Xingchen Wang, Lin Zhang, Runkun Wang, Wenfeng Hu, Jialin Sun, Lei Wang
Panax notoginseng saponins (PNS) have excellent effects on treating cardiovascular and cerebrovascular diseases, tumors and organ interstitial diseases. This study assessed the effect of PNS on renal interstitial fibrosis (RIF). Four groups of models were firstly created, which included; renal failure model, healthy group, model group, PNS group, and then established TLR4 inhibitor group (TAK-242 group), TLR4 activator group (LPS group), combined PNS and TLR4 inhibitor group (PNS+ TAK-242 group) and combined PNS and TLR4 agonist (PNS+LPS group). TLR4/NF- κ B signaling pathway and regulatory mechanism for PNS were observed along with analysis of TGF- β 1, α -SMA, Collagen I and FN expressions in kidney tissues. The PNS significantly inhibited changes of renal interstitial fibrosis in rats with renal failure, and this process was related to decreased expressions of TLR4 and NF- κ B. The PNS also inhibited the expression of TLR4, and expressions of TGF- β 1, α -SMA, Collagen I and FN were down-regulated in kidney cells after using TAK-242, especially in the PNS+TAK-242 group. The PNS can thus significantly inhibit the process of renal fibrosis, and this process is due to the fact that, PNS inhibits the activity of TLR4/NF- κ B signaling pathway to down-regulate the expressions of fibrosis-related factors TGF- β 1, α -SMA, etc., in exerting its effects.
{"title":"Improvement on renal interstitial fibrosis in rats with renal failure via TLR4/NF-<i>κ</i>B signaling pathway by means of panax notoginseng saponins","authors":"Xingchen Wang, Lin Zhang, Runkun Wang, Wenfeng Hu, Jialin Sun, Lei Wang","doi":"10.1166/mex.2023.2546","DOIUrl":"https://doi.org/10.1166/mex.2023.2546","url":null,"abstract":"Panax notoginseng saponins (PNS) have excellent effects on treating cardiovascular and cerebrovascular diseases, tumors and organ interstitial diseases. This study assessed the effect of PNS on renal interstitial fibrosis (RIF). Four groups of models were firstly created, which included; renal failure model, healthy group, model group, PNS group, and then established TLR4 inhibitor group (TAK-242 group), TLR4 activator group (LPS group), combined PNS and TLR4 inhibitor group (PNS+ TAK-242 group) and combined PNS and TLR4 agonist (PNS+LPS group). TLR4/NF- κ B signaling pathway and regulatory mechanism for PNS were observed along with analysis of TGF- β 1, α -SMA, Collagen I and FN expressions in kidney tissues. The PNS significantly inhibited changes of renal interstitial fibrosis in rats with renal failure, and this process was related to decreased expressions of TLR4 and NF- κ B. The PNS also inhibited the expression of TLR4, and expressions of TGF- β 1, α -SMA, Collagen I and FN were down-regulated in kidney cells after using TAK-242, especially in the PNS+TAK-242 group. The PNS can thus significantly inhibit the process of renal fibrosis, and this process is due to the fact that, PNS inhibits the activity of TLR4/NF- κ B signaling pathway to down-regulate the expressions of fibrosis-related factors TGF- β 1, α -SMA, etc., in exerting its effects.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"105 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135763667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abnormal myocardial metabolism is the leading cause of diabetic cardiomyopathy (DCM). Astragalus polysaccharide (APS) combination with liposome nanoparticles (APS-nano) exhibits greater efficacy. Therefore, this study assessed regulatory effect of APS-nano on fatty acid translocase (FAT)/CD36. Fifty SD rats were assigned into control group, model group, APS group, APS-nano group, and FAT/CD36 inhibitor group ( n =10, each group), followed by analysis of FAT/CD36 mRNA, protein levels, and glucocorticoid receptor expression. APS-nano group rats had highest level of insulin among all groups and lowest blood sugar. The content of Total cholesterol (TC), triglyceride (TG) and Free Fatty Acid (FFA) in APS-nano group was lower than APS and model groups ( P = 0.000), with higher TC and FFA than control group ( P <0.001), while insulin, blood sugar, TC, TG and FFA in the APS group were lower than model group ( P = 0.000). FAT/CD36 mRNA in the model, APS, and APS-nano groups decreased to varying degrees ( P <0.01). Administration of APS-nano greatly increased glucocorticoid receptor ( P <0.01). APS-nano can regulate FAT/CD36 expression and improve fatty acid metabolism, thereby lowering myocardial tissue metabolism and inhibiting glucocorticoid receptor levels.
