This study aimed to assess the impact of nutritional conditions and irradiation parameters on the viability and proliferation of murine preosteoblasts. MC3T3-E1 cells were maintained under standard culture conditions (αMEM supplemented with 10% fetal bovine serum) or nutritional deficit conditions (αMEM without serum) and irradiated or not (control) with an InGaAlP diode laser at wavelengths of 660 nm (red) or 790 nm (infrared), with doses of 1, 4, or 6 J/cm², in a single dose in continuous mode. Cell viability and proliferation were assessed 24, 48, and 72 h after irradiation using the Alamar blue reduction assay. The cell cycle and events related to cell death were evaluated via propidium iodide (PI) staining and Annexin V/PI assays, respectively, through flow cytometry. The data revealed that in cells cultured with normal nutrition (10% FBS), there was no significant difference (p > 0.05) in cell viability or proliferation among the different irradiation protocols. In contrast, in the experiments conducted under nutritional deficiency, the infrared laser at a dose of 6 J/cm² significantly increased (p < 0.05) cell viability and proliferation compared with those of the control group at 72 h. The data were confirmed by cell cycle and cell death events (Annexin V/PI) assays. These results suggest that in vitro PBM yields more consistent biostimulatory effects on pre-osteoblasts subjected to nutritional deficiency, highlighting the need for attention to simulate these conditions in studies with laser therapy in in vitro bone disease models and in in vitro experiments using PBM for bone tissue engineering.
Bell's palsy, characterized by sudden facial paralysis, affects a significant proportion of the population annually, yet its exact cause remains elusive. Recently, increasing attention has been directed towards photobiomodulation therapy (PBMT) and its prospective contribution to managing various neurological and musculoskeletal conditions, including Bell's palsy. A systematic literature search of different databases was performed using a specific search strategy to find eligible studies that evaluated the potential of PBMT in managing Bell's palsy, considering its non-invasive nature and tissue healing properties. Data extraction was done based on inclusion and exclusion criteria. The systematic review included studies published from 1993 to 2022, examining the effects of photobiomodulation therapy on Bell's palsy. The studies, involving 21 to 120 participants aged 18 to 70, utilized various PBMT parameters such as wavelengths (830-850 nm and 808/905 nm), energy densities (8-20 J/cm²), and irradiation times (10-125 s). The results demonstrated significant improvements in facial muscle function, pain intensity, and muscle strength, showing that PBMT is effective in enhancing facial nerve function recovery in Bell's palsy patients. Statistical analysis showed that PBMT treatments had significant improvements compared to control groups (p < 0.05). Our systematic review indicates that photobiomodulation therapy can improve facial muscle function, pain intensity, and muscle strength in Bell's palsy patients. Notably, wavelengths of 830-850 nm and the combination of 808/905 nm yielded the most favorable results. However, due to heterogeneity in study designs, PBMT parameters, and outcome measures, further standardized and well-designed randomized controlled trials are necessary to establish optimal treatment protocols and validate these findings comprehensively.
Recently, a minimally invasive procedure based on a laser technique (SiLaT) has been developed for the treatment of pilonidal cysts. Although less invasive and less painful than surgery, this solution is nevertheless limited by its high cost. Other more affordable laser devices, such as the holmium laser, are also used in minimally invasive surgery. The objective of this study was to evaluate the possibility of using the holmium laser instead of the SiLaT laser in the treatment of pilonidal cysts. Retrospective cohort study with the primary endpoint being the cure rate one month after treatment. Median duration of local care was 21 days (mean = 22 ± 7.5) and healing rate at 1 month was 90.7%. During follow-up, 102 patients (44.9%) experienced pain in the coccygeal region often exacerbated by sitting and significantly more common in people with a small frame, overwhelmingly female. A surgical site infection was reported in 36 patients (15.9%). Recurrence, occurred in 39 patients (17.2%), was related to cyst type (type 1 do not recur, type 3 recur twice three time than type 2). Holmium laser does not differ from SiLaT laser in the healing rate of pilonidal cysts after treatment. However, it is characterised by a moderately higher incidence of complications, foremost of which is the occurrence of pain that can persist for up to a year after the procedure and which could be related to an increase in heat inherent to the use of holmium. As a result, this procedure does not seem to represent an alternative to SiLaT.
