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Total Polyphenol, Flavonoid Contents and Antioxidant Activity of Red Kohlrabi Sprouts 红甘蓝芽总多酚、类黄酮含量及抗氧化活性研究
Pub Date : 2016-06-03 DOI: 10.18143/JISANH_v3i4_1350
Cho Jayong, Ji Okjoo
This study was conducted to clarify the bioactive activities of red kohlrabi sprouts(Brassica oleracea L. var. gongylodes L.), which were produced using the smart farm and drum-type rotary facilities arranged in a row. We have harvested red kohlrabi sprout vegetables at 6 days after germination which were sprouted at 20℃. Roots were harvested at 90 days after seedling, which were fresh weight 752g/ea. Samples were freeze drying to analyse the bioactive components. We have also examined the bioactive activity of sprouts and roots of kohlrabi after extracting with methanol. Total phenol and flavonoid contents of red kohlrabi sprouts increased significantly more than two times than those of roots. Antioxidant enzyme activity of red kohlrabi sprouts such as SOD, CAT, APX and POD also increased remarkably compared with that of roots. DPPH radical scavenging activity and ABTS radical scavenging activity of red kohlrabi sprouts were higher than those of roots. However, there were lower anti-cancer activities of sprouts against Calu-6 and MCF-7 cell lines than those of roots. Key-words : Kohlrabi, sprout, polyphenol, flavonoid, antioxidation, bioactive activity This study was conducted under the supply of "Korea Small and Medium Business Administration(C0353901)".
本研究旨在阐明在智能农场和滚筒式轮转设施中成排生产的红甘蓝芽(Brassica oleracea L. var. gongylodes L.)的生物活性。我们在20℃条件下,收获了发芽后6天的红甘蓝芽菜。幼苗后90天采根,鲜重752g/ea。样品冷冻干燥,分析生物活性成分。我们还研究了甲醇提取后大头菜芽和根的生物活性。红甘蓝芽的总酚和类黄酮含量较根显著提高2倍以上。与根相比,红甘蓝芽的SOD、CAT、APX和POD等抗氧化酶活性也显著提高。红甘蓝芽对DPPH自由基和ABTS自由基的清除能力均高于根。芽对Calu-6和MCF-7细胞系的抗癌活性低于根。关键词:大头菜,芽,多酚,类黄酮,抗氧化,生物活性本研究是在“韩国中小企业厅(C0353901)”资助下进行的。
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引用次数: 0
Manipulation of VEGF-A splicing using natural compounds as a potential therapeutic in diabetic nephropathy 使用天然化合物操纵VEGF-A剪接作为糖尿病肾病的潜在治疗方法
Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1313
M. Stevens, S. Oltean, S. Harper
Alternative splicing of vascular endothelial growth factor (VEGF-A) gives rise to isoform families, the pro-angiogenic VEGF-Axxx and the anti-angiogenic VEGF-Axxxb. VEGF-A165b has previously been shown to be protective in several murine models of renal disease, as well as providing cytoprotection to podocytes in culture. This study aimed to investigate whether a blueberry extract that promotes VEGF-A­165b expression, is a potential therapeutic in a mouse model of type I diabetic nephropathy. The natural extract had no effect on blood glucose levels, but did delay the onset/progression of albuminuria in diabetic mice, and prevented increases in glomerular water permeability. Diabetic control mice had a reduced glomerular expression of nephrin and reduced glomerular capillary circumference, which were rescued when treated with the natural extract. HUVECs displayed reduced tube formation when treated with the natural extract, which was rescued upon addition of Ab-VEGF-A165b. Certain unknown compounds within the blueberry extract are able to switch VEGF-A splicing to promote VEGF-A165b expression in podocytes. The extract plays a protective role in the prevention and treatment of diabetic nephropathy in the DBA/2J type I diabetic mouse model. This study highlights the therapeutic potential of switching VEGF-A splicing to promote VEGF-A165b in diabetic nephropathy.
