Zhe Ye, Lingling Long, Sizhu Long, Min Yang, Ming Tan
Surgical resection is the main treatment approach for gastric cancer (GC), but surgical anesthetics may have an impact on postoperative cognitive function. Therefore, this study investigated the effect of liensinine on neuronal apoptosis and cognitive function in mice with GC induced by dexmedetomidine anesthesia. Firstly, a GC mouse model was established and divided into the following groups; blank control group, GC model group, low-dose (4 μ mol/L) and high-dose (8 μ mol/L) groups of liensinine ( n = 6), to detect apoptosis of neurons. In addition, the GC model group, Synuclein (SYN) mimic group, SYN inhibitor group, high-dose neusinine group, and high-dose liensinine+SYN inhibitor group were set up with 6 rats in each group. The cognitive function of mice after treatment was observed by Morris water maze experiment, and neuronal cell apoptosis and expressions of SYN, brain-derived neurotrophic facto (BDNF) and Caspase-3 were explored. Liensinine significantly improved the cognitive function of GC mice after dexmedetomidine anesthesia, and this process is related to decreased SYN expression. Liensinine can inhibit increased SYN expression, so apoptosis of neurons in the hippocampus of mice was controlled after the use of SYN inhibitors, especially in the high-dose liensinine+SYN inhibitor group. Liensinine down-regulated the expression of SYN and inhibited the Caspase-3 to reduce neuronal cell apoptosis, promoting recovery of BDNF level, and then playing role in improving the cognitive function of GC mice after dexmedetomidine anesthesia.
{"title":"Liensinine improves cognitive function of gastric cancer under dexmedetomidine anesthesia by inhibiting neuron apoptosis through synuclein","authors":"Zhe Ye, Lingling Long, Sizhu Long, Min Yang, Ming Tan","doi":"10.1166/mex.2023.2502","DOIUrl":"https://doi.org/10.1166/mex.2023.2502","url":null,"abstract":"Surgical resection is the main treatment approach for gastric cancer (GC), but surgical anesthetics may have an impact on postoperative cognitive function. Therefore, this study investigated the effect of liensinine on neuronal apoptosis and cognitive function in mice with GC induced by dexmedetomidine anesthesia. Firstly, a GC mouse model was established and divided into the following groups; blank control group, GC model group, low-dose (4 μ mol/L) and high-dose (8 μ mol/L) groups of liensinine ( n = 6), to detect apoptosis of neurons. In addition, the GC model group, Synuclein (SYN) mimic group, SYN inhibitor group, high-dose neusinine group, and high-dose liensinine+SYN inhibitor group were set up with 6 rats in each group. The cognitive function of mice after treatment was observed by Morris water maze experiment, and neuronal cell apoptosis and expressions of SYN, brain-derived neurotrophic facto (BDNF) and Caspase-3 were explored. Liensinine significantly improved the cognitive function of GC mice after dexmedetomidine anesthesia, and this process is related to decreased SYN expression. Liensinine can inhibit increased SYN expression, so apoptosis of neurons in the hippocampus of mice was controlled after the use of SYN inhibitors, especially in the high-dose liensinine+SYN inhibitor group. Liensinine down-regulated the expression of SYN and inhibited the Caspase-3 to reduce neuronal cell apoptosis, promoting recovery of BDNF level, and then playing role in improving the cognitive function of GC mice after dexmedetomidine anesthesia.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135349463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yingjun Wu, Xiaoyuan Bu, Xinyu Zhou, Zhilin Sha, Xintong Shi
This study investigates the efficacy of N-Alkyl-polyethylenimine 2 kDa–stabilized superparamagnetic iron oxide ((PEI2k/SPIO) nanoparticles on hepatocellular carcinoma (HCC) in mice and explored the underlying mechanism. Highly metastatic HCC cells were cultured and mRNA expressions of c-MET and Ets-1 were determined by Reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Cell viability was detected by CCK-8 and apoptosis was assessed by flow cytometry. After establishment of animal model for HCC, the rats were administered PEI2k/SPIO nanoparticles and/or Ets-1 inhibitor through tail vein. Cell apoptosis and proliferation were then assessed by EdU experiment and flow cytometry, and the levels of c-MET, Ets-1, MMP-2 were measured as well. HCC cells presented up-regulated c-MET and down-regulated Ets-1. Treatment with PEI2k/SPIO nanoparticles resulted in decreased in c-MET expression and increased Ets-1 in both cells and animals. The PEI2k/SPIO nanoparticles significantly decreased cell proliferation and suppressed tumor growth, and induced apoptosis. Besides, additional injection of Ets-1 enhanced phosphorylation activity of MMP-2 and alleviated PEI2k/SPIO’s effect on MMP-2 expression. Nanotechnology is known to improve delivery efficiency and hence affect prognosis. This study elucidated that, PEI2k/SPIO nanoparticles suppressed malignant characteristics of HCC cells and tumor growth through down-regulation of c-MET and growth factors and up-regulation of MMP-2 and Ets-1.
