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Serological Study of Newcastle Disease in Ducks (Anas javanicus) Slaughtered in East Surabaya Traditional Market 东泗水传统市场屠宰鸭新城疫血清学研究
Pub Date : 2022-10-14 DOI: 10.20473/jmv.vol5.iss2.2022.131-137
Sellianova Ardhanella, R. Damayanti, S. Suwarno, F. Rantam, Kadek Rachmawati, A. Khairullah, J. Rahmahani
The purpose of this study was to determine the serological study of Newcastle Disease that infects slaughtered ducks (Anas javanicus) at the East Surabaya Traditional Market. This study was conducted from December 2018 to January 2019. A total of 122 samples were collected from four traditional markets in East Surabaya. The Haemagglutination Inhibition (HI) test was used to determine a positive result with an antibody titer of 4 or more. Samples for the HI test were collected from duck serum that had been treated with chicken red blood cells. The results showed 13 (10.65%) of the 122 samples were positive for ND.
本研究的目的是确定在东泗水传统市场屠宰鸭(Anas javanicus)感染新城疫的血清学研究。该研究于2018年12月至2019年1月进行。从东泗水四个传统市场共采集了122份样本。血凝抑制(HI)试验用于确定抗体滴度为4或以上的阳性结果。用于HI试验的样品是从用鸡红细胞处理过的鸭血清中收集的。结果122份样本中有13份(10.65%)呈ND阳性。
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引用次数: 0
Seroprevalence Brucellosis in Etawah Crossbreed goat in District Siliragung, Banyuwangi Banyuwangi Siliragung地区Etawah杂交山羊布鲁氏菌病血清阳性率
Pub Date : 2022-10-14 DOI: 10.20473/jmv.vol5.iss2.2022.138-144
A. L. Saputro, Ratih Novita Praja, Aditya Yudhana, Fauzan Mumtazi, Ma'rifatunnisa' Romadhona, A. Anastasya, Muhammad Riesta Farhan
Brucellosis is a zoonotic bacterial disease that is endemic in many countries, especially developing countries such as Indonesia. Livestock centers in Indonesia have not been free from Brucellosis disease, which reaches 40% and spread almost throughout Indonesia. This study aimed to detect the presence of Brucellosis antibodies in Etawah Crossbreed at Siliragung District, Banyuwangi using the Rose Bengal Test (RBT) method. The data that will be obtained later was expected to provide benefits in reducing the possibility of Brucellosis spread and can be used as a reference for Brucellosis control programs in goats. The results showed that the RBT examination in the sample population of Etawah Crossbreed in Siliragung District which was taken showed negative results in all samples. The conclusion of this study was that Brucella abortus antibodies were not found in Etawah Crossbreed in Siliragung District, Banyuwangi.
布鲁氏菌病是一种人畜共患细菌性疾病,在许多国家,特别是印度尼西亚等发展中国家流行。印度尼西亚的畜牧中心还没有摆脱布鲁氏菌病,其发病率达到40%,几乎在印度尼西亚全国蔓延。本研究旨在采用玫瑰孟加拉试验(RBT)方法检测班育旺吉省Siliragung区Etawah杂交品种中布鲁氏菌抗体的存在。预计以后将获得的数据将有助于减少布鲁氏菌病传播的可能性,并可作为山羊布鲁氏菌病控制规划的参考。结果表明,对西里拉贡地区埃塔瓦杂交品种样本种群的RBT检测结果均为阴性。本研究的结论是,在班育旺吉市西里拉贡区埃塔瓦杂交品种中未发现流产布鲁氏菌抗体。
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引用次数: 0
Surgical Procedure for Pyometra and Mammae Tumor Treatment in a Pitbull Dog 斗牛犬子宫脓肿及乳房肿瘤的外科治疗
Pub Date : 2022-04-22 DOI: 10.20473/jmv.vol5.iss1.2022.109-118
Nofan Rickyawan, Cheptien Winda Virgiantari, M. A. Lesmana, D. Vidiastuti
Pyometra is an infection or inflammation of the uterine wall characterized by the accumulation of pus in the uterine lumen. Predisposing factors include age, breed, bacterial infection, and hormones. Another disease that is often correlated with an imbalance in reproductive hormones is mammary tumors. A 7-year-old unspayed female Pitbull dog weighed 27.9 kg has clinical symptoms of frequent discharge of mucopurulent reddish-brown discharge with a foul odor from the vagina. The left mammary glands of the second, third, and fourth nipples have lumps about 20 cm in diameter, are solid in consistency, well-defined, the same color as the skin surface, and can be moved. Diagnosis performed on physical examination, hematology, blood chemistry, and cytology was pyometra and mammary tumors. Treatment was done by ovariohysterectomy and unilateral mastectomy. Post-surgery therapy was enrofloxacin 5 mg/kg, ketoprofen 2 mg/kg, phytomenadione 1 mg/kg, and Biodin® 0.1 ml/kg, followed by intensive surgical wound care. The patient recovered after two months of treatment.
