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Enterprise Credit Risk Decision: Application Based on Improved AHP 企业信贷风险决策:基于改进型 AHP 的应用
IF 0.6 Q2 Mathematics Pub Date : 2024-04-24 DOI: 10.11113/mjfas.v20n2.3311
Tao Li, Majid Khan, Majahar Ali, Tian Ying, Lili Wu
The credit risk of Shouguang vegetable enterprises in China is the biggest obstacle to corporate loans. Building a credit risk assessment model for Shouguang vegetable enterprises and accurately rating the risk of loan enterprises is the key to successful loans. This article aims to construct an AHP evaluation model for the credit risk of Shouguang vegetable enterprises. The data is sourced from managers, bank credit personnel, university researchers, and enterprise related customers who are familiar with the enterprise, while considering four risk levels: impact degree(I), occurrence probability(P), risk manageability(M), and government support(S). This article uses AHP and risk index scores to evaluate the credit risk of Shouguang vegetable enterprises. This model calculates the risk index score based on survey data from 41 corporate credit risk professionals, constructs a pairwise comparison judgment matrix, and conducts consistency testing. It calculates the risk level membership vectors of impact degree, occurrence probability, risk manageability, and government support level at four risk levels, and then calculates the comprehensive evaluation membership vector of credit risk for Shouguang vegetable enterprise. The calculation results show that the comprehensive credit risk assessment level of Shouguang vegetable enterprise belongs to the general risk level, with a membership value of 0.5836. The results still show that the credit rating of Shouguang vegetable enterprises in the four risk levels of impact degree, occurrence probability, risk manageability, and government support are all average risk levels, but there are differences in membership values. The maximum membership value under the impact degree level is 0.6163, and the minimum membership value under the risk manageability level is 0.5572. This study provides a feasible and practical model for enterprise credit risk assessment and conducts a detailed evaluation of the credit risk of Shouguang vegetable enterprise, providing valuable reference for enterprise managers, bank credit personnel, and related researchers.
我国寿光蔬菜企业的信用风险是企业贷款的最大障碍。建立寿光蔬菜企业信用风险评估模型,准确评定贷款企业的风险等级是贷款成功的关键。本文旨在构建寿光蔬菜企业信贷风险的 AHP 评估模型。数据来源于企业管理者、银行信贷人员、高校研究人员以及熟悉企业的相关客户,同时考虑了四个风险等级:影响程度(I)、发生概率(P)、风险可控性(M)和政府支持(S)。本文采用 AHP 法和风险指数评分法对寿光蔬菜企业的信用风险进行评价。该模型基于 41 位企业信用风险专业人士的调查数据计算风险指数分值,构建成对比较判断矩阵,并进行一致性检验。计算出四个风险等级的影响程度、发生概率、风险可控性、政府支持程度的风险等级成员向量,进而计算出寿光蔬菜企业信用风险综合评价成员向量。计算结果表明,寿光蔬菜企业的信用风险综合评价等级属于一般风险等级,成员值为 0.5836。结果仍显示,寿光蔬菜企业在影响程度、发生概率、风险可控性、政府支持四个风险等级中的信用等级均为一般风险等级,但成员值存在差异。影响程度水平下的最大成员值为 0.6163,风险可控水平下的最小成员值为 0.5572。本研究为企业信贷风险评估提供了一个切实可行的模型,对寿光蔬菜企业信贷风险进行了详细的评估,为企业管理者、银行信贷人员及相关研究人员提供了有价值的参考。
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引用次数: 0
Visualization of the Stingless Bee Research in Southeast Asia Region through Bibliometric Analysis using Scopus Database from 1984 to 2022 通过使用 Scopus 数据库对 1984 年至 2022 年东南亚地区的无刺蜜蜂研究进行文献计量分析,实现无刺蜜蜂研究的可视化
IF 0.6 Q2 Mathematics Pub Date : 2024-04-24 DOI: 10.11113/mjfas.v20n2.3058
Dg. Nooralizan Abd Wahid, Abu Bakar Abd Rahman, Ag Sufiyan Abd Hamid, M. Z. H. Makmud, Z. Jamain, Lucky Goh Poh Wah, Syed Umar Faruq Syed Najmuddin, Nur Zaida Zahari, Nurshafika Mohd Sakeh, M. K. Sabullah
