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Postmortem Examination of Emu Bird (Dromaius novaehollandiae) in Ragunan Zoo 拉古南动物园鸸鹋鸟的尸体检验
Pub Date : 2022-10-23 DOI: 10.20473/jmv.vol5.iss2.2022.252-260
Friselsa Pardede, Nindya Anandaputri Rahardi, S. Edwar, Kusdiantoro Mohamad
Ragunan Zoo is one of the ex-situ conservation sites in DKI Jakarta to protect the wildlife outside their natural habitat. The emu (Dromaius novaehollandiae) is one of the TMR collection animals originating from Australia. An emu bird named Lebo, aged 19 years, and male sex was found sick on January 12, 2022. Based on physical examination, the emu bird showed clinical symptoms of anorexia, unwilling to eat, lethargic, refusing or difficult to move, unable to lift his head, and inflammation of the right eye. No further examination was performed to determine the diagnosis. Treatments were taken symptomatically, namely the fluid therapy, injection of vitamins and energy sources, antibiotics, and anti-inflammatory. The emu was found dead on February 6, 2022 and a necropsy was performed. Postmortem findings obtained were glossitis, ventriculitis, gizzard erosion, hemorrhagic enteritis, hepatic lipidosis, epicarditis, testicular atrophy, and orchitis. Diagnosis of suspected cause of death is damage and decreased function of various organs of the body.
Ragunan动物园是雅加达DKI的迁地保护区之一,以保护野生动物远离自然栖息地。鸸鹋(Dromaius novaehollanddiae)是一种原产于澳大利亚的TMR收集动物。2022年1月12日,一只名叫Lebo的19岁雄性鸸鹋鸟被发现患病。经体格检查,鸸鹋鸟临床表现为厌食、不愿进食、嗜睡、拒绝或行动困难、无法抬起头、右眼发炎。未进行进一步检查以确定诊断。治疗采取对症治疗,即液体疗法、注射维生素和能量源、抗生素和抗炎。这只鸸鹋于2022年2月6日被发现死亡,并进行了尸检。尸检结果为舌炎、脑室炎、砂囊糜烂、出血性肠炎、肝性脂质症、心外膜炎、睾丸萎缩和睾丸炎。疑似死因的诊断是身体各器官的损伤和功能下降。
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引用次数: 0
Isolation and Identification of Salmonella Spp. on Broiler Meat at Banyuwangi and Blambangan Traditional Markets, Banyuwangi Regency Banyuwangi和Blambangan传统市场肉鸡肉中沙门氏菌的分离与鉴定
Pub Date : 2022-10-23 DOI: 10.20473/jmv.vol5.iss2.2022.219-224
Diana Novita Putri, J. Rahmahani, M. Yunita
Salmonella spp. is one of the major causes of the world's foodborne disease that can spread through meats, eggs, or milk. Salmonella spp. is an enteropathogenic bacteria that can cause gastrointestinal infections. This study aimed to identify the contaminations of Salmonella spp. bacteria on the broiler meats at Banyuwangi and Blambangan traditional markets Banyuwangi district. The method of this study used 24 samples of broiler meat bought from Banyuwangi and Blambangan traditional markets in the Banyuwangi District. The samples were collected using the purposive sampling method. The results found that 5 out of 24 samples (21%) were tested positive for Salmonella spp. contamination and 19 were tested negative. This study was indicated that broiler meats at Banyuwangi and Blambangan traditional markets Banyuwangi district have not suitable with SNI (Indonesia's National Standard) 3924:2009 that requires at least 25 gram of fresh chicken meat to be free of or tested negative for Salmonella.
