Journal of thermal biology最新文献_第10页

Influence of temperature on early life stages of silver pompano, Trachinotus blochii 温度对银鲳鱼生命早期阶段的影响。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-12 DOI: 10.1016/j.jtherbio.2025.104328

Krishna Sukumaran , P.S. Pooja , Ambarish P. Gop , Anuraj Anirudhan , G. Rathi Bhuvaneswari , Archana Satheesh , Liyakhath Ali , Muhammed Arshadudeen , S. Nisha , A.P. Dineshbabu , B. Santhosh , V.V.R. Suresh , A. Gopalakrishnan , Grinson George

Silver pompano, Trachinotus blochii is an important mariculture species in India and Southeast Asia. Understanding the influence of temperature, the most critical environmental factor on the early life stages is crucial for identifying the optimal rearing temperature for its hatchery production and assessing the species resilience to climate change. This study evaluated embryogenesis, hatching, yolk-sac larval development and survival at four environmental temperatures, 24 ± 0.5 °C, 27 ± 0.5 °C, 30 ± 0.5 °C and 33 ± 0.5 °C in thermostatically controlled 50-L tanks (in triplicates). It was found that higher temperatures significantly (P < 0.05) accelerated embryogenesis and the shortest developmental time was recorded at 33 °C (14.5 ± 2.6 h). A significantly higher hatching rate (P < 0.05) was recorded at 30 °C, this was accompanied by a significantly (P < 0.05) reduced hatching duration at the higher temperatures. The larvae that hatched at 24 °C had highest total length whereas the total larval length recorded at onset of exogenous feeding was significantly (P < 0.05) higher at 33 °C. The larval activity, utilisation of endogenous energy reserves (yolk sac volume and oil globule) was also significantly (P < 0.05) higher at 30 °C and 33 °C. Although some embryonic and larval development parameters were comparable at 30 °C and 33 °C, there was a significant incidence of morphological abnormalities (10.00 ± 4.08 %), reduction in hatching % and survival of yolk-sac larvae at 33 °C. These results indicate that 30 ± 0.5 °C is the optimal thermal environment for rearing of early stages of silver pompano as it minimises developmental abnormalities, optimises survival and enhances the efficiency of its hatchery production.

银鲳（Trachinotus blochii）是印度和东南亚重要的海水养殖品种。了解温度这一最关键的环境因子对其生命早期阶段的影响，对于确定其孵化场生产的最佳饲养温度和评估物种对气候变化的适应能力至关重要。本研究在恒温控制的50-L培养皿中（3个），分别在24±0.5°C、27±0.5°C、30±0.5°C和33±0.5°C四种环境温度下，评估了胚胎发生、孵化、卵囊幼虫的发育和存活率。结果表明，较高的温度显著（P < 0.05）促进了胚胎的发生，33℃时发育时间最短（14.5±2.6 h）。30°C条件下的孵化率显著高于（P < 0.05），高温条件下的孵化时间显著缩短（P < 0.05）。在24°C时孵化的幼虫总长度最高，而在33°C时孵化的幼虫总长度显著（P < 0.05）高于24°C。在30°C和33°C条件下，幼虫活力、内源能量储备（卵黄囊体积和油球）利用率也显著（P < 0.05）提高。虽然在30°C和33°C下，一些胚胎和幼虫的发育参数相当，但在33°C下，卵黄囊幼虫的形态异常发生率显著（10.00±4.08%），孵化率和存活率降低。以上结果表明，30±0.5°C是早期鲳鱼养殖的最佳温度环境，可以最大限度地减少发育异常，优化成活率，提高孵化场生产效率。

{"title":"Influence of temperature on early life stages of silver pompano, Trachinotus blochii","authors":"Krishna Sukumaran , P.S. Pooja , Ambarish P. Gop , Anuraj Anirudhan , G. Rathi Bhuvaneswari , Archana Satheesh , Liyakhath Ali , Muhammed Arshadudeen , S. Nisha , A.P. Dineshbabu , B. Santhosh , V.V.R. Suresh , A. Gopalakrishnan , Grinson George","doi":"10.1016/j.jtherbio.2025.104328","DOIUrl":"10.1016/j.jtherbio.2025.104328","url":null,"abstract":"<div><div>Silver pompano, <em>Trachinotus blochii</em> is an important mariculture species in India and Southeast Asia. Understanding the influence of temperature, the most critical environmental factor on the early life stages is crucial for identifying the optimal rearing temperature for its hatchery production and assessing the species resilience to climate change. This study evaluated embryogenesis, hatching, yolk-sac larval development and survival at four environmental temperatures, 24 ± 0.5 °C, 27 ± 0.5 °C, 30 ± 0.5 °C and 33 ± 0.5 °C in thermostatically controlled 50-L tanks (in triplicates). It was found that higher temperatures significantly (<em>P <</em> 0.05) accelerated embryogenesis and the shortest developmental time was recorded at 33 °C (14.5 ± 2.6 h). A significantly higher hatching rate (<em>P <</em> 0.05) was recorded at 30 °C, this was accompanied by a significantly (<em>P <</em> 0.05) reduced hatching duration at the higher temperatures. The larvae that hatched at 24 °C had highest total length whereas the total larval length recorded at onset of exogenous feeding was significantly (<em>P <</em> 0.05) higher at 33 °C. The larval activity, utilisation of endogenous energy reserves (yolk sac volume and oil globule) was also significantly (<em>P <</em> 0.05) higher at 30 °C and 33 °C. Although some embryonic and larval development parameters were comparable at 30 °C and 33 °C, there was a significant incidence of morphological abnormalities (10.00 ± 4.08 %), reduction in hatching % and survival of yolk-sac larvae at 33 °C. These results indicate that 30 ± 0.5 °C is the optimal thermal environment for rearing of early stages of silver pompano as it minimises developmental abnormalities, optimises survival and enhances the efficiency of its hatchery production.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104328"},"PeriodicalIF":2.9,"publicationDate":"2025-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145564410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modelling the pipping to emergence interval and its influence on the thermosensitive period for sex determination in the green sea turtle Chelonia mydas 绿海龟羽化期的建模及其对性别决定温敏期的影响

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-12 DOI: 10.1016/j.jtherbio.2025.104320