Traditional tooth preparation can cause patient discomfort, thermal damage to tissues, and occupational health risks for clinicians. Laser-based techniques, particularly femtosecond lasers, offer an alternative due to precise, non-invasive treatment without the thermal and mechanical drawbacks. The objective of this study was to assess available evidence on the effects of femtosecond laser treatment on enamel and dentin. The study design included in vitro or in vivo studies on human teeth reporting on qualitative and quantitative parameters of laser-dental tissue interaction. The review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) statement. The review was registered in the Open Science Framework registry. A comprehensive literature search of PubMed, Scopus, EMBASE/Ovid, Cochrane Library, DARE, LILACS and Pro Quest databases was conducted by three reviewers until 10th January 2024 and then updated on 18th August 2024. Eligibility criteria included peer-reviewed articles published in English, focusing on human teeth, with available full text excluding reviews, conference proceedings and gray literature. The outcomes of interest were the quality of tooth preparation, surface characteristics, bond strength, thermal effects and damage to adjacent tissues with variables being laser parameters and tooth types. Methodological quality of studies was not conducted. The search yielded 48 articles after the removal of duplicates, irrelevant and non-retrievable articles. All were original in vitro studies investigating reporting on the outcomes of interest. Measurement methods included a range of microscopy, spectroscopy, optical coherence tomography, universal force testing and thermal imaging. Sapphire lasers were most commonly used with a wide range of parameters. Laser produced sharp-edged cavities with ablated surfaces free of melting (seen in approximately 10% of the craters), cracking, debris, with open dentinal tubules, increased wetting (Contact angle mean range 39.63 ̊(± 1.84 ̊) to 70.47 ̊(± 10.27 ̊) and roughness mean range 0.82 (± 0.05) to 4.20 (± 1.10)µm). Effects of femtosecond laser on bonding efficiency were inconsistent when compared to no treatment, conventional acid etching or Er:YAG laser. Femtosecond laser was shown to achieve precise tooth preparation with no or minimal thermal, mechanical and structural effects on adjacent tissue, however with variable bonding efficiency. Further research is needed to optimize laser parameters, investigate antibacterial effects, and establish long-term clinical outcomes and safety profiles.Date of registration: July 1st, 2024.Registration ID: OSF.IO/UQMNB.
After dental extractions, patients may experience post-operative pain, with varying degree of severity among patients. The use of Er, Cr: YSGG laser in oral wound healing has garnered considerable interest and attention in recent years. The present study aims to evaluate and assess the wound healing and post operative pain after extraction of teeth in pediatric patients with application of Er, Cr: YSGG laser. Children aged 7-14 years of age with alteast one grossly decayed permanent molar indicated for extraction were included in the study. 60 teeth were randomly divided in two group- one receiving conventional treatment with post extraction pressure pack gauze and one receiving Er, Cr: YSGG laser bandage treatment. A post operative assessment was done by WBFPRS and Wound healing index for post operative pain and wound healing respectively on 24 h, 3rd, 7th and 14th day follow up. Er, Cr: YSGG laser bandage group showed statistically significant difference in both post operative pain and wound healing on follow ups. The study shows that application of laser bandage can be done in post operative extraction sockets to alleviate wound healing, decreasing post operative pain and discomfort.
Recurrent aphthous stomatitis (RAS) is a common oral lesion with no definitive treatment; current therapies primarily focus on symptom relief. This study aims to address the lack of high-quality clinical research by evaluating the effectiveness of Photobiomodulation Therapy (PBMT) using GaAlAs 808 nm, AlGaInp 660 nm, and GaAs 635 nm lasers for managing aphthous lesions. The study focuses on the lasers' effectiveness in reducing pain, accelerating healing, enhancing patient satisfaction, and preventing recurrence compared to a control group. A randomized controlled clinical study was conducted with 64 RAS patients, divided into four groups: 808 nm laser, 660 nm laser, 635 nm laser, and a placebo. Pain severity, ulcer size, and erythema were evaluated at various time points before, immediately after, and several days post-treatment, while patient satisfaction and recurrence were assessed after one month. All laser groups significantly reduced pain intensity, ulcer size, and erythema, with the 808 nm laser showing the most pronounced effects. Mean pain scores in the laser groups decreased to zero by day 7, whereas the control group had a slower reduction (p < 0.001). Ulcer size was significantly smaller in the 808 nm group on days 3 and 7 (p < 0.05). Erythema was significantly reduced by day 7 (p < 0.05). The 808 nm laser also resulted in the highest patient satisfaction, with no recurrences observed in any group. PBMT is an effective treatment for RAS, and the 808 nm laser was the most effective wavelength used.
