Journal of thermal biology最新文献_第9页

Extra food buffers thermoregulatory costs during early development but does not reduce heatwave-induced mortality in nestlings of a cavity-nesting raptor 额外的食物缓冲了早期发育过程中的体温调节成本，但并不能降低穴居猛禽雏鸟因热浪引起的死亡率

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2026-01-01 Epub Date: 2025-12-16 DOI: 10.1016/j.jtherbio.2025.104361

Alejandro Corregidor-Castro , Alessandro Berlusconi , Erica Figus , Anna Kyriakopoulou , Jacopo G. Cecere , Michelangelo Morganti , Diego Rubolini , Andrea Pilastro , Andrea Romano

Global climate change is increasing both average temperatures and the occurrence of extreme weather events, such as heatwaves. These changes pose major challenges for wildlife, especially during vulnerable life stages. In altricial bird species, nest-bound offspring are particularly susceptible to thermal stress due to limited behavioural thermoregulation, where high nest temperatures can impair development and induce elevated mortality, particularly when exceeding critical thresholds. We experimentally manipulated nest temperature and food availability in lesser kestrels (Falco naumanni) rearing their nestlings in nest boxes to investigate whether compensatory feeding offsets the negative effects of high nest temperatures on offspring size and pre-fledging survival. Nestlings were reared under three conditions: control (high nest temperatures), shaded (reduced temperature), and food-supplemented (high temperature with extra food). While parental provisioning rates did not differ among experimental groups, nestlings in food-supplemented boxes showed greater body size than control nestlings, despite similar thermal exposure, and matched the size of nestlings in shaded boxes. This indicates that increased food can buffer the energetic costs of thermoregulation under elevated, but non-lethal, temperatures. Mortality during development did not differ significantly among groups, as temperatures remained below critical thresholds. However, during a naturally occurring heatwave, mortality in control and food-supplemented nests was similarly high (∼40 %), whereas it was very limited in shaded nest boxes (∼4 %). These results suggest that food supplementation cannot prevent heatwave-induced mortality, likely caused by hyperthermia rather than starvation/dehydration. Nest microclimate may thus play a key role in mitigating the negative impacts of intense heatwaves in avian species.

全球气候变化正在增加平均气温和极端天气事件的发生，如热浪。这些变化对野生动物构成了重大挑战，特别是在脆弱的生命阶段。在晚育鸟类中，由于有限的行为体温调节，巢内的后代特别容易受到热应激的影响，巢内的高温会损害发育并导致死亡率升高，特别是在超过临界阈值时。为了研究补偿性喂养是否能抵消高巢温度对雏鸟大小和雏鸟成活率的负面影响，对在巢箱中饲养雏鸟的小红隼（Falco naumanni）进行了调节巢温和食物供应的实验。雏鸟在三种条件下饲养：对照（巢温高）、遮荫（温度降低）和食物补充（高温加额外食物）。虽然各组间的亲代供给率没有差异，但在热暴露相似的情况下，食物补充箱中的雏鸟比对照组雏鸟的体型更大，并且与阴影箱中的雏鸟的体型相匹配。这表明，在升高但非致命的温度下，增加食物可以缓冲体温调节的能量消耗。由于温度保持在临界阈值以下，各组之间发育期间的死亡率没有显著差异。然而，在自然发生的热浪期间，对照巢和食物补充巢的死亡率同样高（~ 40%），而在阴暗的巢箱中，死亡率非常有限（~ 4%）。这些结果表明，食物补充不能防止热浪引起的死亡，可能是由高温而不是饥饿/脱水引起的。因此，巢微气候可能在减轻强烈热浪对鸟类的负面影响方面发挥关键作用。

{"title":"Extra food buffers thermoregulatory costs during early development but does not reduce heatwave-induced mortality in nestlings of a cavity-nesting raptor","authors":"Alejandro Corregidor-Castro , Alessandro Berlusconi , Erica Figus , Anna Kyriakopoulou , Jacopo G. Cecere , Michelangelo Morganti , Diego Rubolini , Andrea Pilastro , Andrea Romano","doi":"10.1016/j.jtherbio.2025.104361","DOIUrl":"10.1016/j.jtherbio.2025.104361","url":null,"abstract":"<div><div>Global climate change is increasing both average temperatures and the occurrence of extreme weather events, such as heatwaves. These changes pose major challenges for wildlife, especially during vulnerable life stages. In altricial bird species, nest-bound offspring are particularly susceptible to thermal stress due to limited behavioural thermoregulation, where high nest temperatures can impair development and induce elevated mortality, particularly when exceeding critical thresholds. We experimentally manipulated nest temperature and food availability in lesser kestrels (<em>Falco naumanni</em>) rearing their nestlings in nest boxes to investigate whether compensatory feeding offsets the negative effects of high nest temperatures on offspring size and pre-fledging survival. Nestlings were reared under three conditions: control (high nest temperatures), shaded (reduced temperature), and food-supplemented (high temperature with extra food). While parental provisioning rates did not differ among experimental groups, nestlings in food-supplemented boxes showed greater body size than control nestlings, despite similar thermal exposure, and matched the size of nestlings in shaded boxes. This indicates that increased food can buffer the energetic costs of thermoregulation under elevated, but non-lethal, temperatures. Mortality during development did not differ significantly among groups, as temperatures remained below critical thresholds. However, during a naturally occurring heatwave, mortality in control and food-supplemented nests was similarly high (∼40 %), whereas it was very limited in shaded nest boxes (∼4 %). These results suggest that food supplementation cannot prevent heatwave-induced mortality, likely caused by hyperthermia rather than starvation/dehydration. Nest microclimate may thus play a key role in mitigating the negative impacts of intense heatwaves in avian species.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"135 ","pages":"Article 104361"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The thermal range of the wild zebra finch in developmental, reproductive, and phylogenetic contexts 野生斑胸草雀在发育、繁殖和系统发育方面的温度范围

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-12-01 Epub Date: 2025-11-20 DOI: 10.1016/j.jtherbio.2025.104340

Simon C. Griffith , Carmen R.B. da Silva , Riccardo Ton

The zebra finch has been used in laboratory studies to understand the effects of ambient temperature on development and reproduction. We characterize the thermal range in which the species breeds in the wild in Australia. Our data reveal that zebra finches breed over an extremely wide range of ambient temperatures (−5.2 °C to 46.2 °C), and that embryos are not as buffered from ambient temperatures as generally considered, regularly spending time at temperatures between 10 °C and 40 °C.

