Journal of thermal biology最新文献_第8页

The thermal range of the wild zebra finch in developmental, reproductive, and phylogenetic contexts 野生斑胸草雀在发育、繁殖和系统发育方面的温度范围

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-20 DOI: 10.1016/j.jtherbio.2025.104340

Simon C. Griffith , Carmen R.B. da Silva , Riccardo Ton

The zebra finch has been used in laboratory studies to understand the effects of ambient temperature on development and reproduction. We characterize the thermal range in which the species breeds in the wild in Australia. Our data reveal that zebra finches breed over an extremely wide range of ambient temperatures (−5.2 °C to 46.2 °C), and that embryos are not as buffered from ambient temperatures as generally considered, regularly spending time at temperatures between 10 °C and 40 °C.

To place the zebra finch in a broader context we examined the thermal breeding range of 327 other Australian terrestrial species. These thermal ranges vary extensively, but there was no indication of a phylogenetic signal for this trait, suggesting it is a relatively labile trait. The range of temperatures in which zebra finch breeds is at the 90th percentile of Australian terrestrial species, indicating that it has a relatively high level of plasticity in coping with thermal conditions. We also found that the zebra finch breeds in relatively high temperatures, with 10 % of observed zebra finch breeding attempts being made in conditions with a 30-day average maximum temperature of 31–38.2 °C. Again, however, around 20 % of Australian bird species were found to breed in higher average maximum temperatures. Nevertheless, the temperatures we have characterized provide insight into the capacity of embryo, nestling, and adult zebra finches to cope across a wide range of ambient temperatures. The zebra finch is a good species for further experimental work in the laboratory to understand tolerance, plasticity, and the effects of temperature on development and physiology. Our findings will help to interpret past and future studies in this important area of research, and provide the appropriate context for future studies to design ecologically relevant manipulations of temperature.

斑马雀已被用于实验室研究，以了解环境温度对其发育和繁殖的影响。我们描述了该物种在澳大利亚野外繁殖的温度范围。我们的数据显示，斑胸草雀在极宽的环境温度范围内繁殖（- 5.2°C至46.2°C），并且胚胎并不像通常认为的那样受到环境温度的缓冲，通常在10°C至40°C之间度过时间。为了将斑胸草雀置于更广阔的背景下，我们研究了327种其他澳大利亚陆生物种的繁殖范围。这些热范围变化很大，但没有迹象表明这一性状有系统发育信号，表明这是一个相对不稳定的性状。斑胸草雀繁殖的温度范围在澳大利亚陆生物种的第90百分位数，表明它在应对温度条件方面具有相对较高的可塑性。我们还发现斑胸草雀在相对较高的温度下繁殖，10%的斑胸草雀在30天平均最高温度为31-38.2°C的条件下繁殖。然而，再次发现大约20%的澳大利亚鸟类在更高的平均最高温度下繁殖。尽管如此，我们所描述的温度为我们了解胚胎、雏鸟和成年斑胸草雀应对各种环境温度的能力提供了依据。斑胸草雀是一个很好的实验物种，可以在实验室中进一步研究耐受性、可塑性以及温度对发育和生理的影响。我们的发现将有助于解释这一重要研究领域过去和未来的研究，并为未来的研究提供适当的背景，以设计与生态相关的温度操纵。

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引用次数: 0

Expression dynamics of Acetyl Coenzyme A Carboxylase-Alpha and Cyclooxygenase-2 genes in two indigenous goat breeds of Kerala under heat stress 热胁迫下喀拉拉邦两个地方山羊品种乙酰辅酶A、羧化酶α和环氧合酶2基因的表达动态

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-20 DOI: 10.1016/j.jtherbio.2025.104342

Vrindha Madhavan , Varuna P. Panicker , Jini Roy , M.V. Chinnu , R. Uma

Heat stress and its impacts adversely affect all living organisms, especially domestic animals. Rising body temperature above core temperature has been reported to affect the production, reproduction and disease resistance capacity in animals. The present study was conducted to investigate the impact of heat stress on milk fatty acid synthesis, serum lipid profile, thyroid hormone levels, glucose concentration and oxidative stress parameters in Malabari and Attappady Black goats. Six Malabari and six Attappady Black goats of uniform age and parity were selected for the study. Serum metabolite profiling showed no significant differences between the breeds. The gene expression of ACACA and COX-2 was analysed using quantitative real-time PCR. ACACA exhibited no significant difference (P > 0.05) between the breeds, whereas COX-2 showed a significant difference (P ≤ 0.05). A four-fold increase was observed in COX-2 gene expression in Attappady Black goats. Breed-specific responses to heat stress were observed, with significant changes in oxidative stress parameters and insignificant effects on milk fatty acid synthesis and serum metabolite profile. These findings revealed that Attappady Black goats have higher stress-related gene activation, indicating breed-specific physiological adaptations to heat stress. This may help in selective breeding and management practices for increased resilience.

