Pub Date : 2023-12-21DOI: 10.20473/jfiki.v10i32023.379-385
Bida Cincin Kirana, Erlien Dwi Cahyani, Antonius Budiawan
Background: Indonesia is an equatorial country that is rich in sunlight all year. UV light is divided into three wavelength groups: UV-A (320-400nm), UV-B (280-320nm), and UV-C (100-290nm). Intracell chromophores in skin cell membranes such as riboflavin, porphyrin, nicotinamide, and enzymes will absorb the UV-A light. The UV-B light penetrates the dermis layer and causes DNA structure changes, which lead to wrinkles and a rising risk of skin cancer. Premature skin aging and skin cancer can be prevented with sunscreen preparation containing compounds that can protect the skin from UV radiation. Flavonoid is one of the purslane (Portulaca grandiflora) active metabolites that have the potency to be developed as sunscreen. Objective: This research aimed to determine the ability of purslane (Portulaca grandiflora) magenta flower variety herbs extract cream as a sunscreen as indicated by the %Te, %Tp, and Sun Protective Factor value. Methods: This research was an experimental study with various purslane magenta flower variety herbs extract cream formulas that were tested for their %Te, %Tp, and SPF value with a UV-Vis spectrophotometer. Results: The sunscreen cream preparation with 2.5% of purslane (Portulaca grandiflora) magenta flower variety herb extract had %Te, %Tp, and SPF values of 25.86 ± 2.41%, 36.05 ± 2.82%, and 3.97 ± 0.35 respectively. At the same time, preparations with 5% concentration of extract had %Te, %Tp, and SPF values of 8.23 ± 0.86%, 16.65 ± 0.92%, and 8.03 ± 0.38, respectively. Conclusion: The sunscreen activity of all extract concentration creams was significantly different compared to the negative control (cream base) in all parameters. Flavonoids are the compounds responsible for the sunscreen activity of purslane extract.
{"title":"Protective Factor Evaluation of Purslane (Portulaca grandiflora) Magenta Flower Variety Herbs Extract Cream Formula","authors":"Bida Cincin Kirana, Erlien Dwi Cahyani, Antonius Budiawan","doi":"10.20473/jfiki.v10i32023.379-385","DOIUrl":"https://doi.org/10.20473/jfiki.v10i32023.379-385","url":null,"abstract":"Background: Indonesia is an equatorial country that is rich in sunlight all year. UV light is divided into three wavelength groups: UV-A (320-400nm), UV-B (280-320nm), and UV-C (100-290nm). Intracell chromophores in skin cell membranes such as riboflavin, porphyrin, nicotinamide, and enzymes will absorb the UV-A light. The UV-B light penetrates the dermis layer and causes DNA structure changes, which lead to wrinkles and a rising risk of skin cancer. Premature skin aging and skin cancer can be prevented with sunscreen preparation containing compounds that can protect the skin from UV radiation. Flavonoid is one of the purslane (Portulaca grandiflora) active metabolites that have the potency to be developed as sunscreen. Objective: This research aimed to determine the ability of purslane (Portulaca grandiflora) magenta flower variety herbs extract cream as a sunscreen as indicated by the %Te, %Tp, and Sun Protective Factor value. Methods: This research was an experimental study with various purslane magenta flower variety herbs extract cream formulas that were tested for their %Te, %Tp, and SPF value with a UV-Vis spectrophotometer. Results: The sunscreen cream preparation with 2.5% of purslane (Portulaca grandiflora) magenta flower variety herb extract had %Te, %Tp, and SPF values of 25.86 ± 2.41%, 36.05 ± 2.82%, and 3.97 ± 0.35 respectively. At the same time, preparations with 5% concentration of extract had %Te, %Tp, and SPF values of 8.23 ± 0.86%, 16.65 ± 0.92%, and 8.03 ± 0.38, respectively. Conclusion: The sunscreen activity of all extract concentration creams was significantly different compared to the negative control (cream base) in all parameters. Flavonoids are the compounds responsible for the sunscreen activity of purslane extract.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138951450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-21DOI: 10.20473/jfiki.v10i32023.266-271
