Refractory diabetic foot ulcer is commonly encountered in clinical practice and it is hardly cured with a long duration of treatment and a high expense, as such problem involves multiple disciplines. Therefore, in case of refractory wounds, it is necessary to first analyze the causes, general or local. Immunohistochemistry (IHC) was conducted to determine α-SMA, chemokines 12 (CXCL12) and chemokines 4 (CXCR4) levels in pancreatic tissues and distant normal pancreatic tissues. Cells were treated with recombinant human CXCL12 (rhCXCL12) or the CXCR4 antagonist AMD3100. After treatment, Western blot determined FAK-AKT and ERK1/2 expression in islet cells, whilst ELISA detected the content of IL-6 and IL-8. rhCXCL12 increased the expression and secretion of Interleukin-6 (IL-6) time- and dose-dependently. But the advent of CXCR4 antagonist abrogated the protective effect of rhCXCL12. rhCXCL12 exhibited a protective effect on apoptosis, but this effect was abrogated by down-regulation of IL-6 with AMD3100. In addition, rhCXCL12 increased the phosphorylation of FAK, ERK1/2, AKT, and P38 and inhibition of FAK inhibited IL-6 expression. FAK inhibition almost completely blocked CXCL12-induced activation. This study demonstrates that CXCL12/CXCR4 pathway mediates the expression of IL-6 to enhance healing of refractory diabetic foot wounds by down-regulating the FAK pathway.
{"title":"The protective role of chemokines 12 and chemokines 4 by mediating interleukin-6 in delayed diabetic foot wound healing","authors":"Chaoyan Yin, Yuan Lin, Fan Zhang, Xiaofen Lian","doi":"10.1166/mex.2024.2575","DOIUrl":"https://doi.org/10.1166/mex.2024.2575","url":null,"abstract":"Refractory diabetic foot ulcer is commonly encountered in clinical practice and it is hardly cured with a long duration of treatment and a high expense, as such problem involves multiple disciplines. Therefore, in case of refractory wounds, it is necessary to first analyze the causes, general or local. Immunohistochemistry (IHC) was conducted to determine α-SMA, chemokines 12 (CXCL12) and chemokines 4 (CXCR4) levels in pancreatic tissues and distant normal pancreatic tissues. Cells were treated with recombinant human CXCL12 (rhCXCL12) or the CXCR4 antagonist AMD3100. After treatment, Western blot determined FAK-AKT and ERK1/2 expression in islet cells, whilst ELISA detected the content of IL-6 and IL-8. rhCXCL12 increased the expression and secretion of Interleukin-6 (IL-6) time- and dose-dependently. But the advent of CXCR4 antagonist abrogated the protective effect of rhCXCL12. rhCXCL12 exhibited a protective effect on apoptosis, but this effect was abrogated by down-regulation of IL-6 with AMD3100. In addition, rhCXCL12 increased the phosphorylation of FAK, ERK1/2, AKT, and P38 and inhibition of FAK inhibited IL-6 expression. FAK inhibition almost completely blocked CXCL12-induced activation. This study demonstrates that CXCL12/CXCR4 pathway mediates the expression of IL-6 to enhance healing of refractory diabetic foot wounds by down-regulating the FAK pathway.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"4 9","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139124940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the shield tunnel, the phenomenon of structure penetration occurs frequently. Therefore, strict control of ground subsidence is imperative during the construction phase. In addition, other variables may be influence the performance of the slurry during the grouting procedure. This study aims to investigate and analyze the impact of nanomaterials on the properties of slurry used in construction, with the goal of determining the optimal construction route for the shield, ensuring quality construction of a U-shaped section, and meeting settlement control requirements. Firstly, the impact of double line spacing on the subsidence of U-shaped grooves is analyzed. Subsequently, the influence of common nanomaterials on slurry properties is examined through compressive strength testing. Among these nanomaterials, micro-silica powder exhibits the most significant enhancement effect on construction slurry performance with an optimal content of 5%. If the content of silica powder reaches 5%, the initial strength and later strength of the cement slurry will increase by 35% and 25% respectively, thereby promoting the enhanced performance of the slurry. In this study, the settlement of a U-shaped groove on the surface and subgrade is controlled by the settlement of a shield tunnel underpass railway model based on the distance between shield construction lines, providing valuable insights for future constructions with similar characteristics.