血管内皮生长因子(VEGF-A)的选择性剪接产生了促血管生成的VEGF-Axxx和抗血管生成的VEGF-Axxxb异构体家族。VEGF-A165b先前已被证明在几种小鼠肾脏疾病模型中具有保护作用,并在培养中为足细胞提供细胞保护。本研究旨在探讨促进VEGF-A-165b表达的蓝莓提取物是否在1型糖尿病肾病小鼠模型中具有潜在的治疗作用。天然提取物对血糖水平没有影响,但确实延缓了糖尿病小鼠蛋白尿的发生/进展,并阻止肾小球水渗透性的增加。糖尿病对照小鼠肾小球nephrin表达降低,肾小球毛细血管周长减小,经天然提取物处理后恢复正常。用天然提取物处理HUVECs时,HUVECs的管状形成减少,添加Ab-VEGF-A165b后,HUVECs的管状形成得以恢复。蓝莓提取物中的某些未知化合物能够改变VEGF-A剪接,从而促进VEGF-A165b在足细胞中的表达。在DBA/2J型糖尿病小鼠模型中,该提取物对糖尿病肾病具有预防和治疗的保护作用。本研究强调了切换VEGF-A剪接促进VEGF-A165b在糖尿病肾病中的治疗潜力。
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引用次数: 0
Fortification of orange juice with antioxidant from orange by-products and evaluation of stability during in vitro digestion 从橙子副产品中提取抗氧化剂强化橙汁及其体外消化稳定性评价
Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1320
Jorge G. Figueroa, Xavier Celi-Bonilla, Jessica Delgado-Sánchez, Soledad González-Arévalo, I. Borrás-Linares, A. Segura‐Carretero
Orange juice production provides large amounts of underutilised by-products that suppose an environmental problem for the food industry, despite their high content in bioactive compounds. The aims of this work have been the fortification of commercial orange juice with bioactive compounds from orange pulp in order to improve its antioxidant activity. Thus, different aqueous orange pulp extracts were encapsulated and incorporated into commercial orange juice at different proportions. The stability of the antioxidant ingredient was followed over 8 weeks. The total phenolic content and the antioxidant capacity of these formulations were measured with different antioxidant procedures. Additionally, the effect of an in-vitro digestion in the antioxidant activity of the fortified juices was studied. The total phenolic content of the developed ingredient decreased in 34 % during the two first weeks of storage, but after that it remain constant. The results revealed that the commercial orange juice fortified with the encapsulated extract from orange pulp showed an increase of total phenolic content of 10-22 %, while the antioxidant activity of the juice improved in all the antioxidant assays. The addition of the antioxidant extract showed a reduction in the decrease of the antioxidant activity of the juices after de in-vitro digestion.
橙汁生产提供了大量未得到充分利用的副产品,尽管它们的生物活性化合物含量很高,但对食品工业造成了环境问题。本研究的目的是在商品橙汁中加入从橙浆中提取的生物活性化合物,以提高其抗氧化活性。因此,不同的水橙浆提取物被封装并以不同的比例掺入商业橙汁。8周以上观察抗氧化成分的稳定性。采用不同的抗氧化工艺,测定了各配方的总酚含量和抗氧化能力。此外,还研究了体外消化对强化果汁抗氧化活性的影响。总酚含量在贮藏前两周内下降34%,贮藏后保持不变。结果表明,经橙浆包封提取物强化后的市售橙汁中总酚含量提高了10 ~ 22%,各项抗氧化指标的抗氧化活性均有所提高。抗氧化提取物的加入对体外消化后果汁抗氧化活性的下降有一定的抑制作用。
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引用次数: 0
Gut microbiome species richness differ after intake of green tea and cranberry extracts by lean and obese mice 瘦小鼠和肥胖小鼠摄取绿茶和蔓越莓提取物后,肠道微生物种类丰富度不同
Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1355
E. Anzenbacherová, J. Nekvindová, L. Jourová, P. Anzenbacher
Green tea and cranberry are rich with phenolic compounds and they are extensively studied for their cardioprotective, anti-carcinogenic and anti-infective activities. This study analyzes their effect on gut microbiota and selected biochemical parameters in lean NMRI mice and in monosodium glutamate (MSG) – induced mouse model of obesity with insulin resistance and hyperinsulinemia. Results on the species richness show similarity of the groups that were fed by standard chow and the diet enriched with green tea extract, both in the normal (lean) mice and those with MSG-induced obesity. On the contrary, mice treated with cranberry extract tend to form a separate cluster of data points, showing a more pronounced difference between this group and control and green tea-treated groups. Again, the shift is present in both normal and obese group of mice. In other words, the analysis of species richness indicate a lowering of the number of taxonomic units after intake of cranberry extract; this effect was even more apparent in obese mice. On the contrary, the green tea extract did not cause a prominent change of the species richness of the mice gut microbiome. Supported by GACR303/12/G163.