热应激及其影响对所有生物，特别是家畜产生不利影响。据报道，体温高于核心温度会影响动物的生产、繁殖和抗病能力。本试验旨在研究热应激对马拉巴里和阿塔帕迪黑山羊乳脂肪酸合成、血脂、甲状腺激素水平、葡萄糖浓度和氧化应激参数的影响。选取年龄、胎次均匀的马拉巴里黑山羊和阿塔帕迪黑山羊各6只。血清代谢物分析显示品种间无显著差异。采用实时荧光定量PCR法分析ACACA和COX-2基因的表达。ACACA在品种间无显著差异（P > 0.05）， COX-2在品种间差异显著（P≤0.05）。COX-2基因在阿塔帕迪黑山羊中的表达增加了4倍。不同品种对热应激有不同的反应，氧化应激参数变化显著，对乳脂肪酸合成和血清代谢物谱的影响不显著。这些发现表明，阿塔帕迪黑山羊具有更高的应激相关基因激活，表明品种对热应激的生理适应。这可能有助于提高适应力的选择性育种和管理实践。

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引用次数: 0

Chronic heat stress induces hepatic injury, alters hematological parameters, thermotolerance response in gilts through liver multi-omics analysis 通过肝脏多组学分析，慢性热应激对后备母猪肝脏损伤、血液学参数和热耐受反应的影响。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-18 DOI: 10.1016/j.jtherbio.2025.104319

Jia Luo , Qiang Pu , Zihan Ma , Zhenhao Wen , Jie Chai , Li Chen , Xiaoqian Wu , Zonggang Luo , Tenghao Wang , Jingyong Wang

Global climate warming elevates temperatures, increasing pigs' heat stress susceptibility and metabolic heat production, thereby intensifying heat-related challenges. This study examines chronic heat stress (HS) effects on hematological traits and hepatic responses in gilts. During HS, respiratory rate and surface temperature rose significantly, while rectal temperature stabilized. In the HS group, white blood cell count, aonocyte percentage, eosinophil percentage, absolute eosinophil count, absolute neutrophil count, absolute monocyte count, absolute basophil count, hematocrit, and mean corpuscular hemoglobin concentration decreased markedly, whereas mean platelet volume increased. Blood Beecf and PO₂ levels rose, and PCO₂ levels dropped. HS elevated cortisol, altered thyroid function (higher TSH, lower T3/T4), reduced ACTH indicating adrenal dysfunction, and disrupted reproductive hormones (higher LH, lower FSH) alongside elevated insulin (INS) and estradiol (E2) levels. Furthermore, Albumin/Globulin Ratio (A/G ratio) and Albumin (ALB) decreased, while Alkaline Phosphatase (ALP), Gamma-Glutamyl Transferase (GGT), Globulin (GLB), Total Bile Acids (TBA), and Total Bilirubin (TBIL) increased. Histopathology revealed hepatic punctate necrosis, lymphocyte infiltration, sinusoidal dilation, and hydropic degeneration. Immunofluorescence showed increased Bax⁺ and CD3⁺ cells but decreased Bcl-2⁺ and CD3⁺ cells. Hepatocyte HSP70/90 levels surged. Multi-omics analysis indicated HS enriched oxidative phosphorylation, steroid biosynthesis, lipid metabolism, and thyroid hormone synthesis in the liver. Pro-inflammatory pathways (Calcitriol, LPC 19:0) were activated, while protective molecules (Cholecalciferol, L-Glutathione) countered oxidative stress. As expected, chronic HS impairs liver function, induces an inflammation-antioxidant imbalance. More importantly, HS accelerates the conversion of cholesterol to sex hormones to compensate for ovarian suppression, ultimately affecting gilt reproductive performance.

