Pub Date : 2022-04-22DOI: 10.20473/jmv.vol5.iss1.2022.1-8
A. L. Saputro, R. Prastiya, Meidina Zulva Ulinuha, Prasita Widayani
The purpose of this study was to determine the effectiveness of time equilibration before freezing sapera goat spermatozoa after ESS (Electric Separating Sperm) on motility, viability, membrane integrity, normality and abnormality of spermatozoa. Sapera goat spermatozoa were collected using an artificial vagina and then separated using the ESS method. This study used a completely randomized formula with three treatments, which are the equilibration time of P1 (1 hour), P2 (3 hours), and P3 (4 hours), with six replications. The analysis in this study used the ANOVA test followed by the Duncan test to determine the real difference between treatments. The conclusion of this study showed the highest percentage of motility, viability, membrane integrity, normality of sapera goat spermatozoa at 1-hour treatment, for the lowest percentage of abnormalities at 1-hour treatment. Significantly different results (p <0.05) on the anode and cathode sides in all equilibration time treatments.
{"title":"The Effectiveness of Time Equilibration Before Freezing in Sapera Goat Spermatozoa After Electric Separating Sperm","authors":"A. L. Saputro, R. Prastiya, Meidina Zulva Ulinuha, Prasita Widayani","doi":"10.20473/jmv.vol5.iss1.2022.1-8","DOIUrl":"https://doi.org/10.20473/jmv.vol5.iss1.2022.1-8","url":null,"abstract":"The purpose of this study was to determine the effectiveness of time equilibration before freezing sapera goat spermatozoa after ESS (Electric Separating Sperm) on motility, viability, membrane integrity, normality and abnormality of spermatozoa. Sapera goat spermatozoa were collected using an artificial vagina and then separated using the ESS method. This study used a completely randomized formula with three treatments, which are the equilibration time of P1 (1 hour), P2 (3 hours), and P3 (4 hours), with six replications. The analysis in this study used the ANOVA test followed by the Duncan test to determine the real difference between treatments. The conclusion of this study showed the highest percentage of motility, viability, membrane integrity, normality of sapera goat spermatozoa at 1-hour treatment, for the lowest percentage of abnormalities at 1-hour treatment. Significantly different results (p <0.05) on the anode and cathode sides in all equilibration time treatments.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80606619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-22DOI: 10.20473/jmv.vol5.iss1.2022.28-33
J. Rahmahani, Daniswara Irwanza Akbar, S. Suwarno
The aim of this study was to compare the antigenicity between the whole virus and protein S of both local strain I-147 and Massachusetts strain of Infectious bronchitis virus with indirect ELISA. We used 10 positive serums from post vaccinated chickens for further reaction with four groups of antigens in this study that are: P1) S protein (spike glycoprotein) antigen of local strain IB virus; P2) S protein (spike glycoprotein) antigen of Massachusetts strain IB virus; P3) whole virus antigen of local strain IB virus; P4) whole virus antigen of Massachusetts strain IB virus. The results of this research are Optical Density (OD) values of each treatment, then to be analyzed using One Way ANOVA and followed by Duncan Multiple Range Test 5%. The results show that there are differences between the antigenicity of the whole virus and S protein in both local strain and Massachusetts strain of IB virus. The whole virus antigen of both strains has a significant difference (p<0.05) with the S protein antigen in both strains. Specifically, the whole virus antigen of Massachusetts strain has the highest OD among the other groups.
