Journal of thermal biology最新文献_第2页

Deciphering the molecular mechanisms of heat stress tolerance in goats: Insights from transcriptome and Gene Co-expression analysis 解密山羊耐热胁迫的分子机制：转录组和基因共表达分析的启示。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2024-10-01 DOI: 10.1016/j.jtherbio.2024.104007

Mahesh Shivanand Dige , Ankita Gurao , Arnav Mehrotra , Manoj Kumar Singh , Amit Kumar , Rakesh Kaushik , Ranjit Singh Kataria , Pramod Kumar Rout

Climate change poses a significant threat to the sustainability of livestock production systems in developing countries, particularly impacting small ruminants like goats, which are highly susceptible to heat stress. This stressor not only reduces productivity but also undermines economic viability. This study aimed to delve into the molecular mechanisms underlying heat stress tolerance in goats by conducting a comprehensive transcriptome analysis of heat-tolerant (HT, n = 4) and heat-susceptible (HS, n = 6) Jamunapari goats. Physiological metrics, such as rectal temperature, respiratory rate, and heart rate, were meticulously monitored under extreme environmental conditions (Temperature Humidity Index >92) to effectively classify goats based on their distinct heat stress responses. Samples of blood were obtained, and peripheral blood mononuclear cells (PBMCs) were extracted for subsequent RNA extraction. RNA-Seq analysis revealed a sum of 734 differentially expressed genes (DEGs), comprising 251 upregulated and 483 downregulated genes in HT goats compared to their HS counterparts. The WGCNA revealed three key modules, darkorange (tolerance), paleturquoise (respiration rate), and darkmagenta (heart rate). Moreover, functional enrichment analysis revealed that DEGs within these modules played intricate roles in crucial biological processes and pathways, including mitochondrial function, cardiac function, immune response, genomic stability, and metabolic regulation. This research notably enhances our comprehension of the genetic underpinnings of thermo-tolerance in goats and provides invaluable guidance for formulating breeding strategies aimed at bolstering livestock resilience against the challenges of climate change.

气候变化对发展中国家畜牧生产系统的可持续性构成重大威胁，尤其影响到山羊等小型反刍动物，因为它们极易受到热应激的影响。这种胁迫不仅会降低生产力，还会破坏经济可行性。本研究旨在通过对耐热山羊（HT，n = 4）和热敏感山羊（HS，n = 6）进行全面的转录组分析，深入研究山羊耐热应激的分子机制。在极端环境条件下（温湿度指数大于 92），对直肠温度、呼吸频率和心率等生理指标进行了细致监测，以便根据山羊不同的热应激反应对其进行有效分类。采集血液样本并提取外周血单核细胞（PBMCs），然后提取 RNA。RNA-Seq分析显示，与HS山羊相比，HT山羊共有734个差异表达基因（DEGs），包括251个上调基因和483个下调基因。WGCNA揭示了三个关键模块：深橙色（耐受性）、浅绿色（呼吸速率）和深品红色（心率）。此外，功能富集分析表明，这些模块中的 DEGs 在线粒体功能、心脏功能、免疫反应、基因组稳定性和代谢调控等关键生物过程和途径中发挥着复杂的作用。这项研究显著提高了我们对山羊耐热性遗传基础的理解，并为制定育种策略提供了宝贵的指导，这些育种策略旨在提高牲畜应对气候变化挑战的能力。

{"title":"Deciphering the molecular mechanisms of heat stress tolerance in goats: Insights from transcriptome and Gene Co-expression analysis","authors":"Mahesh Shivanand Dige , Ankita Gurao , Arnav Mehrotra , Manoj Kumar Singh , Amit Kumar , Rakesh Kaushik , Ranjit Singh Kataria , Pramod Kumar Rout","doi":"10.1016/j.jtherbio.2024.104007","DOIUrl":"10.1016/j.jtherbio.2024.104007","url":null,"abstract":"<div><div>Climate change poses a significant threat to the sustainability of livestock production systems in developing countries, particularly impacting small ruminants like goats, which are highly susceptible to heat stress. This stressor not only reduces productivity but also undermines economic viability. This study aimed to delve into the molecular mechanisms underlying heat stress tolerance in goats by conducting a comprehensive transcriptome analysis of heat-tolerant (HT, n = 4) and heat-susceptible (HS, n = 6) Jamunapari goats. Physiological metrics, such as rectal temperature, respiratory rate, and heart rate, were meticulously monitored under extreme environmental conditions (Temperature Humidity Index >92) to effectively classify goats based on their distinct heat stress responses. Samples of blood were obtained, and peripheral blood mononuclear cells (PBMCs) were extracted for subsequent RNA extraction. RNA-Seq analysis revealed a sum of 734 differentially expressed genes (DEGs), comprising 251 upregulated and 483 downregulated genes in HT goats compared to their HS counterparts. The WGCNA revealed three key modules, darkorange (tolerance), paleturquoise (respiration rate), and darkmagenta (heart rate). Moreover, functional enrichment analysis revealed that DEGs within these modules played intricate roles in crucial biological processes and pathways, including mitochondrial function, cardiac function, immune response, genomic stability, and metabolic regulation. This research notably enhances our comprehension of the genetic underpinnings of thermo-tolerance in goats and provides invaluable guidance for formulating breeding strategies aimed at bolstering livestock resilience against the challenges of climate change.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster 热胁迫对黑腹果蝇精子发生和卵子生成的影响具有生命阶段特异性

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2024-10-01 DOI: 10.1016/j.jtherbio.2024.104001