{"title":"Astragalus polysaccharide liposome nanoparticles mediate fatty acid translocase (FAT)/CD36 to improve fatty acid metabolism and regulate glucocorticoid receptor levels in diabetic cardiomyopathy","authors":"Yuqin Ji, Didi Zhu, Shuchao Qin, Yuanqi Yang","doi":"10.1166/mex.2023.2553","DOIUrl":"https://doi.org/10.1166/mex.2023.2553","url":null,"abstract":"Abnormal myocardial metabolism is the leading cause of diabetic cardiomyopathy (DCM). Astragalus polysaccharide (APS) combination with liposome nanoparticles (APS-nano) exhibits greater efficacy. Therefore, this study assessed regulatory effect of APS-nano on fatty acid translocase (FAT)/CD36. Fifty SD rats were assigned into control group, model group, APS group, APS-nano group, and FAT/CD36 inhibitor group ( n =10, each group), followed by analysis of FAT/CD36 mRNA, protein levels, and glucocorticoid receptor expression. APS-nano group rats had highest level of insulin among all groups and lowest blood sugar. The content of Total cholesterol (TC), triglyceride (TG) and Free Fatty Acid (FFA) in APS-nano group was lower than APS and model groups ( P = 0.000), with higher TC and FFA than control group ( P <0.001), while insulin, blood sugar, TC, TG and FFA in the APS group were lower than model group ( P = 0.000). FAT/CD36 mRNA in the model, APS, and APS-nano groups decreased to varying degrees ( P <0.01). Administration of APS-nano greatly increased glucocorticoid receptor ( P <0.01). APS-nano can regulate FAT/CD36 expression and improve fatty acid metabolism, thereby lowering myocardial tissue metabolism and inhibiting glucocorticoid receptor levels.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"104 9","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135763672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haibo He, Wenxin Wu, Jun He, Xiaotao Su, Qianhuan Gui
This study assesses the effect of bone marrow derived mesenchymal stem cells (BMSC) on chemotherapy resistance of osteosarcoma. 60 SPF mice were randomly separated into control group and model group. Serum alkaline phosphatase (ALP), Calcium (Ca), Phosphorus (P) and tumor formation rate were observed. The osteosarcoma tissues were taken to construct drug-resistant osteosarcoma cell lines. Control group, 5-fluorouracil (5-Fu) group, BMSC group, 5-Fu+BMSC group, and then set 5-Fu+BMSC+agonist group, 5-Fu+BMSC+inhibitor group, respectively. Osteosarcoma cell sensitivity to 5-Fu (IC50) and β -catenin/p- β -catenin expression were examined. Compared with control group, the ALP level and tumor formation rate in model group were higher and P level was remarkably lower. Ca level showed no difference between two groups ( P >0.05). The 5-Fu+BMSC group and 5-Fu group had the highest IC50 levels and the control group and BMSC group had the lowest IC50 levels. The β -catenin/p- β -catenin expressions were the highest in 5-Fu+BMSC group and 5-Fu group, and their expressions in control group and BMSC group were the lowest. 5-Fu+BMSC+agonist group showed higher β -catenin, p- β -catenin and IC50 levels, which are lower in 5-Fu+BMSC+inhibitor group. Endogenous BMSC can promote the chemotherapy resistance of osteosarcoma. They can promote β -catenin and β -catenin phosphorylation level, down-regulate osteosarcoma cell sensitivity to 5-Fu, and then promote drug resistance. Therefore, β -catenin signaling can be used as a target to reverse the resistance of osteosarcoma cells to 5-Fu.