本研究的目的是用间接ELISA法比较传染性支气管炎本地株I-147和马萨诸塞州株全病毒和蛋白S的抗原性。在本研究中，我们用接种后的10只鸡的阳性血清与四组抗原进行进一步反应:P1)本地株IB病毒S蛋白(刺突糖蛋白)抗原;麻州株IB病毒P2) S蛋白(刺突糖蛋白)抗原;P3)本地株IB病毒全病毒抗原;麻州IB病毒株全病毒抗原。本研究结果为各处理的光密度(OD)值，采用单因素方差分析(One Way ANOVA)进行分析，并采用Duncan多元极差检验5%进行分析。结果表明，IB病毒本地株和麻州株的全病毒抗原性和S蛋白抗原性存在差异。两株的全病毒抗原与S蛋白抗原差异有统计学意义(p<0.05)。其中，麻州毒株的全病毒抗原OD值在其他毒株中最高。
{"title":"Comparison of Antigenicity Between Local and Massachusetts Strains of Infectious Bronchitis Virus using Indirect ELISA Test","authors":"J. Rahmahani, Daniswara Irwanza Akbar, S. Suwarno","doi":"10.20473/jmv.vol5.iss1.2022.28-33","DOIUrl":"https://doi.org/10.20473/jmv.vol5.iss1.2022.28-33","url":null,"abstract":"The aim of this study was to compare the antigenicity between the whole virus and protein S of both local strain I-147 and Massachusetts strain of Infectious bronchitis virus with indirect ELISA. We used 10 positive serums from post vaccinated chickens for further reaction with four groups of antigens in this study that are: P1) S protein (spike glycoprotein) antigen of local strain IB virus; P2) S protein (spike glycoprotein) antigen of Massachusetts strain IB virus; P3) whole virus antigen of local strain IB virus; P4) whole virus antigen of Massachusetts strain IB virus. The results of this research are Optical Density (OD) values of each treatment, then to be analyzed using One Way ANOVA and followed by Duncan Multiple Range Test 5%. The results show that there are differences between the antigenicity of the whole virus and S protein in both local strain and Massachusetts strain of IB virus. The whole virus antigen of both strains has a significant difference (p<0.05) with the S protein antigen in both strains. Specifically, the whole virus antigen of Massachusetts strain has the highest OD among the other groups.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83191199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-31DOI: 10.20473/jmv.vol4.iss2.2021.213-220
Tisya Yumn Al-Zuhroh, K. P. Santoso, M. Yunita, Nove Hidajati, Ratih Novita Praja
This study aimed to analyze the effect of methanol robusta coffee bean extract (Coffea canephora) on liver histopathological description in mice induced with monosodium glutamate (MSG). This study was used 20 male mice for 5 treatment groups: K + (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) per orally for 42 days. Liver histological scores were analyzed using Kruskal Wallis test. The results of Kruskal Wallis test scored p=0,391 which indicates that the data is not significant (p>0.05). In the control and treatment groups there were no significant. In conclusion, the giving methanol robusta coffee bean extract did not show a decrease in hepatosite damage that was different from the negative control treatment group.
{"title":"Necrosis Description of Mice Liver Induced with Monosodium Glutamate and Methanol Robusta Coffee Bean Extract (Coffea canephora)","authors":"Tisya Yumn Al-Zuhroh, K. P. Santoso, M. Yunita, Nove Hidajati, Ratih Novita Praja","doi":"10.20473/jmv.vol4.iss2.2021.213-220","DOIUrl":"https://doi.org/10.20473/jmv.vol4.iss2.2021.213-220","url":null,"abstract":"This study aimed to analyze the effect of methanol robusta coffee bean extract (Coffea canephora) on liver histopathological description in mice induced with monosodium glutamate (MSG). This study was used 20 male mice for 5 treatment groups: K + (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) per orally for 42 days. Liver histological scores were analyzed using Kruskal Wallis test. The results of Kruskal Wallis test scored p=0,391 which indicates that the data is not significant (p>0.05). In the control and treatment groups there were no significant. In conclusion, the giving methanol robusta coffee bean extract did not show a decrease in hepatosite damage that was different from the negative control treatment group.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"196 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75770142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-31DOI: 10.20473/jmv.vol4.iss2.2021.199-206
Agung Jati Kusuma, E. Safitri, Ratih Novita Praja, W. Tyasningsih, M. Yunita, Prima Ayu Wibawati
Brucellosis was an infectious disease caused by the genus brucella. Brucellosis in dairy cattle was caused by Brucella abortus that impaction an abortion. The aim of this study was to determine the presence of antibodies of the Brucella abortus in adult female dairy cattle in the Puspo district Pasuruan using Rose Bengal Test (RBT) and Complement Fixation Test (CFT). A descriptive analysis method with quantitative approach was used in this study. Meanwhile, interviews were conducted to get supporting information from the farmer. Used 100 samples. The results of the study showed 2% samples considered positive brucellosis tested by RBT. To confidence false-positive result, RBT test was followed by CFT. After tested by CFT, in this study there were no brucella antibodies in adult female dairy cattle in Puspo district, Pasuruan.
