Journal of Traditional and Complementary Medicine最新文献_第8页

Antispasmodic, cardioprotective and blood-pressure lowering properties of Gomphrena perennis L. and its mechanisms of action Gomphrena perennis L.的解痉、心脏保护和降血压特性及其作用机制

IF 4.5 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2023-10-31 DOI: 10.1016/j.jtcme.2023.10.005

Adriana Milena Bonilla Bonilla , Tatiana C. Gavilánez Buñay , Matías Bayley , Germán A. Colareda , Soledad I. Matera , María Luján Flores , Osvaldo León Córdoba , Julián J. Prieto , María Esperanza Ruiz , Alicia E. Consolini , María Inés Ragone

Background

Gomphrena perennis L. is a native plant of South America whose pharmacological properties have not been studied yet.

Aim

To evaluate the cardiovascular and intestinal pharmacological effects of Gomphrena perennis L. leaves tincture (GphT) and the mechanisms involved.

Experimental procedure

The chromatographic profile of GphT was done. Its ex vivo effects were evaluated by contractile concentration-response curves (CRCs) obtained from the agonist carbachol or calcium found in isolated rat small intestine, as well as in the relaxant CRCs. Cardiac effects were evaluated on isolated rat hearts exposed to ischemia/reperfusion (I/R). Experiments in vivo were performed to evaluate the diuretic activity in conscious rats and the hypotensive effect in anaesthetised rats.

Results

Fifteen flavonoids were identified in GphT by HPLC-UV, including diosmin. GphT induced a non-competitive inhibition in both carbachol and calcium CRCs on rat small intestine. The first was not affected by indomethacin. Moreover, GphT, unlike diosmin, relaxed the contracture produced by a high-potassium solution in a dose-dependently way. Neither propranolol nor l-NAME changed it. GphT did not show diuretic activity but induced hypotension insensitive to l-NAME. While GphT perfusion of isolated hearts increased injury consequent to I/R, oral administration was cardioprotective and reversed by l-NAME. However, diosmin did not improve the post-ischemic recovery.

Conclusions

This study supports the use of Gomphrena perennis L. tincture as an antispasmodic and hypotensive agent. Moreover, it has been demonstrated to be preventive of post-ischemic cardiac dysfunction. However, diosmin would not be responsible for these effects.

背景Gomphrena perennis L.是南美洲的一种原生植物，其药理特性尚未得到研究。目的评估Gomphrena perennis L.叶酊（GphT）对心血管和肠道的药理作用及其机制。通过在离体大鼠小肠中发现的激动剂卡巴胆碱或钙获得的收缩浓度-反应曲线（CRCs）以及松弛剂 CRCs 对其体内效应进行了评估。对暴露于缺血/再灌注（I/R）的离体大鼠心脏的心脏效应进行了评估。通过高效液相色谱-紫外检测，在 GphT 中发现了 15 种黄酮类化合物，其中包括地奥司明。GphT 对大鼠小肠上的卡巴胆碱和钙 CRC 均有非竞争性抑制作用。前者不受吲哚美辛的影响。此外，与地奥司明不同，GphT 能以剂量依赖的方式放松高钾溶液产生的收缩。普萘洛尔和l-NAME都不能改变它。GphT没有利尿活性，但能诱导对l-NAME不敏感的低血压。虽然GphT灌注离体心脏会加重I/R造成的损伤，但口服GphT对心脏有保护作用，并能被l-NAME逆转。结论本研究支持将 Gomphrena perennis L. 酊剂用作解痉和降压药。此外，它还被证明可预防缺血后心功能不全。然而，这些作用并不是由地奥司明引起的。

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引用次数: 0

Qingguang'an-induced autophagy in TFs inhibits scar formation: A follow-up in vivo mechanistic investigation 青光庵诱导的 TFs 自噬可抑制瘢痕形成：体内机制的后续研究

IF 4.5 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2023-10-18 DOI: 10.1016/j.jtcme.2023.10.002

Rong Hu , Xian-jing Wang , Shu Chen , Yun Huang , Juan Yu

Purpose

To investigate the mechanism by which Qingguang'an inhibits scar formation in rabbits administered glaucoma filtering surgery (GFS).

