Journal of thermal biology最新文献_第3页

Integrated transcriptomic and metabolomic analyses reveal the molecular adaptive mechanisms of Schizopygopsis chengi baoxingensis under acute heat stress 综合转录组学和代谢组学分析揭示了宝兴裂果在急性热胁迫下的分子适应机制

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2026-01-21 DOI: 10.1016/j.jtherbio.2026.104398

Hangyu Lin , Xiaoyun Wu , Ya Liu , Jun Ou , Luyun Ni , Feiyang Li , Jiansheng Lai , Quan Gong , Jue Lin , MingJiang Song

Global warming alters aquatic habitats, challenging cold-water fish survival. Schizopygopsis chengi baoxingensis, a cold-water fish from the Qingyijiang River in China, is vulnerable to temperature changes. This study aimed to elucidate the molecular mechanisms underlying the response of S. c. baoxingensis to acute heat stress through an integrated approach of histopathological, transcriptomic, and metabolomic analyses. Histopathological results revealed significant tissue damage in both the liver and gills, with the liver showing pronounced hepatocellular vacuolization and nuclear displacement, and the gills exhibiting hyperplasia and partial lamellar necrosis. Transcriptomic analysis identified numerous differentially expressed genes (DEGs) in both tissues. In the liver, DEGs were enriched in pathways related to antioxidant stress, detoxification, and immune response. Notably, genes encoding glutathione S-transferase (GST) and UDP-glucuronosyltransferase (UGT) were upregulated, suggesting enhanced antioxidant and detoxification capabilities. Additionally, immune-related genes such as C2, C3, C4, C6, C9, CFB, CFH, and MASP2 were predominantly upregulated in the complement and coagulation cascades pathway. In the gills, DEGs including NPNT and NOS2 were significantly upregulated, suggesting enhanced respiratory function. Metabolomic profiling showed significant alterations in energy metabolism, with increased levels of NAD and NADH in the liver, indicating heightened energy demands under thermal stress. Our findings provide valuable insights into the molecular mechanisms underlying the heat stress response of S. c. baoxingensis and highlight potential targets for protecting this species under climate change.

全球变暖改变了水生生物的栖息地，挑战了冷水鱼类的生存。青衣江裂腹鱼是一种来自中国青衣江的冷水鱼，它很容易受到温度变化的影响。本研究旨在通过组织病理学、转录组学和代谢组学的综合分析，阐明宝兴s.c.对急性热应激反应的分子机制。组织病理学结果显示肝脏和鳃均有明显的组织损伤，肝脏表现出明显的肝细胞空泡化和核移位，而鳃表现出增生和部分板层坏死。转录组学分析在两种组织中发现了许多差异表达基因（DEGs）。在肝脏中，DEGs在与抗氧化应激、解毒和免疫反应相关的途径中富集。值得注意的是，编码谷胱甘肽s -转移酶（GST）和udp -葡萄糖醛基转移酶（UGT）的基因上调，表明抗氧化和解毒能力增强。此外，免疫相关基因如C2、C3、C4、C6、C9、CFB、CFH和MASP2在补体和凝血级联通路中主要上调。在鳃中，包括NPNT和NOS2在内的DEGs显著上调，表明呼吸功能增强。代谢组学分析显示能量代谢发生了显著变化，肝脏中NAD和NADH水平升高，表明热应激下能量需求增加。我们的研究结果为揭示保兴杉树热应激反应的分子机制提供了有价值的见解，并为该物种在气候变化下的保护提供了潜在的目标。

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引用次数: 0

Pathogens may have assisted the evolution of endothermy by restricting its reversibility 病原体可能通过限制恒温动物的可逆性来促进其进化

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2026-01-20 DOI: 10.1016/j.jtherbio.2026.104385