{"title":"Detection of Antibodies Brucella abortus in dairy cattle in Puspo District, Pasuruan using Rose Bengal Test and Complement Fixation Test","authors":"Agung Jati Kusuma, E. Safitri, Ratih Novita Praja, W. Tyasningsih, M. Yunita, Prima Ayu Wibawati","doi":"10.20473/jmv.vol4.iss2.2021.199-206","DOIUrl":"https://doi.org/10.20473/jmv.vol4.iss2.2021.199-206","url":null,"abstract":"Brucellosis was an infectious disease caused by the genus brucella. Brucellosis in dairy cattle was caused by Brucella abortus that impaction an abortion. The aim of this study was to determine the presence of antibodies of the Brucella abortus in adult female dairy cattle in the Puspo district Pasuruan using Rose Bengal Test (RBT) and Complement Fixation Test (CFT). A descriptive analysis method with quantitative approach was used in this study. Meanwhile, interviews were conducted to get supporting information from the farmer. Used 100 samples. The results of the study showed 2% samples considered positive brucellosis tested by RBT. To confidence false-positive result, RBT test was followed by CFT. After tested by CFT, in this study there were no brucella antibodies in adult female dairy cattle in Puspo district, Pasuruan.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85488327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-31DOI: 10.20473/jmv.vol4.iss2.2021.281-284
Rizal Maulana Ishaq, S. Suharsono, N. Harijani, S. Hidanah, Imam Mustofa
This study was conducted to find out how much losses the breeders get when their cattle experience a decline in day open (DO), service per conception (S/C), and calving interval (CI).There were 108 lactating dairy cows from the dairy farm, Wagir District, Malang Regency.The data that has been taken is then processed by analysis using linear regression method.The results of the analysis prove that reproductive management has an effect of 82% on farmer expenditure.For each setback DO per day, the farmer pays Rp. 609,05.Meanwhile, for each additional S/C the farmer pays Rp. 99,921.7.For each setback the farmer CI will incur a fee of IDR 228,54 / day.So that the equation found is expendses = 609,05 (DO) + 99,921.7 (s / c) +228,54 (CI).
本研究旨在了解当奶牛的开放日(DO)、单胎产仔数(S/C)和产犊间隔(CI)下降时，养殖户的损失有多大。来自玛琅县瓦格尔县奶牛场的108头泌乳奶牛。然后使用线性回归方法对所采集的数据进行分析处理。分析结果证明，生殖管理对农民支出的影响达82%。对于每天的每个挫折DO，农民支付609,05卢比。同时，每增加一份S/C，农民要支付99,921.7卢比。对于每一次挫折，农民CI将产生228,54印尼盾/天的费用。因此，得出的方程是:费用= 609,05 (DO) + 99,921.7 (s / c) +228,54 (CI)。
{"title":"Correlation of Reproduction Management on Losses of Dairy Farmers in Wagir District, Malang Regency","authors":"Rizal Maulana Ishaq, S. Suharsono, N. Harijani, S. Hidanah, Imam Mustofa","doi":"10.20473/jmv.vol4.iss2.2021.281-284","DOIUrl":"https://doi.org/10.20473/jmv.vol4.iss2.2021.281-284","url":null,"abstract":"This study was conducted to find out how much losses the breeders get when their cattle experience a decline in day open (DO), service per conception (S/C), and calving interval (CI).There were 108 lactating dairy cows from the dairy farm, Wagir District, Malang Regency.The data that has been taken is then processed by analysis using linear regression method.The results of the analysis prove that reproductive management has an effect of 82% on farmer expenditure.For each setback DO per day, the farmer pays Rp. 609,05.Meanwhile, for each additional S/C the farmer pays Rp. 99,921.7.For each setback the farmer CI will incur a fee of IDR 228,54 / day.So that the equation found is expendses = 609,05 (DO) + 99,921.7 (s / c) +228,54 (CI).","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77672736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-31DOI: 10.20473/jmv.vol4.iss2.2021.256-268
Yesha Ainesis El Gracianita Pelupessy, P. Wiradana, I. Rosiana, I. G. Widhiantara