{"title":"Polyethylenimine 2k (PEI2k)/superparamagnetic iron oxide (SPIO) nanoparticle inhibits development of hepatocellular carcinoma through targeting of c-MET and Ets-1","authors":"Yingjun Wu, Xiaoyuan Bu, Xinyu Zhou, Zhilin Sha, Xintong Shi","doi":"10.1166/mex.2023.2490","DOIUrl":"https://doi.org/10.1166/mex.2023.2490","url":null,"abstract":"This study investigates the efficacy of N-Alkyl-polyethylenimine 2 kDa–stabilized superparamagnetic iron oxide ((PEI2k/SPIO) nanoparticles on hepatocellular carcinoma (HCC) in mice and explored the underlying mechanism. Highly metastatic HCC cells were cultured and mRNA expressions of c-MET and Ets-1 were determined by Reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Cell viability was detected by CCK-8 and apoptosis was assessed by flow cytometry. After establishment of animal model for HCC, the rats were administered PEI2k/SPIO nanoparticles and/or Ets-1 inhibitor through tail vein. Cell apoptosis and proliferation were then assessed by EdU experiment and flow cytometry, and the levels of c-MET, Ets-1, MMP-2 were measured as well. HCC cells presented up-regulated c-MET and down-regulated Ets-1. Treatment with PEI2k/SPIO nanoparticles resulted in decreased in c-MET expression and increased Ets-1 in both cells and animals. The PEI2k/SPIO nanoparticles significantly decreased cell proliferation and suppressed tumor growth, and induced apoptosis. Besides, additional injection of Ets-1 enhanced phosphorylation activity of MMP-2 and alleviated PEI2k/SPIO’s effect on MMP-2 expression. Nanotechnology is known to improve delivery efficiency and hence affect prognosis. This study elucidated that, PEI2k/SPIO nanoparticles suppressed malignant characteristics of HCC cells and tumor growth through down-regulation of c-MET and growth factors and up-regulation of MMP-2 and Ets-1.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135349283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To explore the correlation of tumor necrosis factor receptor-associated protein (TRAP1) and myeloid-derived suppressor cells (MDSCs) in peripheral blood with clinical treatment efficacy and prognosis in nonsmall-cell lung cancer (NSCLC). The study cohort comprised 112 patients with NSCLC who were treated between March 2020 to December 2022, including 45 and 67 patients with stage I–II and III–VI NSCLC, respectively, and 90 healthy individuals as controls. Peripheral blood samples of study participants before and after chemotherapy were collected. Serum MDSC and TRAP1 levels were compared in patients categorized according to the NSCLC stage by nanomagnetic bead-based separation method. Flow cytometry was used to further analyze the expression levels of MDSCs and TRAP1 in cells before and after chemotherapy in patients with NSCLC. Chemotherapy efficacy was evaluated according to the World Health Organization objective evaluation criteria for tumor efficacy, and the relationship of chemotherapy efficacy with serum MDSC and TRAP1 levels was analyzed. Multivariate Cox regression analysis was conducted to evaluate factors associated with prognosis. The serum levels of TRAP1 and MDSCs were significantly higher in patients with NSCLC than in healthy controls ( P <0.05). Serum TRAP1 and MDSC levels were positively correlated with TNM stage. Serum levels of TRAP1 and MDSCs after chemotherapy were significantly lower than those before chemotherapy in the overall cohort, serum levels of TRAP1 after chemotherapy were significantly lower than those before chemotherapy in patients with partial response, and serum levels of MDSCs after chemotherapy were significantly higher than those before chemotherapy in patients with progressive disease ( P < 0.05). Multivariate Cox regression analysis revealed that high serum levels of TRAP1 and MDSCs were associated with poor prognosis. Serum levels of TRAP1 and MDSCs should be considered as potential predictive biomarkers for chemotherapy efficacy and prognosis in NSCLC.
