Journal of thermal biology最新文献

{"title":"In the heat of the night: Temperature and vegetation structure disparity in habitat suitability for scaled quail","authors":"Katherine A. Travis ,&nbsp;Caleb M. McKinney ,&nbsp;Evan P. Tanner ,&nbsp;Ashley M. Tanner ,&nbsp;Fidel Hernández ,&nbsp;Leonard A. Brennan ,&nbsp;David G. Hewitt ,&nbsp;Humberto L. Perotto-Baldivieso ,&nbsp;Ryan S. Luna ,&nbsp;R. Dwayne Elmore ,&nbsp;John McLaughlin","doi":"10.1016/j.jtherbio.2026.104381","DOIUrl":"10.1016/j.jtherbio.2026.104381","url":null,"abstract":"<div><div>Animal space use is structured by a combination of biotic and abiotic factors, often resulting in disparity of useable space across gradients of these conditions. Animals experience two fundamentally different environments across the diel cycle, as diurnal and nocturnal periods contain different conditions around which animals must partition their space use. This partitioning is partially a consequence of animals needing to meet different demands across the diel cycle. Abiotic conditions can interact with biotic pressures to cause animals to respond differently to compounding pressures such as thermal stress between the diurnal and nocturnal periods. The chestnut-bellied scaled quail (<em>Callipepla squamata</em> var. <em>castanogastris</em>) is a shrubland-obligate subspecies of scaled quail within the Tamaulipan thornscrub biotic province that has faced population declines in recent decades. Our objectives were to examine the space use of GPS-tagged chestnut-bellied scaled quail at two sites in South Texas (one stable and one declining population) and to predict how habitat suitability shifts across the diel cycle. Additionally, we determined how changes in habitat suitability may be impacted by ambient temperature as well as landscape configuration. While diurnal and nocturnal environmental suitability overlap was relatively high at most temperatures (Schoener's <em>D</em>: 0.67–0.81 [stable site]; 0.70–0.87 [declining site]), we found that overlap decreased by 15 % (stable population site) and 22 % (declining population site) when ambient temperatures reached the upper limit of the species' thermoneutral zone (35 °C). This decrease in environmental suitability overlap occurred at relatively cooler temperatures at the declining population site (32.2°C–35.0 °C) than at the stable population site (35.0°C–37.8 °C). Our results indicate that relatively small changes in temperature can affect habitat suitability across the diel cycle. Understanding disparity in diurnal and nocturnal space use, and the possible consequences of this divergence, is increasingly important in a warming and more anthropogenically altered environment.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104381"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146052991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of hot-dry and hot-humid seasons on the plasma cytokines, hormones, and body surface temperatures in lactating indigenous (Sahiwal) and crossbred (Karan Fries) cows raised in tropical climates","authors":"Manisha Choudhary ,&nbsp;Aarti Kamboj ,&nbsp;Pritam Pal ,&nbsp;Bagavathi M ,&nbsp;Animesh Patel ,&nbsp;Pankaj Suman ,&nbsp;Ajay Kumar Dang","doi":"10.1016/j.jtherbio.2026.104405","DOIUrl":"10.1016/j.jtherbio.2026.104405","url":null,"abstract":"<div><div>Heat stress (HS) significantly challenges dairy cattle, affecting their immune function, endocrine responses, and thermoregulation. This study investigated cytokine responses, hormonal changes, and body surface temperature variations in 12 indigenous Sahiwal (SW) and 12 crossbred Karan Fries (KF) cows reared under hot-dry (HD; THI = 77.75) and hot-humid (HH; THI = 81.48) seasons. Plasma concentrations of Interleukin-6 (IL-10), tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-1β (IL- 1β), cortisol, triiodothyronine (T3), and thyroxine (T4) were analysed using ELISA, while body surface temperatures were measured using infrared thermography (IRT). Significant (P &lt; 0.05) variations were observed between breeds and across the two seasons. Higher TNF-α, IL-6, IL-1β, and cortisol levels were observed under HH compared to HD seasons. TNF-α levels increased significantly (P &lt; 0.05) by 31.76% in SW and 36.47% in KF cows, while cortisol levels rose by 44.41% and 47.42%, respectively, from the HD to HH season. In contrast, T3 and T4 levels declined significantly (P &lt; 0.05) by 25.60% and 36.42%, respectively, under HH seasons, with a greater reduction observed in KF cows. Body surface temperatures were significantly (P &lt; 0.05) higher in KF cows across all measured regions, with eye temperature exhibiting a strong correlation with TNF-α (r = 0.88, P &lt; 0.01) and cortisol (r = 0.78, P &lt; 0.01). These findings highlighted the heightened susceptibility of crossbred cows to HS and also provided important new insights into some stress indicators in native and crossbred cows, which could be integrated into farming systems under hot conditions.