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INSECTICIDE TOXICITY TO HONEY BEES: LESSONS LEARNED FROM STUDIES BY THE UNIVERSITY OF FLORIDA URBAN ENTOMOLOGY LABORATORY 杀虫剂对蜜蜂的毒性:佛罗里达大学城市昆虫学实验室研究的经验教训
Pub Date : 2024-03-21 DOI: 10.32473/jfmca.71.1.135290
Rebecca Baldwin, Roberto M. Pereira, Phillip G. Koehler, Rui-De Xue
Laboratory and field studies comparing the relative tolerance of mosquitoes (Aedes aegypti and Aedes albopictus) and non-target species, including the honey bee (Apis mellifera), and other non-target insects have shown that the non-target species can tolerate fairly well when exposed to insecticides used in mosquito control operations. Tolerance levels of non-target species vary due to factors such as the development of insecticide resistance in mosquitoes which require higher insecticide doses to reduce populations to adequate levels. However, well-planned mosquito control programs, can lower risks to honey bees and other non-target species. This review discusses the toxicity of mosquito control insecticides to the honey bee and other non-target insects.
实验室和实地研究比较了蚊子(埃及伊蚊和白纹伊蚊)和非目标物种(包括蜜蜂(Apis mellifera)和其他非目标昆虫)的相对耐受性,结果表明,非目标物种在接触灭蚊作业中使用的杀虫剂时,耐受性相当好。由于蚊子对杀虫剂产生抗药性等因素,非目标物种的耐受水平各不相同,这就需要更高的杀虫剂剂量才能将蚊子数量减少到适当水平。不过,计划周密的灭蚊计划可以降低蜜蜂和其他非目标物种面临的风险。本综述讨论了灭蚊杀虫剂对蜜蜂和其他非目标昆虫的毒性。
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引用次数: 0
USING BG LURE VERSUS NON-ATTRACTANT IN STICKY TRAPS TO EVALUATE EFFECTIVENESS OF COLLECTING EYE GNATS (DIPTERA: CHLOROPIDAE: LIOHIPPELATES SPP.) 粘捕器中诱蚊剂与非诱蚊剂诱蚊效果比较(双翅目:蠓科:蠓科)
Pub Date : 2023-05-02 DOI: 10.32473/jfmca.70.1.133843
M. Pogue, R. Xue, Qing‐He Zhang
Increased number of eye gnats often cause a nuisance problem in St. Johns County, Florida, during spring and summer. Anastasia Mosquito Control District, St. Augustine often receives service requests and complaints about the eye gnat problem. Evaluation of traps is critically important for surveillance and control of nuisance eye gnats. This study was conducted to determine if sticky traps (RESCUE!® TrapStik for Flies) baited with BG Lure were more attractive to eye gnats than sticky traps without bait in Elkton, northeastern Florida. The results showed that sticky traps baited with BG Lure were more attractive to eye gnats than unbaited traps, which could be considered as a tool for surveillance and control of eye gnats.
在佛罗里达的圣约翰县,在春夏季节,眼蚊数量的增加常常会给人们带来麻烦。圣奥古斯丁的阿纳斯塔西娅蚊虫控制区经常收到有关蚊蚋问题的服务请求和投诉。对捕蚊器的评估对监测和控制有害蚊蚋至关重要。本研究是为了确定粘捕器(RESCUE!在佛罗里达州东北部的埃尔克顿,用BG诱捕剂诱捕眼蚊比没有诱捕剂的粘性诱捕器更有吸引力。结果表明,布袋诱蚊饵黏性诱蚊器对眼小蚊的诱蚊效果优于无饵诱蚊器,可作为眼小蚊监测和控制的一种手段。
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引用次数: 0
EFFECTIVENESS OF A COMMERCIAL COMPETITIVE ELISA FOR THE DETECTION OF WEST NILE VIRUS ANTIBODIES IN SENTINEL CHICKENS 商业竞争性elisa法检测哨鸡西尼罗病毒抗体的有效性
Pub Date : 2023-05-02 DOI: 10.32473/jfmca.70.1.133968
S. Peper, Milton Sterling
The use of sentinel chickens is a powerful tool to aid in arboviral surveillance efforts. Results from this type of surveillance is used to help guide abatement efforts for vector species. Many mosquito control programs use outsourced laboratories for testing sentinel chicken samples. This study evaluated the use of a commercially available competitive enzyme-linked immunosorbent assay (cELISA) for in-house testing for West Nile virus antibodies from chicken serum. The commercial cELISA detected more positive sentinel chickens compared to the outsourced laboratory results. Results obtained from the in-house testing of chicken serum were reported sooner compared to the outsourced laboratory results, enhancing mosquito control programs timeliness for response to mosquito-borne disease.
