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Sequencing of 18S rRNA gene of Bdelloid rotifers and design of the primers for real-time PCR 轮虫18S rRNA基因测序及实时PCR引物设计
Pub Date : 2019-12-31 DOI: 10.54279/mijeec.v1i3.244932
Watcharapong Thakong, Kazuya Shimizu, Miwa Kodato, Norio Iwami, Niwooti Whangchai, Tomoaki Itayama
Three individuals of Bdelloid rotifer (J1, J2 and J3) were isolated from a MBR system in Nagasaki University and one individual of rotifer (J4) in the original seed sludge collected from a wastewater treatment plant for the MBR was isolated. The four rotifer species were able to proliferate in toxic Microcystis cell suspension. The partial sequence of 18S rRNA gene of each isolated rotifer was determined using In-fusion cloning and searched by BLAST. The gene of the four rotifers J1, J2, J3 and J4 showed the same sequence, then the consensus sequence was in the branch of Bdelloid rotifers in the phylogenetic tree. Furthermore, a specific Bdelloid forward primer 55F and reverse primer 395R for real-time PCR was designed based on the consensus sequence for quantitative researches on the Bdelloid rotifers population. We succeeded to quantify the population of a Bdelloid rotifer cultured in toxic Microcystis cell suspension using the new designed primer pairs.
从长崎大学的MBR系统中分离出3只蛭形轮虫(J1、J2和J3),从某污水处理厂的MBR原始种子污泥中分离出1只轮虫(J4)。四种轮虫均能在毒微囊藻细胞悬浮液中增殖。采用In-fusion克隆法确定各分离轮虫18S rRNA基因的部分序列,并用BLAST进行检索。4种轮虫J1、J2、J3和J4的基因序列一致,在系统发育树中一致的序列位于蛭形轮虫分支。根据蛭形轮虫群体定量研究的共识序列,设计了蛭形轮虫实时PCR特异性正向引物55F和反向引物395R。我们成功地用新设计的引物对在有毒微囊藻细胞悬液中培养的蛭形轮虫种群进行了量化。
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引用次数: 0
Analysis on a vibration character of hollow fiber membrane bundle in MBR MBR中空纤维膜束振动特性分析
Pub Date : 2019-12-25 DOI: 10.54279/mijeec.v1i3.244930
Prattakorn Sittisom, Yoonjae I, Tomoaki Itayama
We have focused on membrane vibration in MBR to find an effective design for the reduction of membrane fouling. In the previous study, we developed a direct measurement method for membrane vibration of a hollow fiber membrane (HFM) using an accelerometer (ACM). In this study, we studied on vibration characters on an HFM bundle in a practical membrane module in MBR using the ACMs in a large transparent water tank. Three ACMs were attached at the middle (P1), top (P2) and bottom (P3) position along a center line in the HFM bundle in which air was supplied from a diffuser below the membrane module with different aeration rates from 0 to 250 L/min. The acceleration of membrane vibration time series for the X-axis direction (left-right displacement) and Z-axis direction (back-front displacement) was recorded at three positions. The average vibration amplitudes of the acceleration along both directions at each position were increased as the aeration rate was increased. The HFM bundle showed a collective vibration with a frequency peak between 0 and 50 Hz. The Z-axis motion character of HFM bundle is regarded as a sheet vibration. The obtained vibration character was useful for the new design of a membrane module in MBR against the membrane fouling.