{"title":"Nanomagnetic bead-based nucleic acid isolation to examine the correlation of serum TRAP1 and MDSC levels with clinical treatment efficacy and prognosis in nonsmall cell lung cancer","authors":"Shan Dai, Nan Dai, Jie Zhang","doi":"10.1166/mex.2023.2501","DOIUrl":"https://doi.org/10.1166/mex.2023.2501","url":null,"abstract":"To explore the correlation of tumor necrosis factor receptor-associated protein (TRAP1) and myeloid-derived suppressor cells (MDSCs) in peripheral blood with clinical treatment efficacy and prognosis in nonsmall-cell lung cancer (NSCLC). The study cohort comprised 112 patients with NSCLC who were treated between March 2020 to December 2022, including 45 and 67 patients with stage I–II and III–VI NSCLC, respectively, and 90 healthy individuals as controls. Peripheral blood samples of study participants before and after chemotherapy were collected. Serum MDSC and TRAP1 levels were compared in patients categorized according to the NSCLC stage by nanomagnetic bead-based separation method. Flow cytometry was used to further analyze the expression levels of MDSCs and TRAP1 in cells before and after chemotherapy in patients with NSCLC. Chemotherapy efficacy was evaluated according to the World Health Organization objective evaluation criteria for tumor efficacy, and the relationship of chemotherapy efficacy with serum MDSC and TRAP1 levels was analyzed. Multivariate Cox regression analysis was conducted to evaluate factors associated with prognosis. The serum levels of TRAP1 and MDSCs were significantly higher in patients with NSCLC than in healthy controls ( P <0.05). Serum TRAP1 and MDSC levels were positively correlated with TNM stage. Serum levels of TRAP1 and MDSCs after chemotherapy were significantly lower than those before chemotherapy in the overall cohort, serum levels of TRAP1 after chemotherapy were significantly lower than those before chemotherapy in patients with partial response, and serum levels of MDSCs after chemotherapy were significantly higher than those before chemotherapy in patients with progressive disease ( P < 0.05). Multivariate Cox regression analysis revealed that high serum levels of TRAP1 and MDSCs were associated with poor prognosis. Serum levels of TRAP1 and MDSCs should be considered as potential predictive biomarkers for chemotherapy efficacy and prognosis in NSCLC.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135349102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Waterproof measures are an important guarantee to protect the tunnel structure and maintain its safe and reliable operation. With the continuous progress of science and technology, waterborne polyurethane (WPU) is widely used in various applications such as coatings and adhesives due to its excellent mechanical properties. With the presence of hydrophilic groups, it is necessary to chemically modify PU due to its reduced performance. The PU prepolymer was prepared using the prepolymer acetone combination method and was emulsified by the high gravity strengthening technology for a better synthetic process. The modified PU composite lotion (CL) was then prepared. When the acrylate content increased, the two parameters, i.e., the size and particle size distribution of PU CL, showed opposite trends. For instance, the size changed from 61.45 nm to 106.5 nm, and the particle size distribution index changed from 0.255 to 0.11. Furthermore, the compressive strength of the material is 13.7 Mpa. When the acrylate content gradually increases, the material tensile strength changes from 11.9 MPa to about 6.5 MPa, and the overall elongation at break remains within 200%–250%. In the actual test of tunnel waterproofing (TW), the water absorption rate of the modified polyurethane changes slightly and is generally kept in the range of 4%–4.5%. It has a good waterproof effect, providing a new method reference for PU CL modification and TW.