脓膜炎是一种子宫壁感染或炎症,其特征是子宫腔内积液。诱发因素包括年龄、品种、细菌感染和激素。另一种常与生殖激素失衡相关的疾病是乳腺肿瘤。一只7岁未切除卵巢的雌性斗牛犬,体重27.9公斤,临床症状为经常从阴道排出粘液化脓性红褐色分泌物,并伴有恶臭。第二、三、四乳头左乳腺有直径约20厘米的肿块,稠度实,界限分明,与皮肤表面颜色相同,可移动。经体格检查、血液学、血液化学和细胞学诊断为子宫积脓和乳腺肿瘤。治疗采用卵巢子宫切除术和单侧乳房切除术。术后给予恩诺沙星5 mg/kg、酮洛芬2 mg/kg、植美二酮1 mg/kg、百奥定®0.1 ml/kg的治疗,并辅以外科伤口护理。病人经过两个月的治疗后康复了。
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引用次数: 3
Risk Factors and Scabious Prevalence in Cats During 2020 in Griya Satwa Clinic, Magetan 马吉坦格里亚萨特瓦诊所2020年猫疥疮流行的危险因素和流行程度
Pub Date : 2022-04-22 DOI: 10.20473/jmv.vol5.iss1.2022.81-86
Nedriana Cahya, H. Primarizky, M. Yunita
Scabious is a disease that often infests cats, originating from the family Sarcoptidae. This study aimed to determine the prevalence and factors that can affect the risk of scabious in cats. The study was evaluated at the Griya Satwa Clinic, Magetan in January-February 2021. The design of this study was a retrospective cohort study by taking secondary data during the 2020 period as study material. The data obtained were then entered into the Statistical Package for the Social Sciences (SPSS) version 20 for windows to analyze the association's relationship and increased risk by calculating the Chi-Square and Odds Ratio. The samples obtained were 76 cats infested with scabious. The analysis of the association of risk factors for scabious was breed (P = 0.428), age (P = 0.793), sex (P = 0.426). The results of the prevalence of scabious in cats from the study during January-February 2021 in the 2020 period were 9.5%, and the analysis of the association (Chi-Square) and the increased (Odds Ratio) of factors including breed, age, sex on the risk of scabious in cats were not significance / absent influence, The probability of error obtained is outside the tolerance set by the researcher, so the results are not significant (p>0,05).