Kirsty Scott , Jesse E.M. Cochran , Royale S. Hardenstine , Marc Girondot , Michael L. Berumen

Sea turtle survival rate and hatchling sex ratios are closely linked to incubation temperature and duration, both of which are critical for understanding species resilience to climate change. This is particularly important for sea turtles currently nesting in thermally stressed environments, such as the North West Indian Ocean green sea turtle population. However, there is a methodological discrepancy between field studies (which usually measure incubation duration from oviposition to emergence) and constant temperature incubation studies (which measure from oviposition to either pipping or hatching time). The period between hatching and emergence can span several days, potentially affecting estimates of the thermosensitive period (TSP) when sex is determined. This study investigates the often-overlooked interval between hatching and emergence, a developmental phase essential in hatchling survival. We evaluate two non-invasive methods to estimate this interval: (1) deploying tri-axial accelerometers and temperature loggers to detect movement associated with hatching, and (2) comparing laboratory and field incubation durations using fitted models. Results reveal that field-based durations consistently exceed laboratory-based predictions, primarily due to the inclusion of the emergence to pipping time. Across seven nests, using emergence instead of pipping to mark the end of incubation raised mean TSP temperature by 0.1–0.5 °C and lengthened the inferred TSP by about four days. Our findings highlight the implications of this discrepancy for modelling TSP timing; by excluding the pipping to emergence time. While this bias may seem minor, the narrow thermal range of sex determination means it could significantly skew sex ratios and lead to artificial feminisation estimations. By refining incubation duration metrics, this work enhances the accuracy of sex ratio predictions and provides new insights into the hidden pipping dynamics informing conservation strategies such as beach nourishment and hatchery practices.

海龟的存活率和孵化性别比与孵化温度和持续时间密切相关，这两者对于了解物种对气候变化的适应能力至关重要。这对目前在高温环境中筑巢的海龟尤其重要，比如西北印度洋的绿海龟。然而，实地研究（通常测量从产卵到羽化的孵化时间）和恒温孵化研究（测量从产卵到抽芽或孵化时间）在方法上存在差异。孵化和羽化之间的时间可能长达数天，这可能会影响到确定性别时对热敏期（TSP）的估计。本研究调查了经常被忽视的孵化和羽化之间的间隔，这是一个对孵化生存至关重要的发育阶段。我们评估了两种非侵入性方法来估计这个间隔：(1)部署三轴加速度计和温度记录仪来检测与孵化相关的运动，(2)使用拟合模型比较实验室和现场孵化持续时间。结果表明，基于现场的持续时间始终超过基于实验室的预测，主要是由于包含了出现到管道时间。在7个巢穴中，使用羽化而不是羽化来标记孵化结束，将平均TSP温度提高了0.1-0.5°C，并将推断的TSP延长了约4天。我们的研究结果强调了这种差异对TSP时间建模的影响；通过排除出现时间的跳动。虽然这种偏差可能看起来很小，但性别决定的狭窄热范围意味着它可能会严重扭曲性别比例，并导致人为的女性化估计。通过改进孵化时间指标，这项工作提高了性别比例预测的准确性，并为隐藏的管道动态提供了新的见解，为保护策略（如海滩营养和孵化场实践）提供了信息。

{"title":"Modelling the pipping to emergence interval and its influence on the thermosensitive period for sex determination in the green sea turtle Chelonia mydas","authors":"Kirsty Scott , Jesse E.M. Cochran , Royale S. Hardenstine , Marc Girondot , Michael L. Berumen","doi":"10.1016/j.jtherbio.2025.104320","DOIUrl":"10.1016/j.jtherbio.2025.104320","url":null,"abstract":"<div><div>Sea turtle survival rate and hatchling sex ratios are closely linked to incubation temperature and duration, both of which are critical for understanding species resilience to climate change. This is particularly important for sea turtles currently nesting in thermally stressed environments, such as the North West Indian Ocean green sea turtle population. However, there is a methodological discrepancy between field studies (which usually measure incubation duration from oviposition to emergence) and constant temperature incubation studies (which measure from oviposition to either pipping or hatching time). The period between hatching and emergence can span several days, potentially affecting estimates of the thermosensitive period (TSP) when sex is determined. This study investigates the often-overlooked interval between hatching and emergence, a developmental phase essential in hatchling survival. We evaluate two non-invasive methods to estimate this interval: (1) deploying tri-axial accelerometers and temperature loggers to detect movement associated with hatching, and (2) comparing laboratory and field incubation durations using fitted models. Results reveal that field-based durations consistently exceed laboratory-based predictions, primarily due to the inclusion of the emergence to pipping time. Across seven nests, using emergence instead of pipping to mark the end of incubation raised mean TSP temperature by 0.1–0.5 °C and lengthened the inferred TSP by about four days. Our findings highlight the implications of this discrepancy for modelling TSP timing; by excluding the pipping to emergence time. While this bias may seem minor, the narrow thermal range of sex determination means it could significantly skew sex ratios and lead to artificial feminisation estimations. By refining incubation duration metrics, this work enhances the accuracy of sex ratio predictions and provides new insights into the hidden pipping dynamics informing conservation strategies such as beach nourishment and hatchery practices.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104320"},"PeriodicalIF":2.9,"publicationDate":"2025-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145570017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermosensitivity of the Microvasculature: Molecular and physiological mechanisms in skeletal muscle – A narrative review 微血管的热敏性：骨骼肌的分子和生理机制综述

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-08 DOI: 10.1016/j.jtherbio.2025.104331

Sean P. Langan , Douglas J. Casa , Oh Sung Kwon

Chronic heat stress is a potent intervention to improve vascular health, specifically within the cutaneous and skeletal muscle microcirculation. While the role of cutaneous circulation in heat dissipation is well established, emerging evidence suggests that skeletal muscle vasculature also exhibits thermosensitivity, contributing to heat-induced hyperemia beyond metabolic demand. This narrative review explores the molecular and physiological mechanisms underlying thermal hyperemia, focusing on heat-sensitive ion channels (TRPV1, TRPV4), nitric oxide (NO) signaling, and endothelial adaptations. The role of exercise-induced heat production and heat acclimation in promoting vascular plasticity is also examined, with particular emphasis on vascular remodeling and microcirculatory adaptations. Furthermore, this review highlights the pathological consequences of excessive heat exposure, including heat stroke, endothelial dysfunction, and systemic inflammation, emphasizing the delicate balance between adaptive and maladaptive vascular responses to heat stress. Understanding the interplay between heat stress, vascular regulation, and exercise adaptation provides valuable insights into potential therapeutic applications of heat acclimation for individuals with cardiovascular disease, metabolic disorders, and impaired thermoregulation.