哨兵鸡的使用是帮助虫媒病毒监测工作的有力工具。这类监测的结果用于帮助指导减少病媒物种的工作。许多蚊子控制项目使用外包实验室检测哨鸡样本。本研究评估了市售竞争性酶联免疫吸附测定法(cELISA)在鸡血清中西尼罗病毒抗体内部检测中的应用。与外包实验室结果相比,商业cELISA检测到更多阳性哨兵鸡。与外包实验室结果相比,报告鸡血清内部检测结果的时间更早,从而提高了蚊虫控制规划应对蚊媒疾病的及时性。
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引用次数: 0
EVALUATION ON THE ACTIVITY AND EFFICACY OF OMNIPRENE 20CS AGAINST AEDES, ANOPHELES AND CULEX MOSQUITOES IN OUTDOOR MICROCOSMS 全蚊20cs对室外微环境中伊蚊、按蚊和库蚊的杀灭效果评价
Pub Date : 2023-05-02 DOI: 10.32473/jfmca.70.1.133687
T. Su, H. Su
Mosquito control by pesticides remains the main intervention to reduce the burden of mosquito nuisance and mosquito-borne diseases. In mosquito control, larviciding by application of environmentally friendly larvicides has been one of the routine operations. Larvicides based on microbial agents and insect growth regulators (IGRs) account for most of the available products. Ones that are formulated using S-methoprene ranged from microencapsulated concentrate (or capsule suspension - CS), various granules, and pellets to briquets for different habitats. The CS formulation has been among the traditional products based on S-methoprene due to its advantages of easy application, fast action, and even area coverage. The current paper evaluated a newly developed CS formulation OmniPrene 20CSTM containing 20% S-methoprene against mosquitoes of public health importance. High inhibition of emergence activity was indicated against the test species, and Aedes and Anopheles mosquitoes showed higher susceptibility than Culex in laboratory bioassays. The performance under field conditions exceeded the label specification of the currently available products with the same application pattern. Over the 21-day evaluation period, the control levels ranged 86.4-100%, 89.8-100% and 79.8-100% against Aedes aegypti, Anopheles hermsi and Culex quinquefasciatus respectively when it was applied at the intended label dose of 54.8-73.1 mL/ha. This product is expected to be used as one of the viable tools along with other available products to combat mosquitoes of public health concerns.