在盾构隧道中，结构贯穿现象经常发生。因此，在施工阶段必须严格控制地面沉降。此外，在注浆过程中，其他变量也可能影响浆液的性能。本研究旨在调查和分析纳米材料对施工所用泥浆性能的影响，目的是确定盾构的最佳施工路线，确保 U 型断面的施工质量，满足沉降控制要求。 首先，分析了双线间距对 U 型槽沉降的影响。随后，通过抗压强度测试研究了常见纳米材料对泥浆性能的影响。在这些纳米材料中，微硅粉对建筑泥浆性能的增强效果最为显著，最佳含量为 5%。如果二氧化硅粉的含量达到 5%，水泥浆的初始强度和后期强度将分别提高 35% 和 25%，从而促进水泥浆性能的提高。 在这项研究中，根据盾构施工线之间的距离，通过盾构隧道下穿铁路模型的沉降来控制地表和路基上 U 形槽的沉降，为今后具有类似特征的施工提供了有价值的启示。
{"title":"Settlement rule of underpass railway model in shield tunnel and influence of nanomaterials on slurry performance during construction","authors":"Ying Li, Minji Wang, Yawen Yang, Haoran Guo, Yilin Zhou","doi":"10.1166/mex.2024.2582","DOIUrl":"https://doi.org/10.1166/mex.2024.2582","url":null,"abstract":"In the shield tunnel, the phenomenon of structure penetration occurs frequently. Therefore, strict control of ground subsidence is imperative during the construction phase. In addition, other variables may be influence the performance of the slurry during the grouting procedure. This study aims to investigate and analyze the impact of nanomaterials on the properties of slurry used in construction, with the goal of determining the optimal construction route for the shield, ensuring quality construction of a U-shaped section, and meeting settlement control requirements. Firstly, the impact of double line spacing on the subsidence of U-shaped grooves is analyzed. Subsequently, the influence of common nanomaterials on slurry properties is examined through compressive strength testing. Among these nanomaterials, micro-silica powder exhibits the most significant enhancement effect on construction slurry performance with an optimal content of 5%. If the content of silica powder reaches 5%, the initial strength and later strength of the cement slurry will increase by 35% and 25% respectively, thereby promoting the enhanced performance of the slurry. In this study, the settlement of a U-shaped groove on the surface and subgrade is controlled by the settlement of a shield tunnel underpass railway model based on the distance between shield construction lines, providing valuable insights for future constructions with similar characteristics.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"26 21","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dong Xie, Gang Zhou, Wei Zhang, Y. Gao, Xing Cai, Yang Cai, Zhiyun Liu, Weijun Zhao
Lung cancer treatment is still based on chemotherapy. Autophagy involves in lung cancer. Our research aims to explore miR-135’s role in lung cancer. Tumor tissues were collected for analysis of autophagy. TargetScan bioinformatics assessed the relationship between miR-135 and ATG7. Immunofluorescence analyzed the co-localization of circRACGAP1 and miR-135. circRACGAP1 was silenced to assess its role in autophagy and lung cancer cell growth. In A549 cells, cisplatin combined with pemetrexed upregulated ATG7 and LC3-II, and downregulated squestosome 1 (SQSTM1). Significantly upregulated LC3-II and ATG7 and reduced SQSTM1 were found in cisplatin combined with pemetrexed group. miR-135 targeted ATG7 gene 3′-UTR region. Cisplatin combined with pemetrexed upregulated ATG7 by selectively inhibiting miR-135. circRACGAP1 was co-localized with miR-135. circRACGAP1 inhibition decreased lung cancer cell growth by inhibiting autophagy. circRACGAP1-miR-135-ATG7 axis involves in the regulatory effect of cisplatin combined with pemetrexed on lung cancer cell growth.