{"title":"Performance study and tunnel waterproofing application of polyurethane prepolymer prepared by prepolymer and acetone combination","authors":"Hui Yang, Qiao He, Dongdong Zhang","doi":"10.1166/mex.2023.2500","DOIUrl":"https://doi.org/10.1166/mex.2023.2500","url":null,"abstract":"Waterproof measures are an important guarantee to protect the tunnel structure and maintain its safe and reliable operation. With the continuous progress of science and technology, waterborne polyurethane (WPU) is widely used in various applications such as coatings and adhesives due to its excellent mechanical properties. With the presence of hydrophilic groups, it is necessary to chemically modify PU due to its reduced performance. The PU prepolymer was prepared using the prepolymer acetone combination method and was emulsified by the high gravity strengthening technology for a better synthetic process. The modified PU composite lotion (CL) was then prepared. When the acrylate content increased, the two parameters, i.e., the size and particle size distribution of PU CL, showed opposite trends. For instance, the size changed from 61.45 nm to 106.5 nm, and the particle size distribution index changed from 0.255 to 0.11. Furthermore, the compressive strength of the material is 13.7 Mpa. When the acrylate content gradually increases, the material tensile strength changes from 11.9 MPa to about 6.5 MPa, and the overall elongation at break remains within 200%–250%. In the actual test of tunnel waterproofing (TW), the water absorption rate of the modified polyurethane changes slightly and is generally kept in the range of 4%–4.5%. It has a good waterproof effect, providing a new method reference for PU CL modification and TW.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135348043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this experiment, we explored the role of astragaloside in regulating Egr-1 through S14G-humanin on asthmatic airway inflammation. 64 juvenile Sprague Dawley (SD) rats were selected. After establishing rat asthma model, they were assigned into blank control group, astragaloside group, S14G-Humanin group and astragaloside+S14G-Humanin group (combined group). Astragaloside group was intervened with astragaloside II 0.6 mg/kg, S14G-Humanin group was intervened with 50 μ m S14G-Humanin, combined group RBSMCs were treated with astragaloside II 0.6 mg/kg and 50 μ M S14G-Humaninn. Airway responsiveness was assessed and pathological damage of lung tissue was assessed by HE staining along with analysis of inflammatory cells in bronchoalveolar lavage fluid (BALF), inflammatory cytokines and bone-marrow derived mesenchymal stem cells (rBMSCs) behaviors. Compared to blank control, the Penh values of astragaloside group, S14G-Humanin group and combination group were increased ( P <0.05) and pathological scores were lower with the lowest score in combined group (all P <0.05). The number of white blood cells, neutrophils, eosinophils, macrophages and lymphocytes in BALF of rats in astragaloside group, S14G-Humanin group and combination group were decreased, with the lowest number in combination group ( P <0.05). In addition, IL-4, IL-6, and IL-21 in astragaloside group, S14G-Humanin group and combination group were reduced, with the lowest levels in combination group ( P <0.05). RBSMCs proliferation and migration ability in treatment group was reduced with the lowest in combination group ( P <0.05). After up-regulating S14G-Humanin, Egr-1 mRNA expression was elevated ( P <0.05). Astragaloside can reduce inflammatory cells and inflammatory cytokines and increase the expression of Egr-1 by regulating S14G-Humanin expression.
{"title":"Astragaloside improves asthmatic airway inflammation mediated by inhibition of early growth response-1 through S14G-humanin","authors":"Shengnan Zhou, Youlun Li","doi":"10.1166/mex.2023.2492","DOIUrl":"https://doi.org/10.1166/mex.2023.2492","url":null,"abstract":"In this experiment, we explored the role of astragaloside in regulating Egr-1 through S14G-humanin on asthmatic airway inflammation. 64 juvenile Sprague Dawley (SD) rats were selected. After establishing rat asthma model, they were assigned into blank control group, astragaloside group, S14G-Humanin group and astragaloside+S14G-Humanin group (combined group). Astragaloside group was intervened with astragaloside II 0.6 mg/kg, S14G-Humanin group was intervened with 50 μ m S14G-Humanin, combined group RBSMCs were treated with astragaloside II 0.6 mg/kg and 50 μ M S14G-Humaninn. Airway responsiveness was assessed and pathological damage of lung tissue was assessed by HE staining along with analysis of inflammatory cells in bronchoalveolar lavage fluid (BALF), inflammatory cytokines and bone-marrow derived mesenchymal stem cells (rBMSCs) behaviors. Compared to blank control, the Penh values of astragaloside group, S14G-Humanin group and combination group were increased ( P <0.05) and pathological scores were lower with the lowest score in combined group (all P <0.05). The number of white blood cells, neutrophils, eosinophils, macrophages and lymphocytes in BALF of rats in astragaloside group, S14G-Humanin group and combination group were decreased, with the lowest number in combination group ( P <0.05). In addition, IL-4, IL-6, and IL-21 in astragaloside group, S14G-Humanin group and combination group were reduced, with the lowest levels in combination group ( P <0.05). RBSMCs proliferation and migration ability in treatment group was reduced with the lowest in combination group ( P <0.05). After up-regulating S14G-Humanin, Egr-1 mRNA expression was elevated ( P <0.05). Astragaloside can reduce inflammatory cells and inflammatory cytokines and increase the expression of Egr-1 by regulating S14G-Humanin expression.