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104405"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of cold temperature exposure on markers of bone health in humans: A scoping review","authors":"R. Allan ,&nbsp;H.T. Hurst ,&nbsp;B. Akin ,&nbsp;N. Liles ,&nbsp;J. Dickson ,&nbsp;J. Knill-Jones ,&nbsp;J. Sinclair ,&nbsp;S. Dillon ,&nbsp;S.J. Hesketh ,&nbsp;C. Mawhinney","doi":"10.1016/j.jtherbio.2026.104401","DOIUrl":"10.1016/j.jtherbio.2026.104401","url":null,"abstract":"<div><h3>Background</h3><div>Studies of cold-dwelling humans show lower bone mineral density (BMD) and greater age-related bone loss. While cold exposure is regularly used for analgesic purposes, prolonged use may unintentionally exacerbate symptoms it aims to treat.</div></div><div><h3>Objective</h3><div>To evaluate human evidence on the effects of cold exposure upon markers of bone health.</div></div><div><h3>Methods</h3><div>This scoping review followed the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines. OVID (MEDLINE, EMBASE), Elsevier (ScienceDirect, Scopus), and PubMED databases were searched using the terms (“Cold temperature” OR “cryotherapy” OR “Hydrotherapy” OR “Cold stress”) AND (“Bone Matrix” OR “Bone mineral density” OR “bone formation” OR “bone remodelling” OR Osteogenesis). Inclusion criteria were: (1) cold exposure or application in humans; (2) measurement of bone health or turnover markers; and (3) original research or case reports.</div></div><div><h3>Results</h3><div>Searches resulted in 2372 articles, yielding a final pool of 13 articles for inclusion after screening. Several cooling methods were used, predominantly cold air (n = 6), cooling vests (n = 4), sleeping in cold rooms (n = 1), β3 agonist coupled with cooling pads (n = 1) and ice water swimming (n = 3). Markers of bone health were assessed, with BMD shown to increase following cryotherapy, and having a positive correlation with brown adipose tissue. Papers returned suggest a neutral or positive response in factors that enhance osteoblast differentiation (irisin, insulin-like growth factor-1 [IGF-1], meteorin-like protein [METRNL]), which fails to translate directly into improved bone formation (procollagen 1 N-terminal propeptide [P1NP], osteocalcin).</div></div><div><h3>Conclusion</h3><div>Cold exposure might dampen bone resorption mechanisms (beta isomerised C terminal telopeptide of type 1 collagen [β-CTX]) but not others (receptor activator of nuclear factor <em>κ</em> B [RANK], RANK ligand [RANKL], interleukin-6 [IL-6]). Hormonal markers of bone remodelling (thyroid stimulating hormone [TSH], parathyroid hormone [PTH], triiodothyronine [T3]) also showed contrasting effects. As such, the limited data does not allow for clear conclusions.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104401"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hard to measure Lilliputians: Error in the measurement of body temperature of small amphibians and a method to avoid it","authors":"Georgia K. Kosmala,&nbsp;Enore A. Massoni,&nbsp;Denis V. Andrade","doi":"10.1016/j.jtherbio.2026.104391","DOIUrl":"10.1016/j.jtherbio.2026.104391","url":null,"abstract":"<div><div>Accurate measurement of body temperature (T_b) is essential for understanding the physiological ecology of ectothermic organisms. However, in small-bodied amphibians, heat exchange during handling and intrinsic limitations of measuring devices and/or protocols can introduce substantial error in T_b measurements. Here, we first estimated the magnitude of such an error for methodological approaches commonly adopted in the measurement of T_b in small ectothermic organisms in the field. We then propose a novel, low-cost restraint method, using a modified Foerster forceps, aimed at reducing heat transfer during handling. Accordingly, we compared the short-term changes (&lt;2 min) in the T_b of frogs restrained with bare or gloved hands with those measured under the new proposed restraint method. We performed all tests using the Hylidae treefrog <em>Dendropsophus nanus</em>, whose small size made it perfectly suited to our goals, and we also repeated the experiments at three ambient temperatures (15 °C, 25 °C, and 35 °C), which allowed us to evaluate the influence of this variable on the measurement error. Our results clearly demonstrate that heat transfer from the researcher's hand to a small-bodied frog causes large deviations in the measured T_b and that those changes happen at an amazingly fast pace (within seconds) and are most prominent at lower ambient temperatures. The largely preconized use of gloves during measurements indeed reduced the error in T_b measurements to a moderate extent; however, the novel Foerster method herein proposed resulted in minimal change in frog's T_b and proved to be a superior and viable alternative to improve the accuracy of T_b measurement in small-bodied ectotherms.