{"title":"The inhibitory effect of cisplatin combined with pemetrexed on lung cancer cells via targeting miR-135 and ATG7","authors":"Dong Xie, Gang Zhou, Wei Zhang, Y. Gao, Xing Cai, Yang Cai, Zhiyun Liu, Weijun Zhao","doi":"10.1166/mex.2024.2588","DOIUrl":"https://doi.org/10.1166/mex.2024.2588","url":null,"abstract":"Lung cancer treatment is still based on chemotherapy. Autophagy involves in lung cancer. Our research aims to explore miR-135’s role in lung cancer. Tumor tissues were collected for analysis of autophagy. TargetScan bioinformatics assessed the relationship between miR-135 and ATG7. Immunofluorescence analyzed the co-localization of circRACGAP1 and miR-135. circRACGAP1 was silenced to assess its role in autophagy and lung cancer cell growth. In A549 cells, cisplatin combined with pemetrexed upregulated ATG7 and LC3-II, and downregulated squestosome 1 (SQSTM1). Significantly upregulated LC3-II and ATG7 and reduced SQSTM1 were found in cisplatin combined with pemetrexed group. miR-135 targeted ATG7 gene 3′-UTR region. Cisplatin combined with pemetrexed upregulated ATG7 by selectively inhibiting miR-135. circRACGAP1 was co-localized with miR-135. circRACGAP1 inhibition decreased lung cancer cell growth by inhibiting autophagy. circRACGAP1-miR-135-ATG7 axis involves in the regulatory effect of cisplatin combined with pemetrexed on lung cancer cell growth.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"107 17","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139128668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sisi Xie, Wenze Dong, Wenming Wu, Yanxin Xie, Fangfang Du
Explore the effect of surface scratches on material properties, Two analytical models for thermal damage were established. The modulated light field generated by scratches at different relative positions was calculated. By analyzing the distribution of surface temperatures, we delve into the thermal damage thresholds of SiO2. Results show that, for parallel scratches, the thermal damage threshold will vary with the interval of the scratches, the damage threshold also affected by the depth-width ratio of the scratch. For cross-scratches defects, both the cross angle and depth-width ratio have effects on the thermal damage threshold. And for the two kinds scratches the depth-width ratio is easier to make the material damaged.
{"title":"Effects of surface scratches on thermal damage characteristics of SiO2","authors":"Sisi Xie, Wenze Dong, Wenming Wu, Yanxin Xie, Fangfang Du","doi":"10.1166/mex.2024.2598","DOIUrl":"https://doi.org/10.1166/mex.2024.2598","url":null,"abstract":"Explore the effect of surface scratches on material properties, Two analytical models for thermal damage were established. The modulated light field generated by scratches at different relative positions was calculated. By analyzing the distribution of surface temperatures, we delve\u0000 into the thermal damage thresholds of SiO2. Results show that, for parallel scratches, the thermal damage threshold will vary with the interval of the scratches, the damage threshold also affected by the depth-width ratio of the scratch. For cross-scratches defects, both the cross\u0000 angle and depth-width ratio have effects on the thermal damage threshold. And for the two kinds scratches the depth-width ratio is easier to make the material damaged.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"29 4","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139395141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigates the effect of long-chain non-coding LINC00657 on cell proliferation and apoptosis in colorectal cancer and its molecular mechanism based on magnetic nanocarriers for gene delivery. Tumor tissues were collected from patients with colorectal cancer (CRC) to examine the expression of LINC00657. Magnetic nano microspheres were prepared and CRC cells were transfected with short hairpin RNA (shRNA) targeting LINC00657 to establish a stably transfected cell line. The binding relationship between LINC00657 and MicroRNA-26b (MiR-26b) was also verified using a dual-luciferase gene reporter assay. The expression of LINC00657 and MiR-26b were determined by qRT-PCR and Western blot. Cell proliferation, migration, and apoptosis were assessed. Our findings reveal that LINC00657 is highly expressed in CRC tissues and cells, significantly promoting hepatoma carcinoma cell proliferation and migration. Furthermore, we demonstrate that MiR-26b can target and bind to LINC00657, while its expression decreases