次数: 0
Laboratory and Field Evaluation of an Entomopathogenic Fungus Formulation-Bioterminator (Metarhizium Anisopliae Metchnikoff) against Termite Infesting Tea 金龟子绿僵菌(Metarhizium Anisopliae Metchnikoff)拮抗茶叶白蚁的室内及现场评价
Pub Date : 2016-12-22 DOI: 10.5376/JTSR.2016.06.0009
Hoque A.K.M.R., Aslam A.F.M., M. Ahmed, Mamun M.S.A., A. Howlader
Experiments were carried out to determine bioefficacy of an entomopathogenic fungus formulation - Bioterminator ( Metarhizium anisopliae ) against termites infesting tea during February, 2015 to October 2015. The experiment was performed in both laboratory and the field conditions. In the laboratory experiment, mortality data were recorded daily up to 7 days after treatment (DAT) and at monthly interval for field experiments. Result revealed from the laboratory bioassay that Bio-terminator was found to be effective (43.28-72.94%) up to 7 days after treatment. In field condition, Bio-Terminator was found to be highly effective (85.21, 84.91 & 85.97% respectively) in controlling termites and maintain their effectiveness to the desired level up to 9 months. Therefore, Bio-terminator may be efficiently used for the management of termites in tea as the component of Integrated Pest Management.
2015年2月至2015年10月,对一种昆虫病原真菌制剂-绿僵菌(Metarhizium anisopliae)对茶叶白蚁的生物杀灭效果进行了试验研究。试验在实验室和田间条件下进行。在室内试验中,每天记录死亡率数据,直至治疗后7天(DAT),并按月间隔进行现场试验。实验室生物测定结果显示,生物终结者在治疗后7天有效(43.28-72.94%)。在田间条件下,生物终结者对白蚁的防治效果为85.21%、84.91和85.97%,并可维持药效达9个月。因此,作为害虫综合治理的组成部分,生物杀灭剂可以有效地用于茶叶白蚁的治理。
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引用次数: 6
Wage determination machinery of tea industry in India: a case of West Bengal State 印度茶业的工资决定机制:以西孟加拉邦为例
Pub Date : 2016-10-24 DOI: 10.5376/jtsr.2016.06.0008
D. Biswas
Daily rate of wages for tea plantation workers in west Bengal is abysmally low in comparison to agricultural minimum wages. There are a few benefits offered by the employers apart from the statutory benefits enshrined in the Plantation Labour Act, 1951. Consequently, many tea workers in this state are suffering from starvation, malnutrition, debt, etc. In this backdrop, in my present study, a deliberate attempt has been made in order to highlight the actual picture of wage and its determination process in the tea industry located in West Bengal in India.
与农业最低工资相比,西孟加拉邦茶园工人的日工资低得可怜。除了1951年《种植园劳工法》规定的法定福利外,雇主还提供一些福利。因此,许多茶工在这个国家遭受饥饿,营养不良,债务等。在此背景下,在我目前的研究中,为了突出位于印度西孟加拉邦的茶业工资及其确定过程的实际情况,我进行了一次刻意的尝试。
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引用次数: 1
The odor of matcha (Japanese powdered green tea) as the base note of green tea leaves 抹茶(日本粉末状绿茶)的气味,作为绿茶的基调
Pub Date : 2016-04-15 DOI: 10.20425/IJTS.V0IOF.9577
Toshio Hasegawa, Yasutsugu Tsukumo, Takashi Fujita, T. Fujihara, A. Takahashi, K. Nakajima
We previously reported the group of aroma constituents that characterize the odor of sencha (green tea made from leaves) which is similar to but distinct from the odor of commercially available matcha (Japanese green tea made from powder).This study, investigated whether the group of aroma compounds contributing to this matcha-like odor is present in all green tea cultivars and products. Crude sencha teas made from the 'Yabukita', 'Sayamakaori', 'Samidori', and 'Ujimidori' cultivars, and teas prepared by different processes (hojicha and unkacha), were investigated. These green tea leaves had a common matcha-like odor originating from a group of identical constituents. Commercially available matcha were studied as the source of the standard odor. The extracts obtained from matcha were separated into groups with different odors by fractional distillation. Chemical and analytical methods showed that the group of key compounds producing the matcha-like odor included a minute quantity of several aliphatic aldehydes. Many aldehyde proton signals were observed by 1H NMR and some aldehydes were detected by mass spectroscopy. It was concluded that a matcha-like odor that contained some aliphatic aldehydes was the base note of green tea leaves, indicating the importance of the matcha-like odor to green tea's odor character.