全球气候变暖使温度升高，增加了猪的热应激易感性和代谢性产热，从而加剧了与热有关的挑战。本研究探讨了慢性热应激（HS）对后备母猪血液学特性和肝脏反应的影响。HS时呼吸频率和体表温度明显升高，直肠温度稳定。HS组白细胞计数、单核细胞百分比、嗜酸性粒细胞百分比、嗜酸性粒细胞绝对计数、中性粒细胞绝对计数、单核细胞绝对计数、嗜碱性粒细胞绝对计数、红细胞压积、红细胞血红蛋白平均浓度显著降低，血小板平均体积升高。血牛肉和PO2水平上升，二氧化碳分压水平下降。HS使皮质醇升高，甲状腺功能改变（TSH升高，T3/T4降低），ACTH降低表明肾上腺功能障碍，生殖激素紊乱（LH升高，FSH降低），同时胰岛素（INS）和雌二醇（E2）水平升高。白蛋白/球蛋白比（A/G Ratio）和白蛋白（ALB）降低，碱性磷酸酶（ALP）、γ -谷氨酰转移酶（GGT）、球蛋白（GLB）、总胆汁酸（TBA）和总胆红素（TBIL）升高。组织病理显示肝点状坏死、淋巴细胞浸润、窦状窦扩张、积水变性。免疫荧光显示Bax+和CD3+细胞增多，Bcl-2+和CD3+细胞减少。肝细胞HSP70/90水平升高。多组学分析表明，HS在肝脏中富集氧化磷酸化、类固醇生物合成、脂质代谢和甲状腺激素合成。促炎途径（骨化三醇，LPC 19:0）被激活，而保护分子（胆骨化醇，l -谷胱甘肽）对抗氧化应激。正如预期的那样，慢性HS损害肝功能，诱导炎症-抗氧化失衡。更重要的是，HS加速了胆固醇向性激素的转化，以补偿卵巢抑制，最终影响后备母猪的生殖性能。

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引用次数: 0

Vertical thermal responses of young adults in campus rooftop spaces during winter in the severe cold regions of China 中国严寒地区冬季校园屋顶青年空间垂直热响应研究

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-17 DOI: 10.1016/j.jtherbio.2025.104316

Bo Wang , Wenlong Zhang , Yutong Guo , Guoqiang Ai , Hongyu Zhao , Wanqi Tao , Bingbing Han

Extreme winter conditions in severe cold regions impose significant physiological strain, challenging human temperature regulation and limiting outdoor space utilisation. This study investigates how vertically stratified thermal environments influence the physiological and perceptual responses of young adults on campus rooftops. Using synchronised microclimate monitoring and thermal perception assessments, we analysed human thermal adaptation across elevation gradients. Key findings are mentioned here: (i) winter thermal regulation is primarily driven by temperature–wind interactions, posing distinct physiological challenges compared to radiation-dominated summer conditions, (ii) the universal thermal climate index demonstrates superior predictive accuracy for thermal sensation across heights compared with physiological equivalent temperature (PET), modified PET and standard effective temperature, especially in east-oriented spaces at high elevations, (iii) thermal neutrality exhibits a non-linear altitudinal pattern, declining from 7.67 °C at 1.5 m to 1.47 °C at 9.5 m before rising to 9.73 °C at 13.5 m, with reduced sensitivity at heights, indicating physiological acclimatisation and (iv) biological and adaptive factors significantly modulate thermal responses, as females and participants from southern origins show broad physiological tolerance limitsand enhanced cold adaptation. These findings enhance our understanding of human thermal adaptation in extreme cold environments and offer physiological evidence for designing thermally optimised vertical spaces in severe cold regions.

严寒地区的极端冬季条件给人体带来了巨大的生理压力，挑战了人体的温度调节，限制了室外空间的利用。本研究探讨了垂直分层的热环境如何影响校园屋顶上年轻人的生理和感知反应。利用同步小气候监测和热感知评估，我们分析了不同海拔梯度的人类热适应。主要发现如下：(i)冬季热调节主要由温度-风相互作用驱动，与辐射主导的夏季条件相比，构成了不同的生理挑战；（ii）与生理等效温度（PET）、修正PET和标准有效温度相比，通用热气候指数对不同高度的热感觉的预测精度更高，特别是在高海拔向东的空间中。（iii）热中性表现出非线性的海拔分布模式，从1.5米高度的7.67°C下降到9.5米高度的1.47°C，然后上升到13.5米高度的9.73°C，在海拔高度的敏感性降低，表明生理适应；（iv）生物和适应因素显著调节热反应，因为雌性和来自南方的参与者表现出广泛的生理耐受极限和增强的冷适应。这些发现增强了我们对人类在极端寒冷环境中的热适应的理解，并为在严寒地区设计热优化的垂直空间提供了生理学证据。

