Geobiology最新文献

Alkaline lakes are among the most bioproductive aquatic ecosystems on Earth. The factors that ultimately limit productivity in these systems can vary, but nitrogen (N) cycling in particular has been shown to be adversely affected by high salinity, evidently due to the inhibition of nitrifying bacteria (i.e., those that convert ammonic species to nitrogen oxides). The coastal plain of Coorong National Park in South Australia, which hosts several alkaline lakes along 130 km of coastline, provides an ideal natural laboratory for examining how fine-scale differences in the geochemistry of such environments can lead to broad variations in nitrogen cycling through time, as manifest in sedimentary δ¹⁵N. Moreover, the lakes provide a gradient of aqueous conditions that allows us to assess the effects of pH, salinity, and carbonate chemistry on the sedimentary record. We report a wide range of δ¹⁵N values (3.8‰–18.6‰) measured in the sediments (0–35 cm depth) of five lakes of the Coorong region. Additional data include major element abundances, carbonate δ¹³C and δ¹⁸O values, and the results of principal component analyses. Stable nitrogen isotopes and wt% sodium (Na) display positive correlation (R² = 0.59, p < 0.001) across all lake systems. Principal component analyses further support the notion that salinity has historically impacted nitrogen cycling. We propose that the inhibition of nitrification at elevated salinity may lead to the accumulation of ammonic species, which, when exposed to the water column, are prone to ammonia volatilization facilitated by intervals of elevated pH. This process is accompanied by a significant isotope fractionation effect, isotopically enriching the nitrogen that remains in the lake water. This nitrogen is eventually buried in the sediments, preserving a record of these combined processes. Analogous enrichments in the rock record may provide important constraints on past chemical conditions and their associated microbial ecologies. Specifically, ancient terrestrial aquatic systems with high δ¹⁵N values attributed to denitrification and thus oxygen deficiency may warrant re-evaluation within the framework of this alternative. Constraints on pH as provided by elevated δ¹⁵N via ammonia volatilization may also inform critical aspects of closed-basin paleoenvironments and their suitability for a de novo origin of life.

Previous studies have documented the presence of phosphite, a reduced and highly soluble form of phosphorus, in serpentinites, which has led to the hypothesis that serpentinizing hydrothermal vents could have been an important source of bioavailable phosphorus for early microbial communities in the Archean. Here, we test this hypothesis by evaluating the genomic hallmarks of phosphorus usage in microbial communities living in modern hydrothermal vents with and without influence from serpentinization. These genomic analyses are combined with results from a geochemical model that calculates phosphorus speciation during serpentinization as a function of temperature, water:rock ratio, and lithology at thermodynamic equilibrium. We find little to no genomic evidence of phosphite use in serpentinizing environments at the Voltri Massif or the Von Damm hydrothermal field at the Mid Cayman Rise, but relatively more in the Lost City hydrothermal field, Coast Range Ophiolite Microbial Observatory, The Cedars, and chimney samples from Old City hydrothermal field and Prony Bay hydrothermal field, as well as in the non-serpentinizing hydrothermal vents at Axial Seamount. Geochemical modeling shows that phosphite production is favored at ca 275°C–325°C and low water:rock ratios, which may explain previous observations of phosphite in serpentinite rocks; however, most of the initial phosphate is trapped in apatite during serpentinization, suppressing the absolute phosphite yield. As a result, phosphite from serpentinizing vents could have supported microbial growth around olivine minerals in chimney walls and suspended aggregates, but it is unlikely to have fueled substantial primary productivity in diffusely venting fluids during life's origin and evolution in the Archean unless substrates equivalent to dunites (composed of > 90 wt% olivine) were more common.

Methanogenic archaea were likely among the earliest organisms to populate the Earth, perhaps contributing to the Archaean greenhouse effect; they are also widely discussed as analogues to any potential life on Mars. However, fossil evidence of archaea has been difficult to identify in the rock record, perhaps because their preservation potential is intrinsically low or because they are particularly small and difficult to identify. Here, we examined the preservation potential of a methanogen of the genus Methanobacterium, recently isolated from a low-temperature serpentinizing system, an environment somewhat analogous to habitats on the early Earth and Mars. Notably, this organism has a cell wall composed of peptidoglycan-like pseudomurein, which may imply a mineralisation potential similar to that of gram-positive bacteria. Methanobacterium cells were placed in carbonate, phosphate, and silicate solutions for up to 3 months in order to assess the relative tendency of these minerals to encrust and preserve cellular morphology. Cells readily acquired a thick, uniform coating of silica, enhancing their potential for long-term preservation while also increasing overall filament size, an effect that may aid the discovery of fossil archaea while hindering their interpretation. Phosphates precipitated from the medium in all experimental setups and even in parallel experiments set up with low-phosphate medium, suggesting a hitherto unknown biomineralisation capacity of methanogens. Carbonate precipitates did not form in close association with cells.