为了找到一种有效的减少膜污染的设计方法,我们对MBR中的膜振动进行了研究。在之前的研究中,我们开发了一种使用加速度计(ACM)直接测量中空纤维膜(HFM)膜振动的方法。在本研究中,我们使用大型透明水箱中的ACMs,研究了MBR中实际膜模块中HFM束的振动特性。在HFM束的中间(P1)、顶部(P2)和底部(P3)位置沿中线连接3个acm,其中空气由膜组件下方的扩散器提供,曝气率从0到250 L/min不等。在三个位置分别记录x轴方向(左右位移)和z轴方向(前后位移)的膜振动时间序列加速度。各位置沿两个方向加速度的平均振动幅值随曝气率的增加而增大。HFM束呈现集体振动,频率峰值在0 ~ 50hz之间。将高频调频束的z轴运动特征视为片状振动。所获得的振动特性对MBR中膜组件抗膜污染的新设计具有指导意义。
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引用次数: 0
Green biomass to biogas – A study on anaerobic monodigestion of para grass 绿色生物质转化为沼气——草料厌氧单消化的研究
Pub Date : 2019-12-05 DOI: 10.54279/mijeec.v1i3.244927
Ajcharapa Chuanchai, Sawitree Tipnee, Yuwalee Unpaprom, Keng-Tung Wu
Recently, biogas production through anaerobic digestion technology has advanced massively. At the moment, caused by high energy demand and environmental concerns as the world’s population increases, the drive for anaerobic digestion processes is achievement drive within research and the industry for sustainable energy generation. The study evaluated biogas production from anaerobic mono-digestion of para grass in laboratory scale studies. In addition, improvement of the biogas yield from the grass via chemical pretreatment and leaching bed reactors was studied. Methane content of biogas was 54.36 % by mono- substrate. The results revealed that para grass can be treated anaerobically and are a good source of biogas.
近年来,利用厌氧消化技术生产沼气取得了长足的进步。目前,随着世界人口的增加,由于能源需求和环境问题的增加,厌氧消化过程的驱动力是研究和可持续能源生产行业的成就驱动力。该研究在实验室规模研究中评估了对生草厌氧单消化的沼气产量。此外,还研究了化学预处理和浸出床反应器对草料沼气产率的提高。单一基质下沼气甲烷含量为54.36%。结果表明,草料可厌氧处理,是一种良好的沼气来源。
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引用次数: 0
Smart and green university campus 智能绿色大学校园
Pub Date : 2019-11-30 DOI: 10.54279/mijeec.v1i3.244926
Nobutaka Ito
Many devices connected to the internet are able to generate valuable data that can help create technologically connected smart and green university campuses. These campuses can electronically monitor activities, including student attendance, security operations, campus lighting, and classroom usage that administrators, staff, and faculty members can use to make better decisions about how to operate more efficiently, effectively, and safely. University campuses have a responsibility not only to provide a quality education for students but also to provide a safe place to learn and operate with an appropriate allocation of resources applying with smart system with reasonable approaches.
许多连接到互联网的设备能够产生有价值的数据,这些数据可以帮助创建技术连接的智能和绿色大学校园。这些校园可以电子监控活动,包括学生出勤、安全操作、校园照明和教室使用情况,管理员、员工和教职员工可以使用这些活动来更好地决定如何更高效、更有效和更安全地运营。大学校园不仅有责任为学生提供优质的教育,而且有责任为学生提供一个安全的学习和操作场所,合理分配资源,应用智能系统和合理的方法。
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引用次数: 0
Desalination of Polymer and Chemical industrial wastewater by using green photosynthetic microalgae, Chlorella sp. 绿色光合微藻(Chlorella sp.)在聚合物及化工废水淡化中的应用。
Pub Date : 2019-10-30 DOI: 10.54279/mijeec.v1i3.244924
Prakash Bhuyar, Dang Diem Hong, Emelina Mandia, Mohd Hasbi Ab. Rahim, Gaanty Pragas Maniam, Natanamurugaraj Govindan
In this investigation microalgae Chlorella sp. were isolated and identified from the industrial wastewater. Microalgae species was mass cultivated by using BG11 medium. After 30 days, mixture of Chlorella with different wastewater A, B, C, D, E and F with different ration of Chlorella: wastewater which were 1:6, 1:1, and 2:1. Incubated at room temperature at illuminated area. Dissolved oxygen, TDS, salinity, pH, optical density, oxygen saturation and conductivity were measured for day 0, 10, 20 and 30. For microalgae in wastewater A and C, value of pH, salinity, dissolved oxygen, oxygen saturation, conductivity, salinity and TDS did not change while absorbance value decreased from day 0 to day 30. For wastewater B, D, E and F, the absorbance and pH value increase for all concentration from day 0 to day 30. The highest oxygen saturation after 30 days for wastewater B, D, E and F was at concentration 1:6, 2:1 and 1:1 respectively. The highest dissolved oxygen for wastewater B, D and E was at concentration 1:1 and F was at 2:1. The lowest conductivity, salinity and TDS for wastewater B, C, D and F were all at the concentration of 2:1. The result showed that, Chlorella managed to reduce the salinity for wastewater B, C, D and F, at concentration of 2:1 which were 3.67 %, 4.53 %, 5.4 % and 4.91 % respectively.