慢性热应激是改善血管健康的有效干预措施，特别是在皮肤和骨骼肌微循环中。虽然皮肤循环在散热中的作用已经确立，但新出现的证据表明，骨骼肌血管系统也表现出热敏性，导致热致充血超出代谢需求。本文探讨了热充血的分子和生理机制，重点关注热敏离子通道（TRPV1, TRPV4），一氧化氮（NO）信号传导和内皮细胞适应。运动诱导的产热和热适应在促进血管可塑性中的作用也被检查，特别强调血管重塑和微循环适应。此外，这篇综述强调了过度热暴露的病理后果，包括中暑、内皮功能障碍和全身性炎症，强调了血管对热应激的适应性和非适应性反应之间的微妙平衡。了解热应激、血管调节和运动适应之间的相互作用，为热适应对心血管疾病、代谢紊乱和体温调节受损个体的潜在治疗应用提供了有价值的见解。

{"title":"Thermosensitivity of the Microvasculature: Molecular and physiological mechanisms in skeletal muscle – A narrative review","authors":"Sean P. Langan , Douglas J. Casa , Oh Sung Kwon","doi":"10.1016/j.jtherbio.2025.104331","DOIUrl":"10.1016/j.jtherbio.2025.104331","url":null,"abstract":"<div><div>Chronic heat stress is a potent intervention to improve vascular health, specifically within the cutaneous and skeletal muscle microcirculation. While the role of cutaneous circulation in heat dissipation is well established, emerging evidence suggests that skeletal muscle vasculature also exhibits thermosensitivity, contributing to heat-induced hyperemia beyond metabolic demand. This narrative review explores the molecular and physiological mechanisms underlying thermal hyperemia, focusing on heat-sensitive ion channels (TRPV1, TRPV4), nitric oxide (NO) signaling, and endothelial adaptations. The role of exercise-induced heat production and heat acclimation in promoting vascular plasticity is also examined, with particular emphasis on vascular remodeling and microcirculatory adaptations. Furthermore, this review highlights the pathological consequences of excessive heat exposure, including heat stroke, endothelial dysfunction, and systemic inflammation, emphasizing the delicate balance between adaptive and maladaptive vascular responses to heat stress. Understanding the interplay between heat stress, vascular regulation, and exercise adaptation provides valuable insights into potential therapeutic applications of heat acclimation for individuals with cardiovascular disease, metabolic disorders, and impaired thermoregulation.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104331"},"PeriodicalIF":2.9,"publicationDate":"2025-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145518698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling bio-climatic thermal stress driven behavioral pattern shifts in crossbred pigs 揭示生物气候热应激驱动杂交猪的行为模式转变。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-08 DOI: 10.1016/j.jtherbio.2025.104332

Ajay Argana , Ayon Tarafdar , Sakshi Vaishnav , Gyanendra Kumar Gaur , Mukesh Singh , CK Faslu Rahman , Gyanendra Singh , Rupasi Tiwari , Triveni Dutt , Anuj Chauhan

Pigs are highly susceptible to heat stress and their performance affects sustainability of the pig farming sector. It is therefore, essential to understand their behavior in different climatic conditions. In this work, 24 Landlly crossbred pigs in grower stage with uniform body weight were selected and randomly divided into two groups (heat stress [HS] and heat stress alleviated [HSA]). The HSA group was supplemented with a sensor-based cooling system to minimize heat stress. Major micro-climatic variables viz., ambient temperature (AT), relative humidity (RH %) and temperature humidity index (THI) were recorded twice daily in both pens. Mean AT, RH and THI around the micro-climate were 29.6 ± 0.15 °C, 72.4 ± 0.19 % and 80 ± 0.24, respectively. The lying, sitting, standing, feeding, drinking, and agonistic behavior were recorded daily for 90 days (June, July, August 2023) using 2 fixed Axis M1065 IP cameras. Ethogram was prepared for each behavior individually after segregating the recording from 0900 to 1700 h where maximum AT, RH and THI variations were experienced. Significant increase in the duration of lateral lying, water trough access, walking and standing posture (p < 0.01) were observed in heat stressed pigs. Average feed intake time per animal increased by ∼15 % than normal. During extreme periods of heat, time spend for sternal lying and sitting was significantly lower by 55 % and 25 %, respectively (p < 0.01). Additionally, high incidence of tail biting and agonistic behaviors (p < 0.01) were observed in the HS group. Variation within groups revealed that harsh effects of heat stress in pigs were more evident during the hot-dry period.

猪对热应激非常敏感，它们的性能影响着养猪业的可持续性。因此，有必要了解它们在不同气候条件下的行为。选取24头体重均匀的生长期Landlly杂交猪，随机分为热应激组（HS）和热应激缓解组（HSA）。HSA组补充了一个基于传感器的冷却系统，以尽量减少热应力。主要的微气候变量，即环境温度（AT）、相对湿度（RH %）和温度湿度指数（THI），在两个围栏中每天记录两次。小气候周围的平均AT、RH和THI分别为29.6±0.15°C、72.4±0.19%和80±0.24%。使用2台固定Axis M1065 IP摄像机，每天记录90天（2023年6月、7月、8月）小鼠的卧、坐、站、摄食、饮水和激动行为。从0900到1700h，在经历最大的AT、RH和THI变化后，分离记录，分别为每种行为准备心电图。侧卧、取水、行走和站立姿势的持续时间显著增加