{"title":"The effect of endogenous bone marrow derived mesenchymal stem cells on the activity of <i>β</i>-catenin signaling pathway on chemotherapy resistance of osteosarcoma","authors":"Haibo He, Wenxin Wu, Jun He, Xiaotao Su, Qianhuan Gui","doi":"10.1166/mex.2023.2552","DOIUrl":"https://doi.org/10.1166/mex.2023.2552","url":null,"abstract":"This study assesses the effect of bone marrow derived mesenchymal stem cells (BMSC) on chemotherapy resistance of osteosarcoma. 60 SPF mice were randomly separated into control group and model group. Serum alkaline phosphatase (ALP), Calcium (Ca), Phosphorus (P) and tumor formation rate were observed. The osteosarcoma tissues were taken to construct drug-resistant osteosarcoma cell lines. Control group, 5-fluorouracil (5-Fu) group, BMSC group, 5-Fu+BMSC group, and then set 5-Fu+BMSC+agonist group, 5-Fu+BMSC+inhibitor group, respectively. Osteosarcoma cell sensitivity to 5-Fu (IC50) and β -catenin/p- β -catenin expression were examined. Compared with control group, the ALP level and tumor formation rate in model group were higher and P level was remarkably lower. Ca level showed no difference between two groups ( P >0.05). The 5-Fu+BMSC group and 5-Fu group had the highest IC50 levels and the control group and BMSC group had the lowest IC50 levels. The β -catenin/p- β -catenin expressions were the highest in 5-Fu+BMSC group and 5-Fu group, and their expressions in control group and BMSC group were the lowest. 5-Fu+BMSC+agonist group showed higher β -catenin, p- β -catenin and IC50 levels, which are lower in 5-Fu+BMSC+inhibitor group. Endogenous BMSC can promote the chemotherapy resistance of osteosarcoma. They can promote β -catenin and β -catenin phosphorylation level, down-regulate osteosarcoma cell sensitivity to 5-Fu, and then promote drug resistance. Therefore, β -catenin signaling can be used as a target to reverse the resistance of osteosarcoma cells to 5-Fu.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"107 9","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135763786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sihan Chen, Huiqi Hu, Wenting Jiang, Yuyun Zhang, Xiaohu Luo, Jiayi Peng, Le Li, Deshuai Zhen, Hanqing Wang
A novel near-infrared induced upconversion sensor for the detection of uranyl ions was successfully constructed using β -NaYF4: Yb, Tm, Gd upconversion nanoparticles (UCNPs) and specific DNAzyme. Characterization of the UCNPs@DNAzyme was conducted using XRD, TEM, FT-IR and fluorescence testing, and it was shown that the β -NaYF4: Yb, Tm, Gd nanoparticles were successfully prepared (∼27 nm). Upon 980 nm excitation, the UCNPs@DNAzyme emitted green light at λ =475 nm. Furthermore, Förster resonance energy transfer was observed between the UCNPs@DNAzyme (doner) and uranyl (acceptor) present on the substrate chain. This resulted in fluorescence quenching, which was used to quantify the concentration of uranyl. The developed method was found to be highly selective and sensitive, with a detection limit as low as 43 nM. This method also demonstrated excellent specificity and sensitivity for the determination of uranyl in water samples, indicating its potential application in near-infrared fluorescence detection and imaging of uranyl in vivo .