To place the zebra finch in a broader context we examined the thermal breeding range of 327 other Australian terrestrial species. These thermal ranges vary extensively, but there was no indication of a phylogenetic signal for this trait, suggesting it is a relatively labile trait. The range of temperatures in which zebra finch breeds is at the 90th percentile of Australian terrestrial species, indicating that it has a relatively high level of plasticity in coping with thermal conditions. We also found that the zebra finch breeds in relatively high temperatures, with 10 % of observed zebra finch breeding attempts being made in conditions with a 30-day average maximum temperature of 31–38.2 °C. Again, however, around 20 % of Australian bird species were found to breed in higher average maximum temperatures. Nevertheless, the temperatures we have characterized provide insight into the capacity of embryo, nestling, and adult zebra finches to cope across a wide range of ambient temperatures. The zebra finch is a good species for further experimental work in the laboratory to understand tolerance, plasticity, and the effects of temperature on development and physiology. Our findings will help to interpret past and future studies in this important area of research, and provide the appropriate context for future studies to design ecologically relevant manipulations of temperature.

斑马雀已被用于实验室研究，以了解环境温度对其发育和繁殖的影响。我们描述了该物种在澳大利亚野外繁殖的温度范围。我们的数据显示，斑胸草雀在极宽的环境温度范围内繁殖（- 5.2°C至46.2°C），并且胚胎并不像通常认为的那样受到环境温度的缓冲，通常在10°C至40°C之间度过时间。为了将斑胸草雀置于更广阔的背景下，我们研究了327种其他澳大利亚陆生物种的繁殖范围。这些热范围变化很大，但没有迹象表明这一性状有系统发育信号，表明这是一个相对不稳定的性状。斑胸草雀繁殖的温度范围在澳大利亚陆生物种的第90百分位数，表明它在应对温度条件方面具有相对较高的可塑性。我们还发现斑胸草雀在相对较高的温度下繁殖，10%的斑胸草雀在30天平均最高温度为31-38.2°C的条件下繁殖。然而，再次发现大约20%的澳大利亚鸟类在更高的平均最高温度下繁殖。尽管如此，我们所描述的温度为我们了解胚胎、雏鸟和成年斑胸草雀应对各种环境温度的能力提供了依据。斑胸草雀是一个很好的实验物种，可以在实验室中进一步研究耐受性、可塑性以及温度对发育和生理的影响。我们的发现将有助于解释这一重要研究领域过去和未来的研究，并为未来的研究提供适当的背景，以设计与生态相关的温度操纵。

{"title":"The thermal range of the wild zebra finch in developmental, reproductive, and phylogenetic contexts","authors":"Simon C. Griffith , Carmen R.B. da Silva , Riccardo Ton","doi":"10.1016/j.jtherbio.2025.104340","DOIUrl":"10.1016/j.jtherbio.2025.104340","url":null,"abstract":"<div><div>The zebra finch has been used in laboratory studies to understand the effects of ambient temperature on development and reproduction. We characterize the thermal range in which the species breeds in the wild in Australia. Our data reveal that zebra finches breed over an extremely wide range of ambient temperatures (−5.2 °C to 46.2 °C), and that embryos are not as buffered from ambient temperatures as generally considered, regularly spending time at temperatures between 10 °C and 40 °C.</div><div>To place the zebra finch in a broader context we examined the thermal breeding range of 327 other Australian terrestrial species. These thermal ranges vary extensively, but there was no indication of a phylogenetic signal for this trait, suggesting it is a relatively labile trait. The range of temperatures in which zebra finch breeds is at the 90th percentile of Australian terrestrial species, indicating that it has a relatively high level of plasticity in coping with thermal conditions. We also found that the zebra finch breeds in relatively high temperatures, with 10 % of observed zebra finch breeding attempts being made in conditions with a 30-day average maximum temperature of 31–38.2 °C. Again, however, around 20 % of Australian bird species were found to breed in higher average maximum temperatures. Nevertheless, the temperatures we have characterized provide insight into the capacity of embryo, nestling, and adult zebra finches to cope across a wide range of ambient temperatures. The zebra finch is a good species for further experimental work in the laboratory to understand tolerance, plasticity, and the effects of temperature on development and physiology. Our findings will help to interpret past and future studies in this important area of research, and provide the appropriate context for future studies to design ecologically relevant manipulations of temperature.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104340"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145570019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermosensitivity of the Microvasculature: Molecular and physiological mechanisms in skeletal muscle – A narrative review 微血管的热敏性：骨骼肌的分子和生理机制综述

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-12-01 Epub Date: 2025-11-08 DOI: 10.1016/j.jtherbio.2025.104331

Sean P. Langan , Douglas J. Casa , Oh Sung Kwon

Chronic heat stress is a potent intervention to improve vascular health, specifically within the cutaneous and skeletal muscle microcirculation. While the role of cutaneous circulation in heat dissipation is well established, emerging evidence suggests that skeletal muscle vasculature also exhibits thermosensitivity, contributing to heat-induced hyperemia beyond metabolic demand. This narrative review explores the molecular and physiological mechanisms underlying thermal hyperemia, focusing on heat-sensitive ion channels (TRPV1, TRPV4), nitric oxide (NO) signaling, and endothelial adaptations. The role of exercise-induced heat production and heat acclimation in promoting vascular plasticity is also examined, with particular emphasis on vascular remodeling and microcirculatory adaptations. Furthermore, this review highlights the pathological consequences of excessive heat exposure, including heat stroke, endothelial dysfunction, and systemic inflammation, emphasizing the delicate balance between adaptive and maladaptive vascular responses to heat stress. Understanding the interplay between heat stress, vascular regulation, and exercise adaptation provides valuable insights into potential therapeutic applications of heat acclimation for individuals with cardiovascular disease, metabolic disorders, and impaired thermoregulation.