Methods

Combined trabeculectomy was performed in 100 rabbits diagnosed with glaucoma, which were assigned to five groups, including the no surgery, surgery only, mitomycin C (MMC; positive control), Qingguang'an (experimental) and PBS (negative control) groups. The animals were followed up at postoperative days 1–28. Ultrastructure was observed under a transmission electron microscope (TEM). Real-Time Polymerase Chain Reaction (RT-PCR), Western blot, Hematoxylin and Eosin (H&E) staining, Masson's trichrome staining and Immuno-histochemistry (IHC) were performed to assess the harvested blocks.

Results

In the Qingguang'an group, intraocular pressure (IOP) on postoperative D28 was significantly lower than values in the no surgery, surgery only and PBS groups (P < 0.05). Its blebs kept better filtering function and less complications in follow-up, which be detected to have less fibroblasts and collagen deposition histologically. Compared with the PBS group, ATG5, Beclin1 and LC3-II mRNA levels were significantly increased while P62 was downregulated in the Qingguang'an group (P < 0.05). Correspondingly, ATG5 and Beclin1 protein amounts in the Qingguang'an group were increased while P62 was downregulated. The LC3-II/Ⅰ ratio tended to rise to the process of autophagy. Abundant autophagosomes were captured under TEM in this condition.

Conclusions

Qingguang'an granules can inhibit scar formation in rabbits after GFS and restrain IOP increase by inducing autophagy in TFs.

目的研究青光眼滤过手术（GFS）对兔子瘢痕形成的抑制机制。方法对100只确诊为青光眼的兔子进行联合小梁切除术，将其分为五组，包括不手术组、单纯手术组、丝裂霉素C组（MMC；阳性对照组）、青光眼滤过手术组（实验组）和PBS组（阴性对照组）。术后第 1-28 天对动物进行随访。在透射电子显微镜（TEM）下观察超微结构。结果在青光眼组，术后第 28 天的眼压（IOP）明显低于未手术组、单纯手术组和 PBS 组（P < 0.05）。其出血点在随访中保持了较好的滤过功能，并发症较少，在组织学上可检测到较少的成纤维细胞和胶原沉积。与 PBS 组相比，青光眼组的 ATG5、Beclin1 和 LC3-II mRNA 水平明显升高，而 P62 水平下调（P < 0.05）。相应地，青光庵组 ATG5 和 Beclin1 蛋白含量增加，而 P62 蛋白含量下降。LC3-Ⅱ/Ⅰ比值呈上升趋势，这与自噬过程有关。结论青光庵颗粒通过诱导TFs自噬，可抑制家兔GFS后瘢痕形成，抑制眼压升高。

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引用次数: 0

Corrigendum to ‘Wound healing efficacy of Jamun honey in diabetic mice model through reepithelialization, collagen deposition and angiogenesis’ [J. Trad, Complementary Med 10 (6) (November 2020) 529–543] 通过再上皮化、胶原沉积和血管生成，Jamun 蜂蜜对糖尿病小鼠模型的伤口愈合功效"[J. Trad, Complementary Med 10 (6) (November 2020) 529-543] 更正

IF 4.5 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2023-10-04 DOI: 10.1016/j.jtcme.2023.10.001

Amrita Chaudhary , Swarnendu Bag , Provas Banerjee , Jyotirmoy Chatterjee

引用次数: 0

Dachengqi decoction ameliorates sepsis-induced liver injury by inhibiting the TGF-β1/Smad3 pathways 大承气汤通过抑制 TGF-β1/Smad3 通路改善败血症引起的肝损伤

IF 4.5 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2023-09-13 DOI: 10.1016/j.jtcme.2023.09.001

Guangtao Pan , Yanran Wu , Yuhan Liu , Fangyuan Zhou , Sen Li , Shenglan Yang

Background

Sepsis-induced acute liver injury (ALI) is a major contributor to mortality in septic patients. Exploring the pathogenesis and developing effective treatment strategies for sepsis-induced ALI is critical for improving patient outcomes. Dachengqi decoction (DCQD), which is a classic Chinese herbal medicine, has been shown to possess potent anti-inflammatory properties. However, the protective effects and underlying mechanisms of DCQD against sepsis-induced ALI remain unclear. This study aimed to investigate the protective effect of DCQD on sepsis-induced ALI and elucidate the involvement of the TGF-1β/Smad3 pathways.