Shahar Dubiner

Endothermy is an important trait in the biology of several (extant and extinct) groups of amniotes. Despite its distinct benefits, it is an extremely expensive trait, and the drivers for its emergence and evolutionary success are debated. I offer a hypothesis complementary to current theories, linking endotherms’ capacity for thermogenesis with the need to increase body temperature during pathogen infection (“fever”, present in both endotherms and ectotherms). I do not propose this as a primary driver of endothermy; rather, the emergence of efficient fever prevented the secondary loss of endothermy. After endothermy has evolved in a given lineage, the stabler host temperature, coupled with higher direct transmission due to incubation and parental care, would lead to the propagation and specialization of pathogens in the population. Hence, although ectothermy carries no inherent disadvantage, reverting from endothermy to ectothermy faces the obstacle of an already-high pathogen load. Reduced heat production would increase the gap from normal to fever temperature, impairing the reliability of the response and increasing its cost, when pathogen load in the population is already higher and more specialized as enabled by endothermy. This factor may be enough to outweigh selective pressures against the energetic cost of endothermy. This hypothesis, though anecdotally supported by the intriguing fact that endothermy is very rarely lost (and is often retained even when homeothermy is not), is merely a conceptual framework and must be tested further.

恒温是几个（现存的和灭绝的）羊膜动物群的一个重要的生物学特征。尽管它有明显的好处，但它是一种极其昂贵的特征，其出现和进化成功的驱动因素一直存在争议。我提出了一个补充现有理论的假设，将恒温动物的产热能力与病原体感染（“发烧”，在恒温动物和变温动物中都存在）期间体温升高的需要联系起来。我不认为这是恒温动物的主要驱动因素；相反，有效发热的出现阻止了吸热动物的继发性丧失。在一个特定的谱系中，恒温动物进化后，稳定的宿主温度，加上由于孵化和亲代照顾而产生的更高的直接传播，将导致病原体在群体中的繁殖和专业化。因此，尽管变温动物没有固有的劣势，但从恒温动物向变温动物的转变面临着已经很高的病原体负荷的障碍。减少产热会增加从正常温度到发烧温度之间的差距，损害反应的可靠性并增加其成本，当种群中的病原体负荷已经很高并且由于恒温而更加专业化时。这一因素可能足以抵消对抗恒温动物能量消耗的选择压力。这一假设，虽然有一个有趣的事实支持，即恒温动物很少消失（即使在恒温动物没有消失的情况下，也经常被保留），但仅仅是一个概念框架，必须进一步验证。

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引用次数: 0

Interactions between brown adipose tissue activity and exercise modality on metabolic kinetics: a crossover study in trained individuals 代谢动力学中棕色脂肪组织活动和运动方式之间的相互作用：一项训练个体的交叉研究。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2026-01-18 DOI: 10.1016/j.jtherbio.2026.104392

Dany Alexis Sobarzo Soto , Diego Ignácio Valenzuela Pérez , Naiara Ribeiro Almeida , Bianca Miarka , Armin Isael Alvarado Oyarzo , João Carlos Bouzas Marins , Manuel Sillero-Quintana , Andreia Cristiane Carrenho Queiroz , Ciro José Brito

This study aimed to investigate the interactions between basal brown adipose tissue (BAT) activity and exercise modality on energy expenditure and substrate oxidation kinetics in trained individuals. Thirty-five trained males were stratified into high (HBAT, n = 15: 26.5 ± 4.3 years, 25.5 ± 1.8 kg/m²) and low (LBAT, n = 20: 27.0 ± 4.1 years, 26.0 ± 1.7 kg/m²) BAT activity groups based on infrared thermography evaluation. In a crossover design, each participant performed two experimental sessions: continuous aerobic exercise (AERO: 30 min at 70–80 % HR_reserve) and high-intensity interval exercise (HIIE: 4 × 4 min at 90–95 % HRmax with 3-min active recovery), separated by 7 days. energy expenditure and substrate oxidation including carbohydrate, lipid and protein were continuously measured by indirect calorimetry during exercise and throughout a 30-min recovery period. Polynomial regression modeling of temporal trajectories revealed a significantly higher total energy expenditure in the HBAT group compared to the LBAT group (+25 %, p < 0.01), with the peak energy expenditure rate observed during HIIE in the HBAT group (3.7 ± 0.2 kcal min⁻¹ versus 2.9 ± 0.3 kcal min⁻¹ in AERO-LBAT; p = 0.004). Carbohydrate oxidation displayed a characteristic biphasic decline but showed no significant differences between groups. In contrast, lipid oxidation peaked later and was substantially higher in the HBAT groups, with the most pronounced effect following HIIE. Protein oxidation remained minimal and consistent across all conditions. These findings demonstrate that elevated BAT activity is associated with increased exercise-induced energy expenditure and enhanced lipid utilization during the latter stages of exercise, with HIIE showing the most robust metabolic associations in individuals with high BAT activity.