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135349275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jingjing Li, Danni Zhu, Weichen Zhang, Songmei Tao, Guanghui Fan
Coronary artery disease (CAD) is a common cardiovascular disease that is characterized by accumulation of fatty deposits. Recombinant Human Proline-Rich Acidic Protein 1 (PRAP1) expression is related to prognosis of patients after percutaneous coronary intervention (PCI). This study will be aimed at the treatment by the traditional chinese medicine Sini Decoction (SND) and Siwu Decoction (SWD), and investigate the influence of kaempferol in dried ginger on the postoperative coronary PCI, and further explore the mechanism of kaempferol on the expression of Proline-rich acidic protein 1 (PRAP1) after coronary PCI. Mesenchymal stem cells (MSCs) were isolated and induced to differentiate into endothelial progenitor cells (EPCs). After identification of EPCs by immunofluorescence and angiogenesis assay, cells were divided into high concentration of SND combined with SWD group, low concentration group, vehicle group, and negative control group. Immunofluorescence and Western blot were used to determine the expressions of β -catenin and GSK-3 β as well as PRAP1 in EPCs, whilst cell migration, proliferation and adhesion abilities were assessed. MSCs were positive for CD105 and negative for CD34 and CD45, followed by identification of EPCs with staining. Regardless of concentration, administration of SND plus SWD significantly increased EPC migration and proliferation, but decreased adhesion rate of EPCs ( P <0.05). Moreover, high concentration of SND and SWD significantly facilitated EPC growth and reduced cell adhesion ( P <0.05). Importantly, the levels of PRAP1 and GSK-3 β were elevated, and β -catenin decreased in the presence of SND and SWD, with high concentration achieving more significant alterations than low concentration. EPCs were fluorescently stained and showed proliferative properties and in vitro angiogenesis. Sini Decoction and Siwu Decoction can significantly increase β -catenin expression and decrease GSK-3 β and PRAP1 expression after PCI. Sini Decoction and Siwu Decoction can also promote cell migration and cell proliferation, and significantly reduce the adhesion ability of EPCs, so as to increase new blood vessels, improve cardiac function and protect the heart.
{"title":"The mechanism of influence of kaempferol on proline-rich acidic protein 1 (PRAP1) expression after percutaneous coronary intervention (PCI) was investigated based on sini decoction and siwu decoction","authors":"Jingjing Li, Danni Zhu, Weichen Zhang, Songmei Tao, Guanghui Fan","doi":"10.1166/mex.2023.2493","DOIUrl":"https://doi.org/10.1166/mex.2023.2493","url":null,"abstract":"Coronary artery disease (CAD) is a common cardiovascular disease that is characterized by accumulation of fatty deposits. Recombinant Human Proline-Rich Acidic Protein 1 (PRAP1) expression is related to prognosis of patients after percutaneous coronary intervention (PCI). This study will be aimed at the treatment by the traditional chinese medicine Sini Decoction (SND) and Siwu Decoction (SWD), and investigate the influence of kaempferol in dried ginger on the postoperative coronary PCI, and further explore the mechanism of kaempferol on the expression of Proline-rich acidic protein 1 (PRAP1) after coronary PCI. Mesenchymal stem cells (MSCs) were isolated and induced to differentiate into endothelial progenitor cells (EPCs). After identification of EPCs by immunofluorescence and angiogenesis assay, cells were divided into high concentration of SND combined with SWD group, low concentration group, vehicle group, and negative control group. Immunofluorescence and Western blot were used to determine the expressions of β -catenin and GSK-3 β as well as PRAP1 in EPCs, whilst cell migration, proliferation and adhesion abilities were assessed. MSCs were positive for CD105 and negative for CD34 and CD45, followed by identification of EPCs with staining. Regardless of concentration, administration of SND plus SWD significantly increased EPC migration and proliferation, but decreased adhesion rate of EPCs ( P <0.05). Moreover, high concentration of SND and SWD significantly facilitated EPC growth and reduced cell adhesion ( P <0.05). Importantly, the levels of PRAP1 and GSK-3 β were elevated, and β -catenin decreased in the presence of SND and SWD, with high concentration achieving more significant alterations than low concentration. EPCs were fluorescently stained and showed proliferative properties and in vitro angiogenesis. Sini Decoction and Siwu Decoction can significantly increase β -catenin expression and decrease GSK-3 β and PRAP1 expression after PCI. Sini Decoction and Siwu Decoction can also promote cell migration and cell proliferation, and significantly reduce the adhesion ability of EPCs, so as to increase new blood vessels, improve cardiac function and protect the heart.