The increasing activity of meliponiculture which plays a role as a natural agricultural pollinator as well as the production of stingless bee honey over time has encouraged high-impact research efforts around the world. Stingless bee honey is well known as a "superfood" product and has received significant attention due to its various applications such as the treatment of various diseases both traditionally and clinically. In this context, understanding interesting topics and expanding the network of collaborations is desirable to advance research towards integrated efforts, moreover, the Southeast Asia region (SEA) is one of the major revenue contributors to the global market, especially stingless bees. The purpose of this paper is to evaluate the research literature on stingless bee honey in the SEA region by conducting a bibliometric analysis of research papers in the Scopus database. A total of 543 journal articles published between 1984–2022 were obtained. The results have shown that since 2008, the average number of publications has increased by 35 articles per year, resulting in a steady increase in the number of publications accumulated until 2022. Only six countries are listed and almost 60% of total SEA publications are contributed by researchers from Malaysia, followed by Indonesia and Thailand which contribute 21% and 15%, respectively. In addition, among the top 10 most productive affiliates from every country in SEA, seven are universities in Malaysia, while Indonesia and Thailand have only one. The intellectual structure of research on stingless honey has been studied to offer a basic insight into the latest developments in this field of research, which will provide researchers and academics with broad guidelines for conducting further research in related fields.
无刺蜂是一种天然的农业授粉媒介,随着时间的推移,无刺蜂养殖和无刺蜂蜂蜜生产的日益活跃,促进了全世界范围内具有重大影响的研究工作。无刺蜂蜂蜜是众所周知的 "超级食品",因其在传统和临床上治疗各种疾病等各种应用而备受关注。此外,东南亚地区是全球市场的主要收入贡献者之一,尤其是无刺蜜蜂。本文旨在通过对 Scopus 数据库中的研究论文进行文献计量分析,评估东南亚地区无刺蜂蜂蜜的研究文献。共获得 1984-2022 年间发表的 543 篇期刊论文。结果表明,自 2008 年以来,平均每年增加 35 篇文章,使得 2022 年之前积累的论文数量稳步增长。只有 6 个国家榜上有名,在东南亚地区的出版物总数中,近 60% 由马来西亚的研究人员撰写,其次是印度尼西亚和泰国,分别占 21% 和 15%。此外,在东南亚地区每个国家排名前十的高产附属机构中,有七所是马来西亚的大学,而印度尼西亚和泰国只有一所。对无刺蜂蜜研究的知识结构进行研究,旨在对这一研究领域的最新进展有一个基本的了解,这将为研究人员和学者在相关领域开展进一步研究提供广泛的指导。
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引用次数: 0
Multimodal Convolutional Neural Networks for Sperm Motility and Concentration Predictions 用于精子活力和浓度预测的多模态卷积神经网络
IF 0.6 Q2 Mathematics Pub Date : 2024-04-24 DOI: 10.11113/mjfas.v20n2.3263
Voon Hueh Goh, Muhammad Asraf Mansor, M. A. As’ari, L. Ismail
Semen analysis is an important analysis for male infertility primary investigation and manual semen analysis is a conventional method to assess it. Manual semen analysis has been revealed with accuracy and precision limitations due to noncompliance to guidelines and procedures. Sperm motility and concentration are the main indicators for pregnancy and conception rate hence they were selected for parameters prediction. Convolutional neural network (CNN) has benefited computer vision application industry in recent years and has been widely applied in computer vision research tasks. In this paper, three-dimensional CNN (3DCNN) was designed to extract motion and temporal features, which are vital for sperm motility prediction. For sperm concentration, since two-dimensional CNN (2DCNN) is efficient in recognizing and extracting spatial features, well-established Residual Network (ResNet) architecture was adopted and customized for sperm concentration prediction. Multimodal learning approach is a technique to aggregate learnt features from different deep learning architecture that adopted other forms of modalities, which could provide deep learning model with better insights on their tasks. Hence, a multimodal learning deep learning architecture was designed to receive both image-based (frames extracted from video samples) and video-based (stacked frames pre-processed from video samples) input that could provide well-extracted spatial and temporal features for sperm parameters prediction.  The results obtained using the proposed methodology have surpassed other similar research works who used deep learning approach. For sperm motility, its best achieved average mean absolute error (MAE) was 8.048, and sperm concentration obtained a competent Pearson’s correlation coefficient (RP) value of 0.853.
