Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder. Parameters characteristic of the disorder include weight gain, insulin resistance and malondialdehyde (MDA). The traditional medicine used is Moringa oleifera. Objectives: The aim was to determine the effect of weight loss and blood glucose levels, MDA levels and histopathological features of the ovarian follicles in the rat model PCOS-insulin resistance induced by testosterone. Methods: Extraction process, followed by fractionation using n-hexane, ethyl acetate and water, identification of compound content using TLC, and rats were grouped into 7 groups (n=5), namely normal group, negative group, positive group, extract, fraction water, ethyl acetate fraction and n-hexane fraction by looking at the characteristic parameters and ovarian histopathology. Data analysis using ANOVA and Kruskal-wallis. Results: The yield of the extract was 30.4%, the water fraction was 85.59%, the ethyl acetate fraction was 6.64% and the n-hexane fraction was 4.05%. Positive for flavonoids, tannins, alkaloids in the ethyl acetate fraction, the water and extract fractions were positive for tannins, the n-hexane fraction was positive for steroids. The modeling sample group obtained extract body weight 195.40 g, water fraction 195.80 g, ethyl acetate fraction 194.00 g, n-hexane fraction 196.00 g, blood glucose level extract 83.00 mg/dL, water fraction 84.27 mg/dL, ethyl acetate fraction 80.00 mg/dL, n-hexane fraction 122.85 mg/dL, MDA extract content 2.704 nmol/mL, water fraction 3.547 nmol/mL, 1.685 nmol/mL, 5.308 nmol /mL and can improve ovarian histopathology. Conclusion: The most effective value is the ethyl acetate fraction because it has the highest decrease in PCOS characteristics.
{"title":"In vivo Evaluation of Extracted and Fraction of Moringa oleifera leaves against Testosterone-Induced PCOS Model in Rattus Norvegicus","authors":"Widayatul Khairi, None Nuraini Harmastuti, None Gunawan Pamudji Widodo","doi":"10.20473/jfiki.v10i22023.224-234","DOIUrl":"https://doi.org/10.20473/jfiki.v10i22023.224-234","url":null,"abstract":"Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder. Parameters characteristic of the disorder include weight gain, insulin resistance and malondialdehyde (MDA). The traditional medicine used is Moringa oleifera. Objectives: The aim was to determine the effect of weight loss and blood glucose levels, MDA levels and histopathological features of the ovarian follicles in the rat model PCOS-insulin resistance induced by testosterone. Methods: Extraction process, followed by fractionation using n-hexane, ethyl acetate and water, identification of compound content using TLC, and rats were grouped into 7 groups (n=5), namely normal group, negative group, positive group, extract, fraction water, ethyl acetate fraction and n-hexane fraction by looking at the characteristic parameters and ovarian histopathology. Data analysis using ANOVA and Kruskal-wallis. Results: The yield of the extract was 30.4%, the water fraction was 85.59%, the ethyl acetate fraction was 6.64% and the n-hexane fraction was 4.05%. Positive for flavonoids, tannins, alkaloids in the ethyl acetate fraction, the water and extract fractions were positive for tannins, the n-hexane fraction was positive for steroids. The modeling sample group obtained extract body weight 195.40 g, water fraction 195.80 g, ethyl acetate fraction 194.00 g, n-hexane fraction 196.00 g, blood glucose level extract 83.00 mg/dL, water fraction 84.27 mg/dL, ethyl acetate fraction 80.00 mg/dL, n-hexane fraction 122.85 mg/dL, MDA extract content 2.704 nmol/mL, water fraction 3.547 nmol/mL, 1.685 nmol/mL, 5.308 nmol /mL and can improve ovarian histopathology. Conclusion: The most effective value is the ethyl acetate fraction because it has the highest decrease in PCOS characteristics.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135066119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-02DOI: 10.20473/jfiki.v10i22023.193-201
Fathul Muin, Anna Wahyuni Widayanti
Background: Dispensing antibiotics without a prescription at community pharmacies is a significant contributor to the ongoing global public health issue of antibiotic resistance. Objectives: To estimate the proportion of antibiotics that are dispensed without a prescription in community pharmacies in various Indonesian cities. Methods: A literature review was conducted via PubMed, Science Direct, Google Scholar, Garuda, and Neliti for articles published between January 2007 and December 2022 combined with Boolean operators. The literature search keywords were (simulated patientsOR mystery shopper OR sample patients OR dummy patients) AND ("antibiotics without prescription OR non-prescription antibiotics OR self-medication of antibiotics). The keywords are also used in Indonesian language (Bahasa), including “simulasi pasien” OR “sampel pasien” AND “antibiotik tanpa resep” OR “swamedikasi antibiotik”. Results: Seven studies from various cities have complied with the inclusion criteria and were considered when reviewing 199 articles. The findings of our studies were consistent with the extensive use of non-prescription antibiotics throughout the review. A simulation patient study design was used in all seven studies in this review. Amoxicillin recorded the highest percentage of dispensing without a prescription, while other drugs often purchased include chloramphenicol, ciprofloxacin, and cefadroxil. Among the studies reviewed, one study utilized the pre-test and post-test methods, while the others did not. Conclusion: The lack of prescriptions for antibiotics dispensing has often occurred in community pharmacies throughout Indonesia. The community pharmacist's role is needed as the final gate of pharmaceutical services in providing rational treatment and controlling the dispense of antibiotics without a prescription.