用除害剂灭蚊仍然是减少蚊虫滋扰和蚊媒疾病负担的主要干预措施。在灭蚊工作中,使用环境友好型杀幼虫剂灭蚊已成为常规工作之一。以微生物剂和昆虫生长调节剂(IGRs)为基础的杀幼虫剂占可用产品的大部分。使用s -甲基戊二烯配制的药物包括微胶囊浓缩物(或胶囊悬浮液- CS)、各种颗粒、颗粒和砖状颗粒,适用于不同的栖息地。CS制剂具有应用方便、见效快、覆盖面积均匀等优点,是基于s -甲氧二烯的传统产品之一。本文评价了新开发的含有20% s -甲氧二烯的抗蚊制剂OmniPrene 20CSTM对具有公共卫生重要性的蚊子的作用。实验结果表明,该药剂对被试蚊的羽化活性具有较高的抑制作用,伊蚊和按蚊的敏感性高于库蚊。在现场条件下的性能超过了相同应用模式下现有产品的标签规格。21 d评价期内,在54.8 ~ 73.1 mL/ha的标示剂量下,对埃及伊蚊、荷氏按蚊和致倦库蚊的控制水平分别为86.4 ~ 100%、89.8 ~ 100%和79.8 ~ 100%。预计该产品将与其他现有产品一起作为可行的工具之一,用于防治引起公共卫生关注的蚊子。
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引用次数: 1
rise and fall of St. Louis Encephalitis Virus in Florida 佛罗里达圣路易斯脑炎病毒的兴衰
Pub Date : 2023-05-02 DOI: 10.32473/jfmca.70.1.133685
John V. Day
St. Louis encephalitis virus has had a fascinating history in Florida. The virus was introduced into the Miami area in the early 1950s. This introduction resulted in two human outbreaks, one in 1952 and a second in 1958. Three urban SLE epidemics were reported in the Tampa Bay area in 1959, 1961, and 1962. The virus virtually disappeared from the state until 1977 when a widespread rural SLE epidemic was reported in 20 Florida counties with 110 confirmed human cases. An almost identical rural SLE epidemic was reported in 1990 when 226 human SLE cases were reported in 28 Florida counties. Following the introduction of West Nile virus into Florida in 2001, reports of SLEV transmission to sentinel chickens and humans decreased dramatically. Except for a 2014 focal outbreak of two SLE cases in Duval County, only sporadic transmission of SLEV to humans and sentinel chickens was reported in the state between 1998 and 2022.
圣路易斯脑炎病毒在佛罗里达州有一段迷人的历史。该病毒于20世纪50年代初传入迈阿密地区。这导致了两次人间暴发,一次在1952年,第二次在1958年。1959年、1961年和1962年在坦帕湾地区报告了三次城市SLE流行。直到1977年,该病毒才从该州消失,当时在佛罗里达州20个县报告了广泛的系统性红斑狼疮农村流行病,并确诊了110例人类病例。1990年,在佛罗里达州28个县报告了226例人类SLE病例,报告了几乎相同的农村SLE流行。继2001年西尼罗河病毒传入佛罗里达州后,哨岗鸡和人类感染SLEV的报告急剧减少。除了2014年杜瓦尔县两例SLE病例的焦点暴发外,1998年至2022年期间,该州仅报告了SLEV向人类和哨岗鸡的散发传播。
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引用次数: 0
LABORATORY EXPLORATION OF TOLFENPYRAD AND NATURECIDE IN TOXIC SUGAR BAITS AGAINST AEDES AEGYPTI 苯氰吡虫啉和天然杀菌剂对埃及伊蚊毒糖饵的实验室研究
Pub Date : 2023-05-02 DOI: 10.32473/jfmca.70.1.133841
Dillon Streuber, C. Bibbs, G. Muller, R. Xue
Attractive toxic sugar baits (ATSB) are an effective mosquito control tool based on sugar feeding behaviors and oral ingestion. There is a demand from consumers for more effective active ingredients. Torac 15EC, containing tolfenpyrad, is a registered insecticide for control of agricultural pests, which may mean transferability to mosquito control. Another option, Naturecide Pest Management (NPM), is a botanical insecticide containing cedarwood and cinnamon oils for control of adult mosquitoes via contact. This study evaluated tolfenpyrad and a formulated essential oil blend added to toxic sugar baits (TSB) against adult Aedes aegypti, compared with a positive control of 1% boric acid toxic sugar bait, and untreated control. In this experiment setup, ingestions of tolfenpyrad at 1%, NPM at 1% (of finished product) TSB, and boric acid at 1% by female Ae. aegypti mosquitoes resulted in average mortality at 48 hrs were 71%, 73% and 95%, respectively. The results suggest that ingestible tolfenpyrad, Naturecide Pest Management, and boric acid TSBs may be potential tool for mosquito control strategies and programs, but the mode of action of essential oils to kill adult mosquitoes is still needed to be addressed.