{"title":"Unravelling bio-climatic thermal stress driven behavioral pattern shifts in crossbred pigs","authors":"Ajay Argana , Ayon Tarafdar , Sakshi Vaishnav , Gyanendra Kumar Gaur , Mukesh Singh , CK Faslu Rahman , Gyanendra Singh , Rupasi Tiwari , Triveni Dutt , Anuj Chauhan","doi":"10.1016/j.jtherbio.2025.104332","DOIUrl":"10.1016/j.jtherbio.2025.104332","url":null,"abstract":"<div><div>Pigs are highly susceptible to heat stress and their performance affects sustainability of the pig farming sector. It is therefore, essential to understand their behavior in different climatic conditions. In this work, 24 Landlly crossbred pigs in grower stage with uniform body weight were selected and randomly divided into two groups (heat stress [HS] and heat stress alleviated [HSA]). The HSA group was supplemented with a sensor-based cooling system to minimize heat stress. Major micro-climatic variables <em>viz.</em>, ambient temperature (AT), relative humidity (RH %) and temperature humidity index (THI) were recorded twice daily in both pens. Mean AT, RH and THI around the micro-climate were 29.6 ± 0.15 °C, 72.4 ± 0.19 % and 80 ± 0.24, respectively. The lying, sitting, standing, feeding, drinking, and agonistic behavior were recorded daily for 90 days (June, July, August 2023) using 2 fixed Axis M1065 IP cameras. Ethogram was prepared for each behavior individually after segregating the recording from 0900 to 1700 h where maximum AT, RH and THI variations were experienced. Significant increase in the duration of lateral lying, water trough access, walking and standing posture (p < 0.01) were observed in heat stressed pigs. Average feed intake time per animal increased by ∼15 % than normal. During extreme periods of heat, time spend for sternal lying and sitting was significantly lower by 55 % and 25 %, respectively (p < 0.01). Additionally, high incidence of tail biting and agonistic behaviors (p < 0.01) were observed in the HS group. Variation within groups revealed that harsh effects of heat stress in pigs were more evident during the hot-dry period.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104332"},"PeriodicalIF":2.9,"publicationDate":"2025-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145489115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosis of superficial ailments using infrared thermal imaging and CapsNet 应用红外热成像和CapsNet诊断浅表疾病。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-07 DOI: 10.1016/j.jtherbio.2025.104325

Bhaskar Pandey , Jaspreet Singh , Deepak Joshi , Shiv Ram Dubey , Ajat Shatru Arora

Infrared thermal imaging has been widely recognized as a reliable tool for detecting superficial diseases by analyzing temperature variations. However, external factors can influence regional temperature, introducing potential errors in diagnosis. This study investigates the integration of a computer vision-based classification algorithm with thermal imaging to enhance disease classification and diagnosis. Specifically, the research explores the application of Capsule Networks (CapsNet) for diagnosing conditions with skin-manifesting symptoms, such as breast cancer, pressure ulcers, and sinusitis. The proposed approach involves processing thermal images as conventional images using a computer vision-based classification algorithm. CapsNet is employed as the primary deep learning model for disease classification. The performance of CapsNet is evaluated and compared against benchmark models to assess its effectiveness in diagnosing breast cancer, pressure ulcers, and sinusitis. Experimental results demonstrate that CapsNet consistently outperforms traditional models across all evaluated conditions. The model achieves an accuracy of 99.91 % in breast cancer detection, 92.12 % in pressure ulcer staging, and 99.10 % and 98.93 % in multiclass and binary sinusitis classification, respectively. The integration of thermal imaging with CapsNet presents a promising approach for diagnosing superficial diseases. The high accuracy rates across different medical conditions highlight the potential of this method in medical imaging applications. These findings suggest that thermal imaging, when combined with advanced deep learning techniques, should be more widely adopted in medical diagnostics.

红外热成像已被广泛认为是通过分析温度变化来检测浅表疾病的可靠工具。然而，外部因素可能会影响区域温度，从而导致潜在的诊断错误。本研究将基于计算机视觉的分类算法与热成像相结合，以增强疾病的分类和诊断。具体而言，该研究探讨了胶囊网络（CapsNet）在诊断具有皮肤症状的疾病方面的应用，如乳腺癌、压疮和鼻窦炎。所提出的方法包括使用基于计算机视觉的分类算法将热图像作为常规图像处理。采用CapsNet作为疾病分类的主要深度学习模型。对CapsNet的性能进行评估，并与基准模型进行比较，以评估其在诊断乳腺癌、压疮和鼻窦炎方面的有效性。实验结果表明，CapsNet在所有评估条件下都优于传统模型。该模型对乳腺癌的检测准确率为99.91%，对压疮分期准确率为92.12%，对多分型和二元鼻窦炎分期准确率分别为99.10%和98.93%。热成像与CapsNet的结合为浅表疾病的诊断提供了一种很有前途的方法。在不同医疗条件下的高准确率突出了该方法在医学成像应用中的潜力。这些发现表明，热成像与先进的深度学习技术相结合，应该更广泛地应用于医学诊断。

{"title":"Diagnosis of superficial ailments using infrared thermal imaging and CapsNet","authors":"Bhaskar Pandey , Jaspreet Singh , Deepak Joshi , Shiv Ram Dubey , Ajat Shatru Arora","doi":"10.1016/j.jtherbio.2025.104325","DOIUrl":"10.1016/j.jtherbio.2025.104325","url":null,"abstract":"<div><div>Infrared thermal imaging has been widely recognized as a reliable tool for detecting superficial diseases by analyzing temperature variations. However, external factors can influence regional temperature, introducing potential errors in diagnosis. This study investigates the integration of a computer vision-based classification algorithm with thermal imaging to enhance disease classification and diagnosis. Specifically, the research explores the application of Capsule Networks (CapsNet) for diagnosing conditions with skin-manifesting symptoms, such as breast cancer, pressure ulcers, and sinusitis. The proposed approach involves processing thermal images as conventional images using a computer vision-based classification algorithm. CapsNet is employed as the primary deep learning model for disease classification. The performance of CapsNet is evaluated and compared against benchmark models to assess its effectiveness in diagnosing breast cancer, pressure ulcers, and sinusitis. Experimental results demonstrate that CapsNet consistently outperforms traditional models across all evaluated conditions. The model achieves an accuracy of 99.91 % in breast cancer detection, 92.12 % in pressure ulcer staging, and 99.10 % and 98.93 % in multiclass and binary sinusitis classification, respectively. The integration of thermal imaging with CapsNet presents a promising approach for diagnosing superficial diseases. The high accuracy rates across different medical conditions highlight the potential of this method in medical imaging applications. These findings suggest that thermal imaging, when combined with advanced deep learning techniques, should be more widely adopted in medical diagnostics.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104325"},"PeriodicalIF":2.9,"publicationDate":"2025-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145505177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms underlying population-specific thermal stress responses in the rice-paddy frog (Fejervarya multistriata) 水稻蛙（Fejervarya multistriata）群体特异性热应激反应的分子机制。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-06 DOI: 10.1016/j.jtherbio.2025.104314