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引用次数: 0

First characterisation of segment-specific teat thermography in dairy goats: rapid temperature dynamics before and after milking 奶山羊节段特异性乳头热成像的第一个特征：挤奶前后的快速温度动态。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-15 DOI: 10.1016/j.jtherbio.2025.104336

Atakan Çortu, Gökhan Bozkurt

Infrared thermography (IRT) offers a non invasive method to assess skin surface temperature (SST) and detect physiological changes in teat tissue. However, segment specific SST dynamics in dairy goats during milking have not been previously characterised. This study aimed to quantify the magnitude and direction of SST changes in different teat segments before and after milking. Twenty four clinically healthy Saanen goats, aged 2–5 years (mean body weight 63.10 ± 5.65 kg) in mid lactation, were evaluated. Thermal images were captured immediately before milking (T1), and at 30 s (T2), 5 min (T3), and 10 min (T4) after milking. Each teat was divided into base (B), centre (C), and tip (T) segments, and average (Tavg) and maximum (Tmax) SST were extracted from rectangular regions of interest (5 x 25 pixels). Time significantly affected both Tavg and Tmax across all segments (P < 0.001). SST declined between T1/T2 and T3, followed by partial recovery at T4. The teat tip exhibited the largest changes (Tavg d range: 1.76 to +1.84; Tmax d range: 2.11 to +1.11), indicating high mechanical and vascular responsiveness. No left–right asymmetry was detected (P > 0.05), and intra-observer repeatability was excellent (ICC = 0.92–0.97). These findings indicate pronounced cooling followed by rewarming, reflecting transient vascular and mechanical stress at the teat end. IRT effectively detected these rapid, segment-specific SST changes in dairy goats during milking. The established 10-min response profile provides reliable baseline data for welfare assessment and milking equipment evaluation, supporting the use of IRT as a sensitive, non-invasive tool for monitoring udder health and physiological responses in small-ruminant dairy systems.

红外热成像（IRT）提供了一种非侵入性的方法来评估皮肤表面温度（SST）和检测皮肤组织的生理变化。然而，在挤奶期间，奶山羊的特定节段海温动态以前没有被描述过。本研究旨在量化挤奶前后不同乳段海温变化的幅度和方向。选取24只临床健康、年龄2-5岁、平均体重63.10±5.65 kg、泌乳中期的沙宁山羊。分别在挤奶前（T1）、挤奶后30 s （T2）、5 min （T3）和10 min （T4）采集热图像。每个样本被划分为基底(B)、中心(C)和尖端(T)，并从感兴趣的矩形区域（5 x 25像素）提取平均（Tavg）和最大（Tmax）海温。时间对Tavg和Tmax均有显著影响（p0.05），且观察者内重复性极好（ICC = 0.92-0.97）。这些发现表明明显的冷却之后再升温，反映了瞬态血管和机械应力在末端。IRT有效地检测了挤奶期间奶山羊体内这些快速的、特定部位的SST变化。建立的10分钟反应谱为福利评估和挤奶设备评估提供了可靠的基线数据，支持将IRT作为一种灵敏、非侵入性的工具，用于监测小反刍动物乳制品系统的乳房健康和生理反应。

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引用次数: 0

The effect of different levels of heat stress on the behaviour of cows at different stage of lactation 不同热应激水平对奶牛泌乳不同阶段行为的影响

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-14 DOI: 10.1016/j.jtherbio.2025.104334

Wissem Baccouri , George Wanjala , Violetta Tóth , István Komlósi , Edit Mikó

Rising temperatures and humidity increasingly challenge the welfare and productivity of dairy cows worldwide. Behavioural changes are among the earliest responses to heat stress, reflecting animals’ efforts to reduce heat load. This study investigated how heat stress and lactation stage interact to influence the behaviour of Holstein Friesian cows. Data were collected from 60 cows between 20 June and July 20, 2024, with 1680 records of eating, ruminating, and inactivity times. Cows were grouped by lactation stage: early (days in milk, (DIM) ≤ 60), mid (DIM 61–180), and late (DIM >180). Behaviour was assessed under moderate (temperature–humidity index, (THI) 72–76) and severe heat stress (THI ≥77). A significant interaction was observed between the level of heat stress and lactation stage. Under severe heat stress, eating time declined significantly across all lactation groups, with the largest reduction observed in late-lactation cows. Ruminating time decreased significantly only in late-lactation cows, while remaining relatively stable in early and mid-lactation. Conversely, inactivity time increased in all groups, with the duration of inactivity rising as lactation advanced. These results suggest that behavioural responses to heat stress intensify with advancing lactation. In conclusion, late-lactation cows are more susceptible to heat stress than those in earlier stages, indicating a need for targeted mitigation strategies to support cow welfare and productivity.