in CRC cells. Knockdown of LINC00657 resulted in significant downregulation of both MiR-26b expression levels and tumor necrosis factor-α (TNF-α)/nuclear factor-kappa B (NF-κB) pathway activity. However, co-transfection of a MiR-26b inhibitor into sh-LINC00657-transfected cells attenuated the effects on malignant phenotypes while activating TNF-α/NF-kB pathway activity. Conversely, transfection of an NF-kB activator exerted similar effects as the MiR-26b inhibitor by enhancing malignant proliferation ability in CRC cells. Overall, the upregulation of LINC00657 potentially modulates the proliferation and apoptosis of colorectal cancer cells through its interaction with MiR-26b, leading to the activation of the TNF-α/NF-κB signaling pathway.
{"title":"Regulatory mechanism of LINC00657/MiR-26b in the TNF-α/NF-κB pathway for the progression of colorectal cancer","authors":"Shengjun Zhang, Minli Liu, Ajing Zhao, Anrui Zhang, Yawei Li, Xiaobao Li","doi":"10.1166/mex.2024.2592","DOIUrl":"https://doi.org/10.1166/mex.2024.2592","url":null,"abstract":"This study investigates the effect of long-chain non-coding LINC00657 on cell proliferation and apoptosis in colorectal cancer and its molecular mechanism based on magnetic nanocarriers for gene delivery. Tumor tissues were collected from patients with colorectal cancer (CRC) to examine the expression of LINC00657. Magnetic nano microspheres were prepared and CRC cells were transfected with short hairpin RNA (shRNA) targeting LINC00657 to establish a stably transfected cell line. The binding relationship between LINC00657 and MicroRNA-26b (MiR-26b) was also verified using a dual-luciferase gene reporter assay. The expression of LINC00657 and MiR-26b were determined by qRT-PCR and Western blot. Cell proliferation, migration, and apoptosis were assessed. Our findings reveal that LINC00657 is highly expressed in CRC tissues and cells, significantly promoting hepatoma carcinoma cell proliferation and migration. Furthermore, we demonstrate that MiR-26b can target and bind to LINC00657, while its expression decreases in CRC cells. Knockdown of LINC00657 resulted in significant downregulation of both MiR-26b expression levels and tumor necrosis factor-α (TNF-α)/nuclear factor-kappa B (NF-κB) pathway activity. However, co-transfection of a MiR-26b inhibitor into sh-LINC00657-transfected cells attenuated the effects on malignant phenotypes while activating TNF-α/NF-kB pathway activity. Conversely, transfection of an NF-kB activator exerted similar effects as the MiR-26b inhibitor by enhancing malignant proliferation ability in CRC cells. Overall, the upregulation of LINC00657 potentially modulates the proliferation and apoptosis of colorectal cancer cells through its interaction with MiR-26b, leading to the activation of the TNF-α/NF-κB signaling pathway.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"3 5","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guangyu Cao, Jianju Zhou, Guoqing Yu, Xiaohong Fu, Bo Xiong
Stroke is one of the important causes of death of patients. Both butylphthalide sodium chloride injection (BSCI) and edaravone dextroborneol (ED) show a curative effect. We intend to assess their effect on acute stroke. Patients with acute ischemic stroke diagnosed by our hospital in the past were included as research objects and different treatment methods were used to divide them into different groups. The research group was given combined treatment (BSCI and ED). The normal group chose one of the drugs as treatment. The curative effect was recorded. The neurological deficit score, the commonly used clinical infection markers mainly include IL-6, CRP and TNF-α, and the Barthel index widely used in the field of rehabilitation and elderly patients, hemorheology index and the expression of NSE were evaluated. The changes before and after medical level were compared with the adverse reaction group during the treatment. The analysis of Barthel index reflected that the effective rate was higher than normal group. Changes in neural functions were decreased than normal group (P <0.05) and the adverse reactions in the curing process were lower in both groups, but there was no statistical difference (P >0.05). Our research shows that combined therapy can improve drug efficacy by reducing the expression of cytokines and it does not bring about an increase in toxic and side effects. Combination therapy improves neurological function, reduces cytokine secretion, improves drug efficacy, and has a high drug safety.