The microbially mediated replacement of sulfate-bearing evaporites by authigenic carbonate and native sulfur under anoxic conditions is poorly understood. Sulfur-bearing carbonates from the Monte Palco ridge (Sicily) replacing Messinian gypsum were therefore studied to better characterize the involved microorganisms. The lack of (1) sedimentary bedding, (2) lamination, and (3) significant water-column-derived lipid biomarkers in the secondary carbonates implies replacement after gypsum deposition (epigenesis). Allochthonous clasts from the older Calcare di Base and the younger Trubi Formation within these carbonates further evidence epigenetic formation. The sulfur-bearing carbonates are significantly ¹³C-depleted (δ¹³C as low as −51‰), identifying methane as a major carbon source. The ¹⁸O-enrichment of the carbonates (δ¹⁸O as high as 5.4‰) probably reflects precipitation from ¹⁸O-enriched fluids transported along adjacent faults or precipitation in a closed system with very little water. Native sulfur with variable ³⁴S-enrichment (δ³⁴S as high as 18.9‰), a relatively small maximum offset (12.3‰) between the sulfate source (gypsum) and native sulfur, and high δ³⁴S values of carbonate-associated sulfate (as high as 61.1‰) suggest a high conversion to native sulfur in a (semi-)closed system, with insignificant sulfate removal. Anaerobic methanotrophic archaea (ANME) apparently affiliated with the ANME-1 clade mediated secondary mineral formation as evidenced by the biomarker inventory, which contains abundant ¹³C-depleted isoprenoids including sn3-hydroxyarchaeol as the sole hydroxyarchaeol isomer and glycerol dibiphytanyl glycerol tetraethers (GDGTs). A series of various, tentatively identified ¹³C-depleted non-isoprenoidal dialkyl glycerol diethers (DAGEs), 10me-C₁₆ fatty acid, hydroxy C₁₆ fatty acids, and cyclopropyl-C_17:0ω7,8 fatty acid agree with sulfate-reducing bacteria participating in the anaerobic oxidation of methane. Specific conditions during gypsum replacement, unlike those at marine methane seeps, are reflected by the occurrence of ¹³C-depleted lipids such as lycopane, 9me-C₁₇ fatty acid, and novel DAGEs. As a response to a confined environment probably characterized by high sulfate concentrations, sulfidic conditions, and elevated salinity, ANMEs and sulfate-reducing bacteria apparently adapted their membrane compositions to cope with such stressors.

Microbialites are organo-sedimentary structures formed throughout most of the Earth history, over a wide range of geological contexts, and under a multitude of environmental conditions affecting their composition. The carbon and oxygen isotope records of carbonates, which are most often their main constituents, have been used as a widespread tool for paleoenvironmental reconstructions. However, the multiplicity of factors that influence microbialites formation is not always properly distinguished in their isotopic record, in both ancient and modern settings. It is therefore crucial to refine our understanding of the processes controlling microbialites isotopic signal. Here, we analyzed the carbon and oxygen isotope compositions from bulk and micro-drilled carbonates as well as bulk organic carbon isotope compositions in microbialites from four Mexican volcanic crater lakes of increasing alkalinity. The survey of four lakes allows comparing microbialite formation processes and their geochemical record within distinct physico-chemical contexts. The geochemical analyses were performed in parallel to petrographic and mineralogical characterization and interpreted in light of the known microbial community composition for microbialites of the same lakes. Combining these data, we show that the potential for isotopic biosignature preservation primarily depends on physico-chemical conditions. Carbon isotope biosignatures pointing out to an autotrophic influence on carbonate precipitation are preserved in the lowest alkalinity lakes. By contrast, higher alkalinity lakes, where microbialites are more massive, favor carbonate precipitation in isotopic equilibrium with the lake water, with secondary influence of heterotrophic organic carbon degradation. From these results, we suggest that microbialite carbonate C isotope records can be interpreted as the balance between the microbialite net primary productivity and the amount of precipitation that relates to physico-chemical forcing. The signals of microbialite oxygen isotope compositions highlight a lack of understanding in the oxygen isotope records of relatively rare carbonate phases such as hydromagnesite. Nonetheless, we show that these signals are primarily influenced by the basins' hydrology, though biological effects may also play a (minor) role. Overall, both carbon and oxygen isotopic signals may record a mixture of different local/global and biotic/abiotic phenomena, making microbialites intricate archives of their growth environment, which should thus be interpreted with cautions and in the light of their surrounding sediments.