Abhishek Meena, Komal Maggu, Alessio N. De Nardo, Sonja H. Sbilordo, Benjamin Eggs, Rawaa Al Toma Sho, Stefan Lüpold

Biodiversity is increasingly threatened by unpredictable, frequent, and intense climatic events like heatwaves that pose harmful impacts on ectotherms. Beyond the health and survival of organisms, reduced reproductive performance has emerged as a critical fitness consequence of thermal stress induced by high temperatures. Many studies on these effects expose organisms to heat stress during the adult stage or throughout development, often focusing on cumulative effects across life stages, and they tend to examine one or the other sex. This approach may not reflect the short-term nature of many extreme heat events and limits our understanding of stage- and sex-specific fitness consequences in short-lived organisms. To address this gap, we used Drosophila melanogaster to investigate the sex-specific reproductive performance following short heat stress of varying intensity at different developmental stages. We found the thermal sensitivity to be higher in males than females, and to increase toward adult emergence, leading to nearly complete reproductive failure and substantially slowed recovery. These results highlight how even brief bouts of heat stress during a sensitive phase could affect population dynamics and persistence. Our findings also underscore that incorporating both sex and life stage could improve predictions of species persistence.

热浪等不可预测、频繁和强烈的气候事件给外温动物造成了有害影响，生物多样性正日益受到威胁。除了生物的健康和存活之外，高温引起的热应激还导致生殖能力下降，这是一个重要的健康后果。许多有关这些影响的研究都将生物暴露于成体阶段或整个发育过程中的热应激，通常侧重于各生命阶段的累积影响，而且这些研究往往只考察一种或另一种性别。这种方法可能无法反映许多极端高温事件的短期性质，并限制了我们对短寿命生物的阶段和性别特异性适应性后果的了解。为了弥补这一不足，我们利用黑腹果蝇研究了不同发育阶段的性别在不同强度的短期热胁迫下的生殖表现。我们发现雄果蝇的热敏感性高于雌果蝇，而且在成虫萌发过程中，雄果蝇的热敏感性增加，导致生殖几乎完全失败，恢复速度大大减慢。这些结果突显了在敏感期即使是短暂的热应激也会影响种群动态和持续性。我们的研究结果还强调，将性别和生命阶段结合起来可以改善对物种持续性的预测。

{"title":"Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster","authors":"Abhishek Meena, Komal Maggu, Alessio N. De Nardo, Sonja H. Sbilordo, Benjamin Eggs, Rawaa Al Toma Sho, Stefan Lüpold","doi":"10.1016/j.jtherbio.2024.104001","DOIUrl":"10.1016/j.jtherbio.2024.104001","url":null,"abstract":"<div><div>Biodiversity is increasingly threatened by unpredictable, frequent, and intense climatic events like heatwaves that pose harmful impacts on ectotherms. Beyond the health and survival of organisms, reduced reproductive performance has emerged as a critical fitness consequence of thermal stress induced by high temperatures. Many studies on these effects expose organisms to heat stress during the adult stage or throughout development, often focusing on cumulative effects across life stages, and they tend to examine one or the other sex. This approach may not reflect the short-term nature of many extreme heat events and limits our understanding of stage- and sex-specific fitness consequences in short-lived organisms. To address this gap, we used <em>Drosophila melanogaster</em> to investigate the sex-specific reproductive performance following short heat stress of varying intensity at different developmental stages. We found the thermal sensitivity to be higher in males than females, and to increase toward adult emergence, leading to nearly complete reproductive failure and substantially slowed recovery. These results highlight how even brief bouts of heat stress during a sensitive phase could affect population dynamics and persistence. Our findings also underscore that incorporating both sex and life stage could improve predictions of species persistence.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Five weeks of intermittent hand exposures to alternating cold and hot stimuli do not modify finger cold-induced vasodilatation response 手部间歇接触冷热交替刺激五周不会改变手指冷引起的血管扩张反应。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2024-10-01 DOI: 10.1016/j.jtherbio.2024.104004

Roger Kölegård , Lena Norrbrand , Ola Eiken , Michail E. Keramidas

We tested the hypothesis that prolonged intermittent hand exposures to transient contrast thermal stimuli would enhance the finger cold-induced vasodilatation (CIVD) response during localized cooling. Eight healthy men participated in a 5-week regimen, during which they immersed, thrice per week, the non-dominant (EXP) hand in 8° and 43 °C water, sequentially and at 3-min intervals, for a total period of 60 min. The contralateral (i.e., dominant) hand served as the control (CON) hand. Before and after the regimen, subjects conducted two 30-min hand cold (8 °C water) provocation trials, one with the EXP hand and the other with the CON hand. In addition, a flow-mediated dilatation test was performed in the brachial artery of the EXP arm. Regardless of the hand tested, the average finger skin temperature [CON hand: pre-trial = 10.5 (1.2)°C, post-trial = 10.8 (1.3)°C and EXP hand: pre-trial = 10.7 (1.1)°C, post-trial 10.9 (1.1)°C; p = 0.79], and the incidence of CIVD events [CON hand: pre-trial = 1.1 (1.2) events, post-trial = 1.2 (1.1) events and EXP hand: pre-trial = 1.1 (0.8) events, post-trial = 1.1 (0.8) events; p = 0.88] were not affected by the 5-week regimen. The sensation of cold-induced pain was transiently alleviated following the regimen (p = 0.02). The flow-mediated dilatation response of the EXP brachial artery remained unaltered [pre-trial = 5.4 (3.2)%, post-trial = 4.7 (3.6)%; p = 0.51]. Therefore, five weeks of intermittent hand exposures to alternating cold and hot stimuli do not improve finger temperature responsiveness to sustained localized cold.