慢性热应激是改善血管健康的有效干预措施，特别是在皮肤和骨骼肌微循环中。虽然皮肤循环在散热中的作用已经确立，但新出现的证据表明，骨骼肌血管系统也表现出热敏性，导致热致充血超出代谢需求。本文探讨了热充血的分子和生理机制，重点关注热敏离子通道（TRPV1, TRPV4），一氧化氮（NO）信号传导和内皮细胞适应。运动诱导的产热和热适应在促进血管可塑性中的作用也被检查，特别强调血管重塑和微循环适应。此外，这篇综述强调了过度热暴露的病理后果，包括中暑、内皮功能障碍和全身性炎症，强调了血管对热应激的适应性和非适应性反应之间的微妙平衡。了解热应激、血管调节和运动适应之间的相互作用，为热适应对心血管疾病、代谢紊乱和体温调节受损个体的潜在治疗应用提供了有价值的见解。

{"title":"Thermosensitivity of the Microvasculature: Molecular and physiological mechanisms in skeletal muscle – A narrative review","authors":"Sean P. Langan , Douglas J. Casa , Oh Sung Kwon","doi":"10.1016/j.jtherbio.2025.104331","DOIUrl":"10.1016/j.jtherbio.2025.104331","url":null,"abstract":"<div><div>Chronic heat stress is a potent intervention to improve vascular health, specifically within the cutaneous and skeletal muscle microcirculation. While the role of cutaneous circulation in heat dissipation is well established, emerging evidence suggests that skeletal muscle vasculature also exhibits thermosensitivity, contributing to heat-induced hyperemia beyond metabolic demand. This narrative review explores the molecular and physiological mechanisms underlying thermal hyperemia, focusing on heat-sensitive ion channels (TRPV1, TRPV4), nitric oxide (NO) signaling, and endothelial adaptations. The role of exercise-induced heat production and heat acclimation in promoting vascular plasticity is also examined, with particular emphasis on vascular remodeling and microcirculatory adaptations. Furthermore, this review highlights the pathological consequences of excessive heat exposure, including heat stroke, endothelial dysfunction, and systemic inflammation, emphasizing the delicate balance between adaptive and maladaptive vascular responses to heat stress. Understanding the interplay between heat stress, vascular regulation, and exercise adaptation provides valuable insights into potential therapeutic applications of heat acclimation for individuals with cardiovascular disease, metabolic disorders, and impaired thermoregulation.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104331"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145518698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of muscle temperature on the muscle blood flow-oxygen consumption relationship during single leg isokinetic exercise 单腿等速运动中肌肉温度对肌肉血流耗氧量关系的影响。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-12-01 Epub Date: 2025-11-13 DOI: 10.1016/j.jtherbio.2025.104318

Dominique D. Gagnon , Alexus McCue , Juha E. Peltonen , David Marsh , David MacLean , Olivier Serresse

Purpose

Muscle blood flow (Q̇) and O₂ delivery to local active muscles during exercise are primarily regulated by oxygen consumption (V̇O). Skeletal muscle tissue heating and cooling also independently influence local oxygen transport and uptake but whether tissue temperature regulates the Q̇/V̇O₂ relationship during exercise is unclear. The purpose of this study was to assess the effects of skeletal muscle temperature on local Q̇, V̇O₂, tissue oxygen saturation (%TSI), and the Q̇/V̇O₂ relationship during single-leg isokinetic exercise at increasing intensities.

Methods

Eleven men exercised for 3-min intervals under localized muscle heating (HT; +Δ3°C), cooling (CO; -Δ6°C), and thermoneutral (TN) conditions at 10 %, 30 %, and 50 % of their maximal voluntary contraction (MVC) during single-leg isokinetic contractions. Blood flow occlusions and near-infrared spectroscopy techniques were used to estimate Q̇, V̇O2, and %TSI before and after each exercise bout.

Results

Q̇ significantly increased with muscle heating, whereas muscle cooling decreased V̇O₂ across MVC intensities. Muscle temperature did not significantly change %TSI during exercise. Moreover, the Q̇/V̇O₂relationship was steeper in CO compared to HT (TN: 0.173 ± 0.101, CO: 0.187 ± 0.0655, and HT:0.107 ± 0.0413) (p = 0.009).

Conclusion

This study demonstrated that muscle temperature regulates the local Q̇/V̇O₂ relationship during submaximal incremental isokinetic single-leg exercise.

目的：运动过程中肌肉血流量(Q)和局部活动肌肉的氧气输送主要受耗氧量(V)的调节。骨骼肌组织的加热和冷却也独立影响局部氧的运输和摄取，但组织温度是否调节运动时的Q /V / O2关系尚不清楚。本研究的目的是评估在增加强度的单腿等速运动中，骨骼肌温度对局部Q值、V值、组织氧饱和度（%TSI）和Q值/V值关系的影响。方法：11名男性在局部肌肉加热（HT; +Δ3°C）、冷却（CO; -Δ6°C）和热中性（TN）条件下，在单腿等速收缩时，分别以其最大自愿收缩（MVC）的10%、30%和50%进行3分钟的间歇运动。使用血流闭塞和近红外光谱技术估计每次运动前后的Q值、V值和%TSI。结果：随着肌肉加热，Q值显著增加，而肌肉冷却降低了MVC强度下的V值。运动期间肌肉温度没有显著改变%TSI。此外，CO组的Q /V / o2关系较HT组更为陡峭（TN: 0.173±0.101,CO: 0.187±0.0655,HT:0.107±0.0413）（p = 0.009）。结论：本研究表明，在亚最大增量等速单腿运动中，肌肉温度调节局部Q /V / O2关系。

{"title":"The effects of muscle temperature on the muscle blood flow-oxygen consumption relationship during single leg isokinetic exercise","authors":"Dominique D. Gagnon , Alexus McCue , Juha E. Peltonen , David Marsh , David MacLean , Olivier Serresse","doi":"10.1016/j.jtherbio.2025.104318","DOIUrl":"10.1016/j.jtherbio.2025.104318","url":null,"abstract":"<div><h3>Purpose</h3><div>Muscle blood flow (Q̇) and O<sub>2</sub> delivery to local active muscles during exercise are primarily regulated by oxygen consumption (V̇O). Skeletal muscle tissue heating and cooling also independently influence local oxygen transport and uptake but whether tissue temperature regulates the Q̇/V̇O<sub>2</sub> relationship during exercise is unclear. The purpose of this study was to assess the effects of skeletal muscle temperature on local Q̇, V̇O<sub>2</sub>, tissue oxygen saturation (%TSI), and the Q̇/V̇O<sub>2</sub> relationship during single-leg isokinetic exercise at increasing intensities.</div></div><div><h3>Methods</h3><div>Eleven men exercised for 3-min intervals under localized muscle heating (HT; +Δ3°C), cooling (CO; -Δ6°C), and thermoneutral (TN) conditions at 10 %, 30 %, and 50 % of their maximal voluntary contraction (MVC) during single-leg isokinetic contractions. Blood flow occlusions and near-infrared spectroscopy techniques were used to estimate Q̇, V̇O2, and %TSI before and after each exercise bout.</div></div><div><h3>Results</h3><div>Q̇ significantly increased with muscle heating, whereas muscle cooling decreased V̇O<sub>2</sub> across MVC intensities. Muscle temperature did not significantly change %TSI during exercise. Moreover, the Q̇/V̇O<sub>2</sub>relationship was steeper in CO compared to HT (TN: 0.173 ± 0.101, CO: 0.187 ± 0.0655, and HT:0.107 ± 0.0413) (p = 0.009).</div></div><div><h3>Conclusion</h3><div>This study demonstrated that muscle temperature regulates the local Q̇/V̇O<sub>2</sub> relationship during submaximal incremental isokinetic single-leg exercise.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104318"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