精液分析是男性不育初诊的一项重要分析,而人工精液分析是评估男性不育的传统方法。人工精液分析由于不符合指导原则和程序,在准确性和精确性方面存在局限性。精子活力和浓度是怀孕率和受孕率的主要指标,因此被选为参数预测的指标。近年来,卷积神经网络(CNN)使计算机视觉应用行业受益匪浅,并被广泛应用于计算机视觉研究任务中。本文设计了三维卷积神经网络(3DCNN),以提取对精子活力预测至关重要的运动和时间特征。在精子浓度方面,由于二维 CNN(2DCNN)在识别和提取空间特征方面效率较高,因此采用了成熟的残差网络(ResNet)架构,并为精子浓度预测进行了定制。多模态学习方法是从不同的深度学习架构中汇总所学特征的技术,这些架构采用了其他形式的模态,可以为深度学习模型的任务提供更好的见解。因此,我们设计了一种多模态学习深度学习架构,以接收基于图像(从视频样本中提取的帧)和基于视频(从视频样本中预处理的堆叠帧)的输入,从而为精子参数预测提供提取良好的空间和时间特征。 使用所提方法获得的结果超过了使用深度学习方法的其他类似研究成果。在精子活力方面,其最佳平均绝对误差(MAE)为 8.048,精子浓度的皮尔逊相关系数(RP)为 0.853。
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引用次数: 0
Comparative Mitogenome Characterization of Cryptic Asian Seabass (Lates calcarifer) in Captivity within Peninsular Malaysia 马来西亚半岛人工饲养的隐性亚洲鲈鱼(Lates calcarifer)的有丝分裂基因组特征比较
IF 0.6 Q2 Mathematics Pub Date : 2024-04-24 DOI: 10.11113/mjfas.v20n2.3154
Athirah Mohd Bakri, Y. Esa
Lates calcarifer is one of the most commercial species in the world. Yet, phylogenetic relationships among this cryptic species remain unclear despite its significance in the fishery sector. The first report on bifurcation of captive Lates calcarifer in Peninsular Malaysia (PM) inferred from complete mitochondrial genome was discussed in the present study. Two mitochondrial genomes (mitogenomes) of Lates species were sequenced and assembled. The mitogenome length was 16,627 bp and 16,515 bp for K4 and S15, respectively. The majority of PCGs in both species exhibited the common initiation marker ATG codon and ended with termination marker TAA codon. Also, the incomplete termination codon T/TA was found in both species. Most AT-skew and GC-skew values observed in the protein-coding genes (PCGs) across the two samples were negative. The phylogenetic analysis based on 13 protein-coding genes by Maximum-Likelihood tree displayed two lineages from different regions which were from Myanmar/Indian waters (K4) and Southeast Asia/ Australian waters (S15). The striking genetic distance values between both specimens indicated 13 PCGs were suffering purifying selection. This study offers significant information for future analyses of evolutionary relationships among the numerous and taxonomically puzzling perciform fishes.