我们测试了这样一个假设：手部长时间间歇性暴露于瞬时对比热刺激会增强手指在局部冷却过程中的冷诱导血管扩张（CIVD）反应。八名健康男性参加了为期五周的疗程，在此期间，他们每周三次将非惯用（EXP）手依次浸入 8° 和 43°C 的水中，每次间隔 3 分钟，共 60 分钟。对侧（即优势）手作为对照（CON）手。在疗程前后，受试者分别用 EXP 手和 CON 手进行了两次长达 30 分钟的手冷（8 °C 水）刺激试验。此外，还对 EXP 手臂的肱动脉进行了血流介导的扩张试验。无论用哪只手测试，平均手指皮肤温度[CON 手：试验前 = 10.5 (1.2)°C，试验后 = 10.8 (1.3)°C；EXP 手：试验前 = 10.7 (1.1)°C，试验后 10.9 (1.1)° C；p = 0.79]，CIVD 事件的发生率[CON 手：试验前 = 1.1 (1.2) 事件，试验后 = 1.2 (1.1) 事件和 EXP 手：试验前 = 1.1 (0.8) 事件，试验后 = 1.1 (0.8) 事件；p = 0.88]不受 5 周治疗方案的影响。疗程结束后，冷引起的疼痛感得到短暂缓解（p = 0.02）。外周肱动脉的血流介导扩张反应保持不变[试验前 = 5.4 (3.2)%，试验后 = 4.7 (3.6)%；p = 0.51]。因此，手部间歇性暴露于冷热交替刺激五周并不能改善手指对持续局部冷的温度反应。

{"title":"Five weeks of intermittent hand exposures to alternating cold and hot stimuli do not modify finger cold-induced vasodilatation response","authors":"Roger Kölegård , Lena Norrbrand , Ola Eiken , Michail E. Keramidas","doi":"10.1016/j.jtherbio.2024.104004","DOIUrl":"10.1016/j.jtherbio.2024.104004","url":null,"abstract":"<div><div>We tested the hypothesis that prolonged intermittent hand exposures to transient contrast thermal stimuli would enhance the finger cold-induced vasodilatation (CIVD) response during localized cooling. Eight healthy men participated in a 5-week regimen, during which they immersed, thrice per week, the non-dominant (EXP) hand in 8° and 43 °C water, sequentially and at 3-min intervals, for a total period of 60 min. The contralateral (i.e., dominant) hand served as the control (CON) hand. Before and after the regimen, subjects conducted two 30-min hand cold (8 °C water) provocation trials, one with the EXP hand and the other with the CON hand. In addition, a flow-mediated dilatation test was performed in the brachial artery of the EXP arm. Regardless of the hand tested, the average finger skin temperature [CON hand: pre-trial = 10.5 (1.2)°C, post-trial = 10.8 (1.3)°C and EXP hand: pre-trial = 10.7 (1.1)°C, post-trial 10.9 (1.1)°C; <em>p</em> = 0.79], and the incidence of CIVD events [CON hand: pre-trial = 1.1 (1.2) events, post-trial = 1.2 (1.1) events and EXP hand: pre-trial = 1.1 (0.8) events, post-trial = 1.1 (0.8) events; <em>p</em> = 0.88] were not affected by the 5-week regimen. The sensation of cold-induced pain was transiently alleviated following the regimen (<em>p</em> = 0.02). The flow-mediated dilatation response of the EXP brachial artery remained unaltered [pre-trial = 5.4 (3.2)%, post-trial = 4.7 (3.6)%; <em>p</em> = 0.51]. Therefore, five weeks of intermittent hand exposures to alternating cold and hot stimuli do not improve finger temperature responsiveness to sustained localized cold.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of acute mild winter conditions on the productivity of feedlot cattle: An Australian perspective 冬季极端温和条件对饲养场牛生产率的影响：澳大利亚的视角。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2024-10-01 DOI: 10.1016/j.jtherbio.2024.103989

Pippa J. Pryor , Billie Standfield , Janelle Wilkes, Léa Labeur , Angela M. Lees

The impact of cold stress on feedlot cattle has received limited investigation in temperate climates. However, cold stress has been found to impact cattle welfare and production in temperate climatic conditions, where ambient temperature (T_A), precipitation, relative humidity (RH), wind speed (WS) and solar radiation (SR) all influencing apparent temperature. While there are many indices to quantify cold stress in cattle, there are limited indices that directly relate to cattle and account for all the interactions between climatic variables. This makes cold stress difficult to quantify, with on-cattle measures such as core body temperature and shivering difficult metrics to obtain in feedlot cattle. Moreover, individual cattle will react differently to cold conditions based on their production, age, sex, coat characteristics (colour and type), breed and nutrition. While cold stress can be difficult to measure, the production status, behavioural and physiological effects of cold stress have been identified, but generally under what could be considered as extreme cold stress events. Similarly, while mitigation strategies such as shelter, bedding and windbreaks have been investigated, limited information is available surrounding their usefulness in temperate climates. Further research is needed to fully investigate the effects of cold stress and how to mitigate those effects in feedlot cattle under conditions, such as those found in Australia.