本研究旨在探讨基础棕色脂肪组织（BAT）活性和运动方式对训练个体能量消耗和底物氧化动力学的相互作用。根据红外热成像评价，将35名训练男性分为BAT活性高组（HBAT, n = 15: 26.5±4.3年，25.5±1.8 kg/m2）和BAT活性低组（LBAT, n = 20: 27.0±4.1年，26.0±1.7 kg/m2）。在交叉设计中，每个参与者进行两个实验：连续有氧运动（AERO: 30分钟，70- 80% HRmax）和高强度间歇运动（HIIE: 4 × 4分钟，90- 95% HRmax， 3分钟主动恢复），间隔7天。在运动期间和整个30分钟的恢复期，通过间接量热法连续测量能量消耗和底物氧化，包括碳水化合物、脂质和蛋白质。时间轨迹的多项式回归模型显示，与LBAT组相比，HBAT组的总能量消耗显著更高（+ 25%,p -1，而AERO-LBAT组为2.9±0.3 kcal min-1, p = 0.004）。碳水化合物氧化表现出典型的双相下降，但组间差异不显著。相比之下，脂质氧化在HBAT组中达到峰值较晚，并且在HIIE组中明显更高，效果最明显。在所有条件下，蛋白质氧化保持最小且一致。这些发现表明，在运动后期，BAT活性升高与运动引起的能量消耗增加和脂质利用增强有关，HIIE在BAT活性高的个体中显示出最强大的代谢关联。

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引用次数: 0

Circadian modulation of core temperature and thermoregulatory strain during live-fire compartment exposure in firefighters 消防员实弹室暴露期间核心温度和体温调节应变的昼夜节律调节

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2026-01-17 DOI: 10.1016/j.jtherbio.2026.104383

Benoit Mauvieux , Adrian Markov , Stéphane Besnard , Yvan Touitou , Ben J. Edwards

Firefighters are repeatedly exposed to extreme radiant and convective heat during live-fire training, yet the potential influence of circadian timing on their thermoregulatory tolerance remains unexplored. This study tested whether time-of-day modulates physiological strain during standardized container fire exposures. Twenty-one professional male firefighters completed two identical 40-min live-fire sessions on the same day: late-morning (09:00 h, heat-gain phase) and late-evening (21:30 h, heat-loss phase). Core temperature (ingestible sensor), heart rate, skin temperature, under-PPE temperature and humidity, body mass, total body water, and ratings of perceived exertion were recorded. Environmental conditions were strictly matched between sessions. Core temperature rose faster and higher in the morning (ΔTcore +1.10 ± 0.25 °C; slope 0.028 °C·min⁻¹) than in the evening (+0.49 ± 0.21 °C; 0.012 °C·min⁻¹), despite similar peak values. Post-exposure cooling was slower in the morning (−0.37 vs −0.63 °C·h⁻¹), with a delayed hypothermic rebound. Morning sessions also elicited higher heart rates, greater perceived exertion, larger body-mass and water losses, and higher sub-garment humidity. These findings demonstrate that circadian phase significantly influences heat storage and recovery, with late-morning exposures imposing greater thermophysiological strain under identical workloads. Incorporating chronobiological principles into firefighter training schedules may reduce heat-related risk and optimize recovery strategies in extreme environments.

消防员在实弹训练中反复暴露在极端的辐射和对流热下，然而昼夜节律对其热调节耐受性的潜在影响仍未被探索。本研究测试了在标准化容器火灾暴露期间，时间是否会调节生理应变。21名职业男性消防员在同一天完成了两次相同的40分钟实弹射击：上午晚些时候（09:00小时，热量增加阶段）和晚上晚些时候（21:30小时，热量损失阶段）。记录核心温度（可摄取传感器）、心率、皮肤温度、ppe下的温度和湿度、体重、全身水分和感知运动的评分。两组之间的环境条件严格匹配。上午（ΔTcore +1.10±0.25°C；斜率0.028°C·min - 1）的核心温度上升速度较快，高于晚上（+0.49±0.21°C; 0.012°C·min - 1），但峰值相似。暴露后的降温在早晨较慢（- 0.37 vs - 0.63°C·h - 1），伴有延迟的低温反弹。早晨锻炼还会引起更高的心率、更大的运动感、更大的体重和水分流失，以及更高的衣服亚湿度。这些研究结果表明，昼夜节律显著影响热量储存和恢复，在相同的工作量下，上午晚些时候的暴露会施加更大的热生理压力。将时间生物学原理纳入消防员训练计划可以减少与热有关的风险，并优化极端环境下的恢复策略。