Xiao-Dong Xu , Ling-Na Cai , Kenneth B. Storey , Jia-Yong Zhang , Dan-Na Yu

Understanding how species adapt to environmental stress is essential for predicting their resilience to climate change. As ectotherms, amphibians are particularly susceptible to temperature fluctuations. Here, we analyzed liver transcriptomes from 18 individual frogs of Fejervarya multistriata (3 biological replicates × 3 treatments × 2 populations: Guilin, Guangxi, with relatively stable warm temperatures throughout the year, and Jinhua, Zhejiang, with more variable seasonal temperatures) exposed to low (4 °C), high (40 °C), or control (25 °C) conditions. Comparative analyses revealed divergent molecular responses between populations. Guilin frogs exhibited extensive transcriptional shifts (1469 up/1627 down under heat; 804 up/1553 down under cold) enriched for energy metabolism, particularly oxidative phosphorylation (OXPHOS). By contrast, Jinhua frogs showed far fewer DEGs (234 up/267 down under heat; 54 up/13 down under cold), indicating reduced transcriptional plasticity. Taken together, these results demonstrate pronounced population-level differences in transcriptomic responses to thermal stress that are consistent with local climatic regimes. The stronger OXPHOS-associated signature in Guilin suggests enhanced expression-level metabolic flexibility during both heat and cold, whereas the muted response in Jinhua indicates a more conservative strategy. We highlight OXPHOS transcription as a candidate indicator of thermal resilience pending physiological validation, underscoring the value of integrating intraspecific transcriptomic variability into conservation planning under rapid warming.

了解物种如何适应环境压力对于预测它们对气候变化的适应能力至关重要。作为变温动物，两栖动物特别容易受到温度波动的影响。本研究分析了18只多纹Fejervarya multistriata蛙（3个生物重复× 3个处理× 2个种群：广西桂林全年相对稳定的温暖环境和浙江金华季节温度变化较大的环境）暴露在低（4℃）、高（40℃）或对照（25℃）条件下的肝脏转录组。比较分析揭示了不同种群间不同的分子反应。桂林蛙表现出广泛的转录变化（高温下向上1469 /向下1627；低温下向上804 /向下1553），有利于能量代谢，特别是氧化磷酸化（OXPHOS）。相比之下，金华蛙在高温条件下的温度升高（234 /267），低温条件下的温度升高（54 /13），表明转录可塑性降低。综上所述，这些结果表明，不同种群对热应激的转录组反应存在显著差异，这与当地气候条件一致。桂林的oxphos相关信号较强，表明在冷热环境下表达水平的代谢灵活性增强，而金华的反应较弱，表明其代谢策略更为保守。我们强调了OXPHOS转录作为热恢复能力的候选指标，有待生理学验证，强调了将种内转录组变异性整合到快速变暖下的保护规划中的价值。

{"title":"Molecular mechanisms underlying population-specific thermal stress responses in the rice-paddy frog (Fejervarya multistriata)","authors":"Xiao-Dong Xu , Ling-Na Cai , Kenneth B. Storey , Jia-Yong Zhang , Dan-Na Yu","doi":"10.1016/j.jtherbio.2025.104314","DOIUrl":"10.1016/j.jtherbio.2025.104314","url":null,"abstract":"<div><div>Understanding how species adapt to environmental stress is essential for predicting their resilience to climate change. As ectotherms, amphibians are particularly susceptible to temperature fluctuations. Here, we analyzed liver transcriptomes from 18 individual frogs of <em>Fejervarya multistriata</em> (3 biological replicates × 3 treatments × 2 populations: Guilin, Guangxi, with relatively stable warm temperatures throughout the year, and Jinhua, Zhejiang, with more variable seasonal temperatures) exposed to low (4 °C), high (40 °C), or control (25 °C) conditions. Comparative analyses revealed divergent molecular responses between populations. Guilin frogs exhibited extensive transcriptional shifts (1469 up/1627 down under heat; 804 up/1553 down under cold) enriched for energy metabolism, particularly oxidative phosphorylation (OXPHOS). By contrast, Jinhua frogs showed far fewer DEGs (234 up/267 down under heat; 54 up/13 down under cold), indicating reduced transcriptional plasticity. Taken together, these results demonstrate pronounced population-level differences in transcriptomic responses to thermal stress that are consistent with local climatic regimes. The stronger OXPHOS-associated signature in Guilin suggests enhanced expression-level metabolic flexibility during both heat and cold, whereas the muted response in Jinhua indicates a more conservative strategy. We highlight OXPHOS transcription as a candidate indicator of thermal resilience pending physiological validation, underscoring the value of integrating intraspecific transcriptomic variability into conservation planning under rapid warming.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104314"},"PeriodicalIF":2.9,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145505170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of hyperthermia in avian tolerance of hot and humid conditions 热疗在鸟类对湿热环境的耐受性中的作用。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-06 DOI: 10.1016/j.jtherbio.2025.104324

Marc T. Freeman , Bianca Coulson , Celiwe A. Ngcamphalala , Mathome O. Makola , Andrew E. McKechnie

Pronounced hyperthermia is thought to be important for thermoregulation during hot weather in animals inhabiting humid environments, where high atmospheric humidity constrains evaporative cooling. To assess the role of facultative, reversible hyperthermia in avian thermoregulation under hot, humid conditions, we examined how humidity influences thermoregulatory performance at air temperatures (T_air) exceeding normothermic body temperature (T_b) in red-billed queleas (Quelea quelea), a species capable of extreme hyperthermia (T_b > 48 °C). Using flow-through respirometry, we exposed queleas to T_air = 34 °C–50 °C at four experimental absolute humidities (6 g H₂O m^{− 3}, 13 g H₂O m^{− 3}, 19 g H₂O m^{− 3}, and 25 g H₂O m⁻³). Across these humidity treatments, resting metabolic rate, evaporative water loss and maximum evaporative cooling efficiency responded similarly to increasing T_air, and maximum tolerable body temperature (T_bmax) remained consistent (48.60 ± 0.40 °C). Heat tolerance limits (HTL; environmental temperatures associated with thermoregulatory failure) averaged T_air = 48.41 ± 1.03 °C among humidity treatments. At T_air > ∼44.5 °C, evaporative cooling efficiency plateaued and queleas became poikilothermic, with T_b tracking T_air ≤ ∼48.4 °C. These findings demonstrate extreme hyperthermia tolerance can offset the constraints on evaporative cooling imposed by high humidity, allowing some birds to tolerate extreme combinations of heat and humidity. We propose a modification to an existing conceptual framework of the thermoregulatory roles of facultative hyperthermia to accommodate the effects of humidity on evaporative cooling and heat tolerance limits.