本研究从工业废水中分离鉴定了小球藻。采用BG11培养基对微藻进行了批量培养。30天后,将小球藻与不同废水A、B、C、D、E、F混合,小球藻与废水的比例分别为1:6、1:1、2:1。在室温下光照区域孵育。在第0、10、20和30天测定溶解氧、TDS、盐度、pH、光密度、氧饱和度和电导率。从第0天到第30天,A和C废水中微藻的pH、盐度、溶解氧、氧饱和度、电导率、盐度和TDS值没有变化,而吸光度值有所下降。对于废水B、D、E、F,从第0天到第30天,所有浓度的吸光度和pH值都增加。废水B、D、E和F浓度分别为1:6、2:1和1:1时,30 D后氧饱和度最高。废水B、D、E溶解氧浓度为1:1,F浓度为2:1时溶解氧浓度最高。废水B、C、D、F的电导率、盐度和TDS均为2:1的浓度时最低。结果表明,在2:1的浓度下,小球藻对废水B、C、D和F的盐度分别为3.67%、4.53%、5.4%和4.91%。
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引用次数: 7
Evaluation of mango, longan and lychee trees pruning leaves for the production of biogas via anaerobic fermentation 芒果、龙眼和荔枝树修剪叶片厌氧发酵产沼气的研究
Pub Date : 2019-10-15 DOI: 10.54279/mijeec.v1i3.244925
Yuwalee Unpaprom, Nuttapong Saetang, Sawitree Tipnee
Pruning fruit trees is improving their full health and harvest. Fruit tree pruning leaves waste to represent an abundant amount of organic materials, and these produced during a short period. The basic fuel properties of lignocellulosic biomass from orchards were evaluated on the following fruit tree leaves obtained from pruning operations. Biomass has become a vital source of renewable energy. Biogas is one the renewable energy which can be produced by anaerobic fermentation of biomass. In this study, mango, longan and lychee trees pruning leaves waste was utilized for biogas production. These leaves were examined on proximate analysis and ultimate analysis contents are considered as carbon (C), hydrogen (H), oxygen (O), nitrogen (N) and sulfur (S). In this study, pretreatment was performed using a sodium hydroxide solution (w/v) at different concentrations as above (1, 2, 3, and 4%). For the best feedstock screening, the theoretical biochemical methane potential was confirmed. Mango leaves biomethane content was higher compared to longan and lychee leaves. Finally, this biomass was suggested further large-scale studies. Digestate from biogas system is a highly valuable nutrient and rich fertilizer.