Sea turtles in Bali has been exploited excessively, which caused decrease in population, and this issue has placed sea turtles threatened to extinction. This review article aimed to provide information regarding the status, trends, and potential of sea turtles in Bali. Information given in this article is vital to ensure reliable knowledge not to only understand our current situation, but also to increase efficiency in reliant to the problem sea turtles face. The literature study method is used to write this review paper, namely by accessing a number of research paper published nationally and internationally. It was explained that the status of sea turtles is known to be endangered and law enforcement is not sufficient overcoming the problem. Conservation trends such as nurturing hatchlings have shown to not only help restore the population of sea turtles, but also to bring economic benefits to the conservation sites and the people. Potentials of ecotourism and DNA Barcoding has shown to be effective to benefit the people economically and increase the efficiency of law enforcement and conservation. Solutions and methods of improvement such as ecotourism and DNA Barcoding explained in this article is practical for Bali to adapt, so that sea turtle conservation is capable to overcome its status and incline to its potential.
{"title":"Status, Trends, and Potentials of Turtle Conservation in Bali: A Mini Review","authors":"Yesha Ainesis El Gracianita Pelupessy, P. Wiradana, I. Rosiana, I. G. Widhiantara","doi":"10.20473/jmv.vol4.iss2.2021.256-268","DOIUrl":"https://doi.org/10.20473/jmv.vol4.iss2.2021.256-268","url":null,"abstract":"Sea turtles in Bali has been exploited excessively, which caused decrease in population, and this issue has placed sea turtles threatened to extinction. This review article aimed to provide information regarding the status, trends, and potential of sea turtles in Bali. Information given in this article is vital to ensure reliable knowledge not to only understand our current situation, but also to increase efficiency in reliant to the problem sea turtles face. The literature study method is used to write this review paper, namely by accessing a number of research paper published nationally and internationally. It was explained that the status of sea turtles is known to be endangered and law enforcement is not sufficient overcoming the problem. Conservation trends such as nurturing hatchlings have shown to not only help restore the population of sea turtles, but also to bring economic benefits to the conservation sites and the people. Potentials of ecotourism and DNA Barcoding has shown to be effective to benefit the people economically and increase the efficiency of law enforcement and conservation. Solutions and methods of improvement such as ecotourism and DNA Barcoding explained in this article is practical for Bali to adapt, so that sea turtle conservation is capable to overcome its status and incline to its potential.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"388 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75528412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-31DOI: 10.20473/jmv.vol4.iss2.2021.269-274
Rahmadani Rahmadani, K. Kusnoto, Aditya Yudhana
This study aimed to explain the case of ectoparasite infection and the life cycle of parasites in turtledoves (Geopelia striata). Sarcoptes sp. one of the ectoparasites belonging to the phylum Arthropods, the Arachnid class, and the order Acarina.Sarcoptes sp. can infect the skin of the host through direct contact with infected animals and the environment. Sarcoptes sp. infects by forming a lump under the skin and severe infection causes a crust to form on the skin and is accompanied by itching. Physical examination was carried out by means of inspection of the infected skin area. Subsequent examinations were carried out on the dead skin cells mixed with fresh manure macroscopically and microscopically. Macroscopic examination was done by observing the condition of the skin that is discraped. Microscopic examination using native examination. Based on the negative examination, it was found that there were Sarcoptes sp. mites on an objective microscope with a 40x magnification. Geopelia striata can be infected with Sarcoptes sp. through direct contact with sick animals. The incubation period of Sarcoptes sp. after infection from infected animals was 3-6 weeks.