绿茶和蔓越莓富含酚类化合物,它们具有保护心脏、抗癌和抗感染的活性,被广泛研究。本研究分析了它们对精瘦NMRI小鼠和味精(MSG)诱导的肥胖伴胰岛素抵抗和高胰岛素血症小鼠模型肠道微生物群和部分生化参数的影响。物种丰富度的结果显示,在正常(瘦)小鼠和味精诱导的肥胖小鼠中,喂食标准食物和富含绿茶提取物的饮食组的物种丰富度相似。相反,用蔓越莓提取物治疗的小鼠倾向于形成一个单独的数据点簇,显示出这一组与对照组和绿茶治疗组之间更明显的差异。同样,这种变化在正常组和肥胖组的老鼠中都存在。换言之,物种丰富度分析表明,摄取蔓越莓提取物后,分类单位数量降低;这种效果在肥胖老鼠身上更为明显。相反,绿茶提取物没有引起小鼠肠道微生物群落物种丰富度的显著变化。GACR303/12/G163支持。
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引用次数: 0
Polyphenols bioavailability in piglets fed with grape pomace diet 葡萄渣饲粮中多酚类物质的生物利用度
Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1357
V. Chedea, R. Pelmuș, M. Rotar, M. Gras, M. Hăbeanu, I. Țăranu
The aim of the present study was to evaluate the influence of a diet containing 5% grape pomace (GP) on polyphenols absorption and their antioxidant antiradicalic activity (DPPH test) on weaning piglets. 20 crossbred TOPIG starter piglets were assigned to one of the 2 treatments: control (10 piglets- normal diet for weaned pig-C) and diet with GP (10 piglets -diet -D) for 36 days. After 15 days and at the end of the experimental period, blood samples were collected from the two groups. All piglets were slaughtered and the organs, liver, spleen, duodenum, colon, kidney, Longissimus dorsi muscle, mesenteric lymph nodes, heart and brain were collected. The total polyphenols (TP) content and the antioxidant activity of the feed diet and diet components as well as of the blood and organs were determined by using Folin Ciocalteu and DPPH test respectively. GP increased the TP content in feed as well as its antioxidant activity. Expressed as % of control, TP were found to be higher in plasma, duodenum, colon, kidneys and heart than in the control. The highest antioxidant activity was found in liver, kidneys and heart.
本试验旨在研究饲粮中添加5%葡萄渣对断奶仔猪多酚吸收及其抗氧化抗自由基活性(DPPH)的影响。将20头杂交TOPIG起始仔猪分为对照组(10头仔猪-正常断奶仔猪饲粮c)和添加GP饲粮(10头仔猪饲粮d),试验期36 d。15 d后和试验期结束时,采集两组小鼠的血液样本。所有仔猪均屠宰,取脏器、肝、脾、十二指肠、结肠、肾、背最长肌、肠系膜淋巴结、心、脑。采用Folin Ciocalteu法和DPPH法分别测定饲料、饲料组分、血液和脏器中总多酚(TP)含量和抗氧化活性。GP提高了饲料中TP的含量,提高了饲料的抗氧化活性。血浆、十二指肠、结肠、肾脏和心脏的TP含量均高于对照组。肝脏、肾脏和心脏的抗氧化活性最高。
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引用次数: 0
Genetic polymorphisms of CYP enzymes and its influence on drug interactions between isoflavonoid genistein and model drugs CYP酶的遗传多态性及其对异黄酮染料木素与模型药物相互作用的影响
Pub Date : 2016-06-03 DOI: 10.18143/JISANH_v3i4_1351
P. Anzenbacher, M. Zapletalová, Kristýna Krasulová, E. Anzenbacherová
Genetic polymorphism in cytochrome P450 (CYP) family of enzymes is a rather frequent cause of serious problems with proper dosing of drugs. This effect may be augmented by drug interaction between concomitantly taken drugs which are metabolized by the same enzymes. The second interacting partner however may easily be a compound of a natural origin as it is in the case of flavonoids from the grapefruit juice. We examined the effect of a prototypic isoflavonoid, genistein, on metabolism of probe drug substrates in genotypized human liver microsomes corresponding to slow, extensive or intermediate metabolizers with respect to CYP. The results document the most prominent effect of genistein and CYP2C19*2 allele on activity of this enzyme in slow metabolizers (down to aprox. 10%); the least effect was observed with CYP2D6*4 and CYP2D6*41 alleles (to about 60% of control value). In other words, inhibition of CYP activities by isoflavonoid (genistein) may be easily augmented by presence of defective alleles in patients of phenotypes of slow or intermediate metabolizers. Supported by a GACR P303/12/G163 Center of Excellence Project and by a Palacky University student´s project IGA_LF_2016_006.