“Agreement study between esophageal and cloacal thermometry or infrared thermography measurements in Eurasian eagle-owl (Bubo bubo)” 欧亚鹰鸮（Bubobubo）食道和肛管温度测量或红外热成像测量的一致性研究。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-12-01 Epub Date: 2025-11-05 DOI: 10.1016/j.jtherbio.2025.104310

Abril Fernández , Casilda Rodríguez , Virginia Moraleda , Irene López , Laura Suárez , Natalia Pastor , Samanta Waxman , Fernando González

Body temperature is a marker of health or disease in wild birds. In avian species, the most common clinical method for determining body temperature is cloacal thermometry; however, there is little information on how it represents central body temperature in most avian species, including the Eurasian eagle-owl. Also, a less invasive method, such as infrared thermography, may reduce stress during clinical procedures. This study aims to perform a concordance analysis between cloacal thermometry and ocular or pedes thermographic measurements taken from Eurasian eagle owls, comparing them to esophageal thermometry, which is considered a good gold standard for its anatomical arrangement. The study was performed during the clinical procedures carried out on owls at the recovery center hospital. Thermometric measurements (esophageal, cloacal) and thermographic images of the pedes and eyes were documented. The highest correlation was observed when esophageal thermometry values were compared to cloacal thermometry values or eye thermography. The obtained agreement values showed a bias (upper limit of agreement (LoA_u)- lower limit of agreement (LoA_l)) of −0.30(0.90 to −0.76), 0.20(2.77 to −0.90), 0.30(2.77 to −0.86), and 0.30(3.62 to −4.16) ^oC, when esophageal thermometry was compared to cloacal thermometry or thermography values obtained from entire eye, medial canthus of the eye, and pedes, respectively. The cloacal temperature had better correlation and agreement with esophageal thermometry than the thermographically studied sites. The ocular temperature showed a closer agreement with esophageal temperature than with the temperature measured at the pedes. Neither cloacal nor thermographic temperature measurements are concordant with esophageal temperature values (>0.5 °C).

体温是野生鸟类健康或疾病的标志。在鸟类中，测定体温最常用的临床方法是肛管测温；然而，关于它如何代表大多数鸟类（包括欧亚鹰鸮）的中心体温的信息很少。此外，一种侵入性较小的方法，如红外热成像，可以减少临床过程中的压力。本研究旨在对欧亚鹰鸮的阴囊温度测量与眼或足部温度测量进行一致性分析，并将其与食道温度测量进行比较，食道温度测量被认为是其解剖结构的良好金标准。这项研究是在康复中心医院对猫头鹰进行的临床程序中进行的。记录体温测量（食道、泄殖腔）和足部和眼睛的热成像图像。当食道测温值与肛肠测温值或眼热成像值比较时，观察到最高的相关性。当食道测温与全眼、眼内眦和足部测温或热成像值进行比较时，得到的一致性值显示偏差(一致性上限（LoAu)-一致性下限（LoAl））分别为-0.30（0.90至-0.76）、0.20（2.77至-0.90）、0.30（2.77至-0.86）和0.30（3.62至-4.16）℃。与热成像研究部位相比，肛肠温度与食道测温具有更好的相关性和一致性。眼温与食道温度的关系比与足部温度的关系更接近。无论是肛管温度还是热成像温度测量值都与食管温度值（>0.5°C）不一致。

{"title":"“Agreement study between esophageal and cloacal thermometry or infrared thermography measurements in Eurasian eagle-owl (Bubo bubo)”","authors":"Abril Fernández , Casilda Rodríguez , Virginia Moraleda , Irene López , Laura Suárez , Natalia Pastor , Samanta Waxman , Fernando González","doi":"10.1016/j.jtherbio.2025.104310","DOIUrl":"10.1016/j.jtherbio.2025.104310","url":null,"abstract":"<div><div>Body temperature is a marker of health or disease in wild birds. In avian species, the most common clinical method for determining body temperature is cloacal thermometry; however, there is little information on how it represents central body temperature in most avian species, including the Eurasian eagle-owl. Also, a less invasive method, such as infrared thermography, may reduce stress during clinical procedures. This study aims to perform a concordance analysis between cloacal thermometry and ocular or pedes thermographic measurements taken from Eurasian eagle owls, comparing them to esophageal thermometry, which is considered a good gold standard for its anatomical arrangement. The study was performed during the clinical procedures carried out on owls at the recovery center hospital. Thermometric measurements (esophageal, cloacal) and thermographic images of the pedes and eyes were documented. The highest correlation was observed when esophageal thermometry values were compared to cloacal thermometry values or eye thermography. The obtained agreement values showed a bias (upper limit of agreement (LoA<sub>u</sub>)- lower limit of agreement (LoA<sub>l</sub>)) of −0.30(0.90 to −0.76), 0.20(2.77 to −0.90), 0.30(2.77 to −0.86), and 0.30(3.62 to −4.16) <sup>o</sup>C, when esophageal thermometry was compared to cloacal thermometry or thermography values obtained from entire eye, medial canthus of the eye, and pedes, respectively. The cloacal temperature had better correlation and agreement with esophageal thermometry than the thermographically studied sites. The ocular temperature showed a closer agreement with esophageal temperature than with the temperature measured at the pedes. Neither cloacal nor thermographic temperature measurements are concordant with esophageal temperature values (>0.5 °C).</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104310"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145482361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic heat exposure disrupts hepatic homeostasis in bullfrog (Aquarana catesbeiana) during metamorphosis: Integrated histopathological, biochemical and transcriptomic evidence 慢性热暴露破坏牛蛙（Aquarana catesbeiana）在变态过程中的肝脏稳态：综合组织病理学，生化和转录组学证据