沙门氏菌是世界上食源性疾病的主要原因之一,它可以通过肉类、鸡蛋或牛奶传播。沙门氏菌是一种肠道致病菌,可引起胃肠道感染。本研究旨在对Banyuwangi区Banyuwangi和Blambangan传统市场肉鸡肉中沙门氏菌的污染情况进行调查。本研究方法使用了从Banyuwangi区Banyuwangi和Blambangan传统市场购买的24份肉鸡肉样本。样本采用目的抽样法采集。结果发现,24份样本中有5份(21%)沙门氏菌污染检测呈阳性,19份检测呈阴性。本研究表明,Banyuwangi区Banyuwangi和Blambangan传统市场的肉鸡肉不符合SNI(印度尼西亚国家标准)3924:2009的要求,该标准要求至少25克新鲜鸡肉不含沙门氏菌或检测呈阴性。
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引用次数: 0
Risk Factors and Incidence of Escherichia coli Producing Extended-spectrum β-lactamase (ESBL) in Dairy Cattle 奶牛产广谱β-内酰胺酶(ESBL)的大肠杆菌危险因素及发病率
Pub Date : 2022-10-23 DOI: 10.20473/jmv.vol5.iss2.2022.213-218
F. Dameanti, Muhammad Ali Akramsyah, Chyntia Silvi Yanti Hasan, Jacky Teguh Amanda, Alfaro Rikko Pratama, Reza Fahmiantika, Dhaneswara Tedja, Safira Izofani, Rahayu Sutrisno
This study aimed to evaluate the risk factor and insidence of Extended-spectrum b-lactamase (ESBL) produced by Escherichia coli in dairy cattle. The samples obtained were 38 rectal swabs from dairy cows from the Successful Mutual Livestock Group, Deyeng Village, Kediri Regency, East Java. The study started with interviews and observations using a questionnaire to obtain data on risk factors (source of drinking water, cleanliness of feedlots, cleanliness of drinking places, administration of antibiotics, history of mastitis). A sampling of the rectal swab of cattle was carried out using Nutrient Broth as carrier media. Isolation of bacteria using Mac Conkey Agar (MCA) media with the antibiotic cefotaxime 1 mg/L. The addition of antibiotics is intended so that the bacteria that grow are coliform bacteria resistant to the cefotaxime antibiotic, a beta-lactam group. Bacterial isolation was continued with MCA media to enrich ESBL bacteria. Bacterial identification was continued on EMBA media, gram staining, IMViC biochemical test, TSIA, and urease to obtain isolates of E. coli bacteria. The E. coli isolates tested for ESBL Double Disc Synergy Test (DDST) phenotype. The close relationship between each risk factor and the incidence of ESBL-producing E. coli was tested statistically using the Spearman Rank correlation test. The results showed that the incidence of ESBL-producing E. coli in the tested samples was 21.05%, or there were eight positive samples of ESBL-producing E. coli. The risk factors for finding a positive incidence of E. coli producing ESBL in this study were 87% of the use of drinking water sources from wells, 25% did not pay attention to the cleanliness of drinking places, 25% did not pay attention to the cleanliness of eating places, 38% were given antibiotics during maintenance, and 100% had a history of mastitis. These risk factors were not statistically closely related to the incidence of ESBL-producing E. coli.
本研究旨在探讨大肠杆菌在奶牛体内产生的广谱b-内酰胺酶(ESBL)的危险因素及其内在特征。获得的样本是来自东爪哇Kediri Regency Deyeng村成功互助畜牧集团奶牛的38头直肠拭子。该研究从访谈和观察开始,通过问卷调查获得风险因素的数据(饮用水来源、饲养场的清洁度、饮用场所的清洁度、抗生素的使用、乳腺炎的历史)。以营养肉汤为载体,对牛直肠拭子进行了取样。用抗生素头孢噻肟1mg /L的Mac Conkey琼脂(MCA)培养基分离细菌。添加抗生素的目的是使生长的细菌是对头孢噻肟抗生素(一种-内酰胺类抗生素)具有耐药性的大肠菌群。继续用MCA培养基进行细菌分离,以丰富ESBL细菌。继续在EMBA培养基、革兰氏染色、IMViC生化试验、TSIA和脲酶上进行细菌鉴定,以获得大肠杆菌分离株。分离的大肠杆菌进行了ESBL双盘协同试验(DDST)表型检测。各危险因素与产esbl大肠杆菌发病率之间的密切关系采用Spearman秩相关检验进行统计学检验。结果显示,检测样品中产esbl大肠杆菌的发生率为21.05%,有8份产esbl大肠杆菌阳性样品。本研究发现产ESBL大肠杆菌阳性的危险因素为:使用水井饮用水源占87%,不注意饮水场所清洁度占25%,不注意饮食场所清洁度占25%,维持期间使用抗生素占38%,有乳腺炎史占100%。这些危险因素与产esbl大肠杆菌的发病率没有统计学上的密切关系。
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引用次数: 0
Surgery Management Post Ovulatory Egg Stasis in Corn Snake (Pantherophis guttatus) 玉米蛇(Pantherophis guttatus)排卵后卵瘀的手术处理
Pub Date : 2022-10-23 DOI: 10.20473/jmv.vol5.iss2.2022.261-265
D. Sari, Desty Apritya, Muhammad Noor Rahman
Post Ovulatory Egg Stasis or dystocia is difficulty releasing eggs. The incident was experienced by a female snake due to an obstructive tract. A four years old corn snake (Pantherophis guttatus) female with clinical symptoms that appear are anorexia for two weeks. The condition of the coelomic cavity is still large after 7 days of laying eggs. The results of physical examination showed that the snake had a distended coelomic cavity, pale mucous membrane, hard and smooth palpation when palpated. The results of x-ray examination showed that there was an oval radiopaque formation in the coelomic cavity. Coeliotomy surgery is performed to remove the eggs by the salpingotomy method. The general anesthetic used was a combination of zolazepam-tiletamine at a dose of 10 mg/kg body weight and meloxicam 0,1 mg/kg body weight was given. Postoperative therapy was oxytetracycline 10 mg/kg body weight, dexamethasone 5 mg/kg body weight and vigantol E® 0,1 mg/kg body weight. The general condition of the snake after 5 days improved and the wound had dried up. Then 2 weeks after surgery the stitches in the skin were removed when shedding of the skin.
