Geobiology最新文献

Upper Messinian carbonates recently recorded in the Salento Peninsula (southern Italy, central Mediterranean) contain microbial facies, including textures never previously described in the Late Miocene of the Mediterranean. This study focuses on the geometry and internal fabrics of a 3 × 28 m build-up of coalescent dendrolite and thrombolite, to examine its formation and the possible microbes involved, and to reconstruct its growth dynamics and related palaeoenvironmental conditions. Salento dendrolites have centimetric dendritic growth forms with a microlaminated, originally aragonitic, microstructure. The thrombolites, in contrast, are characterized by larger mesoclots with arborescent, anastomose growth patterns and a distinctive microfabric of small, originally calcitic, spheroids with a sparry nucleus surrounded by acicular crystals. Bio-geochemical analyses (UV epifluorescence, micro-Raman spectroscopy and SEM-EDS) reveal the presence of organic matter intimately associated with both dendrolite and thrombolite textures, supporting a biotic origin. The sedimentary context and microfabrics suggest that cyanobacteria may have played a major role in the formation of these structures, together with heterotrophic microbes, mainly sulfate-reducing bacteria, in the dendrolite. Build-up geometries, stratigraphic setting, and analysis of the associated sediment suggest that the dendrolite-thrombolite framework developed in a small, shallow-water lagoon, under moderate to high energy, variable salinity, and possibly high sedimentation rate. Salento dendrolite-thrombolite build-up appears to be the only known example of large microbial bioconstruction made by microlaminated dendrolites.

Microscopic tubules and filaments composed of iron minerals occur in various rock types of all ages. Although typically lacking carbonaceous matter, many are reasonably interpreted as the remains of filamentous microorganisms coated with crystalline iron oxyhydroxides. Iron-oxidizing bacteria (IOB) acquire such a coating naturally during life. However, recent debates about purported microfossils have highlighted the potential for self-organized nonbiological mineral growth (particularly in chemical gardens) to form compositionally and morphologically similar tubules. How can biogenic and abiogenic iron-mineral tubules be differentiated? Here, we use optical and electron microscopy and Mössbauer spectroscopy to compare the composition, microtexture, and morphology of ferruginous chemical gardens and iron-mineralized sheaths of bacteria in the genus Leptothrix. Despite broad morphological similarity, we find that Leptothrix exhibits a narrower range of filament diameters and lower filament tortuosity than chemical gardens. Chemical gardens produced from a ferrous salt also tend to incorporate Fe²⁺ whereas Leptothrix sheaths predominantly do not. Finally, the oxyhydroxides formed in Leptothrix sheaths tend to be smoother and denser on the inward-facing side, rougher and sparser on the outward side, whereas for chemical garden tubules the reverse is true. Some of these differences show promise for the diagnosis of natural samples.

Silicification of microfossils is an important taphonomic process that provides a record of microbial life across a range of environments throughout Earth history. However, questions remain regarding the mechanism(s) by which silica precipitated and preserved delicate organic material and detailed cellular morphologies. Constraining the different mechanisms of silica precipitation and identifying the common factors that allow for microfossil preservation is the key to understanding ancient microbial communities and fossil-preserving mechanisms. Here, we use synchrotron ptychographic X-ray computed tomography (PXCT) as a novel technique to analyze microfossils from the Cretaceous Barra Velha Formation and better characterize their diverse morphologies and preservation styles. Through this technique, we generate 2D and 3D reconstructions that illustrate the microfossils and silica-organic textures at nanometer resolution. At this resolution, we identify previously uncharacterized silica textures and organic-silica relationships that help us relate findings from modern silicifying environments and experimental work to the fossil record. Additionally, we identify primary morphological differences among the microfossils as well as preservational variability that may have been driven by physiological and/or biochemical differences between the different organisms that inhabited the Cretaceous pre-salt basin. These findings help us to better characterize the diversity and complexity of the microbiota in this ancient basin as well as taphonomic processes and biases that may have driven microfossil preservation in this and other silicifying environments throughout Earth history.