背景:在社区药房无处方分发抗生素是造成抗生素耐药性这一持续的全球公共卫生问题的一个重要因素。目的:估计印度尼西亚各城市社区药房无处方分配抗生素的比例。方法:结合布尔运算符，通过PubMed、Science Direct、Google Scholar、Garuda和Neliti对2007年1月至2022年12月间发表的文章进行文献综述。文献检索关键词为(模拟患者或神秘购物者或样本患者或虚拟患者)和(无处方抗生素或非处方抗生素或自行用药抗生素)。这些关键词也用在印尼语中，包括“simulasi pasien”或“sampel pasien”和“antibiotic tik tanpa resep”或“swamedikasi antibiotic tik”。结果:来自不同城市的7项研究符合纳入标准，共纳入199篇文献。我们的研究结果与整个综述中非处方抗生素的广泛使用一致。本综述的所有7项研究均采用模拟患者研究设计。阿莫西林在没有处方的情况下配药的比例最高，而其他经常购买的药物包括氯霉素、环丙沙星和头孢醚。在回顾的研究中，一项研究使用了前测和后测方法，而其他研究则没有。结论:印度尼西亚社区药房经常出现抗生素配药处方不足的情况。社区药师作为药学服务的最后一扇门，在提供合理治疗和控制无处方抗生素配药方面发挥着重要作用。
{"title":"Using Simulated Patients to Understand Non-Prescription Antibiotic Dispensing in Indonesia: A Systematic Review","authors":"Fathul Muin, Anna Wahyuni Widayanti","doi":"10.20473/jfiki.v10i22023.193-201","DOIUrl":"https://doi.org/10.20473/jfiki.v10i22023.193-201","url":null,"abstract":"Background: Dispensing antibiotics without a prescription at community pharmacies is a significant contributor to the ongoing global public health issue of antibiotic resistance. Objectives: To estimate the proportion of antibiotics that are dispensed without a prescription in community pharmacies in various Indonesian cities. Methods: A literature review was conducted via PubMed, Science Direct, Google Scholar, Garuda, and Neliti for articles published between January 2007 and December 2022 combined with Boolean operators. The literature search keywords were (simulated patientsOR mystery shopper OR sample patients OR dummy patients) AND (\"antibiotics without prescription OR non-prescription antibiotics OR self-medication of antibiotics). The keywords are also used in Indonesian language (Bahasa), including “simulasi pasien” OR “sampel pasien” AND “antibiotik tanpa resep” OR “swamedikasi antibiotik”. Results: Seven studies from various cities have complied with the inclusion criteria and were considered when reviewing 199 articles. The findings of our studies were consistent with the extensive use of non-prescription antibiotics throughout the review. A simulation patient study design was used in all seven studies in this review. Amoxicillin recorded the highest percentage of dispensing without a prescription, while other drugs often purchased include chloramphenicol, ciprofloxacin, and cefadroxil. Among the studies reviewed, one study utilized the pre-test and post-test methods, while the others did not. Conclusion: The lack of prescriptions for antibiotics dispensing has often occurred in community pharmacies throughout Indonesia. The community pharmacist's role is needed as the final gate of pharmaceutical services in providing rational treatment and controlling the dispense of antibiotics without a prescription.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135064826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Diabetes mellitus is a chronic metabolic disorder that cannot be cured and progressively develop into a complication affecting the patient's health-related quality of life (HRQoL). The utility is a measure of preference-based HRQoL. Indirect utility measurements can be measured using the Multi-Attribute Utility Instrument (MAUI) instrument, one of which is the 15D questionnaire that has never been translated and adapted culturally in Indonesian. Objective: Translating and culturally adapting the 15D questionnaire to Indonesian diabetes mellitus patients. Methods: The translation process was carried out through the stages of forward translation, reconciliation, and backward translation involving two qualified translators and three experts in their field. The pilot test stage involved eight respondents consisting of 6 diabetes mellitus patients and two healthy individuals. Results: Problems in the linguistic validation process led to more conformity of word equivalents from the original to the target language. The agreement found was in the area of semantic equivalence, idiomatic equivalence, and experiential equivalence. The problem was resolved by reconciliation during the Focus Group Discussion, which translators and experts in their field attended and discussed with the original author to get equality of meaning in terms of language and culture. The Indonesian version of the 15D questionnaire tested on eight respondents showed results that were easy to understand and straightforward. Conclusion: The Indonesian version of the 15D questionnaire is valid from the linguistic and cultural adaptation stage. Further research is needed relating to the validation and reliability of the questionnaire.