Faizal Hermanto, Aqila Refiani, Fahmi Ahsanul Haq
Background: Malaria is an infectious disease with a high mortality rate. One of the complications of malaria is blood disorders. Hematological disorders such as anemia and thrombocytopenia are common in malaria infection. Resveratrol has been reported to have antimalarial activity. Objective: This study aimed to evaluate the effect of resveratrol in reducing the severity of anemia and thrombocytopenia in Plasmodum berghei-infected mice. Methods: The study began with parasite inoculation in mice. After the mice were infected, and randomly grouped into negative control, chloroquine 20 mg/kg (positive control), and resveratrol with doses 25, 50, and 100 mg/kg. The mice's blood profile was measured on day 0 and day 4 using a hematology analyzer. Results: The results showed that after administration of resveratrol at various doses, the number of RBC, hemoglobin, hematocrit and platelets was higher than the control. Conclusion: It can be concluded that resveratrol can reduce the severity of anemia and thrombocytopenia in mice infected with P. berghei.
{"title":"Resveratrol Reduce the Severity of Anemia and Thrombocytopenia in Plasmodium berghei ANKA-Infected Mice","authors":"Faizal Hermanto, Aqila Refiani, Fahmi Ahsanul Haq","doi":"10.20473/jfiki.v10i32023.266-271","DOIUrl":"https://doi.org/10.20473/jfiki.v10i32023.266-271","url":null,"abstract":"Background: Malaria is an infectious disease with a high mortality rate. One of the complications of malaria is blood disorders. Hematological disorders such as anemia and thrombocytopenia are common in malaria infection. Resveratrol has been reported to have antimalarial activity. Objective: This study aimed to evaluate the effect of resveratrol in reducing the severity of anemia and thrombocytopenia in Plasmodum berghei-infected mice. Methods: The study began with parasite inoculation in mice. After the mice were infected, and randomly grouped into negative control, chloroquine 20 mg/kg (positive control), and resveratrol with doses 25, 50, and 100 mg/kg. The mice's blood profile was measured on day 0 and day 4 using a hematology analyzer. Results: The results showed that after administration of resveratrol at various doses, the number of RBC, hemoglobin, hematocrit and platelets was higher than the control. Conclusion: It can be concluded that resveratrol can reduce the severity of anemia and thrombocytopenia in mice infected with P. berghei.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138952103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-19DOI: 10.35814/jifi.v21i2.1406
Tias Eka Rahmawati, Agus Siswanto, Asmiyenti Djaliasrin Djalil
Diphenhydramine HCl is an antihistamine drug that is available in conventional tablet form. This study aimed to produce the optimum formula for a diphenhydramine fast disintegrating tablet (FDT) using a modification of starch, crospovidone, and microcrystalline cellulose (MCC) to produce quality tablets that meet the tablet's physical requirements and tablet dissolution. Starch modification was made using a two-step method of starch cross-link, then continued with silica coprecipitation. FDT was prepared by the direct compression method. Optimisation with the simplex lattice design (SLD) model uses three components: co-process starch crosslink-silica, crospovidone, and MCC, which obtained 14 formula designs. The hardness, wetting time, disintegration time, and percent dissolution are optimisation parameters. Equations, contour plots, and desirability values were determined as the optimum formula. Based on the research results, an optimum formula was obtained with the proportion of co-process cross-link starch-silica was 56.185 mg, crospovidone at 6 mg, and MCC at 45.815 mg. The result of hardness was 5 kg, wetting time 51.061 seconds, disintegration time 63.129 seconds, and dissolution was 100.972%. The interaction of the three components reduces hardness and increases disintegration time, wetting time, and percent dissolution.