我们之前报道了一组香气成分,这些成分表征了煎茶(由叶子制成的绿茶)的气味,它与市售的抹茶(由粉末制成的日本绿茶)的气味相似但不同。这项研究调查了所有绿茶品种和产品中是否存在导致这种抹茶样气味的香气化合物组。研究了由“Yabukita”,“Sayamakaori”,“Samidori”和“Ujimidori”品种制成的粗煎茶,以及通过不同工艺(hojicha和unkacha)制备的茶。这些绿茶叶子有一种类似抹茶的共同气味,来自一组相同的成分。研究了市售抹茶作为标准气味的来源。用分馏法将抹茶提取物分为不同气味组。化学和分析方法表明,产生抹茶样气味的关键化合物组包括少量的几种脂肪醛。用核磁共振氢谱法观察到许多醛质子信号,用质谱法检测到一些醛。结果表明,含有脂肪醛的抹茶类气味是绿茶的底调,说明抹茶类气味对绿茶的气味特性具有重要作用。
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引用次数: 3
Dissipation rates of some selected pesticides from the soil of tea agroecosystem in Assam, India 印度阿萨姆邦茶叶农业生态系统土壤中某些农药的耗散率
Pub Date : 2016-04-15 DOI: 10.20425/IJTS.V0IOF.9578
Yumnam Devashree, B. Dutta, S. Paul, Sudip Choudhury
An experiment was carried out in Rosekandy Tea Estate, Barak Valley, Assam to investigate the persistence of some selected pesticides (i.e Endosulfan, Fipronil, Paraquat and 2,4-D) on the soil. These pesticides were applied in the soil as per the application rate, where one year old tea plants were growing and the residue were analysed for a period of 50 days. Their degradation kinetics in soil was also studied and described. Soil samples were collected with the help of soil corer. The samples were brought to the laboratory, air dried in room temperature and extracted for analysis with high performance liquid chromatography (HPLC). Among the pesticides 2,4-D was highly persistent with half life of 57.75 days followed by Endosulfan which lost 42.64% of its initial concentration by the last sampling period (i.e 50 days) after the spraying. Fipronil and paraquat lost 65% and 70.32% of initial residue in soil making them the least persistent amongst these pesticides in the studied soil. Considering the impact of pesticide residues on human health, the less persistent pesticides may be used in tea plantations to minimize the potential negative impact of pesticides in the environment. The cost benefit ratios of the use of the pesticides should be also worked out.
在阿萨姆邦巴拉克山谷的罗斯坎迪茶园进行了一项试验,以调查一些选定的农药(即硫丹、氟虫腈、百草枯和2,4- d)在土壤上的持久性。这些农药按施药率施用于土壤中,在生长了一年的茶树的土壤中,对残留进行了为期50天的分析。研究并描述了它们在土壤中的降解动力学。利用土壤覆盖物采集土壤样品。样品被带到实验室,在室温下风干,提取用于高效液相色谱(HPLC)分析。其中,4-D的持久性较强,半衰期为57.75 d,其次是硫丹,在喷洒后最后一个采样期(即50 d),硫丹的半衰期下降了42.64%。氟虫腈和百草枯在土壤中损失了65%和70.32%的初始残留,使它们在所研究的土壤中持久性最低。考虑到农药残留对人体健康的影响,可以在茶园中使用持久性较低的农药,以尽量减少农药对环境的潜在负面影响。还应计算出使用农药的成本效益比。
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引用次数: 0
期刊
Journal of Tea Science
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