背景:糖尿病是一种无法治愈的慢性代谢性疾病，并逐渐发展为影响患者健康相关生活质量的并发症。实用程序是基于偏好的HRQoL的度量。间接效用测量可以使用多属性效用工具(MAUI)来测量，其中之一是从未在印度尼西亚翻译和适应文化的15D问卷。目的:对印尼糖尿病患者的15D问卷进行翻译和文化适应。方法:由2名合格的翻译人员和3名相关领域的专家，通过正向翻译、调和翻译和反向翻译三个阶段进行翻译过程。中试阶段共8人，包括6名糖尿病患者和2名健康人。结果:语言验证过程中存在的问题导致原语与译语的对等词更加符合。发现的一致是在语义对等、习惯对等和经验对等领域。在Focus Group Discussion中，译者和各自领域的专家参与并与原作者讨论，以达到语言和文化意义上的平等，通过和解解决了这个问题。印尼版的15D问卷对8名受访者进行了测试，结果很容易理解和直接。结论:印尼语版的15D问卷在语言和文化适应阶段是有效的。问卷的效度和信度有待进一步研究。
{"title":"Translation and Cultural Adaptation of Multi-Attribute Utility Instrument (MAUI) Indonesian Version of the 15D Questionnaire","authors":"None Dian Parwati, None Libriansyah, None Gesnita Nugraheni, None Yunita Nita","doi":"10.20473/jfiki.v10i22023.162-172","DOIUrl":"https://doi.org/10.20473/jfiki.v10i22023.162-172","url":null,"abstract":"Background: Diabetes mellitus is a chronic metabolic disorder that cannot be cured and progressively develop into a complication affecting the patient's health-related quality of life (HRQoL). The utility is a measure of preference-based HRQoL. Indirect utility measurements can be measured using the Multi-Attribute Utility Instrument (MAUI) instrument, one of which is the 15D questionnaire that has never been translated and adapted culturally in Indonesian. Objective: Translating and culturally adapting the 15D questionnaire to Indonesian diabetes mellitus patients. Methods: The translation process was carried out through the stages of forward translation, reconciliation, and backward translation involving two qualified translators and three experts in their field. The pilot test stage involved eight respondents consisting of 6 diabetes mellitus patients and two healthy individuals. Results: Problems in the linguistic validation process led to more conformity of word equivalents from the original to the target language. The agreement found was in the area of semantic equivalence, idiomatic equivalence, and experiential equivalence. The problem was resolved by reconciliation during the Focus Group Discussion, which translators and experts in their field attended and discussed with the original author to get equality of meaning in terms of language and culture. The Indonesian version of the 15D questionnaire tested on eight respondents showed results that were easy to understand and straightforward. Conclusion: The Indonesian version of the 15D questionnaire is valid from the linguistic and cultural adaptation stage. Further research is needed relating to the validation and reliability of the questionnaire.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135064829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-02DOI: 10.20473/jfiki.v10i22023.173-183
Ayudia Cipta Khairani, None Tri Wijayanti, None Gunawan Pamudji Widodo
Background: Type 2 diabetes mellitus can cause complications, one of which is diabetic nephropathy. Parameters that indicate damage to the kidneys are the increase in creatinine and albumin levels. One of the traditional medicines used in the treatment of DM is red fruit (Pandanus conoideus Lam). Objective: The purpose of this study was to determine the antihyperglycemic activity and the effect of red fruit oil administration on creatinine levels, microalbumin, and renal histopathology in STZ-NA-induced rats. Methods: This study used 30 male Wistar rats conditioned with type 2 DM with STZ-NA induction. The rats have grouped into 6 groups: group I, the normal control, group II, the negative control, group III, the positive control (pioglitazone 15 mg/kg BW), and groups IV, V, and VI, the red fruit oil respectively 1.35 mL/kg BW,2.7 mL/kg BW, and 5.4 mL/kg BW. Red fruit oil is made in traditional way and prepared for 2 days. Parameters tested in the study include blood glucose levels, creatinine, microalbumin, and kidney histopathology. Data analysis used the ANOVA method followed by Tukey's post hoc test. Results: The results showed that a red fruit oil dose of 5.4 mL/kg BW was an effective dose in reducing blood glucose levels, microalbuminuria, and serum creatinine, and repairing damage to the kidneys of rats. The percent activity of a red fruit oil dose of 5.4 mL/kgBW for blood glucose levels, microalbuminuria and serum creatinine were 84.69%, 76.30%, and 92.20% respectively. Conclusion: Red fruit oil can reduce blood glucose levels, creatinine levels, microalbumin and can repair kidney damage.