{"title":"Optimization of Fast Disintegrating Tablets Diphenhydramine HCl using Co-process of Cross-link Yellow Kepok Banana Starch, Crospovidone, and Microcrystalline Cellulose","authors":"Tias Eka Rahmawati, Agus Siswanto, Asmiyenti Djaliasrin Djalil","doi":"10.35814/jifi.v21i2.1406","DOIUrl":"https://doi.org/10.35814/jifi.v21i2.1406","url":null,"abstract":"Diphenhydramine HCl is an antihistamine drug that is available in conventional tablet form. This study aimed to produce the optimum formula for a diphenhydramine fast disintegrating tablet (FDT) using a modification of starch, crospovidone, and microcrystalline cellulose (MCC) to produce quality tablets that meet the tablet's physical requirements and tablet dissolution. Starch modification was made using a two-step method of starch cross-link, then continued with silica coprecipitation. FDT was prepared by the direct compression method. Optimisation with the simplex lattice design (SLD) model uses three components: co-process starch crosslink-silica, crospovidone, and MCC, which obtained 14 formula designs. The hardness, wetting time, disintegration time, and percent dissolution are optimisation parameters. Equations, contour plots, and desirability values were determined as the optimum formula. Based on the research results, an optimum formula was obtained with the proportion of co-process cross-link starch-silica was 56.185 mg, crospovidone at 6 mg, and MCC at 45.815 mg. The result of hardness was 5 kg, wetting time 51.061 seconds, disintegration time 63.129 seconds, and dissolution was 100.972%. The interaction of the three components reduces hardness and increases disintegration time, wetting time, and percent dissolution.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135824299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-19DOI: 10.35814/jifi.v21i2.1385
Muhammad Alfian, Nining Sugihartini, Sapto Yuliani
This research aimed to evaluate how enhancers affect the properties of Syzygium aromaticum oil in both water-soluble and hydrocarbon bases. Physical and chemical characteristic tests, irritation index evaluations, and anti-inflammatory activity assessments were carried out to analyze the Syzygium aromaticum oil ointment. The pH tests were conducted to measure the chemical characteristics and viscosity, adhesivity, spreadability tests were conducted to measure the physical characteristics of the ointment. Rabbit-based tests were used to determine the irritation index, mice induced by crotton oil were used in anti-inflammatory activity assessments. The pH tests revealed no significant differences between both of ointments. The ointment in the water-soluble base had higher viscosity and adhesivity but lower spreadability than the ointment in the hydrocarbon base. The epidermis thickness of watersoluble and hydrocarbon-based ointments was 3.81±0.40 μm and 4.22±0.15 μm, while the number of cells with COX-2 expression was 18.24±6.51 and 21.01±5.96. The addition of enhancers did not cause any irritation in either ointment. The study concluded that the formula of Syzygium aromaticum oil in a water-soluble ointment with the addition of propylene glycol and oleic acid (70:30) as enhancers provided better physical and chemical characteristics, a lower irritation index, and higher anti-inflammatory activity compared to the hydrocarbon.