排卵后卵子停滞或难产是排卵困难。这一事件发生在一条雌性蛇身上,原因是它的呼吸道梗阻性阻塞。4岁玉米蛇(Pantherophis guttatus)雌性,临床表现为厌食2周。产蛋7天后体腔状况仍较大。体格检查结果显示,该蛇体腔肿胀,粘膜苍白,触诊时硬而光滑。x线检查显示体腔内有卵圆形不透射线形成。通过输卵管切开术切除卵子。全身麻醉剂为唑拉西泮-替乐胺(10mg /kg体重)和美洛昔康(0.1 mg/kg体重)的组合。术后给予土霉素10 mg/kg体重,地塞米松5 mg/kg体重,维安妥尔E®0.1 mg/kg体重。5天后蛇的一般情况有所改善,伤口已经干涸。术后2周,在皮肤脱落时,将皮肤上的缝线拆除。
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引用次数: 0
Total Plate Count of Broiler Meat at Various Market in Bekasi City in March 2021 2021年3月勿加西市各市场肉鸡总盘数
Pub Date : 2022-10-23 DOI: 10.20473/jmv.vol5.iss2.2022.157-161
Aulia Amatul Firdausyi, A. Estoepangestie, Prima Ayu Wibawati, I. S. Hamid, T. Solikhah, R. Prastiya
Food-safety of broiler meat is important because broiler meat is a foodstuff that can be easily contaminated by biological hazard such as bacteria. This study aimed to determine the total plate count of broiler meat at various market in Bekasi City in March 2021. Amount of samples in this study were 40 samples of broiler meat from 10 markets, samples were taken purposively and each sample used 25 grams from Musculus pectoralis of broiler meat. Samples were examined using pour plate method of total plate count. The result showed that 80% (32 of 40 samples) were exceeded the TPC’s limit. The highest value of TPC result was 3,7 × 107 CFU/g from “H” market.
肉鸡肉是一种容易被细菌等生物危害污染的食品,因此肉鸡肉的食品安全至关重要。本研究旨在确定2021年3月勿加西市各市场肉鸡总盘数。本研究的样本数量为来自10个市场的40份肉鸡肉样本,有目的地采集样本,每个样本取自肉鸡胸肌25克。样品采用总板计数的倒板法进行检测。结果表明,40份样品中有32份(80%)的TPC含量超标。产自“H”市场的TPC结果最高为3.7 × 107 CFU/g。
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引用次数: 0
Stall Sanitation Associated with Cryptosporidium sp. Infection on Calf and Cattle Farmer 牛棚卫生与犊牛隐孢子虫感染的关系
Pub Date : 2022-10-23 DOI: 10.20473/jmv.vol5.iss2.2022.178-187
Rafindra Ramadhani, Y. Nurdian, D. Rachmawati, W. S. Utami, Yunita Armiyanti, Bagus Hermansyah, Angga Mardro Rahardjo
Increasing the cattle population can increase the amount of the waste. Poor management of cage sanitation has the risk of causing a decrease in the health conditions of cattle farmers, such as: diarrhea, nausea, vomiting, and itching, as many as 50% of cattle with diarrhea are infected with Cryptosporidium sp. The highest infection of Cryptosporidium sp. in cattle found in calves. The calf can excrete 1×1010 oocysts of Cryptosporidium sp. per day. Cryptosporidium sp. also has the potential to cause zoonotic infections that are transmitted from animals to humans. The study aims was to determine the relationship between stall sanitation and Cryptosporidium sp. to calf and cattle farmer. The type of this research was analytic observational with a cross sectional approach conducted in Jatian Village, Pakusari, Jember during October 2021 - March 2022. The primary data was obtained through interviews and observations. The feces of calves and cattle farmer were collected and examined using sedimentation method and modified Ziehl-Neelsen (MZN) staining method. The statistical test used is the chi-square test. The results showed that the prevalence of Cryptosporidium sp. in calves by 14,3% and on cattle farmer by 4,8%. The bivariate analysis showed that that stall location and waste handling were associated with Cryptosporidium for calf (p≤0.05), and there were no variables that were significantly related to infection in cattle farmers (p>0,05). Cryptosporidium sp. in calves and humans can be caused by several factors, such as environmental factors and hosts. Preventive measures such as maintaining a clean environment including cowshed sanitation and personal hygiene need to be taken to reduce the risk of Cryptosporidium infection from calves to humans, and vice versa.