不断上升的温度和湿度日益挑战着全世界奶牛的福利和生产力。行为变化是对热应激最早的反应之一，反映了动物减少热负荷的努力。本研究探讨了热应激和泌乳期相互作用对荷斯坦奶牛行为的影响。研究人员在2024年6月20日至7月20日期间收集了60头奶牛的数据，记录了1680次进食、反刍和不活动的时间。奶牛按泌乳阶段分组：早期（泌乳天数，DIM≤60）、中期（DIM 61 ~ 180）和晚期（DIM >180）。在中度（温度-湿度指数，（THI） 72-76）和重度热应激（THI≥77）下评估行为。热应激水平与哺乳期之间存在显著的相互作用。在严重热应激下，所有泌乳组的进食时间都显著减少，泌乳后期奶牛的减少幅度最大。反刍时间仅在泌乳后期显著减少，在泌乳早期和中期保持相对稳定。相反，所有组的不活动时间都增加了，随着哺乳的进展，不活动的持续时间也增加了。这些结果表明，对热应激的行为反应随着泌乳的提前而增强。综上所述，泌乳后期的奶牛比泌乳早期的奶牛更容易受到热应激的影响，这表明需要有针对性的缓解策略来支持奶牛的福利和生产力。

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引用次数: 0

Temperature-induced ontogenic plasticity and larval fitness of golden mahseer (Tor putitora) in captivity 人工饲养金马尾鱼温度诱导的个体可塑性和幼虫适合度。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-14 DOI: 10.1016/j.jtherbio.2025.104337

Pandey Harsh , Tasaduq Hussain Shah , Alexander Ciji , Pramod Kumar Pandey , Arya Mukul , Md Shahbaz Akhtar

The effects of varying temperature regimes on the ontogenic and early larval development of golden mahseer in captive conditions were investigated. For this, after fertilization, eggs of golden mahseer from a single pair of broodstock were randomly distributed into four incubation temperature regimes (20 °C, 23 °C, 26 °C, and 29 °C) in triplicates, and key parameters like fertilization rates, ontogenic events, hatching windows, yolk-sac absorption, larval growth, survival and fitness were assessed. The embryos of T. putitora developed most rapidly at 29 °C, followed by 26 °C, 23 °C, and 20 °C. No notable differences were observed in the time taken to progress from the 2-cell stage to the late morula stage across temperatures. However, incubation temperatures significantly influenced later stages of development, such as blastodisc formation, notochord onset, twitching, segmentation, optic formation, and hatching. At 29 °C, blastodisc formation, twitching, notochord onset, somite formation, and hatching occurred approximately 1, 9, 6, 11 and 29 h earlier, respectively, compared to 20 °C. Despite the faster development at 29 °C, there was a marked reduction in fertilization, hatching success, and survival. The incubation period was the longest at lower temperatures and decreased significantly as the temperature rose, with a corresponding reduction in the hatching window. Complete yolk-sac absorption was the slowest at 20 °C and faster at higher temperatures. The critical thermal maximum (CTmax) and minimum (CTmin) of larvae increased with incubation and rearing temperatures, peaking at 26 °C. Rearing temperatures also affected the anti-oxidative enzyme activities in the larvae. Overall, these findings highlight the vulnerability of golden mahseer to elevated temperatures and provide insights into how future warming scenarios could further threaten this endangered species.