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104391"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146046840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat hardening enhances mosquito heat tolerance in a species-specific and assay-specific manner","authors":"Apeksha L. Warusawithana,&nbsp;Belinda van Heerwaarden,&nbsp;Ary A. Hoffmann,&nbsp;Perran A. Ross","doi":"10.1016/j.jtherbio.2026.104394","DOIUrl":"10.1016/j.jtherbio.2026.104394","url":null,"abstract":"<div><div>Models predict that the distribution of many mosquito species will shift with climate change, but few incorporate adaptive capacity. Acclimation is one mechanism that allows mosquitoes previously exposed to sub-lethal stress to better tolerate subsequent stress. In this study we evaluated the heat tolerance of three vector mosquitoes, <em>Aedes aegypti</em>, <em>Ae. notoscriptus</em> and <em>Culex quinquefasciatus,</em> after prior exposure to heat hardening. Adults from all species were heat-hardened by exposure to 41 °C for 1 h, subsequently tested for (1) heat survival and knockdown responses following 1-h heat shocks across a range of temperatures up to the lethal limit, (2) ramping CTmax assays and (3) static temperature knockdown time assays. Females from all three species were more heat tolerant than males in the 1-h heat shock assays, but effects of sex were absent or inconsistent for CTmax and heat knockdown time assays. A beneficial impact of heat hardening on subsequent heat shock knockdown was evident in both sexes of all three species. However, hardening effects differed substantially for survival 24 h later, ranging from no effect of hardening in <em>Cx. quinquefasciatus</em> to a ∼1 °C increase in LT₅₀ in <em>Ae. notoscriptus</em>. In contrast, no effects of heat hardening were detected for CTmax or static knockdown time assays. An additional experiment in <em>Ae. aegypti</em> detected no benefits of parental heat shock exposure on the thermal tolerance of offspring. Our findings emphasize the need to consider effects of acclimation including heat hardening in models to predict the response of mosquitoes to climate warming. They also have implications for measuring thermal tolerance more generally, as sex and hardening effects depend on the type of assay used and trait measured.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104394"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Migration phenology of adult Chinook salmon: tradeoffs among acute and cumulative thermal exposure risks","authors":"Matthew L. Keefer,&nbsp;Christopher C. Caudill","doi":"10.1016/j.jtherbio.2026.104388","DOIUrl":"10.1016/j.jtherbio.2026.104388","url":null,"abstract":"<div><div>Migratory coldwater species like anadromous salmonids are highly sensitive to river temperatures and methods are needed for estimating their exposure and risk. We built an agent-based model (ABM) for a threatened spring-run Chinook salmon population over 220-km of their adult migration in Oregon’s Willamette and North Santiam River basins. Upstream movement rules for the ABM were derived from a companion study of 161 radio-tagged salmon that moved through five study reaches before reaching prespawn holding sites. Tagged fish moved faster when river temperatures were warmer in all reaches, informing movement rules. We used the ABM to simulate continuous thermal histories for salmon migrating in two years (2011, 2015) with sharply contrasting thermal regimes and used the simulations to address hypotheses about thermal exposure and prespawn mortality risks. In both cool (2011) and warm (2015) years, early-run salmon had high cumulative exposure, whereas late-run salmon encountered acutely high temperatures, indicating substantive risk trade-offs within annual runs. On average, total degree day (DD) accumulations during migration and holding were ∼1100 (2011) and ∼1500 DD (2015), with 56 % and 69 %, respectively, accruing during holding in the terminal reach. Approximately 3 % (2011) and 24 % (2015) of simulated salmon encountered temperature maxima ≥21 °C, a threshold with known behavioral and physiological impacts. Estimates of prespawn mortality from an independent carcass dataset were ∼29 % (2011) and ∼61 % (2015), but it is unknown whether cumulative or acute processes (or both) precipitated the mortality. ABMs can help fisheries managers quantify environmental exposure, identify risks, and develop temperature mitigation strategies.