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135349286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Modern science and technology development has put forward high requirements for armor performance. In this study, a coating based on nanocrystalline copper was proposed to further improve the mechanical properties of the coating and prolong the effective action time of the jet. Equal-diameter angular extrusion was adopted for the grain refinement of pure copper. Cold rolling was performed to strengthen the mechanical properties of nanocrystalline copper, laying a foundation for the follow-up work. The manufacturing process of the drug-type cover was then optimized and improved, and the two-phase theory of jet penetration was introduced to design and analyze the process in detail. Finally, simulation experiments were conducted to analyze the mechanical properties of nanocrystalline copper and the properties of the penetration process. Nanocrystalline copper had significantly improved tensile strength, yield strength, and other properties than the original material. In particular, the tensile strength increased to 195 and 208 MPa in two directions. In the penetration simulation experiment, the shaped charge performance of nanocrystalline copper increased by about 25% compared with that of the original material. Therefore, the nanocrystalline copper synthesized by equal-diameter angle extrusion can improve the jet armor-breaking performance.
{"title":"Application of nanomaterials in improving the nail-breaking performance of metal jets","authors":"Qingyu Meng, Feng Han, Bonan Jiang","doi":"10.1166/mex.2023.2498","DOIUrl":"https://doi.org/10.1166/mex.2023.2498","url":null,"abstract":"Modern science and technology development has put forward high requirements for armor performance. In this study, a coating based on nanocrystalline copper was proposed to further improve the mechanical properties of the coating and prolong the effective action time of the jet. Equal-diameter angular extrusion was adopted for the grain refinement of pure copper. Cold rolling was performed to strengthen the mechanical properties of nanocrystalline copper, laying a foundation for the follow-up work. The manufacturing process of the drug-type cover was then optimized and improved, and the two-phase theory of jet penetration was introduced to design and analyze the process in detail. Finally, simulation experiments were conducted to analyze the mechanical properties of nanocrystalline copper and the properties of the penetration process. Nanocrystalline copper had significantly improved tensile strength, yield strength, and other properties than the original material. In particular, the tensile strength increased to 195 and 208 MPa in two directions. In the penetration simulation experiment, the shaped charge performance of nanocrystalline copper increased by about 25% compared with that of the original material. Therefore, the nanocrystalline copper synthesized by equal-diameter angle extrusion can improve the jet armor-breaking performance.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135348040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study regulated the induced differentiation of bone marrow stromal cells (BMSCs) in rats after anesthesia and explored its effect on nerve repair. The effect of MSC-induced nerve repair was analyzed. The scores of cell-intervention group (5.43± 1.35), nano-intervention group (4.43± 1.36) and nano-+cell-intervention group (4.45±1.49) were significantly lower on 28th day than control group (9.99±1.40), among which, the nano+cell intervention group had lowest score ( P <0.05).The cell intervention (11.35±1.23), nanometer intervention (14.81±1.55) and nano+cell intervention groups (15.96±1.45) had significantly lower score than control group (6.42± 1.46), with lowest score in the nano+ cell intervention group ( P < 0.05). The expressions of glial fibrillary acidic protein (GFAP) and NeuN proteins in the treatment group were significantly decreased, with lowest expression in the nano+cell intervention group ( P <0.05). Average optical density of bFGF and EGF after treatment was significantly elevated, with highest density values in the nano+cell intervention group ( P <0.05). Using retinoic acid polymeric nanoparticles to regulate MSCs differentiation can make retinoic acid bind to neuronal receptors, promoting axon growth, and improving nerve function and motor function. It can reduce downregulate GFAP and NeuN, increase the bFGF and EGF level, which can be used as a new target marker. With the deepening research on nanoparticles, retinoic acid nanoparticles will have broad application prospects.