在温带气候条件下，冷应激对饲养场牛只影响的研究十分有限。然而，在温带气候条件下，环境温度（TA）、降水量、相对湿度（RH）、风速（WS）和太阳辐射（SR）都会影响表观温度，因此冷应激会影响牛的福利和生产。虽然有许多指数可以量化牛的冷应激，但与牛直接相关并考虑到气候变量之间所有相互作用的指数却很有限。这使得冷应激难以量化，在饲养场中很难获得牛的核心体温和颤抖等牛体测量指标。此外，每头牛会根据其产量、年龄、性别、被毛特征（颜色和类型）、品种和营养状况对寒冷条件做出不同的反应。虽然冷应激难以测量，但冷应激对生产状况、行为和生理的影响已被确定，但一般是在可被视为极端冷应激事件的情况下。同样，虽然已经对遮蔽物、垫料和防风林等缓解策略进行了研究，但有关这些策略在温带气候条件下的作用的信息十分有限。需要进一步开展研究，以全面调查冷应激的影响，以及如何在澳大利亚等地的条件下减轻饲养场牛的这些影响。

{"title":"Influence of acute mild winter conditions on the productivity of feedlot cattle: An Australian perspective","authors":"Pippa J. Pryor , Billie Standfield , Janelle Wilkes, Léa Labeur , Angela M. Lees","doi":"10.1016/j.jtherbio.2024.103989","DOIUrl":"10.1016/j.jtherbio.2024.103989","url":null,"abstract":"<div><div>The impact of cold stress on feedlot cattle has received limited investigation in temperate climates. However, cold stress has been found to impact cattle welfare and production in temperate climatic conditions, where ambient temperature (T<sub>A</sub>), precipitation, relative humidity (RH), wind speed (WS) and solar radiation (SR) all influencing apparent temperature. While there are many indices to quantify cold stress in cattle, there are limited indices that directly relate to cattle and account for all the interactions between climatic variables. This makes cold stress difficult to quantify, with on-cattle measures such as core body temperature and shivering difficult metrics to obtain in feedlot cattle. Moreover, individual cattle will react differently to cold conditions based on their production, age, sex, coat characteristics (colour and type), breed and nutrition. While cold stress can be difficult to measure, the production status, behavioural and physiological effects of cold stress have been identified, but generally under what could be considered as extreme cold stress events. Similarly, while mitigation strategies such as shelter, bedding and windbreaks have been investigated, limited information is available surrounding their usefulness in temperate climates. Further research is needed to fully investigate the effects of cold stress and how to mitigate those effects in feedlot cattle under conditions, such as those found in Australia.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patterns of thermal normality of the knee joint in professional athletes of different sports 不同运动项目职业运动员膝关节的热正常模式。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2024-10-01 DOI: 10.1016/j.jtherbio.2024.103991

Cristiane Mara de Rezende , Alisson Gomes da Silva , Manuel Sillero-Quintana , João Carlos Bouzas Marins

引用次数: 0

Temperature as an effective biosecurity tool against invasive snakes 温度作为一种有效的生物安全工具，可有效防止蛇类入侵。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2024-10-01 DOI: 10.1016/j.jtherbio.2024.103978

Julien C. Piquet , Borja Maestresalas , Marta López-Darias

The application of thermal treatments could serve as a biosecurity tool to avert snake introduction, which often leads to irreversible ecological impacts. Here, we tested the applicability of conductive heating and thermal fumigation to prevent the spread of the California kingsnake (Lampropeltis californiae), a damaging species established on the island of Gran Canaria, likely to reach other vulnerable regions, and included among the most concerning invasive species of the European Union. We exposed 24 individuals to a thermal gradient to determine species selected temperatures, range of preferred temperatures and voluntary thermal maximum and used other 24 individuals to analyse their response to conductive heating, thermal fumigation and control (no heat) treatments. Lampropeltis californiae selected temperatures of 27.80 ± 1.05 °C (ranging 26.86 °C ± 1.21 °C to 28.68 °C ± 1.25 °C) and a voluntary thermal maximum of 32.50 ± 3.69 °C. Conductive heating and thermal fumigation performed equally well, inducing the exit of 83.33% and 91.67% of all individuals after 14.36 ± 9.25 min and 11.13 ± 8.60 min of exposition, and at a body temperature of 35.66 ± 3.53 °C and 35.57 ± 2.41 °C, respectively. Control treatments produced the exit of 29.17% of all individuals, which came out of the box in 24.80 ± 8.83 min and at a body temperature of 28.60 ± 1.38 °C. Thermal treatments could serve as an effective tool to prevent the inadvertent transportation of L. californiae and other invasive snakes threatening numerous regions around the globe.

蛇类传入往往会对生态造成不可逆转的影响，而热处理可作为一种生物安全工具，避免蛇类传入。在这里，我们测试了传导加热和热熏蒸在防止加州王蛇（Lampropeltis californiae）传播方面的适用性，加州王蛇是大加那利岛的一种有害物种，很可能会传播到其他脆弱地区，并被列为欧盟最令人担忧的入侵物种之一。我们将 24 个个体暴露在热梯度下，以确定物种选择的温度、偏好的温度范围和自愿的最高温度，并利用其他 24 个个体分析它们对传导加热、热熏蒸和对照（无热）处理的反应。Lampropeltis californiae选择的温度为27.80 ± 1.05 °C（范围为26.86 ° C ± 1.21 °C至28.68 ° C ± 1.25 °C），自愿最高温度为32.50 ± 3.69 °C。传导加热和热熏蒸的效果相同，在暴露 14.36 ± 9.25 分钟和 11.13 ± 8.60 分钟后，分别有 83.33% 和 91.67% 的个体退出，体温分别为 35.66 ± 3.53 °C 和 35.57 ± 2.41 °C。对照组有 29.17% 的个体在 24.80 ± 8.83 分钟内出箱，体温为 28.60 ± 1.38 °C。热处理可作为一种有效工具，防止加利福尼亚蛇和其他入侵蛇的意外运输，这些蛇威胁着全球许多地区。