增加牛的数量会增加废物的数量。笼内卫生管理不善有可能导致养牛户的健康状况下降,例如:腹泻、恶心、呕吐和瘙痒,多达50%的腹泻牛感染隐孢子虫。牛隐孢子虫感染率最高的是小牛。犊牛每天可排泄1×1010隐孢子虫卵囊。隐孢子虫也有可能引起从动物传染给人类的人畜共患感染。研究的目的是确定畜栏卫生与隐孢子虫对小牛和养牛户的关系。本研究采用横断面分析观察方法,于2021年10月至2022年3月在Jember的Pakusari Jatian村进行。主要数据是通过访谈和观察获得的。采用沉淀法和改良Ziehl-Neelsen (MZN)染色法采集犊牛和牛农粪便进行检测。使用的统计检验是卡方检验。结果表明,犊牛隐孢子虫感染率为14.3%,养牛户感染率为4.8%。双变量分析结果显示,畜栏位置和粪便处理与犊牛隐孢子虫感染相关(p≤0.05),与养牛户感染无显著相关(p> 0.05)。小牛和人类的隐孢子虫可由多种因素引起,如环境因素和宿主。需要采取预防措施,如保持环境清洁,包括牛棚卫生和个人卫生,以减少从小牛到人的隐孢子虫感染的风险,反之亦然。
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引用次数: 0
Difference Time Effect of Equilibration Before Freezing on The Quality of Spermatozoa Sapera Goats using Egg Yellow Tris 冷冻前平衡对黄卵黄山羊精子质量的时间差效应
Pub Date : 2022-10-23 DOI: 10.20473/jmv.vol5.iss2.2022.188-195
Danu Prayogo, S. Susilowati, R. Prastiya, E. Safitri, B. Agustono
This study aimed to examine the effect of time differences on motility, viability, abnormality, and intact plasma membrane of spermatozoa of Sapera goats before freezing. This study used a Completely Randomized Design (CRD) with three settings, namely the equilibration time P1 (1 h), P2 (3 h), and P3 (4 h), each of six replications. The results of motility using One Way Anova statistical analysis through the Duncan Test were (P1) 71.50 ± 3.94, (P2) 66.00 ± 2.60, and (P3) 60.33 ± 2.42. The results of viability was (P1) 83.00 ± 6.16, (P2) 75.17 ± 4.70, and (P3) 69.00 ± 3.40. Abnormal results was (P1) 2.50 ± 0.55, (P2) 3.33 ± 1.18, and (P3) 6.50 ± 2.88. While the results of intact plasma membranes were (P1) 79.50 ± 8.50, (P2) 72.67 ± 5.28, and (P3) 65.67 ± 3.78. The conclusion of the study showed significant significant results (p<0,05) between the equilibration time and spermatozoa quality of Sapera goats before freezing. The highest presentation of motility, viability and overall plasma membrane was at an equilibration time of 1 h, while the lowest abnormality presentation was at equilibration time of 1 h.