{"title":"DNAzyme modified NaYF<sub>4</sub>: Yb, Tm, Gd for near-infrared fluorescence detection of uranyl","authors":"Sihan Chen, Huiqi Hu, Wenting Jiang, Yuyun Zhang, Xiaohu Luo, Jiayi Peng, Le Li, Deshuai Zhen, Hanqing Wang","doi":"10.1166/mex.2023.2580","DOIUrl":"https://doi.org/10.1166/mex.2023.2580","url":null,"abstract":"A novel near-infrared induced upconversion sensor for the detection of uranyl ions was successfully constructed using β -NaYF4: Yb, Tm, Gd upconversion nanoparticles (UCNPs) and specific DNAzyme. Characterization of the UCNPs@DNAzyme was conducted using XRD, TEM, FT-IR and fluorescence testing, and it was shown that the β -NaYF4: Yb, Tm, Gd nanoparticles were successfully prepared (∼27 nm). Upon 980 nm excitation, the UCNPs@DNAzyme emitted green light at λ =475 nm. Furthermore, Förster resonance energy transfer was observed between the UCNPs@DNAzyme (doner) and uranyl (acceptor) present on the substrate chain. This resulted in fluorescence quenching, which was used to quantify the concentration of uranyl. The developed method was found to be highly selective and sensitive, with a detection limit as low as 43 nM. This method also demonstrated excellent specificity and sensitivity for the determination of uranyl in water samples, indicating its potential application in near-infrared fluorescence detection and imaging of uranyl in vivo .","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135763389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huan-Jin Ma, Li Lin, Ze-Bin Chen, Sheng-Guang Xu, Yu Li, Rui Zhang, Sheng-Yue Yi
Biochar is a carbon-rich soil conditioner produced from pyrolysis of biomass, it has been widely used to enhance soil quality because of its physical adsorption as well as water and fertilizer conservation functions. This work aimed to improve the soil quality of continuously cropped flue-cured tobacco fields using biochar prepared from agricultural waste. To explore the impact of reduced compound fertilizer with biochar application on nutrients, phenolic acid contents and fungi diversity in the rhizosphere soil, 4 treatments were set: regular compound fertilizer application (T1), and biochar with reduced compound fertilizer in different proportions (T2, T3, T4, with 100, 75, 50% of compound fertilizer, respectively). The physicochemical properties of the prepared biochar were characterized and observed using electron microscopy. The results indicated that, a noticeable increase in the content of soil organic matter (SOM) and soil organic carbon (SOC) in T2 treatment compared to T1 treatment. Moreover, T2 treatment demonstrated a significant improvement in the contents of alkali-hydrolyzable nitrogen (AN), available phosphorus (AP), and available potassium (AK), with increases of 9.29%, 15.85% and 25.42% compared to T1 treatment, respectively. While a gradual decrease in soil AN, AP, and AK content was observed with the reduction of compound fertilizer application (T2–T4), there was no obvious difference between T3 and T1. For total phenolic acid, the content in T2 treatment significantly decreased by 35.99% compared to T1 treatment, while T3 and T4 treatments showed significant reductions of 15.30 and 18.73% respectively, compared to T2 treatment. Biochar application could enhance the fungal community’s abundance and diversity in the rhizosphere soil. Fungal community exhibited the highest richness under T3 treatment, while the relative abundance of Fusarium and Mortierella reduced as the decrease of compound fertilizer (T2–T4). In conclusion, the reduced compound fertilizer with biochar application could reduce nutrient loss, phenolic acids accumulation, and improving the abundance of fungal community in the rhizosphere soil. This paper provides a reference for biochar combined with compound fertilizer to improve soil from the regulation of allelochemicals and soil fungi.