在圈养条件下，研究了不同温度对马尾蛇个体发育和早期幼虫发育的影响。为此，在受精后，将单对金马尾鱼卵随机分为4个孵育温度区（20°C、23°C、26°C和29°C） 3个重复，评估受精率、个体事件、孵化窗口、卵囊吸收、幼虫生长、存活和适合度等关键参数。29°C时，绒背龙胚胎发育最快，其次是26°C、23°C和20°C。在不同温度下，从2细胞阶段到桑葚晚期所需的时间没有显著差异。然而，孵育温度显著影响后期发育，如囊胚盘形成、脊索发生、抽搐、分割、视神经形成和孵化。与20℃相比，29℃时囊胚盘形成、抽搐、脊索发生、体体形成和孵化分别提前了大约1、9、6、11和29小时。尽管在29°C下发育较快，但受精率、孵化成功率和存活率明显降低。孵育期在温度较低时最长，随着温度的升高孵育期明显缩短，孵育期也相应缩短。卵黄囊完全吸收在20℃时最慢，在更高温度下更快。幼虫的临界热最大值（CTmax）和最小值（CTmin）随孵育温度和饲养温度的升高而升高，在26℃时达到峰值。饲养温度对幼虫抗氧化酶活性也有影响。总的来说，这些发现突出了金马鹿对气温升高的脆弱性，并为未来的变暖情景如何进一步威胁这种濒危物种提供了见解。

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引用次数: 0

Cold-water recovery between bouts of simulated rugby sevens matches in the heat 在炎热的七人制橄榄球模拟比赛之间进行冷水恢复。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-14 DOI: 10.1016/j.jtherbio.2025.104333

Melissa Skein , Jayden Hunter , Alexander Carney

The aim of this study was to investigate the effect of cold-water immersion (CWI) between consecutive bouts of simulated rugby seven's matches on performance, physiology and perception. Using a randomised crossover counterbalanced design, 10 male recreational team-sport athletes completed two trials involving two 14 min simulated self-paced intermittent-sprint protocol (ISP) bouts with a 45 min recovery between each bout including a 15 min recovery intervention. Participants completed resting measures, 15 m sprints and vertical jumps (VJ) followed by the first ISP (ISP1) in a hot environment. After ISP1 participants were either seated in an ice-bath (CWI; 11.6 ± 1.8 °C) (CWI) or sat in a thermo-neutral environment (CONT) for 15 min. Measures of heart rate (HR), core temperature (T_core), skin temperature (T_skin), thermal sensation (TS) and ratings of perceived exertion (RPE) were recorded during ISP1, ISP2 and the recovery period. Statistical significance was set at P = 0.05. Vertical jump height was lower and 15 m sprint times slower during ISP2, following CWI (P = 0.01–0.02). While jogging self-paced efforts from ISP1 to ISP2 were maintained, sprint times were slower, and bounding distance was increased in CWI compared to CONT. Tskin was reduced during recovery for CWI (P = 0.001). Tcore, RPE, and TS were lower throughout ISP2 for CWI (P = 0.001–0.04). Therefore, CWI as a recovery modality between successive bouts of simulated rugby sevens in the heat may negatively affect initial high intensity performance compared to passive recovery yet may attenuate thermoregulatory stress through a pre-cooling effect still present 30 min after CWI, leading to higher intensity during the jogging efforts.

本研究旨在探讨在连续几场模拟橄榄球七人赛之间进行冷水浸泡（CWI）对运动表现、生理和知觉的影响。采用随机交叉平衡设计，10名男性休闲团队运动运动员完成了两项试验，包括两次14分钟模拟自定节奏间歇冲刺协议（ISP）回合，每次回合之间45分钟恢复，包括15分钟恢复干预。参与者在炎热的环境中完成了休息测量，15米短跑和垂直跳跃（VJ），然后是第一次ISP （ISP1）。ISP1后，参与者在冰浴（CWI; 11.6±1.8°C）（CWI）或热中性环境（CONT）中坐下15分钟。记录各组在ISP1、ISP2及恢复期的心率（HR）、核心温度（Tcore）、皮肤温度（Tskin）、热感觉（TS）及感觉运动评分（RPE）。P = 0.05为差异有统计学意义。CWI后，ISP2阶段垂直起跳高度较低，15 m冲刺次数较慢（P = 0.01 ~ 0.02）。当保持从ISP1到ISP2的自定节奏慢跑时，与对照组相比，CWI的冲刺时间更慢，跳跃距离增加，CWI恢复期间的Tskin减少（P = 0.001）。CWI患者的Tcore、RPE和TS在整个ISP2期均较低（P = 0.001-0.04）。因此，与被动恢复相比，CWI作为连续几场模拟七人橄榄球比赛之间的恢复方式，可能会对初始高强度表现产生负面影响，但CWI后30分钟仍存在的预冷效应可能会减弱热调节应激，从而导致慢跑期间的强度更高。

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引用次数: 0

The effects of muscle temperature on the muscle blood flow-oxygen consumption relationship during single leg isokinetic exercise 单腿等速运动中肌肉温度对肌肉血流耗氧量关系的影响。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-13 DOI: 10.1016/j.jtherbio.2025.104318

