Lasers in Medical Science最新文献

The aim of this study was to evaluate the effectiveness of an aquatic progressive resistance exercise (APRE) and PBM (associated or not) on morphology of skeletal muscle and biochemical markers using an experimental model of knee osteoarthritis (OA). Fifty male Wistar rats were randomly distributed into 5 groups: control group (CG); OA control (OAC); OA submitted to APRE (OAE); OA submitted to PBM (OAL); OA submitted to APRE and PBM (OAEL). Trained rats performed a water-jumping program carrying a load equivalent to 50-80% of their body mass strapped to their chest. Laser irradiation (808 nm) was performed on 2 points of the knee joint. Treatments (3 days a week, for 8 weeks) started 4 weeks after the OA induction. The results showed that all OA groups presented a significantly increase in the muscle cross-section area (CSA) and a decrease in muscle fiber density compared to CG. Moreover, both trained groups presented a reduced expression of atrogin and an intense myoD immunoexpression in the laser exercised animals. The results demonstrate that APRE was effective in reducing muscle atrophy markers and its association with PBM could be effective in modulating molecules involved in muscle recovery in knee OA.

To evaluate the quality, usefulness, and reliability of videos about Small Incision Lenticule Extraction (SMILE) surgery on the YouTube platform. On January 19, 2022, a search was performed on YouTube ( www.youtube.com ) with the keywords 'small incision lenticule extraction', 'SMILE refractive surgery', and 'ReLex SMILE Surgery'. The default search option was "sort videos by relevance". The duration of videos (seconds), the number of views, the source of videos (surgeons/medical organizations-health channels/patients-others), the number of subscribers, the number of likes and dislikes, like ratio (like × 100/[like + dislike]), the number of comments, the time since upload date (days), the video content (surgery/theoretical information), the content of surgical videos (real surgery/animation), mode of expression (verbal narration/subtitle), and the presence of a conflict of interest (yes/no) were recorded. The videos were blindly evaluated by two refractive surgeons (SA and AK) using DISCERN, the Journal of the American Medical Association (JAMA), and the Global Quality Score (GQS). A total of 101 videos were analyzed, 38 (37.6%) of which were uploaded by surgeons. The mean DISCERN, JAMA, and GQS were 37.73 ± 7.49; 1.90 ± 0.57; and 2.20 ± 0.73, respectively. The JAMA score, GQS, and video duration were all significantly correlated with the DISCERN score. The GQS was significantly correlated with all parameters except the JAMA score. Overall, SMILE surgery videos on the YouTube platform may provide cursory information to non-ophthalmologists; however, when the videos are evaluated using tools such as DISCERN, JAMA, and GQS, they are of low quality in terms of refractive surgery education for ophthalmologists. Experts should evaluate and review content uploaded to websites such as YouTube.

This study aimed to assess and compare the effectiveness of adding low-level laser therapy (LLLT) and neuromuscular electrical nerve stimulation (NMES) to conventional physical therapy exercises, for stroke patients with hemiplegic shoulder pain (HSP). Seventy-five stroke patients with shoulder pain were included in this prospective randomized controlled study. Participants were divided into three groups. All patients underwent a multidisciplinary rehabilitation program five days a week for four weeks for a total of twenty sessions with classical physical therapy exercises. In addition, Group 1 received LLLT for three days a week for four weeks. Group 2 received NMES for twenty minutes for five days a week for four weeks. Group 3 control group received classical physical therapy exercises. Brunnstrom (BRS) upper extremity, BRS Hand, Barthel index, Shoulder Pain and Disability Index (SPADI), Fugl Meyer, Modified Ashworth Scale (MAS) and visual analog scale (VAS) were assessed, prior to the treatment and at the end of four weeks. After treatment, statistically significant improvements were found in BRS upper extremity, BRS Hand, Barthel index, SPADI, Fugl Meyer and VAS in all three groups (all p < 0.005). When the groups were compared, significant improvements in Bartel, SPADI and VAS in the LLLT and NMES groups than the control group(all p < 0.005), however the LLLT and NMES groups were statistically similar. LLLT, NMES and conventional exercise therapy have demonstrated efficacy in treating HSP and improving upper extremity functions and disability. Laser and NMES were more effective while the effects of laser and NMES were similar.

This systematic review and meta-analysis aimed to compare the effect of photobiomodulation (PBM) therapy on implant stability and crestal bone loss placed in healed sites. The present systematic review and meta-analysis were conducted according to PRISMA (The Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. Two investigators carried out the electronic search of Pubmed, Google Scholar, and Ebscohost for published literature from 2012 till March 2024. Handsearch for articles relevant to the topic was also carried out along with references. Only randomized controlled trials were included for systematic review. The assessment quality was done using the revised JBI checklist (Joanna Biggs Institute). After screening and eligibility assessment, Qualitative analysis was envisaged for 15 studies on implant stability and crestal bone loss, followed by quantitative analysis of 8 studies on implant stability and only 2 studies for crestal bone loss. Meta-analysis showed that overall implant stability was significantly higher in the control group (P < 0.00001). Crestal bone loss showed a significant difference after the therapy when observed after six months of implant placement (P = 0.03). Heterogeneity was high with the random effects model for implant stability, while it was acceptable for crestal bone loss. However, more studies with similar parameters concerning implant stability and PBM characteristics are required for further subgroup analysis and to reach conclusive results.