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引用次数: 0

Living in the city has its advantages: thermoregulation behavior and activity time in the Torquate lizard 生活在城市里有它的优势：Torquate蜥蜴的体温调节行为和活动时间

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2026-01-12 DOI: 10.1016/j.jtherbio.2026.104380

Juan C. González-Morales , Gabriel Suárez-Varón , Elizabeth Bastiaans , Gregorio Moreno-Rueda , Heliot Zarza

Urban development transforms natural ecosystems, imposing novel challenges on the organisms that persist within them. One such change is the "heat island" effect, which involves higher temperatures in urban areas compared to non-urban areas. Therefore, identifying the traits related to persisting in or colonizing urban environments is crucial for developing conservation plans for urban vertebrates, especially reptiles, which are highly dependent on ambient temperature. This study examines thermal traits and potential activity time in Sceloporus torquatus lizards inhabiting an urban and a non-urban site in central Mexico. We assessed body temperatures in the field, thermal preferences in a laboratory gradient, and critical thermal limits. We used biophysical modeling to simulate annual activity time under two vegetation cover scenarios corresponding to these urban and non-urban environments. Despite finding similar body and preferred temperatures in the two populations, lizards from the urban site were larger, showed lower critical thermal values, and were more effective in thermoregulation, as measured by their lower deviation from preferred temperature. Lizards from the urban site also likely had more hours of activity across the year, likely due to warmer conditions associated with the urban heat island effect. While these traits may offer short-term advantages, continued increases in temperature could reduce daily activity windows and challenge persistence in this urban site. Our findings underscore the importance of considering both physiological thresholds and habitat features, such as vegetation cover and microhabitat structure, when assessing how ectothermic species respond to urban pressures.

城市发展改变了自然生态系统，给生存在其中的生物带来了新的挑战。其中一个变化是“热岛”效应，即城市地区的温度高于非城市地区。因此，确定与在城市环境中生存或定居相关的特征，对于制定城市脊椎动物，特别是高度依赖环境温度的爬行动物的保护计划至关重要。本研究考察了居住在墨西哥中部城市和非城市地区的torquatus蜥蜴的热特性和潜在活动时间。我们评估了野外的体温、实验室梯度下的热偏好和临界热极限。利用生物物理模型模拟了城市和非城市两种植被覆盖情景下的年活动时间。尽管在两个种群中发现了相似的身体和偏好温度，但城市地区的蜥蜴体型更大，表现出更低的临界热值，并且在温度调节方面更有效，因为它们与偏好温度的偏差更小。城市地区的蜥蜴全年的活动时间也可能更长，这可能是由于城市热岛效应带来的温暖环境。虽然这些特征可能提供短期优势，但持续升高的温度可能会减少日常活动窗口，并挑战该城市站点的持久性。我们的研究结果强调了在评估变温物种如何应对城市压力时，考虑生理阈值和栖息地特征（如植被覆盖和微栖息地结构）的重要性。

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引用次数: 0

Genetic component of sensitivity to heat stress for fertility traits of Jersey crossbred cattle 泽西杂交牛育性性状对热胁迫敏感性的遗传成分

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2026-01-11 DOI: 10.1016/j.jtherbio.2026.104376

Indrajit Gayari , Sylvia Lalhmingmawii , Lalmalsawmi Colney , Hasan Baneh , Ajoy Mandal