Methods

A septic mouse model was established using caecal ligation and puncture (CLP) to evaluate the protective effect of DCQD on sepsis-induced ALI in vivo. An in vitro cellular inflammation model was established using LPS-stimulated LO2 cells to further investigate the underlying mechanism.

Results

DCQD (2.5, 5.0, and 10.0 g/kg body weight) was administered twice daily for 2 days and exerted a dose-dependent protective effect against sepsis-induced ALI. DCQD treatment significantly inhibited inappropriate inflammatory responses and oxidative stress in liver tissue. Moreover, DCQD maintained liver homeostasis by inhibiting hepatocyte apoptosis and improving sepsis-induced liver damage. In vivo and in vitro studies indicated that the TGF-β1/Smad3 signalling pathway played an important role in sepsis-induced ALI, and DCQD treatment significantly inhibited the activation of this pathway.

Conclusions

DCQD can effectively suppress excessive inflammatory responses and oxidative stress, leading to a substantial reduction in hepatocyte apoptosis in sepsis-induced ALI.

背景脓毒症诱发的急性肝损伤（ALI）是导致脓毒症患者死亡的主要原因。探索脓毒症诱发急性肝损伤的发病机制并制定有效的治疗策略对于改善患者预后至关重要。大承气汤（DCQD）是一种经典中药，已被证明具有强大的抗炎特性。然而，大承气汤对脓毒症诱发的 ALI 的保护作用及其机制仍不清楚。本研究旨在探讨DCQD对脓毒症诱发的ALI的保护作用，并阐明TGF-1β/Smad3通路的参与作用。方法利用盲肠结扎术（CLP）建立脓毒症小鼠模型，评估DCQD对脓毒症诱发的ALI的体内保护作用。结果DCQD（2.5、5.0和10.0克/千克体重）每天给药两次，持续2天，对脓毒症诱发的ALI具有剂量依赖性保护作用。DCQD能明显抑制肝组织中不适当的炎症反应和氧化应激。此外，DCQD 还能抑制肝细胞凋亡，改善败血症引起的肝损伤，从而维持肝脏的稳态。体内和体外研究表明，TGF-β1/Smad3 信号通路在脓毒症诱发的 ALI 中发挥了重要作用，而 DCQD 治疗可显著抑制该通路的激活。

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引用次数: 0

Wound healing potential of Acacia catechu in streptozotocin-induced diabetic mice using in vivo and in silico approach 儿茶对链脲佐菌素诱导的糖尿病小鼠伤口愈合潜力的体内和体外研究

IF 4.5 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2023-09-01 DOI: 10.1016/j.jtcme.2023.05.001

Vinayak P. Nakhate , Natasha S. Akojwar , Saurabh K. Sinha , Amarsinh D. Lomte , Mahaveer Dhobi , Prakash R. Itankar , Satyendra K. Prasad

Background and aim

Acacia catechu Wild. (Fabaceae) barks are traditionally used in the treatment of diabetes and wounds. Therefore, the objective of the present study was to evaluate the wound healing potential of the alcoholic extract of A. catechu (EAC) in streptozotocin-induced diabetic mice.

Experimental procedures

EAC was first subjected to phytochemical estimations and standardization using (−) epicatechin as marker with the help of HPLC. Diabetes was induced in mice using streptozotocin and the wound healing potential of EAC was evaluated using excision and incision wound models on topical and oral treatment. Various biochemical parameters, in vivo antioxidants, cytokine profiling, VEGF, and histopathological examination were also performed. Further, molecular docking studies were performed using ligand (−) epicatechin on human inducible nitric oxide synthase.

Results and conclusion

Phytochemically, EAC showed the presence of tannins, flavonoids, phenolic compounds, and saponins, while the content of (−) epicatechin was reported to be 7.81% w/w. The maximum healing of wounds (91.84 ± 1.10%) was observed in mice treated with a combination of both topical (10% gel) and oral (extract at 200 mg/kg) followed by topically and orally treated groups respectively after 14 days of treatment. These groups also showed significant restoration of altered biochemical parameters, antioxidant enzymes and cytokines. The molecular docking studies confirmed the role of (−) epicatechin in stabilizing the human inducible nitric oxide synthase with inhibitor showing binding energy of −8.31 kcal/mol. The present study confirmed the role of (−) epicatechin as a major marker in diabetic wound healing potential of A. catechu.