{"title":"Biochar preparation and its effects with reduced compound fertilizer on nutrients, phenolic acid and fungal community in tobacco rhizosphere soil","authors":"Huan-Jin Ma, Li Lin, Ze-Bin Chen, Sheng-Guang Xu, Yu Li, Rui Zhang, Sheng-Yue Yi","doi":"10.1166/mex.2023.2574","DOIUrl":"https://doi.org/10.1166/mex.2023.2574","url":null,"abstract":"Biochar is a carbon-rich soil conditioner produced from pyrolysis of biomass, it has been widely used to enhance soil quality because of its physical adsorption as well as water and fertilizer conservation functions. This work aimed to improve the soil quality of continuously cropped flue-cured tobacco fields using biochar prepared from agricultural waste. To explore the impact of reduced compound fertilizer with biochar application on nutrients, phenolic acid contents and fungi diversity in the rhizosphere soil, 4 treatments were set: regular compound fertilizer application (T1), and biochar with reduced compound fertilizer in different proportions (T2, T3, T4, with 100, 75, 50% of compound fertilizer, respectively). The physicochemical properties of the prepared biochar were characterized and observed using electron microscopy. The results indicated that, a noticeable increase in the content of soil organic matter (SOM) and soil organic carbon (SOC) in T2 treatment compared to T1 treatment. Moreover, T2 treatment demonstrated a significant improvement in the contents of alkali-hydrolyzable nitrogen (AN), available phosphorus (AP), and available potassium (AK), with increases of 9.29%, 15.85% and 25.42% compared to T1 treatment, respectively. While a gradual decrease in soil AN, AP, and AK content was observed with the reduction of compound fertilizer application (T2–T4), there was no obvious difference between T3 and T1. For total phenolic acid, the content in T2 treatment significantly decreased by 35.99% compared to T1 treatment, while T3 and T4 treatments showed significant reductions of 15.30 and 18.73% respectively, compared to T2 treatment. Biochar application could enhance the fungal community’s abundance and diversity in the rhizosphere soil. Fungal community exhibited the highest richness under T3 treatment, while the relative abundance of Fusarium and Mortierella reduced as the decrease of compound fertilizer (T2–T4). In conclusion, the reduced compound fertilizer with biochar application could reduce nutrient loss, phenolic acids accumulation, and improving the abundance of fungal community in the rhizosphere soil. This paper provides a reference for biochar combined with compound fertilizer to improve soil from the regulation of allelochemicals and soil fungi.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"26 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135763390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asiaticoside is one of the most classic traditional Chinese medicines. The interaction between Receptor activator of nuclear factor κ B (RANK) and Receptor Activator of Nuclear Factor- κ B Ligand (RANKL) activates a series of intracellular signaling pathways. Our research explored the mechanism of asiaticoside inhibiting osteoclast differentiation and repair of steroid-induced osteonecrosis of femoral head (SIONFH) through RANKL pathway. Asiaticoside’s effect on osteoclasts was determined by Microneedle Therapy System (MTS) method, and the number of Tartrate resistant acid phosphatase (TRAcP) multinucleated cells (3 nuclei) was quantitatively analyzed. Under different dose treatments, the changes of bone function were detected by eroded area and TRAcP staining on hydroxyapatite-coated plate, and the expression of different osteocyte formation and osteogenic differentiation were detected. Asiaticoside weakened the osteoclast function induced by RANKL. When the concentration of asiaticoside was 10 μ mol/L, osteoclast formation regulatory genes (NFATc1 and Acp5) and osteoclast cell function-related genes (AP-1 and P65) were significantly downregulated in the presence of RANKL. Asiaticoside (10 μ mol/L) significantly decreased calcium oscillations induced by RANKL via preventing RANKL-mediated NF- κ B activation and Ca 2+ oscillation.
积雪草苷是最经典的中药之一。核因子κ B受体激活因子(Receptor activator of nuclear factor - κ B Ligand, RANK)与核因子κ B受体激活因子(Receptor activator of nuclear factor - κ B)之间的相互作用激活了一系列细胞内信号通路。我们的研究探讨了积雪草苷通过RANKL途径抑制破骨细胞分化和激素性股骨头坏死(SIONFH)修复的机制。采用微针治疗系统(MTS)法检测积雪草苷对破骨细胞的影响，定量分析酒石酸盐耐酸性磷酸酶(TRAcP)多核细胞(3核)数量。通过羟基磷灰石包被板侵蚀面积和TRAcP染色检测不同剂量下骨功能的变化，检测不同骨细胞形成和成骨分化的表达。积雪草总苷对RANKL诱导的破骨细胞功能有减弱作用。当积雪草苷浓度为10 μ mol/L时，破骨细胞形成调控基因(NFATc1和Acp5)和破骨细胞功能相关基因(AP-1和P65)在RANKL存在下显著下调。积雪草苷(10 μ mol/L)通过抑制RANKL介导的NF- κ B活化和ca2 +振荡，显著降低RANKL诱导的钙振荡。