{"title":"Sarcoptes sp. Infection in Turtledoves (Geopelia striata) at Banyuwangi","authors":"Rahmadani Rahmadani, K. Kusnoto, Aditya Yudhana","doi":"10.20473/jmv.vol4.iss2.2021.269-274","DOIUrl":"https://doi.org/10.20473/jmv.vol4.iss2.2021.269-274","url":null,"abstract":"This study aimed to explain the case of ectoparasite infection and the life cycle of parasites in turtledoves (Geopelia striata). Sarcoptes sp. one of the ectoparasites belonging to the phylum Arthropods, the Arachnid class, and the order Acarina.Sarcoptes sp. can infect the skin of the host through direct contact with infected animals and the environment. Sarcoptes sp. infects by forming a lump under the skin and severe infection causes a crust to form on the skin and is accompanied by itching. Physical examination was carried out by means of inspection of the infected skin area. Subsequent examinations were carried out on the dead skin cells mixed with fresh manure macroscopically and microscopically. Macroscopic examination was done by observing the condition of the skin that is discraped. Microscopic examination using native examination. Based on the negative examination, it was found that there were Sarcoptes sp. mites on an objective microscope with a 40x magnification. Geopelia striata can be infected with Sarcoptes sp. through direct contact with sick animals. The incubation period of Sarcoptes sp. after infection from infected animals was 3-6 weeks.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90409942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-31DOI: 10.20473/jmv.vol4.iss2.2021.189-192
Adam Fahmi Fiqih, S. Soeharsono, M. Yunita, Y. Dhamayanti, Aditya Yudhana
The aim of this study was examine the effect of robusta coffee in bali cattle semen. A total of six cattles cement were given four treatments each P0 without adding Robusta coffee extract, P1 was given an additional 5%, P2 10%, and P3 15%. The results are observed by assessing motility, viability, and abnormalities. The data obtained were analyzed by Analysis of Variance (ANOVA) and continued with the duncan. Based on the analysis of variance in obtaining the results of the study, it was shown motility of P0 was significantly different from P1, P2, and P3 (p<0.05). Viability of P0 (control) different significantly from P1 , P2, and P3 (p<0.05). P3 motility different significantly from P0, P1, and P2 (p<0.05). Addition of robusta coffee extract of 10% and 15% decreased spermatozoa quality.
{"title":"Toxic Effect of Robusta Coffee Extract (Coffea canephora) on Bali Cattle Spermatozoa After Equilibration","authors":"Adam Fahmi Fiqih, S. Soeharsono, M. Yunita, Y. Dhamayanti, Aditya Yudhana","doi":"10.20473/jmv.vol4.iss2.2021.189-192","DOIUrl":"https://doi.org/10.20473/jmv.vol4.iss2.2021.189-192","url":null,"abstract":"The aim of this study was examine the effect of robusta coffee in bali cattle semen. A total of six cattles cement were given four treatments each P0 without adding Robusta coffee extract, P1 was given an additional 5%, P2 10%, and P3 15%. The results are observed by assessing motility, viability, and abnormalities. The data obtained were analyzed by Analysis of Variance (ANOVA) and continued with the duncan. Based on the analysis of variance in obtaining the results of the study, it was shown motility of P0 was significantly different from P1, P2, and P3 (p<0.05). Viability of P0 (control) different significantly from P1 , P2, and P3 (p<0.05). P3 motility different significantly from P0, P1, and P2 (p<0.05). Addition of robusta coffee extract of 10% and 15% decreased spermatozoa quality.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"17 Suppl 5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80238308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-31DOI: 10.20473/jmv.vol4.iss2.2021.243-248
Trima Weliyani, I. S. Hamid, S. Hidanah, R. Prastiya, Prima Ayu Wibawati
Monosodium glutamate (MSG) is a sodium salt from glutamic acid which is currently very popular to be used as a food flavoring ingredient to stimulate appetite. Exessive consumption of MSG can disrupt the balance of antioxidants and ROS, and cause the negative effects of oxidative stress on testes. Coffee has high chlorogenic acid, function for antioxidants and reduce the negative effect on cell damage in the testes. This study aims to analyze the effect of robusta coffee extract on histological of mice testes induced with MSG. This study was completely randomized design (CRD), using 20 male mice for 5 treatment groups: K+ (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) orally for 42 days. Histophatological scores were analyzed with Saphiro-Wilk Test and Anova. There is no effect of increasing testicular weight. 0,12 mg MSG can cause a decrease in the number of spermatogonia and spermatocytes. 0,2 mg robusta coffee extract can maintain the number of spermatogonia and spermatocytes.