背景和目的儿茶野生Acacia catechu Wild。（蚕豆科）树皮传统上用于治疗糖尿病和伤口。因此，本研究的目的是评估儿茶醇提取物（EAC）对链脲佐菌素诱导的糖尿病小鼠的伤口愈合潜力。实验程序首先用高效液相色谱法，以（−）表儿茶素为标记物，对EAC进行了植物化学评价和标准化。使用链脲佐菌素在小鼠中诱导糖尿病，并使用局部和口服治疗的切除和切口伤口模型评估EAC的伤口愈合潜力。还进行了各种生化参数、体内抗氧化剂、细胞因子谱、VEGF和组织病理学检查。此外，使用配体（−）表儿茶素对人类诱导型一氧化氮合酶进行了分子对接研究。结果和结论EAC的植物化学成分中含有鞣质、黄酮类、酚类化合物和皂苷，而表儿茶素的含量为7.81%w/w。在用局部（10%凝胶）和口服（200mg/kg提取物）联合治疗的小鼠中观察到伤口的最大愈合率（91.84±1.10%），然后在治疗14天后分别用局部和口服治疗组。这些组还显示出改变的生化参数、抗氧化酶和细胞因子的显著恢复。分子对接研究证实了（−）表儿茶素在稳定人类诱导型一氧化氮合酶中的作用，其抑制剂显示出−8.31 kcal/mol的结合能。本研究证实了表儿茶素作为儿茶属糖尿病伤口愈合潜力的主要标志物的作用。

{"title":"Wound healing potential of Acacia catechu in streptozotocin-induced diabetic mice using in vivo and in silico approach","authors":"Vinayak P. Nakhate , Natasha S. Akojwar , Saurabh K. Sinha , Amarsinh D. Lomte , Mahaveer Dhobi , Prakash R. Itankar , Satyendra K. Prasad","doi":"10.1016/j.jtcme.2023.05.001","DOIUrl":"https://doi.org/10.1016/j.jtcme.2023.05.001","url":null,"abstract":"<div><h3>Background and aim</h3><p><em>Acacia catechu</em> Wild<strong>.</strong> (Fabaceae) barks are traditionally used in the treatment of diabetes and wounds. Therefore, the objective of the present study was to evaluate the wound healing potential of the alcoholic extract of <em>A. catechu</em> (EAC) in streptozotocin-induced diabetic mice.</p></div><div><h3>Experimental procedures</h3><p>EAC was first subjected to phytochemical estimations and standardization using (−) epicatechin as marker with the help of HPLC. Diabetes was induced in mice using streptozotocin and the wound healing potential of EAC was evaluated using excision and incision wound models on topical and oral treatment. Various biochemical parameters, <em>in vivo</em> antioxidants, cytokine profiling, VEGF, and histopathological examination were also performed. Further, molecular docking studies were performed using ligand (−) epicatechin on human inducible nitric oxide synthase.</p></div><div><h3>Results and conclusion</h3><p>Phytochemically, EAC showed the presence of tannins, flavonoids, phenolic compounds, and saponins, while the content of (−) epicatechin was reported to be 7.81% w/w. The maximum healing of wounds (91.84 ± 1.10%) was observed in mice treated with a combination of both topical (10% gel) and oral (extract at 200 mg/kg) followed by topically and orally treated groups respectively after 14 days of treatment. These groups also showed significant restoration of altered biochemical parameters, antioxidant enzymes and cytokines. The molecular docking studies confirmed the role of (−) epicatechin in stabilizing the human inducible nitric oxide synthase with inhibitor showing binding energy of −8.31 kcal/mol. The present study confirmed the role of (−) epicatechin as a major marker in diabetic wound healing potential of <em>A. catechu.</em></p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"13 5","pages":"Pages 489-499"},"PeriodicalIF":4.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49754878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Computational studies on searching potential phytochemicals against DNA polymerase activity of the monkeypox virus 寻找抗猴痘病毒DNA聚合酶活性的潜在植物化学物质的计算研究

IF 4.5 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2023-09-01 DOI: 10.1016/j.jtcme.2023.04.002

Seshu Vardhan, Suban K. Sahoo

Objectives

The outbreak of monkeypox virus (MPXV) is an emerging epidemic of medical concern with 65353 confirmed cases of infection and a fatality of 115 worldwide. Since May 2022, MPXV has been rapidly disseminating across the globe through various modes of transmission, including direct contact, respiratory droplets, and consensual sex. Because of the limited medical countermeasures available to treat MPXV, the present study aimed to identify potential phytochemicals (limonoids, triterpenoids, and polyphenols) as antagonists to target the DNA polymerase protein of MPXV with the ultimate goal to inhibit the viral DNA replication mechanism and immune-mediated responses.