{"title":"Physical and Chemical Characteristic, Irritation Index and Anti-inflammatory Activity from Ointment of Syzygium aromaticum Oil by Adding an Enhancer","authors":"Muhammad Alfian, Nining Sugihartini, Sapto Yuliani","doi":"10.35814/jifi.v21i2.1385","DOIUrl":"https://doi.org/10.35814/jifi.v21i2.1385","url":null,"abstract":"This research aimed to evaluate how enhancers affect the properties of Syzygium aromaticum oil in both water-soluble and hydrocarbon bases. Physical and chemical characteristic tests, irritation index evaluations, and anti-inflammatory activity assessments were carried out to analyze the Syzygium aromaticum oil ointment. The pH tests were conducted to measure the chemical characteristics and viscosity, adhesivity, spreadability tests were conducted to measure the physical characteristics of the ointment. Rabbit-based tests were used to determine the irritation index, mice induced by crotton oil were used in anti-inflammatory activity assessments. The pH tests revealed no significant differences between both of ointments. The ointment in the water-soluble base had higher viscosity and adhesivity but lower spreadability than the ointment in the hydrocarbon base. The epidermis thickness of watersoluble and hydrocarbon-based ointments was 3.81±0.40 μm and 4.22±0.15 μm, while the number of cells with COX-2 expression was 18.24±6.51 and 21.01±5.96. The addition of enhancers did not cause any irritation in either ointment. The study concluded that the formula of Syzygium aromaticum oil in a water-soluble ointment with the addition of propylene glycol and oleic acid (70:30) as enhancers provided better physical and chemical characteristics, a lower irritation index, and higher anti-inflammatory activity compared to the hydrocarbon.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135824297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-19DOI: 10.35814/jifi.v21i2.1486
Nina Salamah, Arum Fatmawati, Any Guntarti
Gelatin is widely used in the food industry, including soft candy. It is generally sourced from cows and pigs. However, adding porcine gelatin to food will be problematic for the Muslim community due to its prohibition in their beliefs. Therefore, the soft candy’s components should be analysed to determine whether the functional groups are of bovine or porcine gelatin. From three local soft candy products in Indonesia, gelatin was isolated with acetone by vortexing and centrifugation. The supernatant was analysed with FTIR to determine its spectral profile. Furthermore, the wavenumbers and absorbances of gelatin’s functional groups were analysed in Minitab 18 with multivariate PLS and PCA. FTIR showed that gelatin contained proteins with O-H, aliphatic C-H, C=O, N-H, and C-N groups. PLS and PCA were conducted in the wavenumber range of 1576–1481 cm-1. The calibration yielded R2 = 0.9997 and RMSEC = 0.9376%. The internal validation showed R2 = 0.9998 and RMSECV = 1.29%, while the external validation produced R2 = 0.9996 and RMSEP = 1.04%. Clustering with PCA revealed that the gelatin sample from one soft candy was in the same quadrant as the reference bovine gelatin, while the other two were in different quadrants from both references.
{"title":"Gelatin Analysis in Local Soft Candy Products using Fourier Transform Infrared (ATR-FTIR) Combined with Chemometrics","authors":"Nina Salamah, Arum Fatmawati, Any Guntarti","doi":"10.35814/jifi.v21i2.1486","DOIUrl":"https://doi.org/10.35814/jifi.v21i2.1486","url":null,"abstract":"Gelatin is widely used in the food industry, including soft candy. It is generally sourced from cows and pigs. However, adding porcine gelatin to food will be problematic for the Muslim community due to its prohibition in their beliefs. Therefore, the soft candy’s components should be analysed to determine whether the functional groups are of bovine or porcine gelatin. From three local soft candy products in Indonesia, gelatin was isolated with acetone by vortexing and centrifugation. The supernatant was analysed with FTIR to determine its spectral profile. Furthermore, the wavenumbers and absorbances of gelatin’s functional groups were analysed in Minitab 18 with multivariate PLS and PCA. FTIR showed that gelatin contained proteins with O-H, aliphatic C-H, C=O, N-H, and C-N groups. PLS and PCA were conducted in the wavenumber range of 1576–1481 cm-1. The calibration yielded R2 = 0.9997 and RMSEC = 0.9376%. The internal validation showed R2 = 0.9998 and RMSECV = 1.29%, while the external validation produced R2 = 0.9996 and RMSEP = 1.04%. Clustering with PCA revealed that the gelatin sample from one soft candy was in the same quadrant as the reference bovine gelatin, while the other two were in different quadrants from both references.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135824298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-16DOI: 10.35814/jifi.v21i2.1447
Manuel Hutapea, Greesty Finotory Swandiny, M. Irfan Syafawi, Fahreza Pratama Putra, Sucipto Kokadir, Edward Basilianus, Vinessa Gracia Putri, Syamsudin Abdillah
Sea cucumbers are deep-sea-dwelling invertebrate animals that are rich in nutrients and have been empirically used to prevent various degenerative diseases, especially due to their ability to dampen free radicals. The purpose of this study was to identify, and determine antioxidant activity, total phenolic content, and toxic properties in extracts of several sea cucumber species originating from Tegal Mas Island, Lampung. The identification results of the three sea cucumber species were Stichopus vastus Sluiter (SV), Stichopus monotuberculatus Quay & Gaimard (SM), and Stichopus quadrifasciatus Massin. (SQ). The extraction was performed by kinetic maceration against all parts of the sea cucumber using a 70% ethanol solvent. Antioxidant activity test using the DPPH (2,2-diphenylpicrilhydrazyl) free radical suppression method and determination of the total phenolic content using the Folin-Ciocalteu method. The results showed that SV had the highest total phenolics and antioxidants with the lowest toxicity. However, optimisation of extraction between wet and dry sea cucumbers is still required. Extraction optimisation with various solvents can also be done to gain secondary metabolites more effectively.