本研究旨在探讨冷冻前时间差异对黑山羊精子活力、活力、异常和质膜完整程度的影响。本研究采用完全随机设计(CRD),设置平衡时间P1 (1 h)、P2 (3 h)和P3 (4 h) 3个设置,每6个重复。Duncan检验单因素方差分析结果为(P1) 71.50±3.94,(P2) 66.00±2.60,(P3) 60.33±2.42。存活率分别为(P1) 83.00±6.16,(P2) 75.17±4.70,(P3) 69.00±3.40。异常结果为(P1) 2.50±0.55,(P2) 3.33±1.18,(P3) 6.50±2.88。完整质膜检测结果为(P1) 79.50±8.50,(P2) 72.67±5.28,(P3) 65.67±3.78。研究结果表明,冷冻前平衡时间与精子质量之间存在显著性差异(p< 0.05)。活力、活力和整体质膜的异常表现在平衡时间为1 h时最高,而异常表现在平衡时间为1 h时最低。
{"title":"Difference Time Effect of Equilibration Before Freezing on The Quality of Spermatozoa Sapera Goats using Egg Yellow Tris","authors":"Danu Prayogo, S. Susilowati, R. Prastiya, E. Safitri, B. Agustono","doi":"10.20473/jmv.vol5.iss2.2022.188-195","DOIUrl":"https://doi.org/10.20473/jmv.vol5.iss2.2022.188-195","url":null,"abstract":"This study aimed to examine the effect of time differences on motility, viability, abnormality, and intact plasma membrane of spermatozoa of Sapera goats before freezing. This study used a Completely Randomized Design (CRD) with three settings, namely the equilibration time P1 (1 h), P2 (3 h), and P3 (4 h), each of six replications. The results of motility using One Way Anova statistical analysis through the Duncan Test were (P1) 71.50 ± 3.94, (P2) 66.00 ± 2.60, and (P3) 60.33 ± 2.42. The results of viability was (P1) 83.00 ± 6.16, (P2) 75.17 ± 4.70, and (P3) 69.00 ± 3.40. Abnormal results was (P1) 2.50 ± 0.55, (P2) 3.33 ± 1.18, and (P3) 6.50 ± 2.88. While the results of intact plasma membranes were (P1) 79.50 ± 8.50, (P2) 72.67 ± 5.28, and (P3) 65.67 ± 3.78. The conclusion of the study showed significant significant results (p<0,05) between the equilibration time and spermatozoa quality of Sapera goats before freezing. The highest presentation of motility, viability and overall plasma membrane was at an equilibration time of 1 h, while the lowest abnormality presentation was at equilibration time of 1 h.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80173306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Efficacy of Grass and Concentrate Ratio on Milk Urea Nitrogen, Progesterone, and Pregnancy Status in Friesian Holstein 草精比对荷斯坦黑马乳尿素氮、孕酮及妊娠状况的影响
Pub Date : 2022-10-23 DOI: 10.20473/jmv.vol5.iss2.2022.207-212
Enggar Hardaningtyas, Imam Mustofa, S. Madyawati, R. Rimayanti, E. Safitri, H. A. Hermadi
This study was conducted to know the influence of grass to concentrate ratio to milk urea nitrogen (MUN) level, progesterone level and pregnancy status in Friesian Holstein. A total of 18 dairy cows were randomly selected from the population. The cows were grouped based on feed and MUN and pregnancy. Milk sample collection was carried out once on the seventh day after estrus (D7) and tested with the Barthelot method for measuring urea nitrogen levels. Blood samples were taken three times, on the day of estrus (D0), seven days after estrus (D7), and twenty-two days after estrus (D22), and were tested using Immuno-Sorbent Assay (ELISA). Pregnancy examination was carried out at D90 after artificial insemination through rectal palpation. Based on the analysis, grass to concentrate ratio, progesterone level and MUN level showed no significant difference (p>0.05) but it showed significantly different on milk yield (p<0,05). Based on pregnancy status, progesterone level at D22 showed significantly different whereas at D0 and D7 showed insignificantly different. MUN level and grass to concentrate level also showed significantly different. It can be concluded that the ratio of grass and concentrate did not increase MUN level, progesterone level and pregnancy rate.