修剪果树可以提高果树的健康和收成。果树修剪后的叶片废弃物代表了大量的有机物质,而且这些有机物质是在短时间内产生的。以果树修剪后的叶片为研究对象,对果园木质纤维素生物质的基本燃料特性进行了评价。生物质能已成为可再生能源的重要来源。沼气是一种由生物质厌氧发酵产生的可再生能源。本研究利用芒果、龙眼和荔枝树修剪叶片废弃物生产沼气。这些叶片进行了近似分析,最终分析的含量被认为是碳(C)、氢(H)、氧(O)、氮(N)和硫(S)。在本研究中,使用不同浓度(1、2、3和4%)的氢氧化钠溶液(w/v)进行预处理。为筛选最佳原料,确定了理论生化甲烷势。芒果叶生物甲烷含量高于龙眼叶和荔枝叶。最后,提出了进一步大规模研究的建议。沼气消化液是一种高价值的营养物和富营养肥。
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引用次数: 0
Development of a novel sequential pretreatment strategy for the production of bioethanol from coconut pulp residue 椰浆渣生产生物乙醇的新型顺序预处理策略的开发
Pub Date : 2019-09-30 DOI: 10.54279/mijeec.v1i3.244920
Alissandra Pauline B. Mariano, Y. Unpaprom, R. Ramaraj
Coconut pulp residues waste generated after extraction of milk or oil. These wastes end up as feed to animals, fertilizers and firewood/cooking fuel whilst large quantities often left to rot in the field, which causes cause pollution, waste disposal problems and increase handling cost for farmers. In order to alleviate this problem, coconut pulp residue was used as feedstock for bioethanol production. However, improvements on pretreatment are necessary to produce higher sugar concentration prior to fermentation. Bioethanol production from coconut pure pulp residue (PPR) and combined pulp residue (CPRS) was investigated. The results showing 40 minutes’ pre-hydrothermal treatment time and 2% mild sulphuric acid for PPR and 20 minutes’ hydrothermal treatment time and 2% mild sulphuric acid for CPRS.
椰浆残渣提取奶或油后产生的废弃物。这些废物最终被用作动物饲料、肥料和柴火/烹饪燃料,而大量的废物往往被留在田间腐烂,这造成了污染、废物处理问题,并增加了农民的处理成本。为了缓解这一问题,以椰子浆渣为原料生产生物乙醇。然而,为了在发酵前产生更高的糖浓度,需要改进预处理。研究了以椰子纯浆渣(PPR)和复合浆渣(CPRS)为原料生产生物乙醇。结果表明:预水热处理时间为40 min,淡硫酸用量为2%;预水热处理时间为20 min,淡硫酸用量为2%。
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引用次数: 1
University goods manufacturing program combined with engineering education 大学商品制造专业与工程教育相结合
Pub Date : 2019-08-30 DOI: 10.54279/mijeec.v1i2.244919
Nobutaka Ito
Due to the frequent university reforms, the level of understanding higher education and conducting research programs are getting lower in recent ten years. Their knowledge level sometimes discourages and disappoints the university professor. Their active, challenging attitude to know and integrate the knowledge based on the experiences cannot be seen in most of the university students. They need to know anything about practical application. This paper is one of the proposals to provide the opportunity for university students to learn and experience more practicing through university goods manufacturing based on the CNC machine operated on computer programming.
近十年来,由于高校改革的频繁,人们对高等教育的认识和开展研究项目的水平越来越低。他们的知识水平有时会使大学教授气馁和失望。他们积极、挑战的态度,在经验的基础上认识和整合知识,这在大多数大学生中是看不到的。他们需要了解实际应用。本文是通过计算机编程操作的数控机床制造大学用品,为大学生提供学习和实践的机会之一。
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引用次数: 0
Influential degree of polymerization of sugar extraction on alkali pretreatment from sunflower stalk wastes by applied statistical modelling 应用统计模型研究了糖提取聚合度对向日葵秸秆废弃物碱预处理的影响
Pub Date : 2019-08-20 DOI: 10.54279/mijeec.v1i2.244917
Numchok Manmai, Nuttapong Saetang, Yuwalee Unpaprom, Keng-Tung Wu
This paper presents application of influence of degree of polymerization (DP) on optimally pretreated using a response surface methodology (RSM) approach for decreased DP level of optimal chemical and biological pretreatments from sunflower stalk. All experiments in this paper are applied by statistical designs for developing a statistic multifunction analysis model that focus on the effect of dissimilar factors for describing of the optimum values of the changed surface response on any variables. The process parameters of chemical model (Sodium Hydroxide concentration and Time) to pretreat for DP. The chemical pretreatment model was certified by 13 runs, at two factors, NaOH (1, 1.5, 2%) and Day (1, 2, 3) by central composite design (CCD). DP value of the chemical model was estimated by a Design Expert program version 11 trial: chemical model of DP highest and lowest of 25.80 and 6.16, consecutively. The aim of this experiments to investigate only DP from the chemical model of pretreatment. The procedure there are effective on sugar conversion and DP of the lignocellulosic biomass. Which pretreatment is a challenge for cost and competitive technology on large-scale of fermentable sugar in the step of hydrolysis.