{"title":"Natural drug asiaticoside inhibits osteoclast differentiation and promotes repair of steroid-induced osteonecrosis of femoral head via RANKL pathway","authors":"Haijian Wu, Bowen Guo, Hongfa Zhong, Hui Ying","doi":"10.1166/mex.2023.2536","DOIUrl":"https://doi.org/10.1166/mex.2023.2536","url":null,"abstract":"Asiaticoside is one of the most classic traditional Chinese medicines. The interaction between Receptor activator of nuclear factor κ B (RANK) and Receptor Activator of Nuclear Factor- κ B Ligand (RANKL) activates a series of intracellular signaling pathways. Our research explored the mechanism of asiaticoside inhibiting osteoclast differentiation and repair of steroid-induced osteonecrosis of femoral head (SIONFH) through RANKL pathway. Asiaticoside’s effect on osteoclasts was determined by Microneedle Therapy System (MTS) method, and the number of Tartrate resistant acid phosphatase (TRAcP) multinucleated cells (3 nuclei) was quantitatively analyzed. Under different dose treatments, the changes of bone function were detected by eroded area and TRAcP staining on hydroxyapatite-coated plate, and the expression of different osteocyte formation and osteogenic differentiation were detected. Asiaticoside weakened the osteoclast function induced by RANKL. When the concentration of asiaticoside was 10 μ mol/L, osteoclast formation regulatory genes (NFATc1 and Acp5) and osteoclast cell function-related genes (AP-1 and P65) were significantly downregulated in the presence of RANKL. Asiaticoside (10 μ mol/L) significantly decreased calcium oscillations induced by RANKL via preventing RANKL-mediated NF- κ B activation and Ca 2+ oscillation.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"105 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135763806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bo Xiao, Samaneh Kiani, Reza Toushmalani, Akram Zangeneh, Mohammad Mahdi Zangeneh, Samaneh Goorani, Hassan Morovvati
Repairing cutaneous wounds imposes a lot of cost on the country every year. Also, cutaneous wounds healing is delayed for various reasons such as the accumulation of free radicals, hospital infections, lack of sufficient moisture, traditional dressings, etc. Therefore, treatment methods need to be reviewed. In recent years, a wide range of copper ointments or silver-releasing compounds have been prepared and used. These ointments have overcome some of the problems associated with copper preparation. In the current work, an environment-friendly a green procedure was described for fabrication of CuO NPs by using sodium lignosulfonate as a capping/stabilizing reagent under alkaline medium. The characterization of prepared CuO NPs@NaLS were analysed using SEM, EdaX, ICP-OES, elemental mapping and TEM data. The FE-SEM and TEM results show that the CuO NPs@NaLS formed as spherical NPs with size of 20–30 nm. According to the elementaling map analysis, the Cu and C were existence with good dispersion that detected by EDX in the composite. In this study, ointments with a concentration of 0.1% were prepared from nanoparticles based on basal ointment (as the main substance that keeps the medicine on the wound) and used as a topical application. In this way, on the second day, angiogenesis (angiogenesis) and the severity of edema and edema, on the fifth day, fibroblasts, and on the tenth, fibrocytes and collagen fibers were examined. According to the present study results, the skin wound area in the rats treated with topical use of NPs was significantly reduced, and in terms of histopathology, it showed more improvement compared to other groups. Overall, the results of this study show that recent nanoparticles can be used as a fast and effective treatment for skin wounds healing.
{"title":"Biosynthesis of copper oxide nanoparticles (CuO NPs) using sodium lignosulfonate and evaluation of its cutaneous wound healing properties","authors":"Bo Xiao, Samaneh Kiani, Reza Toushmalani, Akram Zangeneh, Mohammad Mahdi Zangeneh, Samaneh Goorani, Hassan Morovvati","doi":"10.1166/mex.2023.2543","DOIUrl":"https://doi.org/10.1166/mex.2023.2543","url":null,"abstract":"Repairing cutaneous wounds imposes a lot of cost on the country every year. Also, cutaneous wounds healing is delayed for various reasons such as the accumulation of free radicals, hospital infections, lack of sufficient moisture, traditional dressings, etc. Therefore, treatment methods need to be reviewed. In recent years, a wide range of copper ointments or silver-releasing compounds have been prepared and used. These ointments have overcome some of the problems associated with copper preparation. In the current work, an environment-friendly a green procedure was described for fabrication of CuO NPs by using sodium lignosulfonate as a capping/stabilizing reagent under alkaline medium. The characterization of prepared CuO NPs@NaLS were analysed using SEM, EdaX, ICP-OES, elemental mapping and TEM data. The FE-SEM and TEM results