{"title":"Clinical efficacy and short-term prognosis of butylphthalide sodium chloride injection combined with edaravone dextroborneol in acute stroke","authors":"Guangyu Cao, Jianju Zhou, Guoqing Yu, Xiaohong Fu, Bo Xiong","doi":"10.1166/mex.2024.2586","DOIUrl":"https://doi.org/10.1166/mex.2024.2586","url":null,"abstract":"Stroke is one of the important causes of death of patients. Both butylphthalide sodium chloride injection (BSCI) and edaravone dextroborneol (ED) show a curative effect. We intend to assess their effect on acute stroke. Patients with acute ischemic stroke diagnosed by our hospital in the past were included as research objects and different treatment methods were used to divide them into different groups. The research group was given combined treatment (BSCI and ED). The normal group chose one of the drugs as treatment. The curative effect was recorded. The neurological deficit score, the commonly used clinical infection markers mainly include IL-6, CRP and TNF-α, and the Barthel index widely used in the field of rehabilitation and elderly patients, hemorheology index and the expression of NSE were evaluated. The changes before and after medical level were compared with the adverse reaction group during the treatment. The analysis of Barthel index reflected that the effective rate was higher than normal group. Changes in neural functions were decreased than normal group (P <0.05) and the adverse reactions in the curing process were lower in both groups, but there was no statistical difference (P >0.05). Our research shows that combined therapy can improve drug efficacy by reducing the expression of cytokines and it does not bring about an increase in toxic and side effects. Combination therapy improves neurological function, reduces cytokine secretion, improves drug efficacy, and has a high drug safety.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"57 4","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Metallothionein (MT1M) is associated with tumors and autoimmune diseases. However, its role in DR has not yet been elucidated. DR and normal rat retinal endothelial cells (RECs) were isolated and cultured. DR Rat RECs achieved gene regulation by transfecting MT1M plasmid. PCR and MTT were used to detect MT1M expression, cell proliferation. Flow cytometry was used to detect cell apoptosis. Lactate dehydrogenase (LDH), superoxide dismutase (SOD) and reactive oxygen species (ROS) were detected by MTT. The expression of VEGF and PI3K/AKT signaling pathway was detected by Western blot. The levels of inflammatory factors TNF-α and IL-1β were detected by ELISA. The results showed that the expression of MT1M was reduced in the RECs of DRC rats compared to the normal control group, cell proliferation was enhanced, SOD activity was reduced, LDH and ROS levels were increased, TNF-α and IL-1β secretion increased, and vascular endothelial growth factor (VEGF), PI3K/AKT expression increased (P < 0.05). However, transfection with MT1M plasmid could significantly inhibit cell proliferation, increase SOD activity, reduce LDH and ROS levels, reduce TNF-α and IL-1β secretion, and reduce VEGF and PI3K/AKT expression (P <0.05). The expression of MT1M is reduced in RECs of DR rats. Up-regulation of MT1M can regulate DR3K/AKT signaling pathway and oxidative/antioxidant balance, alter VEGF expression, inhibit inflammation, regulate the growth and proliferation of RECs, and delay DR lesions.