疥疮是一种经常感染猫的疾病,起源于疥疮科。本研究旨在确定猫疥疮的患病率和影响风险的因素。该研究于2021年1月至2月在Magetan的Griya Satwa诊所进行了评估。本研究设计为回顾性队列研究,以2020年期间的二手数据为研究材料。然后将获得的数据输入社会科学统计软件包(SPSS) version 20 for windows,通过计算卡方和优势比来分析关联关系和增加的风险。获得的样本是76只感染疥疮的猫。结痂危险因素的相关分析为品种(P = 0.428)、年龄(P = 0.793)、性别(P = 0.426)。本研究2021年1月- 2月2020年期间猫疥疮患病率结果为9.5%,品种、年龄、性别等因素对猫疥疮风险的关联分析(χ 2)和增加(Odds Ratio)均无显著/不存在影响,得到的误差概率在研究者设定的容限范围之外,因此结果不显著(p>0,05)。
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引用次数: 1
The Association of Age and Number of Parturition with Mammary Gland Tumor Case Rate in Mice in Malang Raya 马郎拉雅地区小鼠乳腺肿瘤发病率与年龄、分娩次数的关系
Pub Date : 2022-04-22 DOI: 10.20473/jmv.vol5.iss1.2022.9-15
Maulidi Robingi Mardiyani Wukirani, Essly Hervianingsih Adha, Sang Ayu Putri Aristya Dewi, A. Setianingrum, D. A. Pratama, A. Hardian
Mammary gland tumours are frequently reported in adult female mice. It broadly affects the productivity and breeding performance of mice in breeding centres. The tumours might occur spontaneously due to internal factors such as genetic mutation and hormonal disturbance, however, external causes such as Mouse Mammary Tumour Virus (MMTV) infection was also reported. This study aimed to prove that age and number of parturition associate with mammary gland tumour case rate in female mice in Malang Raya. Chi-square tests were employed to analyse the association between risk factors and mammary gland tumour case rate in this study. Histopathologic analysis described three subtypes of mammary gland tumours in mice which were adenoma, adenocarcinoma type A and adenocarcinoma type B. Chi-square tests results of tumour case rate toward age and number of parturitions were consecutively 0,004 and 0,0025 which indicated that age and number of parturitions were associated with mammary gland tumours case rate in mice in Malang Raya. The mapping of tumour case distribution visually showed a trend that higher disease proportions were spotted in higher altitude of sampling location. This might relate to the geographical breeding characteristics or temperature which needs further study to explore the association of spatial factors with the mammary tumour incidence in mice.
乳腺肿瘤在成年雌性小鼠中经常被报道。它广泛影响育种中心小鼠的生产力和繁殖性能。肿瘤可能是由于基因突变和激素紊乱等内部因素自发发生的,但也有报道称外部原因如小鼠乳腺肿瘤病毒(MMTV)感染。本研究旨在证明年龄和分娩次数与玛琅拉雅地区雌性小鼠乳腺肿瘤发病率的关系。本研究采用卡方检验分析危险因素与乳腺肿瘤发病率之间的关系。组织病理学分析发现,小鼠乳腺肿瘤有腺瘤、腺癌A型和腺癌b型三种亚型。肿瘤发病率对年龄和分娩次数的卡方检验结果分别为0,004和0,0025,表明年龄和分娩次数与玛朗拉雅地区小鼠乳腺肿瘤发病率相关。肿瘤病例分布图直观地显示了采样地点海拔越高,发病率越高的趋势。这可能与地理育种特征或温度有关,需要进一步研究空间因素与小鼠乳腺肿瘤发病率的关系。
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引用次数: 0
An Efficacy of Seligi Leaf Flour Fermentation on Cholesterol Levels, Low Density Lipoprotein, and High Density Lipoprotein in Catfish Seligi叶粉发酵对鲶鱼胆固醇、低密度脂蛋白和高密度脂蛋白水平的影响
Pub Date : 2022-04-22 DOI: 10.20473/jmv.vol5.iss1.2022.41-47
Reza Hardiansyah, M. Lamid
Catfish is a freshwater fish with a high economic value, so it is very good to be developed. Feed is one of the most important components in the cultivation process because it plays a role in supporting the plants and the survival of cultivated fish. Seligi (Phyllanthus buxifolius) is a native Indonesian medicinal plant that has been widely used for medicinal purposes. Seligi leaf extract is known to contain flavonoids, polyphenols (tannins), saponins, alkaloids, quinones, and triterpenoid steroids. The purpose of this research was to determine the effect of using fermented seligi leaf flour in feed to reduce levels of total cholesterol, low-density lipoprotein, and high densities lipoprotein in catfish meat. The method used in this research is the experimental method. The main parameters in this study were total cholesterol, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) in catfish meat. The supporting parameters in this research are water quality which includes temperature, pH, dissolved oxygen (DO), and ammonia (NH3-N). The results showed that the total cholesterol content of catfish meat ranged from 116.84-233.65 mg/dL. Meanwhile, the results of LDL in catfish meat ranged from 37.89-74.22 mg/dL. Then, the content of HDL in catfish meat ranges from 89.25-225.87 mg/dL.