诱食糖饵是一种基于食糖摄食行为和口服摄食的有效蚊虫控制手段。消费者对更有效的活性成分有需求。Torac 15EC是一种已注册的防治农业害虫的杀虫剂,含有苯虫吡酯,这可能意味着它可以转移到蚊虫防治上。另一种选择是自然杀虫管理(NPM),它是一种植物性杀虫剂,含有雪松木和肉桂油,通过接触控制成年蚊子。本研究评估了添加到有毒糖饵(TSB)中的苯虫吡酯和一种配方精油混合物对埃及伊蚊成虫的作用,并与1%硼酸有毒糖饵的阳性对照和未经处理的对照进行了比较。在本实验设置中,雌性伊蚊摄入1%的甲苯吡虫胺、1%的NPM(成品)TSB和1%的硼酸。48小时时埃及伊蚊平均死亡率分别为71%、73%和95%。结果提示,可摄取性灭蚊菊酯、自然杀虫剂和硼酸TSBs可能是蚊虫控制策略和规划的潜在工具,但精油对成蚊的作用方式仍有待研究。
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引用次数: 0
AN ASSESSMENT OF A LETHAL OVIPOSITION TRAP’S ABILITY TO PRODUCE IMMATURES OF AEDES ALBOPICTUS IN PANAMA CITY BEACH, FLORIDA 在佛罗里达州巴拿马城海滩,一种致命的产卵诱捕器产生白纹伊蚊的能力评估
Pub Date : 2023-05-02 DOI: 10.32473/jfmca.70.1.133692
Michael T. Riles, Kaylyn Cullen, Mark Clifton, James Clauson
A series of tests were performed in the laboratory and in the field, 2014-2017, assessing the efficacy of the Trap ‘N’ Kill (TNK) (Springstar® Inc., Woodinville, WA) lethal ovitrap on Aedes albopictus adults and immatures. The TNK lethal ovitrap design is to attract and kill adult female mosquitoes as well as any larvae that emerge over a claimed 45-day period by utilizing the active ingredient dichlorvos. Cage trials demonstrate that the trap effectively killed wild-caught, adult mosquitoes (> 80% mortality) for up to 4 weeks (28 days). After 4 weeks, the ability of the trap to control adult mosquitoes declined reaching only 18.8 % mortality by week 7. A field trial demonstrated a similar 4-week maximum effective duration of the TNK trap when immature mosquitoes had emerged from eggs and were present in all traps after 4 weeks (6 weeks, 15.2 ± 2.56 % larvae per trap were observed). A field trial was also conducted to assess the ability of the TNK trap to reduce adult mosquito populations over a ~5-acre area in a residential neighborhood. No reduction in adult mosquito population could be detected with the trap density (5 traps/acre) and experimental conditions utilized in this trial.