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104388"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RSK2 facilitates beige fat formation through thermogenic and glycolytic pathways","authors":"Yan Cai ,&nbsp;Jun-jun Chen ,&nbsp;Lin-fei He ,&nbsp;Yao Xu ,&nbsp;Rui-qi Yang ,&nbsp;Zhi-chun Yang","doi":"10.1016/j.jtherbio.2026.104389","DOIUrl":"10.1016/j.jtherbio.2026.104389","url":null,"abstract":"<div><h3>Objectives</h3><div>Beige adipose tissue offers therapeutic potential for metabolic disorders. Notably, glycolytic beige adipocytes, which can be activated independently of β-adrenergic signaling, may provide unique advantages. This study aimed to investigate the role of p90 ribosomal S6 kinase-2 (RSK2) in both cold-induced and cold-independent glycolytic beige adipocyte formation.</div></div><div><h3>Methods</h3><div>We employed C57BL/6J mice subjected to cold exposure (4 °C) or high-fat diet (HFD) and analyzed adipose tissue for thermogenic and glycolytic markers. In vitro, subcutaneous adipose-derived stem cells (primary preadipocytes) were cultured at 28 °C to mimic cold exposure. RSK2 expression was manipulated via knockdown or overexpression to evaluate its functional role.</div></div><div><h3>Results</h3><div>Cold exposure upregulated uncoupling protein-1 (UCP1) and RSK2 in vivo, while HFD suppressed RSK2, UCP1, enolase 1 (ENO1), and pyruvate kinase isoform M2 (PKM2). In primary preadipocytes, cold exposure induced UCP1 and RSK2 expression and reduced triglyceride accumulation. RSK2 knockdown suppressed UCP1, acetyl-CoA carboxylase (ACC), and hormone-sensitive lipase (HSL) but increased carnitine palmitoyl transferase expression. It also reduced PKM2, ENO1, and lactate production under normothermic conditions, whereas RSK2 overexpression had opposite effects. High-glucose and high-insulin treatment decreased RSK2, glycolytic and thermogenic markers, and insulin signaling, which were reversed by RSK2 overexpression. <strong>Conclusions</strong>: RSK2 plays a dual role in mediating cold-induced and cold-independent glycolytic beige adipocyte formation. It may serve as a promising target for treating obesity and related metabolic disorders.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104389"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical simulation study on the thermo-mechanical coupling damage mechanism in microwave ablation of lung tumors","authors":"Yujie Fu,&nbsp;Qun Nan","doi":"10.1016/j.jtherbio.2026.104404","DOIUrl":"10.1016/j.jtherbio.2026.104404","url":null,"abstract":"<div><div>Lung cancer is one of the most threatening malignant tumors globally, and microwave ablation (MWA) serves as a crucial minimally invasive approach for treating early-stage patients who are intolerant to surgery. However, the lung, as a typical porous medium, remains unclear in terms of the correlation mechanism between thermal stress evolution during MWA and postoperative complications such as cavities. This study aims to reveal the thermo-mechanical coupling damage mechanism during lung MWA through numerical simulation. Based on the porous medium theory and the two-phase lag heat transfer model, an electromagnetic-thermal-mechanical multiphysics coupled finite element model was constructed to simulate the complete ablation process of lung tumors using a 2450 MHz microwave antenna. The simulation results indicate the presence of two damage risk zones with distinct mechanical characteristics during MWA: the high-temperature core zone, which is dominated by thermal coagulation necrosis and phase change contraction, bears the highest equivalent stress; whereas the tissue transition zone, due to significant gradients in temperature and material properties, becomes a region where strain first increases and then decreases, accompanied by shear stress concentration, making it a high-risk area for tearing-type damage and potential cavity formation. During the cooling phase, significant redistribution and relaxation of stress occur in both the core and transition zones. The presence of residual strain and stress confirms that irreversible phase change damage is the primary mechanism responsible for the permanent volume reduction of the ablation zone. This study systematically elucidates the “dual risk zone” mechanism of thermo-mechanical coupling damage in lung MWA, reveals the importance of mechanical evolution during the cooling phase, and provides an important theoretical basis for developing precise and safe ablation strategies based on mechanical thresholds.