鲶鱼是一种经济价值很高的淡水鱼,具有很好的开发前景。饲料是养殖过程中最重要的组成部分之一,因为它在支持植物和养殖鱼类的生存中起着重要作用。毛竹(Phyllanthus buxifolius)是一种印度尼西亚本土药用植物,已被广泛用于药用目的。已知Seligi叶提取物含有黄酮类化合物、多酚(单宁)、皂苷、生物碱、醌类和三萜类固醇。本研究的目的是确定饲料中添加发酵seligi叶粉对降低鲶鱼肉中总胆固醇、低密度脂蛋白和高密度脂蛋白水平的影响。本研究采用的方法是实验法。本研究的主要参数是鲶鱼肉中的总胆固醇、低密度脂蛋白(LDL)和高密度脂蛋白(HDL)。本研究的支持参数为水质,包括温度、pH、溶解氧(DO)和氨(NH3-N)。结果表明,鲶鱼肉的总胆固醇含量为116.84 ~ 233.65 mg/dL。同时,鲶鱼肉中LDL含量在37.89 ~ 74.22 mg/dL之间。结果表明,鲶鱼肉中HDL含量为89.25 ~ 225.87 mg/dL。
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引用次数: 2
Immunogenicity of Local and Massachusetts Strains Infectious Bronchitis Virus 传染性支气管炎本地和麻州毒株的免疫原性
Pub Date : 2022-04-22 DOI: 10.20473/jmv.vol5.iss1.2022.98-102
J. Rahmahani, R. Z. F. A. M. Wudhu, S. Suwarno, Martia Rani Tacharina
This study aimed to compare the immunogenicity between the whole virus and protein S of both local strain I-147 and Massachusetts strain of Infectious bronchitis virus with indirect ELISA. 24 healthy mice are divided into four groups with six mice in each group that are: P1) a group of mice that are vaccinated with S protein (spike glycoprotein) of local strain Infectious Bronchitis virus; P2) a group of mice that are vaccinated with S protein (spike glycoprotein) of Massachusetts strain Infectious Bronchitis virus; P3) a group of mice that are vaccinated with the whole virus of local strain Infectious Bronchitis virus; P4) a group of mice that are vaccinated with the whole virus of Massachusetts strain Infectious Bronchitis virus. The results of this research are Optical Density (OD) values of each treatment, then to be analyzed using One Way ANOVA and followed by Duncan Multiple Range Test 5% and T-test. The results show that there are differences in immunogenicity between the whole virus and S protein in both the local strain and Massachusetts strain of Infectious bronchitis virus. The OD value of the whole virus in the two strains has a significant difference from the OD value of the protein S antigen in both strains, both in the serum taken at week 2 and week 4 post-vaccination.