{"title":"Antihyperglycemic Activity of Red Fruit Oil (Pandanus conoideus Lam) on Improving Kidney Function in STZ- NA-Induced Nephropathy Rats","authors":"Ayudia Cipta Khairani, None Tri Wijayanti, None Gunawan Pamudji Widodo","doi":"10.20473/jfiki.v10i22023.173-183","DOIUrl":"https://doi.org/10.20473/jfiki.v10i22023.173-183","url":null,"abstract":"Background: Type 2 diabetes mellitus can cause complications, one of which is diabetic nephropathy. Parameters that indicate damage to the kidneys are the increase in creatinine and albumin levels. One of the traditional medicines used in the treatment of DM is red fruit (Pandanus conoideus Lam). Objective: The purpose of this study was to determine the antihyperglycemic activity and the effect of red fruit oil administration on creatinine levels, microalbumin, and renal histopathology in STZ-NA-induced rats. Methods: This study used 30 male Wistar rats conditioned with type 2 DM with STZ-NA induction. The rats have grouped into 6 groups: group I, the normal control, group II, the negative control, group III, the positive control (pioglitazone 15 mg/kg BW), and groups IV, V, and VI, the red fruit oil respectively 1.35 mL/kg BW,2.7 mL/kg BW, and 5.4 mL/kg BW. Red fruit oil is made in traditional way and prepared for 2 days. Parameters tested in the study include blood glucose levels, creatinine, microalbumin, and kidney histopathology. Data analysis used the ANOVA method followed by Tukey's post hoc test. Results: The results showed that a red fruit oil dose of 5.4 mL/kg BW was an effective dose in reducing blood glucose levels, microalbuminuria, and serum creatinine, and repairing damage to the kidneys of rats. The percent activity of a red fruit oil dose of 5.4 mL/kgBW for blood glucose levels, microalbuminuria and serum creatinine were 84.69%, 76.30%, and 92.20% respectively. Conclusion: Red fruit oil can reduce blood glucose levels, creatinine levels, microalbumin and can repair kidney damage.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135064821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Fucoidan is sulfated polysaccharide that has gastroprotector activity, and it is distributed in brown algae cell walls. Currently, there is no method for fucoidan analysis in compendia. Furthermore, analysis of Fucoidan is proven to be challenging due to the lack of chromophores and its high polarity. Objective: To develop the optimal condition of TLC-Densitometry method for fucoidan analysis in Sargassum sp. aqueous extract and to evaluate the stability of Fucoidan as a preliminary study. Methods: Chromatography was performed on Silica gel 60F254 TLC-plate as a stationary phase. The developed plate was stained with H2SO4 10% in absolute ethanol and heated in oven at 105°C for 15 minutes. Optimization is carried out by determining composition of the mobile phase, analytical wavelength, and spotting volume. Stability test of Fucoidan in standard and extract solution at 0, 4, 8, and 24 hours also 0 and 60 minutes after derivatization. Results: The optimal condition which produces a good separation of Fucoidan was achieved by using n-butanol:methanol: water (10:6:10 v/v/v) as a mobile phase, 400 nm as an analytical wavelength, and 1 µl as a spotting volume. Fucoidan was stable after storage until 24 hours. The stained spots were stable until 60 minutes after derivatization. Conclusion: Optimal condition of the TLC-Densitometry method for Fucoidan analysis was selective and can be applied to stability tests in preliminary study. Fucoidan was stable in standard solution and extracted solution until 24 hours after storage at 4°C, and the stained spots were stable until 60 minutes after derivatization.