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-12-01 Epub Date: 2025-12-04 DOI: 10.1016/j.jtherbio.2025.104347

Yinglin He , Hao Ding , Woxing Li , Shulan Wang , Fang Zeng , Chao Xu , Huirong Yang

Climate change has significantly impacted the survival of aquatic animals, particularly anurans, which undergo a sensitive and vulnerable metamorphic process. We studied the disruptions of chronic heat stress on hepatic homeostasis during metamorphosis of the bullfrog (Aquarana catesbeiana). Bullfrog tadpoles were cultivated under high-temperature (T30, 30 °C) and normal-temperature (T24, 24 °C) conditions for 90 days. The study examined liver histological and biochemical characteristics post-metamorphosis (Gosner stage (Gs) 46) and the transcriptional regulatory mechanisms across three metamorphic stages (Gs26, 38, and 44). Histological analysis of post-metamorphic individuals revealed liver tissue damage in bullfrogs due to chronic heat exposure. Biochemistry indicated that heat stress disrupted the immune and oxidative balance, resulting in elevated total antioxidant capacity and malondialdehyde levels, as well as enhanced activities of catalase, superoxide dismutase and glutathione reductase. Chronic heat exposure significantly influenced hepatic gene expression during metamorphosis. 2,819 DEGs were identified in the T24 group, while 3,918 DEGs were detected in the T30 group. Transcriptomic analysis and quantitative real-time polymerase chain reaction (qRT-PCR) validation demonstrated significant differences in metabolic and immune pathways between the two groups. These findings suggest that chronic heat exposure induces liver tissue damage, induces oxidative stress and inflammation and modulates energy metabolism in bullfrogs. This study lays the groundwork for future research on the heat adaptability of aquatic larvae and potential strategies to mitigate heat stress under global warming.

气候变化严重影响了水生动物的生存，特别是无尾动物，它们经历了一个敏感而脆弱的变质过程。我们研究了牛蛙（Aquarana catesbeiana）变态过程中慢性热应激对肝脏稳态的破坏。牛蛙蝌蚪在高温（T30、30℃）和常温（T24、24℃）条件下培养90 d。该研究检查了变质后（Gosner期（Gs） 46）的肝脏组织学和生化特征，以及三个变质期（Gs26、38和44）的转录调控机制。变质后个体的组织学分析显示牛蛙肝组织损伤是由于慢性热暴露。生物化学表明，热应激破坏了免疫和氧化平衡，导致总抗氧化能力和丙二醛水平升高，过氧化氢酶、超氧化物歧化酶和谷胱甘肽还原酶活性增强。慢性热暴露对变态过程中肝脏基因表达有显著影响。T24组检测到2819个deg， T30组检测到3918个deg。转录组学分析和定量实时聚合酶链反应（qRT-PCR）验证表明，两组之间的代谢和免疫途径存在显著差异。这些研究结果表明，慢性热暴露会导致牛蛙肝组织损伤，引起氧化应激和炎症，并调节能量代谢。本研究为进一步研究全球变暖条件下水生幼体的热适应性和减轻热应激的潜在策略奠定了基础。

{"title":"Chronic heat exposure disrupts hepatic homeostasis in bullfrog (Aquarana catesbeiana) during metamorphosis: Integrated histopathological, biochemical and transcriptomic evidence","authors":"Yinglin He , Hao Ding , Woxing Li , Shulan Wang , Fang Zeng , Chao Xu , Huirong Yang","doi":"10.1016/j.jtherbio.2025.104347","DOIUrl":"10.1016/j.jtherbio.2025.104347","url":null,"abstract":"<div><div>Climate change has significantly impacted the survival of aquatic animals, particularly anurans, which undergo a sensitive and vulnerable metamorphic process. We studied the disruptions of chronic heat stress on hepatic homeostasis during metamorphosis of the bullfrog (<em>Aquarana catesbeiana</em>). Bullfrog tadpoles were cultivated under high-temperature (T30, 30 °C) and normal-temperature (T24, 24 °C) conditions for 90 days. The study examined liver histological and biochemical characteristics post-metamorphosis (Gosner stage (Gs) 46) and the transcriptional regulatory mechanisms across three metamorphic stages (Gs26, 38, and 44). Histological analysis of post-metamorphic individuals revealed liver tissue damage in bullfrogs due to chronic heat exposure. Biochemistry indicated that heat stress disrupted the immune and oxidative balance, resulting in elevated total antioxidant capacity and malondialdehyde levels, as well as enhanced activities of catalase, superoxide dismutase and glutathione reductase. Chronic heat exposure significantly influenced hepatic gene expression during metamorphosis. 2,819 DEGs were identified in the T24 group, while 3,918 DEGs were detected in the T30 group. Transcriptomic analysis and quantitative real-time polymerase chain reaction (qRT-PCR) validation demonstrated significant differences in metabolic and immune pathways between the two groups. These findings suggest that chronic heat exposure induces liver tissue damage, induces oxidative stress and inflammation and modulates energy metabolism in bullfrogs. This study lays the groundwork for future research on the heat adaptability of aquatic larvae and potential strategies to mitigate heat stress under global warming.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104347"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental of thermal field and human activities on particle distribution of home peritoneal dialysis environments in winter 冬季家庭腹膜透析环境中热场与人体活动对颗粒分布的影响

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-12-01 Epub Date: 2025-12-04 DOI: 10.1016/j.jtherbio.2025.104349

Tao Li , Meng Wei , Huapan Shu , Aiping Han , Min Chen , Lamei Liu , Xiaoqing Li

The thermal environment is a critical factor for the safety of home peritoneal dialysis (PD), where sterile procedures are repeatedly performed under winter heating conditions. Yet, little is known about how thermal fields influence airborne particle dynamics in such settings. In this study, a full-scale experimental chamber was constructed to reproduce a typical winter PD room. Representative activities, including cleaning, window opening, air-conditioning operation, and walking, were systematically examined under controlled thermal conditions. Particle concentrations, temperature stratification, and airflow patterns were continuously monitored. Results show that cleaning-induced resuspension delays particle settling, requiring at least 25 min for recovery. medium air supply established a stable thermal field that balanced heating efficiency with particle control, while high speed generated turbulence and increased PM2.5 by more than 40 %. Short-time natural ventilation disrupted the thermal barrier, elevating PM2.5 by 17 % and prolonging coarse particle suspension. Repeated human entries produced cumulative effects, with three entries raising fine particle levels by 47 %. The thermal environment has a significant impact on particle distribution and exposure risk. Stable stratification improves air cleanliness, whereas air-conditioning and window opening disturb stratification and increase aerosol exposure. The observed thermal and aerosol interactions provide experimental evidence and practical strategies to strengthen thermal regulation and reduce infection risk in home peritoneal dialysis.

