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The Effect of Lycopene Addition on the Semen Quality of Saanen Goats Stored at 5°C for 24 hours 番茄红素添加量对5℃保存24 h的沙嫩山羊精液质量的影响
Pub Date : 2023-03-21 DOI: 10.23960/jipt.v11i1.p34-40
S. Bintara, D. T. Widayati, W. Asmarawati, R. N. Aji
In order to increase the livestock population, a good reproductive process was needed. Lycopene which belongs to carotenoid group has a dark yellow pigment. This study aimed to determine the effect of adding lycopene on the semen quality of Saanen goats. The research design used 4 treatments, P0 (egg yolk tris diluent) as control), P1 (egg yolk tris diluent + 2% lycopene), P2 (egg yolk tris diluent + 4% lycopene), and P3 (yolk tris diluent). egg + 6% lycopene). The quality of semen was observed after 24 hours of storage at 5℃. Macroscopic data were analyzed descreptively and the microscopic one was analyzed using a completely randomized design (CRD). The results showed that the addition of lycopene in egg yolk tris diluent had a significant (P<0.05) effect on the semen quality of Saanen goats. The results showed that the motility of spermatozoa in each treatment group (P0, P1, P2, and P3) was 60.1 ± 5.7%, 65.2 ± 4.3%, 62.4 ± 5.2%, and 60.6 ± 4.7%, respectively, viability were 65.3 ± 4.8, 73.5 ± 4.2, 70.4 ± 3.8, and 66.1 ± 5.1, respectively, abnormality were 17 ± 1.8%, 16 ± 1.8%, 18 ± 1.9%, and 16 ± 1.7%, respectively, and membrane integrity were 63 ± 3.3%, 70 ± 3.6 %, 67 ± 3.2%, and 62 ± 2.9%, respectively. Based on the results, it could be concluded that the lycopene addition improved the semen quality of Saanen goats compared to controls.
为了增加牲畜数量,需要一个良好的繁殖过程。番茄红素属于类胡萝卜素类,具有暗黄色色素。本研究旨在测定添加番茄红素对萨能山羊精液质量的影响。研究设计使用了4种处理,P0(蛋黄三稀释剂)作为对照,P1(蛋黄三稀释液+2%番茄红素),P2(蛋黄三浓缩液+4%番茄红素)和P3(蛋黄三混合稀释剂)。鸡蛋+6%番茄红素)。精液在5℃下保存24小时后,观察精液质量。宏观数据进行描述性分析,微观数据采用完全随机设计(CRD)进行分析。结果表明,蛋黄三稀释液中添加番茄红素对萨能山羊精液质量有显著影响(P<0.05)。结果显示,各处理组(P0、P1、P2和P3)精子活力分别为60.1±5.7%、65.2±4.3%、62.4±5.2%和60.6±4.7%,活力分别为65.3±4.8、73.5±4.2、70.4±3.8和66.1±5.1,异常分别为17±1.8%、16±1.8%、18±1.9%和16±1.7%,膜完整性分别为63±3.3%、70±3.6%,分别为67±3.2%和62±2.9%。根据研究结果,可以得出结论,与对照组相比,番茄红素的添加改善了萨能山羊的精液质量。
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引用次数: 0
Inulin Extraction from Different Types of Tubers in Magelang District 马哲朗地区不同类型块茎菊粉的提取
Pub Date : 2023-03-20 DOI: 10.23960/jipt.v11i1.p1-12
L. Hartati, M. H. Septian, Noor Alif Fitria, R. W. Idayanti, M. Sihite
This study aimed to determine the levels and characteristics of inulin contained in various types of tubers in Magelang district. The samples used were honey sweet potato (Ipomoea batatas L.), purple sweet potato (Ipomoea batatas L.), white sweet potato (Ipomoea batatas L.), gadung (Dioscorea hispida), yam (Dioscorea alata L.), canna (Canna edulis Ker.), and arrowroot (Maranta arundinacea). The variables observed were yield, inulin content, inulin production, water content of the yield, yield solubility, and the degree of acidity (pH) of the yield. The research results obtained from 7 tubers, the highest yield was arrowroot tubers (0.155%). Honey sweet potato gave the lowest yield (0.035%), with the highest inulin content (6.472%), the highest water content (14.385%), and the highest solubility (0.685%). Arrowroot tubers gave the highest yield but the lowest inulin content. The highest inulin production is found in purple sweet potato. The degree of acidity (pH) of the tuber extract was in the range of 4.97-5.96. The conclusion of this study is that different types of tubers produce different content and production of inulin.