Methods

The protein-DNA and protein-ligand molecular docking were performed with the help of computational programs AutoDock Vina, iGEMDOCK and HDOCK server. The BIOVIA Discovery studio and ChimeraX were used to evaluate the protein-ligand interactions. The GROMACS 2021 was used for the molecular dynamics simulations. The ADME and toxicity properties were computed by using online servers SwissADME and pKCSM.

Results

Molecular docking of 609 phytochemicals and molecular dynamics simulations of lead phytochemicals glycyrrhizinic acid and apigenin-7-O-glucuronide generated useful data that supported the ability of phytochemicals to obstruct the DNA polymerase activity of the monkeypox virus.

Conclusions

The computational results supported that appropriate phytochemicals can be used to formulate an adjuvant therapy for the monkeypox virus.

目的猴痘病毒（MPXV）的爆发是一种新出现的医学关注流行病，全球确诊感染病例65353例，死亡115例。自2022年5月以来，MPXV通过各种传播方式在全球迅速传播，包括直接接触、呼吸道飞沫和双方自愿的性行为。由于可用于治疗MPXV的药物对策有限，本研究旨在确定潜在的植物化学物质（柠檬苦素、三萜和多酚）作为靶向MPXV的DNA聚合酶蛋白的拮抗剂，最终目的是抑制病毒DNA复制机制和免疫介导的反应。方法利用AutoDock Vina、iGEMDOCK和HDOCK server程序进行蛋白质DNA和蛋白质配体分子对接。BIOVIA探索工作室和ChimeraX用于评估蛋白质与配体的相互作用。GROMACS 2021用于分子动力学模拟。使用在线服务器SwissADME和pKCSM计算ADME和毒性特性。结果609种植物化学物质的分子对接和主要植物化学物质甘草酸和芹菜素-7-O-葡糖苷酸的分子动力学模拟产生了有用的数据，支持了植物化学物质阻断猴痘病毒DNA聚合酶活性的能力。结论计算结果支持适当的植物化学物质可以用于猴痘病毒的辅助治疗。

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引用次数: 1

Exploring the effects and mechanisms of Guizhigancao Decoction on heart failure using an integrated approach based on experimental support and network pharmacology strategy 基于实验支持和网络药理学策略的综合研究桂枝甘草汤对心力衰竭的作用及机制

IF 4.5 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2023-09-01 DOI: 10.1016/j.jtcme.2023.03.010

Jianhua Qu , Jiao Wang , Biao Zheng , Xiaoxiao Jiang , Jikui Liu , Jing Chen

Background and aim

HF (Heart Failure) is the leading cause of mortality and is a significant clinical problem affecting millions of patients worldwide. To date, the mechanisms of HF remain largely elusive. The effective treatments contributing to HF remain incompletely understood. Therefore, the development of an effective strategy for HF is urgently needed.

Experimental procedure

In the present study, we devoted to investigating the effective treatments and sought to systematically decipher the related molecular mechanisms of Guizhigancao Decoction (GZGCD, Cinnamomum cassia Presl and Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle) for treating HF. We examined the therapeutic effect of GZGCD on HF in vivo. An integrative approach combining biomarker examination, echocardiography, myocardial fibrosis and cardiac apoptosis condition using Masson and TUNEL staining was performed to assess the efficacy of GZGCD against HF. Subsequently, comprehensive network pharmacology analyses were performed to explore the mechanisms involved in GZGCD therapeutic effects on HF.

Results and conclusions

The results showed that GZGCD could reverse cardiac function in rats with HF by reducing NT-proBNP, increasing EF, decreasing LVESV, LVEDV, LVIDs, LVIDd, increasing running time, and ameliorate myocardial collagen fiber hyperplasia and cardiomyocyte apoptosis. We showed that GZGCD might contribute to HF treatment via oxidative related pathways through bioinformatics. Eventually, promising compound quercetin in GZGCD for HF therapeutics was proposed in database-based analysis. Collectively, our findings indicate that GZGCD has a treatment effect on HF. We proposed that GZGCD might contribute HF treatment via oxidative response-related pathways.