热环境是家庭腹膜透析（PD）安全的关键因素，在冬季加热条件下反复进行无菌程序。然而，在这种情况下，人们对热场如何影响空气中的粒子动力学知之甚少。在本研究中，构建了一个全尺寸的实验室内，以重现典型的冬季PD室。有代表性的活动，包括清洁、开窗、空调操作和步行，在受控的热条件下被系统地检查。连续监测颗粒浓度、温度分层和气流模式。结果表明，清洗引起的再悬浮延迟了颗粒沉降，至少需要25分钟才能恢复。中等送风建立了稳定的热场，平衡了热效率和颗粒控制，而高速送风产生湍流，使PM2.5增加40%以上。短时间的自然通风破坏了热障，使PM2.5升高了17%，并延长了粗颗粒悬浮时间。人类反复进入会产生累积效应，三次进入会使细颗粒物水平提高47%。热环境对颗粒分布和暴露风险有显著影响。稳定的分层改善空气洁净度，而空调和开窗干扰分层并增加气溶胶暴露。观察到的热与气溶胶相互作用为加强热调节和降低家庭腹膜透析感染风险提供了实验证据和实用策略。

{"title":"Experimental of thermal field and human activities on particle distribution of home peritoneal dialysis environments in winter","authors":"Tao Li , Meng Wei , Huapan Shu , Aiping Han , Min Chen , Lamei Liu , Xiaoqing Li","doi":"10.1016/j.jtherbio.2025.104349","DOIUrl":"10.1016/j.jtherbio.2025.104349","url":null,"abstract":"<div><div>The thermal environment is a critical factor for the safety of home peritoneal dialysis (PD), where sterile procedures are repeatedly performed under winter heating conditions. Yet, little is known about how thermal fields influence airborne particle dynamics in such settings. In this study, a full-scale experimental chamber was constructed to reproduce a typical winter PD room. Representative activities, including cleaning, window opening, air-conditioning operation, and walking, were systematically examined under controlled thermal conditions. Particle concentrations, temperature stratification, and airflow patterns were continuously monitored. Results show that cleaning-induced resuspension delays particle settling, requiring at least 25 min for recovery. medium air supply established a stable thermal field that balanced heating efficiency with particle control, while high speed generated turbulence and increased PM2.5 by more than 40 %. Short-time natural ventilation disrupted the thermal barrier, elevating PM2.5 by 17 % and prolonging coarse particle suspension. Repeated human entries produced cumulative effects, with three entries raising fine particle levels by 47 %. The thermal environment has a significant impact on particle distribution and exposure risk. Stable stratification improves air cleanliness, whereas air-conditioning and window opening disturb stratification and increase aerosol exposure. The observed thermal and aerosol interactions provide experimental evidence and practical strategies to strengthen thermal regulation and reduce infection risk in home peritoneal dialysis.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104349"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145680854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First characterisation of segment-specific teat thermography in dairy goats: rapid temperature dynamics before and after milking 奶山羊节段特异性乳头热成像的第一个特征：挤奶前后的快速温度动态。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-12-01 Epub Date: 2025-11-15 DOI: 10.1016/j.jtherbio.2025.104336

Atakan Çortu, Gökhan Bozkurt

Infrared thermography (IRT) offers a non invasive method to assess skin surface temperature (SST) and detect physiological changes in teat tissue. However, segment specific SST dynamics in dairy goats during milking have not been previously characterised. This study aimed to quantify the magnitude and direction of SST changes in different teat segments before and after milking. Twenty four clinically healthy Saanen goats, aged 2–5 years (mean body weight 63.10 ± 5.65 kg) in mid lactation, were evaluated. Thermal images were captured immediately before milking (T1), and at 30 s (T2), 5 min (T3), and 10 min (T4) after milking. Each teat was divided into base (B), centre (C), and tip (T) segments, and average (Tavg) and maximum (Tmax) SST were extracted from rectangular regions of interest (5 x 25 pixels). Time significantly affected both Tavg and Tmax across all segments (P < 0.001). SST declined between T1/T2 and T3, followed by partial recovery at T4. The teat tip exhibited the largest changes (Tavg d range: 1.76 to +1.84; Tmax d range: 2.11 to +1.11), indicating high mechanical and vascular responsiveness. No left–right asymmetry was detected (P > 0.05), and intra-observer repeatability was excellent (ICC = 0.92–0.97). These findings indicate pronounced cooling followed by rewarming, reflecting transient vascular and mechanical stress at the teat end. IRT effectively detected these rapid, segment-specific SST changes in dairy goats during milking. The established 10-min response profile provides reliable baseline data for welfare assessment and milking equipment evaluation, supporting the use of IRT as a sensitive, non-invasive tool for monitoring udder health and physiological responses in small-ruminant dairy systems.

红外热成像（IRT）提供了一种非侵入性的方法来评估皮肤表面温度（SST）和检测皮肤组织的生理变化。然而，在挤奶期间，奶山羊的特定节段海温动态以前没有被描述过。本研究旨在量化挤奶前后不同乳段海温变化的幅度和方向。选取24只临床健康、年龄2-5岁、平均体重63.10±5.65 kg、泌乳中期的沙宁山羊。分别在挤奶前（T1）、挤奶后30 s （T2）、5 min （T3）和10 min （T4）采集热图像。每个样本被划分为基底(B)、中心(C)和尖端(T)，并从感兴趣的矩形区域（5 x 25像素）提取平均（Tavg）和最大（Tmax）海温。时间对Tavg和Tmax均有显著影响（p0.05），且观察者内重复性极好（ICC = 0.92-0.97）。这些发现表明明显的冷却之后再升温，反映了瞬态血管和机械应力在末端。IRT有效地检测了挤奶期间奶山羊体内这些快速的、特定部位的SST变化。建立的10分钟反应谱为福利评估和挤奶设备评估提供了可靠的基线数据，支持将IRT作为一种灵敏、非侵入性的工具，用于监测小反刍动物乳制品系统的乳房健康和生理反应。