本研究旨在测定马格朗地区不同类型块茎中菊粉的含量和特征。所用样品为蜜甘薯(Ipomoea batatas L.)、紫甘薯(Ipomoea batatas L.)、白甘薯(Ipomoea batatas L.)、薯蓣(Dioscorea hispida)、山药(Dioscorea alata L.)、美人蕉(canna edulis Ker.)和竹菜(Maranta arundinacea)。考察了产率、菊粉含量、菊粉产量、产率的含水量、产率的溶解度和产率的酸度(pH)。研究结果表明,7种块茎产量最高的是竹芋块茎(0.155%)。甘薯产率最低(0.035%),菊粉含量最高(6.472%),水分含量最高(14.385%),溶解度最高(0.685%)。根块茎产量最高,但菊粉含量最低。紫甘薯的菊粉产量最高。块茎提取物的酸度(pH)在4.97 ~ 5.96之间。本研究的结论是,不同类型的块茎产生不同的菊粉含量和产量。
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引用次数: 0
Evaluation of Motility, Viability and Abnormality of Boar Spermatozoa in Various Modified Extenders 猪精子在各种改良增种器中的活力、活力和异常性评价
Pub Date : 2023-03-20 DOI: 10.23960/jipt.v11i1.p13-33
Hermilinda Parera, V. Lenda
The purpose of this study was to conduct an assessment on the quality of pig semen spermatozoa during storage and determine which diluent material should be used and how long pig semen can be stored. Freshly ejaculated boar semen was obtained from a two-years-old Duroc male and was diluted with Beltsville Thawing Solution (BTS) and Tris Citrate Fructose (TCF) supplemented with 5% egg yolk (KT) and extract of Borassus flabellifer Linn mesocarp (EM) 0.01% and stored at 13°C for up to 4 days. An experimental study with a completely randomized design with four groups and five repeats was applied. Parameters observed were motility, viability and abnormality of spermatozoa. Sperm quality was evaluated by examining motility and abnormality using a phase-contrast microscope with a magnification of 100X, and viability by eosin nigrosine staining. Parametric data were analyzed with ANOVA followed by Duncan test.  The results showed that the supplementation of 5% egg yolk in the BTS diluent-based treatment group showed a higher percentage of motility (P<0.05) than without egg yolk, while the TCF-based diluent group showed no differences (P<0.05). Modified-Tris Citrate Fructose diluent supplemented with 5% egg yolk and 0.01% mesocarp extract was able to maintain a significantly higher percentage of viability (67.50±2.0) (P<0.05) than other groups, and had a percentage of abnormalities (9.75±0.957), significantly lower (P<0.05) than other groups. It was concluded that the modification of diluent TCF with 5% egg yolk and mesocarp extract 0.01% provide the best results in maintaining sperm quality with the highest percentage of sperm motility and viability, and the lowest percentage of sperm abnormality compared to the other three diluents. Likewise, all groups of modified-BTS and TCF diluent supplemented with 5% egg yolk and extract of Borassus flabellifer Linn mesocarp in this study were able to maintain motility >40%, viability >45% and abnormalities <20% so they were appropriate for using in the AI procedure.
本研究的目的是对猪精液精子在储存过程中的质量进行评估,确定应使用哪种稀释剂以及猪精液的储存时间。选取2岁杜罗克公猪新鲜射精精液,用贝尔茨维尔解冻液(BTS)和柠檬酸三酯果糖(TCF)稀释,并添加5%蛋黄(KT)和0.01%牛膝草中果皮(EM)提取物,在13℃下保存4 d。试验采用完全随机设计,四组五重复。观察精子的活力、活力和异常情况。通过100倍的相衬显微镜检查精子的运动和异常,通过伊红黑氨酸染色来评估精子的质量。参数资料采用方差分析及Duncan检验。结果表明,以BTS稀释剂为基础的处理组,添加5%的蛋黄,其活力率(P40%)、活力率(>)和异常率(<20%)均较高,适合用于人工智能手术。
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引用次数: 0
Identification and Pathogenicity of Escherichia coli from Cloacal Swabs 粪拭子大肠杆菌鉴定及致病性研究
Pub Date : 2023-01-30 DOI: 10.23960/jipt.v10i3.p323-332
Arie Khoiriyah, S. Sumardi, H. Busman
Colibacillosisis is an infectious disease in poultry caused by pathogenic Escherichia coli (E. coli) as primary or secondary agents. This study were aim to identify and test the pathogenicity ofE. coli isolated from chicken cloacal swabs. The samples used in this study were E. coli isolated from chicken cloacal swabs as many as 42 samples. The first stage in this research was subculturing E.coli in Eosin Methylene Blue Agar (EMBA) medium, then identified by Gram stain and biochemistry with Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Simmons Citrate Agar (SCA) and Methyl Red Voges-Proskauer (MRVP). Pathogenicity of E. coli was tested by culturing in Blood Agar to see the results of hemolysin production and on Sorbitol-MacConkey Agar (SMAC) to see the ability of Sorbitol fermentation. The results obtained were 12 isolates was pathogenic E. coli looked from the results of the pathogenicity test in the Blood Agar and SMAC Agar.