{"title":"Temperature as an effective biosecurity tool against invasive snakes","authors":"Julien C. Piquet , Borja Maestresalas , Marta López-Darias","doi":"10.1016/j.jtherbio.2024.103978","DOIUrl":"10.1016/j.jtherbio.2024.103978","url":null,"abstract":"<div><div>The application of thermal treatments could serve as a biosecurity tool to avert snake introduction, which often leads to irreversible ecological impacts. Here, we tested the applicability of conductive heating and thermal fumigation to prevent the spread of the California kingsnake (<em>Lampropeltis californiae</em>), a damaging species established on the island of Gran Canaria, likely to reach other vulnerable regions, and included among the most concerning invasive species of the European Union. We exposed 24 individuals to a thermal gradient to determine species selected temperatures, range of preferred temperatures and voluntary thermal maximum and used other 24 individuals to analyse their response to conductive heating, thermal fumigation and control (no heat) treatments. <em>Lampropeltis californiae</em> selected temperatures of 27.80 ± 1.05 °C (ranging 26.86 °C ± 1.21 °C to 28.68 °C ± 1.25 °C) and a voluntary thermal maximum of 32.50 ± 3.69 °C. Conductive heating and thermal fumigation performed equally well, inducing the exit of 83.33% and 91.67% of all individuals after 14.36 ± 9.25 min and 11.13 ± 8.60 min of exposition, and at a body temperature of 35.66 ± 3.53 °C and 35.57 ± 2.41 °C, respectively. Control treatments produced the exit of 29.17% of all individuals, which came out of the box in 24.80 ± 8.83 min and at a body temperature of 28.60 ± 1.38 °C. Thermal treatments could serve as an effective tool to prevent the inadvertent transportation of <em>L. californiae</em> and other invasive snakes threatening numerous regions around the globe.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive capability of slow-growing backyard poultry as indicated by physiological and molecular responses in a hot and humid coastal climate 湿热沿海气候下散养家禽生理和分子反应所显示的缓慢生长的适应能力。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2024-10-01 DOI: 10.1016/j.jtherbio.2024.103985

Nibedita Nayak , Subrat Kumar Bhanja , Eaknath B. Chakurkar , Amiya Ranjan Sahu

Assessing the adaptability of slow-growing rural chickens for improving thermotolerance to suit the global climate change is a major research need. This work was aimed to evaluate the adaptability of CARI-Debendra chickens and to identify the polymorphism as well as expression profiling of thermotolerant genes (HSP70 and GRP78) under prevailing temperature-humidity indices and thermal stress in a coastal environment. One hundred sixty straight run chicks were reared at THI≥75 (control) and THI>80 under coastal climate till 12 weeks. Polymorphism of HSP70 and GRP78 candidate genes were explored using restriction enzymes TaqI and HaeIII to identify possible thermotolerance markers. Expression profiling of both the genes in liver, intestine and pectoralis muscle was determined through quantitative real-time PCR. Rectal and body surface temperature recorded in the neck and back showed significant differences (P < 0.01) with higher temperature in THI>80 group. Comparatively lower live weights (P < 0.05) and poor FCR were recorded in THI>80 group. The villi height in all intestinal segments was significantly lower (P < 0.01), but deeper crypt depth was observed in THI>80 than control group. A lowest thymus weight (P < 0.05) was noted with no significant differences in immune response in treatment group. Serum levels of cholesterol, activities of lactate dehydrogenase, creatinine kinase and concentration of potassium, sodium and thyroxine hormone were not different between the 2 groups. The concentration of triiodothyronine and chloride ion was lower in THI>80 group indicating adaptive changes for thermoregulation. HSP70 gene expressions in the three tissues were differentially increased (P < 0.01) by temperature-humidity indices, but the expression of GRP78 was not different between the 2 groups. The results concluded that the environmental factors interact with genetics on adaptability towards thermotolerance in slow-growing chickens.

评估农村慢生鸡的适应性以提高耐热性，从而适应全球气候变化是一项重大的研究需求。本研究旨在评估 CARI-Debendra 鸡的适应性，并确定耐热基因（HSP70 和 GRP78）的多态性，以及在沿海环境的主要温湿度指数和热应力下的表达谱。在THI≥75（对照组）和THI>80的沿海气候条件下饲养160只直立雏鸡至12周。使用限制性酶 TaqI 和 HaeIII 研究了 HSP70 和 GRP78 候选基因的多态性，以确定可能的耐热性标记。通过定量实时聚合酶链式反应测定了这两个基因在肝脏、肠道和胸肌中的表达谱。颈部和背部记录的直肠温度和体表温度显示出显著差异（P 80）。活体重相对较低（P 80 组）。所有肠段的绒毛高度都明显低于对照组（P 80）。胸腺重量最低（P 80 组），表明体温调节发生了适应性变化。三种组织中的 HSP70 基因表达量均有不同程度的增加（P