{"title":"Polymerized retinoic acid nanoparticles modulate neurorestorative effects induced by bone marrow stromal cells in rats after anesthesia","authors":"Xiu Qu, Feng Run, Hua Yu","doi":"10.1166/mex.2023.2494","DOIUrl":"https://doi.org/10.1166/mex.2023.2494","url":null,"abstract":"This study regulated the induced differentiation of bone marrow stromal cells (BMSCs) in rats after anesthesia and explored its effect on nerve repair. The effect of MSC-induced nerve repair was analyzed. The scores of cell-intervention group (5.43± 1.35), nano-intervention group (4.43± 1.36) and nano-+cell-intervention group (4.45±1.49) were significantly lower on 28th day than control group (9.99±1.40), among which, the nano+cell intervention group had lowest score ( P <0.05).The cell intervention (11.35±1.23), nanometer intervention (14.81±1.55) and nano+cell intervention groups (15.96±1.45) had significantly lower score than control group (6.42± 1.46), with lowest score in the nano+ cell intervention group ( P < 0.05). The expressions of glial fibrillary acidic protein (GFAP) and NeuN proteins in the treatment group were significantly decreased, with lowest expression in the nano+cell intervention group ( P <0.05). Average optical density of bFGF and EGF after treatment was significantly elevated, with highest density values in the nano+cell intervention group ( P <0.05). Using retinoic acid polymeric nanoparticles to regulate MSCs differentiation can make retinoic acid bind to neuronal receptors, promoting axon growth, and improving nerve function and motor function. It can reduce downregulate GFAP and NeuN, increase the bFGF and EGF level, which can be used as a new target marker. With the deepening research on nanoparticles, retinoic acid nanoparticles will have broad application prospects.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135349103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Clinical studies have shown that, ginsenoside Rg3 has strong antitumor and antioxidant effects. Therefore, this study used ginsenoside Rg3 to explore its role in the antioxidant response element (ARE) signaling pathway, to clarify the mechanism of regulating superoxide dismutase (SOD) and enhancing sensitivity of cancer cells to photodynamic therapy, providing a basis for improving clinical treatment effect. A papillary thyroid carcinoma mouse model was constructed and divided into study groups, followed by Ki-67 and TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, CCK-8 method and flow cytometry analysis. Level of reactive oxygen species, and ARE and SOD were assessed by qRT-PCR (quantificational rt-PCR) and Western blot. No mouse death occurred during model establishment and intervention, and the tumor formation rate was 100%. Moreover, the Ki-67 positive cells in tumor tissues from the ginsenoside Rg3 group were lowest, indicating tumor growth was inhibited; while the TUNEL cells were increased, indicating that tumor cells underwent apoptosis. Meanwhile, BCPAP (Human Thyroid Cancer Papillary Cell) proliferation and migration in the ginsenoside Rg3 group were lower than in the ARE inhibitor group, while apoptotic ability was increased. The levels of ARE and SOD in the ginsenoside Rg3 group were also increased. Ginsenoside Rg3 plays an anti-tumor effect through ARE signaling pathway and accelerates cell apoptosis. At the same time, the Ginsenoside Rg3 can enhance ROS activity, upregulate ARE and SOD, and increase the sensitivity of cancer cells to photodynamic therapy.