show that the CuO NPs@NaLS formed as spherical NPs with size of 20–30 nm. According to the elementaling map analysis, the Cu and C were existence with good dispersion that detected by EDX in the composite. In this study, ointments with a concentration of 0.1% were prepared from nanoparticles based on basal ointment (as the main substance that keeps the medicine on the wound) and used as a topical application. In this way, on the second day, angiogenesis (angiogenesis) and the severity of edema and edema, on the fifth day, fibroblasts, and on the tenth, fibrocytes and collagen fibers were examined. According to the present study results, the skin wound area in the rats treated with topical use of NPs was significantly reduced, and in terms of histopathology, it showed more improvement compared to other groups. Overall, the results of this study show that recent nanoparticles can be used as a fast and effective treatment for skin wounds healing.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"108 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135763936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jingchun Guo, Ran Wang, Jiao Yu, Zhilin Zhang, Xucheng Fu
Recently, ultrathin two-dimensional (2D) noble-metal-based nanomaterials have gained a lot of attention due to their interesting physicochemical properties and widespread promising applications. In particular, 2D Pt-based intermetallic nanoplates (IMNPs) have been widely studied in cathodic oxygen reduction reaction (ORR), anodic methanol, formic acid, and ethanol oxidation reactions (MOR, FAOR, and EOR) of fuel cells (FCs). In this article, we first give a fundamental understanding of the FCs. Subsequently, several modulation strategies for 2D Pt-based IMNPs including alloying, doping, facet engineering, heterogeneous structure construction, defect and interface engineering, as well as their applications in ORR, MOR, FAOR, and EOR are briefly summarized. Finally, we discuss the current challenges and give our perspectives on the synthesis of high-performance 2D Pt-based IMNPs toward FCs.
{"title":"Modulation of two-dimensional Pt-based intermetallic nanoplates for fuel cells","authors":"Jingchun Guo, Ran Wang, Jiao Yu, Zhilin Zhang, Xucheng Fu","doi":"10.1166/mex.2023.2541","DOIUrl":"https://doi.org/10.1166/mex.2023.2541","url":null,"abstract":"Recently, ultrathin two-dimensional (2D) noble-metal-based nanomaterials have gained a lot of attention due to their interesting physicochemical properties and widespread promising applications. In particular, 2D Pt-based intermetallic nanoplates (IMNPs) have been widely studied in cathodic oxygen reduction reaction (ORR), anodic methanol, formic acid, and ethanol oxidation reactions (MOR, FAOR, and EOR) of fuel cells (FCs). In this article, we first give a fundamental understanding of the FCs. Subsequently, several modulation strategies for 2D Pt-based IMNPs including alloying, doping, facet engineering, heterogeneous structure construction, defect and interface engineering, as well as their applications in ORR, MOR, FAOR, and EOR are briefly summarized. Finally, we discuss the current challenges and give our perspectives on the synthesis of high-performance 2D Pt-based IMNPs toward FCs.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"107 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135763939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guosheng Xing, Ruihua Kong, Shuli Yang, Ruixue Ma, Zhiguo Sun
To evaluate the efficacy of nanoparticle-loaded cisplatin (DDP) for treating laryngeal cancer (LC), we developed a delivery system for targeted DDP delivery using gold nanoparticles (GNPs). FaDu cells were cultured to assess the permeability and antitumor efficacy of DDP@GNP, and an LC rat model was constructed to further investigate its antitumor efficiency. In vitro experiments revealed that the DDP@GPN had a particle size ranging from 80–90 nm and significantly prolonged the half-life of DDP by nearly 50-fold. Additionally, DDP@GNP demonstrated excellent targeting and penetration ability in FaDu cells, thereby effectively enhancing apoptosis and inhibiting the invasive properties of tumor cells. Furthermore, DDP@GNP enhanced the radiosensitivity of FaDu cells, thus enhancing the efficacy of radiotherapy. In vivo experiments also showed that the combination of DDP@GNP with radiotherapy significantly reduced tumor volume. Therefore, DDP@GNP shows immense potential for enhancing the efficacy of chemoradiotherapy in LC.