{"title":"The mechanism of metallothionein MT1M-mediated PI3K/AKT signaling pathway in the regulation of diabetic retinopathy","authors":"Lu Gao, Fangling Song, Ting Liu","doi":"10.1166/mex.2024.2594","DOIUrl":"https://doi.org/10.1166/mex.2024.2594","url":null,"abstract":"Metallothionein (MT1M) is associated with tumors and autoimmune diseases. However, its role in DR has not yet been elucidated. DR and normal rat retinal endothelial cells (RECs) were isolated and cultured. DR Rat RECs achieved gene regulation by transfecting MT1M plasmid. PCR and MTT were used to detect MT1M expression, cell proliferation. Flow cytometry was used to detect cell apoptosis. Lactate dehydrogenase (LDH), superoxide dismutase (SOD) and reactive oxygen species (ROS) were detected by MTT. The expression of VEGF and PI3K/AKT signaling pathway was detected by Western blot. The levels of inflammatory factors TNF-α and IL-1β were detected by ELISA. The results showed that the expression of MT1M was reduced in the RECs of DRC rats compared to the normal control group, cell proliferation was enhanced, SOD activity was reduced, LDH and ROS levels were increased, TNF-α and IL-1β secretion increased, and vascular endothelial growth factor (VEGF), PI3K/AKT expression increased (P < 0.05). However, transfection with MT1M plasmid could significantly inhibit cell proliferation, increase SOD activity, reduce LDH and ROS levels, reduce TNF-α and IL-1β secretion, and reduce VEGF and PI3K/AKT expression (P <0.05). The expression of MT1M is reduced in RECs of DR rats. Up-regulation of MT1M can regulate DR3K/AKT signaling pathway and oxidative/antioxidant balance, alter VEGF expression, inhibit inflammation, regulate the growth and proliferation of RECs, and delay DR lesions.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"58 5","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Our study assesses miR-760’s role in the proliferation, invasion and migration of non-small cell lung cancer (NSCLC) cells. Lung cancer A549 cells were assigned into BL group (no transfection), ZN group (transfection of NC), and ZM group (transfection of miR-760 mimics). The miR-760 level, cell viability, apoptosis, invasion, migration ability, ERK1, JNK, and p38MAPK protein expression were analyzed using Real Time Quantitative polymerase chain reaction (RT-qPCR), MTT, Hoechst33258 fluorescence staining, Transwell cell, cell scratch test, and Western blot. Compared with other groups, miR-760 expression in ZM group was the highest, indicating a successful transfection (P <0.05). 48, 72, and 96 hours later, A549 cell viability in ZM group was the lowest with a significant difference from other groups (P <0.05). The cell viability reached a peak after 96 hours of culture (P <0.05) with higher cell viability of BL group than ZN group (P >0.05). A549 cells in ZM group showed significantly higher cell apoptosis rate and lower cell invasion rate than ZN and BL group (P <0.05). The number of cell invasion (220.71±15.37) and migration in BL group (220.37±17.60) is similar to ZN group (215.32±15.62 and 217.26±15.94) (P >0.05). In addition, cell migration number in ZM group (99.62±12.01) was less than ZN and BL group (P <0.05). ERK1, JNK and p38MAPK protein expression in ZM group was the lowest (P <0.05) and those in BL group was close to ZN group (P >0.05). Upregulating miR-760 in A549 cancer cells can inhibit cell proliferation via regulation of MAPK signaling pathway.