{"title":"The Latest Research on 3 (Three) Types of Sea Cucumber Extract from Tegal Mas Island, Lampung","authors":"Manuel Hutapea, Greesty Finotory Swandiny, M. Irfan Syafawi, Fahreza Pratama Putra, Sucipto Kokadir, Edward Basilianus, Vinessa Gracia Putri, Syamsudin Abdillah","doi":"10.35814/jifi.v21i2.1447","DOIUrl":"https://doi.org/10.35814/jifi.v21i2.1447","url":null,"abstract":"Sea cucumbers are deep-sea-dwelling invertebrate animals that are rich in nutrients and have been empirically used to prevent various degenerative diseases, especially due to their ability to dampen free radicals. The purpose of this study was to identify, and determine antioxidant activity, total phenolic content, and toxic properties in extracts of several sea cucumber species originating from Tegal Mas Island, Lampung. The identification results of the three sea cucumber species were Stichopus vastus Sluiter (SV), Stichopus monotuberculatus Quay & Gaimard (SM), and Stichopus quadrifasciatus Massin. (SQ). The extraction was performed by kinetic maceration against all parts of the sea cucumber using a 70% ethanol solvent. Antioxidant activity test using the DPPH (2,2-diphenylpicrilhydrazyl) free radical suppression method and determination of the total phenolic content using the Folin-Ciocalteu method. The results showed that SV had the highest total phenolics and antioxidants with the lowest toxicity. However, optimisation of extraction between wet and dry sea cucumbers is still required. Extraction optimisation with various solvents can also be done to gain secondary metabolites more effectively.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-16DOI: 10.35814/jifi.v21i2.1473
Faizatun Faizatun, Irene Intan Permata Murti
Mulberry is a plant that has been shown to be effective as a whitening agent. The objective of this research was to evaluate the efficacy of mulberry root extract (MRE) and its nanostructured lipid carrier (NLC) extracts in terms of their ability to lighten skin tone by using zebrafish as a model. NLC were formulated into gel preparations. The whitening effect of MRE and NLC was evaluated, and the melanin content of the extracts was found to be the result of the evaluation. Based on the results of morphological observations, a decrease in melanin levels was seen in the eye. In the mulberry root extract, the melanin level decreased with an increase in the concentration of the mulberry root extract. In the NLC of mulberry root extract, a decrease in melanin levels was obtained at an increased NLC concentration of mulberry root extract. It can be concluded that mulberry root NLC has the ability to act as a whitening agent, which is distinguished by the reduction of melanin content.