In this article, we aim to evaluate the effects of photobiomodulation on mitochondria quantity, biogenesis, and mitophagy-associated genes in breast cancer (BC) cells. Both models were irradiated with a low-power infrared laser (880 nm, 150 mW) and amber LED (617 nm, 1500 mW), alone or simultaneously. We evaluated the mRNA expression of PINK1 and PGC-1α genes, and the mitochondrial number was assessed based on the ratio of mitochondrial DNA/genomic DNA (mtDNA/gDNA). No significant difference was observed in the mtDNA/gDNA ratio comparing the low-power infrared laser (LPIL) and LED-irradiated groups to their control counterparts. Similarly, no difference was observed in the mRNA levels of PINK1 and PGC-1α of the irradiated group with an LPIL and LED alone or in combination. In conclusion, PBM with LPIL and LED did not alter the mtDNA/gDNA ratio nor modulate the mRNA levels of the genes related to mitophagy and biogenesis in BC cells.

Hemangiomas are common benign vascular tumors that often present in childhood. The 595 nm pulsed dye laser (PDL) is now being widely used to treat vascular skin lesions. This case series was performed to review the therapeutic efficacy and safety of PDL in the treatment of hemangiomas among Vietnamese patients. A total of 43 Vietnamese patients (79.1% females), mean age was 8.83 years old (2 months - 54 years old), with a clinical diagnosis of hemangioma, were treated with the PDL over 2 years. Treatments were performed every 4 weeks. Hemangioma lesions that occur on the head and the neck are seen in 65.1%, 67.4% of superficial, and 32.6% of mixed hemangioma subtypes. The mean treatment is 5.3 times. Excellent-good results were achieved in 86.1%, average results in 11.6%. No permanent side effects during the treatments were observed. Treatment of hemangiomas using the 595 nm PDL is an effective and safe method in the skin types of Vietnamese patients. The effect does require several treatment sessions.

The aesthetic understanding has found its place in dental clinics and prosthetic dental treatment. Determining the appropriate prosthetic tooth color between the clinician, patient and technician is a difficult process due to metamerism. Metamerism, known as the different perception of the color of an object under different light sources, is caused by the lighting differences between the laboratory and the dental clinic. The traditional trial-error color determination method, coupled with the high cost of instrumental color value determination, has prompted the need for alternative technologies. The integration of AI technologies into dental practices aims to minimize errors in tooth shade assessment, reduce equipment usage, eliminate the impact of clinic lighting on color detection, and decrease costs for patients, dentists, and laboratories. In this study, a machine learning (ML) based approach that can correctly detect tooth shade even under different clinical lights has been developed. A dataset consisting of 580 dental images taken under four different clinical lights and with five repetitions was created using the Vita color shade guide. Experimental studies were performed using the HSV color space, 6 different ML algorithms and color histograms. As a result, 97.93% accuracy rate was achieved by using cross-validation (cv = 5) in the classification of 29 color values ​​independent of clinical lights. It has been shown that the tooth colors can be determined with high accuracy using ML algorithms and metamerism can be prevented.

This systematic review aimed to compare postoperative pain in endodontic treatments using PIPS Er: YAG laser-activated irrigation (LAI) versus conventional needle irrigation. An electronic search was conducted to identify randomized clinical trials (RCT) investigating postoperative pain in patients who underwent root canal treatments in permanent teeth using PIPS Er: YAG laser-activated irrigation or conventional needle irrigation. Two reviewers performed study selection, data extraction, risk of bias assessment (RoB 2.0 tool), and the certainty of evidence (GRADE). The meta-analyses were conducted using Review Manager software (p ≤ 0.05). The mean difference (MD) was chosen as the effect measure, and a random-effect model was employed, along with a 95% confidence interval (CI). The search identified 2864 records, and after selecting, three RCTs were included in the quantitative analysis. The meta-analysis showed a difference in postoperative pain according to the evaluation time. The subgroup meta-analysis revealed that the PIPS Er:YAG showed a significant reduction in the postoperative pain in the 48 hours (MD = -0.78; 95% CI [-1.39, -0.17]; p = 0.01; I² = 69%) compared to the control group. However, no statiscally significant results were found when assessing postoperative pain after 1, 3 and 7 days (95% CI; p > 0.05). The PIPS Er: YAG laser-activated irrigation reduced postoperative pain at 2 days following primary endodontic treatment. However, the certainty of the evidence is low and further RCTs are needed to confirm these results and avoid bias and confounding factors. Registration: PROSPERO registration number: CRD42023432499.

Skin flap transplantation is a conventional wound repair method in plastic and reconstructive surgery, but infection and ischemia are common complications. Photobiomodulation (PBM) therapy has shown promise for various medical problems, including wound repair processes, due to its capability to accelerate angiogenesis and relieve inflammation. This study investigated the effect of red and blue light on the survival of random skin flaps in methicillin-resistant Staphylococcus aureus (MRSA)-infected Sprague Dawley (SD) rats. Forty male SD rats were divided into control and light-emitting diode-red and blue light-treated (LED-RBL) groups at a ratio of 1:1 and a McFarland flap procedure was performed, which was subsequently infected with MRSA strains. After 7 days, the appearance and survival of the flaps were evaluated. The microvascular density was determined by hematoxylin and eosin (HE) staining. The expression levels of vascular endothelial growth factor (VEGF), hypoxia inducible factor 1α (HIF-1α), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (normally expressed as AKT) were detected by immunohistochemistry. The flap survival rate and microvascular density in the LED-RBL group were significantly higher than those in the control group (P < 0.05). In addition, the VEGF, HIF1-α, PI3K, and AKT levels were significantly higher in the LED-RBL group compared to the control group (P < 0.05). Red and blue light increased the survival area of the infected flap in rats by promoting angiogenesis, relieving oxidative stress, and reducing bacterial loads, indicating that PBM therapy is a convenient, simple, analgesic, and safe treatment intervention in promoting the survival rate of transplanted flaps after wound repair surgery.