2014-2017年,在实验室和现场进行了一系列试验,评估了Trap ' N ' Kill (TNK) (Springstar®Inc., Woodinville, WA)致死性诱卵器对白纹伊蚊成虫和幼虫的效果。秋明致死性诱蚊器设计是利用有效成分敌敌畏,吸引并杀死在声称的45天内出现的成年雌蚊和任何幼虫。笼试验表明,该诱捕器可有效杀灭野生成蚊(死亡率> 80%)长达4周(28天)。4周后,诱蚊器对成蚊的控制能力下降,第7周死亡率仅为18.8%。现场试验表明,当未成熟的蚊子从卵中出来并在4周后出现在所有陷阱中时,TNK陷阱的最长有效时间为4周(6周,观察到每个陷阱的幼虫为15.2±2.56%)。还进行了实地试验,以评估TNK诱捕器在居民区约5英亩区域内减少成蚊种群的能力。采用诱捕器密度(5个/亩)和试验条件均未发现成蚊数量减少。
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引用次数: 0
FIELD EVALUATION OF LURES AS CANDIDATE ATTRACTANTS FOR COASTAL CULICOIDES IN FLORIDA 诱蚊饵作为佛罗里达州沿海库蠓候选引诱剂的现场评价
Pub Date : 2023-05-02 DOI: 10.32473/jfmca.70.1.133690
A. Lloyd, D. Kline, D. Hahn
Biting midges (Diptera: Ceratopogonidae: Culicoides) along the coast of Florida are a severe biting nuisance that can impede outdoor activities. Methods currently available for biting midge control are limited due to environmentally sensitive larval habitats and the lack of adulticide techniques available for area-wide population suppression.Homeowners that live along the coast can protect their homes with the use of fine- meshed window screens and fans, coarse low volume adulticide applications, and adult removal via commercial mosquito traps. There are known attractants that have been used to enhance trap captures for adult mosquitoes, and it is logical to test these attractants against closely related adult hematophagous Diptera such as Culicoides. This study compared four attractant lures, octenol, BG lure, R-octenol, and USDA red blend, known to be attractive to mosquitoes, and tested them against C. furens and C. mississippiensis as an addition to increase trap capture. Although all attractants increased trap capture above the control, results from this study could not identify a lure that was significantly more attractive to Culicoides species captured in Cedar Key, Florida. Further studies evaluating species specific trap types as well as attractants are needed to develop an effective Culicoides adult trapping control system as part of an integrated biting insect control program.
沿佛罗里达海岸的蠓(双翅目:蠓科:库蠓)是一种严重的叮咬滋扰,可以妨碍户外活动。由于对环境敏感的幼虫栖息地和缺乏可用于全区域种群抑制的杀虫技术,目前可用于控制蠓的方法有限。居住在沿海地区的房主可以通过使用细孔窗纱和风扇,少量使用杀虫剂,以及通过商业捕蚊器清除成虫来保护他们的家园。有一些已知的引诱剂已被用于加强对成蚊的诱捕,因此将这些引诱剂用于与成蚊密切相关的吸血双翅目昆虫,如库蠓,是合乎逻辑的。本研究比较了辛烯醇、BG引诱剂、r -辛烯醇和USDA红色混合引诱剂这4种诱蚊剂对蚊子的引诱作用,并将其作为增加诱蚊器捕获量的补充物,对黄颡鱼和密西西比红颡鱼进行了引诱试验。虽然所有引诱剂都能使诱蚊器的捕获率高于对照,但本研究的结果并不能确定一种引诱剂对在佛罗里达州Cedar Key捕获的库蠓更有吸引力。为了开发有效的库蠓成虫诱捕控制系统,需要进一步研究评估不同物种的诱捕器类型和引诱剂。
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引用次数: 0
LABORATORY EVALUATION OF BIGSHOT MAXIM, REPELCARE, AND CLOVE ESSENTIAL OIL, SYZYGIUM AROMATICUM AGAINST THE LONE STAR TICK, AMBLYOMMA AMERICANUM 大针maxim、驱避剂和丁香精油对美洲双头蜱的室内评价
Pub Date : 2023-05-02 DOI: 10.32473/jfmca.70.1.133839
Lea Bangonan, Muhammad Farooq, S. Peper, V. Aryaprema, W. Qualls, R. Xue
Synthetic acaricides have been the most used method for controlling tick populations. However, their frequent application has had negative impacts on treated animals and the environment and led to the development of resistance in tick populations. These factors generated interest and the need to find more environmentally friendly alternatives. In this study, the repellency and acaricidal effects of BigShot Maxim (AIs: cedarwood oil, cinnamon oil, thyme oil), RepelCare (AIs: turmeric oil and eucalyptus oil), and clove oil, Syzygium aromaticum L., were tested against the lone star tick, Amblyomma Americanum (Linnaeus). Despite no evidence of repellency for BigShot Maxim, RepelCare and clove oil, its disruption of the host seeking behavior after contact to the products was observed and further investigated. BigShot Maxim and clove oil were selected for mortality testing and resulted in complete mortality of male, female ticks, and nymphs with some application rates. The results from this study provide a better understanding of repellency and acaricidal effects of botanical products against the lone star tick that can be used to further improve the development of green chemistry. However, further studies are warranted before these botanical products can be implemented as effective alternatives to chemical acaricides to use in integrated vector control.