背景和目的心力衰竭是导致死亡的主要原因，也是影响全球数百万患者的一个重大临床问题。到目前为止，HF的机制在很大程度上仍然难以捉摸。导致HF的有效治疗方法仍不完全清楚。因此，迫切需要制定一项有效的HF战略。实验方法本研究旨在探讨桂枝甘草汤治疗HF的有效方法，系统地揭示桂枝甘草汤、桂皮、甘草治疗HF的相关分子机制。采用Masson和TUNEL染色结合生物标志物检查、超声心动图、心肌纤维化和心肌细胞凋亡状况的综合方法来评估GZGCD对HF的疗效，结果与结论GZGCD可通过降低NT-proBNP、增加EF、降低LVESV、LVEDV、LVIDs、LVIDd、增加运行时间、，改善心肌胶原纤维增生和心肌细胞凋亡。我们通过生物信息学表明，GZGCD可能通过氧化相关途径对HF治疗有贡献。最终，在基于数据库的分析中，提出了GZGCD中有前景的化合物槲皮素用于HF治疗。总之，我们的研究结果表明GZGCD对HF有治疗作用。我们提出GZGCD可能通过氧化反应相关途径对HF治疗有贡献。

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引用次数: 0

Comparison and effect of moxibustion and acupuncture on Nogo/NgR signaling pathway in rats with cerebral ischemia/reperfusion injury 艾灸与针刺对脑缺血再灌注损伤大鼠Nogo/NgR信号通路的影响及比较

IF 4.5 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2023-09-01 DOI: 10.1016/j.jtcme.2023.03.006

You-jiang Min , Hai-hua Yao , Li Wang , Li-hong Cheng , En-si Hong

Background and aim

In China, acupuncture and moxibustion have been used effectively to treat various diseases for thousands of years. However, the evidence for a difference in the efficacies of moxibustion and acupuncture in cerebral infarction treatment is scarce. We aimed to compare the effects of acupuncture and moxibustion treatment on the Nogo/NgR signaling pathway in rats with cerebral ischemia/reperfusion (I/R) injury.

Experimental procedure

Eighty male SD rats were randomly divided into five groups, based on treatment received: sham surgery (sham group), middle cerebral artery occlusion (MCAO, MCAO group), MCAO and NEP(1–40) inhibitor injection (MCAO + block group), MCAO and moxibustion (MCAO + moxi group), and MCAO and minimal acupuncture (MCAO + MA group). Neurological status was evaluated before treatment, and cerebral infarction volume (IV) and neurological function; Nogo-A, NgR, p75NTR, and LINGO-1 expressions; and NgR and LINGO-1 co-expression were assessed after treatment.

Results and conclusion

After treatment, barring Nogo-A mRNA and protein expression in the MCAO + block group, the Longa score and IV significantly decreased; Nogo-A, NgR, p75NTR, and LINGO-1 mRNA and protein expressions as well as NgR and LINGO-1 co-expression significantly decreased in cerebral tissues; whereas the BWT score increased (P < 0.01) in the MCAO + moxi group, compared with the MCAO group. Except for NgR and LINGO-1 protein expressions, there were no significant differences in the abovementioned parameters between rats that underwent acupuncture and moxibustion. Acupuncture and moxibustion have similar effects on Nogo/NgR signaling pathway inhibition after cerebral infarction.

背景与目的在中国，针灸治疗各种疾病已有数千年的历史。然而，艾灸和针灸治疗脑梗死的疗效差异的证据很少。我们旨在比较针灸治疗对脑缺血/再灌注（I/R）损伤大鼠Nogo/NgR信号通路的影响。实验方法将80只雄性SD大鼠随机分为5组：假手术组（假手术组）、大脑中动脉闭塞组（MCAO、MCAO组）、MCAO和NEP（1-40）抑制剂注射组（MCAO+阻断组）、MCAO+艾灸组（MCAO+莫西组）、以及MCAO和微创针刺组（MCA0+MA组）。治疗前评估神经系统状况、脑梗死体积（IV）和神经功能；Nogo-A、NgR、p75NTR和LINGO-1表达；并在治疗后评估NgR和LINGO-1的共表达。结果与结论治疗后，MCAO+阻断组除Nogo-A mRNA和蛋白表达外，Longa评分和IV均显著下降；脑组织中Nogo-A、NgR、p75NTR和LINGO-1的mRNA和蛋白表达以及NgR和LINGO-1的共表达显著降低；而MCAO+莫西组的BWT评分与MCAO组相比增加（P＜0.01）。除NgR和LINGO-1蛋白表达外，针灸组大鼠上述参数无显著差异。针灸对脑梗死后Nogo/NgR信号通路的抑制作用相似。