本试验旨在了解草精比与乳尿素氮(MUN)水平、孕酮水平和妊娠状况对荷斯坦黑马的影响。从种群中随机选取18头奶牛。根据饲料、MUN和怀孕情况对奶牛进行分组。于发情后第7天(D7)采集1次乳样,采用Barthelot法测定尿素氮水平。分别于发情当天(D0)、发情后第7天(D7)、发情后第22天(D22)三次采血,采用免疫吸附试验(ELISA)进行检测。人工授精后D90行直肠触诊妊娠检查。经分析,草精比、孕酮水平和MUN水平差异不显著(p>0.05),但产奶量差异显著(p< 0.05)。不同妊娠状态下,孕酮水平在D22时差异显著,而在D0和D7时差异不显著。MUN水平和草精水平也有显著差异。综上所述,草料与精料之比对小鼠MUN水平、孕酮水平及妊娠率均无显著影响。
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引用次数: 0
Clinical Signs and Haematology Profiles of Beagle Dogs with Tick Infestation 比格犬蜱虫感染的临床特征和血液学特征
Pub Date : 2022-10-23 DOI: 10.20473/jmv.vol5.iss2.2022.266-269
A. P. Mihardi, Jayusman Ayu Joesoef, Kathirina Beathrik Riwu Wolo, A. Esfandiari, S. D. Widhyari, R. Wulansari, Agus Wijaya, L. Maylina
Rhipicephalus sanguineus is one of the vectors that could induce diseases in dogs. A physical examination and haematology test could examine the dog's condition with tick infestation. Five dogs, beagle breeds from Veterinary Teaching Hospital, School of Veterinary Medicine and Biosciences, IPB University, have been examined for physical examination and CBC test. A haematology test was performed using a haematology analyzer and made the blood smear. Physical examination showed that 60% of dogs have a tick infestation, 80% have pallor, 60% have pruritus, 40% have crusted skin, and 100% have wounds in extremities. However, the body temperature and heart rate were relatively normal. Complete blood count test showed that red blood cells (RBC), peak cells volume, and haemoglobin (Hb) decreased (80%), MCHC increased (40%), thrombocytopenia (100%), leukopenia (40%), lymphocytopenia (60%), neutropenia (10%) and monositopenia (80%). The blood smear result showed all dogs have positive blood parasites, with the highest percentage being the third dog (0.056%). From the physical examination, CBC, and blood smear, it can be concluded that dogs with tick infestation would experience anaemia that showed by RBC, PCV, decreasing number of platelet and positive blood parasite.
血鼻头虫是能引起犬类疾病的媒介之一。身体检查和血液学测试可以检查狗是否感染蜱虫。对IPB大学动物医学与生物科学学院兽医教学医院的5只小猎犬进行了体格检查和全血细胞计数测试。使用血液学分析仪进行血液学测试并进行血液涂片。体格检查显示,60%的狗有蜱虫感染,80%的狗面色苍白,60%的狗有瘙痒,40%的狗皮肤结痂,100%的狗四肢有伤口。然而,体温和心率相对正常。全血细胞计数检查显示红细胞(RBC)、峰值细胞体积、血红蛋白(Hb)降低(80%),MCHC升高(40%),血小板减少(100%)、白细胞减少(40%)、淋巴细胞减少(60%)、中性粒细胞减少(10%)和单核细胞减少(80%)。血涂片结果显示所有犬血寄生虫阳性,其中以第3只犬血寄生虫阳性比例最高(0.056%)。通过体格检查、全血细胞计数和血涂片检测,发现蜱虫感染的犬会出现贫血,表现为红细胞、PCV、血小板数量减少和血寄生虫阳性。
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引用次数: 1
Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method 聚合酶链反应法诊断肉鸡包涵体肝炎
Pub Date : 2022-10-14 DOI: 10.20473/jmv.vol5.iss2.2022.145-148
J. Rahmahani, Maha Kirana, F. Rantam, S. Suwarno
This study aimed to diagnose cases of Inclusion Bodies Hepatitis (IBH) in broiler chickens. A total of 6 chickens were collected from farms in Banten and Jember. Samples were taken from the liver which underwent changes such as brittle, yellowish in color and enlarged. The samples were ground and extracted, then the Polymerase Chain Reaction (PCR) test was performed using Primers Hexon A (5'-CAARTTCAGRCAGACGGT-3') and Hexon B ( 5'-TAGTGATGMCGSGACATCAT-3' ) with 897 bp length. The results showed that the broiler chicken case sample from Banten was positively 100% infected by the IBH Virus.
本研究旨在对肉鸡包涵体肝炎(IBH)进行诊断。从万丹和余月的农场共收集了6只鸡。样本取自肝脏,肝脏变脆、变黄、变大。样品研磨后提取,用长度为897 bp的引物Hexon A(5’- caarttcagagrcagacggt -3’)和Hexon B(5’-TAGTGATGMCGSGACATCAT-3’)进行聚合酶链反应(PCR)检测。结果表明,万腾肉鸡病例标本IBH病毒感染阳性率为100%。
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引用次数: 1
期刊
Jurnal Medik Veteriner
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