{"title":"Optimization and Prevalidation of TLC-Densitometry Method for Fucoidan Analysis in Sargassum sp. Aqueous Extract","authors":"Laili Irfanah, None Mochammad Yuwono, None Riesta Primaharinastiti","doi":"10.20473/jfiki.v10i22023.210-216","DOIUrl":"https://doi.org/10.20473/jfiki.v10i22023.210-216","url":null,"abstract":"Background: Fucoidan is sulfated polysaccharide that has gastroprotector activity, and it is distributed in brown algae cell walls. Currently, there is no method for fucoidan analysis in compendia. Furthermore, analysis of Fucoidan is proven to be challenging due to the lack of chromophores and its high polarity. Objective: To develop the optimal condition of TLC-Densitometry method for fucoidan analysis in Sargassum sp. aqueous extract and to evaluate the stability of Fucoidan as a preliminary study. Methods: Chromatography was performed on Silica gel 60F254 TLC-plate as a stationary phase. The developed plate was stained with H2SO4 10% in absolute ethanol and heated in oven at 105°C for 15 minutes. Optimization is carried out by determining composition of the mobile phase, analytical wavelength, and spotting volume. Stability test of Fucoidan in standard and extract solution at 0, 4, 8, and 24 hours also 0 and 60 minutes after derivatization. Results: The optimal condition which produces a good separation of Fucoidan was achieved by using n-butanol:methanol: water (10:6:10 v/v/v) as a mobile phase, 400 nm as an analytical wavelength, and 1 µl as a spotting volume. Fucoidan was stable after storage until 24 hours. The stained spots were stable until 60 minutes after derivatization. Conclusion: Optimal condition of the TLC-Densitometry method for Fucoidan analysis was selective and can be applied to stability tests in preliminary study. Fucoidan was stable in standard solution and extracted solution until 24 hours after storage at 4°C, and the stained spots were stable until 60 minutes after derivatization.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135064823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Puskesmas is a primary healthcare facility that conducts chronic disease management, such as hypertension. The role of pharmacists in team collaboration includes that of managerial and clinical pharmacies. However, doctors and nurses still need to be fully aware of the role of pharmacists, particularly in clinical pharmacies. Objective: This study aimed to determine the perspectives of pharmacists, doctors, and nurses on the collaborative management of hypertension in health centres across the Central Lombok Regency. Methods: observational qualitative method with a maximum variation sampling technique was used. Data saturation was achieved after interviewing 27 participants between April and June 2023. Participants were pharmacists, doctors, and nurses responsible for managing hypertension in the selected primary healthcare centers. Results: Five main themes were identified. The first was a perspective on pharmacists’ managerial and clinical pharmacy roles. Almost all participants agreed that pharmacists played more roles in ensuring the availability of hypertension drugs than clinical pharmacies. Four themes were derived from a conceptual framework related to team readiness to collaborate: cognitive, affective/relational, behavioral, and leadership aspects. In general, doctors and nurses need to be made aware of pharmacists' role in the area of clinical pharmacy; meanwhile, pharmacists need to improve their clinical pharmacy knowledge. Meanwhile, team collaboration has not run optimally because each team member works individually rather than as a team member. Conclusion: Pharmacists need to improve their clinical pharmacy role, be more involved in team collaborations, and be more engaged in team collaborations; efforts are required to prepare for team collaboration.
{"title":"Perspectives of Pharmacists, Doctors, and Nurses on Collaborative Management of Hypertension in Primary Health Centers","authors":"Nia Mariana Siregar, Hanni Prihhastuti Puspitasari, Wahyu Utami","doi":"10.20473/jfiki.v10i22023.246-256","DOIUrl":"https://doi.org/10.20473/jfiki.v10i22023.246-256","url":null,"abstract":"Background: Puskesmas is a primary healthcare facility that conducts chronic disease management, such as hypertension. The role of pharmacists in team collaboration includes that of managerial and clinical pharmacies. However, doctors and nurses still need to be fully aware of the role of pharmacists, particularly in clinical pharmacies. Objective: This study aimed to determine the perspectives of pharmacists, doctors, and nurses on the collaborative management of hypertension in health centres across the Central Lombok Regency. Methods: observational qualitative method with a maximum variation sampling technique was used. Data saturation was achieved after interviewing 27 participants between April and June 2023. Participants were pharmacists, doctors, and nurses responsible for managing hypertension in the selected primary healthcare centers. Results: Five main themes were identified. The first was a perspective on pharmacists’ managerial and clinical pharmacy roles. Almost all participants agreed that pharmacists played more roles in ensuring the availability of hypertension drugs than clinical pharmacies. Four themes were derived from a conceptual framework related to team readiness to collaborate: cognitive, affective/relational, behavioral, and leadership aspects. In general, doctors and nurses need to be made aware of pharmacists' role in the area of clinical pharmacy; meanwhile, pharmacists need to improve their clinical pharmacy knowledge. Meanwhile, team collaboration has not run optimally because each team member works individually rather than as a team member. Conclusion: Pharmacists need to improve their clinical pharmacy role, be more involved in team collaborations, and be more engaged in team collaborations; efforts are required to prepare for team collaboration.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135064820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-02DOI: 10.20473/jfiki.v10i22023.257-265