细胞色素P450 (CYP)家族酶的遗传多态性是导致药物剂量严重问题的常见原因。这种作用可能会因同时服用的药物之间的相互作用而增强,这些药物由相同的酶代谢。然而,第二个相互作用的伙伴可能很容易是一种天然来源的化合物,就像葡萄柚汁中的类黄酮一样。我们研究了一种原型异黄酮染料木素对基因型人肝微粒体中探针药物底物代谢的影响,这些基因型人肝微粒体对应于CYP的缓慢、广泛或中间代谢。结果表明,染料木素和CYP2C19*2等位基因对慢代谢者(低至约约)该酶活性的影响最为显著。10%);CYP2D6*4和CYP2D6*41等位基因影响最小(约为对照值的60%)。换句话说,异黄酮(染料木素)对CYP活性的抑制可能很容易因存在缺陷等位基因而在缓慢或中等代谢表型的患者中增强。GACR P303/12/G163卓越中心项目和Palacky大学学生项目IGA_LF_2016_006资助。
{"title":"Genetic polymorphisms of CYP enzymes and its influence on drug interactions between isoflavonoid genistein and model drugs","authors":"P. Anzenbacher, M. Zapletalová, Kristýna Krasulová, E. Anzenbacherová","doi":"10.18143/JISANH_v3i4_1351","DOIUrl":"https://doi.org/10.18143/JISANH_v3i4_1351","url":null,"abstract":"Genetic polymorphism in cytochrome P450 (CYP) family of enzymes is a rather frequent cause of serious problems with proper dosing of drugs. This effect may be augmented by drug interaction between concomitantly taken drugs which are metabolized by the same enzymes. The second interacting partner however may easily be a compound of a natural origin as it is in the case of flavonoids from the grapefruit juice. We examined the effect of a prototypic isoflavonoid, genistein, on metabolism of probe drug substrates in genotypized human liver microsomes corresponding to slow, extensive or intermediate metabolizers with respect to CYP. The results document the most prominent effect of genistein and CYP2C19*2 allele on activity of this enzyme in slow metabolizers (down to aprox. 10%); the least effect was observed with CYP2D6*4 and CYP2D6*41 alleles (to about 60% of control value). In other words, inhibition of CYP activities by isoflavonoid (genistein) may be easily augmented by presence of defective alleles in patients of phenotypes of slow or intermediate metabolizers. Supported by a GACR P303/12/G163 Center of Excellence Project and by a Palacky University student´s project IGA_LF_2016_006.","PeriodicalId":17323,"journal":{"name":"Journal of the International Society of Antioxidants in Nutrition & Health","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81602031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Semi-preparative HPLC isolation of bioactive compounds from Lippia citriodora extract: a useful nutraceutical approach 半制备高效液相色谱法分离柠檬黄提取物中的生物活性化合物:一种实用的营养保健品方法
Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1359
M. L. Cádiz-Gurrea, María Elena Alañón-Pardo, D. Arráez-Román, S. Fernández-Arroyo, A. Segura‐Carretero
Lippia citriodora (lemon verbena), a shrub indigenous to South America, was introduced into Europe at the end of the 17th century and has been widely used in infusions for its antispasmodic, antipyretic, sedative, and digestive properties. Furthermore, this plant is used in the food industry to flavour different products. Lemon verbena infusion contains significant amounts of polyphenols with healthy properties including high antioxidant activity1. Nevertheless, a better knowledge of the phenolic composition from lemon verbena polyphenols could be useful for nutraceutical development. In this work, the compounds in the L. citriodora extract were isolated by semipreparative HPLC according to their elution order. A total of 11 fractions were collected and the solvent was evaporated under vacuum. The residue of each fraction was weighted and dissolved with an appropriate volume of water at concentration level of 100 g/mL. Finally, fractions were filtered through a 0.25 μm filter. Afterwards, the composition of each fraction was established by a detailed HPLC-ESI/DAD-TOF-MS analysis2. A total of 30 compounds were identified in the L. citriodora extract belonging to various structural classes such as iridoids, flavonoids and phenylethanoids, most of them with a demonstrated antoxidant, anti-inflammatory and anticancer activities.