Shallow-marine environments are thought to have been pivotal to the spreading, perhaps even the origin, of early life on Earth. The shallow-marine Archean sedimentary record of early life, however, is biased towards carbonates; nearshore siliciclastic environments have not received proportional attention. Here we describe densely laminated, silicified and dolomitized fossil calcareous mounds in tidal-facies sandstones of the Archean Moodies Group (ca. 3.22 Ga) in the Barberton Greenstone Belt, Eswatini. They vary between (1) cm- to dm-scale, isolated, club- to pedestal-shaped, nodular mounds on top of and within the conduits of fluid-escape structures, and (2) mm- to cm-scale, undulatory and wavily laminated structures, interbedded with well-bedded silt- and sandstones. Geochemical indicators of a possible biogenic origin were largely obliterated by local hydrothermal alteration and regional lower-greenschist-facies metamorphism: In situ SIMS δ¹³C_carb isotope analyses from several traverses across the best-preserved laminae of a mound and δ³⁴S_VCDT values from diagenetic rims of nearby detrital pyrite grains yield ambiguous isotopic evidence about biologic processing; TOC of putative laminae is too low to measure δ¹³C_org, and Raman spectroscopy of finely dispersed carbonaceous particles and of kerogenous laminae indicate mean maximum metamorphic temperature of ca. 500°C. Textural and regional evidence, however, suggests that the carbonate laminae represent metabolic products of microbial communities that took advantage of sand volcanoes from which nutrient-rich fluids erupted episodically. We base this inference on the habitable depositional setting on a wave- or current-swept photic-zone tidal platform, the stromatolitic morphologies in two and three dimensions, the occurrence of in-situ kerogen, the carbonate mineralogy, and the presence of comparable mound structures elsewhere in the Moodies Group. Although the metabolic strategies utilized by the microorganisms remain unknown, this occurrence places a novel ecologic niche in the Paleoarchean microbial colonization of coastal regions.

Alkaline lakes are among the most bioproductive aquatic ecosystems on Earth. The factors that ultimately limit productivity in these systems can vary, but nitrogen (N) cycling in particular has been shown to be adversely affected by high salinity, evidently due to the inhibition of nitrifying bacteria (i.e., those that convert ammonic species to nitrogen oxides). The coastal plain of Coorong National Park in South Australia, which hosts several alkaline lakes along 130 km of coastline, provides an ideal natural laboratory for examining how fine-scale differences in the geochemistry of such environments can lead to broad variations in nitrogen cycling through time, as manifest in sedimentary δ¹⁵N. Moreover, the lakes provide a gradient of aqueous conditions that allows us to assess the effects of pH, salinity, and carbonate chemistry on the sedimentary record. We report a wide range of δ¹⁵N values (3.8‰–18.6‰) measured in the sediments (0–35 cm depth) of five lakes of the Coorong region. Additional data include major element abundances, carbonate δ¹³C and δ¹⁸O values, and the results of principal component analyses. Stable nitrogen isotopes and wt% sodium (Na) display positive correlation (R² = 0.59, p < 0.001) across all lake systems. Principal component analyses further support the notion that salinity has historically impacted nitrogen cycling. We propose that the inhibition of nitrification at elevated salinity may lead to the accumulation of ammonic species, which, when exposed to the water column, are prone to ammonia volatilization facilitated by intervals of elevated pH. This process is accompanied by a significant isotope fractionation effect, isotopically enriching the nitrogen that remains in the lake water. This nitrogen is eventually buried in the sediments, preserving a record of these combined processes. Analogous enrichments in the rock record may provide important constraints on past chemical conditions and their associated microbial ecologies. Specifically, ancient terrestrial aquatic systems with high δ¹⁵N values attributed to denitrification and thus oxygen deficiency may warrant re-evaluation within the framework of this alternative. Constraints on pH as provided by elevated δ¹⁵N via ammonia volatilization may also inform critical aspects of closed-basin paleoenvironments and their suitability for a de novo origin of life.

Previous studies have documented the presence of phosphite, a reduced and highly soluble form of phosphorus, in serpentinites, which has led to the hypothesis that serpentinizing hydrothermal vents could have been an important source of bioavailable phosphorus for early microbial communities in the Archean. Here, we test this hypothesis by evaluating the genomic hallmarks of phosphorus usage in microbial communities living in modern hydrothermal vents with and without influence from serpentinization. These genomic analyses are combined with results from a geochemical model that calculates phosphorus speciation during serpentinization as a function of temperature, water:rock ratio, and lithology at thermodynamic equilibrium. We find little to no genomic evidence of phosphite use in serpentinizing environments at the Voltri Massif or the Von Damm hydrothermal field at the Mid Cayman Rise, but relatively more in the Lost City hydrothermal field, Coast Range Ophiolite Microbial Observatory, The Cedars, and chimney samples from Old City hydrothermal field and Prony Bay hydrothermal field, as well as in the non-serpentinizing hydrothermal vents at Axial Seamount. Geochemical modeling shows that phosphite production is favored at ca 275°C–325°C and low water:rock ratios, which may explain previous observations of phosphite in serpentinite rocks; however, most of the initial phosphate is trapped in apatite during serpentinization, suppressing the absolute phosphite yield. As a result, phosphite from serpentinizing vents could have supported microbial growth around olivine minerals in chimney walls and suspended aggregates, but it is unlikely to have fueled substantial primary productivity in diffusely venting fluids during life's origin and evolution in the Archean unless substrates equivalent to dunites (composed of > 90 wt% olivine) were more common.