{"title":"Application of nanogold-cisplatin drug delivery system in laryngeal cancer chemoradiotherapy","authors":"Guosheng Xing, Ruihua Kong, Shuli Yang, Ruixue Ma, Zhiguo Sun","doi":"10.1166/mex.2023.2539","DOIUrl":"https://doi.org/10.1166/mex.2023.2539","url":null,"abstract":"To evaluate the efficacy of nanoparticle-loaded cisplatin (DDP) for treating laryngeal cancer (LC), we developed a delivery system for targeted DDP delivery using gold nanoparticles (GNPs). FaDu cells were cultured to assess the permeability and antitumor efficacy of DDP@GNP, and an LC rat model was constructed to further investigate its antitumor efficiency. In vitro experiments revealed that the DDP@GPN had a particle size ranging from 80–90 nm and significantly prolonged the half-life of DDP by nearly 50-fold. Additionally, DDP@GNP demonstrated excellent targeting and penetration ability in FaDu cells, thereby effectively enhancing apoptosis and inhibiting the invasive properties of tumor cells. Furthermore, DDP@GNP enhanced the radiosensitivity of FaDu cells, thus enhancing the efficacy of radiotherapy. In vivo experiments also showed that the combination of DDP@GNP with radiotherapy significantly reduced tumor volume. Therefore, DDP@GNP shows immense potential for enhancing the efficacy of chemoradiotherapy in LC.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"26 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135763391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, nipple discharge patients were selected as subjects to evaluate the diagnostic value of ductoscopy and the tumor marker CEA expression in early occulted breast cancer. A retrospective analysis was performed on 121 pathological nipple discharge patients who received treatment in our hospital. Thirty-two parturients who delivered normally in our hospital during the same period were randomly selected to be included in the control group. Samples of the subjects’ milk and nipple discharge were collected. The contents of tumor markers CEA, CA153, and CA125 were measured in the nipple discharge and milk using nanomagnetic beads combined with chemiluminescence immunoassay (CLIA). The findings from ductoscopy were documented and compared to the pathological results obtained after surgery. Nanomagnetic beads combined with CLIA can enhance the efficiency and stability of detection. CEA and CA125 levels increased in the group with breast lesions, and reached their highest levels in the group with malignant breast diseases. The positive prediction rates of CA153 and CEA in breast cancer were significantly higher than in the benign lesion group ( P < 0005). Univariate analysis showed that bloody nipple discharge or III ∼ IV breast duct lesions identified as high-risk factors associated with breast cancer. The sensitivity of endoscopy for diagnosing malignant lesions was 90.70%, while the sensitivity of CEA+ endoscopy was 95.35%. Ductoscopy has high sensitivity in the diagnosis of early occult breast cancer. The sensitivity and specificity of diagnosis could be significantly improved by combining tumor marker CEA expression with ductoscopy.
{"title":"The role of nanomagnetic beads in diagnosis of early breast cancer by ductoscopy combined with CEA expression of tumor marker","authors":"Jin Li, Huyan Su, Xiaofeng Li","doi":"10.1166/mex.2023.2535","DOIUrl":"https://doi.org/10.1166/mex.2023.2535","url":null,"abstract":"In this study, nipple discharge patients were selected as subjects to evaluate the diagnostic value of ductoscopy and the tumor marker CEA expression in early occulted breast cancer. A retrospective analysis was performed on 121 pathological nipple discharge patients who received treatment in our hospital. Thirty-two parturients who delivered normally in our hospital during the same period were randomly selected to be included in the control group. Samples of the subjects’ milk and nipple discharge were collected. The contents of tumor markers CEA, CA153, and CA125 were measured in the nipple discharge and milk using nanomagnetic beads combined with chemiluminescence immunoassay (CLIA). The findings from ductoscopy were documented and compared to the pathological results obtained after surgery. Nanomagnetic beads combined with CLIA can enhance the efficiency and stability of detection. CEA and CA125 levels increased in the group with breast lesions, and reached their highest levels in the group with malignant breast diseases. The positive prediction rates of CA153 and CEA in breast cancer were significantly higher than in the benign lesion group ( P < 0005). Univariate analysis showed that bloody nipple discharge or III ∼ IV breast duct lesions identified as high-risk factors associated with breast cancer. The sensitivity of endoscopy for diagnosing malignant lesions was 90.70%, while the sensitivity of CEA+ endoscopy was 95.35%. Ductoscopy has high sensitivity in the diagnosis of early occult breast cancer. The sensitivity and specificity of diagnosis could be significantly improved by combining tumor marker CEA expression with ductoscopy.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"104 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135763674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：