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104404"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146157690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Online decoupled estimation of temperature field in biological tissue during invasive laser thermal therapy","authors":"Zhaohui Mao ,&nbsp;Hong Chen ,&nbsp;Guangjun Wang ,&nbsp;Zhenbiao Li","doi":"10.1016/j.jtherbio.2026.104413","DOIUrl":"10.1016/j.jtherbio.2026.104413","url":null,"abstract":"<div><div>Invasive laser-induced thermal therapy (LITT) is an emerging minimally invasive technique for treating deep-seated tumors. Online estimation of the transient temperature field in target biological tissue is crucial for the research and development of LITT technology. Existing state estimation theories, which employ the state-space model to describe the system, essentially belong to coupled estimation methods. They cannot achieve decoupled estimation of system states, leading to numerous challenges in applying current state estimation methods to temperature field estimation during LITT procedures. This paper focuses on the online decoupled estimation of the temperature field in biological tissue during invasive LITT. By constructing an optimal estimation model and a spatiotemporal correlation model, it achieves online decoupled estimation of temperatures at critical discrete points throughout the invasive LITT process. This scheme effectively overcomes the difficulties inherent in existing temperature field estimation techniques based on state-space models. The reliability of the constructed biological model is validated using existing experimental data. Through numerical simulations, the impacts of the influence time domain, measurement noise, and model mismatch are analyzed. Comparison with the standard Kalman filter demonstrates that when only real-time temperature estimation of specific regions (such as tumor regions) is required, the decoupled estimation scheme proposed in this paper exhibits significant advantages in computational efficiency. This research provides reliable technical support for the online estimation of tissue temperature fields.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104413"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-dependent calcium dynamics in mouse skeletal muscle: Responses to cooling and caffeine","authors":"Kosei Hayakawa ,&nbsp;Ryo Takagi ,&nbsp;Ayaka Tabuchi ,&nbsp;Haruka Ugawa ,&nbsp;Daiki Watanabe ,&nbsp;Daisuke Hoshino ,&nbsp;David C. Poole ,&nbsp;Yutaka Kano","doi":"10.1016/j.jtherbio.2026.104412","DOIUrl":"10.1016/j.jtherbio.2026.104412","url":null,"abstract":"<div><div>Intracellular calcium ion concentration ([Ca²⁺]i) regulation in skeletal muscle may vary with sex and muscle fiber type, but the precise nature of its response to temperature changes and pharmacological caffeine stimulation is not fully understood. This study aimed to elucidate sex-dependent and muscle fiber type-specific characteristics of muscle cooling, caffeine stimulation, and their combined effects. We investigated the effects of cooling (30 °C to 0 °C) and caffeine stimulation (1.25–80 mM) separately and in combination (cooling + 1.25 mM caffeine) in fast-twitch (plantaris, PLA) and slow-twitch (soleus, SOL) muscles of male and female C57BL/6J mice. [Ca²⁺]i dynamics were analyzed using <em>in vivo</em> Fura-2 bioimaging under isoflurane anesthesia. The temperature threshold for the onset of [Ca²⁺]i accumulation was significantly higher in SOL than in PLA, with no significant difference between sexes (males: PLA 2.3 ± 0.9 °C, SOL 4.5 ± 2.2 °C; females: PLA 2.3 ± 0.8 °C, SOL 4.3 ± 1.3 °C). Conversely, the [Ca²⁺]i response to caffeine was significantly higher in females than in males at high concentrations (80 mM). Furthermore, the combined stimulation of cooling and caffeine had a greater effect on females than on males. Our findings also indicate that the phosphorylation response of ryanodine receptors to caffeine was significantly higher in females than in males. In conclusion, while no sex differences were observed in the [Ca²⁺]i response to cooling, clear sex-dependent differences (males &lt; females) were observed in the response to caffeine.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104412"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}