Pub Date : 2024-06-17DOI: 10.1038/s41684-024-01393-4
Chris Preece, Daniel Biggs, Edward Grencis, Maj Simonsen Jackson, Sue Allen, Martin Fray, Antony Adamson, Benjamin Davies
For the preparation of embryo transfer recipients, surgically vasectomized mice are commonly used, generated by procedures associated with pain and discomfort. Sterile transgenic strains provide a nonsurgical replacement, but their maintenance requires breeding and genotyping procedures. We have previously reported the use of naturally sterile STUSB6F1 hybrids for the production of embryo transfer recipients and found the behavior of these recipients to be indistinguishable from those generated by vasectomized males. The method provides two substantial 3R impacts: refinement (when compared with surgical vasectomy) and reduction in breeding procedures (compared with sterile transgenic lines). Despite initial promise, the 3Rs impact of this innovation was limited by difficulties in breeding the parental STUS/Fore strain, which precluded the wider distribution of the sterile hybrid. The value of a 3R initiative is only as good as the uptake in the community. Here we, thus, select a different naturally sterile hybrid, generated from strains that are widely available: the B6SPRTF1 hybrid between C57BL/6J and Mus spretus. We first confirmed its sterility by sperm counting and testes weight and then trialed the recovery of cryopreserved embryos and germplasm within three UK facilities. Distribution of sperm for the generation of these hybrids by in vitro fertilization was found to be the most robust distribution method and avoided the need to maintain a live M. spretus colony. We then tested the suitability of B6SPRTF1 sterile hybrids for the generation of embryo transfer recipients at these same three UK facilities and found the hybrids to be suitable when compared with surgical vasectomized mice and a sterile transgenic strain. In conclusion, the potential 3Rs impact of this method was confirmed by the ease of distribution and the utility of sterile B6SPRTF1 hybrids at independent production facilities. Preece et al. show that B6SPRTF1 hybrid males between C57BL/6J and Mus spretus are suitable for the generation of pseudopregnant female mice for embryo transfer. By providing an alternative to vasectomized males, the method shows clear 3R benefits.
{"title":"Naturally sterile Mus spretus hybrids are suitable for the generation of pseudopregnant embryo transfer recipients","authors":"Chris Preece, Daniel Biggs, Edward Grencis, Maj Simonsen Jackson, Sue Allen, Martin Fray, Antony Adamson, Benjamin Davies","doi":"10.1038/s41684-024-01393-4","DOIUrl":"10.1038/s41684-024-01393-4","url":null,"abstract":"For the preparation of embryo transfer recipients, surgically vasectomized mice are commonly used, generated by procedures associated with pain and discomfort. Sterile transgenic strains provide a nonsurgical replacement, but their maintenance requires breeding and genotyping procedures. We have previously reported the use of naturally sterile STUSB6F1 hybrids for the production of embryo transfer recipients and found the behavior of these recipients to be indistinguishable from those generated by vasectomized males. The method provides two substantial 3R impacts: refinement (when compared with surgical vasectomy) and reduction in breeding procedures (compared with sterile transgenic lines). Despite initial promise, the 3Rs impact of this innovation was limited by difficulties in breeding the parental STUS/Fore strain, which precluded the wider distribution of the sterile hybrid. The value of a 3R initiative is only as good as the uptake in the community. Here we, thus, select a different naturally sterile hybrid, generated from strains that are widely available: the B6SPRTF1 hybrid between C57BL/6J and Mus spretus. We first confirmed its sterility by sperm counting and testes weight and then trialed the recovery of cryopreserved embryos and germplasm within three UK facilities. Distribution of sperm for the generation of these hybrids by in vitro fertilization was found to be the most robust distribution method and avoided the need to maintain a live M. spretus colony. We then tested the suitability of B6SPRTF1 sterile hybrids for the generation of embryo transfer recipients at these same three UK facilities and found the hybrids to be suitable when compared with surgical vasectomized mice and a sterile transgenic strain. In conclusion, the potential 3Rs impact of this method was confirmed by the ease of distribution and the utility of sterile B6SPRTF1 hybrids at independent production facilities. Preece et al. show that B6SPRTF1 hybrid males between C57BL/6J and Mus spretus are suitable for the generation of pseudopregnant female mice for embryo transfer. By providing an alternative to vasectomized males, the method shows clear 3R benefits.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 7","pages":"181-185"},"PeriodicalIF":5.9,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01393-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141334374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-17DOI: 10.1038/s41684-024-01394-3
Stefano Pluchino, Ivan Lombardi
Understanding the inherent complexity of organogenesis and addressing the persistent shortage of organ donors remain paramount scientific challenges. Recent advances in chimeric blastocyst technology offer promising solutions. Two new pioneering studies have successfully generated functional rat–mouse brain chimeras, providing novel insights into brain development and potential regenerative therapies. However, several technical and ethical hurdles persist.
{"title":"Crossing species boundaries in regenerative neuroscience with rat–mouse brain chimeras","authors":"Stefano Pluchino, Ivan Lombardi","doi":"10.1038/s41684-024-01394-3","DOIUrl":"10.1038/s41684-024-01394-3","url":null,"abstract":"Understanding the inherent complexity of organogenesis and addressing the persistent shortage of organ donors remain paramount scientific challenges. Recent advances in chimeric blastocyst technology offer promising solutions. Two new pioneering studies have successfully generated functional rat–mouse brain chimeras, providing novel insights into brain development and potential regenerative therapies. However, several technical and ethical hurdles persist.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 7","pages":"179-180"},"PeriodicalIF":5.9,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01394-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141334310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-17DOI: 10.1038/s41684-024-01392-5
Jorge Ferreira
{"title":"The love maze allows female marmosets to choose their partner","authors":"Jorge Ferreira","doi":"10.1038/s41684-024-01392-5","DOIUrl":"10.1038/s41684-024-01392-5","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 7","pages":"173-173"},"PeriodicalIF":5.9,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141334312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-04DOI: 10.1038/s41684-024-01386-3
Alexandra Le Bras
{"title":"Role of Evi5 in Drosophila iron metabolism","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01386-3","DOIUrl":"10.1038/s41684-024-01386-3","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 6","pages":"127-127"},"PeriodicalIF":6.9,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141248122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-04DOI: 10.1038/s41684-024-01384-5
Alexandra Le Bras
{"title":"A microminipig model of DMD","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01384-5","DOIUrl":"10.1038/s41684-024-01384-5","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 6","pages":"127-127"},"PeriodicalIF":6.9,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141248076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-04DOI: 10.1038/s41684-024-01387-2
Alexandra Le Bras
{"title":"Role of REM sleep in fear extinction","authors":"Alexandra Le Bras","doi":"10.1038/s41684-024-01387-2","DOIUrl":"10.1038/s41684-024-01387-2","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 6","pages":"127-127"},"PeriodicalIF":6.9,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141248097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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