鲈鱼(Lates calcarifer)是世界上最具商业价值的鱼种之一。然而,尽管这一隐蔽物种在渔业领域具有重要意义,但其系统发育关系仍不清楚。本研究首次报道了根据完整的线粒体基因组推断出的马来西亚半岛(PM)人工饲养的金目鲈的分叉情况。研究人员对两种鲈鱼的线粒体基因组(有丝分裂基因组)进行了测序和组装。K4 和 S15 的线粒体基因组长度分别为 16,627 bp 和 16,515 bp。这两个物种的大多数 PCGs 都具有共同的起始标记 ATG 密码子,并以终止标记 TAA 密码子结束。此外,在这两个物种中还发现了不完全终止密码子 T/TA。在两个样本的蛋白质编码基因(PCGs)中观察到的 AT 偏移和 GC 偏移值大多为负值。基于 13 个蛋白编码基因的最大似然树系统进化分析表明,缅甸/印度海域(K4)和东南亚/澳大利亚海域(S15)有两个来自不同地区的品系。两个标本之间惊人的遗传距离值表明,13 个 PCGs 正在遭受净化选择。这项研究为今后分析种类繁多、分类学上令人困惑的鲈形目鱼类之间的进化关系提供了重要信息。
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引用次数: 0
Interval Neutrosophic Cubic Bézier Curve Approximation Model for Complex Data 复杂数据的区间中性立方贝塞尔曲线逼近模型
IF 0.6 Q2 Mathematics Pub Date : 2024-04-24 DOI: 10.11113/mjfas.v20n2.3240
Siti Nur Idara Rosli, M. I. E. Zulkifly
Complex data is defined as data that has the qualities of huge data, a lack of data information, and uncertainty. This paper discussed constructing the interval neutrosophic cubic Bézier curve (INCBC) approximation model for complex data. To construct the interval neutrosophic data point (INDP) based on the definition of interval neutrosophic set (INS), interval neutrosophic relation (INR) and interval neutrosophic point (INP). Next is the introduction of an interval neutrosophic control point (INCP) that blends with the theory of interval neutrosophic set and the Bernstein basis function. Later, the interval neutrosophic cubic Bézier curve (INCBC) model is visualizing with a four-by-four control points relation approximates the curves for truth, false, and indeterminacy membership. At the end of this paper will demonstrate the algorithm for the creation of the interval neutrosophic cubic Bézier curve (INCBC). The scientific value of this work is the acceptance of complex uncertainty data. As a result, due to the fact it combines fuzzy geometric modelling, this approach has the potential to make a significant contribution to complex uncertainty modelling by using this spline which is Bézier curve model.
复杂数据是指具有海量数据、数据信息匮乏和不确定性等特征的数据。本文讨论了构建复杂数据的区间中性立方贝塞尔曲线(INCBC)近似模型。根据区间中性集(INS)、区间中性关系(INR)和区间中性点(INP)的定义,构建区间中性数据点(INDP)。接着,介绍了与区间中性集理论和伯恩斯坦基函数相融合的区间中性控制点(INCP)。随后,利用四乘四控制点关系近似真、假和不确定成员关系曲线,将区间中性立方贝塞尔曲线(INCBC)模型可视化。本文最后将演示创建区间中性立方贝塞尔曲线(INCBC)的算法。这项工作的科学价值在于接受复杂的不确定性数据。因此,由于它结合了模糊几何建模,这种方法有可能通过使用这种贝塞尔曲线模型的样条线为复杂不确定性建模做出重大贡献。
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引用次数: 0
Investigation on Optimized Flavonoid Extraction from Leucas zeylanica and Its Anthelmintic Activity 关于从亮柏(Leucas zeylanica)中优化提取黄酮类化合物及其驱虫活性的研究
IF 0.6 Q2 Mathematics Pub Date : 2024-04-24 DOI: 10.11113/mjfas.v20n2.3464
Muhammad Luqman Selahuddeen, Marouane Bouguerra, Siti Ernieyanti Hashim, R. Abdul Wahab, A. F. A. Samad, Faizuan Abdullah
Extraction has been the primary method of concentrating and obtaining a crude essence of plants, and to an extend fruits and nuts. In modern times, there are many methods of extraction developed from simple maceration to using Soxhlet extractor to microwave assisted extraction and ultrasound assisted extraction. However, the more time saving and energy efficient method for the extraction of plants needs to be investigated. This research explored optimization of ultrasound assisted extraction of flavonoids from Leucas zeylanica and the optimization was done on parameters of sonication time (minutes), water bath temperature (°C), volume of solvent to solid ratio (ml/g) and solvent concentration (%) using response surface methodology (RSM). By measuring the total flavonoid content using aluminium colorimetric method and UV visible spectrophotometry, highest flavonoid yield is achieved with 30.38% increase compared to unoptimized method. This occurred with sonication time of 42 minutes, temperature of 55°C, solvent to solid ratio of 40 ml/g and solvent concentration of 100%. The resulting extract was then tested for its anthelmintic (anti-worm) ability, obtaining 40% efficacy of that of Albendazole.