Dominique D. Gagnon , Alexus McCue , Juha E. Peltonen , David Marsh , David MacLean , Olivier Serresse

Purpose

Muscle blood flow (Q̇) and O₂ delivery to local active muscles during exercise are primarily regulated by oxygen consumption (V̇O). Skeletal muscle tissue heating and cooling also independently influence local oxygen transport and uptake but whether tissue temperature regulates the Q̇/V̇O₂ relationship during exercise is unclear. The purpose of this study was to assess the effects of skeletal muscle temperature on local Q̇, V̇O₂, tissue oxygen saturation (%TSI), and the Q̇/V̇O₂ relationship during single-leg isokinetic exercise at increasing intensities.

Methods

Eleven men exercised for 3-min intervals under localized muscle heating (HT; +Δ3°C), cooling (CO; -Δ6°C), and thermoneutral (TN) conditions at 10 %, 30 %, and 50 % of their maximal voluntary contraction (MVC) during single-leg isokinetic contractions. Blood flow occlusions and near-infrared spectroscopy techniques were used to estimate Q̇, V̇O2, and %TSI before and after each exercise bout.

Results

Q̇ significantly increased with muscle heating, whereas muscle cooling decreased V̇O₂ across MVC intensities. Muscle temperature did not significantly change %TSI during exercise. Moreover, the Q̇/V̇O₂relationship was steeper in CO compared to HT (TN: 0.173 ± 0.101, CO: 0.187 ± 0.0655, and HT:0.107 ± 0.0413) (p = 0.009).

Conclusion

This study demonstrated that muscle temperature regulates the local Q̇/V̇O₂ relationship during submaximal incremental isokinetic single-leg exercise.

目的：运动过程中肌肉血流量(Q)和局部活动肌肉的氧气输送主要受耗氧量(V)的调节。骨骼肌组织的加热和冷却也独立影响局部氧的运输和摄取，但组织温度是否调节运动时的Q /V / O2关系尚不清楚。本研究的目的是评估在增加强度的单腿等速运动中，骨骼肌温度对局部Q值、V值、组织氧饱和度（%TSI）和Q值/V值关系的影响。方法：11名男性在局部肌肉加热（HT; +Δ3°C）、冷却（CO; -Δ6°C）和热中性（TN）条件下，在单腿等速收缩时，分别以其最大自愿收缩（MVC）的10%、30%和50%进行3分钟的间歇运动。使用血流闭塞和近红外光谱技术估计每次运动前后的Q值、V值和%TSI。结果：随着肌肉加热，Q值显著增加，而肌肉冷却降低了MVC强度下的V值。运动期间肌肉温度没有显著改变%TSI。此外，CO组的Q /V / o2关系较HT组更为陡峭（TN: 0.173±0.101,CO: 0.187±0.0655,HT:0.107±0.0413）（p = 0.009）。结论：本研究表明，在亚最大增量等速单腿运动中，肌肉温度调节局部Q /V / O2关系。

{"title":"The effects of muscle temperature on the muscle blood flow-oxygen consumption relationship during single leg isokinetic exercise","authors":"Dominique D. Gagnon , Alexus McCue , Juha E. Peltonen , David Marsh , David MacLean , Olivier Serresse","doi":"10.1016/j.jtherbio.2025.104318","DOIUrl":"10.1016/j.jtherbio.2025.104318","url":null,"abstract":"<div><h3>Purpose</h3><div>Muscle blood flow (Q̇) and O<sub>2</sub> delivery to local active muscles during exercise are primarily regulated by oxygen consumption (V̇O). Skeletal muscle tissue heating and cooling also independently influence local oxygen transport and uptake but whether tissue temperature regulates the Q̇/V̇O<sub>2</sub> relationship during exercise is unclear. The purpose of this study was to assess the effects of skeletal muscle temperature on local Q̇, V̇O<sub>2</sub>, tissue oxygen saturation (%TSI), and the Q̇/V̇O<sub>2</sub> relationship during single-leg isokinetic exercise at increasing intensities.</div></div><div><h3>Methods</h3><div>Eleven men exercised for 3-min intervals under localized muscle heating (HT; +Δ3°C), cooling (CO; -Δ6°C), and thermoneutral (TN) conditions at 10 %, 30 %, and 50 % of their maximal voluntary contraction (MVC) during single-leg isokinetic contractions. Blood flow occlusions and near-infrared spectroscopy techniques were used to estimate Q̇, V̇O2, and %TSI before and after each exercise bout.</div></div><div><h3>Results</h3><div>Q̇ significantly increased with muscle heating, whereas muscle cooling decreased V̇O<sub>2</sub> across MVC intensities. Muscle temperature did not significantly change %TSI during exercise. Moreover, the Q̇/V̇O<sub>2</sub>relationship was steeper in CO compared to HT (TN: 0.173 ± 0.101, CO: 0.187 ± 0.0655, and HT:0.107 ± 0.0413) (p = 0.009).</div></div><div><h3>Conclusion</h3><div>This study demonstrated that muscle temperature regulates the local Q̇/V̇O<sub>2</sub> relationship during submaximal incremental isokinetic single-leg exercise.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"134 ","pages":"Article 104318"},"PeriodicalIF":2.9,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of temperature on early life stages of silver pompano, Trachinotus blochii 温度对银鲳鱼生命早期阶段的影响。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2025-11-12 DOI: 10.1016/j.jtherbio.2025.104328