{"title":"First characterisation of segment-specific teat thermography in dairy goats: rapid temperature dynamics before and after milking","authors":"Atakan Çortu, Gökhan Bozkurt","doi":"10.1016/j.jtherbio.2025.104336","DOIUrl":"10.1016/j.jtherbio.2025.104336","url":null,"abstract":"<div><div>Infrared thermography (IRT) offers a non invasive method to assess skin surface temperature (SST) and detect physiological changes in teat tissue. However, segment specific SST dynamics in dairy goats during milking have not been previously characterised. This study aimed to quantify the magnitude and direction of SST changes in different teat segments before and after milking. Twenty four clinically healthy Saanen goats, aged 2–5 years (mean body weight 63.10 ± 5.65 kg) in mid lactation, were evaluated. Thermal images were captured immediately before milking (T1), and at 30 s (T2), 5 min (T3), and 10 min (T4) after milking. Each teat was divided into base (B), centre (C), and tip (T) segments, and average (Tavg) and maximum (Tmax) SST were extracted from rectangular regions of interest (5 x 25 pixels). Time significantly affected both Tavg and Tmax across all segments (P < 0.001). SST declined between T1/T2 and T3, followed by partial recovery at T4. The teat tip exhibited the largest changes (Tavg <em>d</em> range: 1.76 to +1.84; Tmax <em>d</em> range: 2.11 to +1.11), indicating high mechanical and vascular responsiveness. No left–right asymmetry was detected (P > 0.05), and intra-observer repeatability was excellent (ICC = 0.92–0.97). These findings indicate pronounced cooling followed by rewarming, reflecting transient vascular and mechanical stress at the teat end. IRT effectively detected these rapid, segment-specific SST changes in dairy goats during milking. The established 10-min response profile provides reliable baseline data for welfare assessment and milking equipment evaluation, supporting the use of IRT as a sensitive, non-invasive tool for monitoring udder health and physiological responses in small-ruminant dairy systems.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104336"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Puerarin alleviates liver injury induced by heat-stress through regulating oxidative stress and apoptosis in broilers 葛根素通过调节氧化应激和细胞凋亡减轻热应激对肉鸡肝损伤的影响。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-12-01 Epub Date: 2025-11-04 DOI: 10.1016/j.jtherbio.2025.104317

Guangqing Xu , Feiyang Ma , Yan Li , Jiajun Miao , Xueqi Zhang , Yongjie Xiong , Shaojun He

With the intensification of global warming, heat stress (HS) has become a key environmental factor restricting the efficient development of broiler cultivation industry.Puerarin possesses a variety of cytoprotective properties, but the mechanism by which it exerts its potential protective effect against HS-induced liver injury in broiler chickens remains to be elucidated. In this study, the protective mechanisms of puerarin on HS-induced liver injury and metabolic disorders in broilers were investigated. Research has demonstrated that HS can result in diminished growth performance, hepatic oxidative stress, hepatocyte pathological damage and hepatic cell apoptosis in broilers. The results demonstrated that the incorporation of puerarin led to a reduction in the FCR, whilst concomitantly enhancing the production performance of HS broilers. Puerarin supplementation significantly lowered AST and ALT levels while increasing HDL and LDL levels in HS broilers. Meanwhile, The antioxidant analysis demonstrated that the incorporation of puerarin into the liver of broilers could lead to a reduction in MDA levels, while simultaneously enhancing T-AOC and SOD levels. Immunofluorescence and immunohistochemistry results indicated that after puerarin treatment, the levels of Bax and Caspase3 decreased, while the level of Bcl-2 increased. In parallel, gene and protein results indicated that the addition of puerarin could reduce the expression levels of Bak1 and Caspase3 and increase the expression level of Bcl-2 in HS broilers. In summary, the present study demonstrates that puerarin has a protective effect on oxidative stress-induced apoptosis, thereby alleviating hepatocyte injury induced by HS.

随着全球变暖的加剧，热应激（HS）已成为制约肉鸡养殖业高效发展的关键环境因素。葛根素具有多种细胞保护作用，但其对hs诱导的肉鸡肝损伤的保护作用机制尚不清楚。本研究旨在探讨葛根素对hs诱导的肉仔鸡肝损伤和代谢紊乱的保护机制。研究表明，HS可导致肉鸡生长性能下降、肝脏氧化应激、肝细胞病理损伤和肝细胞凋亡。结果表明，葛根素的加入降低了肉鸡的料重比，同时提高了肉鸡的生产性能。添加葛根素可显著降低HS肉鸡AST和ALT水平，提高HDL和LDL水平。同时，抗氧化分析表明，葛根素掺入肉仔鸡肝脏可降低MDA水平，同时提高T-AOC和SOD水平。免疫荧光和免疫组织化学结果显示，葛根素治疗后，Bax和Caspase3水平降低，Bcl-2水平升高。同时，基因和蛋白结果表明，添加葛根素可降低HS肉鸡Bak1和Caspase3的表达水平，提高Bcl-2的表达水平。综上所述，本研究表明葛根素对氧化应激诱导的细胞凋亡具有保护作用，从而减轻HS诱导的肝细胞损伤。

{"title":"Puerarin alleviates liver injury induced by heat-stress through regulating oxidative stress and apoptosis in broilers","authors":"Guangqing Xu , Feiyang Ma , Yan Li , Jiajun Miao , Xueqi Zhang , Yongjie Xiong , Shaojun He","doi":"10.1016/j.jtherbio.2025.104317","DOIUrl":"10.1016/j.jtherbio.2025.104317","url":null,"abstract":"<div><div>With the intensification of global warming, heat stress (HS) has become a key environmental factor restricting the efficient development of broiler cultivation industry.Puerarin possesses a variety of cytoprotective properties, but the mechanism by which it exerts its potential protective effect against HS-induced liver injury in broiler chickens remains to be elucidated. In this study, the protective mechanisms of puerarin on HS-induced liver injury and metabolic disorders in broilers were investigated. Research has demonstrated that HS can result in diminished growth performance, hepatic oxidative stress, hepatocyte pathological damage and hepatic cell apoptosis in broilers. The results demonstrated that the incorporation of puerarin led to a reduction in the FCR, whilst concomitantly enhancing the production performance of HS broilers. Puerarin supplementation significantly lowered AST and ALT levels while increasing HDL and LDL levels in HS broilers. Meanwhile, The antioxidant analysis demonstrated that the incorporation of puerarin into the liver of broilers could lead to a reduction in MDA levels, while simultaneously enhancing T-AOC and SOD levels. Immunofluorescence and immunohistochemistry results indicated that after puerarin treatment, the levels of Bax and Caspase3 decreased, while the level of Bcl-2 increased. In parallel, gene and protein results indicated that the addition of puerarin could reduce the expression levels of Bak1 and Caspase3 and increase the expression level of Bcl-2 in HS broilers. In summary, the present study demonstrates that puerarin has a protective effect on oxidative stress-induced apoptosis, thereby alleviating hepatocyte injury induced by HS.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104317"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145489034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of temperature on early life stages of silver pompano, Trachinotus blochii 温度对银鲳鱼生命早期阶段的影响。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-12-01 Epub Date: 2025-11-12 DOI: 10.1016/j.jtherbio.2025.104328