萃取一直是浓缩和获取植物以及水果和坚果粗精华的主要方法。现代有许多萃取方法,从简单浸渍到使用索氏提取器,再到微波辅助萃取和超声波辅助萃取。然而,需要研究更省时省力的植物萃取方法。本研究探索了超声波辅助萃取白茅中黄酮类化合物的优化方法,并采用响应面方法(RSM)对超声时间(分钟)、水浴温度(°C)、溶剂体积与固体比率(毫升/克)和溶剂浓度(%)等参数进行了优化。通过使用铝比色法和紫外可见分光光度法测量总黄酮含量,与未优化的方法相比,黄酮产量最高,增加了 30.38%。超声时间为 42 分钟,温度为 55°C,溶剂与固体的比例为 40 毫升/克,溶剂浓度为 100%。然后对提取物进行了驱虫能力测试,结果显示其药效是阿苯达唑的 40%。
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引用次数: 0
Performance Measurement: Machine Learning as a Complement to DEA for Continuous Efficiency Estimation 绩效衡量:机器学习作为持续效率估算 DEA 的补充
IF 0.6 Q2 Mathematics Pub Date : 2024-04-24 DOI: 10.11113/mjfas.v20n2.3310
Yousef Khoubrane, Noor Asiah Ramli, Siti Shaliza Mohd Khairi
Data Envelopment Analysis (DEA) is a well-established non-parametric technique for performance measurement to access the efficiency of Decision-Making Units (DMUs). However, its inability to predict the efficiency values of new DMUs without re-conducting the analysis on the entire dataset has led to the integration of Machine Learning (ML) in previous studies to address this limitation. Yet, such integration often lacks a thorough evaluation of ML's adaptability in replacing current DEA process. This paper presents the results of an empirical study that employed eight ML models, two DEA variants, and a dataset of S&P500 companies. The findings demonstrated ML’s  remarkable precision in predicting efficiency values derived from a single DEA run and comparable performance in predicting the efficiency of new DMUs, thus eliminating the need for repeated DEA. This discovery highlights ML’s robustness as a complementary tool for DEA in continuous efficiency estimation, rendering the practice of re-conducting DEA unnecessary. Notably, boosting models within the Ensemble Learning category consistently outperformed other models, highlighting their effectiveness in the context of DEA efficiency prediction. Particularly, CatBoost demonstrated its superiority as the top-performing model, followed by LightGBM in the second position in most cases. When extended to five enlarged datasets, it shows that the model exhibits superior R² values in the CRS scenario.   