柠檬马鞭草(Lippia citriodora)是一种原产于南美洲的灌木,于17世纪末被引入欧洲,因其抗痉挛、解热、镇静和消化的特性而被广泛用于输液。此外,这种植物在食品工业中用于调味不同的产品。柠檬马鞭草注射液含有大量的多酚,具有健康的特性,包括高抗氧化活性1。然而,更好地了解柠檬马鞭草多酚的酚类成分可能有助于营养保健的开发。本研究采用半制备高效液相色谱法,对香茅提取物中的化合物按洗脱顺序进行分离。共收集11个馏分,真空蒸发溶剂。将各馏分的残渣称重,用适当体积的水以100 g/mL的浓度溶解。最后,通过0.25 μm过滤器过滤馏分。然后,通过详细的HPLC-ESI/DAD-TOF-MS分析确定各组分的组成2。从枸橼提取物中鉴定出环烯醚萜类、黄酮类和苯乙醇类等30种化合物,其中大部分具有抗氧化、抗炎和抗癌活性。
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引用次数: 0
Effect of flavonoids on cytochrome P450 activity in the gut – an in vitro food-drug interaction study 黄酮类化合物对肠道细胞色素P450活性的影响——体外食药相互作用研究
Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1325
O. Farkas, D. Kovács, Z. Karancsi, G. Csikó, O. Palócz
Cytochrome P450 enzymes have a remarkable role in the xenobiotics metabolism. Oxidation of drugs and food components catalysed by the same isoenzyme can lead to drug-food interactions. Greatest quantity of CYP enzymes can be found in the liver. Nevertheless, the intestinal CYP garniture also contributes to the metabolism of clinically important drugs. Our research focused on the effects of flavonoids on the intestinal CYP enzymes. Intestinal epithelial cells (IPEC-J2) were treated by apigenin and apigenin-trimethylether. Phenobarbital was used as CYP inducer, mixture of naphtoflavone and ketoconazole as inhibitor. The CYP1A1, CYP1A2 and CYP3A activities were measured by chemiluminescent assay. Treatment of enterocytes with antipyrine was also performed in order to test possible drug interactions. Both flavones worked as significant CYP3A4 inhibitors. There was no significant difference between the inhibitory effects of the flavones at the same dose. However, apigenin-trimethylether combined with the inducer, was a more potent inhibitor, than the apigenin. Antipyrine decreased the enzymatic activity, which effect was enhanced, when antipyrine and apigenin were administered simultaneously. In conclusion, our results suggest that both apigenin and its trimethylated derivative can inhibit the intestinal CYP3A, which is the most important role in the metabolism of clinically relevant drugs.
细胞色素P450酶在体外代谢中具有重要作用。由同一同工酶催化的药物和食品成分氧化可导致药物-食品相互作用。CYP酶在肝脏中含量最高。然而,肠道CYP结构也有助于临床重要药物的代谢。我们的研究重点是黄酮类化合物对肠道CYP酶的影响。芹菜素和芹菜素-三甲基醚分别处理小肠上皮细胞IPEC-J2。以苯巴比妥为CYP诱导剂,萘黄酮与酮康唑的混合物为抑制剂。化学发光法测定CYP1A1、CYP1A2和CYP3A活性。为了测试可能的药物相互作用,还进行了安替比林肠细胞治疗。这两种黄酮都是CYP3A4抑制剂。在相同剂量下,黄酮类化合物的抑制作用无显著差异。然而,芹菜素-三甲基醚与诱导剂结合,是一个更有效的抑制剂,比芹菜素。安替比林降低了酶活性,当安替比林和芹菜素同时给药时,这种作用增强。综上所述,我们的研究结果表明,芹菜素及其三甲基化衍生物都可以抑制肠道CYP3A,这是临床相关药物代谢中最重要的作用。
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引用次数: 1
Evaluation of the effect of resveratrol isolated and in combination with tamoxifen on viability of breast cancer cells. 白藜芦醇分离及联合他莫昔芬对乳腺癌细胞活力的影响。
Pub Date : 2016-06-03 DOI: 10.18143/JISANH_V3I4_1356
Beatriz Tinoco Franceschi, M. R. Torqueti
Breast cancer is treated with tamoxifen, an antagonist classified as selective estrogen receptor modulator, widely used for the first-line treatment of breast cancer. Resveratrol, a polyphenol present in the diet, has received attention due to its beneficial biological properties. There is great demand of science for new therapeutic targets for the treatment of breast cancer. Thus, evaluate cellular cytotoxicity of resveratrol, desmethyltamoxifen and their combination by the method MTT assay in culture estrogen-dependent breast cancer cells (MCF -7) and estrogen-independent (MDA-MB -231) will allow assess whether resveratrol provides benefits for the treatment of breast cancer. Our results indicate that the combination of resveratrol with tamoxifen reduced the viability of additive manner in breast cancer cells.