Heat stress (HS) attributed by climate change, is a significant challenge to livestock production globally, particularly affecting dairy cows' fertility. This not only leads to decreased reproductive performance but threatens the herd profitability. The aim of this study was to analyze the effect of heat stress on non-return rate (NR56) and conception rate (CR) in Jersey × Bos indicus crossbred cows, to identify the threshold at which these traits are affected, and to estimate genetic parameters and variance components under HS conditions. The study analyzed insemination records of 1174 crossbreds over a period of 26 years, with the use of temperature-humidity index (THI) as an indicator to measure HS. Logistic regression models and Bayesian statistics were employed to identify THI breakpoints (BP) for NR56 and CR. Genetic parameters were estimated using reaction norm models and Gibbs sampling approach for evaluation of variance components. THI breakpoints were identified at 77 for NR56 and 68 for CR. The heritability of NR56 decreased as THI increased, while CR heritability exhibited a peak at higher THI levels. Genetic correlations indicated antagonistic relationships between general additive and HS-specific additive effects for both traits, with increasing additive and environmental variance as THI rose. Thus, this study highlights the genetic diversity associated with heat tolerance in crossbred cows and suggests that selecting for genetically heat-tolerant animals may mitigate HS related fertility declines. Further, these findings provide insight into breeding strategies that can be implemented to enhance reproductive performance of crossbred dairy cow in tropical climatic conditions.

由气候变化引起的热应激（HS）是全球畜牧业生产面临的重大挑战，特别是影响奶牛的生育力。这不仅导致繁殖性能下降，而且威胁到畜群的盈利能力。本研究旨在分析热应激对泽牛不回报率（NR56）和受胎率（CR）的影响，确定热应激条件下这些性状的影响阈值，并估计热应激条件下泽牛不回报率和受胎率的遗传参数和方差成分。本研究分析了1174个杂交品种26年的授精记录，采用温湿度指数（THI）作为衡量HS的指标。采用Logistic回归模型和贝叶斯统计方法确定NR56和CR的THI断点（BP），采用反应范数模型估计遗传参数，采用Gibbs抽样方法评估方差成分。NR56的THI断点为77，CR的THI断点为68，NR56的遗传力随着THI的增加而降低，而CR的遗传力在THI水平较高时达到峰值。遗传相关表明，这两个性状的一般加性效应和hs特异性加性效应之间存在拮抗关系，随着THI的升高，加性效应和环境方差均增加。因此，本研究强调了与杂交奶牛耐热性相关的遗传多样性，并表明选择基因耐热的动物可能会减轻HS相关的生育力下降。此外，这些发现为在热带气候条件下提高杂交奶牛繁殖性能的育种策略提供了见解。

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引用次数: 0

Environmental, physiological, perceptual, and behavioral predictors of mean and final km paces during a laboratory-based self-paced 10 km run in hot conditions 环境、生理、知觉和行为预测在炎热条件下基于实验室的自定速10公里跑的平均和最终公里配速。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2026-01-10 DOI: 10.1016/j.jtherbio.2026.104374

Marcelo T. Andrade , Matheus M.S. Nunes-Leite , Rúbio S. Bruzzi , Carlos H. Souza , João P. Uendeles-Pinto , Nathan B. Amarante de Sá , Thiago T. Mendes , Luciano S. Prado , Cândido C. Coimbra , Danusa D. Soares , Samuel P. Wanner

Endurance performance is regulated by integrated physiological, perceptual, and behavioral mechanisms, and is markedly impaired by environmental heat stress. However, how heat stress influences this multifaceted regulation of performance merits further investigation. This study aimed to identify predictors of mean and final km paces during a laboratory-based run in hot conditions. We analyzed 75 records from recreational athletes who ran 10 km as fast as possible at 33 °C. The independent variables included maximal oxygen uptake (VO_2MAX), whole-body sweat loss, fan-generated airflow, wet-bulb globe temperature (WBGT), change in core temperature (ΔT_CORE: entire exercise and its first five km), heart rate (HR), rating of perceived exertion (RPE), pacing strategy, biological sex, thermal comfort, the core-to-skin temperature difference, and percentage change in body mass (Δ% body mass). These independent variables were selected a priori based on theoretical models that explain pacing regulation in an integrative manner. The dependent variables were the mean and final km paces (min/km), and predictors were analyzed using hierarchical multiple linear regression. Mean pace was best predicted by air velocity (β = −0.494), ΔT_CORE (β = −0.456), VO_2MAX (β = −0.387), WBGT (β = 0.294), ΔT_CORE 0–5 km (β = 0.323), and Δ% body mass (β = −0.191), with the model explaining 54.3 % of the dependent variable's variance. Final km pace was best predicted by the adoption of a negative-split pacing strategy (β = 0.387), followed by HR (β = −0.206) and RPE (β = −0.188), with the model explaining 30.3 % of the dependent variable's variance. The current findings reinforce the notion that the regulation of mean and final km paces integrates physiological, environmental, behavioral, and perceptual mechanisms. Our findings also indicate that interventions to improve athletes' performance in hot conditions should combine aerobic fitness development, early T_CORE control, optimized airflow and hydration, and RPE-guided conservative/negative-split pacing.