数据包络分析法(DEA)是一种成熟的非参数技术,用于衡量决策单位(DMU)的效率。然而,由于其无法在不对整个数据集重新进行分析的情况下预测新 DMU 的效率值,因此在以往的研究中引入了机器学习(ML)来解决这一局限性。然而,这种整合往往缺乏对 ML 在替代当前 DEA 过程中的适应性的全面评估。本文介绍了一项实证研究的结果,该研究采用了八个 ML 模型、两个 DEA 变体和一个 S&P500 公司数据集。研究结果表明,ML 在预测单次 DEA 运行得出的效率值方面具有极高的精确度,在预测新 DMU 的效率方面也有不相上下的表现,因此无需重复进行 DEA。这一发现凸显了 ML 在连续效率估计中作为 DEA 补充工具的稳健性,使重新进行 DEA 的做法变得没有必要。值得注意的是,"集合学习 "类别中的 "助推 "模型的表现始终优于其他模型,这凸显了它们在 DEA 效率预测方面的有效性。其中,CatBoost 模型表现尤为突出,成为表现最好的模型,LightGBM 在大多数情况下紧随其后,位居第二。当扩展到五个扩大的数据集时,结果表明该模型在 CRS 情景下表现出更优越的 R² 值。
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引用次数: 0
Generation of Multiwavelength Fibre Laser based on Erbium-Doped Fibre Amplifier with Lyot Filter 基于带 Lyot 滤波器的掺铒光纤放大器的多波长光纤激光器的产生
IF 0.6 Q2 Mathematics Pub Date : 2024-04-24 DOI: 10.11113/mjfas.v20n2.3091
S. Daud, Muhammad Ilham Ahmad Zaini, Muhammad Safwan Abd Aziz, A. F. Ahmad Noorden
A ring cavity setup consisting of erbium-doped fibre amplifier (EDFA) as the optical pump and lyot filter aids in the production of a multiwavelength spectrum. The wavelength of EDFA pumps in photons range of 1530 nm to 1560 nm, respectively. Using maximum power of 18 dBm and a single mode fibre (SMF) of 10 km length, the number of peak lasing lines produced between 3 dB spectral range is 51. Tuning of the polarization controller (PC) to a specific angle helps provide the most stable lasing line. Stability test is done on the output multiwavelength fibre laser (MWFL) over a time period to analyze the wavelength stability.
由掺铒光纤放大器(EDFA)作为光泵浦和莱奥特滤波器组成的环形腔装置有助于产生多波长光谱。EDFA 泵浦的光子波长范围分别为 1530 纳米到 1560 纳米。使用最大功率为 18 dBm、长度为 10 千米的单模光纤(SMF),在 3 dB 光谱范围内产生的峰值激光线数量为 51 条。将偏振控制器(PC)调至特定角度有助于提供最稳定的激光线。对输出的多波长光纤激光器(MWFL)进行了一段时间的稳定性测试,以分析波长的稳定性。
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引用次数: 0
Characteristic Polynomial of Power Graph for Dihedral Groups Using Degree-Based Matrices 使用基于度数的矩阵计算二面形群幂图的特征多项式
IF 0.6 Q2 Mathematics Pub Date : 2024-04-24 DOI: 10.11113/mjfas.v20n2.3357
M. U. Romdhini, A. Nawawi, F. Al-Sharqi, A. Al-Quran
A fundamental feature of spectral graph theory is the correspondence between matrix and graph. As a result of this relation, the characteristic polynomial of the graph can be formulated. This research focuses on the power graph of dihedral groups using degree-based matrices. Throughout this paper, we formulate the characteristic polynomial of the power graph of dihedral groups based on seven types of graph matrices which include the maximum degree, the minimum degree, the greatest common divisor degree, the first Zagreb, the second Zagreb, the misbalance degree, and the Nirmala matrices.