本研究旨在用间接ELISA法比较传染性支气管炎本地株I-147和马萨诸塞州株全病毒和蛋白S的免疫原性。将24只健康小鼠分为4组,每组6只,分别为:P1)接种本地株传染性支气管炎病毒S蛋白(刺突糖蛋白)的小鼠组;P2)接种马萨诸塞州株传染性支气管炎病毒S蛋白(刺突糖蛋白)的小鼠组;P3)接种本地株传染性支气管炎病毒全病毒的小鼠组;P4)一组小鼠接种了马萨诸塞州传染性支气管炎病毒株的全病毒。本研究结果为各处理的光密度(OD)值,采用单因素方差分析(One - Way ANOVA)进行分析,并采用Duncan多元极差检验5%和t检验。结果表明,传染性支气管炎本地株和麻州株的全病毒和S蛋白的免疫原性存在差异。接种后第2周和第4周血清中两株全病毒OD值与两株蛋白S抗原OD值存在显著差异。
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引用次数: 0
The Risk Factor of Subclinical Mastitis Incident in Dairy Cattle in KPSP Ijen Makmur, Banyuwangi Banyuwangi, Ijen Makmur KPSP奶牛亚临床乳腺炎事件的危险因素
Pub Date : 2022-04-22 DOI: 10.20473/jmv.vol5.iss1.2022.48-53
Indah Puspita Ningrum, S. Soeharsono, Prima Ayu Wibawati, Y. Dhamayanti, M. Yunita
Mastitis is an inflammatory disease in the internal tissue of the mammary gland. Subclinical mastitis does not show any changes in the shape of the mammary gland and to detect it can be examined the number of somatic cells in milk. Subclinical mastitis uses the California Mastitis Test (CMT) method as preliminary research to obtain preliminary data on the incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency. This research is descriptive research. The detection for this research was carried out by taking milk samples in cows per purposive and reacted CMT reagents issued circularly for 10-15 seconds. Observation of results is determined based on changes inconsistency with the existence of gel and the results of interview farmers as supporting data. The results showed that the influencing factors incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency were milk production, age and amount of lactation. In contrast, the biggest factor for reducing the risk of subclinical mastitis is udder health followed by body conditions measured by Body Condition Score.
乳腺炎是乳腺内部组织的一种炎症性疾病。亚临床乳腺炎不表现出乳腺形状的任何变化,可以通过检查乳汁中体细胞的数量来检测。亚临床乳腺炎采用加州乳腺炎试验(California mastitis Test, CMT)方法作为初步研究,获得Banyuwangi reggency Licin区KPSP Ijen Makmur亚临床乳腺炎发病率的初步数据。本研究为描述性研究。本研究的检测是通过在奶牛中采集牛奶样本,每个目的和反应的CMT试剂循环发放10-15秒。观察结果以与凝胶存在不一致的变化为依据,以农户访谈结果为支持数据。结果表明,班育旺吉县利津县伊仁Makmur县KPSP亚临床乳腺炎发病率的影响因素为产奶量、年龄和泌乳量。相比之下,降低亚临床乳腺炎风险的最大因素是乳房健康,其次是身体状况评分。
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引用次数: 0
Probiotic Potential of Lactic Acid Bacteria on Feed Efficiency, Weight and Carcass Percentage in Ducks 乳酸菌益生菌对鸭饲料效率、体重和胴体率的影响
Pub Date : 2022-04-22 DOI: 10.20473/jmv.vol5.iss1.2022.69-73
Evan Chandra, W. Lokapirnasari, S. Hidanah, M. A. Al-Arif, W. Yuniarti, E. Luqman
The aim of the study was to determine the effect of the use of probiotic lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, and Bifidobacterium sp) on feed efficiency, carcass weight and carcass percentage in broiler ducks. Probiotics lactic acid bacteria produce bacteriocins and organic acids that inhibit the growth of pathogenic microbes in the digestive tract of poultry, so that the intestinal villi of broiler ducks are healthy. Besides that, probiotics are able to reduce cholesterol levels in meat when consumed by humans and can improve health. This study used 48 peking ducks. The probiotics of Lactic Acid Bacteria used were a concentration of 1.2 x 108 CFU/ml. In this study there were four different treatments, namely P0 (without probiotics), P1 (given 1 ml probiotics/liter drinking water), P2 (given 2 ml probiotics/liter drinking water) and P3 (given 3 ml probiotics/liter of drinking water). The results of the feed efficiency study showed that there was a significant difference (p<0.05) in the P0, P1, P2 and P3 treatments. The highest feed efficiency was at P3 (33.81%) while the lowest was P0 (30.84%). The results of the study on carcass weight indicated that the probiotics tended to be higher than those who were not given probiotics but statistically not significantly different (p>0.05). The highest carcass weight was P3 (1749.92 g) and the lowest was P0 (1709.25 g). The results of the study on the percentage of carcass weight showed a significant difference (p<0.05) in both P0, P1, P2 and P3 treatments. The highest percentage of carcass weight was at P3 (64.60%) and the lowest was P0 (60.96%). It can be concluded that the administration of probiotics lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, and Bifidobacterium sp) at a dose of 1 ml/liter of drinking water, 2 ml/liter of drinking water and 3 ml/liter of drinking water can affect feed efficiency and the percentage of duck carcasses. but has no effect on the carcass weight of broiler ducks.