{"title":"Adaptive capability of slow-growing backyard poultry as indicated by physiological and molecular responses in a hot and humid coastal climate","authors":"Nibedita Nayak , Subrat Kumar Bhanja , Eaknath B. Chakurkar , Amiya Ranjan Sahu","doi":"10.1016/j.jtherbio.2024.103985","DOIUrl":"10.1016/j.jtherbio.2024.103985","url":null,"abstract":"<div><div>Assessing the adaptability of slow-growing rural chickens for improving thermotolerance to suit the global climate change is a major research need. This work was aimed to evaluate the adaptability of CARI-Debendra chickens and to identify the polymorphism as well as expression profiling of thermotolerant genes (HSP70 and GRP78) under prevailing temperature-humidity indices and thermal stress in a coastal environment. One hundred sixty straight run chicks were reared at THI≥75 (control) and THI>80 under coastal climate till 12 weeks. Polymorphism of HSP70 and GRP78 candidate genes were explored using restriction enzymes TaqI and HaeIII to identify possible thermotolerance markers. Expression profiling of both the genes in liver, intestine and pectoralis muscle was determined through quantitative real-time PCR. Rectal and body surface temperature recorded in the neck and back showed significant differences (P < 0.01) with higher temperature in THI>80 group. Comparatively lower live weights (P < 0.05) and poor FCR were recorded in THI>80 group. The villi height in all intestinal segments was significantly lower (P < 0.01), but deeper crypt depth was observed in THI>80 than control group. A lowest thymus weight (P < 0.05) was noted with no significant differences in immune response in treatment group. Serum levels of cholesterol, activities of lactate dehydrogenase, creatinine kinase and concentration of potassium, sodium and thyroxine hormone were not different between the 2 groups. The concentration of triiodothyronine and chloride ion was lower in THI>80 group indicating adaptive changes for thermoregulation. HSP70 gene expressions in the three tissues were differentially increased (P < 0.01) by temperature-humidity indices, but the expression of GRP78 was not different between the 2 groups. The results concluded that the environmental factors interact with genetics on adaptability towards thermotolerance in slow-growing chickens.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiac electrical and functional activity following an outdoor cold-water swimming event 户外冷水游泳后的心电和功能活动。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2024-10-01 DOI: 10.1016/j.jtherbio.2024.103996

Victorien Faivre-Rampant , Corentin Hingrand , A. Mezanger , Eric Saloux , Pierre Ollitrault , Simon Alvado , Hervé Normand , Igor B. Mekjavic , Thibaud Collet , Benoit Mauvieux , Joffrey Drigny , Amir Hodzic

Aims

Participation in outdoor cold-water swimming (OCWS) events combines endurance exercise and cold exposure. Concerns have emerged about the potential risk of acute adverse cardiac events during OCWS, particularly during endurance events. We analysed the effect of prolonged OCWS on cardiac function in trained athletes.

Methods

The swimming event consisted of laps over a 1000-m course, for up to 6 h, in water at 15 °C. Twenty participants (11 males, 47.3 ± 8.6 years old) were included. Core temperature (T_core) was monitored using an ingestible temperature sensor during and up to 1 h after the swim. Body composition, blood pressure, electrocardiogram (ECG), and transthoracic echocardiography were assessed 1 day before the event and within the first hour upon completion of the swim.

Results

Mean body mass index was 27.1 ± 5.1 kg/m² and fat mass was 25.2 ± 9.1 %. Mean duration of swimming was 214 ± 115 min. Minimum Tcore was 35.6 ± 1.3 °C. A significant lengthening of the QT interval corrected (QTc) for heart rate was observed post-exercise (437.7 ± 27.7 vs. 457.2 ± 35.9 ms, p = 0.012), with 5 participants exhibiting post-exercise QTc >500ms. OCWS did not alter the biventricular systolic function and left ventricular relaxation. No correlation was observed between ΔQTc and ΔTcore.

Conclusion

OCWS seemed to acutely delay post-exercise cardiac repolarization without alteration of cardiac function in a healthy trained population. Additional investigations would be warranted to explore the clinical implications of QT lengthening and its relationship with autonomic nervous system regulation during OCWS.

目的：参加室外冷水游泳（OCWS）活动将耐力锻炼和寒冷暴露结合在一起。人们开始担心在室外冷水游泳过程中，尤其是在耐力项目中发生急性不良心脏事件的潜在风险。我们分析了长时间 OCWS 对训练有素的运动员心脏功能的影响：游泳比赛包括在 15 °C的水中在 1000 米的赛道上游泳 6 小时。20名参赛者（11名男性，47.3±8.6岁）参加了比赛。在游泳过程中和游泳后 1 小时内，使用可摄入温度传感器监测核心温度（Tcore）。在比赛前一天和游泳结束后一小时内，对身体成分、血压、心电图和经胸超声心动图进行了评估：平均体重指数为 27.1 ± 5.1 kg/m2，脂肪含量为 25.2 ± 9.1%。平均游泳时间为 214 ± 115 分钟。最低温度为 35.6 ± 1.3 °C。运动后观察到心率校正后的 QT 间期（QTc）明显延长（437.7 ± 27.7 vs. 457.2 ± 35.9 ms，p = 0.012），其中 5 名参与者运动后 QTc >500ms。OCWS 没有改变双心室收缩功能和左心室舒张功能。ΔQTc与ΔTcore之间没有相关性：结论：在健康的训练人群中，OCWS 似乎能急性延迟运动后心脏复极化，但不会改变心脏功能。有必要进行更多研究，以探讨 QT 延长的临床意义及其与 OCWS 期间自律神经系统调节的关系。