{"title":"Formulation of Nanostructured Lipid Carrier Gel From Mulberry Root Extract (Morus alba L.) as Whitening Agent using Zebrafish Modelling","authors":"Faizatun Faizatun, Irene Intan Permata Murti","doi":"10.35814/jifi.v21i2.1473","DOIUrl":"https://doi.org/10.35814/jifi.v21i2.1473","url":null,"abstract":"Mulberry is a plant that has been shown to be effective as a whitening agent. The objective of this research was to evaluate the efficacy of mulberry root extract (MRE) and its nanostructured lipid carrier (NLC) extracts in terms of their ability to lighten skin tone by using zebrafish as a model. NLC were formulated into gel preparations. The whitening effect of MRE and NLC was evaluated, and the melanin content of the extracts was found to be the result of the evaluation. Based on the results of morphological observations, a decrease in melanin levels was seen in the eye. In the mulberry root extract, the melanin level decreased with an increase in the concentration of the mulberry root extract. In the NLC of mulberry root extract, a decrease in melanin levels was obtained at an increased NLC concentration of mulberry root extract. It can be concluded that mulberry root NLC has the ability to act as a whitening agent, which is distinguished by the reduction of melanin content.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-16DOI: 10.35814/jifi.v21i2.1282
Nurita Andayani, Raise Maninda, Rosa Marlina, Qoina Noviantani, Khaled Qalalwa
Mycobacterium tuberculosis causes Tuberculosis (TB), an infectious disease. The study aims to evaluate DOTS therapy's impact on pulmonary TB and TB-HIV patients. This research used a descriptive qualitative study design with a retrospective descriptive research approach using secondary data for the period June 2017 to July 2020. The analysis uses the Miles and Huberman model. A study of 305 people found that 83% of pulmonary TB cases completed treatment within the initial phase, 64% completed the continuation phase. TB-HIV cases 93% completed treatment within the initial phase, 71% the continuation phase. The combination of OAT and ARV doses was successful in reducing initial smear conversion in pulmonary TB cases. The treatment was evaluated as cure in pulmonary TB cases, complete in pulmonary TB-HIV cases, and had no treatment failure status in both groups. Treatment success in pulmonary TB patients was not significantly related (p-value≥5%) with the type of OAT, type of PMO, gender, and age. Whereas in HIV co-infected pulmonary TB patients, treatment success was significantly related (p-value <5%) with the type of PMO, and not related (p-value≥5%) with the type of OAT, gender, and age.
{"title":"The Analysis of The Effectiveness of Pulmonary Tuberculosis and HIV-Co-TB Treatment Therapies at Sulianti Saroso Infectious Diseases Hospital","authors":"Nurita Andayani, Raise Maninda, Rosa Marlina, Qoina Noviantani, Khaled Qalalwa","doi":"10.35814/jifi.v21i2.1282","DOIUrl":"https://doi.org/10.35814/jifi.v21i2.1282","url":null,"abstract":"Mycobacterium tuberculosis causes Tuberculosis (TB), an infectious disease. The study aims to evaluate DOTS therapy's impact on pulmonary TB and TB-HIV patients. This research used a descriptive qualitative study design with a retrospective descriptive research approach using secondary data for the period June 2017 to July 2020. The analysis uses the Miles and Huberman model. A study of 305 people found that 83% of pulmonary TB cases completed treatment within the initial phase, 64% completed the continuation phase. TB-HIV cases 93% completed treatment within the initial phase, 71% the continuation phase. The combination of OAT and ARV doses was successful in reducing initial smear conversion in pulmonary TB cases. The treatment was evaluated as cure in pulmonary TB cases, complete in pulmonary TB-HIV cases, and had no treatment failure status in both groups. Treatment success in pulmonary TB patients was not significantly related (p-value≥5%) with the type of OAT, type of PMO, gender, and age. Whereas in HIV co-infected pulmonary TB patients, treatment success was significantly related (p-value <5%) with the type of PMO, and not related (p-value≥5%) with the type of OAT, gender, and age.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-16DOI: 10.35814/jifi.v21i2.1387