{"title":"Effect of Robusta Coffee Extract on Histopathological in Mice Testes Induced with Monosodium Glutamat","authors":"Trima Weliyani, I. S. Hamid, S. Hidanah, R. Prastiya, Prima Ayu Wibawati","doi":"10.20473/jmv.vol4.iss2.2021.243-248","DOIUrl":"https://doi.org/10.20473/jmv.vol4.iss2.2021.243-248","url":null,"abstract":"Monosodium glutamate (MSG) is a sodium salt from glutamic acid which is currently very popular to be used as a food flavoring ingredient to stimulate appetite. Exessive consumption of MSG can disrupt the balance of antioxidants and ROS, and cause the negative effects of oxidative stress on testes. Coffee has high chlorogenic acid, function for antioxidants and reduce the negative effect on cell damage in the testes. This study aims to analyze the effect of robusta coffee extract on histological of mice testes induced with MSG. This study was completely randomized design (CRD), using 20 male mice for 5 treatment groups: K+ (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) orally for 42 days. Histophatological scores were analyzed with Saphiro-Wilk Test and Anova. There is no effect of increasing testicular weight. 0,12 mg MSG can cause a decrease in the number of spermatogonia and spermatocytes. 0,2 mg robusta coffee extract can maintain the number of spermatogonia and spermatocytes.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74904942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-31DOI: 10.20473/jmv.vol4.iss2.2021.231-242
I. Wulandari, J. Rahmahani, Indah Rahmawati, Nurvita Putih, Aisyah Azahro, K. Kusnoto, R. Ernawati, F. Rantam
This study aimed to determine the mutation of amino acid, nucleotide homology, phylogenetic tree, epitope prediction of Hemagglutinin-Neuraminidase protein Newcastle Disease (ND) Virus isolated from traditional market around Surabaya. Samples were from 37 chicken with cloacal swab and one positive samples for control (LaSota). Samples were inoculated on embyonate chicken eggs and identified with HA test confirmed with HI test. Positive samples processed by PCR using forward and reverse primer with 503 bp RNA target. The PCR result then analyzed with sequencing. Result of sequencing analysis showed that theres similiarity between samples amino acid and vaccine isolate its effect the percentage of nucleotide homology and phylogenetic relation between isolate. Epitope NGAANNSGWGAPIHDPDYIGG have high immunogenic value at all of isolate which good as vaccine candidate.
{"title":"Molecular Characterization of Hemagglutinin-Neuraminidase Protein Virus Newcastle disease (ND) in Surabaya during 2003 and 2016","authors":"I. Wulandari, J. Rahmahani, Indah Rahmawati, Nurvita Putih, Aisyah Azahro, K. Kusnoto, R. Ernawati, F. Rantam","doi":"10.20473/jmv.vol4.iss2.2021.231-242","DOIUrl":"https://doi.org/10.20473/jmv.vol4.iss2.2021.231-242","url":null,"abstract":"This study aimed to determine the mutation of amino acid, nucleotide homology, phylogenetic tree, epitope prediction of Hemagglutinin-Neuraminidase protein Newcastle Disease (ND) Virus isolated from traditional market around Surabaya. Samples were from 37 chicken with cloacal swab and one positive samples for control (LaSota). Samples were inoculated on embyonate chicken eggs and identified with HA test confirmed with HI test. Positive samples processed by PCR using forward and reverse primer with 503 bp RNA target. The PCR result then analyzed with sequencing. Result of sequencing analysis showed that theres similiarity between samples amino acid and vaccine isolate its effect the percentage of nucleotide homology and phylogenetic relation between isolate. Epitope NGAANNSGWGAPIHDPDYIGG have high immunogenic value at all of isolate which good as vaccine candidate.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78160873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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