大肠杆菌病是由致病性大肠杆菌(E. coli)作为主要或次要病原体引起的家禽传染病。本研究旨在鉴定和检测大肠杆菌的致病性。从鸡粪拭子中分离出大肠杆菌。本研究中使用的样本是从鸡粪拭子中分离的大肠杆菌多达42个样本。本研究的第一阶段是在伊红亚甲基蓝琼脂(EMBA)培养基中继代培养大肠杆菌,然后用三糖铁琼脂(TSIA)、硫吲哚琼脂(SIM)、西蒙斯柠檬酸琼脂(SCA)和甲基红Voges-Proskauer (MRVP)进行革兰氏染色和生化鉴定。在血琼脂培养基上检测大肠杆菌的致病性,观察溶血素的产生情况;在山梨糖醇-麦康基琼脂培养基上检测山梨糖醇的发酵能力。从血琼脂和SMAC琼脂的致病性试验结果来看,12株分离株为致病性大肠杆菌。
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引用次数: 0
Improvement of Water Hyacinth (Eichhornia crassipes) Quality as Animal Feed through Fermentation Using Rumen Microbes with the Addition of Molasses 添加糖蜜及瘤胃微生物发酵提高水葫芦饲料品质
Pub Date : 2023-01-09 DOI: 10.23960/jipt.v10i3.p313-322
Desi Susanti, Suci Rahmi
One of the potential alternative feed ingredients as animal feed is water hyacinth. However, the low quality and palatability cause water hyacinth not being liked by ruminants. One way to improve the quality of water hyacinth is using controlled technology involving rumen microbes. The purpose of the study was to determine the level of addition of molasses and fermentation time in improving the quality of water hyacinth using rumen microbes through the test parameters for crude protein and crude fiber content. This study used a completely randomized design (CRD) with factorial patterns with molasses levels, namely M1 = 5%, M2 = 10%, M3 = 15%, and fermentation time consisting of T1 = 7 days, T2 = 10 days, T3 = 13 days . Each experimental design was repeated 3 times to form 27 experimental units. Data were analyzed using variance, if there was a significant treatment effect (P<0.05), followed by the HSD test on the average treatment. The results showed that the highest crude protein content was 14.23% in the M2T3 treatment (10% molasses level with 13 days of fermentation time) (P<0.05) while the lowest crude fiber was 14.55% in the M3T2 treatment (15% molasses level with a dangerous time) 10 days). This treatment was significantly different (P<0.05) from the other treatments. The results of the study concluded that the addition of 10% molasses for 13 days of brewing time could increase the crude protein content in water hyacinth fermented using rumen-filled microbes.and the lowest decrease in crude fiber was at 15% molasses level with 10 days of fermentation.