Alma Nuril Aliyah, Pingkan Aprilia, Anak Agung Sagung Dyah Pramesti, Tarisya Dinda Saraya, Galuh Laksatrisna Pide, I Nengah Budi Sumartha, Luke Wongso, None Samirah, Mahardian Rahmadi, None Muhammad Zaki Bin Ramli, Chrismawan Ardianto
Background: CIPN (Chemotherapy-induced Peripheral Neuropathy) primarily affects the sensory system and is accompanied by pain, autonomic dysfunction, and motor impairments. Alterations of intracellular second messengers at the supraspinal level in CIPN needed to be explored more. In addition, there is a lack of evidence regarding implications for the supraspinal area through the propagation of pain via the ascending pathway. Objective: In this study, we evaluated the effect of fenofibrate as a PPARα agonist in suppressing the development of CIPN. Methods: Twenty-four mice were distributed to the normal control group, neuropathy group, and neuropathy with the treatment of fenofibrate 75 and 150 mg/kg groups, resulting in 6 animals per group. Oxaliplatin was injected on days 0, 2, 4, and 6. The hot plate test was performed before the oxaliplatin administration and then continued on the 7th, 14th, and 21st days. Thalamus tissues were collected to measure the TRPA1 mRNA expression using qPCR. Results: Fenofibrate 75 mg/kg co-treatment with oxaliplatin tended to prevent the enhancement of oxaliplatin-induced thermal hyperalgesia in hind-paw withdrawal and rubbing responses. Furthermore, fenofibrate 75 and 150 mg/kg co-treatment with oxaliplatin significantly reduced the relative TRPA1 mRNA expression but did not modulate the relative BDNF mRNA expression in the thalamus. Conclusion: PPARα agonist has a potential effect in suppressing the development of CIPN. However, given the various perspectives on the role of neurotrophins in CIPN, additional non-clinical investigations, are needed to provide more insight into other mechanisms of CIPN and the role of PPAR agonists.
{"title":"Effect of Fenofibrate as PPARα Agonist in Suppressing the Development of Oxaliplatin-Induced Peripheral Neuropathy via TRPA1 Modulation","authors":"Alma Nuril Aliyah, Pingkan Aprilia, Anak Agung Sagung Dyah Pramesti, Tarisya Dinda Saraya, Galuh Laksatrisna Pide, I Nengah Budi Sumartha, Luke Wongso, None Samirah, Mahardian Rahmadi, None Muhammad Zaki Bin Ramli, Chrismawan Ardianto","doi":"10.20473/jfiki.v10i22023.257-265","DOIUrl":"https://doi.org/10.20473/jfiki.v10i22023.257-265","url":null,"abstract":"Background: CIPN (Chemotherapy-induced Peripheral Neuropathy) primarily affects the sensory system and is accompanied by pain, autonomic dysfunction, and motor impairments. Alterations of intracellular second messengers at the supraspinal level in CIPN needed to be explored more. In addition, there is a lack of evidence regarding implications for the supraspinal area through the propagation of pain via the ascending pathway. Objective: In this study, we evaluated the effect of fenofibrate as a PPARα agonist in suppressing the development of CIPN. Methods: Twenty-four mice were distributed to the normal control group, neuropathy group, and neuropathy with the treatment of fenofibrate 75 and 150 mg/kg groups, resulting in 6 animals per group. Oxaliplatin was injected on days 0, 2, 4, and 6. The hot plate test was performed before the oxaliplatin administration and then continued on the 7th, 14th, and 21st days. Thalamus tissues were collected to measure the TRPA1 mRNA expression using qPCR. Results: Fenofibrate 75 mg/kg co-treatment with oxaliplatin tended to prevent the enhancement of oxaliplatin-induced thermal hyperalgesia in hind-paw withdrawal and rubbing responses. Furthermore, fenofibrate 75 and 150 mg/kg co-treatment with oxaliplatin significantly reduced the relative TRPA1 mRNA expression but did not modulate the relative BDNF mRNA expression in the thalamus. Conclusion: PPARα agonist has a potential effect in suppressing the development of CIPN. However, given the various perspectives on the role of neurotrophins in CIPN, additional non-clinical investigations, are needed to provide more insight into other mechanisms of CIPN and the role of PPAR agonists.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135064822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-02DOI: 10.20473/jfiki.v10i22023.151-161
Dioni fadia Zatalini, Esti Hendradi, None Philip Drake, Retno Sari
Background: Wound is a condition where there is damage or disruption to the anatomical structure and function of the skin. Wounds that are not treated properly can lead to infection. As wound dressings, film dressings have many advantages such as being elastic, flexible, transparent, and can adapt to the wound shape. Film’s characteristics of are affected by the plasticizer and the polymer. Combination of chitosan and polyvinyl alcohol (PVA) is able to improve the mechanical properties of the film such as its swelling capacity, tensile strength, and elongation at break. Objective: This study aims to determine the effects of chitosan and PVA in various concentrations on the physical characteristics and mechanical properties of the film. Methods: Film was prepared by solvent casting method, using chitosan and alginate in various concentrations of 0% to 1.5%, 1.5% Aloe vera, and 6% propylene glycol. Films’ characteristics and mechanical properties were evaluated, such as swelling index, tensile strength, elongation at break, and Young’s modulus. Results: The result showed that chitosan and PVA polymers had a significant effect on the swelling index, tensile strength, elongation at break, and Young’s modulus. The effect of chitosan and PVA combination on the swelling index, tensile strength, and elongation at break is due to the hydrogen bonding between the hydroxyl group of PVA and the amine group of chitosan. Conclusion: The combination of chitosan and polyvinyl alcohol influenced the film's physical and mechanical properties. Film with chitosan and polyvinyl alcohol ratio of 1.5%:1.5% have best characteristics compared to others.