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引用次数: 0

FM2- Aims & Scope FM2-目标和范围

IF 4.5 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2023-09-01 DOI: 10.1016/S2225-4110(23)00092-5

引用次数: 0

Barleria prionitis L. extracts ameliorate doxorubicin-induced acute kidney injury via modulation of oxidative stress, inflammation, and apoptosis 朊芽孢杆菌提取物通过调节氧化应激、炎症和细胞凋亡改善阿霉素诱导的急性肾损伤

IF 4.5 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2023-09-01 DOI: 10.1016/j.jtcme.2023.05.007

Sachinthi S. Amarasiri , Anoja P. Attanayake , Liyanage D.A.M. Arawwawala , Lakmini K.B. Mudduwa , Kamani A.P.W. Jayatilaka

Background and aim

Doxorubicin (DOX) is a chemotherapeutic drug with potential nephrotoxic effects on patients who are on cancer chemotherapy. An interest has been observed in using natural products to ameliorate the potential side effects of DOX. The present study is to investigate the cellular mechanisms underlying the protective effects of Barleria prionitis L. (BP) (Acanthaceae) extracts, DOX-induced acute kidney injury (AKI).

Experimental procedure

Hexane (25 mg/kg/day), ethyl acetate (80 mg/kg/day), n-butanol (70 mg/kg/day), and water (120 mg/kg/day) extracts of BP, were administered to DOX-induced (5 mg/kg (2500 μL/kg), ip) Wistar rats for four consecutive weeks. At the end of the study, investigations were carried out for the assessment of biomarkers of nephrotoxicity, oxidative stress, inflammation, and apoptosis.

Results

Treatments with BP extracts significantly reversed DOX-induced elevations in serum and urine biochemical markers of nephrotoxicity (serum creatinine; 21–33%, blood urea nitrogen; 26–58%, β₂-microglobulin; 19–22% and urine total protein; 47–67%). There was a reduction in the levels of tumor necrosis factor-α, interleukin-1β, and malondialdehyde in kidney homogenates of rats treated with the n-butanol extract (by 43, 62, and 24%) and water extract (by 57%, 85%, and 26%) (p < 0.05). Immunohistochemical expression of the pro-apoptotic B-cell associated X protein was reduced while the anti-apoptotic B-cell lymphoma gene product 2 protein was increased in kidney tissues after the treatments with BP extracts.

Conclusions

The selected BP extracts significantly ameliorated DOX-induced AKI. The findings would open new vistas for the development of a drug using the BP extracts to minimize DOX-induced AKI in cancer patients.

背景与目的阿霉素（DOX）是一种对癌症化疗患者具有潜在肾毒性的化疗药物。已经观察到人们对使用天然产品来改善DOX的潜在副作用感兴趣。本研究旨在探讨Barleria prionitis L.（BP）（无患子科）提取物、DOX诱导的急性肾损伤（AKI）保护作用的细胞机制，ip）Wistar大鼠连续4周。在研究结束时，对肾毒性、氧化应激、炎症和细胞凋亡的生物标志物进行了评估。结果BP提取物治疗可显著逆转DOX诱导的肾毒性血清和尿液生化标志物（血清肌酐；21-33%，血尿素氮；26-58%，β2-微球蛋白；19-22%，尿总蛋白；47-67%）的升高。肿瘤坏死因子-α、白细胞介素-1β、，以及用正丁醇提取物（增加43%、62%和24%）和水提取物（增加57%、85%和26%）处理的大鼠肾匀浆中的丙二醛（p＜0.05）。在用BP提取物处理后，肾组织中促凋亡B细胞相关X蛋白的免疫组化表达减少，而抗凋亡B细胞淋巴瘤基因产物2蛋白增加。结论所选BP提取物能显著改善DOX诱导的AKI。这一发现将为开发一种使用BP提取物最大限度减少癌症患者DOX诱导的AKI的药物开辟新的前景。

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引用次数: 1