水葫芦是一种潜在的动物饲料替代品。然而,水葫芦的质量和适口性不高,导致反刍动物不喜欢水葫芦。提高水葫芦质量的一种方法是使用涉及瘤胃微生物的控制技术。本试验旨在通过测定水葫芦粗蛋白质和粗纤维含量,确定糖蜜添加水平和发酵时间对瘤胃微生物提高水葫芦品质的影响。本研究采用完全随机设计(CRD),采用糖蜜水平的析因模式,即M1 = 5%, M2 = 10%, M3 = 15%,发酵时间T1 = 7天,T2 = 10天,T3 = 13天。每个试验设计重复3次,共27个试验单元。若有显著治疗效果(P<0.05),采用方差分析,对平均治疗进行HSD检验。结果表明:M2T3处理(10%糖蜜水平,发酵时间13 d)粗蛋白质含量最高,为14.23% (P<0.05); M3T2处理(15%糖蜜水平,危险时间10 d)粗纤维含量最低,为14.55%。该处理与其他处理差异有统计学意义(P<0.05)。结果表明,添加10%糖蜜,发酵时间为13 d,可提高瘤胃微生物发酵水葫芦的粗蛋白质含量。发酵10 d时,粗纤维含量下降幅度最小,为15%糖蜜水平。
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引用次数: 0
Physical, Chemical Quality and Constituent of Amoniated Cassava Peel with Various Levels of Urea Addition 不同尿素添加量对木薯皮理化性质及组成的影响
Pub Date : 2023-01-08 DOI: 10.23960/jipt.v10i3.p300-312
F. Fathul, E. Erwanto, A. K. Wijaya, A. Dakhlan, F. T. Farda, Etha Azizah Hasiib
Cassava peel is a waste from the cassava plant which has the potential to be used as ruminant animal feed. Processing of cassava skin with ammonia needs to be done to improve the quality of cassava skin. The research was conducted to determine the physical and chemical quality of cassava peels after amoniation. The study was analyzed using a completely randomized design (CRD) and the significant results were further tested using Duncan's multiple range test (DMRT). The treatment was T1 (cassava peels + 0kg urea); T2 (cassava peels + 0.02kg urea) ; T3 (Cassava peels + 0.04kg urea). Each treatment consisted of 5 repetitions. The organoleptic test was carried out with 30 test participants. The physical tests observed were color, texture and aroma. Chemical quality was determined through proximate analysis and Scanning Electron Microscope-Energy Dispersive X-Ray (SEM-EDX). Variables in the chemical quality test included dry matter, ash, crude protein, ether extract and crude fiber of cassava peels. The results showed that ammoniation of cassava peels affected the physical and chemical qualities of cassava peels (P<0.05). Ammoniation of cassava peel increases protein content up to 23.01% (T3) and reduces crude fiber up to 7.92% (T2). Increasing the level of urea can increase the process of degradation of starch structure in cassava peels.
木薯皮是木薯植物的废弃物,有可能用作反刍动物的饲料。需要用氨水处理木薯皮以提高木薯皮的质量。本研究旨在测定氨化后木薯皮的理化性质。使用完全随机设计(CRD)对该研究进行分析,并使用邓肯多范围检验(DMRT)对显著结果进行进一步检验。处理为T1(木薯皮+0kg尿素);T2(木薯皮+0.02kg尿素);T3(木薯皮+0.04kg尿素)。每次治疗包括5次重复。感官测试由30名测试参与者进行。观察到的物理测试是颜色、质地和香气。化学性质通过近似分析和扫描电子显微镜能量分散X射线(SEM-EDX)测定。化学质量测试的变量包括木薯皮的干物质、灰分、粗蛋白质、醚提取物和粗纤维。结果表明,氨化处理对木薯皮的理化性质有影响(P<0.05),氨化可使木薯皮蛋白质含量提高23.01%(T3),粗纤维含量降低7.92%(T2)。提高尿素水平可以增加木薯皮淀粉结构的降解过程。
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引用次数: 0
Utilization of Duck Feet Gelatin with the Additional Glycerol as A Plasticizer on the Physical Properties of Edible Film 添加甘油增塑剂的鸭脚明胶对食用薄膜物理性能的影响
Pub Date : 2023-01-01 DOI: 10.23960/jipt.v10i3.p289-299
Hana Aulia Andiati, Jajang Gumilar, E. Wulandari
The packaging that is widely used is plastic packaging, but this material has a weakness that is difficult to decompose and isn’t biodegradable, therefore edible film can be an alternative primary packaging for food products. The material used consists of gelatine made from duck feet with the addition of various concentrations of glycerol.  Duck feet is an underutilized portion of the animal's anatomy, yet they contain collagen, which has the potential to be the raw material for gelatine and edible films. The study was undertaken utilizing duck feet as a source of raw material, which was subsequently processed into gelatine for use in the production of edible films using different glycerol treatments. The treatments comprised of glycerol concentrations of P1 = 10%, P2 = 20%, P3 = 30%, P4 = 40%, P5 = 50%, and P6 = 60%. The findings indicated that the application of glycerol significantly affected the films' solubility, water absorption, and tensile strength (P<0.05), but didn’t significantly affect the elongation of edible films. (P>0.05). The optimal concentration of glycerol for edible film in this study was obtained by using 20% glycerol, with a physical characteristic value of 40.25% solubility, 24.23% water absorption, tensile strength is 0.57 MPa, and elongation is 90%.