高温被认为对生活在潮湿环境中的动物在炎热天气中的体温调节很重要，因为高大气湿度限制了蒸发冷却。为了评估在炎热潮湿条件下，兼性可逆热疗在鸟类体温调节中的作用，我们研究了在空气温度（Tair）超过恒温体温（Tb）时，湿度如何影响红嘴魁利亚（Quelea Quelea）的体温调节性能，红嘴魁利亚是一种能够极端热疗（Tb bbb48°C）的物种。使用流式呼吸法，我们将queleas暴露在Tair = 34°C-50°C的四种实验绝对湿度（6 g H2O m-3, 13 g H2O m-3, 19 g H2O m-3和25 g H2O m-3）下。在这些湿度处理中，静息代谢率、蒸发失水和最大蒸发冷却效率对Tair的增加有相似的反应，最大可耐受体温（Tbmax）保持一致（48.60±0.40°C）。湿度处理的耐热极限（HTL；与温度调节失效相关的环境温度）平均Tair = 48.41±1.03°C。在Tair≤44.5°C时，蒸发冷却效率趋于平稳，queleas变为变热，Tb跟踪Tair≤~ 48.4°C。这些发现表明，极端的高温耐受性可以抵消高湿度对蒸发冷却的限制，使一些鸟类能够忍受极端的高温和湿度组合。我们建议对兼性热疗的热调节作用的现有概念框架进行修改，以适应湿度对蒸发冷却和耐热极限的影响。

{"title":"The role of hyperthermia in avian tolerance of hot and humid conditions","authors":"Marc T. Freeman , Bianca Coulson , Celiwe A. Ngcamphalala , Mathome O. Makola , Andrew E. McKechnie","doi":"10.1016/j.jtherbio.2025.104324","DOIUrl":"10.1016/j.jtherbio.2025.104324","url":null,"abstract":"<div><div>Pronounced hyperthermia is thought to be important for thermoregulation during hot weather in animals inhabiting humid environments, where high atmospheric humidity constrains evaporative cooling. To assess the role of facultative, reversible hyperthermia in avian thermoregulation under hot, humid conditions, we examined how humidity influences thermoregulatory performance at air temperatures (<em>T</em><sub>air</sub>) exceeding normothermic body temperature (<em>T</em><sub>b</sub>) in red-billed queleas (<em>Quelea quelea</em>), a species capable of extreme hyperthermia (<em>T</em><sub>b</sub> > 48 °C). Using flow-through respirometry, we exposed queleas to <em>T</em><sub>air</sub> = 34 °C–50 °C at four experimental absolute humidities (6 g H<sub>2</sub>O m<sup>− 3</sup>, 13 g H<sub>2</sub>O m<sup>− 3</sup>, 19 g H<sub>2</sub>O m<sup>− 3</sup>, and 25 g H<sub>2</sub>O m<sup>−3</sup>). Across these humidity treatments, resting metabolic rate, evaporative water loss and maximum evaporative cooling efficiency responded similarly to increasing <em>T</em><sub>air</sub>, and maximum tolerable body temperature (<em>T</em><sub>b</sub><em>max</em>) remained consistent (48.60 ± 0.40 °C). Heat tolerance limits (HTL; environmental temperatures associated with thermoregulatory failure) averaged <em>T</em><sub>air</sub> = 48.41 ± 1.03 °C among humidity treatments. At <em>T</em><sub>air</sub> > ∼44.5 °C, evaporative cooling efficiency plateaued and queleas became poikilothermic, with <em>T</em><sub>b</sub> tracking <em>T</em><sub>air</sub> ≤ ∼48.4 °C. These findings demonstrate extreme hyperthermia tolerance can offset the constraints on evaporative cooling imposed by high humidity, allowing some birds to tolerate extreme combinations of heat and humidity. We propose a modification to an existing conceptual framework of the thermoregulatory roles of facultative hyperthermia to accommodate the effects of humidity on evaporative cooling and heat tolerance limits.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104324"},"PeriodicalIF":2.9,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145513165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Numerical study on thermo-mechanical interaction in skin tissue with temperature-dependent properties under sequential cold and heat stimulation 连续冷热刺激下具有温度依赖特性的皮肤组织热-力相互作用的数值研究。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-06 DOI: 10.1016/j.jtherbio.2025.104321

Yingze Wang, Zhaowei He, Sheng Zhang, Xiao li Dai

The combined application of pulsed laser irradiation and cryogen spray cooling (CSC) is a promising technique for treating deep skin lesions, such as port wine stains (PWS). However, optimizing this process requires a precise understanding of the dynamic thermo-mechanical responses in skin tissue under sequential cold and heat stimuli. This study presents a comprehensive bio-thermo-mechanical coupling model incorporating temperature-dependent physical properties and the dual-phase lag (DPL) bioheat transfer mechanism. The finite difference method is employed to numerically solve the governing equations, enabling the prediction of temperature, displacement, thermal stress, and thermal damage distributions in multi-layered skin tissue. Comparative analyses reveal the significant influence of temperature-dependent properties and laser parameters on the thermo-mechanical response. The results demonstrate that CSC effectively reduces epidermal thermal damage while inducing notable compressive stress, highlighting the need for balanced clinical protocols.