本文应用响应面法研究了聚合度对最佳预处理的影响,降低了向日葵秸秆的最佳化学和生物预处理的聚合度。本文中所有的实验都应用于统计设计,以建立一个统计多功能分析模型,该模型关注不同因素的影响,以描述变化的表面响应对任何变量的最佳值。化学模型的工艺参数(氢氧化钠浓度和时间)预处理的DP。采用中心复合设计(CCD),在NaOH(1,1.5, 2%)和Day(1,2,3)两个因素下,对化学预处理模型进行了13次运行验证。化学模型的DP值通过Design Expert程序version 11试验估计:化学模型的DP最高和最低值依次为25.80和6.16。本实验目的仅从预处理的化学模型探讨DP。该工艺对木质纤维素生物质的糖转化和DP均有较好的效果。可发酵糖在水解过程中的大规模预处理是成本和竞争技术的挑战。
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引用次数: 1
Study on performance of a savonius wind turbines related with the blade angle 萨伏纽斯型风力机性能与叶片角度的关系研究
Pub Date : 2019-08-09 DOI: 10.54279/mijeec.v1i2.244916
Saowalak Thongdee, Churat Tararuk, Natthawud Dussadee, Rameshprabu Ramaraj, Tanate Chaichana
This research aimed to compare the performance of Savonius vertical axis wind turbines through blade numbers and different blade angles. In this study, applicable turbines having 4, 6, 8, 12, 16 and 18 numbers of blades with the angles of the blades of -15°, -5°, 0°, 5° and 15°, respectively. The rotor used was a semicircle shaped blade made from PVC material and has a blade diameter of 6 cm and 30 cm for both rotor diameter and height. The turbine was tested deadweight range of 0-0.49 kg at 4 m/s wind speed. The results showed that the blade angle has a positive effect on increasing the power and torque coefficient of Savonius wind turbine, specifically on blades less than 16. The highest power and torque coefficient was obtained from the turbine having16 blades at an angle of 5°. This configuration also found that the maximum power and torque coefficient in the tip speed ratio ranging from 0.3-0.4 are 0.2519 and 0.5858, respectively.
本研究旨在通过叶片数和不同叶片角度来比较Savonius垂直轴风力机的性能。在本研究中,适用的涡轮叶片数分别为4、6、8、12、16和18,叶片角度分别为-15°、-5°、0°、5°和15°。使用的转子是由PVC材料制成的半圆形叶片,转子直径和高度的叶片直径分别为6厘米和30厘米。风力机在4米/秒风速下的自重范围为0-0.49 kg。结果表明,叶片角度对Savonius风力机功率和转矩系数的提高有积极作用,特别是对小于16的叶片。16片叶片成5°角的涡轮功率和扭矩系数最高。该配置还发现,在0.3 ~ 0.4的叶尖速比范围内,最大功率和扭矩系数分别为0.2519和0.5858。
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引用次数: 0
期刊
Maejo International Journal of Energy and Environmental Communication
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