{"title":"The Effect of Chitosan and Polyvinyl Alcohol Combination on Physical Characteristics and Mechanical Properties of Chitosan-PVA-Aloe vera Film","authors":"Dioni fadia Zatalini, Esti Hendradi, None Philip Drake, Retno Sari","doi":"10.20473/jfiki.v10i22023.151-161","DOIUrl":"https://doi.org/10.20473/jfiki.v10i22023.151-161","url":null,"abstract":"Background: Wound is a condition where there is damage or disruption to the anatomical structure and function of the skin. Wounds that are not treated properly can lead to infection. As wound dressings, film dressings have many advantages such as being elastic, flexible, transparent, and can adapt to the wound shape. Film’s characteristics of are affected by the plasticizer and the polymer. Combination of chitosan and polyvinyl alcohol (PVA) is able to improve the mechanical properties of the film such as its swelling capacity, tensile strength, and elongation at break. Objective: This study aims to determine the effects of chitosan and PVA in various concentrations on the physical characteristics and mechanical properties of the film. Methods: Film was prepared by solvent casting method, using chitosan and alginate in various concentrations of 0% to 1.5%, 1.5% Aloe vera, and 6% propylene glycol. Films’ characteristics and mechanical properties were evaluated, such as swelling index, tensile strength, elongation at break, and Young’s modulus. Results: The result showed that chitosan and PVA polymers had a significant effect on the swelling index, tensile strength, elongation at break, and Young’s modulus. The effect of chitosan and PVA combination on the swelling index, tensile strength, and elongation at break is due to the hydrogen bonding between the hydroxyl group of PVA and the amine group of chitosan. Conclusion: The combination of chitosan and polyvinyl alcohol influenced the film's physical and mechanical properties. Film with chitosan and polyvinyl alcohol ratio of 1.5%:1.5% have best characteristics compared to others.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135064828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-12DOI: 10.35814/jifi.v21i1.1397
Zainur Rahman Hakim, Ratna Djamil, Novi Yantih, Ocitania Sanasih, Benny Iskandar
This study examined the tyrosinase inhibitory potential of bengkoang peel standardised ethanolic extract and ethyl acetate fractions. 450 g of bengkoang peel powder (Pachyrhizus erosus L.) was macerated with 96% ethanol for 2 x 24 h, filtered, and concentrated till viscous. Extract standardisation uses specific and non-specific parameters. Microplate colorimetric tyrosinase inhibition assays were performed. The result of plant determination identified the bengkoang peel as Pachyrhizus erosus (L.) Urb. The organoleptic test revealed a brownish-yellow viscous extract, fresh smell, and bitter taste. Ethanol-soluble extract was 47.60% and water-soluble 22.42%. Bengkoang peel ethanolic extract includes alkaloids, flavonoids, saponins, tannins, and steroids. The non-specific parameters showed loss of drying (9.71%), water content (7.28%), total ash (9.91%), acid-insoluble ash (3.68%), no residual solvent content, lead content (-0.2324±0.1729), cadmium content (-0.0841±0.3418), TPC of 1 x 101 CFU/g, and TYMC of 2.26 x 102 CFU/g. The ethanol extract (103.9263) and ethyl acetate fraction (81.8606) IC50 values of bengkoang peel decrease tyrosinase enzyme activity compared to control (25.1235 ppm). These results show that the ethanol extract met both particular and non-specific quality standards. Bengkoang peel ethyl acetate inhibited tyrosinase.