Andri Prasetiyo, Wiwi Winarti, Rini Agustia
People have long utilised the leaves of the red gedi, Abelmoschus manihot (L.) Medik is an anti-diabetic medication. The study’s objectives were to classify chemical constituent groups, assess extract quality, and evaluate the activity of α-glucosidase enzyme inhibitors. The extraction method employed kinetic maceration with a 70% ethanol solvent, identification was accomplished through phytochemical screening, a quality determination was based on both specific and non-specific parameters, and α-glucosidase inhibition was used in an in vitro antidiabetic activity test. Flavonoids, alkaloids, saponins, steroids, and triterpenoids were found in the phytochemical screening test results. The results of the quality parameter test obtained viscous extract, blackish green color, 70.58% water soluble extract, 49.46% ethanol soluble extract, 7.82% loss on drying, 4.19% water content, total ash content 6.97%, acid insoluble ash content 0.60%, water-soluble ash content 5.64%, residual solvent 0.76%, heavy metal content Pb 1.35 mg/kg, Cd 0.06 mg/kg, plate number total ≤10 colonies/g, yeast plate number 20.1995 colonies/g and total flavonoid content 1.20%. The α-glucosidase enzyme inhibitory activity test from the extract and acarbose (positive controls) obtained IC50 values of 81.84 μg/mL and 42.6 μg/mL, respectively. In conclusion, the α-glucosidase enzyme may be inhibited by the ethanolic extract of A. manihot (L.) Medik leaves.
长期以来，人们一直利用红葛地的叶子，Abelmoschus manihot。Medik是一种抗糖尿病药物。该研究的目的是分类化学成分组，评价提取物的质量，并评价α-葡萄糖苷酶抑制剂的活性。提取方法采用70%乙醇溶剂动力学浸渍法，通过植物化学筛选鉴定，通过特异性和非特异性参数确定质量，并采用α-葡萄糖苷酶抑制法进行体外抗糖尿病活性试验。筛选结果发现黄酮类、生物碱类、皂苷类、甾体类和三萜类化合物。质量参数试验结果得到:浸出物粘稠度高，色泽墨绿色，水溶性浸出物70.58%，乙醇可溶性浸出物49.46%，干燥损失率7.82%，含水量4.19%，总灰分6.97%，酸不溶灰分0.60%，水溶性灰分5.64%，残溶剂0.76%，重金属含量Pb 1.35 mg/kg, Cd 0.06 mg/kg，菌落总数≤10菌落/g，酵母菌落数20.1995菌落/g，总黄酮含量1.20%。提取物α-葡萄糖苷酶抑制活性试验和阳性对照阿卡波糖的IC50值分别为81.84 μg/mL和42.6 μg/mL。综上所述，马尼霍特(L. manhot)乙醇提取物可能对α-葡萄糖苷酶有抑制作用。Medik树叶。
{"title":"The inhibition of α-Glucosidase enzyme activity from Standardised Ethanol Extract of Abelmoschus manihot (L.) Medik Leaves","authors":"Andri Prasetiyo, Wiwi Winarti, Rini Agustia","doi":"10.35814/jifi.v21i2.1387","DOIUrl":"https://doi.org/10.35814/jifi.v21i2.1387","url":null,"abstract":"People have long utilised the leaves of the red gedi, Abelmoschus manihot (L.) Medik is an anti-diabetic medication. The study’s objectives were to classify chemical constituent groups, assess extract quality, and evaluate the activity of α-glucosidase enzyme inhibitors. The extraction method employed kinetic maceration with a 70% ethanol solvent, identification was accomplished through phytochemical screening, a quality determination was based on both specific and non-specific parameters, and α-glucosidase inhibition was used in an in vitro antidiabetic activity test. Flavonoids, alkaloids, saponins, steroids, and triterpenoids were found in the phytochemical screening test results. The results of the quality parameter test obtained viscous extract, blackish green color, 70.58% water soluble extract, 49.46% ethanol soluble extract, 7.82% loss on drying, 4.19% water content, total ash content 6.97%, acid insoluble ash content 0.60%, water-soluble ash content 5.64%, residual solvent 0.76%, heavy metal content Pb 1.35 mg/kg, Cd 0.06 mg/kg, plate number total ≤10 colonies/g, yeast plate number 20.1995 colonies/g and total flavonoid content 1.20%. The α-glucosidase enzyme inhibitory activity test from the extract and acarbose (positive controls) obtained IC50 values of 81.84 μg/mL and 42.6 μg/mL, respectively. In conclusion, the α-glucosidase enzyme may be inhibited by the ethanolic extract of A. manihot (L.) Medik leaves.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-16DOI: 10.35814/jifi.v21i2.1440