谱图理论的一个基本特征是矩阵与图之间的对应关系。由于这种关系,可以提出图的特征多项式。本文的研究重点是使用基于度的矩阵研究二面群的幂图。在本文中,我们根据七种图矩阵(包括最大度矩阵、最小度矩阵、最大公因子矩阵、第一萨格勒布矩阵、第二萨格勒布矩阵、不平衡度矩阵和尼玛拉矩阵)来计算二面群幂图的特征多项式。
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引用次数: 0
Identification of MiR398 and Its Regulatory Roles in Terpenoid Biosynthesis of Persicaria odorata 鉴定 MiR398 及其在柿树萜类生物合成中的调控作用
IF 0.6 Q2 Mathematics Pub Date : 2024-04-24 DOI: 10.11113/mjfas.v20n2.3248
Nursyah Fitri Mahadi, Azman Abd Samad, A. F. A. Samad
Persicaria odorata is an herbaceous plant with antifungal and antibacterial properties. The plant produces secondary metabolites, including phenols, sulphur-containing compounds and terpenoids, in response to biotic and abiotic stresses. Terpenoids in P. odorata are synthesized through the mevalonate (MVA) and the methylerythritol phosphate (MEP) pathways. 1-deoxy-D-xylulose-5phosphate isomerase (DXR) is a rate-limiting enzyme in the MEP pathway and may be regulated by microRNA (miRNA) miR398. There is a lack of evidence showing miR398 regulation in terpenoid biosynthesis in P. odorata through DXR-targeting. The study aimed to verify the stem-loop structure of miR398 and analyse its expression towards the target gene, DXR, in treated and control P. odorata. RNA was quantified and qualitatively analysed using a Nanodrop spectrophotometer and agarose gel electrophoresis. The stem loop of miR398 was verified using reverse transcriptase PCR (RT-PCR) and agarose gel electrophoresis. The expression of miR398 and DXR was compared between control and treated samples. Treated leaves were punctured with needles and left for 48h before harvest. Gene expressions were quantified and normalised using reference genes. The stem-loop structure of miR398 was confirmed, despite possible primer mismatches and unspecific binding. This step was essential before comparing and assessing the gene expression of miR398 and DXR. A decrease in abundance of miR398 whereas in increase in abundance was in the treated sample compared to the control sample indicating that miR398 negatively regulated DXR under stress conditions, suggesting an increase in terpenoid synthesis as a defence mechanism. DXR acts as a rate-limiting enzyme in the MEP metabolic pathway. Further studies are needed to quantify the effects of miR398 on terpenoid biosynthesis after wounding in P. odorata.
Persicaria odorata 是一种草本植物,具有抗真菌和抗菌特性。该植物会产生次级代谢产物,包括酚类、含硫化合物和萜类化合物,以应对生物和非生物压力。臭腥草中的萜类化合物是通过甲羟戊酸(MVA)和季戊四醇磷酸酯(MEP)途径合成的。1-deoxy-D-xylulose-5phosphate isomerase (DXR) 是 MEP 途径中的限速酶,可能受 microRNA(miRNA)miR398 的调控。目前还没有证据表明 miR398 通过 DXR 靶向调控臭腥草中萜类化合物的生物合成。本研究旨在验证 miR398 的茎环结构,并分析其在处理的臭腥草和对照的臭腥草中对目标基因 DXR 的表达。使用 Nanodrop 分光光度计和琼脂糖凝胶电泳对 RNA 进行定量和定性分析。使用反转录酶 PCR(RT-PCR)和琼脂糖凝胶电泳验证了 miR398 的茎环。比较对照样本和处理样本中 miR398 和 DXR 的表达情况。用针刺入处理过的叶片,放置 48 小时后采收。使用参考基因对基因表达进行量化和归一化。尽管可能存在引物错配和非特异性结合,miR398 的茎环结构还是得到了证实。在比较和评估 miR398 和 DXR 的基因表达之前,这一步骤至关重要。与对照样本相比,处理样本中 miR398 的丰度下降,而 DXR 的丰度上升,这表明 miR398 在胁迫条件下对 DXR 起负向调节作用,这表明萜类化合物合成增加是一种防御机制。DXR 是 MEP 代谢途径中的限速酶。要量化 miR398 对臭椿受伤后萜类化合物生物合成的影响,还需要进一步的研究。
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引用次数: 0
期刊
Malaysian Journal of Fundamental and Applied Sciences
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