它莫昔芬是一种被归类为选择性雌激素受体调节剂的拮抗剂,广泛用于乳腺癌的一线治疗。白藜芦醇是一种存在于饮食中的多酚,由于其有益的生物学特性而受到关注。科学对乳腺癌治疗的新靶点有很大的需求。因此,在培养的雌激素依赖型乳腺癌细胞(MCF -7)和雌激素非依赖型乳腺癌细胞(MDA-MB -231)中,通过MTT法评估白藜芦醇、去甲基他莫昔芬及其组合的细胞毒性,将有助于评估白藜芦醇是否对乳腺癌的治疗有益。我们的研究结果表明,白藜芦醇与他莫昔芬联合使用可降低乳腺癌细胞的活性。
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引用次数: 0
MODULATION OF IMMUNE AND ANTIOXIDANT RESPONSE IN WEANED PIGLETS BY BIOACTIVE COMPOUNDS FROM GRAPE SEED BY-PRODUCT 葡萄籽副产品生物活性化合物对断奶仔猪免疫和抗氧化反应的调节作用
Pub Date : 2016-06-03 DOI: 10.18143/JISANH_v3i4_1354
I. Țăranu, I. Grosu, A. Gras, M. Palade, V. Chedea, D. Marin
Grape processing for the production of wine generates a wide range of residues (pomace and seeds) rich in bioactive compounds (polyphenols, etc). Their biological potential has been already valorised in ruminant nutrition, but has been little investigated in pigs. This study was conducted to analyse the effect of dietary inclusion of grape seed by-product on growth performance, immune and antioxidant function in weaned pigs. A total of 12 weaned pigs were fed with a control or 5% grape seed cakes (GS) diets for 30 days. Pigs were sacrificed after 30 days, blood and organs were collected and stored at –80°C until analyses. The results showed that dietary GS did not exert significant effect on pig performance, but generated an anti-inflammatory effect by decreasing gene expression of TNF-α, IL-6, IFN-γ and other inflammatory molecules in liver. No effect on the activity and gene expression of antioxidant enzymes and malondialdehyde was also observed in liver. The effect on inflammation was less evident in spleen, but a slight increase in antioxidant enzyme activity was noticed. In conclusion, the dietary inclusion of GS modulated differentially immune and antioxidant response in weaned pig.
葡萄加工生产葡萄酒会产生各种各样的残留物(果渣和种子),这些残留物富含生物活性化合物(多酚等)。它们的生物学潜力已经在反刍动物营养中得到了证实,但在猪身上的研究却很少。本试验旨在分析饲粮中添加葡萄籽副产物对断奶仔猪生长性能、免疫和抗氧化功能的影响。试验选取12头断奶仔猪,分别饲喂对照组和5%葡萄籽饼(GS)饲粮,饲喂30 d。30天后处死猪,采集血液和器官,-80°C保存,待分析。结果表明,饲粮中添加GS对猪生产性能无显著影响,但通过降低肝脏中TNF-α、IL-6、IFN-γ等炎性分子的基因表达而产生抗炎作用。对肝脏中抗氧化酶和丙二醛的活性和基因表达没有影响。对脾脏炎症的影响不明显,但抗氧化酶活性略有增加。综上所述,饲粮中添加GS可调节断奶仔猪的差异免疫和抗氧化反应。
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引用次数: 0
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Journal of the International Society of Antioxidants in Nutrition & Health
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