I Gusti Agung Ayu Kartika, Suryani Suryani, Dwi Hadi Setya Palupi, I Ketut Adnyana
Sirih bumi (Peperomia pellucida [L.] Kunth.) is a medicinal herb. Anti-inflammatory activities are reported. These plant extracts have been shown to reduce inflammation in many studies, but it is unknown which extract works best. The objectives if this study were determine the extract that has the best anti-inflammatory activity from sirih bumi. The activity was assessed from the impact of stabilising red blood cell membranes in vitro. The research was then continued with a literature study on the chemical compounds containing the best extracts. To determine the prediction of the compound responsible for its activity and potency, several in silico tests were carried out, such as PASS online, Pre-ADMET online, and Protox II. The test results showed that the ethyl acetate extract at 200 g/mL concentration showed similar activity to aspirin at the same concentration. The activity of this extract increased with increasing concentration of the extract. Six compounds were reported to be isolated from this type of extract. Dillapiol and its derivative 6-allyl-5-methoxy-1,3-benzodioxol-4-ol are predicted to be compounds that have anti-inflammatory effects by inhibiting the membrane permeability mechanism of ethyl acetate extract. Ethyl acetate extract showed the best anti-inflammatory activity among the other type of extract from sirih bumi. Ethyl acetate extract is a type of extract with the best anti-inflammatory activity from sirih bumi.
{"title":"Stabilisation Potential of Cell Membrane from Different Polarity Extract of Sirih Bumi (Peperomia pellucida [L.] Kunth) as Anti-inflammatory Agent","authors":"I Gusti Agung Ayu Kartika, Suryani Suryani, Dwi Hadi Setya Palupi, I Ketut Adnyana","doi":"10.35814/jifi.v21i2.1440","DOIUrl":"https://doi.org/10.35814/jifi.v21i2.1440","url":null,"abstract":"Sirih bumi (Peperomia pellucida [L.] Kunth.) is a medicinal herb. Anti-inflammatory activities are reported. These plant extracts have been shown to reduce inflammation in many studies, but it is unknown which extract works best. The objectives if this study were determine the extract that has the best anti-inflammatory activity from sirih bumi. The activity was assessed from the impact of stabilising red blood cell membranes in vitro. The research was then continued with a literature study on the chemical compounds containing the best extracts. To determine the prediction of the compound responsible for its activity and potency, several in silico tests were carried out, such as PASS online, Pre-ADMET online, and Protox II. The test results showed that the ethyl acetate extract at 200 g/mL concentration showed similar activity to aspirin at the same concentration. The activity of this extract increased with increasing concentration of the extract. Six compounds were reported to be isolated from this type of extract. Dillapiol and its derivative 6-allyl-5-methoxy-1,3-benzodioxol-4-ol are predicted to be compounds that have anti-inflammatory effects by inhibiting the membrane permeability mechanism of ethyl acetate extract. Ethyl acetate extract showed the best anti-inflammatory activity among the other type of extract from sirih bumi. Ethyl acetate extract is a type of extract with the best anti-inflammatory activity from sirih bumi.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：