广泛使用的包装是塑料包装,但这种材料有一个弱点,即难以分解且不可生物降解,因此可食用薄膜可以作为食品的替代初级包装。所用的材料由添加了不同浓度甘油的鸭掌制成的明胶组成。鸭掌是动物解剖学中一个未被充分利用的部分,但它们含有胶原蛋白,有可能成为明胶和可食用薄膜的原料。这项研究是以鸭掌为原料进行的,随后将其加工成明胶,用于使用不同的甘油处理生产可食用薄膜。处理包括P1=10%、P2=20%、P3=30%、P4=40%、P5=50%和P6=60%的甘油浓度。结果表明,甘油的用量对涂膜的溶解性、吸水性和拉伸强度有显著影响(P0.05)。本研究采用20%的甘油作为食用膜的最佳甘油浓度,其物理特性值为40.25%的溶解性,24.23%的吸水性,拉伸强度为0.57MPa,伸长率为90%。
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引用次数: 0
Performance of Female Thin Tail Sheep Fed Ration Containing Fermented Shrimp Waste by EM-4 EM-4对含发酵虾渣饲粮母细尾羊生产性能的影响
Pub Date : 2023-01-01 DOI: 10.23960/jipt.v10i3.p277-288
Ibnul Abror Musthofa, Budi Ayuningsih, I. Hernaman
The use of shrimp waste as ruminant feed is constrained by the presence of chitin which is difficult to digest. This study aimed to determine the use of shrimp waste fermented with EM-4 as additional feed for female Thin Tailed sheep. A total of 18 female Thin-Tailed sheep with a body weight of 10.35±0.88 kg were allocated to 3 ration treatments containing fermented shrimp waste with EM-4, namely 5%, 7.5% and 10%. The sheep were kept for 90 days at CV. Bungah Tani Djaya's Livestock to measure its performance. The results showed that the use of fermented shrimp waste up to 10% did not interfere with the performance of femaleThin-Tailed sheep with the average dry matter consumption, crude protein consumption, total digestible nutrient (TDN) consumption, daily body weight gain, and feed conversion were 340,29 g/head/day, 31.68 g/head/day, 195.63 g/head/day, 61.80 g/head/day, and 6.18 respectively. The results of the study concluded that the use of shrimp waste fermented with EM-4 up to 10% can be used as additional feed for female Thin-Tailed sheep.
虾粪便作为反刍动物饲料的使用受到难以消化的甲壳质的限制。本研究旨在确定用EM-4发酵的虾渣作为雌性细尾羊的附加饲料的用途。将18只体重为10.35±0.88kg的雌性细尾羊分为3组,分别为5%、7.5%和10%。这些羊在CV Bungah Tani Djaya的牲畜饲养了90天,以衡量其性能。结果表明,发酵虾渣的使用量高达10%对肥瘦尾羊的生产性能没有影响,平均干物质消耗量、粗蛋白消耗量、总可消化营养素消耗量、日增重量和饲料转化率分别为340,29g/头/天、31.68g/头/日、195.63g/头/天,61.80g/头/天和6.18。研究结果表明,使用高达10%的EM-4发酵虾渣可以作为雌性瘦尾羊的附加饲料。
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引用次数: 0
Anaerobic Fermentation of Kepok Banana (Musa Paradisiaca) Peels with Different Levels of Tapioca for Reducing Their Fiber Components 不同浓度木薯粉对枇杷果皮厌氧发酵降低纤维成分的影响
Pub Date : 2022-12-31 DOI: 10.23960/jipt.v10i3.p254-263
T. N. I. Koni, Lodofikus Tori, T. A. Y. Foenay
Banana peel is restricted to use as feedstuffs because it has a high crude fiber content. The reduction of crude fiber can be done by anaerobic fermentation such as silage making. Soluble carbohydrates are needed as an energy source for microorganisms in ensilage process. Tapioca is one of soluble carbohydrates that can be used to improve silage quality. The objective of this study was to evaluate effect of tapioca levels on the fiber fraction of kepok banana peel silage (Musa paradisiaca). A completely randomized design was used in this study, with 4 treatments and 5 replications, namely T0: without tapioca, T5: 5% tapioca, T10: 10% tapioca, T15: 15% tapioca. Variables observed were the content of NDF (%), ADF (%), hemicellulose (%), cellulose (%), and lignin (%).Collected data was  analyzed by analysis of variance and if it had a significant effect, it was continued with Duncan's multiple range test. The results of analysis of variance showed that the level of tapioca had a highly significant effect (P<0.01) on NDF, ADF, hemicellulose, cellulose, and lignin. DMRT showed that the lowest NDF content was at T15 with an average of 48.06%, the lowest ADF was found at T15 with an average of 20.20%, the lowest hemicellulose was found at T15 with an average of 27.46%, the lowest cellulose was found at T10 with an average of 7.98% and the lowest lignin against T15 with an average of 12.09%. In conclusions, 1) Different levels of tapioca was can reduce ADF, NDF, hemicellulose, cellulose, and lignin in kepok banana peel silage, 2) The use of 10% tapioca reduced the highest kepok banana peel silage fiber fraction.