本试验旨在研究益生菌乳酸菌(嗜酸乳杆菌、干酪乳杆菌、乳酸乳球菌和双歧杆菌)对肉仔鸭饲料效率、胴体重和胴体率的影响。益生菌乳酸菌产生细菌素和有机酸,抑制家禽消化道内病原微生物的生长,使肉鸭肠绒毛健康。除此之外,益生菌还能降低人体食用肉类时的胆固醇水平,促进健康。本研究以48只北京烤鸭为研究对象。乳酸菌使用的益生菌浓度为1.2 × 108 CFU/ml。在本研究中,有四种不同的处理,即P0(不含益生菌)、P1(每升饮用水给予1 ml益生菌)、P2(每升饮用水给予2 ml益生菌)和P3(每升饮用水给予3 ml益生菌)。饲料效率研究结果表明,两者之间存在显著差异(p0.05)。胴体质量最高的是P3 (1749.92 g),最低的是P0 (1709.25 g)。研究结果表明,P0、P1、P2和P3处理的胴体质量百分比差异显著(p<0.05)。P3期胴体重比例最高(64.60%),P0期最低(60.96%)。由此可见,饲粮中添加1 ml/l、2 ml/l和3 ml/l饮水剂量的益生菌乳酸菌(嗜酸乳杆菌、干酪乳杆菌、乳酸乳球菌和双歧杆菌)可影响饲料效率和鸭尸率。但对肉鸭胴体重无影响。
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引用次数: 1
Betanodavirus Infection in Barramundi in Riau Islands 廖内群岛鲈鱼感染倍体虫病毒
Pub Date : 2022-04-22 DOI: 10.20473/jmv.vol5.iss1.2022.34-40
Achmad Rifai, T. Wulandari, Desi Surastini, Pramudya Dwi Wahyu Irawanto
Diseases that can cause very high economic losses are viruses and one of them is Betanodavirus or commonly called Viral Nervous Necrosis (VNN). VNN is a disease registered by The Office International des Epizooties (OIE) and is a major problem in marine fishery production in the world. This virus is able to infect fish from larva size to consumption size in a short period of time to reach death with a frequency of 80% to 100%. The disease can infect barramundi and is spread throughout the Riau Islands. This study used a live fish sampling method showing VNN signs and freshly dead fish. The research samples were obtained from several cities and districts in the Riau Islands Province, including Batam City, Bintan Regency, and Tanjung Balai Karimun Regency, the sample selection was based on clinical symptoms and fish mortality. Then the samples were necropsied for eye and brain organs, then amplified using primers targeting the RNA 2 gene. The results showed that VNN had been detected in the Riau Islands Province, i.e. Batam City, Bintan Regency, and Tanjung Balai Karimun Regency.
能造成巨大经济损失的疾病是病毒,其中一种是倍达诺病毒或通常称为病毒性神经坏死(VNN)。VNN是国际兽疫局(OIE)登记的一种疾病,是世界海洋渔业生产中的一个主要问题。该病毒可在短时间内感染鱼类,从幼虫大小到食用大小,死亡率为80%至100%。这种疾病可以感染澳洲undi,并在廖内群岛蔓延。本研究采用活鱼采样方法,显示VNN信号和新鲜死鱼。研究样本来自廖内群岛省的几个城市和地区,包括巴淡市、民丹县和丹绒巴莱卡里蒙县,样本选择基于临床症状和鱼类死亡率。然后对样本进行尸检,寻找眼睛和大脑器官,然后使用针对RNA 2基因的引物进行扩增。结果显示,在廖内群岛省,即巴淡市、民丹县和丹绒巴莱卡里蒙县均发现了VNN。
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引用次数: 2
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Jurnal Medik Veteriner
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