耐力表现受生理、知觉和行为机制的综合调节，并明显受到环境热应激的损害。然而，热应激如何影响这种多方面的性能调节值得进一步研究。本研究旨在确定在炎热条件下实验室运行期间平均和最终公里速度的预测因子。我们分析了75名业余运动员的记录，他们在33°C的条件下尽可能快地跑10公里。自变量包括最大摄氧量（VO2MAX）、全身失汗量、风扇产生的气流、湿球温度（WBGT）、核心温度变化（ΔTCORE：整个运动及其前5公里）、心率（HR）、感知运动评分（RPE）、起搏策略、生理性别、热舒适度、核心-皮肤温差和体重变化百分比（Δ%体重）。这些自变量是基于综合解释心律调节的理论模型先验选择的。因变量为平均和最终公里间距（min/km），预测因子采用层次多元线性回归分析。风速（β = -0.494）、ΔTCORE （β = -0.456）、VO2MAX （β = -0.387）、WBGT （β = 0.294）、ΔTCORE 0-5 km （β = 0.323）和Δ%体重（β = -0.191）最能预测平均步速，该模型解释了54.3%的因变量方差。采用负分割起搏策略对最终公里配速的预测效果最好（β = 0.387），其次是HR （β = -0.206）和RPE (β = -0.188)，该模型解释了30.3%的因变量方差。目前的研究结果强化了这样一种观点，即平均和最终公里速度的调节综合了生理、环境、行为和感知机制。我们的研究结果还表明，提高运动员在高温条件下的表现的干预措施应该结合有氧健身发展，早期TCORE控制，优化气流和水合作用，以及rpe引导的保守/负分裂起搏。

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引用次数: 0

Exploring the protective mechanism of exogenous melatonin on intestine of heat-stressed chicks based on network pharmacology and intestinal microbiota analysis 基于网络药理学和肠道菌群分析探讨外源性褪黑素对热应激雏鸡肠道的保护机制。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2026-01-10 DOI: 10.1016/j.jtherbio.2026.104371

Xiang-Yang Xu , Xin Kang , Meng-Meng Liu , Shu-Qi Zhang , Sai-Nan Dong , Xiao-Wen Jiang , Wen-Hui Yu

Heat stress constitutes a severe risk to the health of poultry, not only damaging intestinal health, but also reducing antioxidant defenses and weakening the immune response. Exogenous melatonin can enhance the antioxidant capacity of heat-stressed chicks through the cecal microbiota, and reduce the inflammatory response and intestinal barrier damage. 21-day-old Hy-Line brown chicks were divided into control group, heat stress group, and three groups receiving different doses of melatonin (0.5, 1.0, 2.0 mg/kg). Except for the control group (22 ± 2 °C from 00:00 to 24:00 daily), all other groups were exposed to a high-temperature environment (34 ± 2 °C, from 08:00 to 18:00 daily) for 21 consecutive days. The results showed that heat stress significantly decreased final body weight of chicks, increased serum MDA, IL-1β, IL-6, TNF-α, D-LA and DAO levels, decreased GSH-Px, CAT and SOD activities, and decreased jejunal and ileal villus height/crypt depth (VH/CD), cecal Firmicutes/Bacteroidetes (F/B) ratio and Lactobacillus abundance. Exogenous melatonin reversed these changes, and 1.0 mg/kg was the most effective. It included the recovery of antioxidant enzyme activity, the decrease of serum inflammatory factors, D-LA and DAO, the up-regulation of VH/CD and tight junction genes (CLDN1, OCLN, TJP1, MUC2) in jejunum and ileum, the increase of cecal F/B ratio and the enrichment of Lactobacillus. Network pharmacology and molecular docking identified the core targets of anti-oxidation and anti-inflammation. Melatonin reduced heat stress-induced intestinal injury by reducing inflammation and oxidative stress.