香蕉皮由于其粗纤维含量高而被限制用作饲料。粗纤维的减少可以通过厌氧发酵如青贮饲料的制作来实现。在青贮过程中,微生物需要可溶性碳水化合物作为能量来源。木薯淀粉是一种可溶性碳水化合物,可用于提高青贮饲料的质量。本研究的目的是评估木薯淀粉水平对kepok香蕉皮青贮饲料(Musa paradisiaca)纤维含量的影响。本研究采用完全随机设计,4个处理和5个重复,即T0:不含木薯,T5:5%木薯,T10:10%木薯,T15:15%木薯。观察到的变量是NDF(%)、ADF(%。方差分析结果表明,木薯淀粉对NDF、ADF、半纤维素、纤维素和木质素的影响非常显著(P<0.01)。DMRT显示,NDF含量最低的是T15,平均48.06%,ADF最低的是T15,平均20.20%,最低的半纤维素是T15的平均27.46%,最低的纤维素是T10的平均7.98%,最低的木质素是针对T15的,平均12.09%,kepok香蕉皮青贮饲料中的NDF、半纤维素、纤维素和木质素,2)使用10%的木薯粉降低了kepok蕉皮青贮饲料的最高纤维分数。
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引用次数: 0
Identification of Single Nucleotide Polymorphism (SNP) in Early Exon 10 of Follicle Stimulating Hormone Receptor (FSHR) Gene in Pesisir Cattle Pessir牛卵泡刺激激素受体(FSHR)基因早期外显子10单核苷酸多态性的鉴定
Pub Date : 2022-12-31 DOI: 10.23960/jipt.v10i3.p264-276
T. Afriani, E. Purwati, J. Hellyward, Jaswandi Jaswandi, M. Mundana, A. Farhana, A. Rastosari
The purpose of this study was to identify genetic diversity in the early exon 10 of the FSHR gene or follicle stimulating hormone receptor in Pesisir cattle. The blood samples used were 70 blood samples from female Pesisir cattle aged 2-5 years which were obtained from BPTU-HPT Padang Mengatas, West Sumatera. The isolated DNA was then amplified using a pair of primers L and R with PCR technique which produced a fragment with a length of 847 bp. The services of 1st Base Singapore are used for sequencing the amplified product. The results of the research on the identification of the early exon 10 FSHR gene in Pesisir cattle were found that there were 5 SNPs at positions --53T>C, +17A>G, +650C>T, +706A>C and +707 ins>A in some introns 9 to exon 10 first part. The results showed that the genotypic frequency of the Pesisir cattle population was in Hardy-Weinberg imbalance and polymorphisms were found in the early exon 10 FSHR gene diversity.
本研究的目的是鉴定Pessir牛FSHR基因或卵泡刺激激素受体早期外显子10的遗传多样性。所用血液样本为70份2-5岁的雌性Pessir牛的血液样本,这些样本取自西苏门答腊省的BPTU-HPT Padang Mengatas。然后使用一对引物L和R用PCR技术扩增分离的DNA,产生长度为847bp的片段。新加坡第一基地的服务用于扩增产物的测序。Pessir牛早期第10外显子FSHR基因的鉴定研究发现,在第9至第10外隐子第一部分的一些内含子中,有5个SNPs位于53T>C、+17A>G、+650C>T、+706A>C和+707ins>A位置。结果表明,Pessir牛群体的基因型频率处于Hardy-Weinberg不平衡状态,早期外显子10 FSHR基因多样性存在多态性。
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引用次数: 1
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