{"title":"Ginsenoside Rg3 regulates sensitization effect of superoxide dismutase on thyroid cancer photodynamic therapy via antioxidant response element signaling pathway","authors":"Yanhui Yin, Qing Li, Yunlong Zhang","doi":"10.1166/mex.2023.2491","DOIUrl":"https://doi.org/10.1166/mex.2023.2491","url":null,"abstract":"Clinical studies have shown that, ginsenoside Rg3 has strong antitumor and antioxidant effects. Therefore, this study used ginsenoside Rg3 to explore its role in the antioxidant response element (ARE) signaling pathway, to clarify the mechanism of regulating superoxide dismutase (SOD) and enhancing sensitivity of cancer cells to photodynamic therapy, providing a basis for improving clinical treatment effect. A papillary thyroid carcinoma mouse model was constructed and divided into study groups, followed by Ki-67 and TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, CCK-8 method and flow cytometry analysis. Level of reactive oxygen species, and ARE and SOD were assessed by qRT-PCR (quantificational rt-PCR) and Western blot. No mouse death occurred during model establishment and intervention, and the tumor formation rate was 100%. Moreover, the Ki-67 positive cells in tumor tissues from the ginsenoside Rg3 group were lowest, indicating tumor growth was inhibited; while the TUNEL cells were increased, indicating that tumor cells underwent apoptosis. Meanwhile, BCPAP (Human Thyroid Cancer Papillary Cell) proliferation and migration in the ginsenoside Rg3 group were lower than in the ARE inhibitor group, while apoptotic ability was increased. The levels of ARE and SOD in the ginsenoside Rg3 group were also increased. Ginsenoside Rg3 plays an anti-tumor effect through ARE signaling pathway and accelerates cell apoptosis. At the same time, the Ginsenoside Rg3 can enhance ROS activity, upregulate ARE and SOD, and increase the sensitivity of cancer cells to photodynamic therapy.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135349108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study explores methodologies for removing heavy metal elements such as nickel (Ni), copper (Cu), cadmium (Cd), and lead (Pb) from diverse aquatic environments, including rivers, lakes, and oceans. Nanosized montmorillonite (MON) was used as the raw material and was subjected to organic chemical modification through silanization using cetyltrimethylammonium bromide and grafting of amino groups to produce amino-functionalized nanomontmorillonite composite (NH 2 -MON). The removal effectiveness of NH 2 -MON on heavy metal elements in water bodies was evaluated. Experiments involving adsorption were conducted to evaluate the impact of nanomaterial concentration and solution pH on the entrapment of heavy metal ions. The results indicated that an increased nanomaterial adsorbent dosage precipitated water coagulation, which subsequently altered the accessibility of adsorption sites for heavy metal ions, thereby significantly affecting the heavy metal removal effectiveness of the nanomaterial. The ideal nanomaterial dosage was determined to be 2.5 g/L, yielding the maximum unit adsorption capacity and removal rate. The acidity or alkalinity of the solution was instrumental in the adsorption of heavy metal ions such as Ni, Cu, Cd, and Pb using nanomaterials, establishing solution pH as a pivotal determinant in the adsorption process. As the solution pH increased, the electronegativity of the nanomaterial increased, thus encouraging its interaction with positively charged heavy metal ions, including Ni, Cu, Cd, and Pb. The ideal solution pH range was found to be 4–5.
{"title":"Detection and remediation of heavy metal pollution in seawater using instrumentation and nanomaterials","authors":"Keming Wang, Chengli Wang, Wenbing Jin, Liuming Qi","doi":"10.1166/mex.2023.2499","DOIUrl":"https://doi.org/10.1166/mex.2023.2499","url":null,"abstract":"This study explores methodologies for removing heavy metal elements such as nickel (Ni), copper (Cu), cadmium (Cd), and lead (Pb) from diverse aquatic environments, including rivers, lakes, and oceans. Nanosized montmorillonite (MON) was used as the raw material and was subjected to organic chemical modification through silanization using cetyltrimethylammonium bromide and grafting of amino groups to produce amino-functionalized nanomontmorillonite composite (NH 2 -MON). The removal effectiveness of NH 2 -MON on heavy metal elements in water bodies was evaluated. Experiments involving adsorption were conducted to evaluate the impact of nanomaterial concentration and solution pH on the entrapment of heavy metal ions. The results indicated that an increased nanomaterial adsorbent dosage precipitated water coagulation, which subsequently altered the accessibility of adsorption sites for heavy metal ions, thereby significantly affecting the heavy metal removal effectiveness of the nanomaterial. The ideal nanomaterial dosage was determined to be 2.5 g/L, yielding the maximum unit adsorption capacity and removal rate. The acidity or alkalinity of the solution was instrumental in the adsorption of heavy metal ions such as Ni, Cu, Cd, and Pb using nanomaterials, establishing solution pH as a pivotal determinant in the adsorption process. As the solution pH increased, the electronegativity of the nanomaterial increased, thus encouraging its interaction with positively charged heavy metal ions, including Ni, Cu, Cd, and Pb. The ideal solution pH range was found to be 4–5.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"371 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135349285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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