{"title":"Cardiac electrical and functional activity following an outdoor cold-water swimming event","authors":"Victorien Faivre-Rampant , Corentin Hingrand , A. Mezanger , Eric Saloux , Pierre Ollitrault , Simon Alvado , Hervé Normand , Igor B. Mekjavic , Thibaud Collet , Benoit Mauvieux , Joffrey Drigny , Amir Hodzic","doi":"10.1016/j.jtherbio.2024.103996","DOIUrl":"10.1016/j.jtherbio.2024.103996","url":null,"abstract":"<div><h3>Aims</h3><div>Participation in outdoor cold-water swimming (OCWS) events combines endurance exercise and cold exposure. Concerns have emerged about the potential risk of acute adverse cardiac events during OCWS, particularly during endurance events. We analysed the effect of prolonged OCWS on cardiac function in trained athletes.</div></div><div><h3>Methods</h3><div>The swimming event consisted of laps over a 1000-m course, for up to 6 h, in water at 15 °C. Twenty participants (11 males, 47.3 ± 8.6 years old) were included. Core temperature (T<sub>core</sub>) was monitored using an ingestible temperature sensor during and up to 1 h after the swim. Body composition, blood pressure, electrocardiogram (ECG), and transthoracic echocardiography were assessed 1 day before the event and within the first hour upon completion of the swim.</div></div><div><h3>Results</h3><div>Mean body mass index was 27.1 ± 5.1 kg/m<sup>2</sup> and fat mass was 25.2 ± 9.1 %. Mean duration of swimming was 214 ± 115 min. Minimum Tcore was 35.6 ± 1.3 °C. A significant lengthening of the QT interval corrected (QTc) for heart rate was observed post-exercise (437.7 ± 27.7 vs. 457.2 ± 35.9 ms, p = 0.012), with 5 participants exhibiting post-exercise QTc >500ms. OCWS did not alter the biventricular systolic function and left ventricular relaxation. No correlation was observed between ΔQTc and ΔTcore.</div></div><div><h3>Conclusion</h3><div>OCWS seemed to acutely delay post-exercise cardiac repolarization without alteration of cardiac function in a healthy trained population. Additional investigations would be warranted to explore the clinical implications of QT lengthening and its relationship with autonomic nervous system regulation during OCWS.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plastic use as nesting material can alter incubation temperature and behaviour but does not affect yellow-legged gull chicks 使用塑料作为筑巢材料会改变孵化温度和行为，但不会影响黄腿鸥雏鸟

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2024-10-01 DOI: 10.1016/j.jtherbio.2024.104005

Sara N. Veríssimo , Filipe Veloso , Francisco Neves , Jaime A. Ramos , Vitor H. Paiva , Ana C. Norte

Optimal incubation temperature is crucial for embryos' development and survival. With the increasing use of plastics in gulls' nests, it is essential to understand how their incorporation affects incubation temperature, parental behaviour, and hatching success. Considering this, we conducted an experiment where plastic was introduced into yellow-legged gulls (Larus michahellis) nests. The experiment comprised three groups: a control group, a group with low amount of plastic, and a third with a high amount of plastic. This design allowed us to investigate the effects of plastic on 1) the heart rate of incubating adults, 2) the number and duration of adults' absences from their nest, 3) how the presence or absence of the adult influenced egg temperature, and 4) chick hatching success, physiological parameters, and bill colour phenotype. We observed that incubation temperature was consistently higher in nests with plastic. The number of absences was higher in the low plastic group at increased temperatures, though the duration was significantly lower in both plastic groups than in the control, possibly to mitigate the effects of heat stress. During higher environmental temperatures, heart rate was higher for the high plastic group. The increase in heart rate in the low plastic group was less pronounced with increasing environmental temperatures. No significant effects were observed on hatching success or in the health condition of young chicks, except for high values of haemoglobin in both plastic groups, which might indicate dehydration.

最佳孵化温度对胚胎的发育和存活至关重要。随着塑料在海鸥巢中的使用越来越多，了解塑料对孵化温度、亲鸟行为和孵化成功率的影响至关重要。有鉴于此，我们进行了一项将塑料引入黄腿鸥（Larus michahellis）巢穴的实验。实验包括三组：对照组、低塑料含量组和高塑料含量组。这种设计使我们能够研究塑料对以下方面的影响：1）孵化成鸟的心率；2）成鸟离开巢穴的次数和持续时间；3）成鸟的存在与否对鸟蛋温度的影响；4）雏鸟孵化成功率、生理参数和喙色表型。我们观察到，塑料巢的孵化温度一直较高。在温度升高时，低塑料组的缺巢次数较多，但两个塑料组的缺巢持续时间都明显低于对照组，这可能是为了减轻热应激的影响。在环境温度较高时，高塑料组的心率较高。随着环境温度的升高，低塑料组心率的增加不那么明显。对孵化成功率和幼雏的健康状况没有观察到明显的影响，但两个塑料组的血红蛋白值都很高，这可能表明雏鸡脱水。