热应激对家禽健康构成严重威胁，不仅会损害肠道健康，还会降低抗氧化防御能力，削弱免疫反应。外源性褪黑素可以通过盲肠菌群增强热应激雏鸡的抗氧化能力，减少炎症反应和肠道屏障损伤。21日龄海兰褐鸡分为对照组、热应激组和3组，分别饲喂0.5、1.0、2.0 mg/kg褪黑素。除对照组（22±2°C，每天00:00 - 24:00）外，其余各组连续21天暴露于高温环境（34±2°C，每天08:00 - 18:00）。结果表明，热应激显著降低了雏鸡的最终体重，提高了血清MDA、IL-1β、IL-6、TNF-α、D-LA和DAO水平，降低了GSH-Px、CAT和SOD活性，降低了空肠和回肠绒毛高度/隐窝深度（VH/CD）、盲肠厚壁菌门/拟杆菌门（F/B）比和乳酸菌数量。外源性褪黑素逆转了这些变化，其中1.0 mg/kg最有效。包括抗氧化酶活性恢复，血清炎症因子、D-LA和DAO降低，空肠和回肠VH/CD和紧密连接基因（CLDN1、OCLN、TJP1、MUC2）上调，盲肠F/B比升高，乳酸菌富集。网络药理学与分子对接，确定了抗氧化、抗炎的核心靶点。褪黑素通过减少炎症和氧化应激来减少热应激引起的肠道损伤。

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引用次数: 0

Corrigendum to "Treatment of accidental hypothermia: Impact of insulation placement above or below an active external rewarming device on temperature and burn risk" [J. Therm. Biol. (129), 2025, 104126]. “意外体温过低的治疗：主动外部复温装置上方或下方的绝缘放置对温度和烧伤风险的影响”的更正[J]。小卡。医学杂志。[j].地球科学进展，2002,26(4):426 - 426。

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2026-01-10 DOI: 10.1016/j.jtherbio.2026.104368

Sigurd Mydske, Guttorm Brattebø, Ane Marthe Helland, Øystein Wiggen, Jörg Assmus, Øyvind Thomassen

引用次数: 0

Elevation shapes thermal breadth and climate sensitivity in Moltrecht's treefrog tadpoles 海拔塑造了Moltrecht树蛙蝌蚪的热宽度和气候敏感性

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology

Pub Date : 2026-01-10 DOI: 10.1016/j.jtherbio.2026.104369

Jh-Yu You , Pol Pintanel , Ming-Feng Chuang

Global climate change causes rising annual temperatures and an increase in extreme climate events, posing higher risks to organisms. Understanding the thermal tolerance of organisms is therefore crucial to evaluate the temperature limits they can withstand. Previous studies have hypothesized that species thriving in regions with higher climatic variability tend to have broader thermal tolerance breadth to adapt to such environments, a concept known as the climate variability hypothesis. However, few studies have explored whether different populations of the same species, distributed across varying environments, follow this trend. In this study, we examined the critical thermal tolerance abilities and recorded the microclimates of Moltrecht's green treefrog (Zhangixalus moltrechti) tadpoles across different elevations. We aimed to test the climate variability hypothesis and assess the plasticity of their upper and lower thermal tolerance limits. Our results revealed that tadpoles from higher elevations exhibit a broader thermal tolerance breadth, with elevation primarily influencing their critical thermal minimum. Additionally, the plasticity of cold tolerance was greater than that of heat tolerance. Our study highlights that even within the same species, populations in different environments face distinct threats from climate change. Notably, global warming poses a greater risk to low-elevation populations.

全球气候变化导致年气温上升和极端气候事件增加，给生物带来更高的风险。因此，了解生物体的耐热性对于评估它们所能承受的温度限制至关重要。以前的研究假设，在气候变异性较高的地区，物种往往具有更广泛的热耐受广度来适应这种环境，这一概念被称为气候变异性假说。然而，很少有研究探讨分布在不同环境中的同一物种的不同种群是否遵循这一趋势。研究了不同海拔地区绿树蛙（Zhangixalus moltrechti）蝌蚪的临界耐热能力，并记录了它们的小气候特征。我们的目的是验证气候变率假说，并评估其上下限热容极限的可塑性。研究结果表明，高海拔地区的蝌蚪表现出更宽的热容忍宽度，海拔主要影响其临界热最小值。耐寒性的可塑性大于耐热性。我们的研究强调，即使在同一物种中，不同环境中的种群也面临着气候变化带来的不同威胁。值得注意的是，全球变暖对低海拔地区的人口构成了更大的风险。

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