Aim: The aim of this study was to evaluate the expression levels of vascular endothelial growth factor (VEGF), Peroxiredoxin 1 (PRX1), glucose transporter 1 (GLUT1) and type I collagen (COL1) and the rate of tooth movement comparing 3 accelerated tooth movement (ATM) methods: Corticopuncture (CP), photobiomodulation (PBM) and the combined technique (CP + PBM) on days 1, 3, 7 and 14.
Methods: Orthodontic tooth movement was induced in 24 male Wistar rats. CP procedure included three perforations: two in the palate and one mesial to the molars. GaAlAs diode laser irradiation was performed on days 0, 2, 4 and 6, totaling 4 irradiations. 14 days (810 nm, 100 mW, 15 s). Gingival tissue was collected from the cervical area of both first molars and qPCR was performed to isolate and quantify mRNA levels.
Results: All ATM groups showed increased tooth displacement compared to control after 14 days (20% for PBM; 40% for CP and 60% for CP + PBM). PBM showed higher VEGF expression on days 1,3 and 7 followed by CP and CP + PBM. PRX1 levels increased on days 1 and 3 in PBM and CP + PBM. GLUT1 increased on day 3 in all groups. No difference was found on levels of VEGF, PRX1 and GLUT1 among the groups on day 14, except for COL1 which increased significantly in PBM group.
Conclusion: All ATM methods showed higher expression of all of VEGF, PRX1, GLUT1, COL1 than control group. PBM and CP + PBM groups had more expression related to angiogenesis, glucose uptake, oxidative stress and collagen synthesis.
This study aimed to investigate the influence of photobiomodulation therapy (PBMT) in hypoxia (HYP) and normoxia (NOR) on time to exhaustion and the energetic contribution of treadmill running in amateur athletes. Eleven male participants, aged between 18 and 29 years, were submitted to an incremental test to determine the iV̇O2max, and subsequently on separated days, to the PBMT application (irradiation = 720 s; wavelength = 850 nm), followed by four efforts to exhaustion, in treadmill running, under different conditions [NOR + placebo (PLA); NOR + PBMT; HYP + PLA; HYP + PBMT], in random order, to access energy contribution and maximal accumulated oxygen deficit alternative (MAODALT). Time to exhaustion and absolute aerobic contribution were lower in HYP than NOR, for both PLA and PBMT conditions (p < 0.05). In the percentage energy contribution of each metabolism during exhaustion effort, aerobic participation was lower, while MAODALT was higher in HYP than NOR, only in the PLA situation; alactic anaerobic participation was higher in HYP than NOR in the PBMT application situation (p = 0.002). The PBMT does not promote additional benefits over time to exhaustion in NOR and HYP conditions, however PBMT during exhaustive efforts in HYP results in an increase in anaerobiosis.
Objective: The aim of this study is to investigate the effect of modified holmium laser enucleation of the prostrate (HoLEP) on the function of urine control.
Methods: An analysis was conducted on a cohort of 305 elderly patients in a retrospective study spanning from February 2019 to February 2023. The cohort consisted of 150 patients who underwent modified HoLEP and 155 patients who underwent traditional HoLEP. Factors such as age, length of membranous urethra, prostate volume, BMI, IPSS, Qmax, enucleated weight, enucleation time, post-operative bladder irrigation time, catheterization and hospitalization were compared between both the groups. Multivariable logistic regression was used to identify independent predictors of urinary incontinence, and receiver operating characteristic (ROC) curve was used to determine the sensitivity and specificity of urethral membrane length in the diagnosis of urinary incontinence. Postoperative Qmax,IPSS, V2 were compared between 2 groups in the 6th month.
Results: Groups were compared in terms of age, volume, BMI, IPSS, Qmax and membranous urethral length (MUL). The time of enucleation, bladder irrigation, post-operative catheterization and hospitalization was lower in the modified HoLEP group (p < 0.05). The incidence of urinary incontinence (UI) in the modified group had a statistically significant decrease at 3 months (3.3% vs 9.7%, p = 0.025) and 6 months (0.6% vs 5.2%, p = 0.048). The median MUL had a significant difference in the 1st month (15.07 vs 12.5 mm, p < 0.001). In multivariate regression analysis, older age (OR per SD = 1.102, 95%CI: 1.077-1.136), shortened MUL (OR per SD = 0.776, 95%CI: 0.629-0.957) and increased enucleated prostate weight (OR per SD = 1.29,95%CI: 1.005-1.084) were significantly associated with UI. The ROC curve revealed the threshold value of MUL was 13.5 mm, the sensitivity was 76.7%, and the specificity was 98.1%.
Conclusion: The modified HoLEP technique is safe and effective on the function of urine control, and is superior to less residual prostate volume and higher maximum urine flow rate after surgery.
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