{"title":"Plastic use as nesting material can alter incubation temperature and behaviour but does not affect yellow-legged gull chicks","authors":"Sara N. Veríssimo , Filipe Veloso , Francisco Neves , Jaime A. Ramos , Vitor H. Paiva , Ana C. Norte","doi":"10.1016/j.jtherbio.2024.104005","DOIUrl":"10.1016/j.jtherbio.2024.104005","url":null,"abstract":"<div><div>Optimal incubation temperature is crucial for embryos' development and survival. With the increasing use of plastics in gulls' nests, it is essential to understand how their incorporation affects incubation temperature, parental behaviour, and hatching success. Considering this, we conducted an experiment where plastic was introduced into yellow-legged gulls (<em>Larus michahellis</em>) nests. The experiment comprised three groups: a control group, a group with low amount of plastic, and a third with a high amount of plastic. This design allowed us to investigate the effects of plastic on 1) the heart rate of incubating adults, 2) the number and duration of adults' absences from their nest, 3) how the presence or absence of the adult influenced egg temperature, and 4) chick hatching success, physiological parameters, and bill colour phenotype. We observed that incubation temperature was consistently higher in nests with plastic. The number of absences was higher in the low plastic group at increased temperatures, though the duration was significantly lower in both plastic groups than in the control, possibly to mitigate the effects of heat stress. During higher environmental temperatures, heart rate was higher for the high plastic group. The increase in heart rate in the low plastic group was less pronounced with increasing environmental temperatures. No significant effects were observed on hatching success or in the health condition of young chicks, except for high values of haemoglobin in both plastic groups, which might indicate dehydration.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal perception and dressing behaviors of pregnant women during different stages of pregnancy: A field study at a hospital in Xi'an, China 孕妇在不同孕期的热感和穿衣行为：中国西安一家医院的实地研究

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2024-10-01 DOI: 10.1016/j.jtherbio.2024.104002

Yi Xu , Yue Li , Lijuan Wang , Ji Yang , Hui Zhang

Pregnant women have different thermal preferences during pregnancy. This paper aims to investigate the thermal characteristics of pregnant women in hospitals, addressing a research gap in the field of thermal comfort for this population. This study assessed the thermal environments of the pregnancy examination spaces (i.e., B-ultrasound room and waiting area) in a hospital located in Xi'an during the winter season. Data were collected from 1055 pregnant women at different stages of pregnancy, including demographic characteristics, thermal sensation votes (TSVs), thermal comfort votes (TCVs), and clothing insulation. The results show that the thermal sensation was significantly more influenced by the pregnancy stage than the current weight. Pregnant women in the third trimester reported a higher mean thermal sensation vote (MTSV) than in other pregnancy stages. Furthermore, the clothing insulation of pregnant women exhibited a non-normal distribution, resembling a bimodal structure. Based on this structure, typical clothing insulation values were determined to be 0.9 clo in the B-ultrasound room and 1.3 clo in the waiting area. Given these characteristics in both thermal perception and dressing behaviors of pregnant women via a subjective questionnaire, we advise lowering the temperature to ensure their comfort and save heating energy. Some personalized seats are also provided to compensate for differences in thermal preference at various stages of pregnancy. These findings contribute to the creation of comfortable and healthy pregnancy examination environments.

孕妇在怀孕期间有不同的热偏好。本文旨在研究孕妇在医院中的热特性，以解决该人群热舒适性领域的研究空白。本研究评估了西安市某医院孕期检查空间（即 B 超室和候诊区）在冬季的热环境。研究收集了 1055 名处于不同孕期的孕妇的数据，包括人口统计学特征、热感觉票数（TSV）、热舒适度票数（TCV）和衣物保温情况。结果显示，热感觉受怀孕阶段的影响明显大于当前体重的影响。怀孕三个月的孕妇的平均热感觉票数（MTSV）高于其他怀孕阶段的孕妇。此外，孕妇的衣物隔热性呈现非正态分布，类似于双峰结构。根据这一结构，B 超室的典型衣物隔热值为 0.9 clo，等候区为 1.3 clo。通过主观问卷调查，我们发现孕妇的热感知和穿衣行为都具有这些特点，因此我们建议降低温度，以确保孕妇的舒适度并节省供暖能源。此外，我们还提供了一些个性化座椅，以弥补孕妇在不同怀孕阶段的热偏好差异。这些发现有助于创造舒适健康的孕期检查环境。

{"title":"Thermal perception and dressing behaviors of pregnant women during different stages of pregnancy: A field study at a hospital in Xi'an, China","authors":"Yi Xu , Yue Li , Lijuan Wang , Ji Yang , Hui Zhang","doi":"10.1016/j.jtherbio.2024.104002","DOIUrl":"10.1016/j.jtherbio.2024.104002","url":null,"abstract":"<div><div>Pregnant women have different thermal preferences during pregnancy. This paper aims to investigate the thermal characteristics of pregnant women in hospitals, addressing a research gap in the field of thermal comfort for this population. This study assessed the thermal environments of the pregnancy examination spaces (i.e., B-ultrasound room and waiting area) in a hospital located in Xi'an during the winter season. Data were collected from 1055 pregnant women at different stages of pregnancy, including demographic characteristics, thermal sensation votes (TSVs), thermal comfort votes (TCVs), and clothing insulation. The results show that the thermal sensation was significantly more influenced by the pregnancy stage than the current weight. Pregnant women in the third trimester reported a higher mean thermal sensation vote (MTSV) than in other pregnancy stages. Furthermore, the clothing insulation of pregnant women exhibited a non-normal distribution, resembling a bimodal structure. Based on this structure, typical clothing insulation values were determined to be 0.9 clo in the B-ultrasound room and 1.3 clo in the waiting area. Given these characteristics in both thermal perception and dressing behaviors of pregnant women via a subjective questionnaire, we advise lowering the temperature to ensure their comfort and save heating energy. Some personalized seats are also provided to compensate for differences in thermal preference at various stages of pregnancy. These findings contribute to the creation of comfortable and healthy pregnancy examination environments.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0