脉冲激光照射与冷冻剂喷雾冷却（CSC）联合应用是治疗葡萄酒斑（PWS）等深层皮肤病变的一种很有前途的技术。然而，优化这一过程需要精确理解在连续冷和热刺激下皮肤组织的动态热机械反应。本研究提出了一个综合的生物热-机械耦合模型，该模型结合了温度依赖的物理特性和双相滞后（DPL）生物传热机制。采用有限差分法对控制方程进行数值求解，实现了多层皮肤组织中温度、位移、热应力和热损伤分布的预测。对比分析表明，温度相关特性和激光参数对热-力学响应有显著影响。结果表明，CSC有效地减少了表皮热损伤，同时诱导了显著的压应力，强调了平衡临床方案的必要性。

{"title":"Numerical study on thermo-mechanical interaction in skin tissue with temperature-dependent properties under sequential cold and heat stimulation","authors":"Yingze Wang, Zhaowei He, Sheng Zhang, Xiao li Dai","doi":"10.1016/j.jtherbio.2025.104321","DOIUrl":"10.1016/j.jtherbio.2025.104321","url":null,"abstract":"<div><div>The combined application of pulsed laser irradiation and cryogen spray cooling (CSC) is a promising technique for treating deep skin lesions, such as port wine stains (PWS). However, optimizing this process requires a precise understanding of the dynamic thermo-mechanical responses in skin tissue under sequential cold and heat stimuli. This study presents a comprehensive bio-thermo-mechanical coupling model incorporating temperature-dependent physical properties and the dual-phase lag (DPL) bioheat transfer mechanism. The finite difference method is employed to numerically solve the governing equations, enabling the prediction of temperature, displacement, thermal stress, and thermal damage distributions in multi-layered skin tissue. Comparative analyses reveal the significant influence of temperature-dependent properties and laser parameters on the thermo-mechanical response. The results demonstrate that CSC effectively reduces epidermal thermal damage while inducing notable compressive stress, highlighting the need for balanced clinical protocols.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104321"},"PeriodicalIF":2.9,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145513227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for temporal effect of exposure to heat stress during various developmental stages on productive and reproductive performance as well as thermotolerance of reproductive performance in the offspring in dairy cows 不同发育阶段暴露于热应激对奶牛后代生产和繁殖性能以及繁殖性能耐热性影响的时间证据

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-06 DOI: 10.1016/j.jtherbio.2025.104326

Shayan Zarabadipour , Emadeddin Mobedi , Iman Allahyari , Faramarz Gharagozlou , Mehdi Vojgani , Reza Hemmati Baghbanani , Amirsina Akbarinejad , Vahid Akbarinejad

Prenatal and neonatal exposure to heat stress could negatively affect postnatal reproductive performance in cows. Yet regarding this effect, it has not been differentiated whether the cows were unexposed or exposed to heat stress during postnatal period.

Therefore, the present study investigated reproductive performance of offspring (n = 8774) conceiving in the first month of various seasons of year considering whether or not they were exposed to heat stress around their first life (nulliparous offspring) and postpartum (primiparous offspring) insemination. Developmental stages were assumed as preconceptional, first, second and third trimesters of fetal and neonatal periods.

The longest duration of exposure to heat stress was the second trimester of fetal period in spring group, the first trimester of fetal period in summer group, preconceptional and neonatal periods in fall group, and the third trimester of fetal period in winter group (P < 0.05). When exposure to heat stress did not occur around the first insemination, first service conception risk (FSCR) was the greatest, intermediate, and least in spring and summer, winter, and fall groups, respectively, in nulliparous and primiparous offspring (P < 0.05). Yet when exposure to heat stress occurred around the first insemination, FSCR was the least, intermediate, and greatest in spring and summer, winter, and fall groups, respectively, in nulliparous offspring. It was also less in spring and summer groups than fall and winter groups in primiparous offspring (P < 0.05).

In conclusion, the present study showed temporal developmental effects of heat stress not only on reproductive performance but also on thermotolerance of reproductive performance in the resultant progeny in cattle.

奶牛产前和新生儿暴露于热应激会对产后生殖性能产生负面影响。然而，关于这种影响，奶牛在产后是未暴露还是暴露于热应激中并没有被区分。因此，本研究考察了在不同季节的第一个月受孕的后代（n = 8774）的生殖性能，考虑了它们在第一生命（未生育后代）和产后（初产后代）授精时是否暴露于热应激。发育阶段假定为孕前，第一，第二和第三三个月的胎儿和新生儿期。热应激暴露时间最长的是春季组胎期中期、夏季组胎期中期、秋季组孕前、新生儿期和冬季组胎期晚期（P < 0.05）。首次授精前后未发生热应激时，春、夏、冬、秋3个组的首次服务受胎风险（FSCR）最高、中等、最低（P < 0.05）。然而，当首次授精前后暴露于热应激时，未生育子代的FSCR分别在春季、夏季、冬季和秋季组中最低、中等和最高。初生子代春、夏两组均低于秋、冬两组（P < 0.05）。综上所述，本研究表明，热应激不仅对繁殖性能有影响，而且对后代的耐热性和繁殖性能也有影响。

{"title":"Evidence for temporal effect of exposure to heat stress during various developmental stages on productive and reproductive performance as well as thermotolerance of reproductive performance in the offspring in dairy cows","authors":"Shayan Zarabadipour , Emadeddin Mobedi , Iman Allahyari , Faramarz Gharagozlou , Mehdi Vojgani , Reza Hemmati Baghbanani , Amirsina Akbarinejad , Vahid Akbarinejad","doi":"10.1016/j.jtherbio.2025.104326","DOIUrl":"10.1016/j.jtherbio.2025.104326","url":null,"abstract":"<div><div>Prenatal and neonatal exposure to heat stress could negatively affect postnatal reproductive performance in cows. Yet regarding this effect, it has not been differentiated whether the cows were unexposed or exposed to heat stress during postnatal period.</div><div>Therefore, the present study investigated reproductive performance of offspring (n = 8774) conceiving in the first month of various seasons of year considering whether or not they were exposed to heat stress around their first life (nulliparous offspring) and postpartum (primiparous offspring) insemination. Developmental stages were assumed as preconceptional, first, second and third trimesters of fetal and neonatal periods.</div><div>The longest duration of exposure to heat stress was the second trimester of fetal period in spring group, the first trimester of fetal period in summer group, preconceptional and neonatal periods in fall group, and the third trimester of fetal period in winter group (P < 0.05). When exposure to heat stress did not occur around the first insemination, first service conception risk (FSCR) was the greatest, intermediate, and least in spring and summer, winter, and fall groups, respectively, in nulliparous and primiparous offspring (P < 0.05). Yet when exposure to heat stress occurred around the first insemination, FSCR was the least, intermediate, and greatest in spring and summer, winter, and fall groups, respectively, in nulliparous offspring. It was also less in spring and summer groups than fall and winter groups in primiparous offspring (P < 0.05).</div><div>In conclusion, the present study showed temporal developmental effects of heat stress not only on reproductive performance but also on thermotolerance of reproductive performance in the resultant progeny in cattle.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104326"},"PeriodicalIF":2.9,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145468425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermotolerance and antiapoptosis enhancement by Lycium barbarum polysaccharide: counteracting mitochondrial oxidative stress in human sperm exposed to decabromodiphenyl ether (BDE-209) at 37 °C 枸杞多糖的耐热性和抗凋亡增强：在37℃暴露于十溴联苯醚（BDE-209）的人类精子中，抵消线粒体氧化应激。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-06 DOI: 10.1016/j.jtherbio.2025.104311