Krishna Sukumaran , P.S. Pooja , Ambarish P. Gop , Anuraj Anirudhan , G. Rathi Bhuvaneswari , Archana Satheesh , Liyakhath Ali , Muhammed Arshadudeen , S. Nisha , A.P. Dineshbabu , B. Santhosh , V.V.R. Suresh , A. Gopalakrishnan , Grinson George

Silver pompano, Trachinotus blochii is an important mariculture species in India and Southeast Asia. Understanding the influence of temperature, the most critical environmental factor on the early life stages is crucial for identifying the optimal rearing temperature for its hatchery production and assessing the species resilience to climate change. This study evaluated embryogenesis, hatching, yolk-sac larval development and survival at four environmental temperatures, 24 ± 0.5 °C, 27 ± 0.5 °C, 30 ± 0.5 °C and 33 ± 0.5 °C in thermostatically controlled 50-L tanks (in triplicates). It was found that higher temperatures significantly (P < 0.05) accelerated embryogenesis and the shortest developmental time was recorded at 33 °C (14.5 ± 2.6 h). A significantly higher hatching rate (P < 0.05) was recorded at 30 °C, this was accompanied by a significantly (P < 0.05) reduced hatching duration at the higher temperatures. The larvae that hatched at 24 °C had highest total length whereas the total larval length recorded at onset of exogenous feeding was significantly (P < 0.05) higher at 33 °C. The larval activity, utilisation of endogenous energy reserves (yolk sac volume and oil globule) was also significantly (P < 0.05) higher at 30 °C and 33 °C. Although some embryonic and larval development parameters were comparable at 30 °C and 33 °C, there was a significant incidence of morphological abnormalities (10.00 ± 4.08 %), reduction in hatching % and survival of yolk-sac larvae at 33 °C. These results indicate that 30 ± 0.5 °C is the optimal thermal environment for rearing of early stages of silver pompano as it minimises developmental abnormalities, optimises survival and enhances the efficiency of its hatchery production.

银鲳（Trachinotus blochii）是印度和东南亚重要的海水养殖品种。了解温度这一最关键的环境因子对其生命早期阶段的影响，对于确定其孵化场生产的最佳饲养温度和评估物种对气候变化的适应能力至关重要。本研究在恒温控制的50-L培养皿中（3个），分别在24±0.5°C、27±0.5°C、30±0.5°C和33±0.5°C四种环境温度下，评估了胚胎发生、孵化、卵囊幼虫的发育和存活率。结果表明，较高的温度显著（P < 0.05）促进了胚胎的发生，33℃时发育时间最短（14.5±2.6 h）。30°C条件下的孵化率显著高于（P < 0.05），高温条件下的孵化时间显著缩短（P < 0.05）。在24°C时孵化的幼虫总长度最高，而在33°C时孵化的幼虫总长度显著（P < 0.05）高于24°C。在30°C和33°C条件下，幼虫活力、内源能量储备（卵黄囊体积和油球）利用率也显著（P < 0.05）提高。虽然在30°C和33°C下，一些胚胎和幼虫的发育参数相当，但在33°C下，卵黄囊幼虫的形态异常发生率显著（10.00±4.08%），孵化率和存活率降低。以上结果表明，30±0.5°C是早期鲳鱼养殖的最佳温度环境，可以最大限度地减少发育异常，优化成活率，提高孵化场生产效率。

{"title":"Influence of temperature on early life stages of silver pompano, Trachinotus blochii","authors":"Krishna Sukumaran , P.S. Pooja , Ambarish P. Gop , Anuraj Anirudhan , G. Rathi Bhuvaneswari , Archana Satheesh , Liyakhath Ali , Muhammed Arshadudeen , S. Nisha , A.P. Dineshbabu , B. Santhosh , V.V.R. Suresh , A. Gopalakrishnan , Grinson George","doi":"10.1016/j.jtherbio.2025.104328","DOIUrl":"10.1016/j.jtherbio.2025.104328","url":null,"abstract":"<div><div>Silver pompano, <em>Trachinotus blochii</em> is an important mariculture species in India and Southeast Asia. Understanding the influence of temperature, the most critical environmental factor on the early life stages is crucial for identifying the optimal rearing temperature for its hatchery production and assessing the species resilience to climate change. This study evaluated embryogenesis, hatching, yolk-sac larval development and survival at four environmental temperatures, 24 ± 0.5 °C, 27 ± 0.5 °C, 30 ± 0.5 °C and 33 ± 0.5 °C in thermostatically controlled 50-L tanks (in triplicates). It was found that higher temperatures significantly (<em>P <</em> 0.05) accelerated embryogenesis and the shortest developmental time was recorded at 33 °C (14.5 ± 2.6 h). A significantly higher hatching rate (<em>P <</em> 0.05) was recorded at 30 °C, this was accompanied by a significantly (<em>P <</em> 0.05) reduced hatching duration at the higher temperatures. The larvae that hatched at 24 °C had highest total length whereas the total larval length recorded at onset of exogenous feeding was significantly (<em>P <</em> 0.05) higher at 33 °C. The larval activity, utilisation of endogenous energy reserves (yolk sac volume and oil globule) was also significantly (<em>P <</em> 0.05) higher at 30 °C and 33 °C. Although some embryonic and larval development parameters were comparable at 30 °C and 33 °C, there was a significant incidence of morphological abnormalities (10.00 ± 4.08 %), reduction in hatching % and survival of yolk-sac larvae at 33 °C. These results indicate that 30 ± 0.5 °C is the optimal thermal environment for rearing of early stages of silver pompano as it minimises developmental abnormalities, optimises survival and enhances the efficiency of its hatchery production.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104328"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145564410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0