{"title":"miR-760 inhibits lung cancer cells through mitogen-activated protein kinase signaling pathway","authors":"Jiao He, Yaolan Zhen, Lei Liu","doi":"10.1166/mex.2024.2587","DOIUrl":"https://doi.org/10.1166/mex.2024.2587","url":null,"abstract":"Our study assesses miR-760’s role in the proliferation, invasion and migration of non-small cell lung cancer (NSCLC) cells. Lung cancer A549 cells were assigned into BL group (no transfection), ZN group (transfection of NC), and ZM group (transfection of miR-760 mimics). The miR-760 level, cell viability, apoptosis, invasion, migration ability, ERK1, JNK, and p38MAPK protein expression were analyzed using Real Time Quantitative polymerase chain reaction (RT-qPCR), MTT, Hoechst33258 fluorescence staining, Transwell cell, cell scratch test, and Western blot. Compared with other groups, miR-760 expression in ZM group was the highest, indicating a successful transfection (P <0.05). 48, 72, and 96 hours later, A549 cell viability in ZM group was the lowest with a significant difference from other groups (P <0.05). The cell viability reached a peak after 96 hours of culture (P <0.05) with higher cell viability of BL group than ZN group (P >0.05). A549 cells in ZM group showed significantly higher cell apoptosis rate and lower cell invasion rate than ZN and BL group (P <0.05). The number of cell invasion (220.71±15.37) and migration in BL group (220.37±17.60) is similar to ZN group (215.32±15.62 and 217.26±15.94) (P >0.05). In addition, cell migration number in ZM group (99.62±12.01) was less than ZN and BL group (P <0.05). ERK1, JNK and p38MAPK protein expression in ZM group was the lowest (P <0.05) and those in BL group was close to ZN group (P >0.05). Upregulating miR-760 in A549 cancer cells can inhibit cell proliferation via regulation of MAPK signaling pathway.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"20 4","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139128189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alaa Baazeem, M. Helal, R. Sami, G. Alshehry, Eman Algarni, Uguru Hilary, Fadi Baakdah, S. Alharthy, Doaa Mahmoud Johari
Dietary honey as a natural product can attenuate the inflammatory process and prevent several diseases. The current research aimed to assess some positive effects of dietary honey and aflatoxin B1 on serum enzymes, superoxide dismutase activity, β-glucuronidase enzyme activity, and colonic probiotic bacteria in rats. Four kinds of honey were coded as NSH, MOH, SIH, and PUH for Nigella sativa, moringa, pumpkin, and Sidr honey, respectively. Aflatoxin B1 (AFB1) was daily administered orally for rats with a dose of 200 μg/kg until 90 days. The rat groups’ body weights at the beginning and end of the experiment ranged from 311.29 g to 327.55 g, and 302.97 g to 342.77 g respectively. Dietary honey decreased the values of some serum enzymes and protected the animals from AFB1 hepatotoxicity compared with the positive group (+). The concentrations of liver superoxide dismutase activity (SOD) among rat groups ranged from 19.55 u ·g in MOH+AFB1 rat group to 27.21 u·g in SIH+AFB1 rat group. Dietary honey was reported to defend the liver against aflatoxins and enhance the gut microflora as β-glucuronidase activity and colonic probiotic bacteria in rats.