Methanogenic archaea were likely among the earliest organisms to populate the Earth, perhaps contributing to the Archaean greenhouse effect; they are also widely discussed as analogues to any potential life on Mars. However, fossil evidence of archaea has been difficult to identify in the rock record, perhaps because their preservation potential is intrinsically low or because they are particularly small and difficult to identify. Here, we examined the preservation potential of a methanogen of the genus Methanobacterium, recently isolated from a low-temperature serpentinizing system, an environment somewhat analogous to habitats on the early Earth and Mars. Notably, this organism has a cell wall composed of peptidoglycan-like pseudomurein, which may imply a mineralisation potential similar to that of gram-positive bacteria. Methanobacterium cells were placed in carbonate, phosphate, and silicate solutions for up to 3 months in order to assess the relative tendency of these minerals to encrust and preserve cellular morphology. Cells readily acquired a thick, uniform coating of silica, enhancing their potential for long-term preservation while also increasing overall filament size, an effect that may aid the discovery of fossil archaea while hindering their interpretation. Phosphates precipitated from the medium in all experimental setups and even in parallel experiments set up with low-phosphate medium, suggesting a hitherto unknown biomineralisation capacity of methanogens. Carbonate precipitates did not form in close association with cells.

The microbially mediated replacement of sulfate-bearing evaporites by authigenic carbonate and native sulfur under anoxic conditions is poorly understood. Sulfur-bearing carbonates from the Monte Palco ridge (Sicily) replacing Messinian gypsum were therefore studied to better characterize the involved microorganisms. The lack of (1) sedimentary bedding, (2) lamination, and (3) significant water-column-derived lipid biomarkers in the secondary carbonates implies replacement after gypsum deposition (epigenesis). Allochthonous clasts from the older Calcare di Base and the younger Trubi Formation within these carbonates further evidence epigenetic formation. The sulfur-bearing carbonates are significantly ¹³C-depleted (δ¹³C as low as −51‰), identifying methane as a major carbon source. The ¹⁸O-enrichment of the carbonates (δ¹⁸O as high as 5.4‰) probably reflects precipitation from ¹⁸O-enriched fluids transported along adjacent faults or precipitation in a closed system with very little water. Native sulfur with variable ³⁴S-enrichment (δ³⁴S as high as 18.9‰), a relatively small maximum offset (12.3‰) between the sulfate source (gypsum) and native sulfur, and high δ³⁴S values of carbonate-associated sulfate (as high as 61.1‰) suggest a high conversion to native sulfur in a (semi-)closed system, with insignificant sulfate removal. Anaerobic methanotrophic archaea (ANME) apparently affiliated with the ANME-1 clade mediated secondary mineral formation as evidenced by the biomarker inventory, which contains abundant ¹³C-depleted isoprenoids including sn3-hydroxyarchaeol as the sole hydroxyarchaeol isomer and glycerol dibiphytanyl glycerol tetraethers (GDGTs). A series of various, tentatively identified ¹³C-depleted non-isoprenoidal dialkyl glycerol diethers (DAGEs), 10me-C₁₆ fatty acid, hydroxy C₁₆ fatty acids, and cyclopropyl-C_17:0ω7,8 fatty acid agree with sulfate-reducing bacteria participating in the anaerobic oxidation of methane. Specific conditions during gypsum replacement, unlike those at marine methane seeps, are reflected by the occurrence of ¹³C-depleted lipids such as lycopane, 9me-C₁₇ fatty acid, and novel DAGEs. As a response to a confined environment probably characterized by high sulfate concentrations, sulfidic conditions, and elevated salinity, ANMEs and sulfate-reducing bacteria apparently adapted their membrane compositions to cope with such stressors.