Pengyun Qiao , Le Li , Zilong Li, Feifan Sun, Fa Ren

Sperm damage resulting from exposure to environmental toxicants or elevated temperatures frequently involves overlapping mechanisms, most notably the induction of mitochondrial oxidative stress. Lycium barbarum polysaccharide (LBP), a bioactive dietary component, mitigates decabromodiphenyl ether (BDE-209)-induced human sperm toxicity by modulating oxidative stress and apoptosis. Exposure to environmentally relevant levels of BDE-209 (40–80 μmol/L) significantly impaired sperm quality, as evidenced by reduced motility, compromised membrane and acrosome integrity, and increased DNA fragmentation when compared to the control group at 37 °C. Mechanistically, exposure to BDE-209 induces mitochondrial oxidative stress, resulting in reactive oxygen species (ROS) accumulation, decreased mitochondrial membrane potential (MMP), and reduced ATP production. This is evidenced by increased activities of Caspase3 and Caspase9, elevated levels of BAX, and decreased expression of BCL-2. LBP intervention (100 μg/mL) effectively scavenged ROS, restored mitochondrial potential, and enhanced antioxidant capacity, as demonstrated by increased superoxide dismutase (SOD) and glutathione (GSH) levels, alongside decreased malondialdehyde (MDA) levels. Furthermore, LBP suppressed apoptotic pathways and enhanced sperm-zona pellucida binding after BDE-209 exposure. A proposed mechanism summarizing this protective pathway is presented in the Graphical Abstract. As a naturally occurring polysaccharide that protects against oxidative stress in sperm, LBP could represent a promising candidate for dietary interventions in populations exposed to elevated levels of BDE-209. This warrants further investigation in in vivo models.

暴露于环境有毒物质或高温导致的精子损伤通常涉及重叠机制，最明显的是线粒体氧化应激的诱导。枸杞多糖（LBP）是一种生物活性膳食成分，通过调节氧化应激和细胞凋亡，减轻十溴联苯醚（BDE-209）诱导的人类精子毒性。与对照组相比，暴露于环境相关水平的BDE-209 （40-80 μmol/L）显著损害精子质量，表现为活力降低，膜和顶体完整性受损，DNA断裂增加。从机制上说，暴露于BDE-209诱导线粒体氧化应激，导致活性氧（ROS）积累，降低线粒体膜电位（MMP），减少ATP的产生。Caspase3和Caspase9活性增加，BAX水平升高，BCL-2表达降低证明了这一点。LBP干预（100 μg/mL）可有效清除活性氧，恢复线粒体电位，增强抗氧化能力，结果表明，超氧化物歧化酶（SOD）和谷胱甘肽（GSH）水平升高，丙二醛（MDA）水平降低。此外，LBP可抑制BDE-209暴露后的凋亡通路，增强精子与透明带的结合。在图形摘要中提出了一种机制，总结了这种保护途径。作为一种天然存在的多糖，LBP可以防止精子中的氧化应激，它可能是BDE-209水平升高人群饮食干预的一个有希望的候选者。这值得在体内模型中进一步研究。

{"title":"Thermotolerance and antiapoptosis enhancement by Lycium barbarum polysaccharide: counteracting mitochondrial oxidative stress in human sperm exposed to decabromodiphenyl ether (BDE-209) at 37 °C","authors":"Pengyun Qiao , Le Li , Zilong Li, Feifan Sun, Fa Ren","doi":"10.1016/j.jtherbio.2025.104311","DOIUrl":"10.1016/j.jtherbio.2025.104311","url":null,"abstract":"<div><div>Sperm damage resulting from exposure to environmental toxicants or elevated temperatures frequently involves overlapping mechanisms, most notably the induction of mitochondrial oxidative stress. Lycium barbarum polysaccharide (LBP), a bioactive dietary component, mitigates decabromodiphenyl ether (BDE-209)-induced human sperm toxicity by modulating oxidative stress and apoptosis. Exposure to environmentally relevant levels of BDE-209 (40–80 μmol/L) significantly impaired sperm quality, as evidenced by reduced motility, compromised membrane and acrosome integrity, and increased DNA fragmentation when compared to the control group at 37 °C. Mechanistically, exposure to BDE-209 induces mitochondrial oxidative stress, resulting in reactive oxygen species (ROS) accumulation, decreased mitochondrial membrane potential (MMP), and reduced ATP production. This is evidenced by increased activities of Caspase3 and Caspase9, elevated levels of BAX, and decreased expression of BCL-2. LBP intervention (100 μg/mL) effectively scavenged ROS, restored mitochondrial potential, and enhanced antioxidant capacity, as demonstrated by increased superoxide dismutase (SOD) and glutathione (GSH) levels, alongside decreased malondialdehyde (MDA) levels. Furthermore, LBP suppressed apoptotic pathways and enhanced sperm-zona pellucida binding after BDE-209 exposure. A proposed mechanism summarizing this protective pathway is presented in the Graphical Abstract. As a naturally occurring polysaccharide that protects against oxidative stress in sperm, LBP could represent a promising candidate for dietary interventions in populations exposed to elevated levels of BDE-209. This warrants further investigation in in vivo models.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104311"},"PeriodicalIF":2.9,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145495887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0