{"title":"Positive effects of dietary honey and aflatoxin B1 on serum enzymes, superoxide dismutase activity, β-glucuronidase enzyme activity, and colonic probiotic bacteria on rats","authors":"Alaa Baazeem, M. Helal, R. Sami, G. Alshehry, Eman Algarni, Uguru Hilary, Fadi Baakdah, S. Alharthy, Doaa Mahmoud Johari","doi":"10.1166/mex.2024.2584","DOIUrl":"https://doi.org/10.1166/mex.2024.2584","url":null,"abstract":"Dietary honey as a natural product can attenuate the inflammatory process and prevent several diseases. The current research aimed to assess some positive effects of dietary honey and aflatoxin B1 on serum enzymes, superoxide dismutase activity, β-glucuronidase enzyme activity, and colonic probiotic bacteria in rats. Four kinds of honey were coded as NSH, MOH, SIH, and PUH for Nigella sativa, moringa, pumpkin, and Sidr honey, respectively. Aflatoxin B1 (AFB1) was daily administered orally for rats with a dose of 200 μg/kg until 90 days. The rat groups’ body weights at the beginning and end of the experiment ranged from 311.29 g to 327.55 g, and 302.97 g to 342.77 g respectively. Dietary honey decreased the values of some serum enzymes and protected the animals from AFB1 hepatotoxicity compared with the positive group (+). The concentrations of liver superoxide dismutase activity (SOD) among rat groups ranged from 19.55 u ·g in MOH+AFB1 rat group to 27.21 u·g in SIH+AFB1 rat group. Dietary honey was reported to defend the liver against aflatoxins and enhance the gut microflora as β-glucuronidase activity and colonic probiotic bacteria in rats.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"27 10","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this experimental protocol, we scrutinized the preventive effect of Kudzu vine extract on DOX-induced cardiac toxicity via alteration of gut microbiota. Sprague-Dawley (SD) was used in this protocol and the rats were divided into different groups and received the DOX (3 mg/kg) for induction the cardiac remodeling and Kudzu vine extract (50, 100 and 200 mg/kg) administration was used for the estimation of cardioprotective potential for 5 weeks. Afterward, biochemical parameter, electrolyte, antioxidant, cytokines and inflammatory parameters were estimated. Kudzu vine remarkably improved the body weight and declined the heart weight. Kudzu vine extract significantly (P <0.001) suppressed the level of CK-MB, LDH, CK, cTnT and BNP. Kudzu vine significantly (P <0.001) improved the level of Na+, Ca2+, K+ and Cl− and decreased the level of urea, magnesium, total protein, albumin and globulin. Kudzu vine remarkably altered the lipid and antioxidant parameters. Additionally, Kudzu vine extract suppressed the level of TNF-α, IL-1β, IL-6 and improved the level of TGF-β, IL-10. DOX-induced cardiac dysfunction exhibited the altered relative abundance of different bacteria and Kudzu vine remarkably restore the relative abundance of different bacteria. Therefore, Kudzu vine extract has beneficial effects against DOX-induced cardiac toxicity via alteration of gut microbiota.
{"title":"Preparation of Kudzu vine oral formulation and its application against doxorubicin-induced cardiotoxicity via alteration of gut microbiota","authors":"Qingmei Wang, Xuanguo Zhang","doi":"10.1166/mex.2024.2533","DOIUrl":"https://doi.org/10.1166/mex.2024.2533","url":null,"abstract":"In this experimental protocol, we scrutinized the preventive effect of Kudzu vine extract on DOX-induced cardiac toxicity via alteration of gut microbiota. Sprague-Dawley (SD) was used in this protocol and the rats were divided into different groups and received the DOX (3 mg/kg) for induction the cardiac remodeling and Kudzu vine extract (50, 100 and 200 mg/kg) administration was used for the estimation of cardioprotective potential for 5 weeks. Afterward, biochemical parameter, electrolyte, antioxidant, cytokines and inflammatory parameters were estimated. Kudzu vine remarkably improved the body weight and declined the heart weight. Kudzu vine extract significantly (P <0.001) suppressed the level of CK-MB, LDH, CK, cTnT and BNP. Kudzu vine significantly (P <0.001) improved the level of Na+, Ca2+, K+ and Cl− and decreased the level of urea, magnesium, total protein, albumin and globulin. Kudzu vine remarkably altered the lipid and antioxidant parameters. Additionally, Kudzu vine extract suppressed the level of TNF-α, IL-1β, IL-6 and improved the level of TGF-β, IL-10. DOX-induced cardiac dysfunction exhibited the altered relative abundance of different bacteria and Kudzu vine remarkably restore the relative abundance of different bacteria. Therefore, Kudzu vine extract has beneficial effects against DOX-induced cardiac toxicity via alteration of gut microbiota.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"27 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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