Krishna Sukumaran , P.S. Pooja , Ambarish P. Gop , Anuraj Anirudhan , G. Rathi Bhuvaneswari , Archana Satheesh , Liyakhath Ali , Muhammed Arshadudeen , S. Nisha , A.P. Dineshbabu , B. Santhosh , V.V.R. Suresh , A. Gopalakrishnan , Grinson George

Silver pompano, Trachinotus blochii is an important mariculture species in India and Southeast Asia. Understanding the influence of temperature, the most critical environmental factor on the early life stages is crucial for identifying the optimal rearing temperature for its hatchery production and assessing the species resilience to climate change. This study evaluated embryogenesis, hatching, yolk-sac larval development and survival at four environmental temperatures, 24 ± 0.5 °C, 27 ± 0.5 °C, 30 ± 0.5 °C and 33 ± 0.5 °C in thermostatically controlled 50-L tanks (in triplicates). It was found that higher temperatures significantly (P < 0.05) accelerated embryogenesis and the shortest developmental time was recorded at 33 °C (14.5 ± 2.6 h). A significantly higher hatching rate (P < 0.05) was recorded at 30 °C, this was accompanied by a significantly (P < 0.05) reduced hatching duration at the higher temperatures. The larvae that hatched at 24 °C had highest total length whereas the total larval length recorded at onset of exogenous feeding was significantly (P < 0.05) higher at 33 °C. The larval activity, utilisation of endogenous energy reserves (yolk sac volume and oil globule) was also significantly (P < 0.05) higher at 30 °C and 33 °C. Although some embryonic and larval development parameters were comparable at 30 °C and 33 °C, there was a significant incidence of morphological abnormalities (10.00 ± 4.08 %), reduction in hatching % and survival of yolk-sac larvae at 33 °C. These results indicate that 30 ± 0.5 °C is the optimal thermal environment for rearing of early stages of silver pompano as it minimises developmental abnormalities, optimises survival and enhances the efficiency of its hatchery production.

银鲳（Trachinotus blochii）是印度和东南亚重要的海水养殖品种。了解温度这一最关键的环境因子对其生命早期阶段的影响，对于确定其孵化场生产的最佳饲养温度和评估物种对气候变化的适应能力至关重要。本研究在恒温控制的50-L培养皿中（3个），分别在24±0.5°C、27±0.5°C、30±0.5°C和33±0.5°C四种环境温度下，评估了胚胎发生、孵化、卵囊幼虫的发育和存活率。结果表明，较高的温度显著（P < 0.05）促进了胚胎的发生，33℃时发育时间最短（14.5±2.6 h）。30°C条件下的孵化率显著高于（P < 0.05），高温条件下的孵化时间显著缩短（P < 0.05）。在24°C时孵化的幼虫总长度最高，而在33°C时孵化的幼虫总长度显著（P < 0.05）高于24°C。在30°C和33°C条件下，幼虫活力、内源能量储备（卵黄囊体积和油球）利用率也显著（P < 0.05）提高。虽然在30°C和33°C下，一些胚胎和幼虫的发育参数相当，但在33°C下，卵黄囊幼虫的形态异常发生率显著（10.00±4.08%），孵化率和存活率降低。以上结果表明，30±0.5°C是早期鲳鱼养殖的最佳温度环境，可以最大限度地减少发育异常，优化成活率，提高孵化场生产效率。

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引用次数: 0