合成杀螨剂是控制蜱虫数量最常用的方法。然而,它们的频繁使用对治疗动物和环境产生了负面影响,并导致蜱虫种群产生耐药性。这些因素引起了人们的兴趣和寻找更有利于环境的替代办法的需要。研究了BigShot Maxim(雪松油、肉桂油、百里香油)、RepelCare(姜黄油、桉树油)和丁香油(Syzygium aromaticum L.)对美国孤星蜱Amblyomma Americanum (Linnaeus)的驱避和杀螨效果。尽管没有证据表明BigShot Maxim, RepelCare和丁香油具有驱避作用,但观察到其在接触产品后对宿主寻找行为的破坏并进行了进一步研究。以大枪油和丁香油分别进行死亡率试验,在不同施用量下,雄、雌蜱和若虫均完全死亡。本研究结果为进一步了解植物产品对孤星蜱的驱避和杀螨作用提供了理论依据,为进一步促进绿色化学的发展提供了理论依据。然而,在将这些植物产品作为化学杀螨剂的有效替代品用于病媒综合控制之前,有必要进行进一步研究。
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引用次数: 0
EFFECT OF NOZZLE ORIENTATION ON DISPERSION OF TRUCK MOUNTED ULTRA LOW VOLUME SPRAY AT DIFFERENT HEIGHTS AND DISTANCES 喷嘴方向对车载超低体积喷雾在不同高度和距离下分散的影响
Pub Date : 2023-05-02 DOI: 10.32473/jfmca.70.1.133691
M. Farooq, R. Xue, C. Bibbs, J. Cilek, Steven T. Smoleroff
A field evaluation study was conducted to understand the dispersion of spray from a truck-mounted ultra-low-volume (ULV) sprayer when released with the nozzle oriented at the conventional angle of 45° in addition to 22° and 0° with respect to ground surface. The study was conducted in an open grassy field at the Anastasia Mosquito Control District by applying Aqualuer™ 20-20 at the highest label application rate. Mortality of caged female Aedes aegypti was tested at 1.5, 3.0, 6.0, and 8.5 m heights at 0, 30, 60, and 90 m from the spray line. Spray trials were conducted during early morning hours and all test groups were replicated three times over different days. During these evaluations higher mortality was recorded from the 22° and 0° nozzle orientations up to 30 m from the spray line and at heights up to 3 m. The conventional nozzle orientation of 45° provided the greatest mortality 30 m from application source at heights of 6.0 and 8.5 m.
研究人员对车载超低体积(ULV)喷雾器进行了现场评估研究,以了解喷雾器在常规角度45°、22°和0°与地面的夹角下喷射时的喷雾分散情况。研究在阿纳斯塔西娅蚊虫控制区一个开放的草地上进行,以最高的标签使用率使用Aqualuer™20-20。在距喷药线0、30、60和90 m处,分别在1.5、3.0、6.0和8.5 m高度检测埃及伊蚊笼雌蚊的死亡率。喷雾试验在清晨进行,所有试验组在不同的日子重复三次。在这些评估中,从22°和0°喷嘴方向到距离喷雾线30米和高度3米处记录到较高的死亡率。传统的45°喷管方向在距施药源30 m处,在6.0和8.5 m高度处,死亡率最高。
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引用次数: 0
期刊
Journal of the Florida Mosquito Control Association
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