{"title":"Potential of The Tyrosinase Enzyme Inhibition by Standardized Ethanol Extract And Ethyl Acetate Fraction of Bengkoang Peel (Pachyrhizus erosus L.)","authors":"Zainur Rahman Hakim, Ratna Djamil, Novi Yantih, Ocitania Sanasih, Benny Iskandar","doi":"10.35814/jifi.v21i1.1397","DOIUrl":"https://doi.org/10.35814/jifi.v21i1.1397","url":null,"abstract":"This study examined the tyrosinase inhibitory potential of bengkoang peel standardised ethanolic extract and ethyl acetate fractions. 450 g of bengkoang peel powder (Pachyrhizus erosus L.) was macerated with 96% ethanol for 2 x 24 h, filtered, and concentrated till viscous. Extract standardisation uses specific and non-specific parameters. Microplate colorimetric tyrosinase inhibition assays were performed. The result of plant determination identified the bengkoang peel as Pachyrhizus erosus (L.) Urb. The organoleptic test revealed a brownish-yellow viscous extract, fresh smell, and bitter taste. Ethanol-soluble extract was 47.60% and water-soluble 22.42%. Bengkoang peel ethanolic extract includes alkaloids, flavonoids, saponins, tannins, and steroids. The non-specific parameters showed loss of drying (9.71%), water content (7.28%), total ash (9.91%), acid-insoluble ash (3.68%), no residual solvent content, lead content (-0.2324±0.1729), cadmium content (-0.0841±0.3418), TPC of 1 x 101 CFU/g, and TYMC of 2.26 x 102 CFU/g. The ethanol extract (103.9263) and ethyl acetate fraction (81.8606) IC50 values of bengkoang peel decrease tyrosinase enzyme activity compared to control (25.1235 ppm). These results show that the ethanol extract met both particular and non-specific quality standards. Bengkoang peel ethyl acetate inhibited tyrosinase.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81614337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-11DOI: 10.35814/jifi.v21i1.1401
D. Rahmat, Safira Nafisa, Catharina Maria Odilia
Black Sea cucumbers (Holothuria atra) are a potential source of active metabolites such as flavonoids widely used for nutraceutical, pharmaceutical, and cosmeceutical products. The type of nanoparticle that is often used in cosmetics and skin products is Solid Lipid Nanoparticles (SLN). In this study, the SLN of black sea cucumber extract will be prepared in the form of a peel-off gel mask as an antioxidant. Black sea cucumbers were extracted using the maceration method, while SLN preparation used high-shear homogenization and freeze-drying methods. Crude extract and SLN were formulated into peel-off gels and then evaluated, including organoleptic, homogeneity, viscosity, pH, spreadability, drying time, tensile strength test, and antioxidant activity using the DPPH method. The IC50 values of blank (F1), peel-off gel extract (F2), and nanoparticle peel-off gel extract (F3) were each 600.8727 ± 0.43; 580.7849±0.7; and 592.76±0.55 µg/mL. In conclusion, the SLN method can be applied to the formulation of black sea cucumber extract nanoparticles. However, peel-off gel masks from the crude and nanoparticle extracts have very weak antioxidant activity.
{"title":"Application of Solid Lipid Nanoparticles Method in Black Sea Cucumber (Holothuria atra) Extract Peel-off Gel Mask and Antioxidant Activity Tests","authors":"D. Rahmat, Safira Nafisa, Catharina Maria Odilia","doi":"10.35814/jifi.v21i1.1401","DOIUrl":"https://doi.org/10.35814/jifi.v21i1.1401","url":null,"abstract":"Black Sea cucumbers (Holothuria atra) are a potential source of active metabolites such as flavonoids widely used for nutraceutical, pharmaceutical, and cosmeceutical products. The type of nanoparticle that is often used in cosmetics and skin products is Solid Lipid Nanoparticles (SLN). In this study, the SLN of black sea cucumber extract will be prepared in the form of a peel-off gel mask as an antioxidant. Black sea cucumbers were extracted using the maceration method, while SLN preparation used high-shear homogenization and freeze-drying methods. Crude extract and SLN were formulated into peel-off gels and then evaluated, including organoleptic, homogeneity, viscosity, pH, spreadability, drying time, tensile strength test, and antioxidant activity using the DPPH method. The IC50 values of blank (F1), peel-off gel extract (F2), and nanoparticle peel-off gel extract (F3) were each 600.8727 ± 0.43; 580.7849±0.7; and 592.76±0.55 µg/mL. In conclusion, the SLN method can be applied to the formulation of black sea cucumber extract nanoparticles. However, peel-off gel masks from the crude and nanoparticle extracts have very weak antioxidant activity.","PeriodicalId":17684,"journal":{"name":"JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78353417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：